Publications

molecular analysis of anthocyanin-related genes in ornamental cabbage.	ornamental cabbage (brassica oleracea var. acephala) is a winter-grown and important decorative plant of the brassicaceae family, which displays an exceptional coloration in the central leaves of the rosette. anthocyanins are the key determinant of the red, purple and blue colors of vegetative and reproductive parts of many plant species including ornamental cabbage. total anthocyanin content was measured spectrophotometrically and the highest anthocyanin content detected in the red followed by ...	2017	29232522
transcriptome analysis and metabolic profiling of green and red kale (brassica oleracea var. acephala) seedlings.	kale (brassica oleracea var. acephala) is a rich source of numerous health-benefiting compounds, including vitamins, glucosinolates, phenolic compounds, and carotenoids. however, the genetic resources for exploiting the phyto-nutritional traits of kales are limited. to acquire precise information on secondary metabolites in kales, we performed a comprehensive analysis of the transcriptome and metabolome of green and red kale seedlings. kale transcriptome datasets revealed 37,149 annotated genes ...	2018	28958560
flavonoids and ros play opposing roles in mediating pollination in ornamental kale (brassica oleracea var. acephala).		2017	28827168
kale borack1 is involved in the plant response to salt stress and peronospora brassicae gaumann.	the receptor for activated c kinase 1 (rack1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (wd) repeat structure. compelling evidence indicates that rack1 can interact with many signal molecules and affect different signal transduction pathways. in this study, a kale (brassica oleracea var. acephala f.tricolor) rack1 gene (borack1) was cloned by rt-pcr. the amino acid sequence of borack1 had seven wd repeats in which there were typical gh (glycine-histidine) and wd ...	2017	28411489
potential of legume-brassica intercrops for forage production and green manure: encouragements from a temperate southeast european environment.	legumes and brassicas have much in common: importance in agricultural history, rich biodiversity, numerous forms of use, high adaptability to diverse farming designs, and various non-food applications. rare available resources demonstrate intercropping legumes and brassicas as beneficial to both, especially for the latter, profiting from better nitrogen nutrition. our team aimed at designing a scheme of the intercrops of autumn- and spring-sown annual legumes with brassicas for ruminant feeding ...	2017	28326095
effects of experimental design and nitrogen on cabbage butterfly oviposition.	to test the prediction that p. rapae egg densities increase with n fertilizer in large-scale systems as they do in model systems with potted plants, we used field experiments with brassica oleracea var. acephala l. (collards and kale) planted in pots or large field plots, and treated with different levels of nitrogen fertilizer. in small-scale field experiments with potted kale and collards, egg densities were significantly higher on plants with high n than those with low n. but in larger scale ...	1989	28313109
parasitism rates and sex ratios of a parasitoid wasp: effects of herbivore and plant quality.	we studied interactions among collards, brassica oleracea var. acephala, the diamondback moth (dbm), plutella xylostella (lepidoptera: yponomeutidae) and its parasitoid diadegma insulare (hymenoptera: ichneumonidae) by manipulating plant nitrogen (n) concentrations in field and laboratory experiments. parasitoid abundance strongly reflected dbm abundance and was related to total leaf n. parasitism rates were high (70.7%) and density-independent. wasp sex ratios varied markedly (3-93% female) in ...	1990	28313016
intake of kale suppresses postprandial increases in plasma glucose: a randomized, double-blind, placebo-controlled, crossover study.	kale (brassica oleracea var. acephala), a vegetable in the family brassicaceae, has beneficial effects on health, including hypoglycemic effects. in our previous study with a limited number of subjects, intake of kale-containing food at a dose of 14 g decreased postprandial plasma glucose levels. in the present study, the effective dose of kale-containing food was investigated in a randomized, double-blind, placebo-controlled, crossover trial. the trial was conducted on 42 japanese subjects aged ...	2016	27882216
modification of leaf glucosinolate contents in brassica oleracea by divergent selection and effect on expression of genes controlling glucosinolate pathway.	modification of the content of secondary metabolites opens the possibility of obtaining vegetables enriched in these compounds related to plant defense and human health. we report the first results of a divergent selection for glucosinolate (gsl) content of the three major gsl in leaves: sinigrin (sin), glucoiberin (gib), and glucobrassicin (gbs) in order to develop six kale genotypes (brassica oleracea var. acephala) with high (hsin, higib, hgbs) and low (lsin, lgib, lgbs) content. the aims wer ...	2016	27471510
the influence of atmospheric particles on the elemental content of vegetables in urban gardens of sao paulo, brazil.	although urban horticulture provides multiple benefits to society, the extent to which these vegetables are contaminated by the absorption of chemical elements derived from atmospheric deposition is unclear. this study was designed to evaluate the influence of air pollution on leafy vegetables in community gardens of sao paulo, brazil. vegetable seedlings of brassica oleracea var. acephala (collard greens) and spinacia oleracea (spinach) obtained in a non-polluted rural area and growing in vesse ...	2016	27249450
amiprophosmethyl-induced efficient in vitro production of polyploids in raphanobrassica with the aid of aminoethoxyvinylglycine (avg) in the culture medium.	optimum conditions for obtaining tetraploid were investigated in raphanobrassica, the intergeneric hybrid between radish (raphanus sativus) and kale (brassica oleracea var. acephala) by treating in vitro plants with an anti-mitotic agent, amiprophosmethyl (apm). initially, no tetraploids but hexaploids and octaploids were induced by the treatments. although the leaves of these polyploids of raphanobrassica showed chlorosis during subcultures in in vitro conditions, the chlorosis could be success ...	2015	26719742
arsenic and lead uptake by brassicas grown on an old orchard site.	arugula (eruca sativa) and collards (brassica oleracea var. acephala), were grown at a former orchard where soils had been variably contaminated by lead arsenate pesticides. to test for the effect of compost on as and pb transfer into plants, compost was added (0, 5, and 10% dw) to five plots representing a wide range of soil pb and as. arugula accumulated about 5 times higher as concentrations in above-ground tissues than collards, with high variability in individual plant concentrations. soil ...	2015	26280370
isolation and characterization of a j domain protein that interacts with arc1 from ornamental kale (brassica oleracea var. acephala).	a novel j domain protein, jdp1, was isolated from ornamental kale. the c-terminus of jdp1 specifically interacted with arc1, which has a conserved role in self-incompatibility signaling. armadillo (arm)-repeat containing 1 (arc1) plays a conserved role in self-incompatibility signaling across the brassicaceae and functions downstream of the s-locus receptor kinase. here, we identified a j domain protein 1 (jdp1) that interacts with arc1 using a yeast two-hybrid screen against a stigma cdna libra ...	2015	25666275
heavy metal contamination of vegetables irrigated by urban stormwater: a matter of time?	urban stormwater is a crucial resource at a time when climate change and population growth threaten freshwater supplies; but there are health risks from contaminants, such as toxic metals. it is vitally important to understand how to use this resource safely and responsibly. our study investigated the extent of metal contamination in vegetable crops irrigated with stormwater under short- and long-term conditions. we created artificially aged gardens by adding metal-contaminated sediment to soil, ...	2014	25426946
overproduction of stromal ferredoxin:nadph oxidoreductase in h2o 2-accumulating brassica napus leaf protoplasts.	the isolation of brassica napus leaf protoplasts induces reactive oxygen species generation and accumulation in the chloroplasts. an activated isoform of nadph oxidase-like protein was detected in the protoplasts and the protoplast chloroplasts. the purpose of this study is to define the nadh oxidase-like activities in the h2o2-accumulating protoplast chloroplasts. proteomic analysis of this protein revealed an isoform of ferredoxin:nadph oxidoreductase (fnr1). while leaves highly expressed the ...	2014	25255860
heterologous expression of atmyb12 in kale (brassica oleracea var. acephala) leads to high flavonol accumulation.	overexpression of arabidopsis atmyb12 transcription factor greatly increases the total phenolic and flavonol content in transgenic kale leaves. flavonoids are a diverse group of plant secondary metabolites exhibiting a number of health-promoting effects. there has been a growing interest to develop biotechnological methods for the enhanced production of flavonoids in crop plants. atmyb12 is an arabidopsis transcription factor which specifically activates flavonol synthesis and its overexpression ...	2014	24792422
rice-straw mulch reduces the green peach aphid, myzus persicae (hemiptera: aphididae) populations on kale, brassica oleracea var. acephala (brassicaceae) plants.	organic mulches, like peel and rice-straw, besides other materials affect the uv and temperature, which cause a reduction in the aphid arrival. the aim was to evaluate the effect of covering the soil with straw on the populations of the green peach aphid, myzus persicae on the kale, brassica oleracea var. acephala plants. the first experiment evaluated the direct effect of the rice-straw mulch and the second its indirect effect on aphid immigration, testing the plant characteristics that could l ...	2014	24714367
the association of gibberellin-like activity with the chloroplast fraction of leaf homogenates.	significant gibberellin-like activity has been detected in the 1000xg fraction of leaves of brassica oleracea var. acephala (canson kale) and hordeum vulgare (cv. "himalaya"). in kale and barley the qualitative pattern of activity found in the chloroplast fraction differs from that normally seen in total-leaf extracts. when expressed on a total chlorophyll or sample fresh-weight basis, approximately 16% of the gibberellin-like activity found in the leaf can be accounted for in the 1000xg or chlo ...	1968	24519602
bottom-up and top-down herbivore regulation mediated by glucosinolates in brassica oleracea var. acephala.	quantitative differences in plant defence metabolites, such as glucosinolates, may directly affect herbivore preference and performance, and indirectly affect natural enemy pressure. by assessing insect abundance and leaf damage rate, we studied the responses of insect herbivores to six genotypes of brassica oleracea var. acephala, selected from the same cultivar for having high or low foliar content of sinigrin, glucoiberin and glucobrassicin. we also investigated whether the natural parasitism ...	2014	24352843
influence of previous experience on the preference, food utilization and performance of ascia monuste orseis wild larvae (godart) (lepidoptera: pieridae) for three different hosts.	the exhaustion of food resources which occurs during the ontogenetic growth of ascia monuste orseis (godart) results in the dispersion of older larvae to nearby plants in order to complete their development, which might expose these animals to the nutritional variation of the hosts found. this study aimed to verify whether the food ingested in the beginning of the development influences the larvae host preference and whether the shift to a new host can affect the digestion and performance of a. ...	2013	23939268
novel bioresources for studies of brassica oleracea: identification of a kale myb transcription factor responsible for glucosinolate production.	plants belonging to the brassicaceae family exhibit species-specific profiles of glucosinolates (gsls), a class of defence compounds against pathogens and insects. gsls also exhibit various human health-promoting properties. among them, glucoraphanin (aliphatic 4-methylsulphinylbutyl gsl) has attracted the most attention because it hydrolyses to form a potent anticancer compound. increased interest in developing commercial varieties of brassicaceae crops with desirable gsl profiles has led to at ...	2013	23910994
a putative functional myb transcription factor induced by low temperature regulates anthocyanin biosynthesis in purple kale (brassica oleracea var. acephala f. tricolor).	the purple kale (brassica oleracea var. acephala f. tricolor) is a mutation in kales, giving the mutant phenotype of brilliant purple color in the interior. total anthocyanin analysis showed that the amount of anthocyanins in the purple kale was up to 1.73 mg g(-1) while no anthocyanin was detected in the white kale. to elucidate the molecular mechanism of the anthocyanin biosynthesis in the purple kale, we analyzed the expression of structural genes and some transcription factors associated wit ...	2012	21987119
gastroprotective activity of hydroalcoholic extract obtained from the leaves of brassica oleracea var. acephala dc in different animal models.	brassica oleracea var. acephala dc has been extensively used in brazilian traditional medicine to treat gastric ulcer.	2011	21986229
[selection of winter plant species for wetlands constructed as sewage treatment systems and evaluation of their wastewater purification potentials].	in order to establish an evaluation system for selection of winter wetland plants possessing high wastewater purification potentials in subtropics areas, designed sewage treatment experiments were carried out by introducing into the constructed wetlands 25 species of winter wetland plants. cluster analysis was performed by including harmful environment-resistant enzyme and substrate enzyme activities into the commonly applied plant screening and assessment indexes system. the obtained results in ...	2010	21090294
[potential of metarhizium anisopliae and beauveria bassiana isolates and neem oil to control the aphid lipaphis erysimi (kalt.) (hemiptera: aphididae)].	this work aimed to determine the efficiency of the entomopathogenic fungi metarhizium anisopliae and beauveria bassiana to control the aphid lipaphis erysimi (kalt.) (hemiptera: aphididae) in kale brassica oleracea var acephala d.c., as well as their compatibility with a neem oil formulation (neemseto). ten isolates of both fungi were tested and the most pathogenic ones were b. bassiana cg001 and m. anisopliae cg30 with 90% and 4.4 days, and 64% and 3.8 days of mortality and median lethal time, ...	2009	19768273
metabolic and bioactivity insights into brassica oleracea var. acephala.	seeds of brassica oleracea var. acephala (kale) were analyzed by hplc/uv-pad/msn-esi. several phenolic acids and flavonol derivatives were identified. the seeds of this b. oleracea variety exhibited more flavonol derivatives than those of tronchuda cabbage (brassica oleracea var. costata), also characterized in this paper. quercetin and isorhamnetin derivatives were found only in kale seeds. oxalic, aconitic, citric, pyruvic, malic, quinic, shikimic, and fumaric acids were the organic acids pres ...	2009	19722523
responses of some common cruciferae to root-knot nematodes.	ten cultivated plants of the family cruciferae were evaluated for susceptibility to meloidogyne arenaria race 1, m. incognita races 1 and 3, and m. javanica in a series of four separate greenhouse tests. after 62-64 days, or 1,032-1,072 degree days (10 c base), several of the crops evaluated showed moderate to severe levels of galling (> 3.0 on 0-5 scale) and moderate numbers of egg masses (>2.0 on 0-5 scale) in response to each of the nematode species and races. among the plants tested, collard ...	1995	19277321
comparative analysis of the mir319a microrna locus in arabidopsis and related brassicaceae.	micrornas (mirnas) are important regulators of gene expression in multicellular organisms. yet, little is known about their molecular evolution. the 20- to 22-nt long mirnas are processed in plants from foldbacks that are a few hundred base pairs in size. often, these foldbacks are embedded in much larger precursor transcripts. to investigate functional constraints on sequence evolution of mirna precursor genes, we have studied sequence variation in the precursor of mir319a, mir319a, between spe ...	2008	18296705
mineral content of traditional leafy vegetables from western kenya.	socio-economic changes that have taken place in africa have influenced people's eating habits in both rural and urban set-ups. most people prefer introduced foods to traditional foods, including plant foods whose consumption is widely regarded as a primitive culture manifesting poor lifestyles. however, recent studies on traditional plant foods have shown that some are highly nutritious; containing high levels of both vitamins and minerals. they also have potential as a remedy to counter food in ...	2007	17852510
estimation of genetic diversity among turkish kale populations (brassica oleracea var. acephala l.) using rapd markers.	20 populations of kale (b. oleracea var. acephala l.) selected from 127 populations for fresh consumption terms of yield and leaf quality characteristics as superior types using weight-based ranking method from the black sea region of turkey were evaluated at the dna level using randomly amplified polymorphic dna (rapd) markers compared to some morphological characters. the 7 primers selected from 100 decamers used generated 110 bands, of which 60 (54.5%) were polymorphic. jaccard's genetic dist ...	2007	17555128
a multi-element solid-phase extraction method for trace metals determination in environmental samples on amberlite xad-2000.	a method for the preconcentration of some transition elements at trace level was proposed using a column filled with amberlite xad-2000 resin. metal ions were adsorbed on xad-2000 as their diethyldithiocarbamate chelates, then analytes retained on the resin were eluted by 1 mol l(-1) nitric acid in acetone and determined by flame atomic absorption spectrometry (faas). the influences of some analytical parameters including ph of sample solution, ligand amount, the type, concentration and volume o ...	2007	17222968
[optimization of srap & issr technology and its application in the identification of seeds of brassica oleracea l].	in this study, the molecular marker technology of srap and issr were applied in rapid identification of seeds from eight species of brassica oleracea l. firstly, using the genomic dna of cabbage as template, srap and issr reaction systems were optimized through testing every factor, respectively, that affects pcr amplification. then, using the optimized reaction systems, 30 srap primer pairs and 15 issr primers were applied to amplify genomic dna of cabbage, savoy, purple cabbage, borecole, caul ...	2006	16894905
transgenic tobacco plants expressing bors1 gene from brassica oleracea var. acephala show enhanced tolerance to water stress.	water stress is by far the leading environmental stress limiting crop yields worldwide. genetic engineering techniques hold great promise for developing crop cultivars with high tolerance to water stress. in this study, the brassica oleracea var. acephala bors1 gene was transferred into tobacco through agrobacterium-mediated leaf disc transformation. the transgenic status and transgene expression of the transgenic plants was confirmed by polymerase chain reaction (pcr) analysis, southern hybridi ...	2005	16388139
[the study of transformation of tobacco with the stress responsible gene bors1 from brassica oleracea var. acephala].	transgenic tobacco plants expressing a stress responsive gene bors1, isolated from brassica oleracea var. acephala, under the control of the 35s promoter of the cauliflower mosaic virus were produced. some plants were further used to test the effect of high level bors1 expression on drought stress resistance. the presence of transgene in putative transgenic plants was confirmed by pcr analysis. thirty-six among 130 transformants showed amplification of predicted fragment of bors1 while no amplif ...	2005	16108381
dna-based identification of brassica vegetable species for the juice industry.	since kale (brassica oleracea var. acephala), a cruciferous vegetable with a high level of vitamins and functional compounds beneficial to health and wellness, has become widely used in the juice industry, a precise method for quality control of vegetable species is necessary. we describe here a dna-based identification method to distinguish kale from cabbage (brassica oleracea var. capitata), a closely related species, which can be inadvertently mixed with kale during the manufacturing process. ...	2003	14703312
parasitic and bacterial contamination in collards using effluent from treated domestic wastewater in chiang mai, thailand.	thailand often has inadequate water supply for agriculture during the dry season. the reuse of treated wastewater treatment plants could solve this problem. treatment of domestic wastewater of chiang mai municipality by the aerated lagoon system (al) releases more than 25,000 m3 of treated water everyday. the reuse of wastewater in agriculture is an efficient use of water, especially in tropical countries or in drought zones. the objective of this study is to demonstrate the possibility of using ...	2001	12041598
the in vivo effect of a brassica oleracea var. capitata extract on ehrlich ascites tumors of mus musculus balb/c mice.	an extract of brassica oleracea var. capitata juice was prepared using petroleum ether, ether, ethanol and an al2o3 column. the healing and tumor protecting effects of this extract were tested on ehrlich ascites (ea) solid tumors of mus musculus balb/c mice. complete disappearance of the tumors was observed in 54.5% of the animals in the experimental group (n = 22) which received 20 mg/day of the extract i.p. for 28 days. regression of the tumors (27%), fixation of tumor size (4%) and an increas ...	1999	10707127
expression of a self-incompatibility gene in a self-compatible line of brassica oleracea.	in cruciferous plants, self-pollination is prevented by the action of genes situated at the self-incompatibility locus or s-locus. the self-incompatibility reaction is associated with expression of stigma glycoproteins encoded by the s-locus glycoprotein (slg) gene. only a few cases of self-compatible plants derived from self-incompatible lines in the crucifer brassica have been reported. in these cases, self-compatibility was generally ascribed to the action of single genes unlinked to the s-lo ...	1993	8439745
the effect of harvest date on the domd, crude protein, smco and scn contents of the lamina, petiole and stem of fodder kale.		1983	6865339