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transcriptome profiling of pumpkin (cucurbita moschata duch.) leaves infected with powdery mildew.cucurbit powdery mildew (pm) is one of the most severe fungal diseases, but the molecular mechanisms underlying pm resistance remain largely unknown, especially in pumpkin (cucurbita moschata duch.). the goal of this study was to identify gene expression differences in pm-treated plants (harvested at 24 h and 48 h after inoculation) and untreated (control) plants of inbred line "112-2" using rna sequencing (rna-seq). the inbred line "112-2" has been purified over 8 consecutive generations of sel ...201829320569
salinity tolerance of grafted watermelon seedlings.in order to evaluate the salinity tolerance of grafted watermelon, two sets of experiments were conducted in a growing chamber where 'esmeralda' varieties were grafted onto interspecific squash (cucurbita maxima duch. × cucurbita moschata duch.) and lagenaria siceraria rootstocks. both non-grafted and self-garfted plants were used for control. for salt stress, 2.85 and 4.28 mm/l substrate doses of nacl were added with each irrigation in 2 day intervals for a duration of 23 days. interspecific-gr ...201729262705
dehydrodiconiferyl alcohol promotes bmp-2-induced osteoblastogenesis through its agonistic effects on estrogen receptor.estrogen deficiency results in an imbalance between the levels of bone-resorping osteoclasts and bone-forming osteoblasts, eventually leading to overall bone loss. dehydrodiconiferyl alcohol (dhca), a lignan compound originally isolated from cucurbita moschata, has been shown to bind to estrogen receptor, and indeed exhibits various activities of estrogen, such as anti-inflammatory and anti-oxidative stress effects. in this study, we tested whether synthetic dhca could affect the bmp-2-induced o ...201829253565
ectopic expression of pumpkin nac transcription factor cmnac1 improves multiple abiotic stress tolerance in arabidopsis.drought, cold and salinity are the major environmental stresses that limit agricultural productivity. nac transcription factors regulate the stress response in plants. pumpkin (cucurbita moschata) is an important cucurbit vegetable crop and it has strong resistance to abiotic stress; however, the biological functions of stress-related nac genes in this crop are largely unknown. this study reports the function of cmnac1, a stress-responsive pumpkin nac domain protein. the cmnac1-gfp fusion protei ...201729234347
evaluation of the impact of food matrix change on the in vitro bioaccessibility of carotenoids in pumpkin (cucurbita moschata) slices during two drying processes.the food matrix is a limiting factor in determining the bioaccessibility of carotenoids. the impact of food matrix change on the bioaccessibility of carotenoids during drying processes is still unknown. the effect of intermittent microwave vacuum-assisted drying (imvd) and hot air drying (had) on the in vitro liberation and micellization of carotenoids in pumpkin slices was studied. this variable depended on the changes of the matrix driven by the drying process. different changes in the cell mo ...201729165479
can highly cited herbs in ancient traditional chinese medicine formulas and modern publications predict therapeutic targets for diabetes mellitus?the prevalence of diabetes among all age groups worldwide was estimated to be more than 382 million in 2013. traditional chinese medicine (tcm) has been practiced for thousands of years, and substantial valuable experience and prescriptions have been accumulated in the tcm system for the treatment of diabetes. in recent decades, a large amount of experimental and clinical data has been published on the use of herbal medicines related to these ancient tcm prescriptions.201829102765
a high-density linkage map and qtl mapping of fruit-related traits in pumpkin (cucurbita moschata duch.).pumpkin (cucurbita moschata) is an economically worldwide crop. few quantitative trait loci (qtls) were reported previously due to the lack of genomic and genetic resources. in this study, a high-density linkage map of c. moschata was structured by double-digest restriction site-associated dna sequencing, using 200 f2 individuals of cmo-1 × cmo-97. by filtering 74,899 snps, a total of 3,470 high quality snp markers were assigned to the map spanning a total genetic distance of 3087.03 cm on 20 li ...201728986571
evaluation of three pumpkin species: correlation with physicochemical, antioxidant properties and classification using spme-gc-ms and e-nose methods.to ascertain the most discriminant variables for three pumpkin species principal component analysis (pca) was performed. twenty-four parameters (ph, conductivity, sucrose, glucose, total soluble solids, l*, a*, b*, individual weight, edible rate, firmness, citric acid, fumaric acid, l-ascorbic acid, malic acid, ppo activity, pod activity, total flavonoids, vitamin e, total phenolics, dpph, frap, β-carotene, and aroma) were considered. the studied pumpkin species were cucurbita maxima, cucurbita ...201728974797
karyotype stability and unbiased fractionation in the paleo-allotetraploid cucurbita genomes.the cucurbita genus contains several economically important species in the cucurbitaceae family. here, we report high-quality genome sequences of c. maxima and c. moschata and provide evidence supporting an allotetraploidization event in cucurbita. we are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the benincaseae tribe. we estimate that the two diploid progenitors successively diverged from benincaseae ...201728917590
evaluation of phytochemicals, antioxidant and burn wound healing activities of cucurbita moschata duchesne fruit peel.cucurbita moschata duchesne (pumpkin) is a well-known plant with several pharmacological effects. the aim of the present study was to assess burn wound healing activity of c. moschata peel extract (ce). also, standardized ce was assessed for antioxidant activity and antibacterial effects against major pathogens of burns.201728852445
amino acid profiling and antimicrobial activity of cucurbita moschata and lagenaria siceraria seed protein hydrolysates.cucurbita moschata and lagenaria siceraria seed proteins were extracted and hydrolysed with trypsin in order to recover antibacterial peptides. amino acid content and molecular weight distribution were estimated to justify their co-relationship with antimicrobial activity. antimicrobial activities of c. moschata and l. siceraria seed protein hydrolysates against three gram-negative bacteria and two gram-positive bacteria were evaluated. seed protein hydrolysates of both of these plants have sign ...201728783965
cytoprotective effects of pumpkin (cucurbita moschata) fruit extract against oxidative stress and carbonyl stress.background diabetes mellitus is a chronic endocrine disorder that is associated with significant mortality and morbidity due to microvascular and macrovascular complications. diabetes complications accompanied with oxidative stress and carbonyl stress in different organs of human body because of the increased generation of free radicals and impaired antioxidant defense systems. in the meantime, reactive oxygen species (ros) and reactive carbonyl species (rcs) have key mediatory roles in the deve ...028586926
a green analytical method for benzoyl peroxide determination by a sequential injection spectrophotometry using natural reagent extracts from pumpkin.a green and environmental friendly method with simple sequential injection spectrophotometry using natural reagent extract from pumpkin (cucurbita moschata decne) for determination of benzoyl peroxide (bpo) was developed. the maximum absorption of the yellow crude extract containing the β-carotene compound occurred at a wavelength of 450nm. the yellow extract solution was bleached by bpo to change the color to a colorless solution. the optimum conditions of the proposed method, such as extractio ...201728551134
phytochemicals and antioxidative enzymes defence mechanism on occurrence of yellow vein mosaic disease of pumpkin (cucurbita moschata).pumpkin (cucurbita moschata) samples showing yellow vein mosaic disease in varanasi region were identified with begomovirus infection using pcr amplification. a sequencing analysis of the full genome revealed that it is a strain of tomato leaf curl palampur virus (genbank id. fj931537). phytochemical composition and antioxidative enzyme levels were compared in infected and healthy plants. the study revealed that the amount of total protein declined in the infected leaves but elevated up to 135 % ...201328324587
plant exudates trigger leaf-trenching by cabbage loopers, trichoplusia ni (noctuidae).cabbage loopers, trichoplusia ni, cut a narrow trench across leaves of plants that release exudate, then feed distal to the trench in an area of reduced exudation. the larvae do not normally trench plant species such as plantain, plantago lanceolata, that lack exudate. to determine what cues elicit trenching, i reared larvae to the final instar on plantain, then applied test solutions to their mouthparts during feeding. loopers that received latex from lactuca serriola (asteraceae) or phloem exu ...199728307485
α-carotene and β-carotene content in raw and cooked pulp of three mature stage winter squash "type butternut".winter squash "type butternut" is harvested in physiological ripening for better commercial distribution, when sensory and/or nutritional quality is not optimum for consumption. the objective of this study was to quantify the content of α-carotene, β-carotene, color and dry matter in the pulp of raw and microwave-cooked winter squash "type butternut" (variety cosmof1) in three states of commercial maturity. immature, mature, and very mature fruit, defined at the time of the harvest by the percen ...201528231218
cultivar-based introgression mapping reveals wild species-derived pm-0, the major powdery mildew resistance locus in squash.powdery mildew is a major fungal disease on squash and pumpkin (cucurbita spp.) in the us and throughout the world. genetic resistance to the disease is not known to occur naturally within cucurbita pepo and only infrequently in cucurbita moschata, but has been achieved in both species through the introgression of a major resistance gene from the wild species cucurbita okeechobeensis subsp. martinezii. at present, this gene, pm-0, is used extensively in breeding, and is found in nearly all powde ...201627936008
isolation and functional characterization of cslsi1, a silicon transporter gene in cucumis sativus.cucumber (cucumis sativus) is a widely grown cucurbitaceous vegetable that exhibits a relatively high capacity for silicon (si) accumulation, but the molecular mechanism for silicon uptake remains to be clarified. here we isolated and characterized cslsi1, a gene encoding a silicon transporter in cucumber (cv. mch-4). cslsi1 shares 55.70 and 90.63% homology with the lsi1s of a monocot and dicot, rice (oryza sativa) and pumpkin (cucurbita moschata), respectively. cslsi1 was predominantly expresse ...201727701737
carboxylesterase-involved metabolism of di-n-butyl phthalate in pumpkin (cucurbita moschata) seedlings.uptake and accumulation by plants is a significant pathway in the migration and transformation of phthalate esters (paes) in the environment. however, limited information is available on the mechanisms of pae metabolism in plants. here, we investigated the metabolism of di-n-butyl phthalate (dnbp), one of the most frequently detected paes, in pumpkin (cucurbita moschata) seedlings via a series of hydroponic experiments with an initial concentration of 10 mg l(-1). dnbp hydrolysis occurred primar ...201727697378
can adverse effects of acidity and aluminum toxicity be alleviated by appropriate rootstock selection in cucumber?low-ph and aluminum (al) stresses are the major constraints that limit crop yield in acidic soils. grafting vegetable elite cultivars onto appropriate rootstocks may represent an effective tool to improve crop tolerance to acidity and al toxicity. two greenhouse hydroponic experiments were performed to evaluate growth, yield, biomass production, chlorophyll index, electrolyte leakage, mineral composition, and assimilate partitioning in plant tissues of cucumber plants (cucumis sativus l. "ekron" ...201627621740
combined infrared-vacuum drying of pumpkin slices.infrared-vacuum dehydration characteristics of pumpkin (cucurbita moschata) were evaluated in a combined dryer system. the effects of drying parameters, infrared radiation power (204-272 w), system pressure (5-15 kpa), slice thickness (5 and 7 mm) and time (0-220 min) on the drying kinetics and characteristics of pumpkin slices were investigated. the vacuum pressure, lamp power and slice had significant effect on the drying kinetics and various qualities of the dried pumpkin. moisture ratios wer ...201627407204
cucurbita moschata duch. and its active component, β-carotene effectively promote the immune responses through the activation of splenocytes and macrophages.cucurbita moschata duch. has long been used for traditional health food in many countries. however, to enhance the immune system of cucurbita moschata duch. and its major component, β-carotene is not clear. here, we determined the immune enhancement effect of cucurbita moschata duch. and β-carotene in mouse splenocytes and raw 264.7 macrophage cell line. we prepared baked cucurbita moschata duch. (sweetme sweet pumpkin(tm), ssp) and steamed cucurbita moschata duch. (sc). splenocytes isolated fro ...201627315229
biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of cucurbita moschata that accumulate recombinant bacterial lina.γ-hch was successfully degraded using lina-expressed transgenic hairy root cultures of cucurbita moschata . fusing an endoplasmic reticulum-targeting signal peptide to lina was essential for stable accumulation in the hairy roots. the pesticide γ-hexachlorocyclohexane (γ-hch) is a persistent organic pollutant (pop) that raises public health and environmental pollution concerns worldwide. although several isolates of γ-hch-degrading bacteria are available, inoculating them directly into γ-hch-con ...201627295266
performance of the striped mealybug ferrisia virgata cockerell (hemiptera: pseudococcidae) under variable conditions of temperature and mating.mealybugs have strong associations with their host plants due to their limitations for dispersal. thus, environmental conditions and host quality may impact the biological traits of mealybugs. to the best of our knowledge, we are the first to report on the biology of a brazilian population of the striped mealybug ferrisia virgata cockerell (hemiptera: pseudococcidae), which has recently been reported to infest cotton in brazil. we evaluated the development and reproductive performance of f. virg ...201427193398
improvement of depressive behavior by sweetme sweet pumpkin™ and its active compound, β-carotene.sweetme sweet pumpkin™ (ssp, baked cucurbita moschata duch.) has been used to treat patients with depression in korea. however, the role of ssp in improving depression has not been elucidated yet. thus, we assessed the antidepressant-like effect of ssp and its active compound, β-carotene, with the forced swimming test (fst).201626820672
sequence variations in the fad2 gene in seeded pumpkins.seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. the fatty acid desaturase-2 (fad2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. however, little is known about sequence variations in fad2 in seeded pumpkins. twenty-seven fad2 clones from 27 accessions of cucurbita moschata, cucurbita maxima, cucurbita pepo, and cucurbita ficifoli ...201526782391
α-cyclodextrin encapsulation of supercritical co₂ extracted oleoresins from different plant matrices: a stability study.here we describe the encapsulation in α-cyclodextrins (α-cds) of wheat bran, pumpkin and tomato oleoresins, extracted by supercritical carbon dioxide, to obtain freeze-dried powders useful as ready-to-mix ingredients for novel functional food formulation. the stability of tocochromanols, carotenoids and fatty acids in the oleoresin/α-cd complexes, compared to the corresponding free oleoresins, was also monitored over time in different combinations of storage conditions. regardless of light, stor ...201626776025
effect of different drying methods on moisture ratio and rehydration of pumpkin slices.this study was carried to determine the influence of hot air drying process and combined methods on physicochemical properties of pumpkin (cucurbita moschata) samples. the experiments in hot air chamber were lead at 50, 60 and 70 °c. the combined method consists of a triple combination of the main drying techniques. thus, in first stage the samples were dried in hot air convection at 60 °c followed by hot air ventilation at 40 °c simultaneous with microwave. the time required to reduce the moist ...201626575719
dehydrodiconiferyl alcohol (dhca) modulates the differentiation of th17 and th1 cells and suppresses experimental autoimmune encephalomyelitis.dehydrodiconiferyl alcohol (dhca), originally isolated from the stems of cucurbita moschata, has previously been shown to exhibit anti-adipogenic and anti-lipogenic effects in 3t3-l1 cells and primary mouse embryonic fibroblasts (mefs) (lee et al., 2012). here, we investigated whether synthetic dhca could suppress the cd4 t helper 17 (th17)-mediated production of the interleukin (il)-17 protein. the results from rt-qpcr suggest that dhca-mediated down-regulation of il-17 occurred at the transcri ...201526477735
biology of anastrepha grandis (diptera: tephritidae) in different cucurbits.anastrepha grandis (macquart) (diptera: tephritidae) is one of the main pests of cucurbits in brazil. losses occur due to the damage caused to the fruits and the embargo on exports, as a. grandis is considered a quarantine pest in countries that import brazilian cucurbits. this study aimed to evaluate the development of a. grandis in hosts of the cucurbitaceae family. the hosts used were stem squash (cucurbita pepo l.), squash (cucurbita moschata duchesne), chayote [sechium edule (jacq.) swartz] ...201526470226
nutritional evaluation of phosphorylated pumpkin seed (cucurbita moschata) protein concentrate in silver catfish rhamdia quelen (quoy and gaimard, 1824).an 8-week feeding trial was conducted to evaluate the effect of replacing fish meal with pumpkin seed meal (psm) or phosphorylated protein concentrate of pumpkin seed meal (ppcps) on growth and metabolic responses of silver catfish. five isonitrogenous and isocaloric diets were formulated. control diet contained fish meal as the main protein source. the treatment groups contained 25 and 50% of either psm or ppcps protein replaced the fishmeal protein. a total of 400 silver catfish, with initial ...201526377938
subcellular distribution and uptake mechanism of di-n-butyl phthalate in roots of pumpkin (cucurbita moschata) seedlings.phthalate acid esters (paes) are of particular concern due to their potential environmental risk to human and nonhuman organisms. although uptake of paes by plants has been reported by several researchers, information about the intracellular distribution and uptake mechanisms of paes is still lacking. in this study, a series of hydroponic experiments using intact pumpkin (cucurbita moschata) seedlings was conducted to investigate how di-n-butyl phthalate (dnbp), one of the most frequently identi ...201626304812
a novel aqueous two phase system composed of surfactant and xylitol for the purification of lipase from pumpkin (cucurbita moschata) seeds and recycling of phase components.lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. a novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from cucurbita moschata. the influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investiga ...201526091076
hplc-dad-ms(n) characterisation of carotenoids from apricots and pumpkins for the evaluation of fruit product authenticity.carotenoids including carotenoid esters from six apricot (prunus armeniaca l.) cultivars and from eight cultivars from three pumpkin species (cucurbita maxima duch., cucurbita pepo l., and cucurbita moschata duch.) were extracted without saponification, separated on a c-30 reversed-phase column and characterised by high-performance liquid chromatography/atmospheric pressure chemical ionisation-mass spectrometry (lc-ms). the predominant free carotenoids were quantified by hplc with diode array de ...200826049248
high throughput sequencing of small rnas in the two cucurbita germplasm with different sodium accumulation patterns identifies novel micrornas involved in salt stress response.micrornas (mirnas), a class of small non-coding rnas, recognize their mrna targets based on perfect sequence complementarity. mirnas lead to broader changes in gene expression after plants are exposed to stress. high-throughput sequencing is an effective method to identify and profile small rna populations in non-model plants under salt stresses, significantly improving our knowledge regarding mirna functions in salt tolerance. cucurbits are sensitive to soil salinity, and the cucurbita genus is ...201526010449
effective suppression of pro-inflammatory molecules by dhca via ikk-nf-κb pathway, in vitro and in vivo.dehydrodiconiferyl alcohol (dhca), a lignan compound isolated from cucurbita moschata, has previously been shown to contain anti-adipogenic and antilipogenic effects on 3t3-l1 cells and mouse embryonic fibroblasts. as some of phytochemicals derived from natural plants show anti-inflammatory or antioxidative activities, we determined whether dhca affects the production of pro-inflammatory mediators and also investigated its underlying mechanisms.201525802070
effects of grafting with pumpkin rootstock on carbohydrate metabolism in cucumber seedlings under ca(no3)2 stress.this study investigated the effects of grafting on the carbohydrate status and the enzymes of carbohydrate metabolism in self-grafted and grafted cucumber seedlings using the salt-tolerant pumpkin rootstock 'qingzhen 1' (cucurbita maxima × cucurbita moschata) under 80 mm ca(no3)2 stress for 6 d. the growth of self-grafted seedlings was significantly inhibited after the treatment of ca(no3)2 stress, whereas the inhibition of growth was alleviated in pumpkin rootstock-grafted seedlings. ca(no3)2 s ...201525579659
instrumental and sensory analyses of quality attributes of grafted specialty melons.soilborne disease management remains a great challenge in melon production with the phaseout of soil fumigant methyl bromide. grafting has been shown to be an effective approach to control soilborne diseases. however, previous research has yielded mixed results regarding the impacts of rootstock on fruit quality. very few studies have assessed melon quality attributes using both sensory evaluation and instrumental methods.201525512001
cucumber (cucumis sativus l.) and kabocha squash (cucurbita moschata duch).we established improved methods for agrobacterium-mediated transformation of cucumber (cucumis sativus l.) and kabocha squash (cucurbita moschata duch). vacuum infiltration of cotyledonary explants with agrobacterium suspension enhanced the agrobacterium infection efficiency in the proximal regions of explants. wounding treatment was also essential for kabocha squash. cocultivation on filter paper wicks suppressed necrosis of explants, keeping regeneration efficacy. putative transgenic plants we ...201525300850
occurrence of squash yellow mild mottle virus and pepper golden mosaic virus in potential new hosts in costa rica.leaf samples of solanum lycopersicum, capsicum annuum, cucurbita moschata, cucurbita pepo, sechium edule and erythrina spp. were collected. all samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. a sequence of ∼1,100 bp was obtained from the genomic component dna-a of 14 samples. in addition, one sequence of ∼580 bp corresponding to the coat protein (av1) was obtained from a chayote (s. edule) leaf sample. the presence of squash yellow mild mottle viru ...201325288955
upregulation of heme oxygenase-1 expression by dehydrodiconiferyl alcohol (dhca) through the ampk-nrf2 dependent pathway.oxidative stress is induced by the accumulation of free radicals, resulting in an imbalanced cellular redox state, which has been implicated in a variety of human diseases. dehydrodiconiferyl alcohol (dhca), a lignan compound isolated from cucurbita moschata, has previously been reported to contain anti-adipogenic and anti-lipogenic effects on 3t3-l1 cells and primary mefs (abraham and kappas, 2008). in this study, it was tested whether dhca could affect the expression of ho-1, using raw264.7 mo ...201425261737
ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (cucurbita moschata).an efficient ultrasound-assisted enzymatic extraction (uaee) of cucurbita moschata polysaccharides (cmcp) was established and the cmcp antioxidant activities were studied. the uaee operating parameters (extraction temperature, ultrasonic power, ph, and liquid-to-material ratio) were optimized using the central composite design (ccd) and the mass transfer kinetic study in uaee procedure was used to select the optimal extraction time. enzymolysis and ultrasonication that were simultaneously conduc ...201425256490
changes in carotenoids during processing and storage of pumpkin puree.changes in the contents of carotenoids and their true retentions (% tr) during the production of puree of cucurbita moschata 'menina brasileira' and of cucurbita maxima 'exposição' pumpkins and the stability of such compounds during 180days of storage were monitored by liquid chromatography coupled with a photodiode array detector. cooking caused higher losses than commercial sterilisation. high losses of xanthophylls such as lutein and violaxanthin were noted during processing and storage of pu ...201125214348
effects of season and storage period on accumulation of individual carotenoids in pumpkin flesh (cucurbita moschata).carotenoids are antioxidants with pharmaceutical potential. the major carotenoids important to humans are α-carotene, β-carotene, lycopene, lutein, zeaxanthin, and β-cryptoxanthin. some of the biological functions and actions of these individual carotenoids are quite similar to each other, whereas others are specific. besides genotype and location, other environmental effects such as temperature, light, mineral uptake, and ph have been found affect carotenoid development in plant tissues and org ...201425007748
effects of root applications of gibberellic acid on photosynthesis and growth in c3 and c 4 plants.the effects of root applications of gibberellic acid (ga3) on growth and photosynthesis of 12 species of plants including c3 monocots (triticum aestivum l., wheat, hordeum vulgare l., barley and avena sativa l., oat), c3 dicots (vigna radiata l., mung bean, cucurbita moschata l., squash and capsicum annuum l., pepper), c4 monocots (zea mays l., corn, sorghum vulgare l., sorghum and panicum ramosum l., millet) and c4 dicots (amaranthus retroflexus l., pigweed, kochia scoparis l., kochia and gomph ...198524442874
antioxidant enzymatic activities and gene expression associated with heat tolerance in the stems and roots of two cucurbit species ("cucurbita maxima" and "cucurbita moschata") and their interspecific inbred line "maxchata".the elucidation of heat tolerance mechanisms is required to combat the challenges of global warming. this study aimed to determine the antioxidant enzyme responses to heat stress, at the enzymatic activity and gene expression levels, and to investigate the antioxidative alterations associated with heat tolerance in the stems and roots of squashes using three genotypes differing in heat tolerance. plants of heat-tolerant "c. moschata", thermolabile "c. maxima" and moderately heat-tolerant intersp ...201324336062
hypolipidemic effect of the polysaccharides extracted from pumpkin by cellulase-assisted method on mice.the fruit of pumpkin (cucurbita moschata) is one of the most important vegetables in the world. this study was conducted to investigate the hypolipidemic effect of the polysaccharide isolated from pumpkin (pp). male sprague-dawley rats were divided into three groups and treated with diets containing either high fat, pp, or normal fat. oral administration of pp could significantly decrease the levels of plasma triacylglycerol (tg), total cholesterol (tc), and plasma low-density lipoprotein choles ...201424325857
d-amino-acid-stimulated ethylene production in seed tissues.ethylene production by axial and cotyledonary tissues excised from xanthium pennsylvanicum wallr. seeds was markedly (up to 5-fold) stimulated by the d-isomers of phenylalanine, valine, leucine, threonine, methionine and eithionine while the l-isomers caused no such effect. responsiveness of these seed tissues to d-methionine appeared soon after the beginning of imbibition, reached a maximum after 6-12 and 12-24 h for the axial and cotyledonary tissues, respectively, and then decreased sharply. ...198024306194
cellulase-assisted extraction of polysaccharides from cucurbita moschata and their antibacterial activity.in this study, cellulase-assisted extraction of water soluble polysaccharides from pumpkin (cucurbita moschata) and their antibacterial activity were investigated. the polysaccharides yield was monitored during the extraction process. the optimum extraction conditions were determined as follows: time, 40 min; temperature, 55°c; ph, 4.5; and cellulase amount, 4,000 u/g. the extracts were centrifuged, filtered, proteins removed by sevag method, concentrated to approximately 15% (w/v), precipitated ...201424299793
grafting-responsive mirnas in cucumber and pumpkin seedlings identified by high-throughput sequencing at whole genome level.grafting is an important agricultural technique widely used for improving growth, yields and tolerance of crops to abiotic and biotic stresses. as one type of endogenous, non-coding small rnas, micrornas (mirnas) regulate development and responsiveness to biotic and abiotic stresses by negatively mediating expression of target genes at the post-transcriptional level. however, there have been few detailed studies to evaluate the role of mirnas in mediation of grafting-induced physiological proces ...201424279842
characteristics of organic acids in the fruit of different pumpkin species.the aim of the research was to determine the composition of organic acids in fruit of different cultivars of three pumpkin species. the amount of acids immediately after fruit harvest and after 3 months of storage was compared. the content of organic acids in the examined pumpkin cultivars was assayed using the method of high performance liquid chromatography (hplc). three organic acids (citric acid, malic acid, and fumaric acid) were identified in the cultivars, whose content considerably varie ...201424262577
effect of drying and co-matrix addition on the yield and quality of supercritical co₂ extracted pumpkin (cucurbita moschata duch.) oil.in this work a process for obtaining high vitamin e and carotenoid yields by supercritical carbon dioxide (sc-co₂) extraction from pumpkin (cucurbita moschata duch.) is described. the results show that the use of a vacuum oven-dried [residual moisture (∼8%)] and milled (70 mesh sieve) pumpkin flesh matrix increased sc-co₂ extraction yields of total vitamin e and carotenoids of ∼12.0- and ∼8.5-fold, respectively, with respect to the use of a freeze-dried and milled flesh matrix. the addition of m ...201424262563
cucurbita spp. and cucumis sativus enhance the dissipation of polychlorinated biphenyl congeners by stimulating soil microbial community development.a number of cucurbita species have the potential to extract polychlorinated biphenyls (pcbs) from soil, but their impact on the soil microbial communities responsible for pcb degradation remains unclear. a greenhouse experiment was conducted to investigate the effect of three cucurbita and one cucumis species on pcb dissipation and soil microbial community structure. compared to the unplanted control, enhanced losses of pcbs (19.5%-42.7%) were observed in all planted soils. cucurbita pepo and cu ...201424077568
[effects of host plants on the life table parameters of experimental populations of aphis gossypii].a comparative study was conducted on the life table parameters of aphis gossypii reared on five host plant species at (25 +/- 1) degrees c in laboratory. there existed significant differences in the durations of various developmental stages, adult longevity, mean offspring number per day, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time, and population doubling time for the a. gossypii populations reared on the host plants. for the aphids on lagena ...201324015567
effect of gamma irradiation and storage time on microbial growth and physicochemical characteristics of pumpkin (cucurbita moschata duchesne ex poiret) puree.the effect of gamma irradiation (0-2 kgy) and storage time (0-28 days) on microbial growth and physicochemical characteristics of a packed pumpkin puree was studied. for that purpose, a factorial design was applied. the puree contained potassium sorbate, glucose and vanillin was stored at 25°c . gamma irradiation diminished and storage time increased microbial growth. a synergistic effect between both variables on microbial growth was observed. storage time decreased ph and color of purees. sorb ...201423733817
tomato leaf curl new delhi virus is associated with pumpkin leaf curl: a new disease in northern india.during 2006, pumpkin leaf curl-a new disease was observed in the experimental field at indian agricultural research institute. the disease was characterized by upward leaf curl with chlorotic patches and stunting of plant. polymerase chain reaction (pcr) with coat protein specific primers to tomato leaf curl new delhi virus (tolcndv) indicated association of a begomovirus with the disease. the sequence comparison and phylogenetic analysis of the complete dna genome further revealed the identity ...201123730002
molecular characterization of tomato leaf curl palampur virus and pepper leaf curl betasatellite naturally infecting pumpkin (cucurbita moschata) in india.pumpkin cultivation in india is affected by severe incidence of a yellow vein mosaic disease. tomato leaf curl new delhi virus and squash leaf curl china virus are known to be associated with this disease in india. we were able to identify a third begomovirus-tomato leaf curl palampur virus (tolcpmv), from pumpkin showing typical symptoms of the disease at varanasi based on the sequence of complete dna-a genome of the virus. the complete dna-a sequence of the virus shared more than 99% sequence ...201123637491
chlorotic curly stunt: a severe begomovirus disease of bottle gourd in northern india.bottle gourd (lagenaria siceraria) an important vegetable crop in india was observed to be affected by a chlorotic curly stunt disease (ccsd) during 2003-2006 in the vegetable growing areas of delhi and adjoining state of haryana. the affected plants are severely stunted and bear very small chlorotic and mildly curled leaves. incidence of the disease varied from 4.7 to 36%. the disease could be easily transmitted by whitefly, bemisia tabaci but not by sap. the causal virus was found to be a bego ...201023637479
phloem long-distance delivery of flowering locus t (ft) to the apex.cucurbita moschata flowering locus t-like 2 (hereafter ftl2) and arabidopsis thaliana (arabidopsis) flowering locus t (ft), components of the plant florigenic signaling system, move long-distance through the phloem from source leaves to the vegetative apex where they mediate floral induction. the mechanisms involved in long-distance trafficking of ft/ftl2 remain to be elucidated. in this study, we identified the critical motifs on both ft and ftl2 required for cell-to-cell trafficking through mu ...201323607279
anti-hyperglycaemic globulins from selected cucurbitaceae seeds used as antidiabetic medicinal plants in africa.the recent epidemic of diabetes mellitus (dm) in africa, coupled with rampant poverty, is an indication of the urgent need to develop new efficacious, cheaper and more available drugs to face this growing public health challenge. a number of plants products among which the protein-rich cucurbitaceae seeds are commonly used in traditional medicine with increasing acclaimed efficacy against dm. the aim of this study was to analyse and evaluate the hypoglycaemic activity of storage proteins of five ...201323506532
microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon.microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ...201123431109
modeling and optimization of ultrasound-assisted extraction of polysaccharide from cucurbita moschata.polysaccharides from pumpkin were extracted by ultrasound-assisted extraction technology using four factors at five levels central composite rotatable response surface design (ccrd). on using single factor analysis, process variables such as extraction temperature (50-70 °c), power of ultrasound (50-70 w), time (15-25 min) and solid-liquid ratio (1:10-1:20 g/ml) were selected. experiments were conducted to evaluate the effects of four independent variables on the maximum extraction yield of poly ...201323399253
pumpkin (cucurbita moschata) fruit extract improves physical fatigue and exercise performance in mice.pumpkin (cucurbita moschata) is a popular and nutritious vegetable consumed worldwide. the overall purpose of this study was to evaluate the effects of c. moschata fruit extract (cme) on anti-fatigue and ergogenic functions following physiological challenges. male icr mice from four groups designated vehicle, cme-50, cme-100 and cme-250, respectively (n = 8 per group in each test) were orally administered cme for 14 days at 0, 50, 100 and 250 mg/kg/day. the anti-fatigue activity and exercise per ...201223047485
ethylene-induced overproduction of reactive oxygen species is responsible for the development of watersoaking in immature cucumber fruit.watersoaking is an ethylene-induced disorder observed in some members of the cucurbitaceae including cucumber (cucumis sativus l.), watermelon (citrullus lanatus thunb. matsum and nakai), and tropical pumpkin (cucurbita moschata duch.). previous studies have found that immature beit-alpha cucumber (cv. manar) exhibit watersoaking after 6d of continuous exposure to 10 μll(-1) ethylene in air (21 kpa o(2)). the present study was designed to investigate the early dynamics of ethylene responses in i ...201322999588
complete nucleotide sequence of watermelon chlorotic stunt virus originating from oman.watermelon chlorotic stunt virus (wmcsv) is a bipartite begomovirus (genus begomovirus, family geminiviridae) that causes economic losses to cucurbits, particularly watermelon, across the middle east and north africa. recently squash (cucurbita moschata) grown in an experimental field in oman was found to display symptoms such as leaf curling, yellowing and stunting, typical of a begomovirus infection. sequence analysis of the virus isolated from squash showed 97.6-99.9% nucleotide sequence iden ...201222852046
characterization of the small rna component of leaves and fruits from four different cucurbit species.micrornas (mirnas) are a class of non-coding small rnas involved in post-transcriptional regulation of gene expression critical for plant growth and development, stress responses and other diverse biological processes in plants. the cucurbitaceae or cucurbit family represents some of economically important species, particularly those with edible and medicinal fruits. genomic tools for the molecular analysis of members of this family are just emerging. partial draft genome sequence became availab ...201222823569
nutritional, sensory and physical analysis of pumpkin flour incorporated into weaning mix.the objective of this study was to develop a cereal-pulse complementary food fortified with different concentrations of pumpkin powder (cucurbita moschata), and to analyse its sensory and physic-chemical parameters. fresh pumpkins(cucurbita moschata) were procured from the market and dehydrated and powdered in the laboratory. sorghum (sorghum vulgare) and whole green gram (vigna radiate) were germinated, dried, pulverised and combined with powdered rice (oryza sativa) in the ratio of 2:1:1. pump ...201022691991
bioactive compounds in lipid fractions of pumpkin (cucurbita sp) seeds for use in food.seeds are considered to be agro-industrial residues, which can be used as source of macronutrients and/or raw material for extraction of vegetable oils, since they present great quantities of bioactive compounds. this study aimed to characterize the lipid fractions and the seeds of pumpkin (cucurbita sp) varieties nova caravela, mini paulista, menina brasileira, and moranga de mesa aiming at using them in food. the chemical composition of the seeds was performed according to the official methods ...201222671521
distinct genetic lineages of bactrocera caudata (insecta: tephritidae) revealed by coi and 16s dna sequences.the fruit fly bactrocera caudata is a pest species of economic importance in asia. its larvae feed on the flowers of cucurbitaceae such as cucurbita moschata. to-date it is distinguished from related species based on morphological characters. specimens of b. caudata from peninsular malaysia and indonesia (bali and lombok) were analysed using the partial dna sequences of cytochrome c oxidase subunit i (coi) and 16s rrna genes. both gene sequences revealed that b. caudata from peninsular malaysia ...201222615962
carotenoid accumulation and carotenogenic gene expression during fruit development in novel interspecific inbred squash lines and their parents.carotenoid levels and composition during squash fruit development were compared in cucurbita moschata , cucurbita maxima , and two lines of their interspecific inbred lines, namely, maxchata1 and maxchata2. eight genes associated with carotenoid biosynthesis were analyzed by quantitative rt-pcr. the two squash species and their interspecific inbred lines exhibited different qualitative and quantitative carotenoid profiles and regulatory mechanisms. c. moschata had the lowest total carotenoid con ...201222574777
dehydrodiconiferyl alcohol isolated from cucurbita moschata shows anti-adipogenic and anti-lipogenic effects in 3t3-l1 cells and primary mouse embryonic fibroblasts.a water-soluble extract from the stems of cucurbita moschata, code named pg105, was previously found to contain strong anti-obesity activities in a high fat diet-induced obesity mouse model. one of its biological characteristics is that it inhibits 3t3-l1 adipocytes differentiation. to isolate the biologically active compound(s), conventional solvent fractionation was performed, and the various fractions were tested for anti-adipogenic activity using oil red o staining method. a single spot on t ...201222262865
effect of reproductive mode on host plant utilization of melon aphid (hemiptera: aphididae).variation in the reproductive mode of melon aphid aphis gossypii glover occurred on the large geographic scale, but the performance of different reproductive modes to use host plant is poorly understood. life tables of melon aphid population that undergo the anholocyclic, androcyclic, and intermediate reproductive mode were conducted on different host plants. the results showed that the anholocyclic and androcyclic strains could become adults and produce offspring on cotton gossypium hirsutum l. ...201022182553
cucurmosin induces apoptosis of bxpc-3 human pancreatic cancer cells via inactivation of the egfr signaling pathway.pancreatic cancer remains the fourth most common cause of cancer-related death in the united states. potent therapeutic strategies are urgently needed for pancreatic cancer. cucurmosin is a novel type 1 ribosome-inactivating protein (rip) isolated from the sarcocarp of cucurbita moschata (pumpkin). due to its cytotoxicity, cucurmosin can inhibit tumor cell proliferation through induction of apoptosis on tumor cells, but the specific mec ...201122139427
olfactory cues from plants infected by powdery mildew guide foraging by a mycophagous ladybird beetle.powdery mildews (erysiphales) are economically important plant pathogens that attack many agricultural crops. conventional management strategies involving fungicide application face challenges, including the evolution of resistance and concerns over impacts on non-target organisms, that call for investigation of more sustainable alternatives. mycophagous ladybird beetles (coleoptera: coccinellidae) feed on powdery mildew and have considerable potential as biological control agents; however, the ...201121876772
lectins but not antifungal proteins exhibit anti-nematode activity.a variety of lectins and antifungal proteins were tested for toxicity against the plant parasitic nematodes ditylenchus dipsaci and heterodera glycines. it was found that lectins from the mushrooms xylaria hypoxylon, agrocybe cylindracea and tricholoma mongolicum (tml-1) were the most potent against d. dipsaci, with ec(50) being 4.7, 9, and 20mg/ml, respectively. lectins from pseudostellaria heterophylla, samta tomato, and the mushrooms t. mongolicum (tml-2), ganoderma lucidum, and boletus eduli ...200921784014
silicon efflux transporters isolated from two pumpkin cultivars contrasting in si uptake.the accumulation of silicon (si) differs greatly with plant species and cultivars due to different ability of the roots to take up si. in si accumulating plants such as rice, barley and maize, si uptake is mediated by the influx (lsi1) and efflux (lsi2) transporters. here we report isolation and functional analysis of two si efflux transporters (cmlsi2-1 and cmlsi2-2) from two pumpkin (cucurbita moschata duch.) cultivars contrasting in si uptake. these cultivars are used for rootstocks of bloom ...201121617377
genes for resistance to zucchini yellow mosaic in tropical pumpkin.four cultigens of cucurbita moschata resistant to zucchini yellow mosaic virus were crossed with the susceptible 'waltham butternut' and with each other in order to clarify the mode of inheritance of resistance and relationships among the genes involved. five loci were segregating, with genes for resistance zym-0 and zym-4 carried by 'nigerian local' and one of them also carried by 'nicklow's delight,' zym-1 carried by 'menina,' and zym-6 carried by 'soler.' a recessive gene carried by 'waltham ...201121493595
agrobacterium-mediated transformation of kabocha squash (cucurbita moschata duch) induced by wounding with aluminum borate whiskers.an efficient genetic transformation method for kabocha squash (cucurbita moschata duch cv. heiankogiku) was established by wounding cotyledonary node explants with aluminum borate whiskers prior to inoculation with agrobacterium. adventitious shoots were induced from only the proximal regions of the cotyledonary nodes and were most efficiently induced on murashige-skoog agar medium with 1 mg/l benzyladenine. vortexing with 1% (w/v) aluminum borate whiskers significantly increased agrobacterium i ...201121400224
glucose-lowering activity of novel tetrasaccharide glyceroglycolipids from the fruits of cucurbita moschata.two new tetrasaccharide glyceroglycolipids were obtained from pumpkin. the structures of the two compounds were determined using chemical methods and spectroscopic analysis. both compounds demonstrated significant glucose-lowering effects in streptozotocin- and high-fat-diet-induced diabetic mice.201021215628
isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation.a high accumulation of silicon (si) is required for overcoming abiotic and biotic stresses, but the molecular mechanisms of si uptake, especially in dicotyledonous species, is poorly understood. herein, we report the identification of an influx transporter of si in two cucurbita moschata (pumpkin) cultivars greatly differing in si accumulation, which are used for the rootstocks of bloom and bloomless cucumis sativus (cucumber), respectively. heterogeneous expression in both xenopus oocytes and r ...201121205032
potential mechanisms of hypocholesterolaemic effect of thai spices/dietary extracts.several thai spices/dietary ingredients were previously shown to have hypocholesterolaemic effects. these studies were mostly conducted in animal models in which the mechanisms of action were not yet well-established. therefore, this study aimed to investigate the potential mechanism of hypocholesterolaemic action of 12 selected plants, namely hibiscus sabdariffa l., moringa oleifera lam., cucurbita moschata duchesne, ananas comosus (l.) merr., zingiber officinale, morus alba l., camellia sinens ...201120623425
[entomophilic pollination of squash, cucurbita moschata (cucurbitaceae)].the objectives of this work were to determine the squash entomofauna in the region of viçosa, minas gerais state, to study their behavior on flowers and their importance for pollination, verifying the role of each pollinator. the most common species were trigona spinipes (fabricius), trigona hyalinata (lepeletier), apis mellifera (l.) and melipona quadrifasciata (lepeletier). the visitation behavior of a. mellifera, m. quadrifasciata, and bombus morio (swederus) were similar. they visited flower ...201020498949
new phenolic glycosides from the seeds of cucurbita moschata.two new phenolic glycosides were isolated from the seeds of cucurbita moschata. their structures were elucidated as (2-hydroxy)phenylcarbinyl 5-o-benzoyl-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (1) and 4-beta-d-(glucopyranosyl hydroxymethyl)phenyl 5-o-benzoyl-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (2) on the basis of spectroscopic analysis and chemical evidence.200920183300
characterization of acid-extracted pectin-enriched products obtained from red beet (beta vulgaris l. var. conditiva) and butternut (cucurbita moschata duch ex poiret).chemical and rheological characteristics of fractions enriched in soluble dietary fiber are reported. these fractions were obtained through acid hydrolysis of butternut (cucurbita moschata duch ex poiret) and red beet (beta vulgaris l. var. conditiva) cell wall enriched powders. hydrolysis was performed using citric acid at different ph values and reaction times (2 and 3 h). yields obtained for butternut fractions were between 21 and 28 g/100 g; for red beet, yields were 24 and 31 g/100 g for ph ...201020178319
a molecular model for diacylglycerol acyltransferase from mortierella ramanniana var. angulispora.acyl coa diacylglycerol acyltransferase (dgat, ec 2.3.120) is recognized as a key player of cellular diacylglycerol metabolism. it catalyzes the terminal, yet the committed step in triacylglycerol synthesis using diacylglycerol and fatty acyl coa as substrates. the protein sequence of diacylglycerol acyltransferse (dgat) type 2b in moretierella ramanniana var. angulispora (protein_id = aak84180.1) was retrieved from genbank. however, a structure is not yet available for this sequence. the 3d str ...200919759814
structures of new phenolic glycosides from the seeds of cucurbita moschata.a new phenolic glycoside and three known compounds were isolated from the seeds of cucurbita moschata. the structures of the new compound was elucidated as phenylcarbinyl 5-o-(4-hydroxy)benzoyl-beta-d-apiofuranosyl (1-->2)-beta-d-glucopyranoside on the basis of spectroscopic analysis and chemical evidence. three known compounds were identified as 1-o-benzyl[5-o-benzoyl-beta-d-apiofuranosyl(1-->2)]-beta-d-glucopyranoside 2, cucurbitosides c 3 and a 4, by comparison of the spectral data with repor ...200919475995
molecular cloning and expression of a bush related cmv1 gene in tropical pumpkin.a bush-type plant was selected from tropical pumpkin 'cga' (cucurbita moschata duchesne) in order to study the vine development in c. moschata. in this study, a novel gene encoding nadh dehydrogenase was isolated from the vine line (cgav) of c. moschata, that was not expressed in the near isogenic bush line (cgabu). this gene, designated as cmv1 (c. moschata vine 1), was 545 bp in length and was composed of a 477 bp open reading frame, which had 99% nucleotide similarity to the chloroplast ndhj ...201019308673
use of cucumis metuliferus as a rootstock for melon to manage meloidogyne incognita.root-knot nematode-susceptible melons (cantaloupe) were grown in pots with varying levels of meloidogyne incognita and were compared to susceptible melons that were grafted onto cucumis metuliferus or cucurbita moschata rootstocks. in addition, the effect of using melons as transplants in nematode-infested soil was compared to direct seeding of melons in nematode-infested soil. there were no differences in shoot or root weight, or severity of root galling between transplanted and direct-seeded n ...200519262873
tritrophic effects on survival and development in a coccidophagous coccinellid.chilocorus nigritus is currently considered one of the most successful biological control agents of armoured scale insects. however, establishment of this beetle in crop pest situations has not always been successful and there are still gaps in our knowledge of its ecology and behaviour. the research involved an examination of tritrophic effects on the survival and development of this common diaspid predator. the effect of a forced change in host plant on the developmental time of the juvenile s ...200819226777
in vitro effects of cucurbita moschata seed extracts on haemonchus contortus.this study was carried out to evaluate the in vitro effect of cucurbita moschata seed against the parasitic nematode of small ruminants haemonchus contortus. three extracts (aqueous, methanolic and dichloromethane) of c. moschata seed were tested in vitro on four developmental stages of h. contortus using egg hatch assay (eha), larval development assay (lda), l3 migration inhibition (lmi) assay and adult worm motility (awm) assay. the highly significant (p<0.001) ability to stop larval developme ...200919135803
[effects of nacl stress on free polyamines content and reactive oxygen species level in pumpkin roots].taking cucurbita moschata duch hybrid 360-3 x 112-2 and c. ficifolia bouche as test materials, the effects of nacl stress on their plant growth and the o2*- production rate and h2o2 and free polyamines (pas) contents in their roots were studied with hydroponic culture. the results showed that after 10 d nacl stress, the plant growth of the two pumpkin varieties was strongly inhibited, compared with the control, and c. ficifolia was more injured than hybrid 360-3 x 112-2. under nacl stress, the r ...200819102314
location of caspase 3-like protease in the development of sieve element and tracheary element of stem in cucurbita moschata.the casepase is considered to regulate the process of programmed cell death in the development of organisms. in this study, caspase 3-like protease was detected by immunohistochemistry and immunoelectron microscopy during the development of sieve element and tracheary element of stem in cucurbita moschata duch. antibody with brown color (under light microscopy) and gold particles (under transmission electron microscopy) for detecting caspase 3-like protease was mainly displayed in inner phloem, ...200819093968
excellent combination of counter-current chromatography and preparative high-performance liquid chromatography to separate galactolipids from pumpkin.galactolipids in the fruits of cucurbita moschata (pumpkin) could not be completely separated by high-performance liquid chromatography (hplc). preparative hplc was not available for preparing major galactolipid monomers in pumpkin. in the present paper, a combination of high-speed counter-current chromatography (hsccc) and preparative hplc was used for preparing the galactolipids. a fraction containing galactolipids (fr60) from the purification of the n-butanol extract of pumpkin by macro-porou ...200919081570
starch grains on human teeth reveal early broad crop diet in northern peru.previous research indicates that the nanchoc valley in northern peru was an important locus of early and middle holocene human settlement, and that between 9200 and 5500 (14)c yr b.p. the valley inhabitants adopted major crop plants such as squash (cucurbita moschata), peanuts (arachis sp.), and cotton (gossypium barbadense). we report here an examination of starch grains preserved in the calculus of human teeth from these sites that provides direct evidence for the early consumption of cultivat ...200819066222
evaluation of alternatives to methyl bromide in melon crops in guatemala.the monoculture of melon in guatemala has caused the massive appearance of plants with an analogous syndrome for the well-known disease commonly called melon collapse, or vine decline, causing significant losses in crops. methyl bromide is commonly used to sterilize soil prior to planting in guatemala, but it must be phased out by 2015. the objective of this study was to evaluate the technique of grafting melon onto hybrids of cucurbita (cucurbita maxima x cucurbita moschata), as an alternative ...200918998189
ssr-based genetic linkage map of cucurbita moschata and its synteny with cucurbita pepo.the first ssr-based genetic linkage map of cucurbita moschata was created by integrating the maps of two f2 populations with one common parent developed from the crosses waltham butternut (wb) x nigerian local (nl) and zhou (a hull-less type) x wb. the integrated c. moschata map comprises 205 ssr markers and two morphological traits (gr and n). the map is composed of 27 linkage groups with a marker density of 7 cm. comparing the c. moschata map with the published cucurbita pepo map, we found a h ...200818956020
increase in zucchini yellow mosaic virus symptom severity in tolerant zucchini cultivars is related to a point mutation in p3 protein and is associated with a loss of relative fitness on susceptible plants.abstract zucchini yellow mosaic virus (zymv, potyvirus) is a very damaging cucurbit virus worldwide. interspecific crosses with resistant cucurbita moschata have led to the release of "resistant" zucchini squash (c. pepo) f(1) hybrids. however, although the resistance is almost complete in c. moschata, the commercial c. pepo hybrids are only tolerant. zymv evolution toward increased aggressiveness on tolerant hybrids was observed in the field and was obtained experimentally. sequence comparisons ...200318943611
atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of cucurbita moschata.a novel type 1 ribosome-inactivating protein (rip) designated cucurmosin was isolated from the sarcocarp of cucurbita moschata (pumpkin). besides rrna n-glycosidase activity, cucurmosin exhibits strong cytotoxicities to three cancer cell lines of both human and murine origins, but low toxicity to normal cells. plant genomic dna extracted from the tender leaves was amplified by pcr between primers based on the n-terminal sequence and x-ray sequence of the c-terminal. the complete mature protein s ...200818652900
functional group diversity of bee pollinators increases crop yield.niche complementarity is a commonly invoked mechanism underlying the positive relationship between biodiversity and ecosystem functioning, but little empirical evidence exists for complementarity among pollinator species. this study related differences in three functional traits of pollinating bees (flower height preference, daily time of flower visitation and within-flower behaviour) to the seed set of the obligate cross-pollinated pumpkin cucurbita moschata duch. ex poir. across a land-use int ...200818595841
[effects of nacl stress on cation contents in different pumpkin cultivars' seedlings].with the seedlings of 19 pumpkin cultivars as test materials, this paper studied the variations of na+, k+, ca2+, na+/k+, na+/ca2+, sn+, k+ and sna+, ca2+ in their shoots and roots under the stress of 300 mmol nacl x l(-1). the results showed that after an 8-day exposure to 300 mmol nacl x l(-1), the na+ content in the seedlings increased significantly while the k+ content decreased, resulting in the brokenness of ion balance. the root na+ content, shoot na+/k+ and na+/ca2+ ratios, and sna+, k+ ...200818533527
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