Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| higher prevalence of colonization with gardnerella vaginalis and gram-negative anaerobes in patients with recurrent miscarriage and elevated peripheral natural killer cells. | the role of vaginal infections in recurrent miscarriage (rm) is discussed controversially and screening is not recommended in international guidelines. peripheral and uterine nk cells (pnk, unk) play an important role in the establishment of a healthy pregnancy and are targets of immune diagnostics in rm patients. the aim of this study was to analyze the composition of the vaginal microbiota in rm patients and to correlate the findings to clinical characteristics as well as nk cell parameters. i ... | 2017 | 28388469 |
| transient microbiota exposures activate dormant escherichia coli infection in the bladder and drive severe outcomes of recurrent disease. | pathogens often inhabit the body asymptomatically, emerging to cause disease in response to unknown triggers. in the bladder, latent intracellular escherichia coli reservoirs are regarded as likely origins of recurrent urinary tract infection (ruti), a problem affecting millions of women worldwide. however, clinically plausible triggers that activate these reservoirs are unknown. clinical studies suggest that the composition of a woman's vaginal microbiota influences her susceptibility to ruti, ... | 2017 | 28358889 |
| etiology of bacterial vaginosis and polymicrobial biofilm formation. | microorganisms in nature rarely exist in a planktonic form, but in the form of biofilms. biofilms have been identified as the cause of many chronic and persistent infections and have been implicated in the etiology of bacterial vaginosis (bv). bacterial vaginosis is the most common form of vaginal infection in women of reproductive age. similar to other biofilm infections, bv biofilms protect the bv-related bacteria against antibiotics and cause recurrent bv. in this review, an overview of bv-re ... | 2017 | 28358585 |
| gardnerella vaginalis diversity and ecology in relation to vaginal symptoms. | gardnerella vaginalis was first described in 1953, and subsequently identified as the causative agent of a cluster of vaginal symptoms currently known as vaginosis. research has so far failed to confirm whether and by which mechanism g. vaginalis initiates vaginosis, with, consequently, poor diagnostics and treatment outcomes. recent molecular analyses of protein-coding genes demonstrate that the taxon g. vaginalis consists of at least four distinct species. this development may represent a crit ... | 2017 | 28341009 |
| thymbra capitata essential oil as potential therapeutic agent against gardnerella vaginalis biofilm-related infections. | to evaluate the antibacterial activity of thymbra capitata essential oil and its main compound, carvacrol, against gardnerella vaginalis grown planktonically and as biofilms, and its effect of vaginal lactobacilli. | 2017 | 28339292 |
| survival of vaginal microorganisms in three commercially available transport systems. | transport systems are used to collect and maintain the viability of microorganisms. two amies media based transport systems, bd cultureswab™ maxv(+) amies medium without charcoal (maxv(+)) and fisherfinest(®) with amies gel transport medium without charcoal (fisherfinest(®)) were compared to a cary-blair media based transport system, starswab(®) anaerobic transport system (starswab(®)), for their capacity to maintain the viability of 17 clinical microorganisms commonly isolated from the vagina ( ... | 2017 | 28242337 |
| the presence of the putative gardnerella vaginalis sialidase a gene in vaginal specimens is associated with bacterial vaginosis biofilm. | bacterial vaginosis (bv) is a difficult-to-treat recurrent condition in which health-associated lactobacilli are outnumbered by other anaerobic bacteria, such as gardnerella vaginalis. certain genotypes of g. vaginalis can produce sialidase, while others cannot. sialidase is known to facilitate the destruction of the protective mucus layer on the vaginal epithelium by hydrolysis of sialic acid on the glycans of mucous membranes. this process possibly facilitates adhesion of bacterial cells on th ... | 2017 | 28241058 |
| the influence of sexual activity on the vaginal microbiota and gardnerella vaginalis clade diversity in young women. | to examine the influence of sexual activity on the composition and consistency of the vaginal microbiota over time, and distribution of gardnerella vaginalis clades in young women. | 2017 | 28234976 |
| bacterial biofilms in the vagina. | a bacterial biofilm is a structured community of bacteria in a self-produced extracellular matrix, adherent to an inert surface or biological tissue. the involvement of biofilm in a bacterial infection implies that the infection is difficult to treat and that the patient will probably experience relapses of the condition. in bacterial vaginosis (bv), the lactobacilli concentration decreases, while the bacterial load of other (facultative) anaerobic bacteria increases. a hallmark of bv is the pre ... | 2017 | 28232119 |
| determination of antibacterial and technological properties of vaginal lactobacilli for their potential application in dairy products. | functional foods could differently affect human health in relation to the gender. recent studies have highlighted the anti-candida and anti-chlamydia activities of some lactobacillus strains isolated from the vagina of healthy women. considering these important beneficial activities on women's health, the preparation of functional food containing active vaginal lactobacilli can represent a great scientific challenge for the female gender. in this context, the aim of this work was to study some f ... | 2017 | 28223974 |
| gardnerella vaginalis population dynamics in bacterial vaginosis. | bacterial vaginosis (bv) is the leading cause of vaginal discharge and is associated with the facultative gram-variable bacterium gardnerella vaginalis, whose population structure consists of four clades. our goal was to determine if these clades differ with regard to abundance during bv. we performed a short-term longitudinal study of bv. patients were evaluated according to the amsel criteria and nugent scoring at initial diagnosis, immediately after treatment and at a 40- to 45-day follow-up ... | 2017 | 28197729 |
| assessment of atopobium vaginae and gardnerella vaginalis concentrations in a cohort of pregnant south african women. | the purpose of this cross-sectional study was to assess atopobium vaginae and gardnerella vaginalis concentrations in pregnant women of different age groups, gestational age groups, vaginal flora categories and hiv status, and also to determine which dna concentrations best discriminated between bacterial vaginosis (bv)-positive and non-bv categories. | 2017 | 28143901 |
| a molecular portrait of maternal sepsis from byzantine troy. | pregnancy complications are poorly represented in the archeological record, despite their importance in contemporary and ancient societies. while excavating a byzantine cemetery in troy, we discovered calcified abscesses among a woman's remains. scanning electron microscopy of the tissue revealed 'ghost cells', resulting from dystrophic calcification, which preserved ancient maternal, fetal and bacterial dna of a severe infection, likely chorioamnionitis. gardnerella vaginalis and staphylococcus ... | 2017 | 28072390 |
| [nonviral sexually transmitted infections-epidemiology, clinical manifestations, diagnostic workup, therapy : part 3: treponemes, gardnerella and trichomonads]. | in germany, the reported syphilis prevalence has increased continuously since 2010, with a total of 6834 syphilis cases being reported in 2015. the largest increase of reported syphilis occurred in men who have sex with men (msm). the antibiotic agent of choice for treatment of syphilis is still penicillin. there are no penicillin-resistant treponema pallidum strains. alternatives are ceftriaxone and doxycycline. in germany, azithromycin is not approved for treatment of syphilis; however, therap ... | 2017 | 28058468 |
| human three-dimensional endometrial epithelial cell model to study host interactions with vaginal bacteria and neisseria gonorrhoeae. | colonization of the endometrium by pathogenic bacteria ascending from the lower female reproductive tract (frt) is associated with many gynecologic and obstetric health complications. to study these host-microbe interactions in vitro, we developed a human three-dimensional (3-d) endometrial epithelial cell (eec) model using the hec-1a cell line and the rotating wall vessel (rwv) bioreactor technology. our model, composed of 3-d eec aggregates, recapitulates several functional/structural characte ... | 2017 | 28052997 |
| genital injury signatures and microbiome alterations associated with depot medroxyprogesterone acetate usage and intravaginal drying practices. | increasing evidence suggests that depot medroxyprogesterone acetate (dmpa) and intravaginal practices may be associated with an increased risk of hiv infection; however the mechanisms are not fully understood. this study evaluated the effect of dmpa and intravaginal practices on the genital mucosal proteome and microbiome to gain mechanistic insights. | 2016 | 28011908 |
| lactobacillus iners: friend or foe? | the vaginal microbial community is typically characterized by abundant lactobacilli. lactobacillus iners, a fairly recently detected species, is frequently present in the vaginal niche. however, the role of this species in vaginal health is unclear, since it can be detected in normal conditions as well as during vaginal dysbiosis, such as bacterial vaginosis, a condition characterized by an abnormal increase in bacterial diversity and lack of typical lactobacilli. compared to other lactobacillus ... | 2017 | 27914761 |
| subtilosin prevents biofilm formation by inhibiting bacterial quorum sensing. | subtilosin, the cyclic lantibiotic protein produced by bacillus subtilis katmira1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. in this study, subtilosin was purified using ammonium sulfate ((nh4)2so4) precipitation and purified via column chromatography. subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (mic and sub-mic) and anti-biofilm activity (biofilm prevention) were established. subtilosin was evaluated as a quorum ... | 2017 | 27914001 |
| combinations and loads of bacteria affect the cytokine production by fetal membranes: an in vitro study. | the polybacterial invasion and inflammation of the amniotic cavity is a common scenario in ptb, and then, we analyzed the cytokine production by human fetal membranes to better understand the host response to polybacterial infections. | 2016 | 27870156 |
| lysozyme as a cotreatment during antibiotics use against vaginal infections: an in vitro study on gardnerella vaginalis biofilm models. | bacterial vaginoses are frequent in women, most of them involving gardnerella vaginalis. in more than 50% of the cases, usual antibiotic treatments are not capable of eliminating completely the infection, leading to recurrent vaginosis. in addition to the appearance of antibiotic resistance, recurrence can be due to the development of a biofilm by g. vaginalis. in vitro experiments on g. vaginalis biofilms showed that the biofilm protected bacteria from the antibiotic clindamycin. also, recombin ... | 2016 | 27845497 |
| preclinical evaluation of tolerability of a selective, bacteriostatic, locally active vaginal formulation. | polybactum (effik international, brussels, belgium) is a vaginal mucoadhesive product (medical device) designed to form a film that acts as a mechanical barrier with the aim of inhibiting colonization by specific pathogens. it contains polycarbophil, a bioadhesive agent, and lauryl glucoside (lg), a nonionic surfactant that reinforces the barrier effect through its tensioactive properties. | 2016 | 27766122 |
| bacterial vaginosis in association with spontaneous abortion and recurrent pregnancy losses. | bacterial vaginosis (bv) is related to the increased risk of miscarriage, preterm labor, and postpartum endometritis. | 2017 | 27756985 |
| in vitro activity of lactobacillus fermentum lf5 against different candida species and gardnerella vaginalis: a new perspective to approach mixed vaginal infections? | this study was undertaken to demonstrate the ability of lactobacillus fermentum lf5 (dsm 32277) to inhibit in vitro different candida species and gardnerella vaginalis to weigh its potential effectiveness even in mixed vaginal infections. | 2016 | 27741167 |
| evaluation and subsequent optimizations of the quantitative amplisens florocenosis/bacterial vaginosis-frt multiplex real-time pcr assay for diagnosis of bacterial vaginosis. | traditional microscopy-based methods for diagnosis of bacterial vaginosis (bv) are underutilized in many settings, and molecular techniques may provide opportunities for rapid, objective, and accurate bv diagnosis. this study evaluated the quantitative amplisens florocenosis/bacterial vaginosis-frt multiplex real-time pcr (florocenosis-bv) assay. vaginal samples from a previous study including unselected female subjects (n = 163) and using amsel criteria and 454 pyrosequencing for bv diagnosis w ... | 2016 | 27714844 |
| antimicrobial susceptibility and vaginolysin in gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women. | bacterial vaginosis (bv) is a syndrome related to gardnerella vaginalis and is characterized by an imbalance in the vaginal microbiota. this work focused on the evaluation of antimicrobial susceptibility patterns and the occurrence of the vaginolysin (vly) gene in g. vaginalis isolated from bv and non-bv patients. | 2016 | 27694723 |
| genome sequences of 15 gardnerella vaginalis strains isolated from the vaginas of women with and without bacterial vaginosis. | gardnerella vaginalis is a predominant species in bacterial vaginosis, a dysbiosis of the vagina that is associated with adverse health outcomes, including preterm birth. here, we present the draft genome sequences of 15 gardnerella vaginalis strains (now available through bei resources) isolated from women with and without bacterial vaginosis. | 2016 | 27688326 |
| microbiome composition and function drives wound-healing impairment in the female genital tract. | the mechanism(s) by which bacterial communities impact susceptibility to infectious diseases, such as hiv, and maintain female genital tract (fgt) health are poorly understood. evaluation of fgt bacteria has predominantly been limited to studies of species abundance, but not bacterial function. we therefore sought to examine the relationship of bacterial community composition and function with mucosal epithelial barrier health in the context of bacterial vaginosis (bv) using metaproteomic, metag ... | 2016 | 27656899 |
| cervicovaginal cytokines, sialidase activity and bacterial load in reproductive-aged women with intermediate vaginal flora. | studies have shown that not only bacterial vaginosis, but also intermediate vaginal flora has deleterious effects for women's reproductive health. however, literature still lacks information about microbiological and immunological aspects of intermediate flora. | 2016 | 27620141 |
| anti-inflammatory elafin in human fetal membranes. | elafin is a low molecular weight protein with antileukoproteinase, anti-inflammatory, antibacterial and immunomodulating properties. the profile of elafin in fetal membranes is not well characterized. this study determined the changes in elafin expression and concentration in human fetal membrane from patients with preterm prelabor rupture of membranes (pprom) and in vitro in response to intra-amniotic polymicrobial pathogens. | 2017 | 27580179 |
| estimation of gene insertion/deletion rates with missing data. | lateral gene transfer is an important mechanism for evolution among bacteria. here, genome-wide gene insertion and deletion rates are modeled in a maximum-likelihood framework with the additional flexibility of modeling potential missing data. the performance of the models is illustrated using simulations and a data set on gene family phyletic patterns from gardnerella vaginalis that includes an ancient taxon. a novel application involving pseudogenization/genome reduction magnitudes is also ill ... | 2016 | 27565162 |
| gardnerella vaginalis and mollicute detection in rectal swabs from men who have sex with men. | the numbers of rectal sexually transmitted infections are on the rise especially among men who have sex with men. males from men who have sex with men population are encouraged to send a rectal swab to the laboratory for sexually transmitted infection screening at their visit to the genitourinary medicine clinic. in healthy asymptomatic males, the range of pathogens tested is limited therefore other pathogens may be left untreated allowing infections to persist among sexual partners. molecular t ... | 2016 | 27542698 |
| switches in genomic gc content drive shifts of optimal codons under sustained selection on synonymous sites. | the major codon preference model suggests that codons read by trnas in high concentrations are preferentially utilized in highly expressed genes. however, the identity of the optimal codons differs between species although the forces driving such changes are poorly understood. we suggest that these questions can be tackled by placing codon usage studies in a phylogenetic framework and that bacterial genomes with extreme nucleotide composition biases provide informative model systems. switches in ... | 2016 | 27540085 |
| characterisation of probiotic properties in human vaginal lactobacilli strains. | vaginal lactobacilli offer protection against recurrent urinary infections, bacterial vaginosis, and vaginal candidiasis. | 2016 | 27527701 |
| [bacterial vaginosis]. | bacterial vaginosis (bv) is the main cause of vaginal dysbacteriosis in the women during the reproductive age. it is an entity in which many studies have focused for years and which is still open for discussion topics. this is due to the diversity of microorganisms that cause it and therefore, its difficult treatment. bacterial vaginosis is probably the result of vaginal colonization by complex bacterial communities, many of them non-cultivable and with interdependent metabolism where anaerobic ... | 2016 | 27474242 |
| utilization of molecular methods to identify prognostic markers for recurrent bacterial vaginosis. | recurrent bacterial vaginosis (bv) after antimicrobial therapy is a major problem, affecting >50% of patients within 1 year. the objective of this study was to determine if prospective identification of patients at risk for recurrence using molecular methods is feasible. | 2016 | 27431434 |
| analysis of the vaginal microbiome by next-generation sequencing and evaluation of its performance as a clinical diagnostic tool in vaginitis. | next-generation sequencing (ngs) can detect many more microorganisms of a microbiome than traditional methods. this study aimed to analyze the vaginal microbiomes of korean women by using ngs that included bacteria and other microorganisms. the ngs results were compared with the results of other assays, and ngs was evaluated for its feasibility for predicting vaginitis. | 2016 | 27374709 |
| frequency of detection of gardnerella vaginalis in vaginal smears in the upper carniola region. | bacterial vaginosis is of clinical interest because of its possible causal relationship with complications during pregnancy, postpartum, and complications after surgery. | 2016 | 27348455 |
| association of sexual debut in adolescents with microbiota and inflammatory markers. | to investigate the association of sexual debut and vaginal, anorectal, and oral microbiota and vaginal inflammatory markers in female adolescents. | 2016 | 27275789 |
| vaginal dysbiosis from an evolutionary perspective. | evolutionary approaches are powerful tools for understanding human disorders. the composition of vaginal microbiome is important for reproductive success and has not yet been characterized in the contexts of social structure and vaginal pathology in non-human primates (nhps). we investigated vaginal size, vulvovaginal pathology and the presence of the main human subtypes of lactobacillus spp./ bv-related species in the vaginal microflora of baboons (papio spp.). we performed morphometric measure ... | 2016 | 27226349 |
| vaginal microbiota. | the knowledge about the normal and abnormal vaginal microbiome has changed over the last years. culturing techniques are not suitable any more for determination of a normal or abnormal vaginal microbiota. non culture-based modern technologies revealed a complex and dynamic system mainly dominated by lactobacilli.the normal and the abnormal vaginal microbiota are complex ecosystems of more than 200 bacterial species influenced by genes, ethnic background and environmental and behavioral factors. ... | 2016 | 27161352 |
| selection of lactobacillus strains as potential probiotics for vaginitis treatment. | lactobacilli are the dominant bacteria of the vaginal tract of healthy women, and imbalance of the local microbiota can predispose women to acquire infections, such as bacterial vaginosis (bv) and vulvovaginal candidiasis (vvc). although antimicrobial therapy is generally effective, there is still a high incidence of recurrence and increase of microbial resistance due to the repetitive use of antimicrobials. thus, it has been suggested that administration of probiotics incorporating selected lac ... | 2016 | 27154285 |
| screening of compounds against gardnerella vaginalis biofilms. | bacterial vaginosis (bv) is a common infection in reproductive age woman and is characterized by dysbiosis of the healthy vaginal flora which is dominated by lactobacilli, followed by growth of bacteria like gardnerella vaginalis. the ability of g. vaginalis to form biofilms contributes to the high rates of recurrence that are typical for bv and which unfortunately make repeated antibiotic therapy inevitable. here we developed a biofilm model for g. vaginalis and screened a large spectrum of com ... | 2016 | 27111438 |
| molecular assessment of bacterial vaginosis by lactobacillus abundance and species diversity. | to date, women are most often diagnosed with bacterial vaginosis (bv) using microscopy based nugent scoring or amsel criteria. however, the accuracy is less than optimal. the aim of the present study was to confirm the identity of known bv-associated composition profiles and evaluate indicators for bv using three molecular methods. | 2016 | 27107961 |
| the first report: an analysis of bacterial flora of the first voided urine specimens of patients with male urethritis using the 16s ribosomal rna gene-based clone library method. | to analyse the bacterial flora of urine from patients with male urethritis using the clone library method. | 2016 | 27013259 |
| slight pro-inflammatory immunomodulation properties of dendritic cells by gardnerella vaginalis: the "invisible man" of bacterial vaginosis? | bacterial vaginosis (bv), the most common genital infection in reproductive-aged women, is associated with increased risk of sexually transmitted infections. its etiology remains unclear, especially the role of gardnerella (g.) vaginalis, an anaerobic bacterium characteristic of the bv-alteration of the vaginal ecosystem. in the genital mucosa, dendritic cells (dcs) sense bacteria of the microenvironment via receptors and then orchestrate the immune response by induction of different t cell subt ... | 2016 | 26989700 |
| a fruitful alliance: the synergy between atopobium vaginae and gardnerella vaginalis in bacterial vaginosis-associated biofilm. | bacterial vaginosis (bv) is characterised by a change in the microbial composition of the vagina. the bv-associated organisms outnumber the health-associated lactobacillus species and form a polymicrobial biofilm on the vaginal epithelium, possibly explaining the difficulties with antibiotic treatment. a better understanding of vaginal biofilm with emphasis on atopobium vaginae and gardnerella vaginalis may contribute to a better diagnosis and treatment of bv. | 2016 | 26965870 |
| changes in vaginal microbiota and immune mediators in hiv-1-seronegative kenyan women initiating depot medroxyprogesterone acetate. | depot medroxyprogesterone acetate (dmpa) is associated with hiv acquisition. we studied changes in vaginal microbiota and inflammatory milieu after dmpa initiation. | 2016 | 26914908 |
| abnormal vaginal microbiota may be associated with poor reproductive outcomes: a prospective study in ivf patients. | what is the diagnostic performance of qpcr assays compared with nugent scoring for abnormal vaginal microbiota and for predicting the success rate of ivf treatment? | 2016 | 26911864 |
| erratum to: 'screen-and-treat program by point-of-care of atopobium vaginae and gardnerella vaginalis in preventing preterm birth (autop trial): study protocol for a randomized controlled trial'. | 2016 | 26873474 | |
| bacterial vaginosis biofilms: challenges to current therapies and emerging solutions. | bacterial vaginosis (bv) is the most common genital tract infection in women during their reproductive years and it has been associated with serious health complications, such as preterm delivery and acquisition or transmission of several sexually transmitted agents. bv is characterized by a reduction of beneficial lactobacilli and a significant increase in number of anaerobic bacteria, including gardnerella vaginalis, atopobium vaginae, mobiluncus spp., bacteroides spp. and prevotella spp.. bei ... | 2015 | 26834706 |
| development and validation of a highly accurate quantitative real-time pcr assay for diagnosis of bacterial vaginosis. | bacterial vaginosis (bv) is the most common gynecological infection in the united states. diagnosis based on amsel's criteria can be challenging and can be aided by laboratory-based testing. a standard method for diagnosis in research studies is enumeration of bacterial morphotypes of a gram-stained vaginal smear (i.e., nugent scoring). however, this technique is subjective, requires specialized training, and is not widely available. therefore, a highly accurate molecular assay for the diagnosis ... | 2016 | 26818677 |
| mycoplasma hominis and gardnerella vaginalis display a significant synergistic relationship in bacterial vaginosis. | gardnerella vaginalis plays an important role in bacterial vaginosis (bv,) while the role of genital mollicutes is less obvious. the diagnosis of bv by use of the current gram stain nugent score is also suboptimal for defining the role of mollicutes that lack a cell wall. since bacterial load and diversity is an important prerequisite for bv, real-time quantitative polymerase chain reaction (qpcr) assays enable these to be assessed. the purpose of this study was to define the role of genital mol ... | 2016 | 26796553 |
| the common vaginal commensal bacterium ureaplasma parvum is associated with chorioamnionitis in extreme preterm labor. | to assess the association of vaginal commensal and low-grade pathogenic bacteria including ureaplasma parvum, ureaplasma urealyticum, mycoplasma hominis, mycoplasma genitalium, group b streptococcus (gbs), and gardnerella vaginalis, in women who delivered preterm at less than 37-week gestation in the presence or absence of inflammation of the chorioamnionitic membranes. | 2016 | 26795404 |
| affirm vpiii microbial identification test can be used to detect gardnerella vaginalis, candida albicans and trichomonas vaginalis microbial infections in korean women. | the aim of this study was to compare affirm vpiii microbial identification test results for korean women to those obtained for gardnerella vaginalis through nugent score, candida albicans based on vaginal culture and trichomonas vaginalis based on wet smear diagnostic standards. | 2016 | 26787446 |
| escherichia coli and enterococcus faecalis are able to incorporate and enhance a pre-formed gardnerella vaginalis biofilm. | gardnerella vaginalis is the most frequent microorganism found in bacterial vaginosis (bv), while escherichia coli and enterococcus faecalis are amongst the most frequent pathogens found in urinary tract infections (utis). this study aimed to evaluate possible interactions between utis pathogens and g. vaginalis using an in vitro dual-species biofilm model. our results showed that dual-species biofilms reached significantly higher bacterial concentration than monospecies biofilms. moreover, visu ... | 2016 | 26782142 |
| gardnerella vaginalis subgroups defined by cpn60 sequencing and sialidase activity in isolates from canada, belgium and kenya. | increased abundance of gardnerella vaginalis and sialidase activity in vaginal fluid is associated with bacterial vaginosis (bv), a common but poorly understood clinical entity associated with poor reproductive health outcomes. since most women are colonized with g. vaginalis, its status as a normal member of the vaginal microbiota or pathogen causing bv remains controversial, and numerous classification schemes have been described. since 2005, sequencing of the chaperonin-60 universal target (c ... | 2016 | 26751374 |
| prevalence of 7 sexually transmitted organisms by multiplex real-time pcr in fallopian tube specimens collected from saudi women with and without ectopic pregnancy. | ectopic pregnancy (ep) is associated with maternal morbidity and occasionally mortality during the first trimester. a history of sexually transmitted infection (sti) and pelvic inflammatory disease have been implicated as major risk factors for ep. our aim was to measure the prevalence of chlamydia trachomatis (ct), neisseria gonorrhoeae, mycoplasma genitalium (mg), ureaplasma parvum/urealyticum, gardnerella vaginalis, trichomonas vaginalis and herpes simplex virus (hsv)-1&2 in fallopian tubes c ... | 2015 | 26666587 |
| lactobacillus crispatus inhibits growth of gardnerella vaginalis and neisseria gonorrhoeae on a porcine vaginal mucosa model. | the vaginal microbiota can impact the susceptibility of women to bacterial vaginosis (bv) and sexually transmitted infections (stis). bv is characterized by depletion of lactobacillus spp., an overgrowth of anaerobes (often dominated by gardnerella vaginalis) and a ph > 4.5. bv is associated with an increased risk of acquiring stis such as chlamydia and gonorrhea. while these associations have been identified, the molecular mechanism(s) driving the risk of infections are unknown. an ex vivo porc ... | 2015 | 26652855 |
| a dna tool for early detection of vaginal dysbiosis in african women. | a next-generation diagnostic tool for bacterial vaginosis, consisting of quantitative and/or qualitative molecular criteria, has not yet been identified. the optimal diagnostic tool should not only diagnose bacterial vaginosis in diverse populations, but should also detect early signs of transition to dysbiosis. we evaluated a tool based on log10-transformed qpcr data for lactobacillus crispatus, lactobacillus iners, lactobacillus jensenii, lactobacillus gasseri, lactobacillus vaginalis, lactoba ... | 2016 | 26577657 |
| draft genome sequence of a metronidazole-resistant derivative of gardnerella vaginalis strain atcc 14019. | we report the genome sequence of a metronidazole-resistant derivative of gardnerella vaginalis atcc 14019. this strain was obtained after serial selection to increase the mic from 4 to ≥500 µg/ml. two coding changes, in genes encoding a response regulator and an nad(+) synthetase, arose during selection. | 2015 | 26564054 |
| identification of intrinsically metronidazole-resistant clades of gardnerella vaginalis. | gardnerella vaginalis is associated with bacterial vaginosis (bv), the most common cause of vaginal discharge. metronidazole is a front-line therapy for bv, and treatment failure and recurrent disease are common problems. whole-genome sequencing studies have revealed that g. vaginalis has a population structure that consists of 4 clades: clades 1 and 3 are associated with bv, whereas clades 2 and 4 are not. to determine if metronidazole susceptibility is associated with population structure, we ... | 2016 | 26514076 |
| bv and non-bv associated gardnerella vaginalis establish similar synergistic interactions with other bv-associated microorganisms in dual-species biofilms. | dual-species biofilm formation between gardnerella vaginalis strains isolated from women with or without bacterial vaginosis (bv) and other 24 bv-associated microorganisms support that the key difference in virulence potential between bv-negative and bv-positive g. vaginalis strains seems not to be related with biofilm maturation. | 2015 | 26505928 |
| bovine vaginal strain kocuria kristinae and its characterization. | kocuria spp. are widely distributed in nature. they are gram-positive, coagulase-negative, coccoid bacteria belonging to the family micrococcaceae, suborder micrococcineae, order actinomycetales, class actinobacteria. in general, limited knowledge exists concerning the properties associated with the representants of the genus kocuria, kocuria kristinae as well. following our previous results, k. kristinae kk2014 biocenol(™) (ccm 8628) was isolated from vagina of a healthy cow. its taxonomical al ... | 2016 | 26494240 |
| screen-and-treat program by point-of-care of atopobium vaginae and gardnerella vaginalis in preventing preterm birth (autop trial): study protocol for a randomized controlled trial. | international recommendations in favor of screening for vaginal infection in pregnancy are based on heterogeneous criteria. in most developed countries, the diagnosis of bacterial vaginosis is only recommended for women with high-risk of preterm birth. the nugent score is currently used, but molecular quantification tools have recently been reported with a high sensitivity and specificity. their value for reducing preterm birth rates and related complications remains unexplored. this trial was d ... | 2015 | 26482128 |
| gardnerella vaginalis: an overlooked pathogen in male patients? | 2015 | 26472061 | |
| enhanced trapping of hiv-1 by human cervicovaginal mucus is associated with lactobacillus crispatus-dominant microbiota. | cervicovaginal mucus (cvm) can provide a barrier that precludes hiv and other sexually transmitted virions from reaching target cells in the vaginal epithelium, thereby preventing or reducing infections. however, the barrier properties of cvm differ from woman to woman, and the causes of these variations are not yet well understood. using high-resolution particle tracking of fluorescent hiv-1 pseudoviruses, we found that neither ph nor nugent scores nor total lactic acid levels correlated signif ... | 2015 | 26443453 |
| comparison of cervico-vaginal colonization among sexually active women by intrauterine device use. | in this study we aimed to evaluate the effect of intrauterine devices (iuds) on cervico-vaginal colonization. | 2015 | 26409733 |
| co-infection of human immunodeficiency virus and sexually transmitted infections in circumcised and uncircumcised cases in india. | acquired immunodeficiency syndrome (aids), is now one of the greatest challenges facing the world. sexual transmission is the primary route of human immunodeficiency virus infection worldwide. male circumcision is being considered as strategy to reduce the burden of hiv/aids. | 2014 | 26396445 |
| evaluation of risk factors in patients attending sti clinic in a tertiary care hospital in north india. | in the past few years, the interest in stds and their management has increased tremendously because of their proven role in facilitation of hiv infection, which, in turn, also increases the risk of acquiring stis. sexually transmitted diseases (stds) are a major health problem affecting mostly young people, not only in developing, but also in developed countries male circumcision is being considered as strategy to reduce the burden of hiv/aids. | 2015 | 26392654 |
| a multi-platform metabolomics approach identifies highly specific biomarkers of bacterial diversity in the vagina of pregnant and non-pregnant women. | bacterial vaginosis (bv) increases transmission of hiv, enhances the risk of preterm labour, and is associated with malodour. clinical diagnosis often relies on microscopy, which may not reflect the microbiota composition accurately. we use an untargeted metabolomics approach, whereby we normalize the weight of samples prior to analysis, to obtained precise measurements of metabolites in vaginal fluid. we identify biomarkers for bv with high sensitivity and specificity (auc = 0.99) in a cohort o ... | 2015 | 26387596 |
| optimization of culture conditions for gardnerella vaginalis biofilm formation. | bacterial vaginosis is the leading vaginal disorder in women in reproductive age. although bacterial vaginosis is related with presence of a biofilm composed predominantly by gardnerella vaginalis, there has not been a detailed information addressing the environmental conditions that influence the biofilm formation of this bacterial species. here, we evaluated the influence of some common culture conditions on g. vaginalis biofilm formation, namely inoculum concentration, incubation period, feed ... | 2015 | 26381661 |
| [bacteremia by gardnerella vaginalis in a case of complicated urolithiasis]. | 2017 | 26364858 | |
| draft genome sequence of a metronidazole-resistant gardnerella vaginalis isolate. | we report the draft genome sequence of a gardnerella vaginalis strain (3549624) isolated from a vaginal specimen. g. vaginalis is associated with bacterial vaginosis, the most common cause of vaginal discharge, which is often treated with metronidazole. this isolate is highly resistant to metronidazole (mic, 500 µg/ml) and may be useful for comparative genomic studies to determine the molecular basis of metronidazole resistance in this species. | 2015 | 26337887 |
| unravelling the bacterial vaginosis-associated biofilm: a multiplex gardnerella vaginalis and atopobium vaginae fluorescence in situ hybridization assay using peptide nucleic acid probes. | bacterial vaginosis (bv), a condition defined by increased vaginal discharge without significant inflammation, is characterized by a change in the bacterial composition of the vagina. lactobacillus spp., associated with a healthy vaginal microbiome, are outnumbered by bv-associated organisms. these bacteria could form a polymicrobial biofilm which allows them to persist in spite of antibiotic treatment. in this study, we examined the presence of gardnerella vaginalis and atopobium vaginae in vag ... | 2015 | 26305575 |
| new assay for gardnerella vaginalis loads correlates with nugent scores and has potential in the diagnosis of bacterial vaginosis. | gardnerella vaginalis is a gram-variable anaerobic bacterium present in 100% of women with bacterial vaginosis (bv). bv is a complex polymicrobial condition with no single causative agent. the current laboratory detection method for bv relies on a gram-stain nugent score to estimate the quantity of different bacterial morphotypes in the vaginal micro flora. whilst the nugent score can distinguish between women with and without bv, a significant proportion are categorized as intermediate, which f ... | 2015 | 26296660 |
| changes in vaginal microbiota following antimicrobial and probiotic therapy. | the composition of the vaginal microbiota is known to be important for health. when infections occur, antimicrobial therapy is often poorly efficacious. | 2015 | 26282697 |
| sexually transmitted pathogens, depression, and other manifestations associated with premenstrual syndrome. | this study investigated whether sexually transmitted infections and lifestyle variables are associated with premenstrual syndrome (pms) as well as particular manifestations commonly associated with pms. data were gathered from medical records of 500 regularly cycling women. the following infectious agents were investigated: human papillomavirus, chlamydia trachomatis, neisseria gonorrheae, gardnerella vaginalis, candida albicans, and trichomonas vaginalis. bivariate tests and multivariate logist ... | 2015 | 26272230 |
| effects of a one year reusable contraceptive vaginal ring on vaginal microflora and the risk of vaginal infection: an open-label prospective evaluation. | a contraceptive vaginal ring (cvr) containing nestorone® (nes) and ethinyl estradiol (ee) that is reusable for 1- year (13 cycles) is under development. this study assessed effects of this investigational cvr on the incidence of vaginal infections and change in vaginal microflora. | 2015 | 26267119 |
| a multiplex real-time pcr assay for routine diagnosis of bacterial vaginosis. | a semi-quantitative multiplex pcr assay for the diagnosis of bacterial vaginosis (bv) was evaluated in a prospective study in a population of dutch women with complaints of abnormal vaginal discharge. the pcr targets gardnerella vaginalis, atopobium vaginae, megasphaera phylotype 1, lactobacillus crispatus and lactobacillus iners. together with a short questionnaire, a vaginal swab for pcr and vaginal smear for microscopy were taken by their general practitioner or gynaecologist. data from 151 w ... | 2015 | 26143346 |
| combination of microbiological culture and multiplex pcr increases the range of vaginal microorganisms identified in cervical cancer patients at high risk for bacterial vaginosis and vaginitis. | bacterial vaginosis (bv) and vaginitis in cervical cancer patients might becaused by mixed aerobic, anaerobic, and atypical bacteria. since genital tract infections can be complicated, early and accurate identification of causal pathogens is vital. | 2015 | 26117968 |
| using an in-vitro biofilm model to assess the virulence potential of bacterial vaginosis or non-bacterial vaginosis gardnerella vaginalis isolates. | gardnerella vaginalis is the most common species found in bacterial vaginosis (bv). however, it is also present in a significant proportion of healthy women and g. vaginalis vaginal colonization does not always lead to bv. in an effort to better understand the differences between g. vaginalis isolated from women with a positive (bv) versus a negative (non-bv) diagnosis of bv, we compared the virulence potential of 7 bv and 7 non-bv g. vaginalis isolates and assessed the virulence factors related ... | 2015 | 26113465 |
| influence of biofilm formation by gardnerella vaginalis and other anaerobes on bacterial vaginosis. | bacterial vaginosis (bv) is the worldwide leading vaginal disorder among women of reproductive age. bv is characterized by the replacement of beneficial lactobacilli and the augmentation of anaerobic bacteria. gardnerella vaginalis is a predominant bacterial species, but bv is also associated with other numerous anaerobes, such as atopobium vaginae, mobiluncus mulieris, prevotella bivia, fusobacterium nucleatum, and peptoniphilus species. currently, the role of g. vaginalis in the etiology of bv ... | 2015 | 26080369 |
| sensitive detection of thirteen bacterial vaginosis-associated agents using multiplex polymerase chain reaction. | bacterial vaginosis (bv) is characterized by a polymicrobial proliferation of anaerobic bacteria and depletion of lactobacilli, which are components of natural vaginal microbiota. currently, there are limited conventional methods for bv diagnosis, and these methods are time-consuming, expensive, and rarely allow for the detection of more than one agent simultaneously. therefore, we conceived and validated a multiplex polymerase chain reaction (m-pcr) assay for the simultaneous screening of thirt ... | 2015 | 26078959 |
| the sactibiotic subclass of bacteriocins: an update. | the sactibiotics are a recently designated subclass of bacteriocins that contain characteristic cysteine sulphur to α -carbon linkages mediated through post-translational modifications. they are a relatively small subclass of bacteriocins compared to the most thoroughly studied lantibiotics. the sactibiotics that have been extensively studied thus far are thuricin cd, subtilosin a, thurincin h, and propionicin f. despite their recent discovery, there have already been significant advances made i ... | 2015 | 26031307 |
| prevalence of the genital tract bacterial infections after vaginal reconstructive surgery. | due to frequent childbirth, heavy lifting and the structure of the lives of rural women in shahrekord region, iran, cystocele and rectocele are of the main medical problems of the women in this area and for its correction, vaginal reconstructive surgery is needed which causes infection. the purpose of this study was to identify the bacteria causing infection after vaginal reconstructive surgery and performing antibiogram to help these patients for faster recovery. patients enrolled this study we ... | 2014 | 26031026 |
| prevalence of sexually transmitted pathogens associated with hpv infection in cervical samples in a mexican population. | cervical cancer development has been mainly associated with persistent human papillomavirus (hpv) infections. however, hpv infection is unlikely to be sufficient to cause cervical cancer, and the contribution of other sexually transmitted infections (stis) could be the determining factor for cervical lesion-progression. the aim of this study was to estimate the prevalence of stis associated with hpv-positivity in 201 cervical samples from patients who underwent annual routine gynecological exams ... | 2015 | 26010580 |
| cervical microbiota in women with preterm prelabor rupture of membranes. | to analyze the cervical microbiota in women with preterm prelabor rupture of membranes (pprom) by pyrosequencing and to document associations between cervical microbiota, cervical inflammatory response, microbial invasion of the amniotic cavity (miac), histological chorioamnionitis, and intraamniotic infection (iai). | 2015 | 25993616 |
| role of gardnerella vaginalis as an etiological agent of bacterial vaginosis. | bacterial vaginosis is a risk factor for obstetric infections, various adverse outcomes of pregnancy and pelvic inflammatory disease. the objectives of this study were to assess the prevalence of bacterial vaginosis in women attending gynaecology outpatient department (o.p.d) and sexually transmitted disease (s.t.d.) clinic and to assess the role of gardnerella vaginalis as an etiological agent of bacterial vaginosis. | 2014 | 25926959 |
| severe pelvic abscess formation following caesarean section. | we report a case of a 24-year-old woman with severe pelvic abscess formation 2 weeks after secondary caesarean section. the isolated pathogens were a mixture of gardnerella vaginalis, mycoplasma hominis and ureaplasma urealyticum. after initial resistance to systemic antibiotic treatment, she underwent radiologically-guided drainage of the abscesses, whereon she had a continuous recovery. | 2015 | 25911355 |
| diagnosing inflammation and infection in the urinary system via proteomics. | current methodology for the diagnosis of diseases in the urinary system includes patient symptomology, urine analysis and urine culture. asymptomatic bacteriuria from urethral colonization or indwelling catheters, sample contamination from perineal or vaginal sources, and non-infectious inflammatory conditions can mimic utis, leading to uncertainty on medical treatment decisions. | 2015 | 25889401 |
| prevalence of mycoplasma genitalium and mycoplasma hominis in urogenital tract of brazilian women. | the role of mycoplasma hominis and m. genitalium in urogenital tract infections remains unknown. furthermore these mollicutes present a complex relationship with the host immune response. the role of inflammatory cytokines in infections also makes them good candidates to investigate bacterial vaginosis and mycoplasma genital infections. therefore, the aim of this study was to detect the above-mentioned mollicutes by quantitative polymerase chain reaction (qpcr) methodologies in vaginal swabs and ... | 2015 | 25886914 |
| the significance of lactobacillus crispatus and l. vaginalis for vaginal health and the negative effect of recent sex: a cross-sectional descriptive study across groups of african women. | women in sub-saharan africa are vulnerable to acquiring hiv infection and reproductive tract infections. bacterial vaginosis (bv), a disruption of the vaginal microbiota, has been shown to be strongly associated with hiv infection. risk factors related to potentially protective or harmful microbiota species are not known. | 2015 | 25879811 |
| complementary seminovaginal microbiome in couples. | the genital tract microbiome is tightly associated with reproductive health. although many research studies have been performed on the vaginal microbiome, current knowledge of the male microbiome is scarce, and parallel studies examining couples are extremely rare. in this work, we aimed to compare seminal and vaginal microbiomes in couples and to assess the influence of sexual intercourse on vaginal microbiome. the study included 23 couples. microbiomes of semen and vaginal fluid (pre- and post ... | 2015 | 25869222 |
| complete genome sequence of gardnerella vaginalis strain jcm 11026t, isolated from vaginal tracts of women. | gardnerella vaginalis strain jcm 11026(t) was isolated from vaginal tracts of women. here, we report the complete genome sequence of this organism. | 2015 | 25858849 |
| natural antimicrobials subtilosin and lauramide arginine ethyl ester synergize with conventional antibiotics clindamycin and metronidazole against biofilms of gardnerella vaginalis but not against biofilms of healthy vaginal lactobacilli. | the purpose of this study was to evaluate the ability of clindamycin and metronidazole to synergize with natural antimicrobials against biofilms of bacterial vaginosis (bv)-associated gardnerella vaginalis. minimum bactericidal concentrations for biofilm cells (mbcs-b) were determined for each antimicrobial. the mbcs-b of lauramide arginine ethyl ester (lae), subtilosin, clindamycin and metronidazole were 50, 69.5, 20 and 500 μg ml(-1), respectively. a checkerboard assay and isobologram were use ... | 2015 | 25838136 |
| vaginal microbiota of adolescent girls prior to the onset of menarche resemble those of reproductive-age women. | puberty is an important developmental stage wherein hormonal shifts mediate the physical and physiological changes that lead to menarche, but until now, the bacterial composition of vaginal microbiota during this period has been poorly characterized. we performed a prospective longitudinal study of perimenarcheal girls to gain insight into the timing and sequence of changes that occur in the vaginal and vulvar microbiota during puberty. the study enrolled 31 healthy, premenarcheal girls between ... | 2015 | 25805726 |
| [semen quality and its influencing factors in preconception males in nanjing area]. | to investigate the semen quality and its influencing factors in preconception males in nanjing area so as to provide some evidence for working out effective intervention measures. | 2015 | 25796688 |
| the association of uterine cervical microbiota with an increased risk for cervical intraepithelial neoplasia in korea. | recent studies have suggested potential roles of the microbiome in cervicovaginal diseases. however, there has been no report on the cervical microbiome in cervical intraepithelial neoplasia (cin). we aimed to identify the cervical microbiota of korean women and assess the association between the cervical microbiota and cin, and to determine the combined effect of the microbiota and human papillomavirus (hpv) on the risk of cin. the cervical microbiota of 70 women with cin and 50 control women w ... | 2015 | 25752224 |
| diagnosis of bacterial vaginosis by a new multiplex peptide nucleic acid fluorescence in situ hybridization method. | bacterial vaginosis (bv) is one of most common vaginal infections. however, its diagnosis by classical methods reveals low specificity. our goal was to evaluate the accuracy diagnosis of 150 vaginal samples with research gold standard methods and our peptide nucleic acid (pna) probes by fluorescence in situ hybridization (fish) methodology. also, we described the first pna-fish methodology for bv diagnosis, which provides results in approximately 3 h. the results showed a sensitivity of 84.6% (9 ... | 2015 | 25737820 |
| clinical chorioamnionitis at term i: microbiology of the amniotic cavity using cultivation and molecular techniques. | the objectives of this study were: 1) to determine the amniotic fluid (af) microbiology of patients with the diagnosis of clinical chorioamnionitis at term using both cultivation and molecular techniques; and 2) to examine the relationship between intra-amniotic inflammation with and without microorganisms and placental lesions consistent with acute af infection. | 2015 | 25720095 |