Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| genotypic analysis of tropheryma whipplei from patients with whipple disease in the americas. | tropheryma whipplei, the agent of whipple disease, causes a rare bacterial disease that may be fatal if not treated. the classical form of the disease includes diarrhoea, weight loss, arthritis, endocarditis and neurological manifestations. genotyping studies done in europe, africa and asia showed high genetic diversity with no correlation between genotypes and clinical features, but contributed to a better understanding of the epidemiology of the disease. more than 70 genotypes have been descri ... | 2017 | 28385924 |
| laboratory approach to the diagnosis of culture-negative infective endocarditis. | blood-culture negative endocarditis (bcne) accounts for up to 35% of all cases of infective endocarditis (ie) and is a serious life-threatening condition with considerable morbidity and mortality. rapid detection and identification of the causative pathogen is essential for timely, directed therapy. blood-culture negative endocarditis presents a diagnostic and therapeutic challenge. causes of bcne are varied including: treatment with antibiotic agents prior to blood culture collection; sub-optim ... | 2017 | 28372886 |
| clinical manifestations, treatment, and diagnosis of tropheryma whipplei infections. | whipple's disease is a rare infectious disease that can be fatal if left untreated. the disease is caused by infection with tropheryma whipplei, a bacterium that may be more common than was initially assumed. most patients present with nonspecific symptoms, and as routine cultivation of the bacterium is not feasible, it is difficult to diagnose this infection. on the other hand, due to the generic symptoms, infection with this bacterium is actually quite often in the differential diagnosis. the ... | 2017 | 28298472 |
| bilateral ocular myositis associated with whipple's disease. | to describe the clinical features of a caucasian female patient with a history of treated gastrointestinal whipple's disease (wd) who developed new-onset diplopia, with a description of the histopathological features of the extraocular muscle biopsies. | 2017 | 28275598 |
| acute infections caused by tropheryma whipplei. | tropheryma whipplei is the causative bacterium of whipple's disease. its first culture has led to an enlargement of the field of the caused infections. here, we comprehensively review acute t. whipplei infections. in a cohort study featuring 4000 children, t. whipplei was significantly more common in patients with diarrhea (4%) than in those without (1.7%). a case-controlled study highlighted 58 patients suffering from pneumonia with the detection of t. whipplei in their bronchoalveolar fluids. ... | 2017 | 28262041 |
| what is the best therapy for whipple's disease? our point of view. | although whipple's disease (wd) has been treated with antibiotics since the early 50s, the best antibiotics and the duration of the therapy have not yet been established. we consider here the pro and cons of the two most commonly used therapies, ceftriaxone followed by trimethoprim-sulfamethoxazole (tmp-smz) and hydroxychloroquine in combination with doxycycline. the therapy based on ceftriaxone and tmp-smz is efficient in the vast majority of patients for the first few years. however, since rei ... | 2017 | 27924649 |
| deep vein thrombosis as initial manifestation of whipple disease. | wipple disease (wd) is a rare chronic disease caused by the bacillus tropheryma whipplei. constitutive, rheumatologic, gastrointestinal, cardiac, cerebral, lymphatic, cutaneous, and ophthalmological signs are possible systemic symptoms. however, thrombotic manifestations are rarely described as "stroke-like syndrome" or arterial thrombosis. diagnosis is based on clinical manifestations and pathological examination. laboratory findings may include anemia, leukocytosis, and thrombocytosis. | 2017 | 27920655 |
| whipple's disease. | in recent years, it has become apparent that tropheryma whipplei not only causes a chronic multisystemic infection which is often preceded by arthropathies for many years, well known as 'classical' whipple's disease, but also clinically becomes manifest with localized organ affections and acute (transient) infections in children. t. whipplei is found ubiquitously in the environment and colonizes in some healthy carriers. in this review, we highlight new aspects of this enigmatic infectious disor ... | 2016 | 27884091 |
| whipple's disease diagnosed using electron microscopy and polymerase chain reaction. | a 50-year-old man presented with bloody diarrhea and 25-kg weight loss over 3 months. upper and lower endoscopy showed diffuse shaggy white villi in the duodenum and terminal ileum. in addition, capsule endoscopy and double-balloon enteroscopy revealed shaggy white villi in the entire small intestine. histological examination of biopsy specimens found the lamina propria of the duodenal and intestinal mucosa to be densely infiltrated by rich foamy macrophages that were periodic acid-schiff-positi ... | 2017 | 27829601 |
| the bronchial epithelial cell bacterial microbiome and host response in patients infected with human immunodeficiency virus. | chronic obstructive pulmonary disease (copd) is an important comorbidity in patients living with human immunodeficiency virus (hiv). previous bacterial microbiome studies have shown increased abundance of specific bacterium, like tropheryma whipplei, and no overall community differences. however, the host response to the lung microbiome is unknown in patients infected with hiv. | 2016 | 27829448 |
| 2016 | 27705184 | ||
| culture negative infective endocarditits: a changing paradigm. | traditionally, the modified duke's criteria, based primarily on positive blood cultures, is used to diagnose infective endocarditis (ie). however, reports demonstrate that 31% of cases are diagnosed as culture negative infective endocarditis (cnie)1. consequently, empiric broad-spectrum antibiotics are prescribed to cover unidentified organisms and, as a result, antibiotic therapy may be compromised. molecular diagnostic techniques aid with identifying causative organisms in cases of cnie and we ... | 2016 | 27685880 |
| whipple's arthritis. | whipple's disease is a chronic systemic infection that is due to the bacterial agent tropheryma whipplei and can be cured by appropriate antibiotic therapy. the typical patient is a middle-aged man. rheumatologists are in a prime position to handle whipple's disease. the classical presentation combines weight loss and diarrhea, preceded in three-quarters of patients by a distinctive pattern of joint manifestations that run an intermittent course, at least initially. the mean time from joint symp ... | 2016 | 27502365 |
| tropheryma whipplei dna in bronchoalveolar lavage samples: a case control study. | tropheryma whipplei, the causative bacterium of whipple's disease, can cause acute pneumonia. we performed a case-control study including patients with t. whipplei in bronchoalveolar lavages (bals) and controls in order to compare patients' clinical statuses. we tested t. whipplei pcr from january 2013 to december 2014, in all the 1438 bals in marseille, france. controls were hospitalized in the same unit during the same period and were comparable in age and sex. eighty-eight bals (6.1%) were po ... | 2016 | 27432769 |
| presence of tropheryma whipplei in different body sites in a cohort of healthy subjects. | 2016 | 27420361 | |
| tropheryma whipplei associated with diarrhoea in young children. | tropheryma whipplei was detected in preliminary studies in faeces of young children with diarrhoea and also in faeces of asymptomatic persons, not only in europe but also in africa. in this study, the link between this bacterium and the presence of acute diarrhoea was evaluated in a large group of children. from december 2009 to january 2013, rectal swabs collected from 3796 children in the emergency departments of university hospitals in marseille, france, were analysed: 555 children (245 femal ... | 2016 | 27404363 |
| pcr analysis is superior to histology for diagnosis of whipple's disease mimicking seronegative rheumatic diseases. | the diagnosis of whipple's disease (wd) is commonly confirmed by histology demonstrating periodic acid schiff (pas)-positive macrophages in the duodenal mucosa. analysis of intestinal tissue or other specimens using polymerase chain reaction (pcr) is a more sensitive method. however, the relevance of positive pcr findings is still controversial. therefore, we evaluated the relevance of histology and pcr findings to establishing the diagnosis of wd in a series of wd patients initially presenting ... | 2017 | 27398638 |
| longitudinal analysis of the lung microbiota of cynomolgous macaques during long-term shiv infection. | longitudinal studies of the lung microbiome are challenging due to the invasive nature of sample collection. in addition, studies of the lung microbiome in human disease are usually performed after disease onset, limiting the ability to determine early events in the lung. we used a non-human primate model to assess lung microbiome alterations over time in response to an hiv-like immunosuppression and determined impact of the lung microbiome on development of obstructive lung disease. cynomolgous ... | 2016 | 27391224 |
| tropheryma whipplei endocarditis in spain: case reports of 17 prospective cases. | tropheryma whipplei endocarditis is an uncommon condition with very few series and <90 cases reported in the literature. the aim of the study was to analyze the epidemiological, clinical, and outcome characteristics of 17 cases of t. whipplei endocarditis recruited in our country from a multicentric cohort from 25 spanish hospitals from the spanish collaboration on endocarditis-grupo de apoyo al manejo de la endocarditis infecciosa en españa.from a total of 3165 cases included in the cohort, 14. ... | 2016 | 27368042 |
| tropheryma whipplei as a cause of epidemic fever, senegal, 2010-2012. | the bacterium tropheryma whipplei, which causes whipple disease in humans, is commonly detected in the feces of persons in africa. it is also associated with acute infections. we investigated the role of t. whipplei in febrile patients from 2 rural villages in senegal. during june 2010-march 2012, we collected whole-blood finger-prick samples from 786 febrile and 385 healthy villagers. t. whipplei was detected in blood specimens from 36 (4.6%) of the 786 febrile patients and in 1 (0.25%) of the ... | 2016 | 27314980 |
| [whipple's disease: a case of postmortem diagnosis]. | the paper describes a case of the postmortem diagnosis of whipple's disease, a rare bacterial infection, in a patient with multiple organ dysfunction showing intestinal manifestations with massive retroperitoneal lymphadenopathy. histochemical and electron microscopic methods were used to examine the patient, which showed the morphological changes occurring in the lymph nodes and bowel and the ultrastructural characteristics of tropheryma whipplei while examining the sections primarily fixed in ... | 2017 | 27296007 |
| tropheryma whipplei infection (whipple's disease) in a patient after liver transplantation. | whipple's disease (wd) is a rare infection caused by the bacterium tropheryma whipplei that can affect multiple organs and most commonly occurs in the immunocompetent host. only 3 cases of wd have been reported in the setting of immunosuppression for organ transplantation. here, we report the first case of wd, to our knowledge, in a patient after liver transplantation with comorbid graft-versus-host-disease. we discuss the diagnostic challenges in this setting and the value of electron microscop ... | 2016 | 27258480 |
| [whipple disease: a differential diagnosis of polyarthritis to keep in mind]. | whipple disease is a rare and potentially fatal bacterial infection induced by a gram-positive bacillus, tropheryma whipplei. it is responsible for articular, digestive, neurological, ophthalmological and cardiological symptoms that occur either concomitant or isolated. thus, the diagnosis is difficult to make and therefore often delayed. a paradoxal exacerbation of the symptoms under immunosuppressive drugs such as glucocorticoids and anti-tnf mab, or inexpected improvements under antibiotic tr ... | 2016 | 27197326 |
| co-circulation of plasmodium and bacterial dnas in blood of febrile and afebrile children from urban and rural areas in gabon. | malaria is considered to be the most common etiology of fever in sub-saharan africa while bacteremias exist but are under assessed. this study aimed to assess bacteremias and malaria in children from urban and rural areas in gabon. dna extracts from blood samples of 410 febrile and 60 afebrile children were analyzed using quantitative polymerase chain reaction. plasmodium spp. was the microorganism most frequently detected in febrile (78.8%, 323/410) and afebrile (13.3%, 8/60) children, (p < 0.0 ... | 2016 | 27114297 |
| deglycosylation of tropheryma whipplei biofilm and discrepancies between diagnostic results during whipple's disease progression. | whipple's disease is a systemic infectious disease associated with the bacterium tropheryma whipplei. numerous reports have presented puzzling discrepancies between diagnosis methods. we addressed this confusion using fluorescent in situ hybridization and immunofluorescence assays to evaluate 34 duodenal biopsies and 1 lymph node biopsy from whipple's patients. we showed the presence of bacteria in both ck20(+) epithelial cells and cd68(+) macrophages. bacteria are found embedded in a biofilm hi ... | 2016 | 27025850 |
| rampant infections of bone marrow stem cell niches as triggers for spondyloarthropathies and rheumatoid arthritis. | tropheryma whipplei can induce rheumatism mimicking spa or ra, but even more rampant bacterial/viral infections in epiphyseal bones could also contribute to the onset of ra and spa. indeed, as bone marrow stem cell niches are enriched in tregs and myeloid derived suppressor cells, these areas are favourable for the persistence of quiescent viruses and/or dormant bacteria. this review focuses on the possibility that such silent infections of bone marrow stem cell niches might contribute to the pa ... | 2017 | 26886813 |
| tropheryma whipplei infection and whipple's disease. | recent advances in medical microbiology, epidemiology, cellular biology, and the availability of an expanded set of diagnostic methods such as histopathology, immunohistochemistry, pcr, and bacterial culture have improved our understanding of the clinical range and natural course of tropheryma whipplei infection and whipple's disease. interdisciplinary and transnational research activities have contributed to the clarification of the pathogenesis of the disorder and have enabled controlled trial ... | 2016 | 26856775 |
| short article: relapsing whipple's disease: a case report and literature review. | whipple's disease is a rare infection caused by tropheryma whipplei, a gram-negative bacillus usually found in macrophages of the lamina propria of the small intestine. the typical clinical manifestations of classic whipple's disease are diarrhea, weight loss, malabsorption, abdominal pain, and arthralgia. the disease's laboratory diagnosis is currently based on duodenal biopsy. treatment generally includes primary therapy for 2 weeks with intravenous antibiotics capable of reaching high levels ... | 2016 | 26649803 |
| airway dysbiosis: haemophilus influenzae and tropheryma in poorly controlled asthma. | asthma is a chronic inflammatory disorder of the airways where bacteria may act as protagonists of chronic inflammation. little is known about the relation of airway inflammation to the presence of specific bacterial taxa. we sought to describe the sputum microbiome in adults with poorly controlled asthma.dna was extracted from induced sputum and microbial communities were profiled using 16s rrna pyrosequencing. bacterial species were characterised, and the relationship between microbial populat ... | 2016 | 26647445 |
| high prevalence of asymptomatic carriers of tropheryma whipplei in different populations from the north of spain. | tropheryma whipplei is the causative agent of whipple disease. t. whipplei has also been detected in asymptomatic carriers with a very different prevalence. to date, in spain, there are no data regarding the prevalence of t. whipplei in a healthy population or in hiv-positive patients, or in chronic fatigue syndrome (cfs). therefore, the aim of this work was to assess the prevalence of t. whipplei in stools in those populations. | 2017 | 26585816 |
| tropheryma whipplei bivalvular endocarditis and polyarthralgia: a case report. | tropheryma whipplei infection should be considered in patients with suspected infective endocarditis with negative blood cultures. the case (i) shows how previous symptoms can contribute to the diagnosis of this illness, and (ii) elucidates current recommended diagnostic and therapeutic approaches to whipple's disease. | 2015 | 26577283 |
| [whipple's disease, when to think about it?]. | whipple's disease is a chronic, systemic, bacterial infection caused by tropheryma whipplei. its cardinal symptoms include intermittent and recurrent arthralgia or arthritis together with chronic diarrhoea, abdominal pain and weight loss. it may mimick many chronic inflammatory diseases, and the diagnosis remains a challenge. salivary and faecal quantitative pcr for t. whipplei should be ensued, if positive, by an upper endoscopy for duodenal biopsies. the treatment consists of a combination of ... | 2015 | 26502617 |
| tropheryma whipplei in children with diarrhoea in rural ghana. | tropheryma whipplei has been hypothesized to be able to cause diarrhoea, but data from young children are scarce. in this hospital-based case-control study 534 stool samples of children aged between 2 months and 15 years from rural ghana were analysed for the presence of t. whipplei. overall stool prevalence of t. whipplei was high (27.5%). although there was no difference in t. whipplei carriage overall between cases and controls, cases aged between 0 and 12 months carried t. whipplei in their ... | 2016 | 26456475 |
| tropheryma whipplei, a potential commensal detected in individuals undergoing routine colonoscopy. | mucosal biopsy samples from individuals not suspected of having whipple's disease were tested for the presence of tropheryma whipplei. a sensitive and specific real-time pcr assay targeting a sequence present seven times in the t. whipplei genome was used. t. whipplei dna was detected in 2.0 and 3.8% of the patients undergoing gastroduodenoscopy and colonoscopy, respectively, who were tested. | 2015 | 26447117 |
| primary whipple disease of the brain: case report with long-term clinical and mri follow-up. | whipple disease (wd) is a rare systemic disorder caused by the bacteria tropheryma whipplei. in its classic form, it manifests with gastrointestinal problems including diarrhea, abdominal pain, and weight loss. however, various other systems can be affected, including the central nervous system (cns). even more rarely, the cns is primarily affected without gastrointestinal symptoms and with a negative small bowel biopsy. the incidence of primary cns wd is unknown. we report the case of a young f ... | 2015 | 26445540 |
| use of polymerase chain reaction in the diagnosis of whipple's disease. | whipple's disease, a systemic, chronic infectious disease caused by tropheryma whipplei, is extremely rare in asian populations. a correct diagnosis is necessary due to its high mortality rate. unfortunately, patients are apt to be misdiagnosed with connective tissue diseases since they typically present with arthritis or arthralgia. there are three diagnostic tools for whipple's disease using intestinal tissues: 1) periodic acid-schiff (pas)-positive macrophages; 2) electron microscopic observa ... | 2015 | 26390825 |
| whipple's disease. | whipple's disease (wd) is rarely the cause of a malabsorption syndrome. the disease is a chronic infection of the intestinal mucosa with the bacterium tropheryma whipplei, which leads to a lymphostasis with an impaired absorption of the nutrition. due to its low incidence (1:1,000,000) and the non-specific early symptoms, the disease is often diagnosed only after many years. | 2014 | 26288590 |
| arthralgia and blood culture-negative endocarditis in middle age men suggest tropheryma whipplei infection: report of two cases and review of the literature. | whipple's disease is a rare, often multisystemic chronic infectious disease caused by the rod-shaped bacterium tropheryma whipplei. very rarely the heart is involved in the process of the disease, leading to culture-negative infective endocarditis. up to 20 % of all infective endocarditis are blood culture-negative and therefore a diagnostic challenge. we present two unusual cases of culture-negative infective endocarditis encountered in two different patients with prior history of arthralgia. a ... | 2015 | 26282628 |
| tropheryma whipplei endocarditis: a two-patient case series. | whipple endocarditis is caused by tropheryma whipplei and is a well-described complication of whipple's disease. limited and small case series have been published regarding the presentation, diagnosis, and clinical course of this disease. | 2015 | 26239095 |
| whipple's disease diagnosed during anti-tumor necrosis factor alpha treatment: two case reports and review of the literature. | whipple's disease is a rare infectious disease caused by tropheryma whipplei with protean clinical manifestations. this infection may mimic chronic inflammatory rheumatisms. | 2015 | 26215452 |
| central nervous system involvement as relapse in undiagnosed whipple's disease with atypical symptoms at onset. | whipple's disease (wd) is a rare systemic disease caused by the gram-positive bacillus tropheryma whipplei and mostly characterized by arthralgias, chronic diarrhea, weight loss, fever and abdominal pain. central nervous system involvement is not uncommon and it may precede other disease manifestations, appear after treatment and improvement of gastrointestinal signs or rarely be the only wd symptom. we report a case in a middle-aged male with unexplained neurological signs and symptoms which we ... | 2015 | 26191089 |
| atypical diagnosis diagnosis by endoscopic capsule: whipple's disease. | whipple's disease is a chronic systemic infection produced by the actinomycete tropheryma whipplei. endoscopic tests are key in the diagnosis as they allow biopsy and histopathological examination for definitive diagnosis of this entity. we present a case of whipple's disease where capsule endoscopy, uncommon for the diagnosis of this condition, was essential for it and its performance before and after antibiotic treatment allows to describe the macroscopic evolution of the findings in the small ... | 2016 | 26182245 |
| -295 t-to-c promoter region il-16 gene polymorphism is associated with whipple's disease. | whipple's disease (wd) is a rare systemic condition caused, in genetically predisposed subjects, by tropheryma whipplei, a common bacterium widespread in the environment. the relevance of genetic predisposition in wd is shown by the association with hla alleles drb1*13 and dqb1*06 and by the demonstration that, in patients with wd, the cytokine genetic profile is skewed toward a th2 and treg response. since il-16 is involved in hampering the development of a protective macrophagic response again ... | 2015 | 26154181 |
| [whipple's disease manifested as difficult-to-diagnose polyarthralgia: a case report and literature review]. | whipple's disease is a rare and multisystemic bacterial disease caused by the gram-positive bacillus tropheryma whipplei. since it is a rare disease with a varied clinical presentation, its diagnosis is often delayed. the joint manifestations may precede the typical syndrome in decades, being a challenge for specialists and internists. we report a case of whipple's disease in a man with polyarthralgia whose diagnosis was established after extensive clinical deterioration over five years. | 2015 | 26066295 |
| rapidly progressive dementia with false-positive pcr tropheryma whipplei in csf. a case of hashimoto's encephalopathy. | 2015 | 26050522 | |
| tropheryma whipplei , immunosuppression and whipple's disease: from a low-pathogenic, environmental infectious organism to a rare, multifaceted inflammatory complex. | the actinobacterium tropheryma whipplei was detected 20 years ago by molecular techniques, and following its culture has been characterized as the cause of a systemic infection known as whipple's disease (wd). t. whipplei occurs in the environment, is prevalent only in humans, is believed to be transmitted via oral routes and to be host dependent. | 2015 | 25925922 |
| sewage workers with low antibody responses may be colonized successively by several tropheryma whipplei strains. | asymptomatic faecal carriage of tropheryma whipplei, the agent of whipple's disease, is reported among sewage workers. however, the potential development of such carriage is unknown. a 7-year follow-up of t. whipplei-carrying sewage workers is reported. | 2015 | 25910853 |
| positron emission tomography in the diagnosis of whipple's endocarditis: a case report. | whipple's disease is a systemic infection that sometimes is associated with cardiac manifestations. the diagnosis of tropheryma whipplei endocarditis is still the result of chance because there are no diagnostic criteria and clinical signs are often those of cardiac disease rather than infection. | 2015 | 25889155 |
| gastrointestinal diagnosis of classical whipple disease: clinical, endoscopic, and histopathologic features in 191 patients. | classic whipple disease (cwd) is a systemic infection caused by tropheryma whipplei. different diagnostic tools have been developed over the last decades: periodic acid-schiff (pas) staining, t whipplei-specific polymerase chain reaction (pcr), and t whipplei-specific immunohistochemistry (ihc). despite all these advances, cwd is still difficult to diagnose because of a variety of clinical symptoms and possibly a long time span between first unspecific symptoms and the full-blown clinical pictur ... | 2015 | 25881849 |
| prevalence of whipple's disease in north-western italy. | whipple's disease (wd) is a rare systemic infection due, in genetically susceptible individuals, to tropheryma whipplei, a heterogeneous gram-positive actinobacteria. although it has already been recognised that wd affects mainly middle-aged caucasian men, the prevalence of wd is virtually unknown. the annual incidence of wd in the general population is said to be less than 1 per 1,000,000, but scientific evidence for these figures is still lacking. on the basis of the number of patients recorde ... | 2015 | 25804189 |
| [tropheryma whipplei: pathogen of whipple's disease and more]. | within the last years the understanding of infection with tropheryma whipplei was significantly enhanced by improvement of molecular biology, microbiology and immunology. the following entities of infection or carriage, respectively, with t. whipplei must be differentiated: besides in the context of classical whipple's disease (cwd), the rare chronic infection with t. whipplei (estimated incidence: 1 : 1,000,000), t. whipplei can be detected more frequently in stool specimens of children with ac ... | 2015 | 25774735 |
| high prevalence of tropheryma whipplei in lao kindergarten children. | tropheryma whipplei is a bacterium commonly found in feces of young children in africa, but with no data from asia. we estimated the prevalence of t. whipplei carriage in feces of children in lao pdr (laos). | 2015 | 25699514 |
| systematic review: whipple's disease (tropheryma whipplei infection) and its unmasking by tumour necrosis factor inhibitors. | the classical form of whipple's disease (wd), clinically characterised by arthropathy, diarrhoea and weight loss, is rare. recently, other more frequent forms of tropheryma whipplei infection have been recognised. the clinical spectrum includes an acute, self-limiting disease in children, localised forms affecting cardiac valves or the central nervous system without intestinal symptoms, and asymptomatic carriage of t. whipplei which is found in around 4% of europeans. genomic analysis has shown ... | 2015 | 25693648 |
| case 12--2015: tropheryma whipplei endocarditis. | 2015 | 25675891 | |
| tropheryma whipplei as a causative agent of travelers' diarrhea: further studies required. reply to razavi sm. | 2015 | 25614092 | |
| chronic infections of the small intestine. | chronic infections of the small intestine cause significant morbidity and mortality globally. this review focuses on the recent advances in the field of our understanding of selected intestinal infections. | 2015 | 25603404 |
| tropheryma whipplei as a causative agent of travelers' diarrhea: further studies required. | 2015 | 25575831 | |
| current and past strategies for bacterial culture in clinical microbiology. | a pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. the first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. the rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. th ... | 2015 | 25567228 |
| immune reconstitution inflammatory syndrome during treatment of whipple's disease. | immune reconstitution inflammatory syndrome is a rare complication of the treatment of whipple's disease. here, we report the case of a 65-year-old man treated for whipple's disease affecting the joints, with positive tropheryma whipplei pcr in csf, who developed fever and nodular eruption on the trunk, arms and face in association with biological inflammatory syndrome 10 days after initiation of antimicrobial treatment. skin manifestations and the patient's general condition improved on cortico ... | 2015 | 25553832 |
| [diagnostic approach of intracellular bacteria and fastidious microorganisms]. | obligate or facultative intracellular bacteria are fastidious organisms that do not or poorly grow on conventional culture media. some of them may be the cause of frequent and potentially severe infections, such as tuberculosis (myco- bacterium tuberculosis), community-acquired respiratory infections (legionella spp., mycoplasma pneumoniae, chlamydia pneumoniae) or blood culture-negative endocarditis (coxiella burnetii, bartonella spp., tropheryma whipplei). the objective of this paper is to pro ... | 2014 | 25549373 |
| complicated whipple's disease and endocarditis following tumor necrosis factor inhibitors. | to test whether treatment with tumor necrosis factor inhibitors (tnfi) is associated with complications of tropheryma whipplei (t. whipplei) infection. | 0 | 25548618 |
| [whipple's disease in an elderly patient with a substantial weight loss and gastrointestinal symptoms]. | whipple's disease is a multisystemic infection with tropheryma whipplei often characterised by its gastrointestinal involvement. however, due to the broad spectrum of unspecific symptoms, the diagnosis is often delayed. we report a case of a 70 year-old-man, who presented with extensive weight loss and lymphadenopathy. the diagnosis of whipple's disease was made 18 months later on a duodenal biopsy. retrospectively, it was discovered that a mesenterial biopsy taken earlier also revealed signs of ... | 2014 | 25497640 |
| update on blood culture-negative endocarditis. | blood culture-negative endocarditis is often severe, and difficult to diagnose. the rate of non-documented infective endocarditis has decreased with the advent of molecular biology - improved performance for the diagnosis of bacterial endocarditis with blood cultures sterilized by previous antibacterial treatment - and cardiac surgery - access to the main infected focus, the endocardium, for half of the patients. blood culture-negative endocarditis are classified in 3 main categories: (i) bacter ... | 2015 | 25480453 |
| relapse of tropheryma whipplei endocarditis treated by trimethoprim/sulfamethoxazole, cured by hydroxychloroquine plus doxycycline. | the best treatment for tropheryma whipplei infections is controversial. we report a patient who suffered from t. whipplei aortic native valve endocarditis that relapsed despite surgery and four weeks of intravenous ceftriaxone followed by several months of oral trimethoprim/sulfamethoxazole. cure was achieved after replacement of the prosthesis with a homograft and 18 months of oral doxycycline-hydroxychloroquine. we discuss the need for a change in treatment guidelines for t. whipplei infection ... | 2015 | 25461667 |
| gorilla gorilla gorilla gut: a potential reservoir of pathogenic bacteria as revealed using culturomics and molecular tools. | wild apes are considered to be the most serious reservoir and source of zoonoses. however, little data are available about the gut microbiota and pathogenic bacteria in gorillas. for this propose, a total of 48 fecal samples obtained from 21 gorilla gorilla gorilla individuals (as revealed via microsatellite analysis) were screened for human bacterial pathogens using culturomics and molecular techniques. by applying culturomics to one index gorilla and using specific media supplemented by plants ... | 2014 | 25417711 |
| role of dendritic cells in the pathogenesis of whipple's disease. | accumulation of tropheryma whipplei-stuffed macrophages in the duodenum, impaired t. whipplei-specific th1 responses, and weak secretion of interleukin-12 (il-12) are hallmarks of classical whipple's disease (cwd). this study addresses dendritic cell (dc) functionality during cwd. we documented composition, distribution, and functionality of dc ex vivo or after in vitro maturation by fluorescence-activated cell sorting (facs) and by immunohistochemistry in situ. a decrease in peripheral dc of un ... | 2014 | 25385798 |
| [whipple's disease]. | whipple's disease (wd) is a rare, chronic, systemic infection caused by the bacterium tropheryma whipplei. new molecular techniques and epidemiological data over the latest decade have contributed to better understanding of this infection. the classical form of wd is characterized by arthritis followed years after by diarrhoea, weight loss and malabsorption but other clinical forms without intestinal involvement have been described. prompt recognition and treatment of the infection is important, ... | 2014 | 25346310 |
| atypical focal forms of whipple's disease seen by rheumatologists. | we report two atypical cases of focal whipple's disease with rheumatic presenting symptoms. in one of these cases, the patient presented with chronic intermittent polyarthritis, systemic inflammation, and leukocytosis. tests were positive for rheumatoid factor and anti-cyclic citrullinated peptide antibodies. there was no structural joint damage. combined glucocorticoid and methotrexate therapy was only partially effective. endocarditis requiring emergency valve replacement surgery occurred 4 ye ... | 2015 | 25311252 |
| whipple's disease in an afro-caribbean national. | whipple's disease is a rare multi-organ infectious disease caused by tropheryma whipplei. it is fatal without treatment. we report on a 40-year old afro-jamaican man who presented with a six-month history of weight loss and diarrhoea. investigations revealed iron deficiency anaemia and hypoalbuminaemia. upper gastrointestinal endoscopy revealed white patchy lesions in the duodenum. the duodenal biopsy showed broadening and thickening of the villi by a dense infiltrate of foamy histiocytes within ... | 2014 | 25303201 |
| a case of whipple's disease evolving over 30 years. | 2014 | 25015310 | |
| whipple's disease concomitant with candida esophagitis and subsequent giardia lamblia coinfection. | whipple's disease is a rare systemic condition resulting from a chronic infection by tropheryma whipplei. clinical presentation can be widely heterogeneous, often leading to delayed diagnosis and treatment. furthermore, little is known about the underlying pathogenic mechanisms, although several abnormalities in immune cell function have been observed. we describe the case of a 51-year-old woman presenting with dysphagia, odynophagia, long-lasting low-grade fever, and malabsorption syndrome who ... | 2014 | 25014626 |
| isolated whipple's endocarditis: an underestimated diagnosis that requires molecular analysis of surgical material. | tropheryma whipplei is known as the bacterium that causes whipple's disease, a rare systemic illness typically affecting gastrointestinal tract, joints, and central nervous system. in addition, t whipplei infection may present as an isolated endocarditis. although previously regarded as a rare condition, t whipplei has been recognized as a major cause of culture-negative endocarditis when integrating specific molecular analysis of surgical material into the diagnostic algorithm. here, we report ... | 2014 | 24996742 |
| tropheryma whipplei endocarditis: the value of valve polymerase chain reaction in cardiac surgery. | 2014 | 24951019 | |
| prevalence and risk factor assessment of tropheryma whipplei in a rural community in gabon: a community-based cross-sectional study. | tropheryma whipplei is the causative agent of whipple's disease and has been detected in stools of asymptomatic carriers. colonization has been associated with precarious hygienic conditions. there is a lack of knowledge about the epidemiology and transmission characteristics on a population level, so the aim of this study was to determine the overall and age-specific prevalence of t. whipplei and to identify risk factors for colonization. this molecular epidemiological survey was designed as a ... | 2014 | 24943959 |
| [whipple's disease and tropheryma whipplei infections in internal medicine. when to think about it? how to treat?]. | tropheryma whipplei culture and genome sequencing have, thanks to new tools, rationalized both diagnosis and treatment of whipple's disease. whipple's disease involves mainly caucasian male, approximately 50-years-old, suffering from arthralgia, weight loss and diarrhea. a worsening of clinical manifestations after immunosuppressive therapy is frequently observed while antibiotics prescribed for another infection improves the clinical status. clinical manifestations may more rarely suggest lymph ... | 2014 | 24933290 |
| service evaluation to establish the sensitivity, specificity and additional value of broad-range 16s rdna pcr for the diagnosis of infective endocarditis from resected endocardial material in patients from eight uk and ireland hospitals. | infective endocarditis (ie) can be diagnosed in the clinical microbiology laboratory by culturing explanted heart valve material. we present a service evaluation that examines the sensitivity and specificity of a broad-range 16s rdna polymerase chain reaction (pcr) assay for the detection of the causative microbe in culture-proven and culture-negative cases of ie. a clinical case-note review was performed for 151 patients, from eight uk and ireland hospitals, whose endocardial specimens were ref ... | 2014 | 24930039 |
| whipple's disease. | whipple's disease is a rare chronic multi-systemic infection, caused by gram-positive bacillus tropheryma whipplei. the infection usually involves the small bowel, but other organs may also be involved. the diagnosis is often challenging and can only be made on histopathological examination. this report describes 2 patients presenting with abdominal pain and weight loss who finally were diagnosed to have whipple's disease. one of the patients was a renal transplant recipient. to the best of auth ... | 2014 | 24906280 |
| tropheryma whipplei and whipple's disease. | since its identification, information concerning tropheryma whipplei, the causative agent of whipple's disease (wd), has increased. although t. whipplei is commonly infecting humans, wd is rare. the bacterium is most likely transmitted among humans via the oro-oral and the feco-oral routes. infections result in chronic conditions such as asymptomatic carriage, disseminated whipple's disease, which is usually preceded by arthralgias, and localized infections, such as endocarditis or encephalitis. ... | 2014 | 24877762 |
| occurrence of tropheryma whipplei during diarrhea in hajj pilgrims: a pcr analysis of paired rectal swabs. | tropheryma whipplei was recently associated with gastroenteritis in children. we hypothesize that t. whipplei may be a contributing microbe in traveller's diarrhea. | 2014 | 24797353 |
| an unusual cause of pancytopenia: whipple's disease. | whipple's disease is a systemic infectious disease caused by the bacteria tropheryma whipplei. the most common clinical manifestations of whipple's disease are weight loss (92%), hypoalbuminemia and steatorrhea (91%, respectively), diarrhea (72%), arthralgia (67%), and abdominal pain (55%). neurological signs and symptoms from dementia to oculomasticatory myorhythmia or oculofacioskeletal myorhythmia (pathognomonic of whipple's disease), lymphadenopathy, and fatigue can also be present. pancytop ... | 2014 | 24765256 |
| tumoural form of whipple's disease simulating carcinomatosis. | whipple's disease is a rare disease caused by the actinomycete bacteria tropheryma whipplei, which cause intestinal infection. the most common symptoms are chronic diarrhoea, weight loss, abdominal pain, arthritis and neurological abnormalities, which can be fatal. this paper reports a case of a 57-year-old brazilian woman with diarrhoea, vomiting, abdominal pain, appetite loss, intermittent fever, malaise, weight loss and malnutrition. migratory polyarthralgia and recurrent visual scotomas prec ... | 2014 | 24690429 |
| does tropheryma whipplei contribute to travelers' diarrhea?: a pcr analysis of paired stool samples in french travelers to senegal. | tropheryma whipplei was recently associated with acute infections as gastroenteritis in children. we hypothesize that t. whipplei may be a contributing microbe in traveler's diarrhea. | 2015 | 24630594 |
| tropheryma whipplei natural resistance to trimethoprim and sulphonamides in vitro. | 2014 | 24613421 | |
| tropheryma whipplei, the agent of whipple's disease, affects the early to late phagosome transition and survives in a rab5- and rab7-positive compartment. | tropheryma whipplei, the agent of whipple's disease, inhibits phago-lysosome biogenesis to create a suitable niche for its survival and replication in macrophages. to understand the mechanism by which it subverts phagosome maturation, we used biochemical and cell biological approaches to purify and characterise the intracellular compartment where tropheryma whipplei resides using mouse bone-marrow-derived macrophages. we showed that in addition to lamp-1, the tropheryma whipplei phagosome is pos ... | 2014 | 24586722 |
| specific cutaneous involvement in whipple disease. | cutaneous lesions in whipple disease (wd) are infrequent, and the histological findings are usually nonspecific. specific cutaneous lesions have rarely been described and usually involve the subcutaneous fat. we report a patient diagnosed with wd, who developed multiple small subcutaneous nodules after antibiotic treatment was administered. in addition to septal panniculitis, the cutaneous biopsy showed a mild granulomatous dermal reaction with pas-positive macrophages characteristic of wd. a po ... | 2014 | 24562051 |
| immune reconstitution inflammatory syndrome associated with bacterial infections. | immune reconstitution inflammatory syndrome (iris) is defined by various clinical manifestations following the initiation of antiretroviral treatment (art) in hiv patients primarily infected with mycobacteria. iris has clinical similarities with lepromatous reactions in patients with leprosy following antibiotic initiation. | 2014 | 24512544 |
| bacterial lymphadenitis at a major referral hospital in france from 2008 to 2012. | lymph node enlargement is a common medical problem, and in a large number of patients, the causes of lymphadenopathy remain undiagnosed. we report a thorough microbiological analysis of 1,688 lymph node biopsy specimens collected in our bartonellosis reference center. we studied lymph node biopsy samples from patients with suspected regional infectious lymph node enlargement from january 2008 to december 2012. to evaluate a useful strategy for the diagnosis of infectious lymphadenitis, specimens ... | 2014 | 24478415 |
| lung microbiome for clinicians. new discoveries about bugs in healthy and diseased lungs. | microbes are readily cultured from epithelial surfaces of the skin, mouth, and colon. in the last 10 years, culture-independent dna-based techniques demonstrated that much more complex microbial communities reside on most epithelial surfaces; this includes the lower airways, where bacterial culture had failed to reliably demonstrate resident bacteria. exposure to a diverse bacterial environment is important for adequate immunological development. the most common microbes found in the lower airwa ... | 2014 | 24460444 |
| whipple's in the valleys: a case of whipple's with thrombocytopenia and endocarditis. | this case report details the second described case of whipple's disease-related thrombocytopenia in the medical literature. whipple's disease is a rare multisystem infection caused by the actinomycete tropheryma whipplei, first described by george whipple in 1907. the key clinical manifestations are weight loss, diarrhoea and malabsorption, but arthralgia and endocarditis are also well described. | 2014 | 24459171 |
| constrictive pleuropericarditis: a dominant clinical manifestation in whipple's disease. | whipple's disease is a rare, multisystemic, chronic infectious disease which classically presents as a wasting illness characterized by polyarthralgia, diarrhea, fever, and lymphadenopathy. pleuropericardial involvement is a common pathologic finding in patients with whipple's disease, but rarely causes clinical symptoms. we report the first case of severe fibrosing pleuropericarditis necessitating pleural decortication in a patient with whipple's disease. | 2013 | 24321135 |
| tropheryma whipplei endocarditis. | tropheryma whipplei endocarditis differs from classic whipple disease, which primarily affects the gastrointestinal system. we diagnosed 28 cases of t. whipplei endocarditis in marseille, france, and compared them with cases reported in the literature. specimens were analyzed mostly by molecular and histologic techniques. duke criteria were ineffective for diagnosis before heart valve analysis. the disease occurred in men 40-80 years of age, of whom 21 (75%) had arthralgia (75%); 9 (32%) had val ... | 2013 | 24207100 |
| rheumatic and musculoskeletal features of whipple disease: a report of 29 cases. | whipple disease is a rare infection caused by tropheryma whipplei. although patients commonly complain of osteoarticular involvement, musculoskeletal manifestations have been poorly described. we report cases of whipple disease with rheumatic symptoms and describe their clinical presentation, modes of diagnosis, and outcomes. | 2013 | 24187107 |
| central nervous system involvement in whipple disease: clinical study of 18 patients and long-term follow-up. | whipple disease (wd) is a rare multisystemic infection with a protean clinical presentation. the central nervous system (cns) is involved in 3 situations: cns involvement in classic wd, cns relapse in previously treated wd, and isolated cns infection. we retrospectively analyzed clinical features, diagnostic workup, brain imaging, cerebrospinal fluid (csf) study, treatment, and follow-up data in 18 patients with wd and cns infection. ten men and 8 women were included with a median age at diagnos ... | 2013 | 24145700 |
| whipple's disease. | whipple's disease is a chronic, systemic infection caused by tropheryma whipplei. gene amplification, isolation and dna sequencing of t whipplei have extended our knowledge of this pathogen, which is now recognised as a ubiquitous commensal bacterium. the spectrum of signs associated with t whipplei has now been extended beyond the classic form, which affects middle-aged men, and begins with recurrent arthritis followed several years later by digestive problems associated with other diverse clin ... | 2013 | 24129033 |
| tropheryma whipplei pneumonia in a patient with hiv-2 infection. | 2013 | 24127807 | |
| [weight loss, dementia and ataxia susceptible to doxycycline: a likely new case report caused by t. whipplei]. | whipple's disease is a rare infectious disease due to tropheryma whipplei, a bacterium rarely causing severe localized neurological infection (only 25 cases reported in the literature), which are more often diagnosed by a positive t. whipplei pcr performed on cerebrospinal fluid. | 2013 | 24075626 |
| validation of an rpob gene pcr assay for detection of tropheryma whipplei: 10 years' experience in a national reference laboratory. | the performance of a real-time pcr assay targeting the tropheryma whipplei rpob gene was evaluated using test strains and 1,236 clinical specimens in a national reference laboratory. the novel rpob-pcr assay proved to be specific, revealed improved analytical sensitivity, and substantially accelerated detection of t. whipplei dna in clinical specimens. | 2013 | 23966507 |
| treatment of classic whipple's disease: from in vitro results to clinical outcome. | patients with classic whipple's disease have a lifetime defect in immunity to tropheryma whipplei and frequently develop treatment failures, relapses or reinfections. empirical treatments were tested before culture was possible, but the only in vitro bactericidal treatment consists of a combination of doxycycline and hydroxychloroquine. | 2014 | 23946319 |
| [duodenal biopsy is the most important examination when diagnosing whipple's disease]. | whipple's disease is a rare disease caused by the bacteria tropheryma whipplei. it can present with different disease patterns including arthritis, gastrointestinal symptoms with abdominal pain and diarrhoea, malnutrition, anaemia and neurological symptoms. we present a case of a 71-year-old man who underwent a six-month long, thorough investigation before reaching the right diagnosis. after sufficient treatment the symptoms remitted. physicians should be vigilant of this differential diagnosis ... | 2013 | 23937876 |
| [drug therapy of infectious diarrhea. part 2: chronic diarrhea]. | diarrheal diseases are among the most common diseases worldwide. in this review the current treatment recommendations for acute (part 1) and chronic (part 2) infectious diarrhea are summarized and typical enteropathogens are discussed. the second part of the article describes chronic diarrhea, its related pathogens and treatment. in contrast to acute diarrhea which is mainly caused by viral and typical bacterial pathogens, chronic diarrhea has mainly non-infectious origins. protozoal pathogens, ... | 2013 | 23917963 |