Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| bioresolution of racemic phenyl glycidyl ether by a putative recombinant epoxide hydrolase from streptomyces griseus nbrc 13350. | in order to produce enantiomerically pure epoxides for the synthesis of value-added chemicals, a novel putative epoxide hydrolase (eh) sgeh was cloned and overexpressed in pet28a/escherichia coli bl21(de3). the 1047 bp sgeh gene was mined from streptomyces griseus nbrc 13350 genome sequence. the recombinant hexahistidyl-tagged sgeh was purified (16.6-fold) by immobilized metal-affinity chromatography, with 90% yield as a homodimer of 100 kda. the recombinant e. coli whole cells overexpressing sg ... | 2017 | 28378221 |
| characterization of bafilomycin biosynthesis in kitasatospora setae km-6054 and comparative analysis of gene clusters in actinomycetales microorganisms. | bafilomycins a1, c1 and b1 (setamycin) produced by kitasatospora setae km-6054 belong to the plecomacrolide family, which exhibit antibacterial, antifungal, antineoplastic and immunosuppressive activities. an analysis of gene clusters from k. setae km-6054 governing the biosynthesis of bafilomycins revealed that it contains five large open reading frames (orfs) encoding the multifunctional polypeptides of bafilomycin polyketide synthases (pkss). these clustered pks genes, which are responsible f ... | 2017 | 28293034 |
| 79 the cell-cycle related nuclear localization of platelet-activating factor in bovine and murine embryo development. | platelet-activating factor (paf) is a lipid mediator produced by a wide variety of cells and well known for its involvement in host defense and platelet aggregation. in embryo development, paf functions as an embryotropin and a marker of embryo quality and viability in human and mice. however, only limited information is available concerning its function during in vitro culture of bovine embryos. bovine and murine embryos were collected at different time points according to their development (4- ... | 2016 | 28278876 |
| β-glucosidase from streptomyces griseus: nanoparticle immobilisation and application to alkyl glucoside synthesis. | a novel β-glucosidase from streptomyces griseus was cloned and overexpressed in e. coli. the purified β-glucosidase (44 kda) had a km of 8.6 ± 0.5 mm and a vmax of 217 ± 5.0 μmoles(-1)min(-1)mg at 37 °c, ph 7.2 with p-nitrophenyl-β-d glucopyranoside as substrate. the enzyme was characterised in terms of ph optimum (ph 6.9), temperature optimum (69 °c) and the influence of solvents and effectors. purified s. griseus β-glucosidase was successfully immobilised, by simple absorption, onto zinc oxide ... | 2017 | 28161545 |
| protein acetylation involved in streptomycin biosynthesis in streptomyces griseus. | protein acetylation, the reversible addition of an acetyl group to lysine residues, is a protein post-translational modification ubiquitous in living cells. although the involvement of protein acetylation in the regulation of primary metabolism has been revealed, the function of protein acetylation is largely unknown in secondary metabolism. here, we characterized protein acetylation in streptomyces griseus, a streptomycin producer. protein acetylation was induced in the stationary and sporulati ... | 2017 | 28034645 |
| new and highly active microbial phosphotriesterase sources. | many toxic insecticides used worldwide as well as some chemical warfare agents are phosphotriester derivatives. therefore, detoxification of organophosphorus compounds has become the subject of many studies and in particular bioremediation, based on the phosphotriesterase catalysed hydrolysis of these compounds, has shown to be an effective and ecological methodology. in order to identify new bacterial phosphotriesterases, a simple and sensitive fluorimetric screening method on solid media was e ... | 2016 | 27915249 |
| complete genome sequence of the streptomyces phage nanodon. | streptomyces phage nanodon is a temperate double-stranded dna siphoviridae belonging to cluster bd1. it was isolated from soil collected in kilauea, hi, using streptomyces griseus subsp. griseus as a host. | 2016 | 27795236 |
| biosynthesis of optically pure chiral alcohols by a substrate coupled and biphasic system with a short-chain dehydrogenase from streptomyces griseus. | the increasing demand for biocatalysts in synthesizing enantiomerically pure chiral alcohols results from the outstanding characteristics of enzymes in reaction, economic, ecological issues. many carbonyl reductases for producing chiral alcohols have been reported but there is still a lack of good catalytic efficacies. herein, five carbonyl reductases from different streptomyces were discovered by the strategy of genome mining. these reductases were overexpressed, and we chose sgcr for further s ... | 2016 | 27702481 |
| high production of a class iii lantipeptide amfs in streptomyces griseus. | amfs, a class iii lantipeptide serves as a morphogen in streptomyces griseus. here, we constructed a high production system of amfs in s. griseus. we isolated s. griseus grd1 strain defective in glucose repression of aerial mycelium formation and found it suitable for the overproduction of amfs. two expression vectors carrying the strong and constitutive erme2 promoter were constructed using a multicopy number plasmid, pij702. the use of the grd1 strain combined with the expression vectors enabl ... | 2017 | 27691921 |
| production of n-acetyl-d-glucosamine from mycelial waste by a combination of bacterial chitinases and an insect n-acetyl-d-glucosaminidase. | n-acetyl-d-glucosamine (glcnac) has great potential to be used as a food additive and medicine. the enzymatic degradation of chitin-containing biomass for producing glcnac is an eco-friendly approach but suffers from a high cost. the economical efficiency can be improved by both optimizing the member and ratio of the chitinolytic enzymes and using new inexpensive substrates. to address this, a novel combination of bacterial and insect chitinolytic enzymes was developed in this study to efficient ... | 2016 | 27546481 |
| isolation and evaluation of proteolytic actinomycete isolates as novel inducers of pearl millet downy mildew disease protection. | native endophytic actinomycetes isolated from pearl millet roots were examined for their efficacy to protect pearl millet against downy mildew. nineteen of 39 isolates were found to be proteolytic, of which 7 strains could directly suppress the sporangium formation of sclerospora graminicola, the pearl millet downy mildew pathogen. thus, mycelial suspensions containing either spores or cell-free extract of these 7 isolates were used for seed-coating and -soaking treatments to test for their indu ... | 2016 | 27499196 |
| efficient production of nonactin by streptomyces griseus subsp. griseus. | here we report the production of the cyclic macrotetrolide nonactin from the fermentation culture of streptomyces griseus subsp. griseus. nonactin is a member of a family of naturally occurring cyclic ionophores known as the macrotetrolide antibiotics. our fermentation procedure of streptomyces griseus was performed at 30 °c and 200 rev·min(-1) for 5 days on a rotary shaker. diaion hp-20 and amberlite xad-16 were added to the fermentation medium. isolated yield of nonactin was up to 80 mg·l(-1) ... | 2016 | 27405846 |
| the production of flocculating substance(s) by streptomyces griseus. | streptomyces griseus produced a certain kind of flocculating substance(s). the flocculating substance(s) could aggregate suspended kaolin particles in solution to form small floes and enables it to sediment those particles. the production of flocculating substance(s) by s. griseus was not parallel to the cell growth and a large number of flocculating substance(s) was released into the culture broth in the death phase. the flocculating substance(s) produced by s. griseus acted under acidic condit ... | 1996 | 27299553 |
| effects of organic solvents on streptomyces griseus protease i with transfer action. | the protease i from streptomyces griseus var. alcalophilus strongly catalyzed the transfer reaction forming hydroxamic acids of various amino acids, especially aromatic and hydrophobic amino acids. dimethyl sulfoxide (dmso), 1,4-butane diol, and glycerol protected this enzyme from heat denaturation. moreover, only dmso strongly activated the transfer action of protease i and the velocity of reaction was accelerated three times in the presence of 50% dmso. the yield of transfer product strongly d ... | 1992 | 27280814 |
| an abc transporter involved in the control of streptomycin production in streptomyces griseus. | we screened for a gene that inhibits streptomycin production in streptomyces griseus when it is introduced on a high-copy-number plasmid pij702, and obtained a plasmid pkm545. the introduction of pkm545 abolished streptomycin production on all media tested including ymp-sugar and nutrient broth. s1 protection analysis demonstrated that the introduction of this plasmid downregulated the transcriptional activity of the promoter preceding strr, the pathway-specific transcriptional regulator for str ... | 2016 | 27268270 |
| evaluation of antagonistic and plant growth promoting activities of chitinolytic endophytic actinomycetes associated with medicinal plants against sclerotium rolfsii in chickpea. | to evaluate the potential of chitinolytic endophytic actinomycetes isolated from medicinal plants in order to diminish the collar rot infestation induced by sclerotium rolfsii in chickpea. | 2016 | 27170067 |
| marine-derived secondary metabolite, griseusrazin a, suppresses inflammation through heme oxygenase-1 induction in activated raw264.7 macrophages. | a new secondary metabolite, named griseusrazin a (1), was isolated from the marine-derived bacterium streptomyces griseus subsp. griseus. the structure of the compound was determined by analysis of spectroscopic data including ms, cosy, hsqc, hmbc, and (15)n-hmbc data. griseusrazin a (1) inhibited the production of inflammatory mediators, such as prostaglandin e2 and nitric oxide, which was mediated through the suppression of the expression of cyclooxygenase-2 (cox-2) and inducible nitric oxide ... | 2016 | 27019105 |
| improved production of active streptomyces griseus trypsin with a novel auto-catalyzed strategy. | n-terminal sequences play crucial roles in regulating expression, translation, activation and enzymatic properties of proteins. to reduce cell toxicity of intracellular trypsin and increase secretory expression, we developed a novel auto-catalyzed strategy to produce recombinant trypsin by engineering the n-terminus of mature streptomyces griseus trypsin (sgt). the engineered n-terminal peptide of sgt was composed of the thioredoxin, glycine-serine linker, his6-tag and the partial bovine trypsin ... | 2016 | 26983398 |
| grisemycin, a bridged angucyclinone with a methylsulfinyl moiety from a marine-derived streptomyces sp. | grisemycin (1), the first sulfur angucyclinone with an unusual ether-bridged system, was isolated from a marine-derived streptomyces griseus strain m268. its novel, here cage-like, structure was determined by spectroscopic analysis and single-crystal x-ray diffraction. compound 1 exhibited modestly selective activity against the hl-60 cell line with an ic50 value of 31.54 μm. futhermore, the absolute stereochemistry of kiamycin (2), an 1,12-epoxybenz[a]anthracene, previously obtained from the sa ... | 2016 | 26958869 |
| naturally occurring isoleucyl-trna synthetase without trna-dependent pre-transfer editing. | isoleucyl-trna synthetase (ilers) is unusual among aminoacyl-trna synthetases in having a trna-dependent pre-transfer editing activity. alongside the typical bacterial ilers (such as escherichia coli ilers), some bacteria also have the enzymes (eukaryote-like) that cluster with eukaryotic ilerss and exhibit low sensitivity to the antibiotic mupirocin. our phylogenetic analysis suggests that the iles1 and iles2 genes of contemporary bacteria are the descendants of genes that might have arisen by ... | 2016 | 26921320 |
| treatment of clostridium difficile infection using sq641, a capuramycin analogue, increases post-treatment survival and improves clinical measures of disease in a murine model. | clostridium difficile infection (cdi) is a primary cause of antibiotic-associated diarrhoeal illness. current therapies are insufficient as relapse rates following antibiotic treatment range from 25% for initial treatment to 60% for treatment of recurrence. in this study, we looked at the efficacy of sq641 in a murine model of cdi. sq641 is an analogue of capuramycin, a naturally occurring nucleoside-based compound produced by streptomyces griseus. | 2016 | 26832756 |
| a pyridoxal phosphate-dependent enzyme that oxidizes an unactivated carbon-carbon bond. | pyridoxal 5'-phosphate (plp)-dependent enzymes have wide catalytic versatility but are rarely known for their ability to react with oxygen to catalyze challenging reactions. here, using in vitro reconstitution and kinetic analysis, we report that the indolmycin biosynthetic enzyme ind4, from streptomyces griseus atcc 12648, is an unprecedented o2- and plp-dependent enzyme that carries out a four-electron oxidation of l-arginine, including oxidation of an unactivated carbon-carbon (c-c) bond. we ... | 2016 | 26807714 |
| iminimycin a, the new iminium metabolite produced by streptomyces griseus os-3601. | a new natural product, designated iminimycin a, was isolated from the cultured broth of a streptomycin-producing microbial strain, streptomyces griseus os-3601, via a physicochemical screening method using hp-20, silica gel and ods column chromatographies and subsequent preparative hplc. iminimycin a is an indolizidine alkaloid, containing of an unusual iminium group and a cyclopropane ring with a triene side chain. the absolute configuration of iminimycin a was elucidated by nmr studies and ele ... | 2016 | 26758492 |
| a branch point of streptomyces sulfur amino acid metabolism controls the production of albomycin. | albomycin (abm), also known as grisein, is a sulfur-containing metabolite produced by streptomyces griseus atcc 700974. genes predicted to be involved in the biosynthesis of abm and abm-like molecules are found in the genomes of other actinomycetes. abm has potent antibacterial activity, and as a result, many attempts have been made to develop abm into a drug since the last century. although the productivity of s. griseus can be increased with random mutagenesis methods, understanding of strepto ... | 2015 | 26519385 |
| biofiltration technology for the removal of toluene from polluted air using streptomyces griseus. | biofiltration technology has been recognized as a promising biotechnology for treating the volatile organic compounds (vocs) present in polluted air. this study aims to investigate the performance of a biofiltration system of streptomyces griseus sp. dsm-40759 immobilized on activated carbon (pica s23) towards the adsorption and degradation of toluene vapour as well as to regenerate the activated carbon in situ. the batch studies were performed using nutrient agar medium and basal salt medium (b ... | 2016 | 26469210 |
| antitumor compounds from streptomyces sp. kml-2, isolated from khewra salt mines, pakistan. | actinomycetes are gram positive bacteria with high g + c content in their dna and are capable of producing variety of secondary metabolites. many of these metabolites possess different biological activities and have the potential to be developed as therapeutic agents. the aim of the present study was to screen actinomycetes inhabiting halophilic environment such as khewra salt mines present in pakistan for cytotoxic and antitumor compounds. | 2015 | 26466995 |
| 3-amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant corynebacterium glutamicum. | the production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-ahba) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. recombinant corynebacterium glutamicum strain kt01 expressing grih and grii derived from streptomyces griseus produced 3,4-ahba from the sweet sorghum juice of cultivar sil-05 at a final concentration (1.0 ... | 2015 | 26409852 |
| l-asparaginase from streptomyces griseus niot-vkma29: optimization of process variables using factorial designs and molecular characterization of l-asparaginase gene. | marine actinobacteria are known to be a rich source for novel metabolites with diverse biological activities. in this study, a potential extracellular l-asparaginase was characterised from the streptomyces griseus niot-vkma29. box-behnken based optimization was used to determine the culture medium components to enhance the l-asparaginase production. ph, starch, yeast extract and l-asparagine has a direct correlation for enzyme production with a maximum yield of 56.78 iu ml(-1). a verification ex ... | 2015 | 26206135 |
| enhanced detection of post-transcriptional modifications using a mass-exclusion list strategy for rna modification mapping by lc-ms/ms. | there has been a renewed appreciation for the dynamic nature of ribonucleic acid (rna) modifications and for the impact of modified rnas on organism health resulting in an increased emphasis on developing analytical methods capable of detecting modifications within specific rna sequence contexts. here we demonstrate that a dna-based exclusion list enhances data dependent liquid chromatography tandem mass spectrometry (lc-ms/ms) detection of post-transcriptionally modified nucleosides within spec ... | 2015 | 26176336 |
| a simple and efficient method of slow freezing for human embryonic stem cells and induced pluripotent stem cells. | protocols available for the cryopreservation of human embryonic stem (es) and induced pluripotent stem (ips) cells are very inefficient and laborious compared to those for the cryopreservation of murine es/ips cells or other general cell lines. while the vitrification method may be adequate when working with small numbers of human es/ips cells, it requires special skills and is unsuitable when working with large cell numbers. here, we describe a simple and efficient method for the cryopreservati ... | 2016 | 26069023 |
| estimation of intestinal protein digestibility of protein supplements for ruminants using a three-step enzymatic in vitro procedure. | this study included 33 samples with main focus on unprotected or rumen-protected rapeseed and soybean feedstuffs, which were analysed using an enzymatic in vitro procedure (eivp) in order to determine intestinal crude protein (cp) digestibility (ipd) of ruminally undegraded cp. the eivp involved the sequential digestion of samples with a protease from streptomyces griseus, pepsin-hcl and pancreatin. briefly, the eivp started with determination of true protein. feeds were incubated for 18 h in a ... | 2015 | 26054973 |
| identification of two novel regulatory genes involved in pristinamycin biosynthesis and elucidation of the mechanism for atra-p-mediated regulation in streptomyces pristinaespiralis. | in this study, using a transposon-based strategy, two novel regulatory genes were identified as being involved in the biosynthesis of both pristinamycin i (pi) and ii (pii) in streptomyces pristinaespiralis, including a tetr-family regulatory gene atra-p (ssdg_00466) and an orphan histidine kinase gene ssdg_02492. the mechanism by which atra-p exerted a positive role in pristinamycin production was elucidated. we showed that deletion of atra-p resulted in a delayed production of both pi and pii ... | 2015 | 25957493 |
| systematic identification of a panel of strong constitutive promoters from streptomyces albus. | actinomycetes are important organisms for the biosynthesis of valuable natural products. however, only a limited number of well-characterized native constitutive promoters from actinomycetes are available for the construction and engineering of large biochemical pathways. here, we report the discovery and characterization of 32 candidate promoters identified from streptomyces albus j1074 by rna-seq analysis. these 32 promoters were cloned and characterized using a streptomycete reporter gene, xy ... | 2015 | 25924180 |
| dandamycin and chandrananimycin e, benzoxazines from streptomyces griseus. | two new benzoxazines were isolated from streptomyces griseus (hki 0545) and assigned as chandrananimycin e (1) and dandamycin (2). although a number of phenoxazinone-type compounds have been reported from nature, phenoxazines are rarer, and carbon substitution at n-10 such as in 1 is unprecedented. the cyclopentene-containing ring structure of dandamycin (2) is also unique. chandrananimycin e (1) was found to possess moderate antiproliferative activity against huvec cells (gi50 35.3 μm) and weak ... | 2015 | 25690358 |
| evaluation of nystatin isolated from streptomyces griseus sdx-4 against the ciliate, ichthyophthirius multifiliis. | the present study was conducted to evaluate the in vitro and in vivo antiparasitic efficacy of active compounds from the bacterial extracellular products of streptomyces griseus sdx-4 against ichthyophthirius multifiliis. bioassay-guided fractionation and isolation of compounds with antiparasitic activity were performed on n-butanol extract of s. griseus yielding a pure bioactive compound, nystatin (nys), identified by comparing spectral data (ei-ms, (1)h nmr, and (13)c nmr) with literature valu ... | 2015 | 25645004 |
| differentiations of chitin content and surface morphologies of chitins extracted from male and female grasshopper species. | in this study, we used fourier transform infrared spectroscopy (ft-ir), elemental analysis (ea), thermogravimetric analysis (tga), x-ray diffractometry (xrd), and scanning electron microscopy (sem) to investigate chitin structure isolated from both sexes of four grasshopper species. ft-ir, ea, xrd, and tga showed that the chitin was in the alpha form. with respect to gender, two main differences were observed. first, we observed that the quantity of chitin was greater in males than in females an ... | 2015 | 25635814 |
| a small lytic polysaccharide monooxygenase from streptomyces griseus targeting α- and β-chitin. | the lytic polysaccharide monooxygenases (lpmos) have received considerable attention subsequent to their discovery because of their ability to boost the enzymatic conversion of recalcitrant polysaccharides. in the present study, we describe the enzymatic properties of sglpmo10f, a small (15 kda) auxilliary activity (aa) family 10 lpmo from streptomyces griseus belonging to a clade of the phylogenetic tree without any characterized representative. the protein was expressed using a brevibacillus-b ... | 2015 | 25605134 |
| stage of lactation and corresponding diets affect in situ protein degradation by dairy cows. | the influence of stage of lactation and corresponding diets on rates of protein degradation (kd) is largely unstudied. study objectives were to measure and compare in situ ruminal kd of crude protein (cp) and estimate rumen cp escape (rumen-undegradable protein; rup) of selected feeds by cows at 3 stages of lactation fed corresponding diets, and to determine the incubation times needed in an enzymatic in vitro procedure, using 0.2 units of streptomyces griseus protease per percent of true cp, th ... | 2014 | 25282425 |
| [the effect of enzymatic treatment using proteases on properties of persistent sodium current in ca1 pyramidal neurons of rat hippocampus]. | we investigated the effect of proteases, widely used for neuron isolation in electrophysiological studies, on the amplitude and kinetic characteristics of persistent sodium current (i(nap)) in hippocampal ca1 pyramidal neurons. properties of i(nap) were studied on neurons isolated by mechanical treatment (control group) and by mechanical and enzymatic treatment using pronase e (from streptomyces griseus) or protease type xxiii (from aspergillus oryzae). we show that in neurons isolated with pron ... | 2014 | 25097934 |
| a combined chemical and enzymatic method to determine quantitatively the polysaccharide components in the cell wall of yeasts. | a reliable method to determine cell wall polysaccharides composition in yeast is presented, which combines acid and enzymatic hydrolysis. sulphuric acid treatment is used to determine mannans, whereas specific hydrolytic enzymes are employed in a two sequential steps to quantify chitin and the proportion of β-(1,3) and β-(1,6)-glucan in the total β-glucan of the cell wall. in the first step, chitin and β-(1,3)-glucan were hydrolysed into their corresponding monomers n-acetylglucosamine and gluco ... | 2014 | 25041403 |
| anti-parasitic activities of specific bacterial extracellular products of streptomyces griseus sdx-4 against ichthyophthirius multifiliis. | the ciliate ichthyophthirius multifiliis is one of the most pathogenic parasites of fish maintained in captivity. in this study, effects of bacterial extracellular products of streptomyces griseus sdx-4 against i. multifiliis were determined. the fermentation liquor of s. griseus was extracted successively in a separating funnel with petroleum ether, ethyl acetate, and n-butanol. in vitro assays revealed that the n-butanol extracts (nbu-e) and ethyl acetate extracts (eto-e) of s. griseus were ob ... | 2014 | 25015048 |
| n-methylphenylalanyl-dehydrobutyrine diketopiperazine, an a-factor mimic that restores antibiotic biosynthesis and morphogenesis in streptomyces globisporus 1912-b2 and streptomyces griseus 1439. | the cell-free extracts of a landomycin e-producing strain, streptomyces globisporus 1912-2, were shown to contain a low-molecular-weight compound that, like a-factor, restored the landomycin e and streptomycin biosynthesis and sporulation of the defective mutants s. globisporus 1912-b2 and s. griseus 1439, respectively. the compound was purified by thin layer chromatography and hplc. it had an absorption maximum at λmax=245 nm and a molecular mass of m/z 244. on the basis of nmr spectroscopy ((1 ... | 2015 | 25005816 |
| retransformation of marker-free potato for enhanced resistance against fungal pathogens by pyramiding chitinase and wasabi defensin genes. | multi-auto-transformation vector system has been one of the strategies to produce marker-free transgenic plants without using selective chemicals and plant growth regulators and thus facilitating transgene stacking. in the study reported here, retransformation was carried out in marker-free transgenic potato cv. may queen containing chic gene (isolated from streptomyces griseus strain hut 6037) with wasabi defensin (wd) gene (isolated from wasabia japonica) to pyramid the two disease resistant g ... | 2014 | 24802621 |
| insights into the molecular interactions between aminopeptidase and amyloid beta peptide using molecular modeling techniques. | amyloid beta (aβ) peptides play a central role in the pathogenesis of alzheimer's disease. the accumulation of aβ peptides in ad brain was caused due to overproduction or insufficient clearance and defects in the proteolytic degradation of aβ peptides. hence, aβ peptide degradation could be a promising therapeutic approach in ad treatment. recent experimental report suggests that aminopeptidase from streptomyces griseus kk565 (sgak) can degrade aβ peptides but the interactive residues are yet to ... | 2014 | 24729013 |
| diversity of two-domain laccase-like multicopper oxidase genes in streptomyces spp.: identification of genes potentially involved in extracellular activities and lignocellulose degradation during composting of agricultural waste. | traditional three-domain fungal and bacterial laccases have been extensively studied for their significance in various biotechnological applications. growing molecular evidence points to a wide occurrence of more recently recognized two-domain laccase-like multicopper oxidase (lmco) genes in streptomyces spp. however, the current knowledge about their ecological role and distribution in natural or artificial ecosystems is insufficient. the aim of this study was to investigate the diversity and c ... | 2014 | 24657870 |
| antagonism of antifungal metabolites from streptomyces griseus h7602 against phytophthora capsici. | in this study, evidences for antagonism were established by production of antifungal metabolites from streptomyces griseus h7602, which were active to inhibit mycelial growth of phytophthora capsici in the in vitro assays. mycelial growth and zoosporangia formation of p. capsici was strongly inhibited in the medium containing the cell free culture filtrate of s. griseus h7602. antifungal metabolites from the cell free culture filtrate of s. griseus h7602 showed substantial antagonistic effects o ... | 2015 | 24554614 |
| host-parasite relations with a polyvalent streptomycophage from streptomyces griseus. | 1955 | 24543345 | |
| [intermediate products of glucides metabolism of streptomyces griseus]. | 2014 | 24542451 | |
| effect of cations upon multiplication of actinophage for streptomyces griseus. | 1951 | 24541660 | |
| production of soluble pigments by certain strains of streptomyces griseus. | 1951 | 24541659 | |
| rhodomycin--an antibiotic produced by a red-pigmented mutant of streptomyces griseus. | 1951 | 24540613 | |
| biosynthetic potential-based strain prioritization for natural product discovery: a showcase for diterpenoid-producing actinomycetes. | natural products remain the best sources of drugs and drug leads and serve as outstanding small-molecule probes to dissect fundamental biological processes. a great challenge for the natural product community is to discover novel natural products efficiently and cost effectively. here we report the development of a practical method to survey biosynthetic potential in microorganisms, thereby identifying the most promising strains and prioritizing them for natural product discovery. central to our ... | 2014 | 24484381 |
| improvement of catalytic efficiency and thermostability of recombinant streptomyces griseus trypsin by introducing artificial peptide. | streptomyces trypsin is one of the serine proteinases in streptomyces griseus and acts as a key mediator during cell growth and differentiation. s. griseus trypsin (sgt) could be successfully expressed in pichia pastoris by engineering the natural propeptide apnp. in this study, the recombinant exmt with peptide yvef and the wild-type sgt were comparatively investigated in detail. the recombinant exmt showed significantly increased thermostability which t(½) value was 3.89-fold of that of the sg ... | 2014 | 24458877 |
| two glycine riboswitches activate the glycine cleavage system essential for glycine detoxification in streptomyces griseus. | the glycine cleavage (gcv) system catalyzes the oxidative cleavage of glycine into co2, nh4(+), and a methylene group, which is accepted by tetrahydrofolate (thf) to form n(5),n(10)-methylene-thf. streptomyces griseus contains gcvp and the gcvt-gcvh operon, which encode three intrinsic components of the gcv system. we identified the transcriptional start sites of gcvth and gcvp and found putative glycine riboswitches in their 5' untranslated regions (5' utrs). the ratios of the transcripts of th ... | 2014 | 24443533 |
| characteristics of streptomyces griseus biofilms in continuous flow tubular reactors. | the purpose of this study was to investigate the feasibility of cultivating the biotechnologically important bacterium streptomyces griseus in single-species and mixed-species biofilms using a tubular biofilm reactor (tbr). streptomyces griseus biofilm development was found to be cyclical, starting with the initial adhesion and subsequent development of a visible biofilm after 24 h growth, followed by the complete detachment of the biofilm as a single mass, and ending with the re-colonisation of ... | 2014 | 24417230 |
| surface charge of protoplasts and their significance in cell-cell interaction. | ζ-potential of mesophyll protoplasts of tobacco (nicotiana tabacum l.), petunia (petunia hybrida hort.), turnip (brassica rapa l.) and cowpea (vigna unguiculata l. walpers) was determined by use of a cell electrophoresis apparatus. all protoplasts examined showed a constant negative value of-10 to-35 mv. the addition of cacl2 nullified the ζ-potential of tobacco protoplasts. this phenomenon is explained by dlvo theory of colloid science, which has been successfully applied to animal cells. furth ... | 1978 | 24408109 |
| [(2)h26]-1-epi-cubenol, a completely deuterated natural product from streptomyces griseus. | during growth on fully deuterated medium the volatile terpene [(2)h26]-1-epi-cubenol was released by the actinomycete streptomyces griseus. this compound represents the first completely deuterated terpene obtained by fermentation. despite a few previous reports in the literature the operability of this approach to fully deuterated compounds is still surprising, because the strong kinetic isotope effect of deuterium is known to slow down all metabolic processes in living organisms. potential appl ... | 2013 | 24367448 |
| hormonal control by a-factor of morphological development and secondary metabolism in streptomyces. | streptomyces griseus, a well-known industrial producer of streptomycin, is a member of the genus streptomyces, which shows a complex life cycle resembling that of fungi. a-factor, a c13 γ-butyrolactone compound, was discovered as a self-regulatory factor or a bacterial hormone to induce morphological differentiation and production of secondary metabolites, including streptomycin, in this organism. accumulating evidence has revealed an a-factor-triggered signal cascade, which is composed of sever ... | 2007 | 24367152 |
| regio- and stereospecific hydroxylation of various steroids at the 16α position of the d ring by the streptomyces griseus cytochrome p450 cyp154c3. | cytochrome p450 monooxygenases (p450s), which constitute a superfamily of heme-containing proteins, catalyze the direct oxidation of a variety of compounds in a regio- and stereospecific manner; therefore, they are promising catalysts for use in the oxyfunctionalization of chemicals. in the course of our comprehensive substrate screening for all 27 putative p450s encoded by the streptomyces griseus genome, we found that escherichia coli cells producing an s. griseus p450 (cyp154c3), which was fu ... | 2014 | 24334658 |
| activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster. | polycyclic tetramate macrolactams (ptms) are a widely distributed class of natural products with important biological activities. however, many of these ptms have not been characterized. here we apply a plug-and-play synthetic biology strategy to activate a cryptic ptm biosynthetic gene cluster sgr810-815 from streptomyces griseus and discover three new ptms. this gene cluster is highly conserved in phylogenetically diverse bacterial strains and contains an unusual hybrid polyketide synthase-non ... | 2013 | 24305602 |
| genome mining of streptomyces ambofaciens. | since the discovery of the streptomycin produced by streptomyces griseus in the middle of the last century, members of this bacterial genus have been largely exploited for the production of secondary metabolites with wide uses in medicine and in agriculture. they have even been recognized as one of the most prolific producers of natural products among microorganisms. with the onset of the genomic era, it became evident that these microorganisms still represent a major source for the discovery of ... | 2014 | 24258629 |
| suppression of the powdery mildew pathogen by chitinase microinjected into barley coleoptile epidermal cells. | an exogenous chitinase from streptomyces griseus was introduced into coleoptile epidermal cells of barley (hordeum vulgare) by microinjection, and the effect of injected chitinase on the growth or development of the powdery mildew pathogen (erysiphe graminis f. sp. hordei) was examined. prior to microinjection, an enzymatic degradation of fungal haustorium, the organ taking nutrients from host plant cells, was examined by treating fixed coleoptile epidermis harboring haustoria with this enzyme. ... | 1991 | 24221582 |
| transformation of ferulic acid to vanillin using a fed-batch solid-liquid two-phase partitioning bioreactor. | amycolatopsis sp. atcc 39116 (formerly streptomyces setonii) has shown promising results in converting ferulic acid (trans-4-hydroxy-3-methoxycinnamic acid; substrate), which can be derived from natural plant wastes, to vanillin (4-hydroxy-3-methoxybenzaldehyde). after exploring the influence of adding vanillin at different times during the growth cycle on cell growth and transformation performance of this strain and demonstrating the inhibitory effect of vanillin, a solid-liquid two-phase parti ... | 2014 | 24167066 |
| specificity of proteinase k at p2 to p3' sub-sites and its comparison to other serine proteases. | specificity of the commercially important serine protease, proteinase k, has been investigated by measuring free energies of association of proteinase k with turkey ovomucoid third domain inhibitor variants at contact positions p2, p1, p1', p2', and p3'. correlations of these values were run with similar values that have been obtained for six other serine proteases. among the six proteases, subtilisin carlsberg shows a near perfect correlation (pearson product correlation coefficient = 0.93 to 0 ... | 2014 | 24050203 |
| complex structure of the dna-binding domain of adpa, the global transcription factor in streptomyces griseus, and a target duplex dna reveals the structural basis of its tolerant dna sequence specificity. | adpa serves as the global transcription factor in the a-factor regulatory cascade, controlling the secondary metabolism and morphological differentiation of the filamentous bacterium streptomyces griseus. adpa binds to over 500 operator regions with the consensus sequence 5'-tggcsngwwy-3' (where s is g or c, w is a or t, y is t or c, and n is any nucleotide). however, it is still obscure how adpa can control hundreds of genes. to elucidate the structural basis of this tolerant dna recognition by ... | 2013 | 24019524 |
| biotransformation of dihydroisosteviol and the effects of transformed products on steroidogenic gene expressions. | the biotransformation of dihydroisosteviol with absidia pseudocylindrospora atcc 24169, streptomyces griseus atcc 10137, mucor recurvatus mr36, and aspergillus niger bcrc 31130 yielded 15 metabolites, eight of which were previously unknown. structures of metabolites were established by 2d nmr techniques and hrms data, two of which were further corroborated by chemical means, and another via single-crystal x-ray diffraction analysis. subsequently, two steroidogenic cell lines (y-1 mouse adrenal t ... | 2013 | 23948258 |
| [heterologous expression and enzymatic analysis of streptomyces griseus trypsin in streptomyces lividans]. | trypsin as an important serine protease has been widely used in food, pharmaceutical and tanning industries. in this study, we successfully expressed trypsin (cloning from streptomyces griseus atcc10137) in streptomyces lividans tk24 and comparatively investigated its enzymatic properties. specifically, applying s. griseus atcc 10137 genome as template, we obtained the sprt gene and sub-cloned it into the expression plasmid pij86, generating the recombinant strain s. lividans tk24/pij86-sprt. wh ... | 2013 | 23894820 |
| additivity-based design of the strongest possible turkey ovomucoid third domain inhibitors for porcine pancreatic elastase (ppe) and streptomyces griseus protease b (sgpb). | we describe here successful designs of strong inhibitors for porcine pancreatic elastase (ppe) and streptomyces griseus protease b (sgpb). for each enzyme two inhibitor variants were designed. in one, the reactive site residue (position 18) was retained and the best residues were substituted at contact positions 13, 14, and 15. in the other variant the best residues were substituted at all contact positions except the reactive site where a gly was substituted. the four designed variants were: fo ... | 2013 | 23892073 |
| microbial biotransformation of aryl sulfanylpentafluorides. | we report, for the first time, the biotransformation of potential pollutants bearing the pentafluorosulfanyl (sf5-) functional group in a fungus and bacteria. cunninghamella elegans transformed p-methoxy phenyl sf5 via demethylation; pseudomonas knackmussii and p. pseudoalcaligenes kf707 transformed amino-, hydroxyamino- and diamino- substituted phenyl sf5, forming the n-acetylated derivatives as the main product. cell-free extract of streptomyces griseus transformed 4-amino-3-hydroxy-phenyl sf5 ... | 2014 | 23872898 |
| predictive model for epoxide hydrolase-generated stereochemistry in the biosynthesis of nine-membered enediyne antitumor antibiotics. | nine-membered enediyne antitumor antibiotics c-1027, neocarzinostatin (ncs), and kedarcidin (ked) possess enediyne cores to which activity-modulating peripheral moieties are attached via (r)- or (s)-vicinal diols. we have previously shown that this stereochemical difference arises from hydrolysis of epoxide precursors by epoxide hydrolases (ehs) with different regioselectivities. the inverting ehs, such as sgcf, hydrolyze an (s)-epoxide substrate to yield an (r)-diol in c-1027 biosynthesis, wher ... | 2013 | 23844627 |
| chitinase activity on amorphous chitin thin films: a quartz crystal microbalance with dissipation monitoring and atomic force microscopy study. | chitinases are widely distributed in nature and have wide-ranging pharmaceutical and biotechnological applications. this work highlights a real-time and label-free method to assay chitinase activity via a quartz crystal microbalance with dissipation monitoring (qcm-d) and atomic force microscopy (afm). the chitin substrate was prepared by spincoating a trimethylsilyl chitin solution onto a silica substrate, followed by regeneration to amorphous chitin (rchi). the qcm-d and afm results clearly sh ... | 2013 | 23822524 |
| novel marine actinobacteria from emerald andaman & nicobar islands: a prospective source for industrial and pharmaceutical byproducts. | andaman and nicobar islands situated in the eastern part of bay of bengal are one of the distinguished biodiversity hotspot. even though number of studies carried out on the marine flora and fauna, the studies on actinobacteria from andaman and nicobar islands are meager. the aim of the present study was to screen the actinobacteria for their characterization and identify the potential sources for industrial and pharmaceutical byproducts. | 2013 | 23800234 |
| biological pretreatment of lignocellulosic substrates for enhanced delignification and enzymatic digestibility. | sheer enormity of lignocellulosics makes them potential feedstock for biofuel production but, their conversion into fermentable sugars is a major hurdle. they have to be pretreated physically, chemically, or biologically to be used by fermenting organisms for production of ethanol. each lignocellulosic substrate is a complex mix of cellulose, hemicellulose and lignin, bound in a matrix. while cellulose and hemicellulose yield fermentable sugars, lignin is the most recalcitrant polymer, consistin ... | 2011 | 23729871 |
| organization and characterization of a biosynthetic gene cluster for bafilomycin from streptomyces griseus dsm 2608. | streptomyces griseus dsm 2608 produces bafilomycin, an antifungal plecomacrolide antibiotic. we cloned and sequenced an 87.4-kb region, including a polyketide synthase (pks) region, methoxymalonate genes, flavensomycinate genes, and other putative regulatory genes. the 58.5kb of pks region consisting 12 pks modules arranged in five different pks genes, was assumed to be responsible for the biosynthesis of plecomacrolide backbone including 16-membered macrocyclic lactone. all the modules showed h ... | 2013 | 23663353 |
| rational design of a novel propeptide for improving active production of streptomyces griseus trypsin in pichia pastoris. | applying in silico simulations and in vitro experiments, the amino acid proline was proved to have a profound influence on streptomyces griseus trypsinogen, and the hydrogen bond between h(57) and d(102) was found to be crucial for trypsin activity. by introducing an artificial propeptide, ivef, the titer of trypsin was increased 6.71-fold. | 2013 | 23563937 |
| human cervicofacial mycetoma caused by streptomyces griseus: first case report. | streptomyces griseus causes subcutaneous mycetomas in felines and dolphins; however, human mycetoma caused by s. griseus has not previously been reported. hereby, we report a case of a 50-year-old female presenting with swelling in the left upper cervical region and the left cheek that lasted for 6 months. the fine needle aspiration (fna) performed on the swelling yielded purulent material; on microscopy, actinomycosis was diagnosed. on culturing, the pus grew s. griseus. to the best of our know ... | 2015 | 23465713 |
| an alternative sigma factor governs the principal sigma factor in streptomyces griseus. | in bacteria, the rna polymerase holoenzyme comprises a five-subunit core enzyme and a dissociable subunit, sigma factor, which is responsible for transcriptional initiation. the filamentous bacterium streptomyces griseus has 52 sigma factors, including one essential 'principal' sigma factor (σ(hrdb) ) that is responsible for the transcription of housekeeping genes. here we characterized an alternative sigma factor (σ(shba) ), which is highly conserved within the genus streptomyces. a σ(shba) -de ... | 2013 | 23347076 |
| a convenient chemical-microbial method for developing fluorinated pharmaceuticals. | a significant proportion of pharmaceuticals are fluorinated and selecting the site of fluorine incorporation can be an important beneficial part a drug development process. here we describe initial experiments aimed at the development of a general method of selecting optimum sites on pro-drug molecules for fluorination, so that metabolic stability may be improved. several model biphenyl derivatives were transformed by the fungus cunninghamella elegans and the bacterium streptomyces griseus, both ... | 2013 | 23299916 |
| divergent effects of desferrioxamine on bacterial growth and characteristics. | desferrioxamines (df's) are siderophores produced by some groups of bacteria. previously, we discovered that dfe, produced by streptomyces griseus, induced divergent developmental phenotypes in various streptomyces isolates. in this study, we isolated bacteria whose phenotype was affected by the presence of 0.1 mm dfb from soil samples, and studied their phylogenetic position via 16 s rrna gene-based analysis. isolates belonging to microbacterium grew only in the presence of dfb in the medium. d ... | 2013 | 23232933 |
| microbial transformation of asiatic acid. | asiatic acid (1), a major pentacyclic triterpene of centella asiatica, was subjected to transformation by penicillium lilacinum accc 31890, fusarium equiseti cgmcc 3.3658, and streptomyces griseus cgmcc 4.18 strains. incubation of asiatic acid with p. lilacinum accc 31890 and f. equiseti cgmcc 3.3658 gave an identical product: 2α,3β,15α,23-tetrahydroxyurs-12-en-28-oic acid (2). biotransformation of asiatic acid by s. griseus cgmcc 4.18 resulted in three derivatives: 2α,3β,21β,23-tetrahydroxyurs- ... | 2013 | 23227815 |
| proteomic analysis of the streptomyces griseus ribosomal fraction. | the streptomyces griseus 70s ribosome fraction was analyzed by radical-free and highly reducing two-dimensional (rfhr 2d) gel electrophoresis and mass spectrometry. among the 60 putative ribosomal proteins that are encoded by the s. griseus genome, 48 were identified in the 70s ribosome fraction prepared from mycelia grown in liquid culture for 12, 36, and 48 h. ribosomal protein s3 was detected at two different positions on the 2d gel, and the distribution changed completely in the course of th ... | 2012 | 23221706 |
| chemical and microbial semi-synthesis of tetrahydroprotoberberines as inhibitors on tissue factor procoagulant activity. | to discover new inhibitors on tissue factor procoagulant activity, 21 tetrahydroprotoberberines were screened on the model of human thp-1 cells stimulated by lipopolysaccharide. among these tetrahydroprotoberberines, several unique compounds were synthesized through microbial transformation: compound 6 (l-corydalmine) was obtained through regio-selective demethylation by streptomyces griseus atcc 13273, whereas compounds 4a, 4b, 5h, and 5i were microbial glycosylation products by gliocladium del ... | 2013 | 23199480 |
| purification and structure elucidation of the by-product of new regulator of antibiotic production and differentiation of streptomyces. | streptomyces globisporus 1912, a producer of the antitumor antibiotic landomycin e, forms the new low-molecular signaling molecule n-methylphenylalanyl-dehydrobutyrine diketopiperazine (bdd) and its complex and unstable by-product which restore, like the a-factor in streptomyces griseus 773, landomycin e and streptomycin biosynthesis, and sporulation of the defective mutants s. globisporus 1912-b2 and s. griseus 1439, respectively. here, we report the purification and structure elucidation of tw ... | 2012 | 23120988 |
| protease-catalysed direct asymmetric mannich reaction in organic solvent. | we reported the first enzyme-catalysed, direct, three-component asymmetric mannich reaction using protease type xiv from streptomyces griseus (sgp) in acetonitrile. yields of up to 92% with enantioselectivities of up to 88% e.e. and diastereoselectivities of up to 92:8 (syn:anti) were achieved under the optimised conditions. this enzyme's catalytic promiscuity expands the application of this biocatalyst and provides a potential alternative method for asymmetric mannich reactions. | 2012 | 23094136 |
| identification of the sgr6065 gene product as a sesquiterpene cyclase involved in (+)-epicubenol biosynthesis in streptomyces griseus. | recent bacterial genome sequencing projects have shown the presence of many putative sesquiterpene cyclase (sc) genes, especially in the gram-positive, filamentous bacterial genus streptomyces. we describe here the characterization of a sc gene (sgr6065, named geca) from streptomyces griseus. overexpression of geca in streptomyces lividans produced a sesquiterpene, which was isolated and determined to be (+)-epicubenol using spectroscopic analyses. the n-terminal histidine-tagged geca protein wa ... | 2012 | 22872183 |
| purification, crystallization and preliminary x-ray analysis of the dna-binding domain of adpa, the central transcription factor in the a-factor regulatory cascade in the filamentous bacterium streptomyces griseus, in complex with a duplex dna. | streptomyces griseus adpa is the central transcription factor in the a-factor regulatory cascade and activates a number of genes that are required for both secondary metabolism and morphological differentiation, leading to the onset of streptomycin biosynthesis as well as aerial mycelium formation and sporulation. the dna-binding domain of adpa consists of two helix-turn-helix dna-binding motifs and shows low nucleotide-sequence specificity. to reveal the molecular basis of the low nucleotide-se ... | 2012 | 22869129 |
| a furantaxane with an unusual 6/8/6/5 ring system and potent tumor mdr reversal activity obtained via microbial transformation. | a furantaxane (4) with an unusual 6/8/6/5 ring system and two hydroxylated products (2, 3) were isolated following the biotransformation of a taxane (1) by streptomyces griseus. the structures of the isolates were elucidated by spectroscopic analysis. the absolute configuration of 4, which exhibited potent reversal activity in the a549/taxol mdr tumor cell line, was unambiguously deduced by single-crystal x-ray diffraction. | 2012 | 22852759 |
| functional expression of trypsin from streptomyces griseus by pichia pastoris. | in the present study, the genes encoding trypsinogen and active trypsin from streptomyces griseus were both cloned and expressed in the methylotrophic yeast pichia pastoris with the α-factor secretion signal under the control of the alcohol oxidase promoter. the mature trypsin was successfully accumulated extracellularly in soluble form with a maximum amidase activity of 6.6 u ml(-1) (batch cultivation with flask cultivation) or 14.4 u ml(-1) (fed-batch cultivation with a 3-l fermentor). in cont ... | 2012 | 22842958 |
| lethal effect of streptomyces citreofluorescens against larvae of malaria, filaria and dengue vectors. | to investigate lethal effect of culture filtrates of streptomyces citreofluorescens (s. citreofluorescens) against anopheles stephensi (an. stephensi), culex quinquefasciatus (cx. quinquefasciatus), and aedes aegypti (ae. aegypti) larvae vectors for malaria, filarial and dengue. | 2012 | 22840445 |
| nutrient digestibility and evaluation of protein and carbohydrate fractionation of citrus by-products. | the protein and carbohydrate fractionation and nutrient digestibility of citrus by-products were determined. ruminal, intestinal and total tract cp disappearance values were measured by a modified three-step (mtsp) method and in vitro cp disappearance method (ivcp). test feeds were orange pulp (op), lime pulp (lp), lemon pulp (lep), grapefruit pulp (gp), sweet lemon pulp (slp), bitter lemon pulp (blp), bergamot orange pulp (bp) and tangerine pulp (tp). the rumen undegradable protein (rup) fracti ... | 2013 | 22703299 |
| functional modulation of a protein folding landscape via side-chain distortion. | ultrahigh-resolution (< 1.0 å) structures have revealed unprecedented and unexpected details of molecular geometry, such as the deformation of aromatic rings from planarity. however, the functional utility of such energetically costly strain is unknown. the 0.83 å structure of α-lytic protease (αlp) indicated that residues surrounding a conserved phe side-chain dictate a rotamer which results in a ~6° distortion along the side-chain, estimated to cost 4 kcal/mol. by contrast, in the closely rela ... | 2012 | 22635267 |
| characterization of new class iii lantibiotics--erythreapeptin, avermipeptin and griseopeptin from saccharopolyspora erythraea, streptomyces avermitilis and streptomyces griseus demonstrates stepwise n-terminal leader processing. | lantibiotics are a large group of ribosomally synthesized peptides post-translationally modified to incorporate the amino acid lanthionine. they are classified, according to their biosynthetic pathway and bioactivity, into three major subtypes. of actinomycetes type iii lantibiotics, only four peptides (sapb, sapt, laba1, and laba2) have been described and structurally characterized, although homologous gene clusters are abundant in other actinomycetes. all these gene clusters share a similar ar ... | 2012 | 22556031 |
| purification of an antifungal endochitinase from a potential biocontrol agent streptomyces griseus. | streptomyces griseus (mtcc 9723) is a chitinolytic bacterium isolated from prawn cultivated pond soil of peddapuram village; east godavari district was studied in detailed. chitinase (ec 3.2.1.14) was extracted from the culture filtrate of streptomyces griseus and purified by ammonium sulfate precipitation, deae-cellulose ionexchange chromatography, sephadex g-100 and sephadex g-200 gel filtration chromatography. the molecular mass of the purified chitinase was estimated to be 34, 32 kda by sds ... | 2011 | 22545353 |
| diversity analysis of streptomycetes and associated phosphotranspherase genes in soil. | an attempt was made to verify the observation that streptomyces griseus was prevalent in soil based on isolation work. a genus-specific pcr was developed for streptomyces based on the housekeeping gene atpd and used to investigate species diversity within selected soils. the presence of s. griseus was investigated to determine coexistence of resistance-only streptomycin phosphotransferase (stra) in the same soil as streptomycin producers. two additional pcr-based assays were developed; one speci ... | 2012 | 22540003 |
| taxonomic identification of a novel strain of streptomyces cavourensis subsp. washingtonensis, acma006, exhibiting antitumor and antibacteria activity. | taxonomically diverse and genetically specialized, marine microorganisms have great potential in generating bioactive substances. a previous study isolated a novel actinomycete strain designated acma006 and revealed that the fermentation broth of acma006 (fba6) significantly inhibited the growth of a series of tumor cell lines. the present study examined various characteristics of the acma006 strain, including its morphological, physiological, and biochemical nature, and the 16s rdna gene sequen ... | 2010 | 22491305 |
| genome-wide distribution of adpa, a global regulator for secondary metabolism and morphological differentiation in streptomyces, revealed the extent and complexity of the adpa regulatory network. | adpa is a global transcriptional activator triggering morphological differentiation and secondary metabolism in streptomyces griseus. adpa influences the expression of >1000 genes; however, the overall picture of the adpa regulon remains obscure. here, we took snapshots of the distribution of adpa across the chromosome in living s. griseus cells using chromatin immunoprecipitation/chromatin affinity precipitation-seq analysis. in both liquid and solid cultures, adpa bound to >1200 similar sites, ... | 2012 | 22449632 |
| characterization and antimicrobial activities of two streptomyces isolates from soil in the periphery of universiti putra malaysia. | this study was to assess the identification and antimicrobial activities of two actinomycete isolates. the two isolates designated as b8 and c2, were isolated from a patch of soil in the peripheral area of universiti putra malaysia by streaking on starch casein agar after standard serial dilution procedures. their antimicrobial activities were first evaluated against eight clinical laboratory strains namely bacillus sp., enterococcus sp., escherichia coli, klebsiella sp., pseudomonas sp., salmon ... | 2011 | 22433896 |
| structural changes in freshwater fish and chironomids exposed to bacterial exotoxins. | mass fish mortalities have been reported in the past decade from british waters, often coinciding with blooms of filamentous actinobacteria, particularly strains of streptomyces griseus. the present study has shown that some fractions of the exudate of s. griseus, prepared after a series of high pressure liquid chromatography (hplc) separations, and analysed with liquid chromatography mass spectrometry (lc-ms), induced pathological changes to the gills of carp and/or tench fry following exposure ... | 2012 | 22381615 |
| reassessment of the status of streptomyces setonii and reclassification of streptomyces fimicarius as a later synonym of streptomyces setonii and streptomyces albovinaceus as a later synonym of streptomyces globisporus based on combined 16s rrna/gyrb gene sequence analysis. | the 16s rrna and gyrb genes of 22 streptomyces strains belonging to the streptomyces griseus cluster were sequenced, and their taxonomic positions were re-evaluated. for correct analysis, all of the publicly available sequences of the species were collected and compared with those obtained in this study. species for which no consensus sequence could be identified were excluded from the phylogenetic analysis. the levels of 16s rrna gene sequence similarity within the cluster ranged from 98.6 to 1 ... | 2012 | 22286909 |
| the o-methyltransferase srsb catalyzes the decarboxylative methylation of alkylresorcylic acid during phenolic lipid biosynthesis by streptomyces griseus. | streptomyces griseus contains the srs operon, which is required for phenolic lipid biosynthesis. the operon consists of srsa, srsb, and srsc, which encode a type iii polyketide synthase, an o-methyltransferase, and a flavoprotein hydroxylase, respectively. we previously reported that the recombinant srsa protein synthesized 3-(13'-methyltetradecyl)-4-methylresorcinol, using iso-c(16) fatty acyl-coenzyme a (coa) as a starter substrate and malonyl-coa and methylmalonyl-coa as extender substrates. ... | 2012 | 22247507 |