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oral microbial profiles of individuals with different levels of sugar intake.the aim was to compare the oral microbial profiles in young adults with an intake of free sugars above or below the current recommendations by the who for sugar consumption. seventy subjects completed a quantitative food frequency questionnaire to establish the proportion of free sugars in relation to the total energy intake (% e). subjects with <5% e (n = 30) formed the low-sugar group, while those with ≥5% e (n = 40) were regarded as reference group. saliva and plaque samples were analyzed by ...201728839520
characterization and antimicrobial efficacy of portland cement impregnated with silver nanoparticles.this study investigated the effects of silver nanoparticle (sn) loading into hydraulic calcium silicate-based portland cement on its mechanical, antibacterial behavior and biocompatibility as a novel dental bone substitute.201728680554
salivary density of streptococcus mutans and streptococcus sobrinus and dental caries in children and adolescents with down syndrome.to assess and compare dental caries experience and salivary s. mutans, s. sobrinus, and streptococci counts between groups of down syndrome and non-down syndrome children and adolescents.201728678943
the oral bacterial microbiome of occlusal surfaces in children and its association with diet and caries.dental caries is the most prevalent disease in humans globally. efforts to control it have been invigorated by an increasing knowledge of the oral microbiome composition. this study aimed to evaluate the bacterial diversity in occlusal biofilms and its relationship with clinical surface diagnosis and dietary habits. anamneses were recorded from thirteen 12-year-old children. biofilm samples collected from occlusal surfaces of 46 permanent second molars were analyzed by 16s rrna amplicon sequenci ...201728678838
effect of arginine on the growth and biofilm formation of oral bacteria.alkali production via arginine deiminase system (ads) of oral bacteria plays a significant role in oral ecology, ph homeostasis and inhibition of dental caries. ads activity in dental plaque varies greatly between individuals, which may profoundly affect their susceptibility to caries.201728668766
antimicrobial and biological activity of leachate from light curable pulp capping materials.characterization of a number of pulp capping materials and assessment of the leachate for elemental composition, antimicrobial activity and cell proliferation and expression.201728645637
improved method for rapid and accurate isolation and identification of streptococcus mutans and streptococcus sobrinus from human plaque samples.mutans streptococci (ms), specifically streptococcus mutans (sm) and streptococcus sobrinus (ss), are bacterial species frequently targeted for investigation due to their role in the etiology of dental caries. differentiation of s. mutans and s. sobrinus is an essential part of exploring the role of these organisms in disease progression and the impact of the presence of either/both on a subject's caries experience. of vital importance to the study of these organisms is an identification protoco ...201728606792
in-vitro efficacy of different morphology zinc oxide nanopowders on streptococcus sobrinus and streptococcus mutans.zno with two different morphologies were used to study the inhibition of streptococcus sobrinus and streptococcus mutans which are closely associated with tooth cavity. rod-like shaped zno-a and plate-like shaped zno-b were produced using a zinc boiling furnace. the nanopowders were characterized using energy filtered transmission electron microscopy (eftem), x-ray diffraction (xrd), photoluminescence (pl) spectroscopy, raman spectroscopy and dynamic light scattering (dls) to confirm the propert ...201728576061
chemical composition, antibacterial, schistosomicidal, and cytotoxic activities of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae).we have investigated the chemical composition and the antibacterial activity of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae) (da-eo) against a representative panel of cariogenic bacteria. we have also assessed the in vitro schistosomicidal effects of da-eo on schistosoma mansoni and its cytotoxicity to gm07492-a cells in vitro. gas chromatography (gc) and gas chromatography-mass spectrometry (gc/ms) revealed that the monoterpenes cis-piperitone oxide (35. ...201728504841
compositional differences in multi-species biofilms formed on various orthodontic adhesives.to investigate the aspects of multi-species biofilm formation on various orthodontic adhesives with different surface characteristics.201728339597
[influence of surface roughness on oral streptococcal adhesion forces to dental filling materials].this study is to determine the common oral streptococcal adhesion forces by using composite resin and glass ionomer cement (gic) with different degrees of surface roughness via atomic force microscopy (afm) analysis. the influence of surface roughness on bacterial adhesion force is also discussed.201628326699
evaluation of the antibacterial effects of aqueous and ethanolic leaf extracts of aloysia citriodora (lemon verbena) on streptococcus mutans and streptococcus sobrinus.the aloysia citriodora plant from the family of verbenaceae has many uses in traditional medicine. the aim of the current study was to determine the effects of the aqueous and ethanolic extracts of a. citriodora on streptococcus mutans and streptococcus sobrinus, which cause tooth decay.201628163849
distribution of streptococcus mutans and streptococcus sobrinus in dental plaque of indian pre-school children using pcr and sb-20m agar medium.dental caries is one of the most common infectious diseases affecting the oral cavity. among the oral bacteria, mutans streptococci have been implicated as major cariogenic bacteria as they can produce high levels of dental caries causing substances such as lactic acid and extracellular polysaccharides.201628050506
five-year longitudinal study of dental caries risk associated with streptococcus mutans and streptococcus sobrinus in individuals with intellectual disabilities.streptococcus mutans (s. mutans) and streptococcus sobrinus (s. sobrinus) are important etiologic agents in human dental caries. using quantitative real-time polymerase chain reaction assays for the presence of those strains, we examined 145 outpatients with intellectual disability (id), calculated the proportion of each of these strains to total bacteria, and compared dental caries incidence over 5 years. plaque samples were collected from all erupted tooth sites, and dental examinations were p ...201728049966
comparative study on the characteristics of weissella cibaria cmu and probiotic strains for oral care.probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. the present work was conducted to compare the characteristics of oral care probiotics: weissella cibaria cmu (chonnam medical university) and four commercial probiotic strains. survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and i ...201627999400
no evidence for the growth-stimulating effect of monomers on cariogenic streptococci.in spite of contradicting results, the high susceptibility of composites for secondary caries is still often associated with the bacterial growth-stimulating effect of released methacrylate monomers. however, most studies that showed this effect were performed with techniques having inherent limitations (spectrophotometry).201627766489
the effect of seasons on brazilian red propolis and its botanical source: chemical composition and antibacterial activity.the aim of this study was to evaluate the effect of seasons on the chemical composition and antibacterial activity of brazilian red propolis (brp) and its plant source. brp was collected from maceio, alagoas state, north-east of brazil, during one year. chemical composition was determined by physicochemical analyses and hplc while antimicrobial activity was assessed against streptococcus mutans, streptococcus sobrinus, staphylococcus aureus and actinomyces naeslundii by determining the minimal i ...201727701899
comparative in vitro investigation of the cariogenic potential of bifidobacteria.this study aimed to assess the in vitro cariogenic potential of some bifidobacterium species in comparison with caries-associated bacteria.201627475723
synthesis and screening of n-acyl thiolated chitosans for antibacterial applications.low-molecular weight chitosan-thioglycolic acid has shown significant antibacterial properties against different microorganisms. in order to explore the potential and structure-activity relationships of newly synthesized alkyl thiomers, chitosan has been functionalized with a series of thio-acids with increasing alkyl chain length. all thiomers were characterized with special emphasis on the determination of their degree of deacetylation and substitution, as well as on their molecular weight and ...201627474669
inhibition of glucan synthesis by casein polymers crosslinked by glutaraldehyde.glutaraldehyde-crosslinked α-casein polymers with molecular masses of 1-5×10(5) were found to inhibit adhesive insoluble glucan formation catalyzed by glucan synthases from streptococcus sobrinus b13, a cariogenic oral bacterium. of the three subcomponents of the glucan synthases tested, primer-dependent insoluble glucan synthase was inhibited specifically by the m r~2.7×10(5) polymer at a concentration of 1.4-8.5 μg/ml. the polymer was also active in inhibiting the artificial plaque formation b ...199827393366
streptococcusdentiloxodontae sp. nov., isolated from the oral cavity of elephants.a gram-stain-positive, catalase-negative, coccus-shaped organism was isolated from oral cavity samples collected from healthy elephants. the isolated strain, num 2404t, was tentatively identified as a streptococcal species based on the results of biochemical tests. although a comparative 16s rrna gene sequence analysis suggested the classification of this organism into the genus streptococcus, it did not correspond to any recognized species of the genus. strain num 2404t was related most closely ...201627381035
microbial ecosystem analysis in root canal infections refractory to endodontic treatment.the purpose of this study was to combine multiple displacement amplification and checkerboard dna-dna hybridization to qualitatively and quantitatively evaluate the microbiota present in infections refractory to endodontic treatment.201627377440
streptococcus mutans, streptococcus sobrinus and candida albicans in oral samples from caries-free and caries-active children.this was to examine the occurrence of s. mutans, s. sobrinus and c. albicans in dental plaque and saliva from caries-free and caries-active greek children.201627357362
pleurocidin congeners demonstrate activity against streptococcus and low toxicity on gingival fibroblasts.fish epidermal antimicrobial peptides, such as pleurocidin, are cathelicidins with broad-spectrum antimicrobial activity against gram negative and gram-positive bacteria, as well as fungi. in the current study, we attempted to optimize peptide bioactivity by sequence modification and assess the antimicrobial activities.201627341459
influence of sucrose and xylitol on an early streptococcus mutans biofilm in a dental simulator.in vitro methods to study dental biofilms are useful in finding ways to support a healthy microbial balance in the oral cavity. the effects of sucrose, xylitol, and their combination on three strains of streptococcus mutans and one strain of streptococcus sobrinus were studied using a dental simulator.201627318453
differential utilization of basic proline-rich glycoproteins during growth of oral bacteria in saliva.although saliva is widely recognized as a primary source of carbon and nitrogen for growth of the dental plaque biofilm community, little is known about how different oral bacteria utilize specific salivary components. to address this question, 32 strains representing 16 genera commonly isolated from early plaque biofilms were compared for growth over two transfers in stimulated (by chewing parafilm) whole saliva that was stabilized by heat treatment and dialysis. the cell densities, measured by ...201627316966
evaluation of antibacterial efficiency of chitosan and chitosan nanoparticles on cariogenic streptococci: an in vitro study.the most prevalent and worldwide oral disease is dental caries that affects a significant proportion of the world population. there are some classical approaches for control, prevention and treatment of this pathologic condition; however, the results are still not completely successful. therefore new methods are needed for better management of this important challenge. chitosan is a natural and non-toxic polysaccharide with many biological applications, particularly as an antimicrobial agent. ch ...201627307974
inhibitory effects of ellagic acid on glucosyltransferases from mutans streptococci.ellagic acid and its derivatives were found to abrogate the enzymatic activity of the glucosyltransferases (gtases) of mutans streptococci. these compounds preferentially inhibited the water-soluble glucan synthesis by gtase of streptococcus mutans mt8148 and inhibited the water-insoluble glucan synthesis by gtase of streptococcus sobrinus 6715. this inhibition resulted in a reduction of cellular adherence of mutans streptococci to a glass surface.199227286205
galla chinensis compounds remineralize enamel caries lesions in a rat model.to investigate the effect of galla chinensis chemical compounds on enamel caries remineralization in rats.201627111668
frequency of dental caries in active and inactive systemic lupus erythematous patients: salivary and bacterial factors.the objective of this study was to determine dental caries frequency and to analyze salivary and bacterial factors associated with active and inactive systemic lupus erythematous (sle) patients. also, a proposal to identify dental caries by a surface, teeth, and the patient was developed.201627053402
the comparative evaluation of the antimicrobial effect of propolis with chlorhexidine against oral pathogens: an in vitro study.this study aimed to compare the antimicrobial effectiveness of ethanolic extract of propolis (eep) to chlorhexidine gluconate (chx) on planktonic streptococcus mutans, streptococcus sobrinus, lactobacillus acidophilus, lactobacillus salivarius subsp. salivarius, aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, staphylococcus aureus, enterococcus faecalis, actinomyces israelii, candida albicans, and their single-species biofilms by agar dilution and broth mi ...201626949701
pcr-dipstick dna chromatography for profiling of a subgroup of caries-associated bacterial species in plaque from healthy coronal surfaces and periodontal pockets.the onset of plaque-mediated disease, including dental caries and periodontal diseases, is highly associated with compositional change of the resident microflora from the ecological perspective. as specific bacterial profiles have been linked to different disease stages, microbial compositional measurements might therefore have great value for clinical diagnosis. previously we have reported a dry-reagent strip biosensor-pcr-dipstick dna chromatography, which utilized molecular recognition of oli ...201626912138
antibacterial effect of hydroalcoholic extract of punica granatum linn. petal on common oral microorganisms.objectives. this study aimed to assess the effect of hydroalcoholic extract of punica granatum linn. (p. granatum) petal on streptococcus sanguinis, streptococcus mutans, streptococcus salivarius, streptococcus sobrinus, and enterococcus faecalis. materials and methods. in this in vitro study, p. granatum extract was prepared using powdered petals and water-ethanol solvent. antibacterial effect of the extract, chlorhexidine (chx), and ampicillin was evaluated on brain heart infusion agar (bhia) ...201626884763
antibacterial peptides: opportunities for the prevention and treatment of dental caries.dental caries is a multifactorial disease that is a growing and costly global health concern. the onset of disease is a consequence of an ecological imbalance within the dental plaque biofilm that favors specific acidogenic and aciduric caries pathogens, namely streptococcus mutans and streptococcus sobrinus. it is now recognized by the scientific and medical community that it is neither possible nor desirable to totally eliminate dental plaque. conversely, the chemical biocides most commonly us ...201126781572
effect of milk and yogurt on streptococcus sobrinus counts and caries score in rats.an anti-cariogenic diet containing probiotics can be effective in caries prevention. this animal study compared the effects of milk and yogurt on streptococcus sobrinus counts and caries score.201726759594
[effects of yili dark bee propolis on oral cariogenic biofilm in vitro].to evaluate the effects of yili dark bee propolis on the main cariogenic biofilm and mechanisms.201526595940
randomized in vivo evaluation of photodynamic antimicrobial chemotherapy on deciduous carious dentin.the aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control [chlorhexidine and resin-modified glass ionomer cement (rmgic)], ledtb [photodynamic antimicrobial chemotherapy (pact) with light-emitting diode associated with toluidine blue solution and rmgic], and lmb ...201526502235
cariogram outcome after 90 days of oral treatment with streptococcus salivarius m18 in children at high risk for dental caries: results of a randomized, controlled study.dental caries is the most common chronic disease of childhood. cariogram is a well-recognized algorithm-based software program based on different caries-related risk factors and intended to aid clinicians in performing more objective and consistent dental caries risk assessments. this type of approach precedes the diagnosis of caries and allows the dentist to identify at-risk patients and then take appropriate preventive measures before caries develop further. one of the etiological factors favo ...201526491371
effect of enamel morphology on nanoscale adhesion forces of streptococcal bacteria : an afm study.we explore the influence of enamel surface morphology on nanoscale bacterial adhesion forces. three dimensional morphology characteristics of enamel slices, which were treated with phosphoric acid (for 0 s, 5 s, 10 s, 20 s, and 30 s), were acquired. adhesion forces of three initial colonizers (streptococcus oralis, streptococcus sanguinis, and streptococcus mitis) and two cariogenic bacterial strains (streptococcus mutans and streptococcus sobrinus) with etched enamel surfaces were determined. c ...201526482011
the microbiome in populations with a low and high prevalence of caries.the oral microbiota was compared between romanian adolescents with a high prevalence of caries and no dental care and swedish caries-active and caries-free adolescents in caries prevention programs and with a low prevalence of caries. biofilm samples were analyzed by flx+ pyrosequencing of the v1 to v4 hypervariable regions of the 16s rrna gene and polymerase chain reaction (pcr)/quantitative pcr (qpcr) for streptococcus mutans and streptococcus sobrinus. sequences obtained blasted to 9 phyla, 6 ...201626442950
inhibitory effects of arabitol on caries-associated microbiologic parameters of oral streptococci and lactobacilli.the aim of this study was to compare arabitol with its better studied isomer xylitol for their inhibitory effects on cell growth and acid production of oral bacteria. streptococcus mutans, streptococcus salivarius and streptococcus sobrinus were used as representatives of oral streptococci and lactobacillus acidophilus and lactobacillus fermentum were used for oral lactobacilli. growth was followed by measuring the absorbance at 660nm, acid production by ph change. sensitivity of these oral bact ...201526433188
longitudinal study of dental caries incidence associated with streptococcus mutans and streptococcus sobrinus in patients with intellectual disabilities.mutans streptococci (streptococcus mutans and s. sobrinus) are considered to be major etiologic agents of dental caries. using a polymerase chain reaction method, we detected those bacteria from 145 outpatients (6-30 years old) with intellectual disabilities (id) and their presence was compared with the incidence of dental caries.201526328921
in vitro antibacterial activity of nano silver ion substituted poly acrylic acid films on titanium by plasma polymerization.antibacterial activity of oral pathogens such as streptococcus mutans, streptococcus sobrinus when silver ion immobilized on commercially pure (cp) titanium (ti) surface was investigated in this study. plasma-polymerized acrylic acid to have carboxyl group was deposited on cp-ti surface and then ion-exchanged with ag+ ions in 0.1 n agno3. in anti-adherent experiment, antibacterial activity was tested using broth culture methods. the biofilm formation assay was performed using semi-defined biofil ...201526328382
oral streptococci biofilm formation on different implant surface topographies.the establishment of the subgingival microbiota is dependent on successive colonization of the implant surface by bacterial species. different implant surface topographies could influence the bacterial adsorption and therefore jeopardize the implant survival. this study evaluated the biofilm formation capacity of five oral streptococci species on two titanium surface topographies. in vitro biofilm formation was induced on 30 titanium discs divided in two groups: sandblasted acid-etched (sae- n = ...201526273590
in vitro growth-inhibitory effect of ethanol gras plant and supercritical co₂ hop extracts on planktonic cultures of oral pathogenic microorganisms.conventional chemical antiseptics used for treatment of oral infections often produce side-effects, which restrict their long-term use. plants are considered as perspective sources of novel natural antiseptics. however, little is still known about their inhibitory properties against oral pathogens. the objective of this study was to test in vitro antimicrobial activities of generally recognized as safe (gras) species against planktonic cultures of cariogenic, periodontal and candidal microorgani ...201526232134
antimicrobial activity of the essential oil of plectranthus neochilus against cariogenic bacteria.this work used the broth microdilution method to investigate the antimicrobial activity of the essential oil obtained from the leaves of plectranthus neochilus (pn-eo) against a representative panel of oral pathogens. we assessed the antimicrobial activity of this oil in terms of the minimum inhibitory concentration (mic). pn-eo displayed moderate activity against enterococcus faecalis (mic = 250 μg/ml) and streptococcus salivarus (mic = 250 μg/ml), significant activity against streptococcus sob ...201526161115
sol-gel-derived bioactive glasses demonstrate antimicrobial effects on common oral bacteria.to determine the antibacterial effect of nano-structured, sol-gel processed bioactive glasses that may be used for implants, coatings, and as adjuncts to dental restorative materials.201526087578
effects of baseplates of orthodontic appliances with in situ generated silver nanoparticles on cariogenic bacteria: a randomized, double-blind cross-over clinical trial.polymethyl-methacrylate (pmma) is commonly used primarily for baseplates of orthodontic appliances (boa). the activities of cariogenic bacteria in biofilm on these surfaces may contribute to dental caries, gingival inflammation and periodontal disease. the pmma incorporated with nanoparticles of silver (nanoag-i-pmma) and nanoag in situ in pmma (nanoag-is-pmma) have been shown to control the growth of cariogenic bacteria, but clinical trial of anti-cariogenic application of these novel materials ...201526067732
maturation of oral microbiota in children with or without dental caries.the aim of this longitudinal study was to evaluate the oral microbiota in children from age 3 months to 3 years, and to determine the association of the presence of caries at 3 years of age.201526020247
penetration of streptococcus sobrinus and streptococcus sanguinis into dental enamel.the aim of this pilot study was to assess the difference in virulence of acidogenic and aciduric oral streptococci in an in vitro caries model using their penetration depths into dental enamel. 30 caries-free extracted molars from 11- to 16-year-olds were cleaned ultrasonically for 1 min with de-ionized water and, after air-drying, embedded in epoxy resin. after 8-h of setting at room temperature, the specimens were ground on the buccal side with sic-paper 1200 (particle size 13-16 μm). enamel w ...201525805186
effect of rhus coriaria l. water extract on five common oral bacteria and bacterial biofilm formation on orthodontic wire.recently, use of herbal medicine and plant extracts as a substitute for commercially available chemical drugs for control of infectious diseases such as dental caries and periodontal disease has become increasingly popular. the present study was aimed to evaluate the effect of rhus coriaria l. water extract on five common oral bacteria and bacterial biofilm formation on orthodontic wire.201425802711
development of novel formulations containing lysozyme and lactoferrin and evaluation of antibacterial effects on mutans streptococci and lactobacilli.the aim of this study was to determine the antibacterial effect of different formulations containing lysozyme and lactoferrin and drug delivery system as well as poloxamer 407 with the trade name of pluronic f-127 and/or freeze dried liposome containing dotap [freeze dried liposomal dotap] on streptococcus sobrinus, streptococcus mutans and lactobacillus acidophilus in comparison with 0.2% chlorhexidine.201525757148
streptococcus mutans and streptococcus sobrinus colonization and caries experience in 3- and 5-year-old thai children.the aim of this study was to examine the colonization of streptococcus mutans and streptococcus sobrinus in supra-gingival plaque samples and to determine their correlation with the prevalence of early childhood caries (ecc) in thai children.201525753978
[antibacterial activity of synthetic antimicrobial decapeptide against oral bacteria].this study aims to evaluate the antimicrobial activity of decapeptide, a novel antimicrobial peptide, against several major cariogenic and periodontopathogenic bacteria in vitro. methods in this study, we investigated the antimicrobial activity of decapeptide against streptococcus mutans (s. mutans), streptococcus sobrinus, lactobacillus acidophilus, streptococcus sanguis, streptococcus gordonii, actinomyces viscosus, actinomyces naeslundii, porphyromonas gingivalis, prevotella intermedia, fusob ...201425665430
the corrosion resistance of wiron(®)88 in the presence of s. mutans and s. sobrinus bacteria.the corrosion resistance of wiron(®)88, a ni-cr-mo alloy, was evaluated in liquid growth media in the absence and presence of the streptococcus sobrinus and streptococcus mutans strains. open circuit potential measurements, cyclic voltammetry, linear sweep voltammetry, as well as electronic microscopy coupled to electron diffraction spectroscopy (sem/eds), were the main techniques used in this study. it was concluded that the presence of s. sobrinus and s. mutans have only a slight effect on the ...201525578707
effects of adjunctive daily phototherapy on chronic periodontitis: a randomized single-blind controlled trial.the purpose of this randomized single-blind controlled trial was to elucidate the clinical and antimicrobial effects of daily phototherapy (pt) as an adjunct to scaling and root planing (srp) in patients with chronic periodontitis.201425568808
rapid and sensitive pcr-dipstick dna chromatography for multiplex analysis of the oral microbiota.a complex of species has been associated with dental caries under the ecological hypothesis. this study aimed to develop a rapid, sensitive pcr-dipstick dna chromatography assay that could be read by eye for multiplex and semiquantitative analysis of plaque bacteria. parallel oligonucleotides were immobilized on a dipstick strip for multiplex analysis of target dna sequences of the caries-associated bacteria, streptococcus mutans, streptococcus sobrinus, scardovia wiggsiae, actinomyces species, ...201425485279
comparing the cariogenic species streptococcus sobrinus and s. mutans on whole genome level.two closely related species of mutans streptococci, namely streptococcus mutans and streptococcus sobrinus, are associated with dental caries in humans. their acidogenic and aciduric capacity is directly associated with the cariogenic potential of these bacteria. to survive acidic and temporarily harsh conditions in the human oral cavity with hundreds of other microbial co-colonizers as competitors, both species have developed numerous mechanisms for adaptation.201425475081
synergistic inhibition of streptococcal biofilm by ribose and xylitol.streptococcus mutans and streptococcus sobrinus are the major causative agents of human dental caries. therefore, the removal or inhibition of these streptococcal biofilms is essential for dental caries prevention. in the present study, we evaluated the effects of ribose treatment alone or in combination with xylitol on streptococcal biofilm formation for both species. furthermore, we examined the expression of genes responsible for dextran-dependent aggregation (ddag). in addition, we investiga ...201525463908
application of chimeric glucanase comprising mutanase and dextranase for prevention of dental biofilm formation.water-insoluble glucan (wig) produced by mutans streptococci, an important cariogenic pathogen, plays an important role in the formation of dental biofilm and adhesion of biofilm to tooth surfaces. glucanohydrolases, such as mutanase (α-1,3-glucanase) and dextranase (α-1,6-glucanase), are able to hydrolyze wig. the purposes of this study were to construct bi-functional chimeric glucanase, composed of mutanase and dextranase, and to examine the effects of this chimeric glucanase on the formation ...201525411090
synergistic effect of xylitol and ursolic acid combination on oral biofilms.this study was designed to evaluate the synergistic antibacterial effect of xylitol and ursolic acid (ua) against oral biofilms in vitro.201425383348
reducing dental plaque formation and caries development. a review of current methods and implications for novel pharmaceuticals.dental caries is an oral disease, which has a high worldwide prevalence despite the availability of various prophylactic means, including the daily use of fluoride toothpastes, water fluoridation, dental sealants, oral health educational programs and various antiseptic mouth-rinses. one important reason for this is uncontrolled increase in consumption of foods containing considerable sucrose concentration, especially among children. sucrose is easily metabolized by oral bacteria (mostly streptoc ...201425209226
nanoparticle-encapsulated chlorhexidine against oral bacterial biofilms.chlorhexidine (chx) is a widely used antimicrobial agent in dentistry. herein, we report the synthesis of a novel mesoporous silica nanoparticle-encapsulated pure chx (nano-chx), and its mechanical profile and antimicrobial properties against oral biofilms.201425170958
characterization and bacterial anti-adherent effect on modified pmma denture acrylic resin containing platinum nanoparticles.this study characterized the synthesis of a modified pmma (polymethyl methacrylate) denture acrylic loading platinum nanoparticles (ptn) and assessed its bacterial inhibitory efficacy to produce novel antimicrobial denture base material.201425006385
[analysis of caries susceptibility factors during transition from caries-free to caries in three-year-old children].to investigate the changes of caries susceptibility factors during the transition from caries-free to caries in three-year-old children.201424969591
antibacterial activities of extracts from ugandan medicinal plants used for oral care.medicinal plants are widely used for treatment of oral/dental diseases in uganda.201424945400
inhibitory effects of a cured antibacterial bonding system on viability and metabolic activity of oral bacteria.to evaluate the antimicrobial efficacy of clearfil se protect (cp) and clearfil se bond (cb) after curing and rinsed against five individual oral microorganisms as well as a mixture of bacterial culture prepared from the selected test organisms.201424880721
mutans streptococci enumeration and genotype selection using different bacitracin-containing media.the primary etiological agents associated with dental caries include the mutans streptococci (ms) comprised of streptococcus mutans and streptococcus sobrinus. the effective cultivation and isolation of ms are necessary for the study of ms, including their proper clinical assessment in the epidemiological study of dental caries. several selective media have been developed for the isolation, enumeration, and characterization of ms. however, inhibition of ms may occur, reducing counts and perhaps ...201424878141
inhibitory effect of ti-ag alloy on artificial biofilm formation.titanium-silver (ti-ag) alloy has been improved for machinability and mechanical properties, but its anti-biofilm properties have not been elucidated yet. thus, this study aimed to evaluate the effects of ti-ag alloy on biofilm formation and bacterial viability in comparison with pure ti, pure ag and silver-palladium (ag-pd) alloy. biofilm formation on the metal plates was evaluated by growing streptococcus mutans and streptococcus sobrinus in the presence of metal plates. bactericidal activity ...201424786344
quantitative analysis of changes in salivary mutans streptococci after orthodontic treatment.the purpose of this study was to analyze the initial changes in salivary mutans streptococci levels after orthodontic treatment with fixed appliances.201424785924
bacterial profiles of saliva in relation to diet, lifestyle factors, and socioeconomic status.the bacterial profile of saliva is composed of bacteria from different oral surfaces. the objective of this study was to determine whether different diet intake, lifestyle, or socioeconomic status is associated with characteristic bacterial saliva profiles.201424765243
composition and activity against oral pathogens of the essential oil of melampodium divaricatum (rich.) dc.the chemical composition of the essential oil isolated from the aerial parts of melampodium divaricatum (rich.) dc. (asteraceae) was characterized by gc-fid and gc/ms analyses. (e)-caryophyllene (56.0%), germacrene d (12.7%), and bicyclogermacrene (9.2%) were identified as the major oil components. the antimicrobial activity of the oil against seven standard strains of oral pathogens from the american type culture collection (atcc) was evaluated by determining minimum inhibitory concentrations ( ...201424634073
effect of silver ion coating of fixed orthodontic retainers on the growth of oral pathogenic bacteria.titanium and stainless steel wires used for retainers in orthodontic procedures were coated with ag ions and the effects of the coating on common oral pathogens and their pathogenicity were investigated. two species of cariogenic and three species of periodontopathic bacteria were assessed. biofilms of streptococcus sobrinus and two vsc gases produced by p. gingivalis were also examined. ag ion-coated wires showed marked antibacterial activities compared with uncoated wires; in most cases, the d ...201424614999
systematic screening of plant extracts from the brazilian pantanal with antimicrobial activity against bacteria with cariogenic relevance.this study proposes a bioprospection methodology regarding the antimicrobial potential of plant extracts against bacteria with cariogenic relevance. sixty extracts were obtained from ten plants--(1) jatropha weddelliana, (2) attalea phalerata, (3) buchenavia tomentosa, (4) croton doctoris, (5) mouriri elliptica, (6) mascagnia benthamiana, (7) senna aculeata, (8) unonopsis guatterioides, (9) allagoptera leucocalyx and (10) bactris glaucescens--using different extraction methods - (a) 70° ethanol ...201424603299
investigations of kanuka and manuka essential oils for in vitro treatment of disease and cellular inflammation caused by infectious microorganisms.diseases caused by infectious and inflammatory microorganisms are among the most common and most severe nosocomial diseases worldwide. therefore, developing effective agents for treating these illnesses is critical. in this study, essential oils from two tea tree species, kanuka (kunzea ericoides) and manuka (leptospermum scoparium), were evaluated for use in treating diseases and inflammation caused by microorganism infection.201624582465
comparative short-term in vitro analysis of mutans streptococci adhesion on esthetic, nickel-titanium, and stainless-steel arch wires.to test the hypothesis that there are no differences in mutans streptococci (ms) adhesion between esthetic and metallic orthodontic arch wires based on their surface characteristics.201424308530
antimicrobial activity of fractions and subfractions of elaeagia utilis against microorganisms of importance in dental caries.dental caries is a multifactorial infectious disease that leads to the destruction of dental hard tissue. the main goal of research into medicinal plants is to seek compounds with antimicrobial activity for subsequent use in prevention strategies and control of infectious diseases. the aim of this study was to evaluate the antimicrobial activity of fractions and subfractions obtained from elaeagia utilis against streptococcus mutans, streptococcus sobrinus and lactobacillus acidophilus. the plan ...201324303734
screening and scoring of antimicrobial and biological activities of italian vulnerary plants against major oral pathogenic bacteria.this study aims to evaluate the activity of italian vulnerary plants against the most important oral pathogenic bacteria. this estimate was accomplished through a fivefold process: (a) a review of ethnobotanical and microbiological data concerning the italian vulnerary plants; (b) the development of a scoring system to rank the plants; (c) the comparative assessment of microbiological properties; (d) the assessment of potential cytotoxic effects on keratinocyte-like cells and gingival fibroblast ...201324302963
chemical and botanical characterization of chilean propolis and biological activity on cariogenic bacteria streptococcus mutans and streptococcus sobrinus.propolis is a non-toxic natural substance with multiple pharmacological properties including anti-cancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. the aim of this study was to determine the chemical and botanical characterization of chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria streptococcus mutans and streptococcus sobrinus. twenty propolis samples were obtained from beekeeping producers from the ...201324294257
frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity.one of the causative factors in development of dental caries is microorganisms. two species of mutans streptococci including streptococcus mutans and streptococcus sobrinus are associated with dental caries in human beings. the aim of this study was to investigate the frequency of s. mutans and s. sobrinus in saliva of children with different caries activity and ability to form biofilm and acid susceptibility of these microorganisms.201324130577
[the relationship between streptococcus sobrinus and rampant caries in children].to explore the relations between streptococcus sobrinus (s. sobrinus) and rampant caries in children.201324100904
antimicrobial activity of isothiocyanates (itcs) extracted from horseradish (armoracia rusticana) root against oral microorganisms.the antimicrobial activity of isothiocyanates (itcs) extracted from horseradish root was investigated against oral microorganisms: 6 strains of facultative anaerobic bacteria, streptococcus mutans, streptococcus sobrinus, lactobacillus casei, staphylococcus aureus, enterococcus faecalis and aggregatibacter actinomycetemcomitans; one strain of yeast, candida albicans, and 3 strains of anaerobic bacteria, fusobacterium nucleatum, prevotella nigrescens, and clostridium perfringens. the itcs extract ...201324077540
analysis of cariogenic bacteria in saliva of cancer patients.this study examined salivary flow and salivary ph and the prevalence and levels of cariogenic bacteria in the saliva of oncological patients and healthy controls. quantitative real-time polymerase chain reaction was used to assess the levels of microbes including streptococcus mutans, streptococcus sobrinus, lactobacillus salivarius, and lactobacillus acidophilus in the saliva of 41 patients with a solid tumor (so), 30 patients with a hematologic malignancy (he), and 40 healthy controls. salivar ...201324010070
functional ginger extracts from supercritical fluid carbon dioxide extraction via in vitro and in vivo assays: antioxidation, antimicroorganism, and mice xenografts models.supercritical fluid carbon dioxide extraction technology was developed to gain the active components from a taiwan native plant, zingiber officinale (ginger). we studied the biological effects of ginger extracts via multiple assays and demonstrated the biofunctions in each platform. investigations of ginger extracts indicated antioxidative properties in dose-dependant manners on radical scavenging activities, reducing powers and metal chelating powers. we found that ginger extracts processed mod ...201323983624
characterization and in vitro properties of oral lactobacilli in breastfed infants.lactobacillus species can contribute positively to general and oral health and are frequently acquired by breastfeeding in infancy. the present study aimed to identify oral lactobacilli in breast and formula-fed 4 month-old infants and to evaluate potential probiotic properties of the dominant lactobacillus species detected. saliva and oral swab samples were collected from 133 infants who were enrolled in a longitudinal study (n=240) examining the effect of a new infant formula on child growth a ...201323945215
inhibition of initial adhesion of oral bacteria through a lectin from bauhinia variegata l. var. variegata expressed in escherichia coli.the aim of the present work was to study the in vitro effect of native and recombinant bauhinia variegata var. variegata lectins in inhibiting early adhesion of streptococcus mutans, streptococcus sanguis and streptococcus sobrinus to experimentally acquired pellicle.201323910219
effects of 2 bracket and ligation types on plaque retention: a quantitative microbiologic analysis with real-time polymerase chain reaction.the aim of this study was to evaluate the effects of self-ligating brackets and conventional brackets ligated with stainless steel ligatures on dental plaque retention and microbial flora.201323910207
genotypic diversity and virulence traits of streptococcus sobrinus isolated from caries-free children and children suffering severe early childhood caries.to evaluate the genotypic diversity and some virulence traits of streptococcus sobrinus (s. sobrinus) isolated from caries-free children and children suffering severe early childhood caries (secc).201323878828
monitoring the prevalence of viable and dead cariogenic bacteria in oral specimens and in vitro biofilms by qpcr combined with propidium monoazide.streptococcus mutans and streptococcus sobrinus are associated with the development of dental caries in humans. however, previous diagnostic systems are unsuitable for monitoring viable cell numbers in oral specimens. assessing the relationship between the numbers of viable and dead bacterial cells and oral status is important for understanding oral infectious diseases. propidium monoazide (pma) has been reported to penetrate dead cells following membrane damage and to cross-link dna, thereby in ...201323848601
lab-test(®) 4: dental caries and bacteriological analysis.dental caries is one of the most common infectious ultifactorial diseases worldwide, characterized by the progressive demineralization of the tooth, following the action of bacterial acid metabolism. the main factors predisposing the onset of the carious process are: 1) the presence of bacterial species able to lower the ph until critical values of 5.5, 2) the absence of adequate oral hygiene, 3) an inefficient immune response anti-caries, 4) the type of alimentary diet and 5) the structure of t ...201223814571
salivary iga response to probiotic bacteria and mutans streptococci after the use of chewing gum containing lactobacillus reuteri.we investigated whether ingestion of probiotic bacteria could influence salivary iga levels, specific anti-mutans streptococci iga levels and specific antibodies towards the ingested probiotic bacterium. the study was a randomised, double-blind, placebo-controlled trial, where the test group (n = 11) received twice daily chewing of gum containing lactobacillus reuteri (2 × 10(8)  cfu per dose) and the control group (n = 12) received placebo. resting saliva was collected before and after 12 weeks ...201323737255
streptococcus mutans and streptococcus sobrinus detection by polymerase chain reaction and their relation to dental caries in 12 and 15 year-old schoolchildren in valencia (spain).a cross-sectional study was carried out to determine the prevalence of streptococcus mutans and streptococcus sobrinus and the association of the two in a random sample (n=614) of the child population of the region of valencia (spain). saliva samples were analyzed by the quantitative polymerase chain reaction (pcr) method to study the relation of these bacteria to caries prevalence and the dmft index. the prevalence of s. mutans was 35.4% at age 12 and 22.9% at age 15, that of s. sobrinus 18.9% ...201323722138
distribution of streptococcus troglodytae and streptococcus dentirousetti in chimpanzee oral cavities.the aim of this study was to analyze the distribution and phenotypic properties of the indigenous streptococci in chimpanzee (pan troglodytes) oral cavities. eleven chimpanzees (aged from 9 to 44 years, mean ± sd, 26.9 ± 12.6 years) in the primate research institute of kyoto university were enrolled in this research and brushing bacterial samples collected from them. streptococci were isolated from the oral cavities of all chimpanzees. the isolates (n = 46) were identified as thirteen species by ...201323668608
anti-inflammatory and antimicrobial evaluation of neovestitol and vestitol isolated from brazilian red propolis.the objective of this study was to evaluate anti-inflammatory and antimicrobial activities of neovestitol and vestitol isolated from brazilian red propolis (brp). brp ethanolic extract (eep), neovestitol, and vestitol were evaluated by anti-inflammatory properties using a neutrophil migration assay. the antimicrobial activity was evaluated by minimal inhibitory and bactericidal concentrations (mic and mbc) against streptococcus mutans , streptococcus sobrinus , staphylococcus aureus , and actino ...201323607483
chitosan-thioglycolic acid as a versatile antimicrobial agent.as functionalized chitosans hold great potential for the development of effective and broad-spectrum antibiotics, representative chitosan derivatives were tested for antimicrobial activity in neutral media: trimethyl chitosan (tmc), carboxy-methyl chitosan (cmc), and chitosan-thioglycolic acid (tga; medium molecular weight: mmw-tga; low molecular weight: lmw-tga). colony forming assays indicated that lmw-tga displayed superior antimicrobial activity over the other derivatives tested: a 30 min in ...201323470196
antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries.silver diamine fluoride (sdf) has clinical success in arresting dentin caries, this study aimed to investigate its mechanism of action.201323442825
adhesion of streptococci to various orthodontic composite resins.this investigation aimed to determine quantitatively the adhesion of streptococcus mutans and streptococcus sobrinus to orthodontic composite resins that were tested simultaneously using radio-markers.201323441799
antimicrobial activity of the synthetic peptide lys-a1 against oral streptococci.the peptide lys-[trp(6)]-hy-a1 (lys-a1) is a synthetic derivative of the peptide hy-a1, initially isolated from the frog species hypsiboas albopunctatus. according to previous research, it is a molecule with broad antimicrobial activity. the objective of this study was to evaluate the antimicrobial activity of the synthetic peptide lys-a1 (kifgaiwplalgalknlik-nh2) on the planktonic and biofilm growth of oral bacteria. the methods used to evaluate antimicrobial activity include the following: det ...201323340019
[effect of lemon essential oil on caries factors of streptococcus sobrinus].to investigate the mechanism of lemon peel essential oil (lpe) on the cariogenicity of streptococcus sobrinus (ss).201223328100
enantiomeric hplc resolution and absolute stereochemistry assignment of a new poligamain derivative.a new aryltetralin lignan derivative, 1, was obtained by reacting dimethyl succinate and piperonal, furnishing the lactone 4-(3',4'-methylenedioxybenzyl)-4,5-dihydro-2(3h)-furanone, which was reacted once again with piperonal and lda to give the dibenzylbutirolactone 7-hydroxyhinokinin. the cyclization of 7-hydroxyhinokinin into polygamain occurred in the presence of trifluoroacetic acid. the reduction of the furanic ring of polygamain was done by its reaction with dibal in thf, furnishing the d ...201223312389
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