Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| activation of invariant natural killer t cells stimulated with microbial α-mannosyl glycolipids. | some synthetic and bacterial glycolipids presented by cd1d specifically activate invariant nkt (inkt) cells bearing an invariant vα14-jα18 (mouse) or vα24-jα18 (human) tcr. the antigenic glycolipids identified to date consist of two hydrophobic chains and an α-glycoside in which the 2'-oh group is in the cis orientation toward the anomeric group, namely, either an α-galactoside or an α-glucoside. several microbial α-mannosyl glycolipids, in which the 2'-oh group is in the trans orientation, were ... | 2017 | 28852174 |
| hsds, belonging to the type i restriction-modification system, contributes to the streptococcus suis serotype 2 survival ability in phagocytes. | streptococcus suis serotype 2 (ss2) is an important zoonotic agent in swine and humans. anti-phagocytosis and survival in phagocytic cells and whole blood is essential for bacteria to be pathogenic. in this study, the host specificity determinant specificity subunit (coded by hsds) of the type i restriction-modification system and two peptidoglycan-binding proteins (coded by lysm and lysm', respectively), which were simultaneously found to be subjected to transcript-level influence by hsds, were ... | 2017 | 28848531 |
| defining the taxonomic status of streptococcus suis serotype 33: the proposal for streptococcus ruminantium sp. nov. | to clarify the taxonomic classification of streptococcus suis serotype 33, we performed biochemical and molecular genetic analyses using isolates (gut-183, gut-184, gut-185, gut-186, gut-187t, gut-188, gut-189, gut-190, gut-191, gut-192 and gut-193) from bovine endocarditis. a comparative sequence analysis showed 99.2-100 % sequence similarity among the reference strain of s. suis serotype 33 and our isolates for the 16s rrna gene. these similarities were higher than those between the isolate gu ... | 2017 | 28840807 |
| hp1330 contributes to streptococcus suis virulence by inducing toll-like receptor 2- and erk1/2-dependent pro-inflammatory responses and influencing in vivo s. suis loads. | streptococcus suis 2 (ss2) has evolved into a highly invasive pathogen responsible for two large-scale outbreaks of streptococcal toxic shock-like syndrome (stsls) in china. excessive inflammation stimulated by ss2 is considered a hallmark of stsls, even it also plays important roles in other clinical symptoms of ss2-related disease, including meningitis, septicemia, and sudden death. however, the mechanism of ss2-caused excessive inflammation remains poorly understood. here, a novel pro-inflamm ... | 2017 | 28824616 |
| characterization of a novel streptococcal heme-binding protein snta and its interaction with host antioxidant protein aop2. | efficient iron acquisition is critical for bacteria's survival, virulence and pathology of diseases. the largest reservoir of iron in mammals is incorporated into heme, which can be acquired by bacterial pathogens as a nutritional iron source. in this study, a cell wall protein snta of streptococcus suis serotype 2 (ss2) was characterized as a novel heme-binding protein by using the pyridine hemochrome assay and icp-ms measurement. yeast two-hybrid and pull-down assays revealed that the snta pro ... | 2017 | 28823793 |
| patterns of antimicrobial resistance in streptococcus suis isolates from pigs with or without streptococcal disease in england between 2009 and 2014. | antimicrobial resistance in streptococcus suis, a global zoonotic pathogen of pigs, has been mostly studied only in diseased animals using surveys that have not evaluated changes over time. we compared patterns of resistance between s. suis isolates from clinical cases of disease (cc) and non-clinical case (ncc) pigs in england, collected over two discrete periods, 2009-2011 and 2013-2014. minimum inhibitory concentrations (mic) of 17 antimicrobials (nine classes) were determined on 405 s. suis ... | 2017 | 28757010 |
| the bias of experimental design, including strain background, in the determination of critical streptococcus suis serotype 2 virulence factors. | streptococcus suis serotype 2 is an important porcine bacterial pathogen and emerging zoonotic agent mainly responsible for sudden death, septic shock, and meningitis. however, serotype 2 strains are genotypically and phenotypically heterogeneous. though a multitude of virulence factors have been described for s. suis serotype 2, the lack of a clear definition regarding which ones are truly "critical" has created inconsistencies that have only recently been highlighted. herein, the involvement o ... | 2017 | 28753679 |
| sub-minimum inhibitory concentrations of rhubarb water extracts inhibit streptococcus suis biofilm formation. | streptococcus suis is one of the most important swine pathogens, which can cause persistent infection by forming biofilms. in this study, sub-minimum inhibitory concentration (sub-mic) of rhubarb water extracts were found to inhibit biofilm formation. two-component signal transduction systems (tcss), transcriptional regulators, and dna binding proteins were compared under two conditions: (1) cells treated with sub-mic rhubarb water extracts and (2) untreated cells. using an isobaric tags for rel ... | 2017 | 28736523 |
| seven new lignan glycosides from the branches of alangium kurzii craib var. laxifolium. | seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ... | 2017 | 28733150 |
| a positive feedback loop mediated by sigma x enhances expression of the streptococcal regulator comr. | natural transformation is used by bacteria to take up dna from their surroundings and incorporate it into their genomes. streptococci do so during a transient period of competence, triggered by pheromones that they produce, secrete and sense under conditions influenced by the environment. in streptococcus mutans, streptococcus suis, and species of the bovis, salivarius and pyogenic groups of streptococci, the pheromone xip is sensed by the intra-cellular regulator comr, that in turn activates th ... | 2017 | 28729683 |
| impact of a food safety campaign on streptococcus suis infection in humans in thailand. | abstractstreptococcus suis is an important zoonotic pathogen in swine and humans that causes sepsis and meningitis. our previous study in thailand showed that the prevalence of s. suis infection in humans, especially in northern areas of thailand, and the transmission of the pathogen occurred mainly through the consumption of traditional raw pork products. considering the high incidence proportion and mortality rate of the disease as an important public health problem, we implemented a food safe ... | 2017 | 28719258 |
| natural killer cell functions during the innate immune response to pathogenic streptococci. | dendritic cells (dcs) and nk cells play a crucial role in the first phase of host defense against infections. group b streptococcus (gbs) and streptococcus suis are encapsulated streptococci causing severe systemic inflammation, leading to septicemia and meningitis. yet, the involvement of nk cells in the innate immune response to encapsulated bacterial infection is poorly characterized. here, it was observed that these two streptococcal species rapidly induce the release of ifn-γ and that nk ce ... | 2017 | 28706510 |
| serotype-specific role of antigen i/ii in the initial steps of the pathogenesis of the infection caused by streptococcus suis. | streptococcus suis is one of the most important post-weaning porcine bacterial pathogens worldwide. the serotypes 2 and 9 are often considered the most virulent and prevalent serotypes involved in swine infections, especially in europe. however, knowledge of the bacterial factors involved in the first steps of the pathogenesis of the infection remains scarce. in several pathogenic streptococci, expression of multimodal adhesion proteins known as antigen i/ii (agi/ii) have been linked with persis ... | 2017 | 28705175 |
| bacterial bloodstream infections in a tertiary infectious diseases hospital in northern vietnam: aetiology, drug resistance, and treatment outcome. | bloodstream infections (bsis) are associated with high morbidity and mortality worldwide. however their aetiology, antimicrobial susceptibilities and associated outcomes differ between developed and developing countries. systematic data from vietnam are scarce. here we present aetiologic data on bsi in adults admitted to a large tertiary referral hospital for infectious diseases in hanoi, vietnam. | 2017 | 28701159 |
| erratum to: genome-wide analysis of an avirulent strain that induces protective immunity against challenge with virulent streptococcus suis serotype 2. | 2017 | 28676041 | |
| microbiological zoonotic emerging risks, transmitted between livestock animals and humans (2007-2015). | as part of the emerging risk identification (eri) activities of the european food safety authority (efsa), a literature search was conducted to identify the microbiological agents transmitted between livestock animals and humans that have been suggested as having emerged between 2007 and 2015 in peer-reviewed scientific literature published during the same period (2007-2015). according to the criteria set, the search identified seven such zoonotic agents, namely west nile fever virus, rift valle ... | 2017 | 28670863 |
| rutin inhibits streptococcus suis biofilm formation by affecting cps biosynthesis. | streptococcus suis (s. suis) form biofilms and causes severe diseases in humans and pigs. biofilms are communities of microbes embedded in a matrix of extracellular polymeric substances. eradicating biofilms with the use of antibiotics or biocides is often ineffective and needs replacement with other potential agents. compared to conventional agents, promising and potential alternatives are biofilm-inhibiting compounds without impairing growth. here, we screened a s. suis adhesion inhibitor, rut ... | 2017 | 28670278 |
| a single amino acid polymorphism in the glycosyltransferase cpsk defines four streptococcus suis serotypes. | the capsular polysaccharide (cps) is the major virulence factor of the emerging zoonotic pathogen streptococcus suis. cps differences are also the basis for serological differentiation of the species into 29 serotypes. serotypes 2 and 1/2, which possess identical gene content in their cps loci, express cpss that differ only by substitution of galactose (gal) by n-acetylgalactosamine (galnac) in the cps side chain. the same sugar substitution differentiates the cps of serotypes 14 and 1, whose cp ... | 2017 | 28642597 |
| preferential aerosolization of bacteria in bioaerosols generated in vitro. | little is known about how bacteria are aerosolized in terms of whether some bacteria will be found in the air more readily than others that are present in the source. this report describes in vitro experiments to compare aerosolization rates (also known as preferential aerosolization) of gram-positive and gram-negative bacteria as well as rod- and coccus-shaped bacteria, using two nebulization conditions. | 2017 | 28632907 |
| the manganese efflux system mnte contributes to the virulence of streptococcus suis serotype 2. | manganese is an essential micronutrient to bacteria and plays an important role in bacterial physiology. however, an excess of manganese is extremely deleterious to the cell. the manganese efflux system is used to control intracellular manganese levels by some bacteria. in this study, we have identified a cation efflux family protein (mnte) that functions as a manganese export system in streptococcus suis serotype 2. to investigate the role of mnte in s. suis 2, a mnte deletion mutant (δmnte) an ... | 2017 | 28629722 |
| streptococcus suis serotype 9 strain gz0565 contains a type vii secretion system putative substrate esxa that contributes to bacterial virulence and a vanz-like gene that confers resistance to teicoplanin and dalbavancin in streptococcus agalactiae. | streptococcus suis (ss), an important pathogen for pigs, is not only considered as a zoonotic agent for humans, but is also recognized as a major reservoir of antimicrobial resistance contributing to the spread of resistance genes to other pathogenic streptococcus species. in addition to serotype 2 (ss2), serotype 9 (ss9) is another prevalent serotype isolated from diseased pigs. although many ss strains have been sequenced, the complete genome of a non-ss2 virulent strain has been unavailable t ... | 2017 | 28622857 |
| the streptococcus suis factor h-binding protein: a key to unlocking the blood-brain barrier and access the central nervous system? | 2017 | 28622084 | |
| prevalence of multiple drug resistant streptococcus suis in and around guwahati, india. | this study was conducted to determine the prevalence and antimicrobial susceptibility of streptococcus suis and their resistance patterns isolated from both clinically healthy carriers and diseased pigs in and around guwahati, assam, india. | 2017 | 28620262 |
| comparison of asian porcine high fever disease isolates of porcine reproductive and respiratory syndrome virus to united states isolates for their ability to cause disease and secondary bacterial infection in swine. | epidemiologic data from asian outbreaks of highly-pathogenic (hp) porcine reproductive and respiratory syndrome virus (prrsv) suggest that disease severity was associated with both the virulence of the prrsv isolates and secondary bacterial infections. previous reports have indicated that u.s. isolates of prrsv predispose to secondary bacterial infections as well, but the severity of disease that occurred in asia in pigs infected with these hp-prrsv strains has not been reported in the u.s. the ... | 2017 | 28619168 |
| pathogen-reactive t helper cell analysis in the pig. | there is growing interest in studying host-pathogen interactions in human-relevant large animal models such as the pig. despite the progress in developing immunological reagents for porcine t cell research, there is an urgent need to directly assess pathogen-specific t cells-an extremely rare population of cells, but of upmost importance in orchestrating the host immune response to a given pathogen. here, we established that the activation marker cd154 (cd40l), known from human and mouse studies ... | 2017 | 28567041 |
| differences in microbiota membership along the gastrointestinal tract of piglets and their differential alterations following an early-life antibiotic intervention. | early-life antibiotic interventions can change the predisposition to disease by disturbing the gut microbiota. however, the impact of antibiotics on gut microbiota in the gastrointestinal tract is not completely understood, although antibiotic-induced alterations in the distal gut have been reported. here, employing a piglet model, the microbial composition was analyzed by high-throughput 16s rrna gene sequencing and picrust predictions of metagenome function. the present study showed clear spat ... | 2017 | 28536561 |
| potentially hazardous streptococcus suis strains latent in asymptomatic pigs in a major swine production area of thailand. | carrier pigs have been considered as the major reservoir of streptococcus suis and couldbe a significant source of human infection. therefore, we investigated the prevalence and characteristics of latent s. suis in asymptomatic pigs in the pig-farming area of central thailand, and compared the data to those previously reported in other regions. | 2017 | 28516843 |
| draft genome sequence of clinical strain tani1 of streptococcus suis serotype 5 isolated from a bacteremia patient in japan. | streptococcus suis is a swine pathogen that causes severe economic damage to the porcine industry. it occasionally evokes zoonotic infection in humans. here, we report a draft genome sequence of a s. suis serotype 5 strain isolated from a bacteremia patient that was reported by taniyama et al. (d. taniyama, m. sakurai, t. sakai, t. kikuchi, and t. takahashi, idcases 6:36-38, 2016, https://doi.org/10.1016/j.idcr.2016.09.011). | 2017 | 28473377 |
| muramidase-released protein of streptococcus suis: new insight into its impact on virulence. | 2017 | 28467144 | |
| expression and characterization of serotype 2 streptococcus suis arginine deiminase. | arginine deiminase (arca) has been speculated to facilitate the intracellular survival of streptococcus suis under acidic conditions. however, the physical and biological properties and function of ss2-arca have not yet been elucidated. | 2017 | 28456803 |
| evaluation of the immunomodulatory properties of streptococcus suis and group b streptococcus capsular polysaccharides on the humoral response. | streptococcus suis and group b streptococcus (gbs) are encapsulated streptococci causing septicemia and meningitis. antibodies (abs) against capsular polysaccharides (cpss) have a crucial protective role, but the structure/composition of the cps, including the presence of sialic acid, may interfere with the generation of anti-cps ab responses. we investigated the features of the cps-specific ab response directed against s. suis serotypes 2 and 14 and gbs serotypes iii and v after infection or im ... | 2017 | 28425925 |
| streptococcal adhesin p (sadp) contributes to streptococcus suis adhesion to the human intestinal epithelium. | streptococcus suis is a zoonotic pathogen, causing meningitis and septicemia. we previously demonstrated that the gastrointestinal tract (git) is an entry site for zoonotic s. suis infection. here we studied the contribution of streptococcal adhesin protein (sadp) to host-pathogen interaction at git level. | 2017 | 28407026 |
| interaction of factor h-binding protein of streptococcus suis with globotriaosylceramide promotes the development of meningitis. | streptococcus suis is an important emerging zoonotic agent that causes acute bacterial meningitis in humans with high mortality and morbidity. our previous work showed that factor h-binding protein (fhb) contributed to virulence of s. suis, but the role of fhb in the development of s. suis meningitis remained unclear. in this study, we demonstrated for the first time that fhb contributed to the traversal of s. suis across the human blood-brain barrier by allelic-exchange mutagenesis, complementa ... | 2017 | 28402705 |
| a locus encoding variable defence systems against invading dna identified in streptococcus suis. | streptococcus suis, an important zoonotic pathogen, is known to have an open pan-genome and to develop a competent state. in s. suis, limited genetic lineages are suggested to be associated with zoonosis. however, little is known about the evolution of diversified lineages and their respective phenotypic or ecological characteristics. in this study, we performed comparative genome analyses of s. suis, with a focus on the competence genes, mobile genetic elements (mges), and genetic elements rela ... | 2017 | 28379509 |
| streptococcus suis serotype 2 biofilms inhibit the formation of neutrophil extracellular traps. | invasive infections caused by streptococcus suis serotype 2 (ss2) has emerged as a clinical problem in recent years. neutrophil extracellular traps (nets) are an important mechanism for the trapping and killing of pathogens that are resistant to phagocytosis. biofilm formation can protect bacteria from being killed by phagocytes. until now, there have only been a few studies that focused on the interactions between bacterial biofilms and nets. ss2 in both a biofilm state and a planktonic cell st ... | 2017 | 28373968 |
| morin attenuates streptococcus suis pathogenicity in mice by neutralizing suilysin activity. | streptococcus suis, a gram-positive pathogen, is widely recognized as an important agent of swine infection, and it is also known to cause a variety of zoonoses, such as meningitis, polyarthritis and pneumonia. suilysin (sly), an extracellular pore-forming toxin that belongs to the cholesterol-dependent cytolysin family, is an essential virulence factor of s. suis capsular type 2 (ss2). here, we found that morin hydrate (morin), a natural flavonoid that lacks anti-ss2 activity, inhibits the hemo ... | 2017 | 28373868 |
| the non-conserved region of mrp is involved in the virulence of streptococcus suis serotype 2. | muramidase-released protein (mrp) of streptococcus suis serotype 2 (ss2) is an important epidemic virulence marker with an unclear role in bacterial infection. to investigate the biological functions of mrp, three mutants named δmrp, δmrp domain 1 (δmrp-d1), and δmrp domain 2 (δmrp-d2) were constructed to assess the phenotypic changes between the parental strain and the mutant strains. the results indicated that mrp domain 1 (mrp-d1, the non-conserved region of mrp from a virulent strain, a.a. 2 ... | 2017 | 28362221 |
| streptococcus suis serotype 2 strains isolated in argentina (south america) are different from those recovered in north america and present a higher risk for humans. | streptococcus suis serotype 2 is an important swine pathogen and emerging zoonotic agent causing meningitis and septicemia/septic shock. strains are usually virulent (eurasia) or of intermediate/low virulence (north america). very few data regarding human and swine isolates from south america are available. | 2016 | 28348788 |
| survey of susceptibility to marbofloxacin in bacteria isolated from diseased pigs in europe. | a monitoring programme of marbofloxacin susceptibility of bacteria from europe causing respiratory tract infection and meningitis in pigs has been active since 1994 and 2002, respectively. monitoring digestive, metritis and urinary tract infection (uti) in pigs has been active since 2005 and susceptibility results until 2013 are presented. minimum inhibitory concentration (mic) was determined by broth microdilution. for mic interpretation, vétoquinol-evaluated breakpoints were applied. for diges ... | 2017 | 28348142 |
| porcine dendritic cells as an in vitro model to assess the immunological behaviour of streptococcus suis subunit vaccine formulations and the polarizing effect of adjuvants. | an in vitro porcine bone marrow-derived dendritic cell (dc) culture was developed as a model for evaluating immune polarization induced by adjuvants when administered with immunogens that may become vaccine candidates if appropriately formulated. the swine pathogen streptococcus suis was chosen as a prototype to evaluate proposed s. suis vaccine candidates in combination with the adjuvants poly i:c, quil a ®, alhydrogel ®, titermax gold ® and stimune ®. the toll-like receptor ligand poly i:c and ... | 2017 | 28327531 |
| the eukaryote-like serine/threonine kinase stk regulates the growth and metabolism of zoonotic streptococcus suis. | like eukaryotes, bacteria express one or more serine/threonine kinases (stks) that initiate diverse signaling networks. the stk from streptococcus suis is encoded by a single-copy stk gene, which is crucial in stress response and virulence. to further understand the regulatory mechanism of stk in s. suis, a stk deletion strain (δstk) and its complementary strain (cδstk) were constructed to systematically decode stk characteristics by applying whole transcriptome rna sequencing (rna-seq) and phos ... | 2017 | 28326294 |
| streptococcus suis sortase a is ca2+ independent and is inhibited by acteoside, isoquercitrin and baicalin. | sortase a (srta) has long been recognized as an ideal drug target for therapeutic agents against gram-positive pathogens. however, the srta of streptococcus suis (ss-srta), an important zoonotic agent, has not been studied. in this study, the enzymatic properties of ss-srta were investigated, and inhibition of ss-srta by natural products was evaluated. ss-srta was expressed and purified. the purified recombinant ss-srta had maximal activity at ph 6.0-7.5, 45°c, and showed a km of 6.7 μm for the ... | 2017 | 28319184 |
| better understanding of homologous recombination through a 12-week laboratory course for undergraduates majoring in biotechnology. | homologous recombination, a central concept in biology, is defined as the exchange of dna strands between two similar or identical nucleotide sequences. unfortunately, undergraduate students majoring in biotechnology often experience difficulties in understanding the molecular basis of homologous recombination. in this study, we developed and implemented a 12-week laboratory course for biotechnology undergraduates in which gene targeting in streptococcus suis was used to facilitate their underst ... | 2017 | 28314074 |
| genome-wide analysis of a avirulent and reveal the strain induces pro-tective immunity against challenge with virulent streptococcus suis serotype 2. | it was previously reported in china that two recent large-scale outbreaks of streptococcus suis serotype 2 (s. suis 2) infections in human were caused by two highly virulent s. suis 2 strains, from which a novel genomic island (gei), associated with disease onset and progression and designated 89 k, was identified. here, an avirulent strain, 05has68, was isolated from a clinically healthy pig. | 2017 | 28292261 |
| critical streptococcus suis virulence factors: are they all really critical? | streptococcus suis is an important swine pathogen that can be transmitted to humans by contact with diseased animals or contaminated raw pork products. this pathogen possesses a coat of capsular polysaccharide (cps) that confers protection against the immune system. yet, the cps is not the only virulence factor enabling this bacterium to successfully colonize, invade, and disseminate in its host leading to severe systemic diseases such as meningitis and toxic shock-like syndrome. indeed, recent ... | 2017 | 28274524 |
| case report: two human streptococcus suis infections in borneo, sabah, malaysia. | streptococcus suis (s.suis) is increasingly being recognised as a potentially preventable emerging zoonotic infection in humans with a global distribution. it is a major cause of meningitis especially among those in contact with pigs and has also been associated with a toxic shock syndrome. | 2017 | 28257622 |
| capsular serotypes, virulence-associated genes and antimicrobial susceptibility of streptococcus suis isolates from pigs in korea. | streptococcus suis is an important pig pathogen with potential for human transmission. the serotype distributions and phenotypic characteristics vary over time and among regions; however, little is known about the characteristics of s. suis isolates in korea. in this study, 240 s. suis isolates collected from pigs in korea in 2009-2010 were serotyped by coagglutination tests, subsequently screened for three virulence-associated genes (mrp, epf and sly) and tested for antimicrobial susceptibility ... | 2017 | 28250312 |
| in vitro and in vivo antibacterial effect of nz2114 against streptococcus suis type 2 infection in mice peritonitis models. | nz2114 is a promising candidate for therapeutic application owing to potent activity to gram-positive bacterium such as streptococcus pneumoniae and staphylococcus aureus. this work is the first report to describe the in vitro and in vivo antibacterial characteristics of nz2114 against streptococcus suis. it exhibited strong antimicrobial activity against s. suis type 2 strains cvcc 606, cvcc 3309, and cvcc 3928 at a low minimal inhibitory concentration (mic) of 0.03-0.06 μm. the nz2114 killed o ... | 2017 | 28220445 |
| genotyping and investigating capsular polysaccharide synthesis gene loci of non-serotypeable streptococcus suis isolated from diseased pigs in canada. | streptococcus suis (s. suis) is an important swine pathogen and an emerging zoonotic agent. most clinical s. suis strains express capsular polysaccharides (cps), which can be typed by antisera using the coagglutination test. in this study, 79 s. suis strains recovered from diseased pigs in canada and which could not be typed using antisera were further characterized by capsular gene typing and sequencing. four patterns of cps locus were observed: (1) fifteen strains were grouped into previously ... | 2017 | 28219415 |
| transcriptomic analysis reveals selective metabolic adaptation of streptococcus suis to porcine blood and cerebrospinal fluid. | streptococcus suis is a zoonotic pathogen that can cause severe pathologies such as septicemia and meningitis in its natural porcine host as well as in humans. establishment of disease requires not only virulence of the infecting strain but also an appropriate metabolic activity of the pathogen in its host environment. however, it is yet largely unknown how the streptococcal metabolism adapts to the different host niches encountered during infection. our previous isotopologue profiling studies o ... | 2017 | 28212285 |
| syringa oblata lindl. aqueous extract is a potential biofilm inhibitor in s. suis. | streptococcus suis (s. suis) is a zoonotic pathogen that causes severe disease symptoms in pigs and humans. syringa oblata lindl. distributed in the middle latitudes of eurasia and north america were proved as the most development potential of chinese medicine. in this study, biofilm formation by s. suis decreased after growth with 1/2 mic, 1/4 mic, or 1/8 mic of syringa oblata lindl. aqueous extract and rutin. scanning electron microscopy analysis revealed the potential effect of syringa oblata ... | 2017 | 28194111 |
| lysine-tryptophan-crosslinked peptides produced by radical sam enzymes in pathogenic streptococci. | macrocycles represent a common structural framework in many naturally occurring peptides. several strategies exist for macrocyclization, and the enzymes that incorporate them are of great interest, as they enhance our repertoire for creating complex molecules. we recently discovered a new peptide cyclization reaction involving a crosslink between the side chains of lysine and tryptophan that is installed by a radical sam enzyme. herein, we characterize relatives of this metalloenzyme from the pa ... | 2017 | 28191919 |
| isolation and partial characterization of streptococcus suis from clinical cases in cattle. | sixteen isolates of gram-positive, coccoid bacteria were obtained from clinical cases of diverse conditions in cattle and identified as streptococcus suis using 16s ribosomal dna gene sequencing and other bacterial identification methods. none of the isolates could be assigned to any of the known s. suis capsular types. virulence-associated gene profiling that targeted muramidase-released protein, extracellular protein factor, suilysin, 89-kb pathogenicity island, and arginine deiminase ( arca) ... | 2017 | 28166707 |
| factor h specifically capture novel factor h-binding proteins of streptococcus suis and contribute to the virulence of the bacteria. | factor h (fh), a regulatory protein of the complement system, can bind specifically to factor h-binding proteins (fhbps) of streptococcus suis serotype 2 (ss2), which contribute to evasion of host innate immune defenses. in the present study, we aimed to identify novel fhbps and characterize the biological functions of fh in ss2 pathogenesis. here, a method that combined proteomics and far-western blotting was developed to identify the surface fhbps of ss2. with this method, fourteen potential n ... | 2017 | 28164787 |
| transcutaneous immunization of streptococcus suis bacterin using dissolving microneedles. | vaccine delivery using microneedle (mn) patches is an easy, safe and painless alternative to traditional needle injections. in this study, we examined whether mn patches can enhance the efficacy of a streptococcus suis serotype 2 (s. suis 2) vaccine in a mouse model. results showed that mns can reach 200-250μm into the skin, a depth beneficial for targeted delivery of antigens to antigen-presenting cells in the epidermis and dermis. vaccination with prime-boost of mn induced higher levels of igg ... | 2017 | 28131383 |
| streptococcus suis small rna rss04 contributes to the induction of meningitis by regulating capsule synthesis and by inducing biofilm formation in a mouse infection model. | streptococcus suis (ss) is an important pathogen for pigs, and it is also considered as a zoonotic agent for humans. meningitis is one of the most common features of the infection caused by ss, but little is known about the mechanisms of ss meningitis. recent studies have revealed that small rnas (srnas) have emerged as key regulators of the virulence in several bacteria. in the previous study, we reported that ss srna rss04 was up-regulated in pig cerebrospinal fluid and contributes to ss virul ... | 2017 | 28110777 |
| draft genome sequence of hypervirulent and vaccine candidate streptococcus suis strain sc19. | streptococcus suis, a zoonotic bacterium found primarily in pigs, has been recognized recently as an emerging pathogen of humans. herein, we describe the genome of streptococcus suis strain sc19, a hypervirulent and vaccine candidate strain isolated from a pig amid the 2005 outbreak in china. | 2017 | 28104658 |
| causes and outcomes of sepsis in southeast asia: a multinational multicentre cross-sectional study. | improved understanding of pathogens that cause sepsis would aid management and antimicrobial selection. in this study, we aimed to identify the causative pathogens of sepsis in southeast asia. | 2017 | 28104185 |
| the involvement of msmk in pathogenesis of the streptococcus suis serotype 2. | streptococcus suis serotype 2 (ss2) is an important swine and human pathogen that causes global economic and public health problems. virulent s. suis strains successfully maintain high bacterial concentrations in host blood and rapidly adapt to challenging environments within hosts. successful survival in hosts is a major factor influencing the pathogenesis of ss2. we have previously identified that ss2 colonization in mouse brain is possibly affected by the atpase, msmk of carbohydrate atp-bind ... | 2017 | 28102028 |
| serotypes of streptococcus suis isolated from healthy pigs in phayao province, thailand. | streptococcus suis (s. suis) is an important swine and human pathogen. there are 33 serotypes that have been described. zoonotic cases are very common the northern part of thailand, especially in phayao province. however, the prevalence of s. suis and, more particularly the different serotypes, in pigs in this region is poorly known and needed to be addressed. | 2017 | 28100261 |
| the 1910hk/rr two-component system is essential for the virulence of streptococcus suis serotype 2. | streptococcus suis serotype 2 is a major zoonotic pathogen, and the two-component system plays an important role in bacterial pathogenesis. the present study targeted the 1910hk/rr two-component system of s. suis 2. a 1910hk/rr deletion mutant (δ1910hk/rr) and the corresponding complementation strain (cδ1910hk/rr) were constructed in s. suis 2 strain 05zyh33. 1910hk/rr deletion had no effect on s. suis 2 growth, but significantly inhibited the adherence and invasion of s. suis 2 to hep-2 cells. ... | 2017 | 28093234 |
| pathogenic streptococcus strains employ novel escape strategy to inhibit bacteriostatic effect mediated by mammalian peptidoglycan recognition protein. | pathogenic streptococcal species are responsible for some of the most lethal and prevalent animal and human infections. previous reports have identified a candidate pathogenicity island (pai) in two highly virulent clinical isolates of streptococcus suis type 2, a causative agent of high-mortality streptococcal toxic shock syndrome. this pai contains a type-ivc secretion system c subgroup (type-ivc secretion system) that is involved in the secretion of unknown pathogenic effectors that are respo ... | 2017 | 28092693 |
| deletion of ssna attenuates the pathogenicity of streptococcus suis and confers protection against serovar 2 strain challenge. | streptococcus suis serotype 2 (ss2) is a major porcine and human pathogen which causes arthritis, meningitis, and septicemia. streptococcus suis nuclease a (ssna) is a recently discovered deoxyribonuclease (dnase), which has been demonstrated to contribute to escape killing in neutrophil extracellular traps (nets). to further determine the effects of ssna on virulence, the ssna deletion mutant (δssna) and its complemented strain (c-δssna) were constructed. the ability of δssna mutant to interact ... | 2017 | 28081204 |
| distribution of suicin gene clusters in streptococcus suis serotype 2 belonging to sequence types 25 and 28. | recently, we reported the purification and characterization of three distinct lantibiotics (named suicin 90-1330, suicin 3908, and suicin 65) produced by streptococcus suis. in this study, we investigated the distribution of the three suicin lantibiotic gene clusters among serotype 2 s. suis strains belonging to sequence type (st) 25 and st28, the two dominant sts identified in north america. the genomes of 102 strains were interrogated for the presence of suicin gene clusters encoding suicins 9 ... | 2016 | 28078298 |
| occurrence of pasteurella multocida among pigs with respiratory disease in china between 2011 and 2015. | prior to the 1990s, p. multocida capsular serogroup a was the most prevalent in china, followed by serogroups b and d. thirty years later, serogroup d became the most prevalent, followed by serogroups a and b. however, the p. multocida capsular serogroups currently circulating in china remain unclear. therefore, the aim of the present study was to provide an update on p. multocida serogroups isolated from diagnostic samples collected from clinically diseased pigs in central and eastern china fro ... | 2017 | 28078081 |
| temporal regulation of the transformasome and competence development in streptococcus suis. | in s. suis the comx-inducing peptide (xip) pheromone regulates comr-dependent transcriptional activation of comx (or sigx) the regulator of the late competence regulon. the aims of this study were to identify the comr-regulated genes and in s. suis using genome-wide transcriptomics and identify their function based on orthology and the construction of specific knockout mutants. the comx regulon we identified, includes all homologs of the "transformasome" a type 4-like pilus dna binding and trans ... | 2016 | 28066332 |
| immunization with streptococcus suis bacterin plus recombinant sao protein in sows conveys passive immunity to their piglets. | streptococcus suis (s. suis) causes arthritis, meningitis, septicemia, and sudden death in pigs and is also an zoonotic agent for humans. the present study demonstrated that immunization with recombinant sao-l (surface antigen one-l, rsao-l) protein from a strain of s. suis serotype 2 in pigs was able to increase cross-serotype protection against s. suis serotype 1 and 2 challenge. since weaning piglets are more susceptible to s. suis infections due to the stresses associated with weaning, prepa ... | 2017 | 28061775 |
| development of an indirect dot-ppa-elisa using glutamate dehydrogenase as a diagnostic antigen for the rapid and specific detection of streptococcus suis and its application to clinical specimens. | streptococcus suis is an important zoonotic pathogen causing infections in pigs and humans. bacterial surface-related proteins are often explored as potential vaccine candidates and diagnostic antigens. in the present study, glutamate dehydrogenase, a highly conserved immunogenic extracellular protein, was used to establish a dot horseradish peroxidase enzyme-linked staphylococcal protein a immunosorbent assay (dot-ppa-elisa) for diagnosis of s. suis infection. the antigen-antibody reaction was ... | 2017 | 28058577 |
| a serine/threonine phosphatase 1 of streptococcus suis type 2 is an important virulence factor. | streptococcus suis is regarded as one of the major pathogens of pigs while s. suis type 2 (ss2) is considered to be a zoonotic bacterium for its ability to cause meningitis and streptococcal toxic shock-like syndrome in humans. many bacterial species contain genes encoding serine/threonine protein phosphatases (stp) responsible for dephosphorylation of their substrates in a single reaction step. this study was aimed to investigate the role of stp1 in pathogenesis of ss2. an isogenic stp1 mutant ... | 2017 | 28057904 |
| the phage lysin plyss2 decolonizes streptococcus suis from murine intranasal mucosa. | streptococcus suis infects pigs worldwide and may be zoonotically transmitted to humans with a mortality rate of up to 20%. s. suis has been shown to develop in vitro resistance to the two leading drugs of choice, penicillin and gentamicin. because of this, we have pursued an alternative therapy to treat these pathogens using bacteriophage lysins. the bacteriophage lysin plyss2 is derived from an s. suis phage and displays potent lytic activity against most strains of that species including sero ... | 2017 | 28046082 |
| detection of multiple parallel transmission outbreak of streptococcus suis human infection by use of genome epidemiology, china, 2005. | streptococcus suis sequence type 7 emerged and caused 2 of the largest human infection outbreaks in china in 1998 and 2005. to determine the major risk factors and source of the infections, we analyzed whole genomes of 95 outbreak-associated isolates, identified 160 single nucleotide polymorphisms, and classified them into 6 clades. molecular clock analysis revealed that clade 1 (responsible for the 1998 outbreak) emerged in october 1997. clades 2-6 (responsible for the 2005 outbreak) emerged se ... | 2017 | 27997331 |
| roles of the putative type iv-like secretion system key component vird4 and prsa in pathogenesis of streptococcus suis type 2. | streptococcus suis type 2 (ss2) is a zoonotic pathogen causing septic infection, meningitis and pneumonia in pigs and humans. ss2 may cause streptococcal toxic shock syndrome (stss) probably due to excessive release of inflammatory cytokines. a previous study indicated that the vird4 gene in the putative type iv-like secretion system (t4ss) within the 89k pathogenicity island specific for recent epidemic strains contributed to the development of stss. however, the functional basis of vird4 in st ... | 2016 | 27995095 |
| evaluation of galleria mellonella larvae for studying the virulence of streptococcus suis. | streptococcus suis is an encapsulated gram-positive bacterium and the leading cause of sepsis and meningitis in young pigs, resulting in considerable economic losses in the porcine industry. s. suis is considered an emerging zoonotic agent with increasing numbers of human cases over the last years. in the environment, both avirulent and virulent strains occur in pigs, with no evidence for consistent adapatation of virulent strains to the human host. currently, there is an urgent need for a conve ... | 2016 | 27978817 |
| immune-responsiveness of cd4(+) t cells during streptococcus suis serotype 2 infection. | the pathogenesis of streptococcus suis infection, a major swine and human pathogen, is only partially understood and knowledge on the host adaptive immune response is critically scarce. yet, s. suis virulence factors, particularly its capsular polysaccharide (cps), enable this bacterium to modulate dendritic cell (dc) functions and potentially impair the immune response. this study aimed to evaluate modulation of t cell activation during s. suis infection and the role of dcs in this response. s. ... | 2016 | 27905502 |
| distribution of type i restriction-modification systems in streptococcus suis: an outlook. | streptococcus suis is a porcine commensal and pathogen with zoonotic potential. we recently identified a novel type i restriction-modification (r-m) system in a zoonotic s. suis clone which has emerged in the netherlands. here, we describe the dna inversions in the specificity subunit of this system in s. suis serotype 2, clonal complex 20 and explain the absence of domain movement by the absence of repeats. in addition, we identified a core type i r-m system present in 95% of the isolates and f ... | 2016 | 27869755 |
| mac protein is not an essential virulence factor for the virulent reference strain streptococcus suis p1/7. | streptococcus suis is a major pathogen of pigs and also an important zoonotic agent for humans. a s. suis protein containing mac-1 domain (designated mac) is a protective antigen, exclusively cleaves porcine igm, and contributes to complement evasion with the presence of high titers of specific porcine anti-s. suis igm, but its role in s. suis virulence has not been investigated in natural healthy host without specific igm. in this study, a mac deletion mutant was constructed by homologous recom ... | 2017 | 27847975 |
| structural and functional analysis of an anchorless fibronectin-binding protein fbps from gram-positive bacterium streptococcus suis. | the anchorless fibronectin-binding proteins (fnbps) are a group of important virulence factors for which the structures are not available and the functions are not well defined. in this study we performed comprehensive studies on a prototypic member of this group: the fibronectin-/fibrinogen-binding protein from streptococcus suis (fbps). the structures of the n- and c-terminal halves (fbps-n and fbps-c), which together cover the full-length protein in sequence, were solved at a resolution of 2. ... | 2016 | 27834729 |
| suilysin-induced platelet-neutrophil complexes formation is triggered by pore formation-dependent calcium influx. | platelet activation and platelet-neutrophil interactions have been found to be involved in inflammation, organ failure and soft-tissue necrosis in bacterial infections. streptococcus suis, an emerging human pathogen, can cause streptococcal toxic-shock syndrome (stss) similarly to streptococcus pyogenes. currently, s. suis-platelet interactions are poorly understood. here, we found that suilysin (sly), the s. suis cholesterol-dependent cytolysin (cdc), was the sole stimulus of s. suis that induc ... | 2016 | 27830834 |
| human meningitis due to streptococcus suis in lomé, togo: a case report. | streptococcus suis is a zoonotic pathogen which represents the leading cause of meningitis in southeast asia and an emerging pathogen in the western world, the main risk factor for infection being contact with pigs. in africa, the prevalence of s. suis infections in swine and humans is largely unrecognized, with only one recent report of a limited case series. | 2016 | 27821092 |
| acute meningitis of piglets and mice caused by co-infected with streptococcus suis and aerococcus viridans. | the two opportunistic pathogens, streptococcus suis (s. suis) and aerococcus. viridans (a. viridans) were isolated from the brains of piglets suffered bacterial meningitis in a farm of china. the murine model has been established to evaluate the pathogenicity and symbiotic relationship of s. suis and a. viridans simultaneously infection. our results demonstrated the ability of new serotype s. suis to cause the classical bacterial meningitis and death were greatly enhanced during co-infection wit ... | 2016 | 27816682 |
| sub-mics of azithromycin decrease biofilm formation of streptococcus suis and increase capsular polysaccharide content of s. suis. | streptococcus suis (s. suis) caused serious disease symptoms in humans and pigs. s. suis is able to form thick biofilms and this increases the difficulty of treatment. after growth with 1/2 minimal inhibitory concentration (mic) of azithromycin, 1/4 mic of azithromycin, or 1/8 mic of azithromycin, biofilm formation of s. suis dose-dependently decreased in the present study. furthermore, scanning electron microscopy analysis revealed the obvious effect of azithromycin against biofilm formation of ... | 2016 | 27812354 |
| effects of suilysin on streptococcus suis-induced platelet aggregation. | blood platelets play important roles during pathological thrombocytopenia in streptococcal toxic shock syndrome (stss). streptococcus suis (s. suis) an emerging human pathogen, can cause stss similarly to s. pyogenes. however, s. suis interactions with platelets are poorly understood. here, we found that suilysin (sly), different from other bacterial cholesterol-dependent cytolysins (cdcs), was the sole stimulus that induced platelet aggregation. furthermore, the inside-out activation of gpiib/i ... | 2016 | 27800304 |
| a decennium of etiology and antimicrobial susceptibility patterns in patients with infective endocarditis at a university hospital, thailand. | infective endocarditis is an infection with a high mortality rate. antimicrobial therapy is important in treatment but data on antimicrobial susceptibilities are limited. this retrospective study analyzed the data of causative microorganisms and antimicrobial susceptibility patterns in patients with infective endocarditis who were aged 18 years or older and received inpatient care between 2006 and 2015 at king chulalongkorn memorial hospital. a total of 213 patients fulfilled the inclusion crite ... | 2016 | 27795476 |
| evolution and diversity of the antimicrobial resistance associated mobilome in streptococcus suis: a probable mobile genetic elements reservoir for other streptococci. | streptococcus suis is a previously neglected, newly emerging multidrug-resistant zoonotic pathogen. mobile genetic elements (mges) play a key role in intra- and interspecies horizontal transfer of antimicrobial resistance (amr) determinants. although, previous studies showed the presence of several mges, a comprehensive analysis of amr-associated mobilome as well as their interaction and evolution has not been performed. in this study, we presented the amr-associated mobilome and their insertion ... | 2016 | 27774436 |
| egfr transactivation contributes to neuroinflammation in streptococcus suis meningitis. | streptococcus suis serotype 2 (ss2) is an important zoonotic bacterial pathogen in both humans and animals, which can cause high morbidity and mortality. meningitis is one of the major clinical manifestations of ss2 infection. however, the specific process of ss2 meningitis and its molecular mechanisms remain unclear. epidermal growth factor receptor (egfr) has been reported to initiate transduction of intracellular signals and regulate host inflammatory responses. whether and how egfr contribut ... | 2016 | 27756321 |
| use of antibiotics and antimicrobial resistance in veterinary medicine as exemplified by the swine pathogen streptococcus suis. | use of antimicrobial agents in veterinary medicine is essential to control infectious diseases, thereby keeping animals healthy and animal products safe for the consumer. on the other hand, development and spread of antimicrobial resistance is of major concern for public health. streptococcus (s.) suis reflects a typical bacterial pathogen in modern swine production due to its facultative pathogenic nature and wide spread in the pig population. thus, in the present review we focus on certain cur ... | 2016 | 27738916 |
| serological patterns of actinobacillus pleuropneumoniae, mycoplasma hyopneumoniae, pasteurella multocida and streptococcus suis in pig herds affected by pleuritis. | respiratory illness is traditionally regarded as the disease of the growing pig, and has historically mainly been associated to bacterial infections with focus on mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae. these bacteria still are of great importance, but continuously increasing herd sizes have complicated the scenario and the influence of secondary invaders may have been increased. the aim of this study was to evaluate the presence of a. pleuropneumoniae and m. hyopneumoniae, ... | 2016 | 27716292 |
| survival of streptococcus suis, streptococcus dysgalactiae and trueperella pyogenes in dry-cured iberian pork shoulders and loins. | dry-cured hams, shoulders and loins of iberian pigs are highly appreciated in national and international markets. salting, additive addition and dehydration are the main strategies to produce these ready-to-eat products. although the dry curing process is known to reduce the load of well-known food borne pathogens, studies evaluating the viability of other microorganisms in contaminated pork have not been performed. in this work, the efficacy of the dry curing process to eliminate three swine pa ... | 2017 | 27697171 |
| novel capsular polysaccharide loci and new diagnostic tools for high-throughput capsular gene typing in streptococcus suis. | streptococcus suis is an important pathogen of pigs and may cause serious disease in humans. serotyping is an important tool for detection and epidemiological studies of s. suis thirty-three reference serotypes and nine novel cps loci (ncls) are recognized in s. suis to gain a better understanding of the prevalence and genetic characteristics of ncls, we investigated the serotype identity of 486 isolates isolated between 2013 and 2015 in china by capsular gene typing methods. two hundred seventy ... | 2016 | 27694240 |
| predominance of streptococcus suis st1 and st7 in human cases in china, and detection of a novel sequence type, st658. | 2016 | 27689249 | |
| human case of bacteremia due to streptococcus suis serotype 5 in japan: the first report and literature review. | streptococcus suis is a zoonotic pathogen that can be transferred from pigs to humans. the serotypes 2 and 14 are prevalent among patients with s. suis infections, while other serotypes (i.e., 1, 4, 5, 16, and 24) have been detected in rare human cases. to the best of our knowledge, the present patient handling with raw pork is the first human case of uncomplicated bacteremia due to s. suis serotype 5 in japan. we confirmed the new sequence type 752 of this isolate. virulence-associated gene pro ... | 2016 | 27689023 |
| selection of potential virulence factors contributing to streptococcus suis type 2 penetration into the blood brain barrier in an in vitro co-culture model. | meningitis caused by streptococcus suis type 2 (s. suis 2) is a great threat to the pig industry and human health. virulence factors associated with the pathogenesis of meningitis have yet to be clearly defined, even though many potential s. suis 2 virulence factors have been identified. this greatly hinders the progress of s. suis 2 meningitis pathogenesis research. in this study, a co-culture blood brain barrier (bbb) model was established using primary porcine brain microvascular endothelial ... | 2016 | 27666982 |
| streptococcus suis serotype 2 capsule in vivo. | many streptococcus suis isolates from porcine endocarditis in slaughterhouses have lost their capsule and are considered avirulent. however, we retrieved capsule- and virulence-recovered s. suis after in vivo passages of a nonencapsulated strain in mice, suggesting that nonencapsulated s. suis are still potentially hazardous for persons in the swine industry. | 2016 | 27648583 |
| suilysin stimulates the release of heparin binding protein from neutrophils and increases vascular permeability in mice. | most of the deaths that occurred during two large outbreaks of streptococcus suis infections in 1998 and 2005 in china were caused by streptococcal toxic shock syndrome (stss), which is characterized by increased vascular permeability. heparin-binding protein (hbp) is thought to mediate the vascular leakage. the purpose of this study was to investigate the detailed mechanism underlying the release of hbp and the vascular leakage induced by s. suis. significantly higher serum levels of hbp were d ... | 2016 | 27617009 |
| development of a sandwich elisa for ehec o157:h7 intimin γ1. | enterohemorrhagic escherichia coli (ehec) o157:h7 is a zoonotic pathogen of worldwide importance that causes foodborne infections in humans. intimin gamma 1 (intimin γ1) is one of the most important outer membrane proteins required for ehec's intimate adhesion to epithelial cells. here, we generated a polyclonal antibody (pab) and a monoclonal antibody (mab) against intimin γ1 to develop a double antibody sandwich elisa (das-elisa) with increased sensitivity and specificity for measuring ehec o1 ... | 2016 | 27603508 |
| a survey of porcine respiratory disease complex (prdc) associated pathogens among commercial pig farms of korea via oral fluid method. | oral fluid analysis for monitoring herd is an interest in commercial pig farms in korea. the aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (prdc) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. the samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (rt-pcr) or standard pcr depending on microorganisms. the pathogens ... | 2016 | 27586468 |
| a fatal case of streptococcal toxic shock syndrome caused by streptococcus suis carrying tet (40) and tet (o/w/32/o), italy. | we report the first human fatal case of streptococcal toxic shock syndrome (stss) caused by streptococcus suis serotype 2 carrying the tetracycline efflux tet (40) gene and the tetracycline ribosomal protection tet (o/w/32/o) gene. the patient was splenectomized. the case was characterized by multi-organ dysfunction and disseminated intravascular coagulopathy, in accordance with the clinical parameters of stss. more investigations are needed to improve the epidemiology and the pathogenesis of s. ... | 2016 | 27553071 |
| genomic and proteomic characterization of se-i, a temperate bacteriophage infecting erysipelothrix rhusiopathiae. | a bacteriophage infecting pathogenic erysipelothrix rhusiopathiae was isolated from a swine farm experiencing an outbreak of acute swine erysipelas; we designated this phage se-i. se-i has an icosahedral head, a long tail and a double-stranded dna genome. the 34,997-bp genome has a gc content of 34 % and contains 43 open reading frames (orfs) encoding packaging, structural, lysin-holin, and hypothetical proteins. components of purified se-i were separated using sds-page and analyzed using liquid ... | 2016 | 27541818 |
| initial steps of the pathogenesis of the infection caused by streptococcus suis: fighting against nonspecific defenses. | interactions between a bacterial pathogen and its potentially susceptible host are initiated with the colonization step. during respiratory/oral infection, the pathogens must compete with the normal microflora, resist defense mechanisms of the local mucosal immunity, and finally reach, adhere, and breach the mucosal epithelial cell barrier in order to induce invasive disease. this is the case during infection by the swine and zoonotic pathogen streptococcus suis, which is able to counteract muco ... | 2016 | 27539145 |
| characterisation of a novel integrative and conjugative element icessd9 carrying erm(b) and tet(o) resistance determinants in streptococcus suis, and the distribution of icessd9-like elements in clinical isolates. | this study identified a novel integrative and conjugative element (icessd9) carrying erm(b) and tet(o) resistance determinants in streptococcus suis d9 and determined its prevalence in clinical isolates. comparative genome analysis was performed using mauve and artemis comparison tool visualisation programs. inverse pcr was utilised to detect its circular intermediate. the transfer capacity of icessd9 was evaluated by mating assays using s. suis a7 and enterococcus faecalis jh2-2 as recipients. ... | 2016 | 27531000 |