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benefits of bifidobacterium animalis subsp. lactis probiotic in experimental periodontitis.this study evaluates effects of topical administration of probiotic bacteria of the genus bifidobacterium on experimental periodontitis (ep) in rats.201727660886
metabolomics evaluation of the impact of smokeless tobacco exposure on the oral bacterium capnocytophaga sputigena.the association between exposure to smokeless tobacco products (stp) and oral diseases is partially due to the physiological and pathological changes in the composition of the oral microbiome and its metabolic profile. however, it is not clear how stps affect the physiology and ecology of oral microbiota. a uplc/qtof-ms-based metabolomics study was employed to analyze metabolic alterations in oral bacterium, capnocytophaga sputigena as a result of smokeless tobacco exposure and to assess the cap ...201627480511
quantitative molecular detection of 19 major pathogens in the interdental biofilm of periodontally healthy young adults.in oral health, the interdental spaces are a real ecological niche for which the body has few or no alternative defenses and where the traditional daily methods for control by disrupting biofilm are not adequate. the interdental spaces are the source of many hypotheses regarding their potential associations with and/or causes of cardiovascular disease, diabetes, chronic kidney disease, degenerative disease, and depression. this pcr study is the first to describe the interdental microbiota in hea ...201627313576
influence of the periodontal status on the initial-biofilm formation on titanium surfaces.dental implants will be exposed to a complex ecosystem once they are placed in the oral cavity. the bacterial colonization and biofilm formation on these devices will depend not only on the physicochemical surface implant properties but also on the periodontal health conditions of the patients, as these devices are exposed.201624995517
capnocytophaga sputigena bacteremia in a patient with chronic lymphocytic leukemia. 201424982840
microbial diversity in persistent root canal infections investigated by checkerboard dna-dna hybridization.the aim of the present study was to investigate the composition of the root canal microbiota in endodontic failures in order to identify and quantify these microorganisms.201424935532
16s rdna-based metagenomic analysis of dental plaque and lung bacteria in patients with severe acute exacerbations of chronic obstructive pulmonary disease.acute exacerbations of chronic obstructive pulmonary disease (ae-copd) are leading causes of mortality in hospital intensive care units. we sought to determine whether dental plaque biofilms might harbor pathogenic bacteria that can eventually cause lung infections in patients with severe ae-copd.201424484531
phototargeting human periodontal pathogens in vivo.the effects of blue light at 455 nm were investigated on the bacterial composition of human dental plaque in vivo. eleven subjects who refrained from brushing for 3 days before and during phototherapy participated in the study. light with a power density of 70 mw/cm(2) was applied to the buccal surfaces of premolar and molar teeth on one side of the mouth twice daily for 2 min over a period of 4 days. dental plaque was harvested at baseline and again at the end of 4 days from eight posterior tee ...201524346334
high prevalence of β-lactam and macrolide resistance genes in human oral capnocytophaga species.to determine macrolide-lincosamide-streptogramin (mls) resistance determinants in the capnocytophaga genus and to describe the prevalence of β-lactam resistance genes in human oral capnocytophaga species.201424013195
the cultivable human oral gluten-degrading microbiome and its potential implications in coeliac disease and gluten sensitivity.coeliac disease is characterized by intestinal inflammation caused by gluten, proteins which are widely contained in the western diet. mammalian digestive enzymes are only partly capable of cleaving gluten, and fragments remain that induce toxic responses in patients with coeliac disease. we found that the oral microbiome is a novel and rich source of gluten-degrading organisms. here we report on the isolation and characterization of the cultivable resident oral microbes that are capable of clea ...201323714165
capnocytophaga sputigena empyema.empyema is a problem faced by clinicians worldwide. most cases are caused by gram-positive organisms, such as streptococci and staphylococci. empyema caused by capnocytophaga spp. is extremely uncommon (h. bonatti et al., clin. microbiol. infect. 9:380-387, 2003). we present a unique case of a polymicrobial empyema, with a predominance of capnocytophaga sputigena, in an older patient with no history of animal bite or splenectomy.201323698526
[fatal bacteremia related to capnocytophaga sputigena in a hematological patient with type t non-hodgkin lymphoma. diagnosis by 16s rrna gene sequencing].we described a case of fatal bacteremia related to capnocytophaga sputigena in a hematological patient. the strain was identified by 16s rrna gene sequencing.201523102464
capnocytophaga species and perinatal infections: case report and review of the literature.capnocytophaga species are part of the normal human oral bacterial flora.they are recognized as opportunistic pathogens leading to various extra-oral infections including septicemia, osteomyelitis, abscesses and keratitis and they have been rarely reported as a cause of chorioamionitis and neonatal infection. we here report the first two cases of chorioamionitis produced by capnocytophaga sputigena and the recently described c. leadbetteri in belgium. both isolates were correctly identified at t ...201622480039
distribution of periodontopathic bacterial species in dogs and their owners.presently, a large number of individuals consider their companion animals as family members and have close contact with them in daily life. the purpose of the present study was to analyze the distribution of periodontopathic bacterial species in oral specimens taken from dogs and their owners.201222417880
impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray.this study compares the changes to the subgingival microbiota of individuals with "refractory" periodontitis (rp) or treatable periodontitis (good responders [gr]) before and after periodontal therapy by using the human oral microbe identification microarray (homim) analysis.201222324467
investigation of six selected bacterial species in endo-periodontal lesions.didilescu ac, rusu d, anghel a, nica l, iliescu a, greabu m, bancescu g, stratul si. investigation of six selected bacterial species in endo-periodontal lesions. international endodontic journal, 45, 282-293, 2012. abstract: aim  to investigate and determine possible associations of six tested bacteria belonging to 'orange' and 'green' complexes, in endo-periodontal lesions: parvimonas micra, fusobacterium nucleatum, campylobacter rectus, eubacterium nodatum, eikenella corrodens and capnocytopha ...201122077868
molecular detection of human periodontal pathogens in oral swab specimens from dogs in japan.periodontal diseases are known to be major diseases in humans, and are also common in dogs. the purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. the distribution of an animal gingival organism porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including porphyromonas gingivalis, treponema denticola, tannerella forsythia, capnocytophaga ochracea, capnocyt ...201121916371
Early microbial succession in redeveloping dental biofilms in periodontal health and disease.Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ...201121895662
evaluation of the subgingival microbiota of alcoholic and non-alcoholic individuals.the aim of this study was to evaluate the composition of the subgingival microbiota of alcoholic and non-alcoholic individuals.201121864643
il6 -174 genotype associated with aggregatibacter actinomycetemcomitans in indians.genetic factors have recently been associated with presence of aggregatibacter actinomycetemcomitans subgingivally in populations living in industrialized countries. the aim of this study was to analyse associations between interleukin-6 (il6) single nucleotide polymorphisms and presence and levels of a. actinomycetemcomitans and other subgingival microbes in a rural indian population.201120860759
capnocytophaga sputigena primary iliopsoas abscess.iliopsoas abscess is usually secondary to the spread of infection from a contiguous focus. primary disease is uncommon, except in children where staphylococcus aureus is the main pathogen. we report a 60-year-old woman who developed a primary iliopsoas abscess as a result of haematogenous spread of capnocytophaga sputigena from a palatal fistula and chronic sinusitis due to previous treatment for nasopharyngeal carcinoma. pyomyositis due to unusual and fastidious gram-negative bacilli should be ...201020634330
characterization of csp-1, a novel extended-spectrum beta-lactamase produced by a clinical isolate of capnocytophaga sputigena.the capnocytophaga sputigena isolate nor, responsible for septicemia, was resistant to amoxicillin and narrow-spectrum cephalosporins. in a cloning experiment, a new gene, bla(csp-1), was identified; this gene encodes a novel extended-spectrum beta-lactamase (esbl) that shares only 52% and 49% identities with the cme-1 and veb-1 beta-lactamases, respectively. the g+c content of this gene, its genetic environment, the absence of conjugation transfer, and its detection in two reference strains sug ...201020308380
distribution of periodontopathic bacterial species in japanese children with developmental disabilities.recent developments in molecular biological techniques have enabled rapid detection of periodontopathic bacterial species in clinical specimens. accumulated evidence suggests that detection of specific bacterial species enables identification of subjects at high risk for the onset of periodontitis. we investigated the distribution of 10 selected periodontopathic bacterial species in dental plaque specimens obtained from children with disabilities who were attending daycare centers.200919772671
comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray.this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim).200919722792
does pregnancy have an impact on the subgingival microbiota?we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care.200919228092
microbiological findings in subjects with asymptomatic oral lichen planus: a cross-sectional comparative study.the bacterial colonization of the oral mucosa was evaluated in patients with asymptomatic oral lichen planus (olp) and compared to the microbiologic status in mucosally healthy subjects.200819053926
[capnocytophaga sputigena pleuropneumonitis: a case report].community-acquired pneumonia is complicated by purulent pleural effusion in approximately 28% of affected children. its incidence has increased over the last decade. streptococcus pneumoniae is the most frequent causal microorganism, encountered in roughly 80% of cases. we report the case of an immunocompetent 13-year-old teenager wearing orthodontic braces who suffered from pleuropneumonia due to capnocytophaga sputigena. to date, this gram-negative bacillus, a commensal of the buccal cavity, h ...200818804973
first case of bacteremia due to chromosome-encoded cfxa3-beta-lactamase-producing capnocytophaga sputigena in a pediatric patient with acute erythroblastic leukemia.bacteremia due to capnocytophaga sputigena occurred in a 4-year and 9-month-old japanese girl patient with acute erythroblastic leukemia in shinshu university hospital, japan. on her admission to the hospital, she had a temperature of 38.2 degrees c with canker sore. prior to the commencement of chemotherapy, peripheral blood culture was carried out with the bact/alert 3d system ver. 4.00d (biomerieux japan ltd., tokyo, japan) using both the pf and the sn bottles. at 48 hrs of incubation, the sy ...200818499560
microbiological evaluation of primary endodontic infections in teeth with and without sinus tract.to examine the microbiological status of primary endodontic infections in teeth with and without a sinus tract.200818422583
[septicaemia with capnocytophaga sputigena in a newborn child].we describe in this article a case of septicaemia with capnocytophaga suptigena in a premature newborn child. the newborn child exhibited fever at birth, a light inflammatory syndrome and a respiratory failure. the germ was initially identified in the blood-culture of the newborn child by conventional techniques, and then confirmed by sequencing of the arn 16s. after investigation, it was also found in the mother's vaginal sample. capnocytophaga sputigena carried by the mother is certainly respo ...200818390433
clinical and microbiological analysis of subjects treated with brånemark or astratech implants: a 7-year follow-up study.to assess the impact of different implant systems on the clinical conditions and the microbiota at implants, and whether the presence of bacteria at tooth sites was predictive of the presence at implant sites.200818261121
diversity of capnocytophaga species in children and description of capnocytophaga leadbetteri sp. nov. and capnocytophaga genospecies ahn8471.bacteria of the genus capnocytophaga form part of the resident oral flora in children and adults. they are recognized as opportunistic pathogens of various extra-oral infections. the significance of individual species in periodontal and extra-oral diseases is unclear, due to the inability of conventional phenotypic tests to identify clinical isolates to species level. aiming at a clear distinction between species, we undertook a phylogenetic study of a collection of 102 capnocytophaga strains in ...200818218926
infection at titanium implants with or without a clinical diagnosis of inflammation.to assess the microbiota at implants diagnosed with peri-implantitis, implant mucositis, or being clinically healthy.200717517058
distribution of 10 periodontal bacteria in saliva samples from japanese children and their mothers.we analyzed the distribution of 10 periodontal bacteria species (porphyromonas gingivalis, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, campylobacter rectus, eikenella corrodens, actinobacillus actinomycetemcomitans, capnocytophaga ochracea, capnocytophaga sputigena, and treponema denticola) in children, and then compared their distribution in those children and their mothers, with special attention given to three of the species known as the red complex (p. gingivalis, ...200616269128
chromosome- and plasmid-encoded beta-lactamases in capnocytophaga spp.chromosome- and plasmid-encoded cfxa2 and cfxa3 beta-lactamases were detected in capnocytophaga spp. from oral sources in france, norway, and the united states. unidentified chromosome-encoded beta-lactamases were present in capnocytophaga sputigena. nucleotide sequence analysis of the cfxa3-encoding plasmid from c. ochracea revealed an unreported insertion sequence (iscoc1) upstream of the cfxa gene.200516127077
molecular identification of an invasive gingival bacterial community.a woman with neutropenia developed gingival hyperplasia. biopsy showed invasion of gingival tissue with mats of filamentous organisms, and molecular analysis by polymerase chain reaction and fluorescence in situ hybridization revealed capnocytophaga sputigena, leptotrichia species, and fusobacterium nucleatum. oral bacterial flora may cause invasive gingival disease with hyperplasia in immunocompromised patients.200515937752
rate of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis.although microbiological studies have identified more than 400 bacterial species in periodontal pockets, only a limited number have been implicated as periodontal pathogens. the purpose of this study was to investigate the incidence of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. bacterial samples were collected with sterile paper points from the deepest periodontal pockets ((5 mm) of 203 patients: 92 males and 111 females, aged 35-55 years. the samples were cul ...200415508748
mixed bacteremia with capnocytophaga sputigena and escherichia coli following bone marrow transplantation: case report and review. 200414712364
[in vitro sensitivity of oral gram-negative bacteria to the bactericidal activity of defensins].oral gram-negative bacteria, including actinobacillus actinomycetemcomitans, porphyromonas gingivalis, capnocytophaga sputigena, provotella intermedius, provotella melaninogenicus, have been associated with destructive periodontal infection. an ultrasensitive antibacterial assay was applied to determine the sensitivity of a battery of oral gram-negative bacteria to the bactericidal effects of the isolated human neutrophil defensins (hnp) and two kinds of rabbit defensins np1 and np2. all species ...199812078176
periodontopathic bacterial infection in childhood.little information is available on periodontopathic bacterial infection in childhood. we assessed the prevalence by age of 10 putative periodontopathic microorganisms in periodontally healthy children using a polymerase chain reaction (pcr) assay.200211846195
[utility of prolonged incubation and terminal subcultures of blood cultures from immunocompromised patients].the value of blind terminal subcultures (7 and 30 days) and prolonged incubation (30 days) of blood cultures from immunosuppressed patients was analyzed in the fundación favaloro, the fundación para la lucha contra las enfermedades neurológicas de la infancia and the hospital de niños ricardo gutiérrez. a total of 2707 blood cultures and 369 patients were included (transplantation of solid organs 154, oncohematologic disorders 106 and solid tumors 109). bact-alert bottles were incubated at 35 de ...200111594009
relationship of periodontopathic bacteria with early-onset periodontitis in down's syndrome.down's syndrome (ds) patients often develop severe early-onset marginal periodontitis in early adulthood; however, there is little information available on the microbiology of ds periodontitis.200111327065
pcr detection of capnocytophaga species in dental plaque samples from children aged 2 to 12 years.the purpose of this study was to detect the presence of capnocytophaga sputigena, c. ochracea, and c. gingivalis in plaque samples from the toothbrushes of 122 children, using a polymerase chain reaction (pcr) method. the subjects were 25, 85, and 12 children with healthy gingiva, gingivitis, and periodontitis, respectively, ranging in age from 2-12 years old. plaque samples were collected from all erupted tooth sites using a sterile toothbrush. the mean amount of dna recovered from the samples ...200111270602
microbial composition of supra- and subgingival plaque in subjects with adult periodontitis.the purpose of the present study was to compare and relate the microbial composition of supra and subgingival plaque in 23 adult periodontitis subjects (mean age 51 +/- 14 years).200011034118
early microbial colonization of permucosal implants in edentulous patients.in edentulous patients, the microbial colonization of permucosal implants of sintered hydroxyapatite was studied. samples were taken from mucosa and dentures before insertion of implants and from supra- and subgingival sites two to 10 weeks after insertion. in total, five patients and 10 implants with clinically healthy peri-implant tissues were studied. the samples were investigated by dark-field microscopy and anaerobic culture. the supragingival plaque of the implants was dominated by gram-po ...198710872401
characterization and cross-reactivity of rabbit antisera to plaque toxins.the effects of heat labile, high molecular weight water-soluble toxins from bacterial plaque on hl60 promyelocytic cells were examined. on gel filtration, four inhibitors of hl60 cell growth and two inhibitors of hela cell growth (pt1, pt2) were detected. the first and third hl60 cell inhibitors corresponded to the two hela cell inhibitors. the last eluted hl60 cell inhibitor (plaque leukotoxin, pl) did not inhibit hela cell growth. anti-pt2 antibodies reduced the activity of enriched pt2 by 20- ...198710870474
microbiology of subgingival plaque from children with localized prepubertal periodontitis.localized prepubertal periodontitis has been described as a host-defect mediated form of bacterially induced periodontitis, with an early onset and rapid progression around a few teeth in children prior to puberty. to further our understanding of the etiology of this disease, we have examined the microbiological components of subgingival dental plaque in 9 children with localized prepubertal periodontitis to determine if patterns of putative pathogens existed, and have compared these results wit ...198710870471
second-trimester abortion caused by capnocytophaga sputigena: case report.intra-amniotic infection is often the cause of a second-trimester abortion. the bacterial species involved include bacteria with low pathogenicity like ureaplasma urealyticum and various mycoplasma species. in this case we describe an intra-amniotic infection caused by capnocytophaga sputigena, often found in the normal bacterial flora of the oral cavity, but not in the vagina. oral sex during pregnancy was the most probable source of the infection. the aborted fetus showed signs of pneumonia up ...199910458530
chorioamnionitis with intact membranes caused by capnocytophaga sputigena.we report a case of chorioamnionitis with intact membranes caused by capnocytophaga sputigena. the pregnant woman was hospitalised in preterm labor without fever, neither tenderness, just regular contractions. in spite of the tocolitic treatment the patient gave birth to a girl at 29 weeks' gestation, weighing 1220 g and transferred to intensive care. the newborn had clinical and biological signs of infections and was initially treated by ampicillin, cephalosporin and metronidazol. capnocytophag ...199910221619
detection of dextranase-producing gram-negative oral bacteria.thirty-one strains of 23 gram-negative oral bacterial species were examined for dextran-degrading activity on agar plates containing blue dextran. one strain each of capnocytophaga ochracea, capnocytophaga sputigena, prevotella loescheii, prevotella melaninogenica and prevotella oralis had detectable dextranase activity. the culture supernatants of p. melaninogenica and p. oralis cells contained dextranases of multiple sizes, but those of the other three species had a single size of enzyme. a 56 ...19989872116
subgingival microflora and treatment in prepubertal periodontitis associated with chronic idiopathic neutropenia.prepubertal periodontitis affects both primary and permanent dentition. the purpose of this study was to examine the composition of subgingival microflora of the permanent dentition in an 11-year-old caucasian female, who had premature exfoliation of her deciduous teeth on her 5th year of age, and the response of this condition to the antibiotic therapy and supportive periodontal care. gingival tissues were highly inflamed and alveolar bone loss was detected radiographically. the girl had experi ...19989763332
subgingival microflora associated with nifedipine-induced gingival overgrowth.the purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (go). age was inversely associated with the go. plaque index and bleeding index showed a significant association with go, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of go. the gingival inflammation as e ...19989660335
subgingival distribution of periodontopathic bacteria in adult periodontitis and their susceptibility to minocycline-hcl.the purpose of this study was to investigate the distribution of several periodontopathic bacteria in adult periodontitis, their in vitro susceptibility to minocycline-hcl, and whether the efficacy of the drug changes with a decrease in bacterial susceptibility. twenty-one patients (43 to 75 years old) with 62 periodontal lesions from pockets > or =4 mm participated in the study. after subgingival sampling, an ointment containing 2% minocycline-hcl was applied locally to the selected pockets onc ...19989527568
characterization of protease activities in capnocytophaga spp., porphyromonas gingivalis, prevotella spp., treponema denticola and actinobacillus actinomycetemcomitans.protease activities in cell sonicates of defined bacterial strains were examined using peptide substrates and class-specific inhibitors. capnocytophaga spp. all produced serine dipeptidyl peptidase activity and arginine/lysine, elastase- and chymotrypsin-like enzymes with some metalloprotease characteristics. the elastase-like activity was strongest in capnocytophaga sputigena, but the others were greatest in capnocytophaga gingivalis. the latter also had a separate arginine-specific enzyme whic ...19979467394
hla-d types and serum igg responses to capnocytophaga in diabetes and periodontitis.serum igg responses to the cell envelope proteins (ceps) from capnocytophaga sputigena, capnocytophaga ochracea, and capnocytophaga gingivalis were examined in periodontally healthy and periodontitis subjects, both with and without type 1 diabetes (n = 60). serum igg responses to ceps were determined by immunoblotting with biotin-goat anti-human igg and an alkaline phosphatase-streptavidin system. reactivity was analyzed by transmission densitometry, digitization, and computer manipulation. the ...19979390475
myeloperoxidase isoform activities released by human neutrophils in response to dental and periodontal bacteria.myeloperoxidase is an enzyme released by polymorphonuclear leukocytes which has been used, experimentally, as an indicator of periodontal disease activity when measured in gingival crevicular fluid. there are three myeloperoxidase isoforms: mpo i, mpo ii and mpo iii. we examined the activities of myeloperoxidase isoforms released by neutrophils in response to serum-opsonized actinobacillus actinomycetemcomitans, haemophilus aphrophilus, eikenella corrodens, capnocytophaga sputigena and streptoco ...19979151641
novel fnr homologues identified in four representative oral facultative anaerobes: capnocytophaga ochracea, capnocytophaga sputigena, haemophilus aphrophilus, and actinobacillus actinomycetemcomitans.based upon dna sequence data and positive immunochemical reactivity of expressed protein, novel homologues of the fnr family were identified in four representative oral facultative anaerobes: capnocytophaga ochracea, capnocytophaga sputigena, haemophilus aphrophilus, and actinobacillus actinomycetemcomitans. the similarity to e. coli fnr and to hlyx (itself 71% similar to e. coli fnr, while regulating expression of hemolysin operon in actinobacillus pleuropneumoniae) was estimated from the deduc ...19968998988
degradation of lactoferrin by periodontitis-associated bacteria.the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ...19968961558
[subgingival distribution of periodontopathic bacteria in periodontic patients and susceptibility of these bacteria to minocycline-hcl].the present study was carried out to examine the distribution of six periodontopathic bacteria in deep periodontal pockets and to reconfirm the effect of periocline on these periodontopathic bacteria. samples from sixty-two periodontal pockets were collected at pocket depths of over 4 mm in twenty-one periodontitis patients aged 43 to 75 years. after sampling, periocline was applied topically to the selected pockets once a week for four weeks and reexamined. the detected rates of the periodontop ...19958583161
in vitro antimicrobial activity of the human neutrophil cytosolic s-100 protein complex, calprotectin, against capnocytophaga sputigena.calprotectin is a complex of two anionic proteins found in abundance in the cytosol of neutrophils, certain macrophages, and oral epithelial keratinocytes. bacteria of the genus capnocytophaga are pathogens of periodontal origin which can cause systemic infection in neutropenic subjects. recently, it has been observed that capnocytophaga may be internalized by neutrophils within the cytosol rather than within a membrane-delimited phagosome. the purpose of this study was to test the in vitro anti ...19938423249
capnocytophaga sputigena bacteremia associated with acute leukemia.during a three-year period, capnocytophaga sputigena bacteremia occurred in three patients with acute leukemia receiving induction therapy on a hematology ward. oral pathology such as periodontitis or severe mucositis was considered to be the most likely source of bacteremia. all three blood culture isolates were identified as that species by deoxyribonucleic acid (dna) homology studies. because of the phenotypical similarity of capnocytophaga species, it is difficult to differentiate them by co ...19938360517
hla-d and t lymphocyte reactivity to specific periodontal pathogens in type 1 diabetic periodontitis.bacterial antigen fragments complexed with class ii major histocompatibility molecules (hla-d) on antigen presenting cells (apcs) stimulate cd4+ t lymphocyte proliferation, presumably to protect the host. this study examined these responses to antigens of two periodontal pathogens in four groups (n = 15) of age- (young adult) and sex-matched caucasian subjects with or without type 1 diabetes and moderate to severe periodontitis: group dp = diabetics with periodontitis; group dnp = diabetics with ...19938277407
bactericidal activities of synthetic human leukocyte cathepsin g-derived antibiotic peptides and congeners against actinobacillus actinomycetemcomitans and capnocytophaga sputigena.actinobacillus actinomycetemcomitans and capnocytophaga spp. are gram-negative bacteria implicated in the etiology of periodontal disease (particularly in individuals with neutrophil defects) and life-threatening systemic infections. they are resistant to many antibiotics of microbial origin but are sensitive to the nonoxidative microbicidal action of neutrophils. these organisms are susceptible to the microbicidal effect of cathepsin g but are killed by two distinct mechanisms. the purpose of t ...19938109940
bacteremia by multidrug-resistant capnocytophaga sputigena.a case of bacteremia caused by a multiresistant strain of capnocytophaga sputigena in a patient with hematological malignancy is described. the strain presented with a pattern of marked resistance to beta-lactams, with mics of > 256 mg/liter for ampicillin, ticarcillin, piperacillin, cefazolin, and cefuroxime, 64 mg/liter for cefotaxime, and 32 mg/liter for ceftazidime. in addition, the mic of ciprofloxacin was 16 mg/liter. both of these groups of antimicrobial agents are frequently used in the ...19948027314
properties of alkaline phosphatase in the gingival crevicular fluid.the isoenzymic properties of the alkaline phosphatase (alp) of the gingival crevicular fluid (gcf) were investigated and compared with those in other cells, such as human polymorphonuclear leukocytes (pmns), and human periodontal ligament cells (pdls), and with those of three species of periodontopathic bacteria: porphyromonas gingivalis 381 (p. gingivalis), prevotella intermedia atcc25611 (p. intermedia), and capnocytophaga sputigena atcc33123 (c. sputigena). the biochemical properties of the i ...19957796488
[aortic endocarditis due to capnocytophaga sputigena]. 19957603114
an approach to efficacy screening of mouthrinses: studies on a group of french products (i). staining and antimicrobial properties in vitro.there is a large and increasing number of oral hygiene products available to the general public. as such, it is difficult to demonstrate efficacy for all in long-term home-use studies. the aim of this project was to determine whether screening studies could position the activity and efficacy of a number of antiseptic mouthrinses, available in france, by comparison with an established product. this study reports the experiments in vitro. the products under test were, 4 containing chlorhexidine (e ...19957593703
microflora in adult periodontitis.the subgingival microflora of adult periodontitis was studied in 8 adults (36-47 years) and compared with that of 10 periodontally healthy individuals (36-43 years). a total of 64 periodontal lesions were examined, and classified according to the attachment level in three categories: attachment loss > 6 mm, attachment loss 4-6 mm and attachment loss < 4 mm. also for comparative purposes 20 gingival sulci were evaluated. samples were taken using three standardized paper points and were incubated ...19957494174
effective method for discriminating between oral bacterial and human alkaline phosphatase activity.alkaline phosphatase (alpase) activity was quantitatively compared in various kinds of oral bacteria. high alpase activity was detected in 3 species of periodontal bacteria, porphyromonas gingivalis, prevotella intermedia and capnocytophaga sputigena. the alpase activity detected in these bacteria was almost completely inhibited in the presence of 1% sodium dodecyl sulfate (sds). by contrast, the activity of mammalian alpase isoenzymes was not inhibited at all even in the presence of 1% sds. the ...19947478753
detection of a fibroblast proliferation inhibitory factor from capnocytophaga sputigena.the addition of a sonic extract of capnocytophaga sputigena to the culture fluid to human fibroblasts resulted in an inhibition of cell proliferation. the inhibition was dose-related (200 micrograms/ml caused a 90% inhibition, and 1,000 micrograms/ml caused a complete cessation of growth). the growth inhibition was not due to alterations in culture medium, ph or ionic strength, or to the effects of the c. sputigena lipopolysaccharide.19807358430
biological and chemical characterization of endotoxin from capnocytophaga sputigena.an endotoxin was isolated from capnocytophaga sputigena strain 4 by a modification of the hot phenol-water method. the extraction procedure yielded a lipopolysaccharide which accounted for approximately 1.5% of the dry weight of the cells. the material was composed of 18.6% lipid (as c(15) fatty acid), 46.5% neutral sugar including 9.6% hexose, 18.3% 6-deoxy sugar, 1.0% 2-keto-3-deoxy sugar, and 4.8% heptose. hexosamine, protein, and phosphorus were found in quantities amounting to 9.0, 2.9, and ...19807358428
[septicemia caused by capnocytophaga sputigena]. 19817290958
cellular fatty acids of capnocytophaga species.the cellular fatty acid composition of 18 clinical isolates and 4 reference strains of capnocytophaga species (capnocytophaga ochracea, capnocytophaga gingivalis, and capnocytophaga sputigena) was determined by gas-liquid chromatography. the fatty acid profiles of the 22 cultures were essentially identical and were characterized by major amounts (60% or greater) of a saturated, iso-branched-chain, 15-carbon acid (13-methyl-tetradecanoate) and the presence of two relatively uncommon saturated, is ...19827153332
[capnocytophaga sputigena in sinusitis].the genus capnocytophaga was reported in the literature for the first time in 1979. capnocytophaga are fusiform gram-negative, slowly growing bacteria that require a high pco2. they belong to the normal buccal flora and they are reported as pathogens in infections of the periodontal region with progressive periodontitis and in septicaemia in patients with immunodeficiencies and buccal ulcerations. the present paper is the first to report capnocytophaga sputigena as a pathogen in sinusitis. the p ...19836670527
sodium fluoride susceptibilities of suspected periodontopathic bacteria.utilizing a blood-based medium, we determined the minimum inhibitory concentrations (mic's) to sodium fluoride (naf) of 45 bacterial strains representing 22 oral species. bacterial susceptibility ranged from 128 micrograms/ml to 2048 micrograms/ml. of those organisms tested, actinobacillus actinomycetemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga ochracea, and actinomyces viscosus were susceptible at lower levels of naf.19836574154
ultrastructural studies of the effect of human leukocyte extracts on periodontopathic bacteria.human gingival crevicular exudate (ge) and leukocyte extracts (le) were previously shown to be able to release the bulk of radioactivity from a variety of 14c-labeled oral bacteria. the present study demonstrates that the release of radiolabel by these enzymatic agents from two periodontopathic bacteria (actinobacillus actinomycetemcomitans strain y4 and capnocytophaga sputigena strain 4) is accompanied by both cell wall and cytoplasmatic damage. the possible role of bacterial products released ...19836345381
effect of human leukocyte extracts and gingival exudate on periodontopathic bacteria.the effect of leukocyte hydrolytic enzymes on periodontopathic bacteria was examined in vitro. a frozen and thawed extract of human peripheral blood leukocytes (le) and human gingival crevicular exudate (ge) were shown to be able to cause the release of 50% of the radioactivity from a leukotoxic strain of actinobacillus actinomycetemcomitans (aa y4), labeled by 14c. a nonleukotoxic strain (aa 653) was shown to be more susceptible to both le and ge, up to 68% of the total radioactivity was solubi ...19836345380
biological and chemical comparison of butanol- and phenol-water extracted lipopolysaccharide from capnocytophaga sputigena. 19836227738
clinical and laboratory study of a new diuretic agent ("vectren") in pregnancy: a comparison with a diuretic agent in current use ("enduron"). 20164889612
abnormal postural reflexes. 20164847456
medical education, medical care, and society in sweden. 20164818471
[treatment of herpes zoster with emetine]. 20164767947
bone-conduction threshold sensitivity in relation to mastoid sclerosis. 20154744955
a rapid method for the electrophoresis of chromatin proteins. 19734723239
[study of the chemical composition of low-protein diets for patients with chronic renal insufficiency]. 20144664451
the profession of medicine. 20154644652
degree of human genetic isolation measured by isonymy and marital distances in two communities in an italian alpine valley. 19724636780
[the role of alcohol in therapy]. 20154601159
lipid composition of xanthomas of different types. 20154576167
suppressor cells in the rabbit appendix. 19744547314
[problems of values and objectives in planned change]. 20154531521
[late results after surgery of temporomandibular joint luxation by mucosa excision and preparation of parapharyngeal pockets]. 20154508535
training of practical work instructors. 20154495649
"no more home deliveries". 20154485328
amino acid sequence of a peptide containing the active cysteine residue of histidine ammonia-lyase.1. oxidized (polymerized) histidine ammonia-lyase from pseudomonas testosteroni was activated with dithiothreitol and the reduced disulphide-linked cysteine residues of the native enzyme were carboxymethylated with iodo[(14)c]acetate. 2. the activity of the carboxymethylated enzyme was similar to that of the polymerized form and approx. 15% of that of the fully reduced form. 3. a tryptic digest of the [(14)c]carboxymethylated enzyme contained only one radioactive peptide. 4. the amino acid seque ...19744422650
inhibition of hepatic ribonuclease activity by chronic administration of phenobarbitone. 19704394767
the paraproteinemias. ii. 20154380090
adhesive strips in the closure of wounds in outpatient surgery in children. 20154378984
[alpha fetoprotein in hemorrhagic ascitic fluid in a case of hepatocellular carcinoma]. 20154356944
some pharmacological properties of a synthetic oxytocin analogue [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), an antagonist to the neurohypophysial hormones.1. a synthetic oxytocin analogue, [1-n-carbamoyl-hemicystine-2-o-methyltyrosine]-oxytocin (carbamoyl-methyloxytocin), has been tested as an antagonist to the actions of oxytocin and vasopressin on the uterus, the mammary gland and blood pressure.2. the analogue inhibited the response of the isolated rat uterus to both oxytocin and vasopressin without itself stimulating the uterus to contract. the responses to equipotent doses of oxytocin and vasopressin were inhibited equally. there was little o ...19704321760
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