Publications

effects of the dietary protein and carbohydrate ratio on gut microbiomes in dogs of different body conditions.	obesity has become a health epidemic in both humans and pets. a dysbiotic gut microbiota has been associated with obesity and other metabolic disorders. high-protein, low-carbohydrate (hplc) diets have been recommended for body weight loss, but little is known about their effects on the canine gut microbiome. sixty-three obese and lean labrador retrievers and beagles (mean age, 5.72 years) were fed a common baseline diet for 4 weeks in phase 1, followed by 4 weeks of a treatment diet, specifical ...	2017	28119466
oral administration of a select mixture of bacillus probiotics affects the gut microbiota and goblet cell function following escherichia coli challenge in newly weaned pigs of genotype muc4 that are supposed to be enterotoxigenic e. coli f4ab/ac receptor negative.	structural disruption of the gut microbiota and impaired goblet cell function are collateral etiologic factors in enteric diseases. low, moderate, or high doses of a bacillus licheniformis-b. subtilis mixture (bls mix) were orally administered to piglets of genotype muc4 that are supposed to be f4-expressing enterotoxigenic escherichia coli strain (f4(+) etec) f4ab/ac receptor negative (i.e., muc4-resistant piglets) for 1 week before f4(+) etec challenge. the luminal contents were collected from ...	2017	27881419
identification of vaginal fluid, saliva, and feces using microbial signatures in a han chinese population.	in recent years, forensic scientists have focused on the discrimination of body fluids using microbial signatures. in this study, we performed pcr-based detection of microbial signatures of vaginal fluid, saliva, and feces in a han chinese population. we investigated the 16s rrna genes of lactobacillus crispatus, lactobacillus gasseri, lactobacillus jensenii, lactobacillus iners, and atopobium vaginae in vaginal fluid, the 16s rrna and the glucosyltransferase enzyme genes of streptococcus saliva ...	2016	27570236
bacteroidales secreted antimicrobial proteins target surface molecules necessary for gut colonization and mediate competition in vivo.	we recently showed that human gut bacteroidales species secrete antimicrobial proteins (bsaps), and we characterized in vitro the first such bsap produced by bacteroides fragilis in this study, we identified a second potent bsap produced by the ubiquitous and abundant human gut species bacteroides uniformis the two bsaps contain a membrane attack complex/perforin (macpf) domain but share very little sequence similarity. we identified the target molecules of bsap-sensitive cells and showed that e ...	2016	27555309
microbial community of healthy thai vegetarians and non-vegetarians, their core gut microbiota, and pathogen risk.	pyrosequencing analysis of intestinal microflora from healthy thai vegetarians and non-vegetarians exhibited 893 otus covering 189 species. the strong species indicators of vegetarians and non-vegetarians were prevotella copri and bacteroides vulgatus as well as bacteria close to escherichia hermanii with % relative abundance of 16.9 and 4.5-4.7, respectively. core gut microbiota of the vegetarian and non-vegetarian groups consisted of 11 and 20 different bacterial species, respectively, belongi ...	2016	27381339
distinctly altered gut microbiota in the progression of liver disease.	recent studies underscore important roles of intestinal microbiota and the bacterial lipopolysaccharides (lps) production in the pathogenesis of liver disease. however, how gut microbiota alters in response to the development of steatosis and subsequent progression to nonalcoholic steatohepatitis (nash) and hepatocellular carcinoma (hcc) remains unclear. we aimed to study the gut microbial changes over liver disease progression using a streptozotocin-high fat diet (stz-hfd) induced nash-hcc c57b ...	2016	27036035
reduced abundance of butyrate-producing bacteria species in the fecal microbial community in crohn's disease.	the global alteration of the gut microbial community (dysbiosis) plays an important role in the pathogenesis of inflammatory bowel diseases (ibds). however, bacterial species that characterize dysbiosis in ibd remain unclear. in this study, we assessed the alteration of the fecal microbiota profile in patients with crohn's disease (cd) using 16s rrna sequencing.	2016	26789999
safety assessment of bacteroides uniformis cect 7771 isolated from stools of healthy breast-fed infants.	bacteroides uniformis cect 7771 is a potential probiotic strain, originally isolated from the stools of healthy breast-feed infants. the strain showed pre-clinical efficacy in a mouse obesity model. the objective of this study was to evaluate its potential toxicity and translocation ability after acute oral administration to mice.	2016	26784747
brain abscess due to aggregatibacter aphrophilus and bacteroides uniformis.	the aim of this report was to describe the occurrence of a bacterial brain abscess in a healthy individual, without any predisposing condition.	2015	26702912
tnfr2 deficiency acts in concert with gut microbiota to precipitate spontaneous sex-biased central nervous system demyelinating autoimmune disease.	tnf-α antagonists provide benefit to patients with inflammatory autoimmune disorders such as crohn's disease, rheumatoid arthritis, and ankylosing spondylitis. however, tnf antagonism unexplainably exacerbates cns autoimmunity, including multiple sclerosis and neuromyelitis optica. the underlying mechanisms remain enigmatic. we demonstrate that tnfr2 deficiency results in female-biased spontaneous autoimmune cns demyelination in myelin oligodendrocyte glycoprotein-specific 2d2 tcr transgenic mic ...	2015	26475926
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents - special references to bacteria isolated between april 2011 and march 2012].	bacteria isolated from surgical infections during the period from april 2011 to march 2012 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 785 strains including 31 strains of candida spp. were isolated from 204 (78.8%) of 259 patients with surgical infections. five hundred and twenty three strains were isolated from primary infections, and 231 strains were isolated from surgical site infection. from primary infections, anaerobic gram-ne ...	2014	25796741
structure-guided functional characterization of duf1460 reveals a highly specific nlpc/p60 amidase family.	glcnac-1,6-anhydro-murnac-tetrapeptide is a major peptidoglycan degradation intermediate and a cytotoxin. it is generated by lytic transglycosylases and further degraded and recycled by various enzymes. we have identified and characterized a highly specific n-acetylmuramoyl-l-alanine amidase (amia) from bacteroides uniformis, a member of the duf1460 protein family, that hydrolyzes glcnac-1,6-anhydro-murnac-peptide into disaccharide and stem peptide. the high-resolution apo structure at 1.15 å re ...	2014	25465128
evidence for extraintestinal growth of bacteroidales originating from poultry litter.	water quality monitoring techniques that target microorganisms in the order bacteroidales are potential alternatives to conventional methods for detection of fecal indicator bacteria. bacteroidales and members of the genus bacteroides have been the focus of microbial source tracking (mst) investigations for discriminating sources of fecal pollution (e.g., human or cattle feces) in environmental waters. for accurate source apportionment to occur, one needs to understand both the abundance of bact ...	2015	25326306
crystal structures of three representatives of a new pfam family pf14869 (duf4488) suggest they function in sugar binding/uptake.	crystal structures of three members (bacova_00364 from bacteroides ovatus, bacuni_03039 from bacteroides uniformis and bacegg_00036 from bacteroides eggerthii) of the pfam domain of unknown function (duf4488) were determined to 1.95, 1.66, and 1.81 å resolutions, respectively. the protein structures adopt an eight-stranded, calycin-like, β-barrel fold and bind an endogenous unknown ligand at one end of the β-barrel. the amino acids interacting with the ligand are not conserved in any other prote ...	2014	25044324
dynamic microbe and molecule networks in a mouse model of colitis-associated colorectal cancer.	bacterial colonisation of the gut is involved in the development of colitis-associated colorectal cancer. however, it remains unclear how the gut microbiota dynamically shifts correlating with colorectal carcinogenesis. here, we reveal the longitudinal shifts in the microbial community that occur with colitis-associated colorectal cancer. high-throughput sequencing results for the bacterial 16s rrna gene (v3 region) were compared for azoxymethane/dextran sodium sulphate-treated mice and control ...	2014	24828543
senior thai fecal microbiota comparison between vegetarians and non-vegetarians using pcr-dgge and real-time pcr.	the fecal microbiotas were investigated in 13 healthy thai subjects using polymerase chain reaction denaturing gradient gel electrophoresis (pcr-dgge). among the 186 dna bands detected on the polyacrylamide gel, 37 bands were identified as representing 11 species: bacteroides thetaiotaomicron, bacteroides ovatus, bacteroides uniformis, bacteroides vulgatus, clostridium colicanis, eubacterium eligenes, e. rectale, faecalibacterium prausnitzii, megamonas funiformis, prevotella copri, and roseburia ...	2014	24743571
isolation and characterization of an agaro-oligosaccharide (ao)-hydrolyzing bacterium from the gut microflora of chinese individuals.	agarose (ap) from red algae has a long history as food ingredients in east asia. agaro-oligosaccharides (ao) derived from ap have shown potential prebiotic effects. however, the human gut microbes responsible for the degradation of ao and ap have not yet been fully investigated. here, we reported that ao and ap can be degraded and utilized at various rates by fecal microbiota obtained from different individuals. bacteroides uniformis l8 isolated from human feces showed a pronounced ability to de ...	2014	24622338
disrupted intestinal microbiota and intestinal inflammation in children with cystic fibrosis and its restoration with lactobacillus gg: a randomised clinical trial.	intestinal inflammation is a hallmark of cystic fibrosis (cf). administration of probiotics can reduce intestinal inflammation and the incidence of pulmonary exacerbations. we investigated the composition of intestinal microbiota in children with cf and analyzed its relationship with intestinal inflammation. we also investigated the microflora structure before and after lactobacillus gg (lgg) administration in children with cf with and without antibiotic treatment.	2014	24586292
rna-stable-isotope probing shows utilization of carbon from inulin by specific bacterial populations in the rat large bowel.	knowledge of the trophisms that underpin bowel microbiota composition is required in order to understand its complex phylogeny and function. stable-isotope ((13)c)-labeled inulin was added to the diet of rats on a single occasion in order to detect utilization of inulin-derived substrates by particular members of the cecal microbiota. cecal digesta from fibruline-inulin-fed rats was collected prior to (0 h) and at 6, 12, 18 and 24 h following provision of the [(13)c]inulin diet. rna was extracte ...	2014	24487527
isolation of bacteroides from fish and human fecal samples for identification of unique molecular markers.	bacteroides molecular markers have been used to identify human fecal contamination in natural waters, but recent work in our laboratory confirmed cross-amplification of several human-specific bacteroides spp. assays with fecal dna from fish. for identification of unique molecular markers, bacteroides from human (n = 4) and fish (n = 7) fecal samples were cultured and their identities were further confirmed using rapid id 32a api strips. the 16s rdna from multiple isolates from each sample was pc ...	2013	24313449
future for probiotic science in functional food and dietary supplement development.	the purpose of this study is to provide an update of probiotic science evolving from classical approaches to the development of next-generation probiotics, parallel to advances in the understanding of the complexity of the gut microbiome and its role in human health.	2013	24071779
cloning, expression, and characterization of the β-glucosidase hydrolyzing secoisolariciresinol diglucoside to secoisolariciresinol from bacteroides uniformis zl1.	previously, from the human intestinal flora we isolated the bacterial strain bacteroides uniformis zl1, which could convert secoisolariciresinol diglucoside (sdg) to its aglycone secoisolariciresinol (seco) in vivo. in this study, 24 putative β-glucosidase genes were screened from the genome of b. uniformis atcc 8492, which were used as templates to design pcr primers for the target genes in b. uniformis zl1. fifteen genes (bgl1-bgl15) were amplified from strain zl1, and among them we identified ...	2014	23942877
multi-centre evaluation of mass spectrometric identification of anaerobic bacteria using the vitek® ms system.	accurate and timely identification of anaerobic bacteria is critical to successful treatment. classic phenotypic methods for identification require long turnaround times and can exhibit poor species level identification. matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) is an identification method that can provide rapid identification of anaerobes. we present a multi-centre study assessing the clinical performance of the vitek(®) ms in the identification ...	2014	23927597
ethnic diversity of gut microbiota: species characterization of bacteroides fragilis group and genus bifidobacterium in healthy belgian adults, and comparison with data from japanese subjects.	the composition of the human gut microbiota is related to host health, and it is thought that dietary habits may play a role in shaping this composition. here, we examined the population size and prevalence of six predominant bacterial genera and the species compositions of genus bifidobacterium (g-bifid) and bacteroides fragilis group (g-bfra) in 42 healthy belgian adults by quantitative pcr (qpcr) over a period of one month. the population sizes and prevalence of these bacteria were basically ...	2013	23522670
temporal change in the gut community of rats fed high amylose cornstarch is driven by endogenous urea rather than strictly on carbohydrate availability.	to examine change in the gut community of rats fed high amylose maize starch (hams).	2013	23383759
analysis of a novel 8.9kb cryptic plasmid from bacteroides uniformis, its long-term stability and spread within human microbiota.	the analysis of plasmid content in dominant bacteroidales order intestinal strains isolated from the same child at a 5 year interval identified a 8.9 kb plasmid in bacteroides uniformis bun24 strain isolated at age 6 and indistinguishably sized plasmids in the isolates of b. uniformis, b. vulgatus, b. intesinalis, and parabacteroides distasonis at age 11. we sequenced a b. uniformis bun24 plasmid, designated pbun24, and using molecular surveys of diverse species we established that this 8944bp m ...	2013	23201047
identification of feces by detection of bacteroides genes.	in forensic science, the identification of feces is very important in a variety of crime investigations. however, no sensitive and simple fecal identification method using molecular biological techniques has been reported. here, we focused on the fecal bacteria, bacteroides uniformis, bacteroides vulgatus and bacteroides thetaiotaomicron, and developed a novel fecal identification method by detection of the gene sequences specific to these bacteria in various body (feces, blood, saliva, semen, u ...	2013	23068949
production of secoisolariciresinol from defatted flaxseed by bacterial biotransformation.	secoisolariciresinol (seco) is increasingly recognized for potential clinical application because of its preventive effects against breast and colon cancers, atherosclerosis and diabetes, and its production through biotransformation has been attempted. however, previously reported bacteria all required stringent anaerobic culture conditions, precluding large-scale production. here, we report the isolation and characterization of bacteria that produce seco under less stringent anaerobic culture c ...	2012	22924993
bacteroides uniformis cect 7771 ameliorates metabolic and immunological dysfunction in mice with high-fat-diet induced obesity.	associations have been made between obesity and reduced intestinal numbers of members of the phylum bacteroidetes, but there is no direct evidence of the role these bacteria play in obesity. herein, the effects of bacteroides uniformis cect 7771 on obesity-related metabolic and immune alterations have been evaluated.	2012	22844426
influence of red wine polyphenols and ethanol on the gut microbiota ecology and biochemical biomarkers.	few studies have investigated the effect of dietary polyphenols on the complex human gut microbiota, and they focused mainly on single polyphenol molecules and select bacterial populations.	2012	22552027
mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis.	to characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method.	2012	22529692
bioinformatic evidence and characterization of novel putative large conjugative transposons residing in genomes of genera bacteroides and prevotella.	bioinformatic evidence of the presence of a large conjugative transposon in ruminal bacterium prevotella bryantii b(1)4(t) is presented. the described transposon appears to be related to another large conjugative transposon ctnbst, described in bacteroides uniformis wh207 and to the conjugative transposon ctn3-bf, which was observed in the genome of bacteroides fragilis strain ych46. all three transposons share tra gene regions with high amino acid identity and clearly conserved gene order. addi ...	2012	22528303
structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers.	despite a long-suspected role in the development of human colorectal cancer (crc), the composition of gut microbiota in crc patients has not been adequately described. in this study, fecal bacterial diversity in crc patients (n=46) and healthy volunteers (n=56) were profiled by 454 pyrosequencing of the v3 region of the 16s ribosomal rna gene. both principal component analysis and unifrac analysis showed structural segregation between the two populations. forty-eight operational taxonomic units ...	2011	21850056
colonization of infant's gut by bacteroides is influenced by environmental and genetic factors linked to celiac disease risk.	celiac disease (cd) is an immune-mediated naturopathy involving genetic and environmental factors, whose interaction might influence disease risk. the aim of this study was to determine the effects of milk-feeding practices and hla-dq genotype on intestinal colonization of bactericides species in infants at risk of cd development. this study included 75 full-term newborns with at least one first-degree relative suffering cd. infants were classified according to their milk-feeding practices (brea ...	2011	21642397
bacteroides uniformis is a putative bacterial species associated with the degradation of the isoflavone genistein in human feces.	inter-individual variation in isoflavone absorption depends on gut microbial degradation and affects the efficacy of these compounds. we hypothesized that inter-individual variation in fecal isoflavone disappearance coincided with variation in bacterial species. in vitro anaerobic fecal disappearance of isoflavones was measured from 33 participants by hplc. fecal microbial 16s rrna variable region pcr products were obtained from 4 participants with the greatest and least genistein or glycitein d ...	2011	21525249
application of quantitative real-time pcr for rapid identification of bacteroides fragilis group and related organisms in human wound samples.	our goal was to establish a quantitative real-time pcr (qrt-pcr) method to detect bacteroides fragilis group and related organisms from clinical specimens. compared to conventional anaerobic culture, qrt-pcr can provide accurate and more rapid detection and identification of b.-áfragilis group and similar species. b.-áfragilis group and related organisms are the most frequently isolated anaerobic pathogens from clinical samples. however, culture and phenotypic identification is quite time-consum ...	2011	21439390
longitudinal analysis of the prevalence, maintenance, and iga response to species of the order bacteroidales in the human gut.	bacteroidales species are the most abundant gram-negative bacteria of the human intestinal microbiota. these bacteria evolved to synthesize numerous capsular polysaccharides (ps) that are subject to phase variation. in bacteroides fragilis, ps synthesis is regulated so that only one of the eight ps biosynthesis loci is transcribed at a time in each bacterium. to determine if the bacteria evolved this unusual property to evade a host iga response, we directly studied the human fecal ecosystem. we ...	2011	21402766
intestinal bacteroides species associated with coeliac disease.	to characterise the predominant species of bacterial populations associated with duodenal biopsies of paediatric patients with active and treated coeliac disease.	2010	20972239
bacteroides chinchillae sp. nov. and bacteroides rodentium sp. nov., isolated from chinchilla (chinchilla lanigera) faeces.	gram-negative anaerobic rods were isolated from chinchilla (chinchilla lanigera) faeces and three strains, st170(t), st180 and st28(t), were investigated taxonomically. on the basis of phylogenetic analyses and specific phenotypic characteristics, the three strains belonged to the genus bacteroides. phylogenetic analysis of their 16s rrna gene sequences revealed that strains st170(t) and st180 formed a single cluster and a distinct line of descent. strain st170(t) exhibited 99.7 % 16s rrna gene ...	2011	20495039
lessons learned from the anaerobe survey: historical perspective and review of the most recent data (2005-2007).	the rationale and lessons learned through the evolution of the national survey for the susceptibility of bacteroides fragilis group from its initiation in 1981 through 2007 are reviewed here. the survey was conceived in 1980 to track emerging antimicrobial resistance in bacteroides species.	2010	20067390
bacteroides clarus sp. nov., bacteroides fluxus sp. nov. and bacteroides oleiciplenus sp. nov., isolated from human faeces.	three gram-stain-negative, obligately anaerobic, non-spore-forming, rod-shaped bacteria (strains yit 12056t, yit 12057t and yit 12058t) were isolated from human faeces. these strains were characterized by phylogenetic analyses based on 16s rrna gene sequence and phenotypic tests. 16s rrna gene sequence analyses revealed that strains yit 12056t, yit 12057t and yit 12058t were most closely related to the type strains of bacteroides gallinarum, bacteroides uniformis and bacteroides intestinalis wit ...	2010	19767355
species identification of clinical isolates of bacteroides by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry.	bacteroides fragilis and related species are important human pathogens involved in mixed infections of different origins. the b. fragilis group isolates are phenotypically very similar, grow more slowly than aerobic bacteria and, accordingly, are frequently misidentifed with classical or automated phenotypical identification methods. recent taxonomic changes and new species accepted as members of the bacteroides genus are not included in the different databases of commercially available identifi ...	2009	19438622
moxifloxacin resistance is prevalent among bacteroides and prevotella species in greece.	moxifloxacin is recommended in the empirical treatment of infections involving gram-negative anaerobes. however, current european data regarding its activity against anaerobic pathogens are limited. in order to evaluate its potency, we comparatively studied the activity of moxifloxacin against recently isolated gram-negative anaerobes.	2008	18385145
succession in the intestinal microbiota of preadolescent turkeys.	in the present study, automated ribosomal intergenic spacer analysis (arisa), library sequence analysis, real-time pcr detection of bacteroides uniformis and campylobacter coli and dot-blot hybridizations of clostridiaceae were used to identify trends in microbial colonization of the ceca of male turkeys. two separate trials were performed with six and five birds, respectively. arisa community profiles identified a period of community transition at week 12 of age in both trials. a significant in ...	2007	17284250
bacteroides barnesiae sp. nov., bacteroides salanitronis sp. nov. and bacteroides gallinarum sp. nov., isolated from chicken caecum.	eight bacterial strains isolated from the caecum of chicken, bl2(t), bl66, eg3, eg6, m27, bl78(t), c35(t) and c43, were characterized by determining their phenotypic characteristics, cellular fatty acid profiles, menaquinone profiles and phylogenetic positions based on 16s rrna gene sequence analysis. 16s rrna gene sequence analysis showed that these isolates belonged to the genus bacteroides. one group of five strains (bl2(t), bl66, eg3, eg6 and m27) was related most closely to bacteroides copr ...	2006	17158987
bacteroides intestinalis sp. nov., isolated from human faeces.	during studies of the microbiota of human faeces, five strains of gram-negative anaerobic rods were isolated following growth in a polyamine-deficient medium. these strains belonged to the genus bacteroides on the basis of 16s rrna gene sequence data. 16s rrna gene sequence similarity between one of the strains, 341t, and recognized species within the genus bacteroides was <95 %. the dna g+c content (44 mol%) and major fatty acid composition (anteiso-c(15 : 0), 32.0 %) supported the affiliation ...	2006	16403880
effects of difructose anhydride iii (dfa iii) administration on rat intestinal microbiota.	the effects of difructose anhydride iii (di-d-fructofuranose-1,2':2,3'-dianhydride; dfa iii) administration (3% dfa iii for 4 weeks) on rat intestinal microbiota were examined using denaturing gradient gel electrophoresis (dgge). according to dgge profiles, the number of bacteria related to bacteroides acidofaciens and uncultured bacteria within the clostridium lituseburense group decreased, while that of bacteria related to bacteroides vulgatus, bacteroides uniformis and ruminococcus productus ...	2005	16233782
spontaneous spondylodiscitis caused by bacteroides uniformis.		2004	15308443
a new bacteroides conjugative transposon that carries an ermb gene.	the erythromycin resistance gene ermb has been found in a variety of gram-positive bacteria. this gene has also been found in bacteroides species but only in six recently isolated strains; thus, the gene seems to have entered this genus only recently. one of the six bacteroides ermb-containing isolates, wh207, could transfer ermb to bacteroides thetaiotaomicron strain bt4001 by conjugation. wh207 was identified as a bacteroides uniformis strain based on the sequence of its 16s rrna gene. results ...	2003	14602600
a comparison of five methods for extraction of bacterial dna from human faecal samples.	the purity of dna extracted from faecal samples is a key issue in the sensitivity and usefulness of biological analyses such as pcr for infectious pathogens and non-pathogens. we have compared the relative efficacy of extraction of bacterial dna (both gram negative and positive origin) from faeces using four commercial kits (fastdna kit, bio 101; nucleospin c+t kit, macherey-nagal; quantum prep aquapure genomic dna isolation kit, bio-rad; qiaamp dna stool mini kit, qiagen) and a non-commercial g ...	2002	11997164
in vitro activity of gemifloxacin compared to seven other oral antimicrobial agents against aerobic and anaerobic pathogens isolated from antral sinus puncture specimens from patients with sinusitis.	using a microbroth method for aerobes and agar dilution for anaerobes, we studied the comparative in vitro activity of gemifloxacin, three fluoroquinolones, two macrolides and two beta-lactams, against 207 aerobic and 162 anaerobic antral sinus puncture isolates. gemifloxacin was active at < or = 0.25 ug/ml against 198/207 [96%] aerobes and 127/162 [78%] anaerobes or 325/369 [88%] of all isolates and was the most active quinolone on a weight basis against gram-positive organisms. all haemophilus ...	2002	11858906
gyra mutations associated with quinolone resistance in bacteroides fragilis group strains.	mutations in the gyra gene contribute considerably to quinolone resistance in escherichia coli. mechanisms for quinolone resistance in anaerobic bacteria are less well studied. the bacteroides fragilis group are the anaerobic organisms most frequently isolated from patients with bacteremia and intraabdominal infections. forty-four clinafloxacin-resistant and-susceptible fecal and clinical isolates of the b. fragilis group (eight bacteroides fragilis, three bacteroides ovatus, five bacteroides th ...	2001	11408211
multicenter survey of the changing in vitro antimicrobial susceptibilities of clinical isolates of bacteroides fragilis group, prevotella, fusobacterium, porphyromonas, and peptostreptococcus species.	in vitro surveys of antimicrobial resistance among clinically important anaerobes are an important source of information that can be used for clinical decisions in the choice of empiric antimicrobial therapy. this study surveyed the susceptibilities of 556 clinical anaerobic isolates from four large medical centers using a broth microdilution method. piperacillin-tazobactam was the only antimicrobial agent to which all the isolates were susceptible. similarly, imipenem, meropenem, and metronidaz ...	2001	11257040
comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria.	the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ...	2001	11120995
tla-1: a new plasmid-mediated extended-spectrum beta-lactamase from escherichia coli.	escherichia coli r170, isolated from the urine of an infected patient, was resistant to expanded-spectrum cephalosporins, aztreonam, ciprofloxacin, and ofloxacin but was susceptible to amikacin, cefotetan, and imipenem. this particular strain contained three different plasmids that encoded two beta-lactamases with pis of 7.0 and 9.0. resistance to cefotaxime, ceftazidime, aztreonam, trimethoprim, and sulfamethoxazole was transferred by conjugation from e. coli r170 to e. coli j53-2. the transfer ...	2000	10722503
genetic-biochemical analysis and distribution of the ambler class a beta-lactamase cme-2, responsible for extended-spectrum cephalosporin resistance in chryseobacterium (flavobacterium) meningosepticum.	in vitro synergy between extended-spectrum cephalosporins and either clavulanic acid or cefoxitin was found for chryseobacterium meningosepticum isolates during a double-disk assay on an agar plate. an extended-spectrum beta-lactamase (esbl) gene from a c. meningosepticum clinical isolate was cloned and expressed in escherichia coli dh10b. its protein conferred resistance to most beta-lactams including extended-spectrum cephalosporins but not to cephamycins or to imipenem. its activity was stron ...	2000	10602714
in vitro activity of gemifloxacin (sb 265805) against anaerobes.	gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ...	1999	10471570
stimulation of transforming growth factor beta1 by enteric bacteria in the pathogenesis of rat intestinal fibrosis.	bacteria and their products stimulate inflammatory responses. certain mediators, such as transforming growth factor beta1 (tgf-beta1), induce collagen synthesis. excess collagen deposition results in bowel strictures. the aim of this study was to investigate the role of bacteria and tgf-beta1 in the pathogenesis of intestinal fibrosis.	1998	9496942
characterization of saccharolytic bacteroides and prevotella isolates from infected dog and cat bite wounds in humans.	saccharolytic, nonpigmented, anaerobic gram-negative rods isolated from infected dog and cat bite wounds in humans have been poorly characterized, and most are not included in the databases of kits used for anaerobic identification; thus, they are problematic for clinical laboratories to identify. fifty strains isolated from such wounds were characterized with commercial kits for preformed-enzyme detection, carbohydrate fermentation, and other biochemical tests. pcr fingerprinting was performed ...	1997	9003606
a case of subacute necrotizing fasciitis.	we report a 48-year-old woman who developed necrotizing groin fasciitis with insidious onset. before she visited us, she had been unsuccessfully treated with several kinds of antibiotics by other doctors for one month, because of a small ulcer covered by blackish necrotic tissue. she was referred to us because of high fever, an ulcer on the left labium majus, and a cellulitis-like lesion with severe pain on the lower abdomen. methicillin-resistant staphylococcus aureus (mrsa), streptococcus inte ...	1995	8648007
use of a modified bacteroides-prevotella shuttle vector to transfer a reconstructed beta-1,4-d-endoglucanase gene into bacteroides uniformis and prevotella ruminicola b(1)4.	a carboxymethyl cellulase (cmcase) gene from prevotella ruminicola b(1)4 was reconstructed by adding a cellulose binding domain from a thermomonospora fusca cellulase and was conjugally transferred from escherichia coli to bacteroides uniformis 0061 by using a chloramphenicol and tetracycline resistance shuttle vector (ptc-cow). ptc-cow was specifically constructed to facilitate conjugal transfer of vectors from b. uniformis donors to p. ruminicola recipients. b. uniformis transconjugants contai ...	1996	8572695
transfer of hybrid plasmids based on the replicon prri7 from escherichia coli to bacteroides and prevotella strains.	new shuttle vectors based on a prevotella ruminicola 9.5 kb cryptic plasmid (prri7) inserted within the escherichia coli vector pkc71, carrying the ccr/emr bacteroides marker, were constructed. these constructs (pkbr23-1 and pkbr23-2) were transferred into bacteriodes distasonis, bacteroides thetaiotaomicron, bacteroides uniformis and into p. ruminicola ncfb 2202 either by conjugal mobilization or by electroporation. another prri7 derivative based on pkc72, pkbr23-3, was smaller (13.1 kb) and no ...	1993	8486560
molecular and genetic analysis of the bacteroides uniformis cephalosporinase gene, cbla, encoding the species-specific beta-lactamase.	the gene, cbla, encoding the species-specific, clavulanate-susceptible, endogenous cephalosporinase was cloned from bacteroides uniformis wal-7088. the nucleotide sequence was determined, and the cbla structural gene was found to be 891 nucleotides, with a 48% g+c composition, which is similar to that of the b. uniformis genome. the cbla open reading frame encoded an ambler class a beta-lactamase polypeptide precursor of 296 amino acid residues with a predicted molecular weight of 33,450. a beta ...	1994	7985999
cloning and expression of the bacteroides fragilis ych46 neuraminidase gene in escherichia coli and bacteroides uniformis.	a neuraminidase-encoding gene nanh of bacteroides fragilis strain ych46 was cloned into the cosmid vector phc79. the nanh gene was subcloned from the cosmid and was located within a 2.2-kb xhoi-kpni fragment. southern hybridization experiments demonstrated that the gene was present as a single copy on the bacterial chromosome. neuraminidase activity expressed in the initial escherichia coli clone was approximately 3600-fold lower than that expressed in b. fragilis ych46. however, when nanh was t ...	1994	7926664
unexpected sequence similarity between nucleosidases and phosphoribosyltransferases of different specificity.	amino acid sequences of enzymes that catalyze hydrolysis or phosphorolysis of the n-glycosidic bond in nucleosides and nucleotides (nucleosidases and phosphoribosyltransferases) were explored using computer methods for database similarity search and multiple alignment. two new families, each including bacterial and eukaryotic enzymes, were identified. family i consists of escherichia coli amp hydrolase (amn), uridine phosphorylase (udp), purine phosphorylase (deod), uncharacterized proteins from ...	1994	7920254
effect of h2-receptor antagonists on bile acid metabolism.	several reports have been presented concerning pronounced overgrowth of bacteria in gastric juices of patients treated with h2-receptor antagonists. however, there has been no report concerning influence of h2-receptor antagonists on jejunal flora. thus, to investigate the influence and its effect on bile acid metabolism, this study was performed: 1) to examine whether patients with gastric ulcers who have been treated with h2-receptor antagonists have positive bile acid breath tests due to bact ...	1995	7735920
expression of a cloned cellulase/xylanase gene from prevotella ruminicola in bacteroides vulgatus, bacteroides uniformis and prevotella ruminicola.	a new shuttle vector, prh3 (8.7 kb), was constructed for use in prevotella/bacteroides host strains. this vector combines the prri2 replicon from p. ruminicola, pbluescript sequences and a tetq marker gene for selection in prevotella/bacteroides hosts. following insertion of a fragment carrying an endoglucanase/xylanase gene from p. ruminicola 23 into the multiple cloning site, the resulting construct, prh3x, was introduced into b. vulgatus 1447, b. uniformis 1100 and p. ruminicola 2202. this re ...	1995	7592134
purification and characterization of a new beta-lactamase from bacteroides uniformis.	a beta-lactam-resistant bacteroides uniformis strain was isolated from a clinical specimen. the strain produced large amounts of beta-lactamase and was resistant to penicillins and cephalosporins. the specific activity of the unpurified beta-lactamase was 4.8 u/mg of protein with nitrocefin as the substrate. the enzyme was purified 188-fold by q-sepharose, sephacryl s-300, and mono q column passages. kinetic parameters of the enzyme were determined by a micromethod performed in microtiter plates ...	1995	7492085
characterization of the cecal bacteria of normal pigs.	one hundred ninety-two isolates from cecal contents of three normal weaned pigs were obtained by means of anaerobic roll tube methods and were characterized. seventy-eight percent of the isolates were gram-negative. the most numerous species isolated from each of the pigs was bacteroides ruminicola. this species accounted for 35% of the isolates that were characterized, and selenomonas ruminantium accounted for 21% of the isolates. other gram-negative bacteria isolated from all three pigs were b ...	1981	7235711
novel antibiotic resistance transfer in bacteroides.	resistance to tetracycline and lincosamide antibiotics was transferred en bloc from a strain of bacteroides fragilis (v503) to a plasmidless strain of bacteroides uniformis (v528) during in vitro filter matings. resistance transfer was detected at frequencies of 10(-5) to 10(-6) drug-resistant progeny per input donor cell and was dependent on cell-to-cell contact of donors and recipients. transfer was insensitive to dnase and was not mediated by chloroform- or filter-sterilized donor broth cultu ...	1982	7081969
specific strains of bacteroides species in human fecal flora as measured by deoxyribonucleic acid homology.	membrane competition homology experiments were used to compare bacteroides uniformis and bacteroides vulgatus isolates obtained from fecal samples from different individuals and isolates obtained from fecal samples of single individuals. isolates of b. uniformis, when isolated from different individuals, had interstrain deoxyribonucleic acid homology values that ranged from 63 to 95%, with most of the values being in the 70 to 85% range. when isolates obtained from a single individual were compa ...	1980	6990866
characterization of three different beta-lactamases from the bacteroides fragilis group.	beta-lactamases from five strains of bacteroides fragilis and two strains of bacteroides uniformis, all resistant to beta-lactam antibiotics, were compared by means of isoelectric focusing and enzyme kinetic measurements. beta-lactamases from the five b. fragilis strains were identical, whereas those from the two b. uniformis strains were distinguished from each other and also from the b. fragilis enzymes. the two b. uniformis strains were relatively resistant to cefoxitin (minimal inhibitory co ...	1980	6969573
use of randomly cloned dna fragments for identification of bacteroides thetaiotaomicron.	randomly cloned fragments of dna from bacteroides thetaiotaomicron were used as hybridization probes for differentiation of b. thetaiotaomicron from closely related bacteroides species. hindiii digestion fragments of dna from b. thetaiotaomicron (type strain) were inserted into plasmid pbr322 and labeled with [alpha-32p]dctp by nick translation. these labeled plasmids were screened for hybridization to hindiii digests of chromosomal dna from type strains of the following human colonic bacteroide ...	1983	6833179
evidence for a membrane-bound form of a bacteriocin of bacteroides uniformis tl-1.	the bacteriocin of bacteroides uniformis t1-1 had previously been reported to have a molecular weight of greater than or equal to 300 000. reexamination of the b. uniformis bacteriocin revealed that the bacteriocin was found in association with membrane vesicles which had been released by bleb formation from the outer surface of the b. uniformis cells. the bacteriocin could be released from whole cells or purified membrane vesicles by treatment with 6 m guanidine hydrochloride or 7 m urea and ha ...	1984	6713308
rapid glutamic acid decarboxylase test for identification of bacteroides and clostridium spp.	a rapid 4-h test for glutamic acid decarboxylase is described for the identification of certain anaerobic bacteria. the test substrate consisted of 1.0 g of l-glutamic acid, 0.3 ml of triton x-155, and 0.05 g of bromcresol green sodium salt in 1 liter of water. the substrate was dispensed in 0.5-ml amounts into test tubes, and a turbid suspension was made with the test organism. the test was then incubated aerobically at 35 degrees c for 4 h. the development of a blue color was considered positi ...	1984	6376535
taxonomy of anaerobes: present state of the art.	results of nucleic acid studies, which indicate relationships among strains and species more clearly than do usual phenotypic tests, have led to new bacteriologic nomenclature. some major changes in bacteroides include the recognition of three species (bacteroides melaninogenicus, bacteroides denticola, and bacteroides loescheii) formerly grouped in b. melaninogenicus subspecies melaninogenicus; two species (bacteroides intermedius with two closely related homology groups and bacteroides corpori ...	1984	6201990
[x-ray radioisotope diagnosis of tumor metastases of urogenital organs into the skeleton].		2015	5063119
amenorrhoea after the pill.		2016	5058752
emergency search for a bleeding diathesis: the internist as a coagulation consultant.		2016	5055705
[new morphological and functional aspects of the round window].		1972	5050012
[diagnosis and treatment of conductive hearing losses in childhood].		2012	5050011
[significance of alveolar ventilation irregularity in the development of external respiration insufficiency in chronic pneumonia].		2016	5039706
local and systemic effects of phenylalanine on intestinal transport of tyrosine and tryptophan.		1972	5037606
thyroid development in ducks.		1972	5028039
[methods of decomposing microbial cells for determination of their atp content].		1971	4999547
[organ and species specificity of leucine aminopeptidase from bovine lens].		1970	4991388
the uvea.		1967	4960637
[incorporation into bacillus subtilis ribosomes of ribonucleic acid synthesized under the influence of chloramphenicol in the presence of actinomycin d].		1965	4958012
evaluation of tests to determine the virulence of tubercle bacilli.		1966	4957795
[reconstructive tegumentary surgery of the lower limb].		2015	4949658
[reconstructive tegumentary surgery of the lower limb].		2015	4949658
[increased risk of cardiovascular complications with oral hypoglycemic agents?].		2016	4949515
a cellular deficit in the reconstitutive capacity of immune populations of lymphoid cells demonstrable in studies of delayed hypersensitivity in mice. evidence for thymus-bone marrow cell synergism.	a cell-transfer system was employed in the present work to investigate several characteristics of the capacity of immune and normal lymphoid cells to transfer the delayed response to methylated human serum albumin in lethally irradiated syngeneic recipients. spleen cells derived from donor mice immunized with goose erythrocytes were far less effective in transferring responsiveness when compared with equal numbers of normal cells. statistical analyses indicated a frequency of 1 reactive cell or ...	1971	4928817
[bacterial chemotypes in epidemiology].		2016	4928084
the crushed chest. cardiovascular trauma.		2015	4913632
the crushed chest. cardiovascular trauma.		2015	4913632
the induction of neurogenic hypercholesteremia.		1969	4893116
the induction of neurogenic hypercholesteremia.		1969	4893116
[pumonary transplantation. (preliminary experimental study on dogs)].		1968	4879732
device for linear gradient dehydration of specimens for electron microscopy.		2016	4873001