Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| an isolate of haemophilus haemolyticus produces a bacteriocin-like substance that inhibits the growth of nontypeable haemophilus influenzae. | nontypeable haemophilus influenzae (nthi) frequently colonises the upper respiratory tract and is an important cause of respiratory infections. resistance to antibiotics is an emerging trend in nthi and alternative prevention or treatment strategies are required. haemophilus haemolyticus is a common commensal occupying the same niche as nthi and, if able to produce substances that inhibit nthi growth, may have a role as a probiotic. in this study, ammonium sulphate extracts from broth culture of ... | 2017 | 28242259 |
| comparative genomic analysis of haemophilus haemolyticus and nontypeable haemophilus influenzae and a new testing scheme for their discrimination. | haemophilus haemolyticus has been recently discovered to have the potential to cause invasive disease. it is closely related to nontypeable haemophilus influenzae (nt h. influenzae). nt h. influenzae and h. haemolyticus are often misidentified because none of the existing tests targeting the known phenotypes of h. haemolyticus are able to specifically identify h. haemolyticus through comparative genomic analysis of h. haemolyticus and nt h. influenzae, we identified genes unique to h. haemolytic ... | 2016 | 27707939 |
| inability of haemophilus haemolyticus to invade respiratory epithelial cells in vitro. | 2016 | 27624822 | |
| duplex quantitative pcr assay for detection of haemophilus influenzae that distinguishes fucose- and protein d-negative strains. | we have developed a specific haemophilus influenzae quantitative pcr (qpcr) that also identifies fucose-negative and protein d-negative strains. analysis of 100 h. influenzae isolates, 28 haemophilus haemolyticus isolates, and 14 other bacterial species revealed 100% sensitivity (95% confidence interval [ci], 96% to 100%) and 100% specificity (95% ci, 92% to 100%) for this assay. the evaluation of 80 clinical specimens demonstrated a strong correlation between semiquantitative culture and the qp ... | 2016 | 27335148 |
| haemophilus haemolyticus interaction with host cells is different to nontypeable haemophilus influenzae and prevents nthi association with epithelial cells. | nontypeable haemophilus influenzae (nthi) is an opportunistic pathogen that resides in the upper respiratory tract and contributes to a significant burden of respiratory related diseases in children and adults. haemophilus haemolyticus is a respiratory tract commensal that can be misidentified as nthi due to high levels of genetic relatedness. there are reports of invasive disease from h. haemolyticus, which further blurs the species boundary with nthi. to investigate differences in pathogenicit ... | 2016 | 27242968 |
| comparative analyses of the lipooligosaccharides from nontypeable haemophilus influenzae and haemophilus haemolyticus show differences in sialic acid and phosphorylcholine modifications. | haemophilus haemolyticus and nontypeable haemophilus influenzae (nthi) are closely related upper airway commensal bacteria that are difficult to distinguish phenotypically. nthi causes upper and lower airway tract infections in individuals with compromised airways, while h. haemolyticus rarely causes such infections. the lipooligosaccharide (los) is an outer membrane component of both species and plays a role in nthi pathogenesis. in this study, comparative analyses of the los structures and cor ... | 2016 | 26729761 |
| a 7-deazaadenosylaziridine cofactor for sequence-specific labeling of dna by the dna cytosine-c5 methyltransferase m.hhai. | dna methyltransferases (mtases) catalyze the transfer of the activated methyl group of the cofactor s-adenosyl-l-methionine (adomet or sam) to the exocyclic amino groups of adenine or cytosine or the c5 ring atom of cytosine within specific dna sequences. the dna adenine-n6 mtase from thermus aquaticus (m.taqi) is also capable of coupling synthetic n-adenosylaziridine cofactor analogues to its target adenine within the double-stranded 5'-tcga-3' sequence. this m.taqi-mediated coupling reaction w ... | 2015 | 26610450 |
| identification of haemophilus haemolyticus in clinical samples and characterization of their mechanisms of antimicrobial resistance. | the objectives of this study were to establish the frequency of haemophilus haemolyticus in clinical samples, to determine the antimicrobial resistance rate and to identify the mechanisms of resistance to β-lactams and quinolones. | 2016 | 26472767 |
| respiratory bacterial culture from two sequential bronchoalveolar lavages of the same lobe in children with chronic cough. | identification of bacteria causing lower-airway infections is important to determine appropriate antimicrobial therapy. flexible bronchoscopy with bronchoalveolar lavage (bal) is used to obtain lower-airway specimens in young children. the first lavage (lavage-1) is typically used for bacterial culture. however, no studies in children have compared the detection of cultivable bacteria from sequential lavages of the same lobe. bal fluid was collected from two sequential lavages of the same lobe i ... | 2015 | 26399701 |
| complete deletion of the fucose operon in haemophilus influenzae is associated with a cluster in multilocus sequence analysis-based phylogenetic group ii related to haemophilus haemolyticus: implications for identification and typing. | nonhemolytic variants of haemophilus haemolyticus are difficult to differentiate from haemophilus influenzae despite a wide difference in pathogenic potential. a previous investigation characterized a challenging set of 60 clinical strains using multiple pcrs for marker genes and described strains that could not be unequivocally identified as either species. we have analyzed the same set of strains by multilocus sequence analysis (mlsa) and near-full-length 16s rrna gene sequencing. mlsa unambig ... | 2015 | 26378279 |
| genital carriage of the genus haemophilus in pregnancy: species distribution and antibiotic susceptibility. | recent reports have hypothesized that colonization of the maternal genital tract with non-capsulated haemophilus influenzae could result in neonatal invasive disease. in this study, genital carriage of the genus haemophilus was investigated in 510 pregnant women attending an italian hospital for routine controls. overall, vaginal carriage of the genus haemophilus was 9.0 % (46/510). a high colonization rate with haemophilus parainfluenzae (37/510, 7.3 %) was found; other species, such as haemoph ... | 2015 | 25976004 |
| interspecies transfer of the penicillin-binding protein 3-encoding gene ftsi between haemophilus influenzae and haemophilus haemolyticus can confer reduced susceptibility to β-lactam antimicrobial agents. | mutations in ftsi, encoding penicillin-binding protein 3, can cause decreased β-lactam susceptibility in haemophilus influenzae. sequencing of ftsi from clinical strains has indicated interspecies recombination of ftsi between h. influenzae and haemophilus haemolyticus. this study documented apparently unrestricted homologous recombination of ftsi between h. influenzae and h. haemolyticus in vitro. transfer of ftsi from resistant isolates conferred similar but not identical increases in the mics ... | 2015 | 25918135 |
| what the pediatrician should know about non-typeable haemophilus influenzae. | non-typeable haemophilus influenzae (nthi) live exclusively in the pharynges of humans and are increasingly recognized as pathogens that cause both localized infections of the respiratory tract (middle ear spaces, sinuses, and bronchi) and systemic infections such as bacteraemia and pneumonia. only one vaccine antigen of nthi, protein d, has been extensively studied in humans and its efficacy in preventing nthi otitis media is modest. recent genetic analyses reveal that nthi are closely related ... | 2015 | 25917803 |
| comparative profile of heme acquisition genes in disease-causing and colonizing nontypeable haemophilus influenzae and haemophilus haemolyticus. | nontypeable haemophilus influenzae (nthi) are gram-negative bacteria that colonize the human pharynx and can cause respiratory tract infections, such as acute otitis media (aom). since nthi require iron from their hosts for aerobic growth, the heme acquisition genes may play a significant role in avoiding host nutritional immunity and determining virulence. therefore, we employed a hybridization-based technique to compare the prevalence of five heme acquisition genes (hxua, hxub, hxuc, hemr, and ... | 2015 | 25903577 |
| difficult identification of haemophilus influenzae, a typical cause of upper respiratory tract infections, in the microbiological diagnostic routine. | haemophilus influenzae is a key pathogen of upper respiratory tract infections. its reliable discrimination from nonpathogenic haemophilus spp. is necessary because merely colonizing bacteria are frequent at primarily unsterile sites. due to close phylogenetic relationship, it is not easy to discriminate h. influenzae from the colonizer haemophilus haemolyticus. the frequency of h. haemolyticus isolations depends on factors like sampling site, patient condition, and geographic region. biochemica ... | 2015 | 25883794 |
| identifying haemophilus haemolyticus and haemophilus influenzae by sybr green real-time pcr. | sybr green real time pcr assays for protein d (hpd), fuculose kinase (fuck) and [cu, zn]-superoxide dismutase (sodc) were designed for use in an algorithm for the identification of haemophilus influenzae and h. haemolyticus. when tested on 127 h. influenzae and 60 h. haemolyticus all isolates were identified correctly. | 2015 | 25753676 |
| relationship between clinical site of isolation and ability to form biofilms in vitro in nontypeable haemophilus influenzae. | nontypeable haemophilus influenzae (nthi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. there is some debate as to whether or not nthi produces biofilms and, if so, whether or not this is relevant to pathogenesis. although many studies have examined the association between in vitro biofilm formation and isolates from a specific infection type, few have made comparisons from isolates from a bro ... | 2015 | 25706230 |
| molecular tools for differentiation of non-typeable haemophilus influenzae from haemophilus haemolyticus. | non-typeable haemophilus influenzae (nthi) and haemophilus haemolyticus are closely related bacteria that reside in the upper respiratory tract. nthi is associated with respiratory tract infections that frequently result in antibiotic prescription whilst h. haemolyticus is rarely associated with disease. nthi and h. haemolyticus can be indistinguishable by traditional culture methods and molecular differentiation has proven difficult. this current review chronologically summarizes the molecular ... | 2014 | 25520712 |
| haemophilus haemolyticus is infrequently misidentified as haemophilus influenzae in diagnostic specimens in australia. | the commensal haemophilus haemolyticus is difficult to differentiate from the respiratory pathogen haemophilus influenzae using phenotypic tests. in a study that used molecular tests to retrospectively identify 447 phenotypically identified h. influenzae isolates from diagnostic specimens in australia, only 7 (1.5%) h. haemolyticus were identified. | 2014 | 25266675 |
| difficulties in species identification within the genus haemophilus - a pilot study addressing a significant problem for routine diagnostics. | diagnostic misidentifications of commensalic haemophilus haemolyticus as pathogenic haemophilus influenzae are frequent. this pilot study evaluates whether isolations of h. haemolyticus are frequent enough in germany to cause a relevant diagnostic problem, considering the fact that even h. influenzae is a mere colonizer in about 30% of isolations. in microbiological laboratories of two hospitals located in northern and southern germany, the distribution of haemophilus spp. was analyzed during a ... | 2014 | 24883195 |
| classification, identification, and clinical significance of haemophilus and aggregatibacter species with host specificity for humans. | the aim of this review is to provide a comprehensive update on the current classification and identification of haemophilus and aggregatibacter species with exclusive or predominant host specificity for humans. haemophilus influenzae and some of the other haemophilus species are commonly encountered in the clinical microbiology laboratory and demonstrate a wide range of pathogenicity, from life-threatening invasive disease to respiratory infections to a nonpathogenic, commensal lifestyle. new sp ... | 2014 | 24696434 |
| design, synthesis and biological evaluation of 4-amino-n- (4-aminophenyl)benzamide analogues of quinoline-based sgi-1027 as inhibitors of dna methylation. | quinoline derivative sgi-1027 (n-(4-(2-amino-6-methylpyrimidin-4-ylamino)phenyl)-4-(quinolin-4-ylamino)benzamide) was first described in 2009 as a potent inhibitor of dna methyltransferase (dnmt) 1, 3a and 3b. based on molecular modeling studies, performed using the crystal structure of haemophilus haemolyticus cytosine-5 dna methyltransferase (mhhai c5 dnmt), which suggested that the quinoline and the aminopyridimine moieties of sgi-1027 are important for interaction with the substrates and pro ... | 2014 | 24678024 |
| role of inter-species recombination of the ftsi gene in the dissemination of altered penicillin-binding-protein-3-mediated resistance in haemophilus influenzae and haemophilus haemolyticus. | to screen the ftsi gene sequences obtained from clinical isolates of non-typeable haemophilus influenzae (nthi) and haemophilus haemolyticus for the presence of mosaic ftsi gene structures, and to evaluate the role of inter-species recombination of the ftsi gene in the formation and distribution of resistant ftsi genes. | 2014 | 24562614 |
| unidentified intralesional and intracellular coccoid microorganism discovered in the young man with a diffuse erosive gastroduodenitis and multiple superficial ulcerations. | a helicobacter pylori-negative young japanese man with dyspeptic symptoms suffered from a diffuse erosive gastroduodenitis and multiple superficial ulcerations. histology and electron microscopic examinations on the biopsy specimens revealed the presence of multiple unidentified intralesional and intracellular coccoid microorganisms in the pathological gastroduodenal mucosa. microaerophilic and anaerobic gram-negative coccoid and filamentobacillary bacteria were cultured from the gastric aspirat ... | 2014 | 24495976 |
| a pcr-high-resolution melt assay for rapid differentiation of nontypeable haemophilus influenzae and haemophilus haemolyticus. | we have developed a pcr-high-resolution melt (pcr-hrm) assay to discriminate nontypeable haemophilus influenzae (nthi) colonies from haemophilus haemolyticus. this method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. pcr-hrm is ideal for high-throughput screening for nthi surveillance and clinical trials. | 2014 | 24478508 |
| microbial diversity and dynamics during the production of may bryndza cheese. | diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ... | 2014 | 24291178 |
| identification of haemophilus influenzae and haemophilus haemolyticus by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | generally accepted laboratory methods that have been used for decades do not reliably distinguish between h. influenzae and h. haemolyticus isolates. h. haemolyticus strains are often incorrectly identified as nontypeable haemophilus influenzae (nthi). to distinguish h. influenzae from h. haemolyticus we have created a new database on the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) bio-typer 2 and compared the results with routine determination of ... | 2014 | 24019162 |
| pcr screening for the n526k substitution in isolates of haemophilus influenzae and haemophilus haemolyticus. | firstly, to evaluate the current pbp3-s primers of hasegawa et al. (microb drug resist 2003; 9: 39-46) and develop new primers for the amplification of n526 in isolates of haemophilus haemolyticus. secondly, to develop a new pcr assay for the detection (by amplification) of the n526k substitution, encoded by either the aaa or aag single nucleotide polymorphism (snp) at position 1576-1578 of the ftsi gene, in isolates of both haemophilus influenzae and h. haemolyticus. | 2013 | 23645583 |
| microbiological diagnosis and antibiotic therapy in patients with community-acquired pneumonia and acute copd exacerbation in daily clinical practice: comparison to current guidelines. | the aim of this secondary analysis was to evaluate current microbiological approaches, microbiology, and antibiotic therapy in patients with community-acquired pneumonia (cap) and acute exacerbation of chronic obstructive pulmonary disease (aecopd) in clinical practice and to compare them with current international guidelines. | 2013 | 23564195 |
| maldi-tof ms distinctly differentiates nontypable haemophilus influenzae from haemophilus haemolyticus. | nontypable haemophilus influenzae (nthi) and haemophilus haemolyticus exhibit different pathogenicities, but to date, there remains no definitive and reliable strategy for differentiating these strains. in this study, we evaluated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) as a potential method for differentiating nthi and h. haemolyticus. the phylogenetic analysis of concatenated 16s rrna and recombinase a (reca) gene sequences, outer membrane pr ... | 2013 | 23457514 |
| prevalence and mechanisms of β-lactam resistance in haemophilus haemolyticus. | to compare the phenotypic and genotypic β-lactam resistance profiles of non-typeable haemophilus influenzae (nthi) and the closely phylogenetically related haemophilus haemolyticus. | 2013 | 23315478 |
| haemophilus haemolyticus isolates causing clinical disease. | we report seven cases of haemophilus haemolyticus invasive disease detected in the united states, which were previously misidentified as nontypeable haemophilus influenzae. all cases had different symptoms and presentations. our study suggests that a testing scheme that includes reliable pcr assays and standard microbiological methods should be used in order to improve h. haemolyticus identification. | 2012 | 22573587 |
| culture and pcr detection of haemophilus influenzae and haemophilus haemolyticus in australian indigenous children with bronchiectasis. | a pcr for protein d (hpd#3) was used to differentiate nontypeable haemophilus influenzae (nthi) from haemophilus haemolyticus. while 90% of nasopharyngeal specimens and 100% of lower-airway specimens from 84 indigenous australian children with bronchiectasis had phenotypic nthi isolates confirmed as h. influenzae, only 39% of oropharyngeal specimens with phenotypic nthi had h. influenzae. the nasopharynx is therefore the preferred site for nthi colonization studies, and nthi is confirmed as an i ... | 2012 | 22553240 |
| molecular surveillance of true nontypeable haemophilus influenzae: an evaluation of pcr screening assays. | unambiguous identification of nontypeable haemophilus influenzae (nthi) is not possible by conventional microbiology. molecular characterisation of phenotypically defined nthi isolates suggests that up to 40% are haemophilus haemolyticus (hh); however, the genetic similarity of nthi and hh limits the power of simple molecular techniques such as pcr for species discrimination. | 2012 | 22470516 |
| an invasive haemophilus haemolyticus isolate. | 2012 | 22301029 | |
| evaluation of new biomarker genes for differentiating haemophilus influenzae from haemophilus haemolyticus. | pcr detecting the protein d (hpd) and fuculose kinase (fuck) genes showed high sensitivity and specificity for identifying haemophilus influenzae and differentiating it from h. haemolyticus. phylogenetic analysis using the 16s rrna gene demonstrated two distinct groups for h. influenzae and h. haemolyticus. | 2012 | 22301020 |
| genome sequences for five strains of the emerging pathogen haemophilus haemolyticus. | we report the first whole-genome sequences for five strains, two carried and three pathogenic, of the emerging pathogen haemophilus haemolyticus. preliminary analyses indicate that these genome sequences encode markers that distinguish h. haemolyticus from its closest haemophilus relatives and provide clues to the identity of its virulence factors. | 2011 | 21952546 |
| medical microbiology | the genus haemophilus includes a number of species that cause a wide variety of infections but share a common morphology and a requirement for blood-derived factors during growth that has given the genus its name. haemophilus influenzae, the major pathogen, can be separated into encapsulated or typable strains, of which there are seven types (a through f including e') based on the antigenic structure of the capsular polysaccharide, and unencapsulated or nontypable strains. type b h influenzae is ... | 1996 | 21413340 |
| prevalence of genetic differences in phosphorylcholine expression between nontypeable haemophilus influenzae and haemophilus haemolyticus. | although non-typeable (nt) haemophilus influenzae and haemophilus haemolyticus are closely related human commensals, h. haemolyticus is non-pathogenic while nt h. influenzae is an important cause of respiratory tract infections. phase-variable phosphorylcholine (chop) modification of lipooligosaccharide (los) is a nt h. influenzae virulence factor that, paradoxically, may also promote complement activation by binding c-reactive protein (crp). crp is known to bind more to chop positioned distally ... | 2010 | 21073698 |
| haemophilus influenzae outer membrane protein p6 molecular characterization may not differentiate all strains of h. influenzae from h. haemolyticus. | distinguishing nontypeable haemophilus influenzae and haemophilus haemolyticus isolates by outer membrane protein (omp) p6 gene sequencing is complicated by sequence variants in isolates. further testing using rapid nh and multilocus sequence analysis may not help identify some isolates. translated omp p6 gene sequences are not conserved among all isolates presumed to be h. influenzae. | 2010 | 20686092 |
| nasopharyngeal carriage of haemophilus haemolyticus in otitis-prone and healthy children. | haemophilus haemolyticus is often incorrectly categorized as nontypeable haemophilus influenzae (nthi) upon culture. pcr analyses of 266 nthi-like nasopharyngeal isolates from children with and without recurrent acute otitis media (raom) revealed that 11.7% were h. haemolyticus and 9.4% gave equivocal results. children with raom were more likely to carry h. haemolyticus. | 2010 | 20463165 |
| prevalence of haemophilus influenzae type b genetic islands among clinical and commensal h. influenzae and h. haemolyticus isolates. | five genetic islands (higi) found in haemophilus influenzae type b strain eagan were used as hybridization probes on type b, haemophilus haemolyticus, and nontypeable h. influenzae (nthi) isolates. higi2 and higi7 were significantly more prevalent in nthi isolates from children with otitis media than in those from the throats of healthy children. | 2010 | 20463164 |
| haemophilus influenzae and haemophilus haemolyticus in tonsillar cultures of adults with acute pharyngotonsillitis. | the aim of this study was to evaluate the clinical implication of haemophilus haemolyticus, one of the closest relative of haemophilus influenzae, on acute pharyngotonsillitis. | 2010 | 20392581 |
| antimicrobial characterisation of cem-101 activity against respiratory tract pathogens, including multidrug-resistant pneumococcal serogroup 19a isolates. | cem-101 is a novel fluorinated macrolide-ketolide with potent activity against bacterial pathogens that are susceptible or resistant to other macrolide-lincosamide-streptogramin b (mls(b))-ketolide agents. cem-101 is being developed for oral and parenteral use in moderate to moderately severe community-acquired bacterial pneumonia. the objective of this study was to assess the activity of cem-101 and comparators against contemporary respiratory tract infection (rti) isolates. a worldwide sample ... | 2010 | 20211548 |
| prevalence of the sodc gene in nontypeable haemophilus influenzae and haemophilus haemolyticus by microarray-based hybridization. | the sodc gene has been reported to be a useful marker for differentiating nontypeable (nt) haemophilus influenzae from haemophilus haemolyticus in respiratory-tract samples, but discrepancies exist as to the prevalence of sodc in nt h. influenzae. therefore, we used a microarray-based, "library-on-a-slide" method to differentiate the species and found that 21 of 169 (12.4%) nt h. influenzae strains and all 110 (100%) h. haemolyticus strains possessed the sodc gene. multilocus sequence analysis c ... | 2010 | 20042621 |
| a growth inhibitory substance for the influenza group of organisms in the blood of various animal species : the use of the blood of various animals as a selective medium for the detection of hemolytic streptococci in throat cultures. | 1. 5 per cent sheep blood agar is a selective medium for beta hemolytic streptococci in throat cultures since sheep blood inhibits the growth of bacillus x (h. hemolyticus) and b. parainfluenzae hemolyticus. the growth of h. influenzae is also inhibited by sheep blood. 2. the inhibitory action of sheep blood resides in the erythrocytes and is thermolabile. disruption of the cell by laking has no effect upon the inhibitor. 3. the bloods of animals closely related to the sheep, such as the goat an ... | 1938 | 19870730 |
| studies in the common cold : i. observations of the normal bacterial flora of nose and throat with variations occurring during colds. | 1. the normal bacterial flora of the nose and throat of thirteen individuals has been studied over periods ranging from 5 to 9 months. 2. observations have been made of qualitative and quantitative changes in the flora occurring in the course of colds and of throat infections appearing in the group. 3. the normal basic nasal flora includes staphylococcus albus, diphtheroids, and for certain individuals staphylococcus aureus and citreus. occasional transient bacteria are gram-negative cocci and n ... | 1926 | 19869133 |
| a comparative study of the biological characters and pathogenesis of bacillus x (sternberg), bacillus icteroides (sanarelli), and the hog-cholera bacillus (salmon and smith). | 1. bacillus x (sternberg) belongs to the colon group. 2. bacillus icteroides (sanarelli) is a member of the hog-cholera group. 3. the various channels of infection, the duration of the disease and the gross and microscopical lesions in mice, guinea-pigs and rabbits are the same for bacillus icteroides and the hog-cholera bacillus. 4. the clinical symptoms and the lesions observed in dogs inoculated intravenously with bacillus icteroides, are reproduced in these animals by infection with the hog- ... | 1900 | 19866945 |
| detection of cryptic genospecies misidentified as haemophilus influenzae in routine clinical samples by assessment of marker genes fuck, hap, and sodc. | clinical isolates of haemophilus influenzae were assessed for the presence of fuck, hap, and sodc by hybridization with gene-specific probes, and isolates diverging from the expected h. influenzae genotype were characterized by phenotype and 16s rrna gene sequencing. two of 480 isolates were finally classified as variant strains ("nonhemolytic haemophilus haemolyticus"). | 2009 | 19535530 |
| delineation of the species haemophilus influenzae by phenotype, multilocus sequence phylogeny, and detection of marker genes. | to obtain more information on the much-debated definition of prokaryotic species, we investigated the borders of haemophilus influenzae by comparative analysis of h. influenzae reference strains with closely related bacteria including strains assigned to haemophilus haemolyticus, cryptic genospecies biotype iv, and the never formally validated species "haemophilus intermedius". multilocus sequence phylogeny based on six housekeeping genes separated a cluster encompassing the type and the referen ... | 2009 | 19060144 |
| relationships of nontypeable haemophilus influenzae strains to hemolytic and nonhemolytic haemophilus haemolyticus strains. | haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while h. haemolyticus, the closest phylogenetic relative of h. influenzae, is arguably a strict pharyngeal commensal. a hemolytic phenotype has historically differentiated h. haemolyticus from h. influenzae, but the recent recognition of significant nonhemolytic h. haemolyticus colonization has decreased this trait's resolvability. given this and the potential of recombination between the species, we examined t ... | 2008 | 18039799 |
| pharyngeal colonization dynamics of haemophilus influenzae and haemophilus haemolyticus in healthy adult carriers. | haemophilus influenzae is an important cause of respiratory infections, including acute otitis media, sinusitis, and chronic bronchitis, which are preceded by asymptomatic h. influenzae colonization of the human pharynx. the aim of this study was to describe the dynamics of pharyngeal colonization by h. influenzae and an intimately related species, haemophilus haemolyticus, in healthy adults. throat specimens from four healthy adult carriers were screened for haemophilus species; 860 isolates we ... | 2007 | 17687018 |
| airway bacterial concentrations and exacerbations of chronic obstructive pulmonary disease. | increased bacterial concentration (load) in the lower airways and new bacterial strain acquisition have been posited as mechanisms for chronic obstructive pulmonary disease (copd) exacerbations. bacterial concentrations are higher during exacerbation than during stable disease; however, these studies are cross sectional and devoid of strain typing. | 2007 | 17478618 |
| horizontal gene transfer of ftsi, encoding penicillin-binding protein 3, in haemophilus influenzae. | horizontal gene transfer has been identified in only a small number of genes in haemophilus influenzae, an organism which is naturally competent for transformation. this report provides evidence for the genetic transfer of the ftsi gene, which encodes penicillin-binding protein 3, in h. influenzae. mosaic structures of the ftsi gene were found in several clinical isolates of h. influenzae. to identify the origin of the mosaic sequence, complete sequences of the corresponding gene from seven type ... | 2007 | 17325223 |
| haemophilus haemolyticus: a human respiratory tract commensal to be distinguished from haemophilus influenzae. | haemophilus influenzae is a common pathogen in adults with chronic obstructive pulmonary disease (copd). in a prospective study, selected isolates of apparent h. influenzae had an altered phenotype. we tested the hypothesis that these variant strains were genetically different from typical h. influenzae. | 2007 | 17152011 |
| presence of copper- and zinc-containing superoxide dismutase in commensal haemophilus haemolyticus isolates can be used as a marker to discriminate them from nontypeable h. influenzae isolates. | respiratory isolates of haemophilus haemolyticus are regularly misclassified as nontypeable (nt) haemophilus influenzae due to an aberrant hemolytic reaction on blood agar, with implications for treatment. the presence of sodc or its cognate protein, copper-zinc superoxide dismutase, can distinguish respiratory isolates of h. haemolyticus from nt h. influenzae with 100% accuracy. | 2006 | 16988021 |
| a classification of the hemolytic bacteria of the genus haemophilus: haemophilus haemolyticus bergey et al. and haemophilus parahaemolyticus nov spec. | 1953 | 13069455 | |
| structural basis of icf-causing mutations in the methyltransferase domain of dnmt3b. | mutations in the gene encoding for a de novo methyltransferase, dnmt3b, lead to an autosomal recessive immunodeficiency, centromeric instability and facial anomalies (icf) syndrome. to analyse the protein structure and consequences of icf-causing mutations, we modelled the structure of the dnmt3b methyltransferase domain based on haemophilus haemolyticus protein in complex with the cofactor adomet and the target dna sequence. the structural model has a two-subdomain fold where the dna-binding re ... | 2002 | 12601140 |
| zebularine: a novel dna methylation inhibitor that forms a covalent complex with dna methyltransferases. | mechanism-based inhibitors of enzymes, which mimic reactive intermediates in the reaction pathway, have been deployed extensively in the analysis of metabolic pathways and as candidate drugs. the inhibition of cytosine-[c5]-specific dna methyltransferases (c5 mtases) by oligodeoxynucleotides containing 5-azadeoxycytidine (azadc) and 5-fluorodeoxycytidine (fdc) provides a well-documented example of mechanism-based inhibition of enzymes central to nucleic acid metabolism. here, we describe the int ... | 2002 | 12206775 |
| functional roles of the conserved aromatic amino acid residues at position 108 (motif iv) and position 196 (motif viii) in base flipping and catalysis by the n6-adenine dna methyltransferase from thermus aquaticus. | the dna methyltransferase (mtase) from thermus aquaticus (m.taqi) catalyzes the transfer of the activated methyl group of s-adenosyl-l-methionine to the n6 position of adenine within the double-stranded dna sequence 5'-tcga-3'. to achieve catalysis m.taqi flips the target adenine out of the dna helix. on the basis of the three-dimensional structure of m.taqi in complex with the cofactor and its structural homology to the c5-cytosine dna mtase from haemophilus haemolyticus, tyr 108 and phe 196 we ... | 1999 | 9931007 |
| genetic characterization of a cryptic genospecies of haemophilus causing urogenital and neonatal infections. | in recent years, reports originating from several areas of the world have identified biotype iv strains of haemophilus influenzae as a cause of serious urogenital, neonatal, and mother-infant infections. preliminary analysis of a sample of biotype iv isolates found evidence for a cryptic genospecies of haemophilus (r. quentin, a. goudeau, r. j. wallace, jr., a. l. smith, r. k. selander, and j. m. musser. j. gen. microbiol. 136:1203-1209, 1990). eighteen biotype iv strains assigned to the cryptic ... | 1993 | 8099082 |
| binding of human hemoglobin by haemophilus influenzae. | binding of biotinylated human hemoglobin to haemophilus influenzae was detected when organisms were grown in heme-deplete, but not heme-replete, conditions. hemoglobin binding was completely inhibited by a 100-fold excess of unlabelled human hemoglobin or human hemoglobin complexed with human haptoglobin. binding was only partially inhibited by rat hemoglobin, bovine hemoglobin, human globin, and bovine globin, and not at all by heme, human serum albumin, bovine serum albumin, human transferrin, ... | 1994 | 8020748 |
| relative proportions of haemophilus species in the throat of healthy children and adults. | to determine normal proportions of pharyngeal haemophilus species, qualitative and quantitative mapping of the species in the pharynx of ten healthy children and ten healthy adults was carried out using a selective and a non-selective medium. haemophilus organisms were present in all samples, comprising approximately 10% of the total cultivable flora (range 0.6-36.9%). haemophilus parainfluenzae was a member of the normal flora throughout life, constituting 74% of pharyngeal haemophilus organism ... | 1984 | 6332018 |
| the nucleotide sequence of the hhaii restriction and modification genes from haemophilus haemolyticus. | we have determined the nucleotide sequence of a cloned 1710-bp segment of haemophilus haemolyticus dna which contains the hhaii restriction (r) and modification (m) genes. the m gene is 513 bp in length and the rgene is 681 bp in length. both are in the same reading frame, being separated by a 21-bp region. a ribosome-binding site is identified in front of each gene, but no haemophilus promoter is apparent on the cloned fragment. transcription originates from a plasmid promotor and proceeds in t ... | 1983 | 6315538 |
| the nucleotide sequence of is5 from escherichia coli. | a 3-kb fragment of haemophilus haemolyticus dna which carries the hhaii restriction (r) and modification (m) genes has been cloned into the psti site of pbr322 (mann et al., 1978). when propagated in escherichia coli, it was observed that spontaneous insertions of is5 inactivated the restriction gene, producing r- mutants at a frequency of 10(-6). electron microscopy, restriction-site mapping and sequence analysis of two r- plasmids have demonstrated the presence of is5 at a single target site i ... | 1981 | 6269959 |
| fimbriation of haemophilus species isolated from the respiratory tract of adults. | twenty-two clinical isolates of haemophilus species were studied within two passages of their original isolation for the presence of fimbriae by negative-staining electron microscopy. six isolates were identified as fimbriated, including three strains of nontypable haemophilus influenzae, one strain of haemophilus parainfluenzae, and two strains of haemophilus haemolyticus. in fresh isolates of fimbriated strains of nontypable h. influenzae, approximately 40%-50% of cells had fimbriae; after fiv ... | 1984 | 6146651 |
| hhai methylase and restriction endonuclease as probes for b to z dna conformational changes in d(gcgc) sequences. | the capacity of the modification methylase (mhhai) and restriction endonuclease (hhai) form haemophilus haemolyticus to methylate and cleave, respectively, recognition sites which are in right-handed b or left-handed z structures was determined in vitro. plasmids containing tracts of (dc-dg) as well as numerous individual d(gcgc) sites distributed around the vector were studied. negative supercoiling was used to convert the (dc-dg) tracts (approximately 30 bp in length) from a right-handed to a ... | 1984 | 6093048 |
| [sarcomatoid stroma epitheliomas of the oropharynx and larynx]. | 2016 | 5078883 | |
| unusual features in familial asphyxiating thoracic dysplasia (jeune's disease). | 2016 | 5054317 | |
| health maintenance organization. | 2016 | 4936249 | |
| blood flow through different regions of long bone measured by a bone-seeking radioisotopic method. | 1971 | 4923134 | |
| renal allograft rejection simulated by arterial stenosis. | 2016 | 4919685 | |
| long-term treatment of alcoholism with amitriptyline and emylcamate. a double-blind evaluation. | 2016 | 4860508 | |
| vertebrate collagen heterogeneity: a brief summary. | 2016 | 4831113 | |
| [clinical use of anti-aggregative agents]. | 2016 | 4771351 | |
| change in transcription of repetitive dna sequences in n-methyl-n'-nitro-n-nitrosoguanidine-transformed hamster cells. | 2015 | 4735236 | |
| change in transcription of repetitive dna sequences in n-methyl-n'-nitro-n-nitrosoguanidine-transformed hamster cells. | 2015 | 4735236 | |
| frequency of hepatitis b antigen in blood donors. | routine testing of all blood donations for hepatitis b antigen by counter-immunoelectrophoresis was started in april 1971. the frequency of hbag carriers among apparently healthy volunteer blood donors in the toronto centre of the canadian red cross blood transfusion service was 15 in 10,000 with a male:female ratio of 6:1. excluding penitentiary inmates, the highest incidence was among young, male, non-anglo-saxon city dwellers.a system of notification of hbag-positive donors was developed, thr ... | 2015 | 4689555 |
| [perirenal steatonecrosis or retroperitoneal necrosing panniculitis (apropos of a case)]. | 2015 | 4652104 | |
| isolation of a new rna polymerase-binding protein from sporulating bacillus subtilis. | rna polymerase was precipitated from extracts of radioactively labeled vegetative and sporulating bacillus subtilis with antiserum prepared against vegetative core polymerase. the precipitates were solubilized and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. antiserum added to an extract of vegetative b. subtilis precipitated only the known subunits of core rna polymerase, but antiserum added to an extract of sporulating cells precipitated a new polypeptide of 70,000 da ... | 1973 | 4631355 |
| [studies on murine leprosy bacillus. x. rapid growing mycobacteria isolated from experimental murine leprosy mice (author's transl)]. | 1974 | 4617060 | |
| effects of oxidized spermine and acrolein on the transforming activity of t4 dna. | 1972 | 4552901 | |
| effects of oxidized spermine and acrolein on the transforming activity of t4 dna. | 1972 | 4552901 | |
| transposed great arteries combined with totally anomalous pulmonary veins. a report of a successful correction. | 2015 | 4551764 | |
| suppressor cells in the rabbit appendix. | 1974 | 4547314 | |
| [medical ethics with respect to the medical care structure]. | 2015 | 4540933 | |
| legislating education in dentistry. | 2015 | 4520738 | |
| facial neuralgia caused by unrecognized foreign bodies: report of case. | 2015 | 4509705 | |
| a perspective on prophylaxis of dental caries by topical fluoride. | 2014 | 4506529 | |
| a nursing research workshop. | 2015 | 4493005 | |
| conversion of human hexosaminidase a to hexosaminidase "b" by crude vibrio cholerae neuraminidase preparations: merthiolate is the active factor. | 1974 | 4473220 | |
| purification of large crosslinked peptides from insoluble calf bone and skin collagens by hydroxyapatite chromatography. | 1974 | 4473211 | |
| [comparative study of the therapeutic results and of clinical and psychosocial data from a medico-social guidance center for alcoholics]. | 2015 | 4468707 | |
| studies on murine leprosy bacillus. x. studies on an atypical mycobacteria isolated from experimental murine leprosy mice. | 1974 | 4449161 | |
| [characteristics of bioenergetic processes in erythrocytes of patients with progressive muscular dystrophy]. | 2015 | 4446546 | |
| [cervical spinal lesions]. | 2015 | 4425335 | |
| clinical grand reounds: the many facets of hemophilia. | 2014 | 4406153 | |
| clinical grand reounds: the many facets of hemophilia. | 2014 | 4406153 | |
| preparation of glutamate dehydrogenase from micrococcus aerogenes. | 1970 | 4397848 | |
| mechanism of adrenaline-induced lipolysis in adipose tissue. | 1974 | 4363679 |