Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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soil properties and microbial ecology of a paddy field after repeated applications of domestic and industrial sewage sludges. | the effects of repeated application of two types of sewage sludge, domestic and industrial (petrochemical, pss) sludges, into paddy fields over a 5-year period on the soil properties and microbial ecology were studied and compared with conventional npk fertilizer application. soil organic matter and total nitrogen contents were significantly higher in the two sludge treatments than that in fertilized plots after 5 years. soil concentrations of potentially toxic metals were low after 5 years of b ... | 2017 | 28194679 |
exposure of luminous marine bacteria to low-dose gamma-radiation. | the study addresses biological effects of low-dose gamma-radiation. radioactive (137)cs-containing particles were used as model sources of gamma-radiation. luminous marine bacterium photobacterium phosphoreum was used as a bioassay with the bioluminescent intensity as the physiological parameter tested. to investigate the sensitivity of the bacteria to the low-dose gamma-radiation exposure (≤250 mgy), the irradiation conditions were varied as follows: bioluminescence intensity was measured at 5, ... | 2017 | 28086187 |
acute toxicity reduction and toxicity identification in pigment-contaminated wastewater during anaerobic-anoxic-oxic (a/a/o) treatment process. | in china, a considerable part of industrial wastewater effluents are discharged into the municipal wastewater treatment plants (wwtps) after pretreatment in their own wastewater treatment plants. even though the industrial effluents meet the professional emission standards, many micro-pollutants still remained, and they could be resistant in the municipal wwtps with conventional activated sludge process. pigment wastewater was chosen in this study, and the acute toxicity reduction and identifica ... | 2017 | 27919537 |
a chemometrics approach applied to fourier transform infrared spectroscopy (ftir) for monitoring the spoilage of fresh salmon (salmo salar) stored under modified atmospheres. | the aim of this work was to investigate the potential of fourier transform infrared spectroscopy (ftir) to detect and predict the bacterial load of salmon fillets (salmo salar) stored at 3, 8 and 30°c under three packaging conditions: air packaging (ap) and two modified atmospheres constituted by a mixture of 50%n2/40%co2/10%o2 with lemon juice (mapl) and without lemon juice (map). fresh salmon samples were periodically examined for total viable counts (tvc), specific spoilage organisms (sso) co ... | 2017 | 27838518 |
acute toxicity assessment of explosive-contaminated soil extracting solution by luminescent bacteria assays. | explosive-contaminated soil is harmful to people's health and the local ecosystem. the acute toxicity of its extracting solution was tested by bacterial luminescence assay using three kinds of luminescent bacteria to characterize the toxicity of the soil. an orthogonal test l 16 (4(5)) was designed to optimize the soil extracting conditions. the optimum extracting conditions were obtained when the ultrasonic extraction time, ultrasonic extraction temperature, and the extraction repeat times were ... | 2016 | 27566156 |
immunological relationships of glutamine synthetases from marine and terrestrial enterobacteria. | glutamine synthetase (gs) frombeneckea alginolytica strain 90 was purified to homogeneity as indicated by sodium dodecyl sulface polyacrylamide gel electrophoresis. the purified enzyme was injected into rabbits, and the antiserum, as well as previously prepared antiserum against the gs fromescherichia coli, was used in ouchterlony double diffusion experiments. from the results of these studies, the marine and terrestrial enterobacteria could be separated into five major groups consisting of (i)b ... | 1980 | 27520760 |
[comparative sensitivity of the luminescent photobacterium phosphoreum, escherichia coli, and bacillus subtilis strains to toxic effects of carbon-based nanomaterials and metal nanoparticles]. | a comparative analysis of the four commercially available and laboratory luminescent sensor strains to the toxic effect of 10 carbon-based nanomatherials (cbns) and 10 metal nanoparticles (mnps) was carried out in this study. the bioluminescence inhibition assays with marine photobacterium phosphoreum and recombinant escherichia coli strains were varied in minimal toxic concentrations and ec50 values but led to well correlated biotoxicity evaluation for the most active compounds were ranked as c ... | 2016 | 27476206 |
eco-toxicological bioassay of atmospheric fine particulate matter (pm2.5) with photobacterium phosphoreum t3. | a bioluminescent bacterium, photobacterium phosphoreum t3 (ppt3), was used as a bio-indicator for the atmospheric fine particulate matter (pm2.5) to determine the eco-toxicity of pm2.5. the pm2.5 contains toxic chemicals, which reduce light output. the pm2.5 samples were collected in the period from march 2014 to january 2015 in nanjing and analyzed for the chemical composition versus their eco-toxicity. the eco-toxicological responses of each toxicant were detected in pm2.5 samples with ppt3. t ... | 2016 | 27467023 |
oxidative degradation of triclosan by potassium permanganate: kinetics, degradation products, reaction mechanism, and toxicity evaluation. | in this study, we systematically investigated the potential applicability of potassium permanganate for removal of triclosan (tcs) in water treatment. a series of kinetic experiments were carried out to study the influence of various factors, including the ph, oxidant doses, temperature, and presence of typical anions (cl(-), so4(2-), no3(-)), humic acid (ha), and fulvic acid (fa) on triclosan removal. the optimal reaction conditions were: ph = 8.0, [tcs]0:[kmno4]0 = 1:2.5, and t = 25 °c, where ... | 2016 | 27459151 |
joint toxicity of two phthalates with waterborne copper to daphnia magna and photobacterium phosphoreum. | di-n-butyl phthalate (dbp) and di-2-ethylhexyl phthalate (dehp) are two widely used phthalates, while cu(ii) is a common valence state of copper. they have been ubiquitously detected in the aquatic environment, but information on their joint toxicity to aquatic organisms is scarce. in this study, we evaluated the combined effects of copper and these two phthalates to daphnia magna and photobacterium phosphoreum by quantifying the acute toxicity expressed by the ec50 (the concentration causing 50 ... | 2016 | 27385371 |
environmental quality in sediments of cadiz and algeciras bays based on a weight of evidence approach (southern spanish coast). | this research applies an integrated sediment quality assessment method using a weight of evidence approach to cadiz and algeciras bays (southern spain). the method is composed of several analyses (particle size profile, aqua regia extractable metals, acid labile metals, total organic carbon, toxicity bioassay with photobacterium phosphoreum and macrobenthic community alteration). the proposed method provides a single result, the environmental degradation index (edi). edi defined samples as low d ... | 2016 | 27371957 |
paraphotobacterium marinum gen. nov., sp. nov., a member of the family vibrionaceae, isolated from surface seawater. | a novel gram-stain-negative bacterium, named strain nscs20n07dt, was isolated from surface seawater of the south china sea. cells of this strain contained poly-β-hydroxybutyrate granules. growth was observed at 15-35 °c with optimum of 30 °c, at a salinity range of 1-6 % (w/v) nacl with optimum of 3 % and at ph 5-8 with optimum of ph 5. the full-length 16s rrna gene sequence of strain nscs20n07dt showed highest similarity to photobacterium iliopiscarium atcc 51760t of 96.0 %, followed by photoba ... | 2016 | 27154455 |
decolorization of azo dye c.i. reactive black 5 by ozonation in aqueous solution: influencing factors, degradation products, reaction pathway and toxicity assessment. | in this study, ozonation treatment of c.i. reactive black 5 (rb5) was investigated at various operating parameters. the results showed that the aqueous solution initially containing 200 mg/l rb5 was quickly decolorized at ph 8.0 with an ozone dose of 3.2 g/h. reaction intermediates with m/z 281, 546, 201, 350, 286 and 222 were elucidated using liquid chromatography-mass spectrometry, while sulfate ion, nitrate ion and three carboxylic acids (i.e., oxalic acid, formic acid, and acetic acid) were ... | 2016 | 27054721 |
genomic and physiological analysis reveals versatile metabolic capacity of deep-sea photobacterium phosphoreum ant-2200. | bacteria of the genus photobacterium thrive worldwide in oceans and show substantial eco-physiological diversity including free-living, symbiotic and piezophilic life styles. genomic characteristics underlying this variability across species are poorly understood. here we carried out genomic and physiological analysis of photobacterium phosphoreum strain ant-2200, the first deep-sea luminous bacterium of which the genome has been sequenced. using optical mapping we updated the genomic data and r ... | 2016 | 27039108 |
transformation of benzophenone-type uv filters by chlorine: kinetics, products identification and toxicity assessments. | the present study focused on the kinetics, transformation pathways and toxicity of several benzophenone-type ultraviolet filters (bps) during the water chlorination disinfection process. the transformation kinetics of the studied three bps was found to be second-order reaction, which was dependent on the concentration of bps and chlorine. the second-order rate constants increased from 86.7 to 975 m(-1) s(-1) for oxybenzone, 49.6-261.7 m(-1) s(-1) for 4-hydroxybenzophenone and 51.7-540 m(-1) s(-1 ... | 2016 | 27035274 |
the investigation of different pollutants and operation processes on sludge toxicity in sequencing batch bioreactors. | the influence of different target pollutants and operation modes in sequencing batch bioreactors (sbrs) on sludge toxicity was compared in this study. sludge toxicity was characterized by the inhibiting luminosity through using luminescent bacterium photobacterium phosphoreum (p. phosphoreum) during either gradual acclimation or impaction processes with synthetic wastewater containing high-strength bisphenol a (bpa) or n, n-dimethylformamide (dmf). when the activated sludge was first acclimated ... | 2016 | 26914341 |
biosensitive element in the form of immobilized luminescent photobacteria for detecting ecotoxicants in aqueous flow-through systems. | we demonstrated the possibility of long-term and efficient application of a biosensitive element (be) in the form of photobacterium phosphoreum photobacteria immobilized in poly(vinyl alcohol) (pva) cryogel for detecting various ecotoxicants (zn(2) (+) , cu(2) (+) , hg(2) (+) , pb(2) (+) , 2,4-dichlorophenoxyacetic acid, 2,6-dimethylphenol, pentachlorophenol, coumaphos, malathion, chlorpyrifos and methyl parathion) in flow-through media. the range of detectable concentrations of ecotoxicants was ... | 2016 | 26864478 |
ozonation of the uv filter benzophenone-4 in aquatic environments: intermediates and pathways. | the occurrence of benzophenone-4 (bp-4) in water environments may pose a serious public health hazard due to its potential endocrine disrupting effects. in this work, the intermediates, probable degradation pathways and toxicity changes during ozonation of bp-4 in aqueous solution were systematically investigated. results revealed that alkaline conditions favored the oxidation of bp-4. however, inorganic anions (cl(-), no3(-), so4(2-)), cations (k(+), ca(2+), mg(2+)) and humic acid had no remark ... | 2016 | 26855209 |
where does the toxicity of metal oxide nanoparticles come from: the nanoparticles, the ions, or a combination of both? | the toxicity of metal oxide nanoparticles (nps) has aroused great concern over the past few years. however, there still remains the question whether the toxicity of the metal oxide nps originates from the released ions or the nps themselves. in this study, the metal ion release of cuo, fe2o3, zno, co3o4, cr2o3, and nio nps in aqueous media was investigated, and their contributions to the metal oxide nps' inhibition on the bioluminescence of photobacterium phosphoreum were studied. it was found t ... | 2016 | 26852208 |
volatile organic compounds and photobacterium phosphoreum associated with spoilage of modified-atmosphere-packaged raw pork. | accumulation of volatile organic compounds was monitored in association with sensory quality, bacterial concentrations and culture-independent microbial community analyses in raw pork loin and pork collar during storage under high-oxygen modified atmosphere at +4 °c. of the 48 volatile compounds detected in the pork samples, the levels of acetoin, diacetyl and 3-methyl-1-butanol had the highest correlations with the sensory scores and bacterial concentrations. these compounds accumulated in all ... | 2016 | 26623935 |
enhanced aox accumulation and aquatic toxicity during 2,4,6-trichlorophenol degradation in a co(ii)/peroxymonosulfate/cl⁻ system. | chloride ion is known to affect on degradation kinetics in different ways during ho· and so4(·-)-based advanced oxidation processes (aops). however, its effect on absorbable organic halogen (aox) evolution and acute toxicity of treated water remains unknown, despite the importance of the two parameters in evaluating the applicability of aops. in the present study, co/peroxymonosulfate (co/pms) and uv/hydrogen peroxide (uv/h2o2) treatment of 2,4,6-trichlorophenol was compared in terms of aox form ... | 2016 | 26613359 |
impact of nisin-activated packaging on microbiota of beef burgers during storage. | beef burgers were stored at 4°c in a vacuum in nisin-activated antimicrobial packaging. microbial ecology analyses were performed on samples collected between days 0 and 21 of storage to discover the population diversity. two batches were analyzed using rna-based denaturing gradient gel electrophoresis (dgge) and pyrosequencing. the active packaging retarded the growth of the total viable bacteria and lactic acid bacteria. culture-independent analysis by pyrosequencing of rna extracted directly ... | 2015 | 26546424 |
genetic diversity analysis of isolates belonging to the photobacterium phosphoreum species group collected from salmon products using aflp fingerprinting. | an accurate amplified fragment length polymorphism (aflp) method, including three primer sets for the selective amplification step, was developed to display the phylogenetic position of photobacterium isolates collected from salmon products. this method was efficient for discriminating the three species photobacterium phosphoreum, photobacterium iliopiscarium and photobacterium kishitanii, until now indistinctly gathered in the p. phosphoreum species group known to be strongly responsible for se ... | 2016 | 26513249 |
ecotoxicity monitoring and bioindicator screening of oil-contaminated soil during bioremediation. | a series of toxicity bioassays was conducted to monitor the ecotoxicity of soils in the different phases of bioremediation. artificially oil-contaminated soil was inoculated with a petroleum hydrocarbon-degrading bacterial consortium containing burkholderia cepacia gs3c, sphingomonas gy2b and pandoraea pnomenusa gp3b strains adapted to crude oil. soil ecotoxicity in different phases of bioremediation was examined by monitoring total petroleum hydrocarbons, soil enzyme activities, phytotoxicity ( ... | 2016 | 26491984 |
rapid removal of tetrabromobisphenol a by ozonation in water: oxidation products, reaction pathways and toxicity assessment. | tetrabromobisphenol a (tbbpa) is one of the most widely used brominated flame retardants and has attracted more and more attention. in this work, the parent tbbpa with an initial concentration of 100 mg/l was completely removed after 6 min of ozonation at ph 8.0, and alkaline conditions favored a more rapid removal than acidic and neutral conditions. the presence of typical anions and humic acid did not significantly affect the degradation of tbbpa. the quenching test using isopropanol indicated ... | 2015 | 26430733 |
application of a new integrated sediment quality assessment method to huelva estuary and its littoral of influence (southwestern spain). | a new integrated sediment quality assessment method composed of several assays (particle size profile, total metal content, protease k extraction, total organic carbon, toxicity bioassay with photobacterium phosphoreum and macrobenthic community alteration) that provides a single result, the environmental degradation index (edi), has been developed. the new method was tested on the huelva estuary (southwest of spain), a highly polluted area where metals dissolved in the water of the tinto and od ... | 2015 | 26260104 |
a zeta potential value determines the aggregate's size of penta-substituted [60]fullerene derivatives in aqueous suspension whereas positive charge is required for toxicity against bacterial cells. | the cause-effect relationships between physicochemical properties of amphiphilic [60]fullerene derivatives and their toxicity against bacterial cells have not yet been clarified. in this study, we report how the differences in the chemical structure of organic addends in 10 originally synthesized penta-substituted [60]fullerene derivatives modulate their zeta potential and aggregate's size in salt-free and salt-added aqueous suspensions as well as how these physicochemical characteristics affect ... | 2015 | 26253116 |
selection of bioprotective cultures for preventing cold-smoked salmon spoilage. | biopreservation is a natural technology of food preservation, which consists of inoculating food with microorganisms selected for their antibacterial properties. the objective of this study was to select lactic acid bacteria (lab) to improve the quality of cold-smoked salmon (css). in this work, different strains representative of the 4 dominant species, identified in a previous study by pyrosequencing the 16s rrna gene, were isolated and their spoiling potential in css blocks, sterilized by ion ... | 2015 | 26044337 |
joint toxicity of lead, chromium, cobalt and nickel to photobacterium phosphoreum at no observed effect concentration. | joint toxicity of pb2+, cr3+, co2+ and ni2+ toward photobacterium phosphoreum (ph. phosphoreum) at the no observed effect concentration (noec) was determined through a factorial experiment. a neural network model was designed according to experimental results and employed for toxicity prediction of unary, binary, ternary and quaternary combinations. the mechanism and trends of joint toxicity were interpreted by quantitative structure-activity relationship, michaelis-menten kinetic model, and con ... | 2015 | 26032300 |
[analysis of the dynamics of bioluminescence intensity of luminous bacteria photobacterium phosphoreum]. | this contribution presents the results of analysis of the dynamics of the bioluminescence of luminous bacteria photobacterium phosphoreum imv b-7071 under optimal conditions of their growth. a quasi-harmonic nature of the bacterial bioluminescence dynamics was detected. the observed periods of these changes have similar values compared with those in the earlier defined periods of changes in physicochemical properties of water. the relationship between biorhythms and a quasi-harmonic nature of ch ... | 2015 | 26016027 |
acute toxicity of benzophenone-type uv filters for photobacterium phosphoreum and daphnia magna: qsar analysis, interspecies relationship and integrated assessment. | the hazardous potential of benzophenone (bp)-type uv filters is becoming an issue of great concern due to the wide application of these compounds in many personal care products. in the present study, the toxicities of bps to photobacterium phosphoreum and daphnia magna were determined. next, density functional theory (dft) and comparative molecular field analysis (comfa) descriptors were used to obtain more detailed insight into the structure - activity relationships and to preliminarily discuss ... | 2015 | 25950412 |
[atp pool and bioluminescence in psychrophilic bacteria photobacterium phosphoreum]. | bioluminescence activity and atp pool were investigated in the culture of psychrophilic bacteria photobacterium phosphoreum collected-from the exponential and stationary growth phases, as well as immobilized in polyvinyl alcohol (pva) cryogel. in liquid culture, atp pool remained at an almost a constant level throughout the luminescence cycle (over 100 h). the atp pool in the stationary-phase and pva-immobilizedl cells remained constant throughout their incubation in the medium (over 200 h) and ... | 2015 | 25844450 |
microbiological evolution of gilthead sea bream (sparus aurata) in canary islands during ice storage. | this study analyses the microbiological changes with traditional methods for total mesophilic aerobic, psychrotrophic, aeromonas sp., pseudomonas sp., shewanella putrefaciens, enterobacteriaceae, sulfide-reducing clostridium and photobacterium phosphoreum in muscle, skin and gills of whole ungutted gilthead sea bream (sparus aurata) stored in ice during 18 days. the muscle tissue showed the minor grade of contamination, followed by the skin and the gills, with statistic significance (p < 0.001). ... | 2013 | 25745228 |
[biosensors based on the luminous bacteria photobaterium phosphoreum immobilized in polyvinyl alcohol cryogel for the monitoring of ecotoxicants]. | immobilization of photobacterium phosphoreum bacteria in polyvinyl alcohol cryogel was performed in order to develop biosensors used for ecotoxicant biomonitoring. the immobilization procedure, storage, and application of the immobilized cells for biomonitoring were optimized. it was shown that the immobilized cells demonstrate significantly higher stability and a longer duration of light emission than free bacteria. a discrete analysis of heavy metals and chlorophenols was conducted using the o ... | 2015 | 25707106 |
reduction in toxicity of coking wastewater to aquatic organisms by vertical tubular biological reactor. | we conducted a battery of toxicity tests using photo bacterium, algae, crustacean and fish to evaluate acute toxicity profile of coking wastewater, and to evaluate the performance of a novel wastewater treatment process, vertical tubular biological reactor (vtbr), in the removal of toxicity and certain chemical pollutants. a laboratory scale vtbr system was set up to treat industrial coking wastewater, and investigated both chemicals removal efficiency and acute bio-toxicity to aquatic organisms ... | 2015 | 25706086 |
effect of fenton oxidation on biodegradability, biotoxicity and dissolved organic matter distribution of concentrated landfill leachate derived from a membrane process. | the treatment of concentrated landfill leachate from membrane process is a troublesome issue due to high concentrations of biorecalcitrant pollutants. in this study, the effect of fenton process on dissolved organic matter (dom) distribution (i.e. humic acid (ha), fulvic acid (fa) and hydrophilic fraction (hyi)), chemical forms of toxic organic compounds and metals, and their biotoxicity were investigated. in the concentrated leachate, toluene, ethylbenzene and chlorobenzene predominated in the ... | 2015 | 25660905 |
predictive model for the growth of spoilage bacteria on modified atmosphere packaged atlantic salmon produced in australia. | most existing models for the spoilage of modified atmosphere packed atlantic salmon are based on the growth of the spoilage organism photobacterium phosphoreum. however, there is evidence that this organism is not the specific spoilage organism on salmon produced and packaged in australia. we developed a predictive model for the growth of bacteria in australian-produced atlantic salmon stored under modified atmosphere conditions (30-98% carbon dioxide in nitrogen) at refrigeration temperatures ( ... | 2015 | 25583344 |
oxidation of tetracycline antibiotics induced by fe(iii) ions without light irradiation. | the presence of fe(iii) ions was found to induce degradation of three tetracycline antibiotics (tcs), tetracycline (ttc), oxytetracycline (otc) and chlorotetracycline (ctc), in aqueous solutions without light. the presence of fe(iii) promoted the degradation of tcs in most experimental ph (5.0, 7.0 and 9.0) except at ph 9.0 for ctc. degradation rate constants of ttc, otc and ctc reached maximum ((6.2±0.5)×10(-3) h(-1), (10.6±0.1)×10(-3) h(-1) and (15.9±0.5)×10(-3) h(-1) at ph 7.0, 20 °c) when fe ... | 2015 | 25460769 |
toxicological investigation of soils with the solid-phase flash assay: comparison with other ecotoxicological tests. | a new direct-contact toxicity test, the solid-phase flash assay, which utilises photobacteria in direct contact with soil particles during the exposure, was evaluated on four soil samples. samples htnt1 and htnt2 originated from former military sites in germany, and were highly contaminated with nitroaromatics (approximately 20g/kg), lead and polycyclic aromatic hydrocarbons. samples lmkw1 and lmkw2, from bioremediation stacks in germany, were mainly contaminated with mineral oils. the solid-pha ... | 2014 | 25419927 |
degradation and dechlorination of pentachlorophenol by microwave-activated persulfate. | the degradation performance of pentachlorophenol (pcp) by the microwave-activated persulfate (mw/ps) process was investigated in this study. the results indicated that degradation efficiency of pcp in the mw/ps process followed pseudo-first-order kinetics, and compared with conventional heating, microwave heating has a special effect of increasing the reaction rate and reducing the process time. a higher persulfate concentration and reaction temperature accelerated the pcp degradation rate. mean ... | 2015 | 25328098 |
biomarker discovery and biotherapeutics applications of photosynthetic light-harvesting and bioluminescence light-emitting chromophore-protein complexes in stem cell biology and regenerative medicine. | we have since the 1970's embarked on the development of biologically derived fluorophore-protein complexes that will find applications in the communicable and non-communicable disease etiology processes and their cures. we have since then become largely successful in these endeavors along with interspersed contributions also from investigators who have generally restricted to working in confined disciplines. their encompassment with our works as this investigator has traversed his definitely cho ... | 2014 | 25157447 |
evaluation on joint toxicity of chlorinated anilines and cadmium to photobacterium phosphoreum and qsar analysis. | the individual ic50 (the concentrations causing a 50% inhibition of bioluminescence after 15min exposure) of cadmium ion (cd) and nine chlorinated anilines to photobacterium phosphoreum (p. phosphoreum) were determined. in order to evaluate the combined effects of the nine chlorinated anilines and cd, the toxicities of chlorinated anilines combined with different concentrations of cd were determined, respectively. the results showed that the number of chlorinated anilines manifesting synergy wit ... | 2014 | 25058936 |
population dynamics of vibrio and pseudomonas species isolated from farmed tasmanian atlantic salmon (salmo salar l.): a seasonal study. | vibrio and pseudomonas species have been shown to be part of the normal microbiota of atlantic salmon (salmo salar l.), with some strains causing disease in fish. the factors affecting their prevalence and persistence in the salmon gut, however, have not been well studied. in this study, we collected 340 vibrio and 150 pseudomonas isolates from the hindgut of farmed tasmanian atlantic salmon, fed with two commercially available diets. samples were collected every 6-8 weeks between july 2011 and ... | 2014 | 25027277 |
[investigation of physiological features of luminous bacteria photobacterium phosphoreum imv b-7071]. | bioluminescence of the strain of luminous bacteria photobacterium phosphoreum imv b-7071 has been studied. the strain was characterized by a high level of luminescence, high specific growth rate, short lag period and generation time. the authors have established the action of ph, temperature, concentration of naci on the level of bioluminescence. the maximum level of bioluminescence was observed in nacl concentration range of 2 to 8%, in the range of ph from 5.5 to 9.1 and a temperature range of ... | 2015 | 25007443 |
photobacterium piscicola sp. nov., isolated from marine fish and spoiled packed cod. | five isolates from marine fish (w3(t), wm, w1s, s2 and s3) and three isolates misclassified as photobacterium phosphoreum, originating from spoiled modified atmosphere packed stored cod (ncimb 13482 and ncimb 13483) and the intestine of skate (ncimb 192), were subjected to a polyphasic taxonomic study. phylogenetic analysis of 16s rrna gene sequences showed that the isolates were members of the genus photobacterium. sequence analysis using the gapa, gyrb, pyrh, reca and rpoa loci showed that the ... | 2014 | 24951451 |
the joint effects on photobacterium phosphoreum of metal oxide nanoparticles and their most likely coexisting chemicals in the environment. | metal oxide nanoparticles (nps) have been used increasingly and are likely to accumulate in natural water bodies, where they encounter and interact with other environmental chemicals. these interactions result in joint effects on biological systems and the environment. however, compared with the intensive research examining the toxicities of individual nps, the toxicities of np mixtures remain relatively unexplored. in this work, we studied the joint effects of nps and their most likely coexisti ... | 2014 | 24911590 |
surfactants present complex joint effects on the toxicities of metal oxide nanoparticles. | the potential toxicities of nanoparticles (nps) have been intensively discussed over the past decade. in addition to their single toxicities, nps can interact with other environmental chemicals and thereby exert joint effects on biological systems and the environment. the present study investigated the combined toxicities of nps and surfactants, which are among the chemicals that most likely coexist with nps. photobacterium phosphoreum was employed as the model organism. the results indicate tha ... | 2014 | 24875914 |
genome sequence of luminous piezophile photobacterium phosphoreum ant-2200. | bacteria of the genus photobacterium thrive worldwide in oceans and show substantially varied lifestyles, including free-living, commensal, pathogenic, symbiotic, and piezophilic. here, we present the genome sequence of a luminous, piezophilic photobacterium phosphoreum strain, ant-2200, isolated from a water column at 2,200 m depth in the mediterranean sea. it is the first genomic sequence of the p. phosphoreum group. an analysis of the sequence provides insight into the adaptation of bacteria ... | 2014 | 24744322 |
effect of water quality on mercury toxicity to photobacterium phosphoreum: model development and its application in natural waters. | mercury (hg) compounds are widely distributed toxic environmental and industrial pollutants and they may bring danger to growth and development of aquatic organisms. the distribution of hg species in the 3 percent nacl solution was calculated using the chemical equilibrium model visual minteq, which demonstrated that hg was mainly complexed by chlorides in the ph range 5.0-9.0 and the proportions of hgcl4(2-), hgcl3(-) and hgcl2(aq) reached to 95 percent of total hg. then the effects of cations ... | 2014 | 24726934 |
balance between herbicidal activity and toxicity effect: a case study of the joint effects of triazine and phenylurea herbicides on selenastrum capricornutum and photobacterium phosphoreum. | the use of herbicide mixtures has become a cost-effective strategy against the evolution of herbicide resistance to protect global food production. much research has focused on investigating either the herbicidal activities or the toxicity effects of herbicides; however, few of them have investigated both factors. this study investigates the balance between herbicidal activity for selenastrum capricornutum and toxicity effect toward photobacterium phosphoreum by determining the joint effects of ... | 2014 | 24681700 |
study on the variation rules of the joint effects for multicomponent mixtures containing cyanogenic toxicants and aldehydes based on the transition state theory. | although the study of the variation rules of the joint effects for multicomponent mixtures has gained increasing attention, it still remains unclear how the variation occurs and what the relationships between the joint effects of multicomponent mixtures and their corresponding binary mixtures are. to explain how the variation occurs, this study first proposes a hypothesis on the variation rules of the joint effects using the well-known transition state theory. the hypothesis concluded that the j ... | 2014 | 24418495 |
teratogenic effects of organic extracts from the pearl river sediments on xenopus laevis embryos. | toxicity of organic extracts from the pearl river sediments was investigated with xenopus laevis embryos. the effects of sediment organic extracts on the mortality, body length and malformation of x. laevis embryos were tested by the frog embryo teratogenesis assay-xenopus (fetax). the 96-h lc₅₀ values for x. laevis embryos ranged from 62 to 137 g/l (g extracted sediment per l), and the toxicity effect on body length of larvae was not significant under 20 g/l. however, the teratogenic effects pr ... | 2014 | 24361698 |
shelf life extension of whole norway lobster nephrops norvegicus using modified atmosphere packaging. | once a nuisance by-catch, today the norway lobster (nephrops norvegicus) is a valuable uk fisheries commodity. unfortunately, the species is very susceptible to quality deterioration post harvest as it quickly develops black spots and also spoils rapidly due to bacterial growth. treatment with chemicals can stop the blackening and carefully monitored cold storage can result in a sensory shelf life of up to 6.5 days. the high susceptibility to spoilage greatly restricts the extent to which n. nor ... | 2013 | 24201017 |
development of a model to predict the effect of water chemistry on the acute toxicity of cadmium to photobacterium phosphoreum. | cadmium (cd) compounds are widely distributed toxic environmental and industrial pollutants, and they may bring danger to growth and development of aquatic organisms. the effects of ca(2+) (as cacl2), mg(2+) (as mgso4), k(+) (as kcl), ph and complexants (edta, the commercial dom, and three homemade doms) on cd toxicity to photobacterium phosphoreum were evaluated in standardized 15 min acute toxicity tests. increases in ca(2+) concentration resulted in higher ec50 values, indicating the competit ... | 2013 | 24041821 |
quantitative structure activity relationships (qsar) for binary mixtures at non-equitoxic ratios based on toxic ratios-effects curves. | the present study proposed a qsar model to predict joint effects at non-equitoxic ratios for binary mixtures containing reactive toxicants, cyanogenic compounds and aldehydes. toxicity of single and binary mixtures was measured by quantifying the decrease in light emission from the photobacterium phosphoreum for 15 min. the joint effects of binary mixtures (tu sum) can thus be obtained. the results showed that the relationships between toxic ratios of the individual chemicals and their joint eff ... | 2013 | 23930105 |
effects of hydrostatic pressure on growth and luminescence of a moderately-piezophilic luminous bacteria photobacterium phosphoreum ant-2200. | bacterial bioluminescence is commonly found in the deep sea and depends on environmental conditions. photobacterium phosphoreum ant-2200 has been isolated from the nw mediterranean sea at 2200-m depth (in situ temperature of 13°c) close to the antares neutrino telescope. the effects of hydrostatic pressure on its growth and luminescence have been investigated under controlled laboratory conditions, using a specifically developed high-pressure bioluminescence system. the growth rate and the maxim ... | 2013 | 23818946 |
the underlying toxicological mechanism of chemical mixtures: a case study on mixture toxicity of cyanogenic toxicants and aldehydes to photobacterium phosphoreum. | intracellular chemical reaction of chemical mixtures is one of the main reasons that cause synergistic or antagonistic effects. however, it still remains unclear what the influencing factors on the intracellular chemical reaction are, and how they influence on the toxicological mechanism of chemical mixtures. to reveal this underlying toxicological mechanism of chemical mixtures, a case study on mixture toxicity of cyanogenic toxicants and aldehydes to photobacterium phosphoreum was employed, an ... | 2013 | 23811331 |
toxicity testing with luminescent bacteria--characterization of an automated method for the combined assessment of acute and chronic effects. | the luminescent bacteria test according to en iso 11348 is frequently applied in (eco) toxicity testing and is applicable for a huge variety of environmental and industrial samples. a big disadvantage of this method is the very short exposure time, which is expressed in a low sensitivity in regard to substances with a delayed effect. chronic effects, i.e. interference with cell growth, cannot be assessed with this conventional standard method. the goal of this research was to develop an automate ... | 2013 | 23806483 |
the toxicity of cadmium to three aquatic organisms (photobacterium phosphoreum, daphnia magna and carassius auratus) under different ph levels. | this study investigated the effect of ph on cadmium toxicity to three aquatic organisms: photobacterium phosphoreum, daphnia magna and carassius auratus. the acute toxicity of cd(2+) to p. phosphoreum and d. magna at five ph values (5.0, 6.0, 7.0, 8.0, and 9.0) was assessed by calculating ec50 values. we determined that cd(2+) was least toxic under acidic conditions, and d. magna was more sensitive to the toxicity of cd than p. phosphoreum. to evaluate cd(2+)-induced hepatic oxidative stress in ... | 2013 | 23769002 |
detection of heavy metal resistance bioluminescence bacteria using microplate bioassay method. | effects of different heavy metals on vibrio harveyi, v. fischeri, photobacterium phosphoreum and p. leiognathi were examined. checkerboard assay was used for the detection of the natural metal tolerance levels of a large number of marine luminous eubacteria. 57 strains of luminous bacteria were investigated for their natural patterns of heavy metal tolerance. the behaviors of these strains were not homogeneous with respect to all metals tested, even within the strains belonging to the same genus ... | 2012 | 23741856 |
ozonation of trimethoprim in aqueous solution: identification of reaction products and their toxicity. | this work aimed to better understand the ozonation process of a typical antibiotic pharmaceutical, trimethoprim in aqueous solution. the parent compound was almost completely degraded with ozone dose up to 3.5 mg/l with no mineralization. twenty one degradation products were identified using an electrospray quadrupole time-of-flight mass spectrometer. several ozonation pathways were proposed including hydroxylation, demethylation, carbonylation, deamination and methylene group cleavage. two spec ... | 2013 | 23541309 |
toxicity-based assessment of the treatment performance of wastewater treatment and reclamation processes. | the reclamation and reuse of wastewater is one of the possible ways to relieve the serious fresh water resource crisis in china. efficient reclamation treatment technologies ensure the safe reuse of reclaimed water. in order to screen out and evaluate technologies appropriate for reclamation treatment, a great deal of efforts have been brought to bear. in the present study, a toxicity-based method including a photobacterium phosphoreum test for acute toxicity and sos/umu test for genotoxicity, a ... | 2012 | 23505863 |
effect of tritium on luminous marine bacteria and enzyme reactions. | the paper studies chronic effect of tritiated water, hto, (0.0002-200 mbq/l) on bioluminescent assay systems: marine bacteria photobacterium phosphoreum (intact and lyophilized) and coupled enzyme reactions. bioluminescence intensity serves as a marker of physiological activity. linear dependencies of bioluminescent intensity on exposure time or radioactivity were not revealed. three successive stages in bacterial bioluminescence response to hto were found: (1) absence of the effect, (2) activat ... | 2013 | 23410594 |
development of a rapid real-time pcr method as a tool to quantify viable photobacterium phosphoreum bacteria in salmon (salmo salar) steaks. | a specific real-time pcr quantification method combined with a propidium monoazide sample treatment step was developed to determine quantitatively the viable population of the photobacterium phosphoreum species group in raw modified-atmosphere-packed salmon. primers were designed to amplify a 350-bp fragment of the gyrase subunit b gene (gyrb) of p. phosphoreum. the specificity of the two primers was demonstrated by using purified dna from 81 strains of 52 different bacterial species. when these ... | 2013 | 23396343 |
[acute toxicity of antibiotics and anaerobic digestion intermediates in pharmaceutical wastewaters]. | in order to determine the toxicity of antibiotics and anaerobic digestion intermediates on anaerobic treatment of pharmaceutical wastewaters containing antibiotics, the single and joint toxicities of some antibiotics and intermediates to photobacterium phosphoreum were tested by using the 15-min half inhibitory concentration (15 min-ic50) at ph = 7.00 +/- 0.05. the results showed that the 15 min-ic50 of ethanol, acetate, propionate and butyrate were 19.40, 20.71, 10.47 and 12.17 g x l(-1), respe ... | 2012 | 23379166 |
evaluation of the spoilage potential of bacteria isolated from spoiled raw salmon (salmo salar) fillets stored under modified atmosphere packaging. | the spoilage potential of eight bacterial groups/species (serratia spp., hafnia alvei, brochothrix thermosphacta, carnobacterium maltaromaticum, shewanella baltica, lactococcus piscium, photobacterium phosphoreum, "other enterobacteriaceae" [containing one strain of moellerella sp., morganella sp. and pectobacterium sp.]) isolated from spoiled raw salmon fillets stored under modified atmosphere packaging (map) was evaluated by inoculation into sterile raw salmon cubes followed by storage for 12d ... | 2012 | 23290229 |
sensitivity to pulsed light technology of several spoilage and pathogenic bacteria isolated from fish products. | the effect of pulsed light (pl) on the inactivation of six fish spoilage bacteria (photobacterium phosphoreum, serratia liquefaciens, shewanella putrefaciens, brochothrix thermosphacta, pseudomonas group i, and pseudomonas groups iii and iv), six listeria monocytogenes isolates from fish products, and one strain of listeria innocua was evaluated. for all tested strains, grown at 4°c (temperature to process, store, and distribute convenient, lightly preserved fish products), the maximum detectabl ... | 2012 | 23127714 |
bioluminescence as a tool for studying detoxification processes in metal salt solutions involving humic substances. | the paper considers effects of humic substances (hs), as natural attenuators of toxicity, on solutions of model inorganic pollutants, metal salts - pb(no(3))(2), сосl(2), cuso(4), eu(no(3))(3), сrсl(3), and k(3)[fe(сn)(6)]. luminous bacteria photobacterium phosphoreum and bioluminescent system of coupled enzymatic reactions were used as bioassays to monitor toxicity of salt solutions. the ability of hs to decrease or increase toxicity was demonstrated. detoxifying concentrations of hs were deter ... | 2012 | 23123596 |
evaluation of joint toxicity of nitroaromatic compounds and copper to photobacterium phosphoreum and qsar analysis. | the individual toxicities of cu and 11 nitroaromatic compounds to photobacterium phosphoreum were determined. the toxicity was expressed as the concentrations causing a 50% inhibition of bioluminescence after 15 min exposure (ic(50)). to evaluate the joint effect between the metal ion and the 11 nitroaromatic compounds, the joint toxicity of cu and 11 nitroaromatic compounds were measured at different cu concentrations (0.2ic(50), 0.5ic(50) and 0.8ic(50)), respectively. the result shows that the ... | 2012 | 23089062 |
exploring qstr analysis of the toxicity of phenols and thiophenols using machine learning methods. | there is an increasing need for the rapid safety assessment of chemicals by both industries and regulatory agencies throughout the world. in silico techniques are practical alternatives in the environmental hazard assessment. in this background, quantitative structure-toxicity relationship (qstr) analysis has been performed on toxicity of phenols and thiophenols to photobacterium phosphoreum. the techniques of classification and regression trees (cart) and least squares support vector regression ... | 2012 | 23068157 |
loss-of-function mutation in bi-functional marine bacterial sialyltransferase. | an α2,3-sialyltransferase produced by photobacterium phosphoreum jt-ish-467 is a bi-functional enzyme showing both α2,3-sialyltransferase and α2,3-linkage specific sialidase activity. to date, the crystal structures of several sialyltransferases have been solved, but the roles of amino acid residues around the catalytic site have not been completely clarified. hence we performed a mutational study using α2,3-sialyltransferase cloned from p. phosphoreum jt-ish-467 as a model enzyme to study the r ... | 2012 | 22972324 |
effects of americium-241 and humic substances on photobacterium phosphoreum: bioluminescence and diffuse reflectance ftir spectroscopic studies. | the integral bioluminescence (bl) intensity of live photobacterium phosphoreum cells (strain 1883 ibso), sampled at the stationary growth stage (20 h), was monitored for further 300 h in the absence (control) and presence of (241)am (an α-emitting radionuclide of a high specific activity) in the growth medium. the activity concentration of (241)am was 2 kbq l(-1); [(241)am]=6.5×10(-11) m. parallel experiments were also performed with water-soluble humic substances (hs, 2.5 mg l(-1); containing o ... | 2013 | 22795580 |
model of hormesis and its toxicity mechanism based on quorum sensing: a case study on the toxicity of sulfonamides to photobacterium phosphoreum. | during the past two decades, the phenomenon of hormesis has gained increasing recognition in environmental and toxicological communities. however, the mechanistic understanding of hormesis, to date, is extremely limited. herein is proposed a novel parametric model with a mechanistic basis and two model-based parameters for hormesis that was successfully applied to the hormetic dose-response observed in the chronic toxicity of sulfonamides on photobacterium phosphoreum. on the basis of the method ... | 2012 | 22715968 |
photolysis of chlortetracycline in aqueous solution: kinetics, toxicity and products. | the aqueous photodegradation of the widely used antibiotic chlortetracycline (ctc) was investigated under simulated sunlight. the quantum yield of photodegradation increased from 3.3 x 10(-4) to 8.5 x 10(-3) within the ph range of 6.0 to 9.0. the presence of ca2+, fe3+, and no(-3) enhanced the photodegradation of ctc, whereas mg2+, mn2+, and zn2+ inhibited the degradation with the order mn2+ > zn2+ > mg2+ at ph 7.3. the monovalent cations (na+ and k+) had negligible effect on the photolysis of c ... | 2012 | 22655385 |
quantitative structure-activity relationship for prediction of the toxicity of phenols on photobacterium phosphoreum. | quantitative structure-activity relationships (qsar) is an alternative to experimental toxicity testing and recommended by environmental protection agencies. in this background, an accurate and reliable qsar model of 18 phenols for their toxicity to photobacterium phosphoreum was developed using mechanistically interpretable molecular structural descriptors. the qsar model was developed by stepwise multiple linear regression and the reliability of the model was evaluated by internal and external ... | 2012 | 22562268 |
influence factors of multicomponent mixtures containing reactive chemicals and their joint effects. | organic chemicals usually coexist as a mixture in the environment, and the mixture toxicity of organic chemicals has received increased attention. however, research regarding the joint effects of reactive chemicals is lacking. in this study, we examined two kinds of reactive chemicals, cyanogenic toxicants and aldehydes and determined their joint effects on photobacterium phosphoreum. three factors were found to influence the joint effects of multicomponent mixtures containing reactive chemicals ... | 2012 | 22503635 |
characterisation of the spoilage microbiota in raw salmon (salmo salar) steaks stored under vacuum or modified atmosphere packaging combining conventional methods and pcr-ttge. | in order to characterise the spoilage related to microbiota of raw salmon, a combination of culture-dependent and -independent methods, including pcr-ttge, was used to analyse 3 raw salmon batches stored for 3 days at chilled temperature in modified atmosphere packaging (map) (50% co₂/50% n₂) or under vacuum. sensory evaluation, microbiological enumeration and chemical analysis were performed after 3, 7 and 10 days of storage. at the onset of spoilage, 65 bacterial isolates were picked from the ... | 2011 | 22265297 |
the effect of crowding stress on bacterial growth and sensory properties of chilled atlantic salmon fillets. | atlantic salmon were subjected to minimal preslaughter crowding stress (control), short-term crowding for 20 min (ss-group), or long-term crowding for 24 h (ls-group). the fish were filleted prerigor, cut into 270 g pieces, and packaged in modified atmosphere (60% co₂ and 40% n₂). fillet quality analyses were determined during 22 d of storage at 0.3 °c. bacterial growth and unpleasant sensory properties increased earlier in the ls-group. the negative effects of long-term preslaughter stress were ... | 2012 | 22260135 |
solubility properties in polymers and biological media. 7. an analysis of toxicant properties that influence inhibition of bioluminescence in photobacterium phosphoreum (the microtox test). | 1986 | 22185313 | |
acute toxicity and n-octanol/water partition coefficients of substituted thiophenols: determination and qsar analysis. | the acute toxicity (-logec(50)) to photobacterium phosphoreum and the n-octanol/water partition coefficient (logk(ow)) of 31 kinds of substituted thiophenols were determined at 298.15k. the -logec(50) values of studied chemicals are between 4.26 and 5.89. their logk(ow) values are between 1.34 and 4.02. comparative molecular field (comfa) and comparative molecular similarity index analysis (comsia) models established were successful in predicting -logec(50) and logk(ow) values of halogenated, me ... | 2011 | 22154146 |
Acute Toxicity of Alkylpolyglucosides to Vibrio fischeri, Daphnia magna and Microalgae: A Comparative Study. | In this paper, toxicity values of alkylpolyglucosides have been determined by applying the 24-h immobilization test with Daphnia magna, the LumiStox(®) 300 test which employs the luminescent bacteria Photobacterium phosphoreum and the test with Selenastrum capricornutum. Three alkylpolyglucosides with different alkyl chain and degree of polymerisation have been tested. For all tests, the results indicated that Vibrio fischeri was more sensitive to toxic effects from alkylpolyglucosides than was ... | 2011 | 22127434 |
Atomic charges of individual reactive chemicals in binary mixtures determine their joint effects: An example of cyanogenic toxicants and aldehydes. | Environmental contaminants are usually encountered as mixtures, and many of these mixtures yield synergistic or antagonistic effects attributable to an intracellular chemical reaction that pose a potential threat on ecological systems. However, how atomic charges of individual chemicals determine their intracellular chemical reactions, and then determine the joint effects for mixtures containing reactive toxicants, is not well understood. To address this issue, the joint effects between cyanogen ... | 2011 | 22105991 |
Influences of two antibiotic contaminants on the production, release and toxicity of microcystins. | The influences of spiramycin and amoxicillin on the algal growth, production and release of target microcystins (MCs), MC-LR, MC-RR and MC-YR, in Microcystis aeruginosa were investigated through the seven-day exposure test. Spiramycin were more toxic to M. aeruginosa than amoxicillin according to their 50 percent effective concentrations (EC(50)) in algal growth, which were 1.15 and 8.03µg/l, respectively. At environmentally relevant concentrations of 100ng/l-1µg/l, spiramycin reduced the total ... | 2012 | 22074855 |
historical microbiology: revival and phylogenetic analysis of the luminous bacterial cultures of m. w. beijerinck. | luminous bacteria isolated by martinus w. beijerinck were sealed in glass ampoules in 1924 and 1925 and stored under the names photobacterium phosphoreum and 'photobacterium splendidum'. to determine if the stored cultures were viable and to assess their evolutionary relationship with currently recognized bacteria, portions of the ampoule contents were inoculated into culture medium. growth and luminescence were evident after 13 days of incubation, indicating the presence of viable cells after m ... | 2011 | 22066815 |
The joint effects of sulfonamides and their potentiator on Photobacterium phosphoreum: differences between the acute and chronic mixture toxicity mechanisms. | Organisms are typically exposed to mixtures of chemicals over long periods of time; thus, chronic mixture toxicity analysis is the best way to perform risk assessment in regards to organisms. However, most studies focus on the acute mixture toxicity. To investigate the difference between chronic mixture toxicity and acute mixture toxicity, Photobacterium phosphoreum were exposed to chronic (24 h exposure) and acute (15 min exposure) toxicity of single sulfonamide (SA) and their potentiator (trim ... | 2012 | 21944043 |
Hydrophobicity-dependent QSARs to predict the toxicity of perfluorinated carboxylic acids and their mixtures. | Perfluorinated carboxylic acids (PFCAs) have wide industrial applications because of their unique physicochemical characteristics. However, data on the toxicity of much of this chemical class is lacking, particularly with regard to mixture toxicity. In this study, the toxicity of individual PFCAs and their mixtures to Photobacterium phosphoreum were observed. There was a tendency of increasing toxicity from C3 to C14 PFCA and a tendency of decreasing toxicity from C14 to C18 PFCA because of "the ... | 2011 | 21843807 |
the effect of temperature on the bacterial load and microbial composition in norway lobster (nephrops norvegicus) tail meat during storage. | aims:ôçé the aim of this study was to update and extend our knowledge of the bacterial load and microbial composition in norway lobster (nephrops norvegicus) under commercially relevant storage conditions to optimize handling procedures. methods and results:ôçé total viable counts were performed at different storage temperatures (0, 4, 8, 10, 12 or 16-¦c) and after different storage times (1-7ôçâdays). storage at 16-¦c was found to be most detrimental, and storage at 0-¦c was found to be optimal ... | 2011 | 21689222 |
seafood quality analysis: molecular identification of dominant microbiota after ice storage on several general growth media. | this study points out the limitations of several general growth media frequently used in seafood research by a systematic identification of the microorganisms on fish samples during ice storage unable to grow on those media. aerobic psychrotrophic count (apc), replication on various general media and total cultivable microbial community denaturing gradient gel electrophoresis (dgge) analysis revealed that many potential spoilage microorganisms were overlooked. those microorganisms overlooked by ... | 2011 | 21645815 |
effect of americium-241 on luminous bacteria. role of peroxides. | the effect of americium-241 ((241)am), an alpha-emitting radionuclide of high specific activity, on luminous bacteria photobacterium phosphoreum was studied. traces of (241)am in nutrient media (0.16-6.67 kbq/l) suppressed the growth of bacteria, but enhanced luminescence intensity and quantum yield at room temperature. lower temperature (4 -¦c) increased the time of bacterial luminescence and revealed a stage of bioluminescence inhibition after 150 h of bioluminescence registration start. the r ... | 2011 | 21388726 |
optimal conditions for stability of photoemission and freeze drying of two luminescent bacteria for use in a biosensor. | bioluminescent bacteria have been used for many years for biotoxicological analysis. one of the main concerns with this microorganism is the low experimental repeatability when subjected to external factors. the aim of the present study was to obtain accurate, sensitive, and repeatable measurements with stable signals (during the detection and over days) for application in a water-analysis device for the detection of pollutants. growth conditions were tested and optimized. an optimal freeze-dryi ... | 2011 | 21191881 |
acute toxicity evaluation of explosive wastewater by bacterial bioluminescence assays using a freshwater luminescent bacterium, vibrio qinghaiensis sp. nov. | the compositions of explosive wastewater generated from tnt (2,4,6-trinitrotoluene) purification stage were characterized by using uv-vis spectroscopy, fourier transform infrared spectroscopy (ftir), high performance liquid chromatograph (hplc) and gas chromatograph/mass spectroscopy (gc/ms). the acute toxicity was evaluated by bacterium bioluminescence assay using a freshwater luminescent bacterium (vibrio qinghaiensis sp. nov.) and a marine luminescent bacterium (photobacterium phosphoreum). t ... | 2010 | 21185645 |
detoxification of am-241 solutions by humic substances: bioluminescent monitoring. | the study addresses the effect of humic substances on marine luminous bacteria photobacterium phosphoreum exposed to am-241 (3,000 bq l(-1), water solution). luminescent intensity of the bacteria was applied as a marker of their physiological activity. humic substances have been found to reduce the effect of am-241 on luminescence, decrease damage to cells, and change distribution of am-241 between bacterial cells and intercellular media. it was shown that water-soluble humic substances, being p ... | 2010 | 21174201 |
toxicity prediction of antibiotics on luminescent bacteria, photobacterium phosphoreum, based on their quantitative structure-activity relationship models. | acute toxicity (ec₅₀₋₃₀ min) and chronic toxicity (ec₅₀₋₂₄ h) of 21 antibiotics on photobacterium phosphoreum was observed and quantitative structure-activity relationship (qsar) models were developed, respectively. by comparing these two qsar models, the following model was established, log(1/ec₅₀₋₂₄ h) = 1.8283 + 1.1503log(1/ec ₅₀₋₃₀ min) + 0.2872elomo - 0.0901dm + 0.0003pmiz + 0.0088psa - 0.0382sd, r² = 0.8513, where elomo is lowest occupied molecular energy, dm is dipole moment, pmiz is prin ... | 2010 | 21107523 |
relationships between acute toxicities of para nitrophenol (p-np) and nitrobenzene (nb) to daphnia magna and photobacterium phosphoreum: physicochemical properties and metabolites under anaerobic/aerobic sequentials. | in this study, the acute toxicities of nitrobenzene (nb) and para nitrophenol (p-np) were investigated in a high rate sequential anaerobic migrating blanket (ambr)/aerobic completely stirred tank reactor (cstr) using microtox and daphnia magna tests. after sequential anaerobic and aerobic treatments, the inhibitions in the microtox bacteria decreased from an initial 78.10-48.20% and 4.00%, respectively, in wastewater containing 40.00 mg/l p-np. the inhibitions of the influent wastewater containi ... | 2010 | 21035948 |
enhanced nitrogen removal from pharmaceutical wastewater using sba-anammox process. | efficient biological nitrogen removal from pharmaceutical wastewater has been focused recently. the present study dealt with the treatment of colistin sulfate and kitasamycin manufacturing wastewater through anaerobic ammonium oxidation (anammox). the biotoxicity assay on luminescent bacterium photobacterium phosphoreum (t3 mutation) showed that the pharmaceutical wastewater imparted severe toxicity with a relative luminosity of 3.46% ± 0.45%. during long-term operation, the cumulative toxicity ... | 2011 | 20832098 |
biodegradable gelatin-chitosan films incorporated with essential oils as antimicrobial agents for fish preservation. | essential oils of clove (syzygium aromaticum l.), fennel (foeniculum vulgare miller), cypress (cupressus sempervirens l.), lavender (lavandula angustifolia), thyme (thymus vulgaris l.), herb-of-the-cross (verbena officinalis l.), pine (pinus sylvestris) and rosemary (rosmarinus officinalis) were tested for their antimicrobial activity on 18 genera of bacteria, which included some important food pathogen and spoilage bacteria. clove essential oil showed the highest inhibitory effect, followed by ... | 2010 | 20688230 |
evaluation and structure-activity relationship study of acute toxicity of naphthoquinones to photobacterium phosphoreum, photobacterium t3b. | the acute toxicities of five naphthoquinone compounds to photobacterium phosphoreum were determined. we evaluated the mechanism of toxicity using the structure-activity relationship technique. the results showed that some factors, including the species of substituents, shape/size of molecule and oil-water partition coefficient (log p) played the important roles in the interaction between the naphthoquinones and the target. among of these, the toxicities of atovaquone and buparvaquone were lower ... | 2010 | 20640400 |
improved detection of toxic chemicals by photobacterium phosphoreum using modified boss medium. | a bioluminescent bacterium photobacterium phosphoreum has been used widely as an indicator of pollutants where the presence of toxic chemicals decreases light output. the optimum conditions for the growth and bioluminescence of p. phosphoreum kctc 2852 were investigated using modified boss medium and two environmental conditions (ph and temperature). optimized conditions that supported growth of p. phosphoreum with high bioluminescence intensities were: nacl (20 g/l), glycerol (2.5 g/l), peptone ... | 2010 | 20637650 |
occurrence and role of lactic acid bacteria in seafood products. | lactic acid bacteria (lab) in fish flesh has long been disregarded because the high post-mortem ph, the low percentage of sugars, the high content of low molecular weight nitrogenous molecules and the low temperature of temperate waters favor the rapid growth of ph-sensitive psychrotolerant marine gram-negative bacteria like pseudomonas, shewanella and photobacterium. in seafood packed in both vacuum (vp) and modified atmosphere (map) packaging commonly co(2) enriched, the growth of the gram-neg ... | 2010 | 20630312 |
comparisons of vibrio fischeri, photobacterium phosphoreum, and recombinant luminescent using escherichia coli as bod measurement. | to shorten the time needed to measure biochemical oxygen demand (bod) in water samples and to provide a rapid feedback of the real condition of water quality, we tested and evaluated the validity and reliability of luminescent bacteria vibrio fischeri, photobacterium phosphoreum, and recombinant escherichia coli as potential indicators of bod in the domestic wastewaters. the results indicate that the luminescence intensities of these strains are dependent on temperature, ph, and bod concentratio ... | 2010 | 20390863 |