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characteristics of arg-carrying plasmidome in the cultivable microbial community from wastewater treatment system under high oxytetracycline concentration.studies on antibiotic production wastewater have shown that even a single antibiotic can select for multidrug resistant bacteria in aquatic environments. it is speculated that plasmids are an important mechanism of multidrug resistance (mdr) under high concentrations of antibiotics. herein, two metagenomic libraries were constructed with plasmid dna extracted from cultivable microbial communities in a biological wastewater treatment reactor supplemented with 0 (control) or 25 mg/l of oxytetracyc ...201829332216
purinergic signalling displays an anti-inflammatory profile in the spleen of fish experimentally infected with aeromonas caviae: modulation of the immune response.extracellular adenosine triphosphate (atp) and its metabolite adenosine (ado) are recognized as key mediators of immune and inflammatory responses. depending on its concentration, atp may act as an immunostimulant or immunodepressant, while ado levels display an anti-inflammatory profile. the aim of this study was to evaluate whether splenic purinergic signalling is capable of modulating immune and inflammatory responses in fish experimentally infected with aeromonas caviae. triphosphate diphosp ...201729265378
aeromonas caviae alters the activities of ecto-enzymes that hydrolyze adenine nucleotides in fish thrombocytes.it is recognized that the purinergic system, through the activities of ectonucleoside triphosphate diphosphohydrolase (e-ntpdase), ecto-5'-nucleotidase (e-5'-nucleotidase), and ecto-adenosine deaminase (e-ada), is involved in the regulation and modulation of the physiological and pathological events linked to hemostasis. this occurs due to the role of adenosine diphosphate (adp) in the activation and recruitment of platelets, and the role of adenosine (ado) in the inhibition of platelet activati ...201729253595
speciation, clinical profile & antibiotic resistance in aeromonas species isolated from cholera-like illnesses in a tertiary care hospital in north india.aeromonas species have been reported to cause various illnesses in humans such as wound infections, septicaemia, peritonitis and pneumonia. their role in causation of cholera-like illness is also being increasingly recognized. this retrospective study was done to know the presence of aeromonas as a cause of acute diarrhoea in a tertiary care hospital and to find the common species of aeromonas causing diarrhoea and their antibiotic susceptibility patterns.201729205196
diversity of bacterial lactase genes in intestinal contents of mice with antibiotics-induced diarrhea.to investigate the diversity of bacterial lactase genes in the intestinal contents of mice with antibiotics-induced diarrhea.201729204058
presence of mcr-3 variant in aeromonas caviae, proteus mirabilis, and escherichia coli from one domestic duck.the rapid rise and dissemination of mdr bacteria is a major threat to public health worldwide, and has narrowed the treatment options for infections caused by these bacteria (1).….201729203482
aeromonas caviae inhibits hepatic enzymes of the phosphotransfer network in experimentally infected silver catfish: impairment on bioenergetics.several studies have been demonstrated that phosphotransfer network, through the adenylate kinase (ak) and pyruvate kinase (pk) activities, allows for new perspectives leading to understanding of disease conditions associated with disturbances in energy metabolism, metabolic monitoring and signalling. in this sense, the aim of this study was to evaluate whether experimental infection by aeromonas caviae alters hepatic ak and pk activities of silver catfish rhamdia quelen. hepatic ak and pk activ ...201729193157
bacterial population and chemicals in bioaerosols from indoor environment: sludge dewatering houses in nine municipal wastewater treatment plants.municipal wastewater treatment plants (mwtps) are regarded as sources of airborne microorganisms. sludge dewatering house (sdh) is one of the most serious indoor bioaerosol pollution treatment sectors in mwtps. in this study, properties of bioaerosols from sdhs of nine mwtps were investigated in china. results suggested that bioaerosols were generated mainly from the mixed liquor and will be promoted by the mechanical motion of belts of dewatering devices. they will accumulate in the sdhs due to ...201829136598
bioconversion of α-chitin into n-acetyl-glucosamine using chitinases produced by marine-derived aeromonas caviae isolates.n-acetyl-d-glucosamine (glcnac) is a monosaccharide with great application potential in the food, cosmetic, pharmaceutical, and biomaterial areas. glcnac is currently produced by chemical hydrolysis of chitin, but the current processes are environmentally unfriendly, have low yield and high cost. this study demonstrates the potential to produce glcnac from α-chitin using chitinases of ten marine-derived aeromonas isolates as a sustainable alternative to the current chemical process. the isolates ...201729080074
xanthine oxidase activity affects pro-oxidative and pro-inflammatory profiles in spleen of silver catfish experimentally infected with aeromonas caviae.several pieces of evidence have linked the involvement of xanthine oxidase (xo), a source of uric acid and reactive oxygen species (ros), to pro-oxidative and pro-inflammatory effects observed during bacterial fish diseases. thus, the aim of this study was to evaluate whether upregulation of splenic xo activity contributes to disease pathogenesis of aeromonas caviae infection, as well as whether it may be considered a pathway involved in ros and nitric oxide (no) production. xo activity increase ...201729042304
retraction notice: isolation and characterization of an aeromonas punctata bacteriophage.[this retracts the article on p. 185 in vol. 9.].201628932327
sewage treatment effluents in delhi: a key contributor of β-lactam resistant bacteria and genes to the environment.rapid emergence of antibiotic resistance (ar) in developing countries is posing a greater health risk and increasing the global disease burden. lack of access to safe drinking water, poor sanitation and inadequate sewage treatment facilities in these countries are fueling the problem associated with emergence of ar. rapid proliferation of ar mediated by treated and untreated discharges from sewage treatment plants (stps) is a prime public health concern. this study aims to understand the occurre ...201728886559
first report of blaoxa-181 mediated carbapenem resistance in aeromonas caviae in association with pkp3-a: threat for rapid dissemination.aeromonas spp producing carbapenemases is of great concern in health-care settings and also known to acquire clinically relevant resistance genes. in this study, carbapenem non-susceptible aeromonas isolates were characterized for their molecular mechanism.201728743649
prevalence and characterisation of quinolone resistance genes in aeromonas spp. isolated from pet turtles in south korea.the aim of this study was to characterise aeromonas spp. isolated from popular species of pet turtle to assess the potential risk of pet turtles as a source of target gene alterations in the quinolone resistance-determining region (qrdr) and transferable plasmid-mediated quinolone resistance (pmqr) genes.201728743647
aeromonas caviae alters the cytosolic and mitochondrial creatine kinase activities in experimentally infected silver catfish: impairment on renal bioenergetics.cytosolic and mitochondrial creatine kinases (ck), through the creatine kinase-phosphocreatine (ck/pcr) system, provide a temporal and spatial energy buffer to maintain cellular energy homeostasis. however, the effects of bacterial infections on the kidney remain poorly understood and are limited only to histopathological analyses. thus, the aim of this study was to investigate the involvement of cytosolic and mitochondrial ck activities in renal energetic homeostasis in silver catfish experimen ...201728735082
isolation and characterization of an aeromonas punctata bacteriophage.an aeromonas punctata bacteriophage, named as dh1, was isolated from east lake, wuhan city, china. morphologically, phage dh1 showed a typical myoviridae structure consisting of an isometric head (50 nm in diameter) and a visible tail. the bacteriophage had a latent period of about 90 minutes and an average burst size of about 125 pfu•cell(-1). restriction enzyme pattern of the bacteriophage's genome showed that the genome is a double-stranded dna and about 34kb in size. the sequenced genomic fr ...201528484577
identification of bacteria in blood cultures from clinically ill captive antillean manatees (trichechus manatus manatus).between september 2001 and march 2013, 62 bacterial cultures (37 aerobic and 25 anaerobic) were performed on 37 blood samples from 23 antillean manatees ( trichechus manatus manatus) that were kept in captivity at the brazilian national center for research and conservation of aquatic mammals (cma) in pernambuco (cma-pe) and alagoas (cma-al), brazil. all of the animals sampled exhibited clinical signs at the time of sampling including abscesses (n = 8), debilitation and anorexia (n = 22), and pro ...201728363079
incidence and virulence characteristics of aeromonas spp. in fish.this study was conducted to evaluate the presence of aeromonas spp. in raw and ready-to-eat (rte) fish commonly consumed in assiut city, egypt, and to determine virulence factors due to they play a key role in their pathogenicity.201728246446
butyrate production under aerobic growth conditions by engineered escherichia coli.butyrate is an important industrial platform chemical. although several groups have reported butyrate production under oxygen-limited conditions by a native producer, clostridium tyrobutylicum, and by a metabolically engineered escherichia coli, efforts to produce butyrate under aerobic growth conditions have met limited success. here, we constructed a novel butyrate synthetic pathway that functions under aerobic growth conditions in e. coli, by modifying the 1-butanol synthetic pathway reported ...201728089378
clinical and therapeutic implications of aeromonas bacteremia: 14 years nation-wide experiences in korea.to elucidate the clinical presentation, antimicrobial susceptibility, and prognostic factors of monomicrobial aeromonas bacteremia in order to determine the most effective optimal therapy.201628032485
draft genome sequence of aeromonas caviae ch129, a marine-derived bacterium isolated from the coast of são paulo state, brazil.we report here the draft genome sequence of aeromonas caviae ch129, a marine-derived bacterium isolated from the coast of são paulo state, brazil. genomic analysis revealed genes encoding enzymes involved in binding, transport, and chitin metabolism and different virulence-associated factors.201627908996
draft genome sequence of marine-derived aeromonas caviae chz306, a potential chitinase producer strain.we report here a draft genome sequence of aeromonas caviae chz306, a marine-derived bacterium with the ability to hydrolyze chitin and express high levels of chitinases. the assembly resulted in 65 scaffolds with approximately 4.78 mb. genomic analysis revealed different genes encoding chitin-degrading enzymes that can be used for chitin derivative production.201627856589
production, characterization, and immobilization of partially purified surfactant-detergent and alkali-thermostable protease from newly isolated aeromonas caviae.proteolytic aeromonas caviae p-1-1 growing at wide-ranging ph (7.0-11.0) and moderate salinity (0-5% nacl) was isolated from cattle shed of thanjavur, india. it produced lipase, gelatinase, and polyhydroxybutyrate. different culture conditions, incubation time, carbon and nitrogen sources, vitamins, amino acids, surfactants, and metal ions for optimal growth and protease production of p-1-1 were examined. maximum protease (0.128 u/ml) production was achieved with 1% fructose, 1% yeast extract, 0 ...201627737605
reassessment of the enteropathogenicity of mesophilic aeromonas species.cases of aeromonas diarrhea have been described all over the world. the genus aeromonas includes ca. 30 species, of which 10 have been isolated in association with gastroenteritis. the dominating species that account for ca. 96% of the identified strains are aeromonas caviae, a. veronii, a. dhakensis, and a. hydrophila. however, the role of aeromonas as a true enteropathogen has been questioned on the basis of the lack of outbreaks, the non-fulfillment of koch's postulates and the low numbers of ...201627708621
prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in messalata central hospital, libya.to investigate the prevalence of urinary tract infection among patients at messalata central hospital, libya, to identify the causative bacteria, and to explore their resistance pattern to antimicrobials.201627569886
adhesion and cytotoxicity of aeromonas caviae to rabbit intestinal epithelium ex vivo.aeromonads are considered potential pathogens for humans and animals and are responsible for the etiology of intestinal and extraintestinal diseases. the presence of aeromonas spp. in food and water shows that it is an important vehicle of infection in humans. the pathology caused by these bacteria involves several virulence factors, such as the ability to produce toxins, adhesion and invasion. the present study investigated the interaction of five aeromonas caviae strains isolated from human di ...201627394162
in azospirillum brasilense, mutations in flma or flmb genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots.azospirillum brasilense is a soil bacterium capable of promoting plant growth. several surface components were previously reported to be involved in the attachment of a. brasilense to root plants. among these components are the exopolysaccharide (eps), lipopolysaccharide (lps) and the polar flagellum. flagellin from polar flagellum is glycosylated and it was suggested that genes involved in such a posttranslational modification are the same ones involved in the biosynthesis of sugars present in ...201627393999
production of biodegradable polyester by a transgenic tobacco.the acetoacetyl-coa reductase gene (phbb) of ralstonia eutropha and the poly[(r)-(-)-3-hydroxyalkanoate] synthase gene (phacac) of aeromonas caviae were introduced into tobacco plant by agrobacterium mediated transformation method. the resulting transgenic tobacco expressed both introduced genes and the expression of these genes was confirmed by enzymatic analysis and western blotting. gc-ms analysis of the chloroform extract of tobacco leaves indicated that the transgenic plant produced biodegr ...199927385570
evaluation of different conditions and culture media for the recovery of aeromonas spp. from water and shellfish samples.to perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ada), starch ampicillin agar (saa), bile salts irgasan brilliant green modified (bibg-m)) for recovering aeromonas species from water and shellfish samples.201627333573
pan-genome analysis of aeromonas hydrophila, aeromonas veronii and aeromonas caviae indicates phylogenomic diversity and greater pathogenic potential for aeromonas hydrophila.aeromonas species are important pathogens of fishes and aquatic animals capable of infecting humans and other animals via food. due to the paucity of pan-genomic studies on aeromonads, the present study was undertaken to analyse the pan-genome of three clinically important aeromonas species (a. hydrophila, a. veronii, a. caviae). results of pan-genome analysis revealed an open pan-genome for all three species with pan-genome sizes of 9181, 7214 and 6884 genes for a. hydrophila, a. veronii and a. ...201627075453
a study on the relation between poly(3-hydroxybutyrate) depolymerases or oligomer hydrolases and molecular weight of polyhydroxyalkanoates accumulating in cupriavidus necator h16.cupriavidus necator h16 has nine genes of poly(3-hydroxybutyrate) (phb) depolymerases or oligomer hydrolases (intracellular phb mobilization enzymes). in this study, we evaluated the relation between these genes and the accumulation, consumption, and molecular weight of polyhydroxyalkanoates (phas) accumulating in strain h16 and in a recombinant c. necator strain, knk-005, which harbors an nsdg mutant of the pha synthase gene (phacac) from aeromonas caviae. phaz6 had a significant influence on t ...201627059479
impact of stress on aeromonas diversity in tambaqui (colossoma macropomum) and lectin level change towards a bacterial challenge.tambaqui (colossoma macropomum) is among the most cultivated fish species in tropical countries. stress is the main cause of disease in fish farms. the genus aeromonas is a common causative agent of fish diseases. this work reports the identification of aeromonas species colonizing gills of c. macropomum submitted or not to a confinement stress. we also evaluated changes in serum levels of lectins (carbohydrate-binding proteins that are components of fish immune system) in tambaqui submitted to ...201627046480
[infection status and virulent genes of aeromonas in diarrhea patients in pudong new area, shanghai].to investigate the infection status and virulent genes of aeromonas in patients with acute diarrhea in pudong new area, shanghai.201627005546
unusual microorganisms and antimicrobial resistances in a group of syrian migrants: sentinel surveillance data from an asylum seekers centre in italy.three years of civil war in syria have caused death and increase of communicable diseases. the suffering population has been forced to migrate creating a fertile condition for epidemic spread of infection within the refugee camps.201726987764
genotypic and phenotypic identification of aeromonas species and cpha-mediated carbapenem resistance in queensland, australia.infection caused by aeromonas spp. ranges from superficial wound infection to life-threatening septicemia. carbapenem resistance due to metallo-beta-lactamase, cpha encoded by the cpha gene, is a significant problem. this study defines aeromonas spp. causing clinical disease in queensland, australia. phenotypic tests for carbapenemase detection were assessed. one hundred aeromonas isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2), and sputum (1) were characterized by ...201626971634
antibiogram characterization and putative virulence genes in aeromonas species isolated from pig fecal samples.aeromonas species are broadly distributed in nature and agricultural environments and have been isolated from feces, bedding, and drinking water of healthy pigs. we assessed the incidence, virulence properties, and antimicrobial resistance profile of aeromonas spp., isolated from pig feces. antibiogram was done using the disc diffusion methods, and polymerase chain reaction was used for the detection of putative virulence genes. identification of isolates revealed three phenotypic species with p ...201626971520
effect of antibiotic supplementation on the quality of cryopreserved fish sperm of silver barb (barbodes gonionotus): sperm motility and viability, bacterial quality and fertilization.disease prevention is a key aspect in developing cryopreservation procedures for fish sperm and in improving the reproductive biotechnology for commercially important aquatic species. the purpose of this study was to evaluate the effects of an antibiotic supplementation (0.25% penicillin-streptomycin, ps and 0.25% penicillin-gentamicin, pg) on sperm motility and viability, bacterial profile and fertilization capacity of cryopreserved silver barb (barbodes gonionotus) semen. the experimental prot ...201626786020
evidence of antibacterial activities in peptide fractions originating from snow crab (chionoecetes opilio) by-products.antibacterial peptide fractions generated via proteolytic processing of snow crab by-products exhibited activity against gram-negative and gram-positive bacteria. among the bacterial strains tested, peptide fractions demonstrated inhibitory activity against the gram-negative bacteria such as aeromonas caviae, aeromonas hydrophila, campylobacter jejuni, listonella anguillarum, morganella morganii, shewanella putrefasciens, vibrio parahaemolyticus and vibrio vulnificus and against a few gram-posit ...201026781242
characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein pham from ralstonia eutropha.the binding preference of a polyhydroxyalkanoate (pha) biosynthesis-related multifunctional protein from ralstonia eutropha (phamre) was characterized. in vitro activity assay showed that pha synthase from r. eutropha (phacre) was activated by the presence of phamre but pha synthase from aeromonas caviae (phacac) was not. additionally, in vitro assays of protein-protein interactions demonstrated that phamre interacted with phacre directly, but did not interact with phacac. these results suggest ...201626728018
characterisation of a chromosomally-encoded extended-spectrum β-lactamase gene blaper-3 in aeromonas caviae of chicken origin. 201626674256
availability of safe drinking-water: the answer to cholera outbreak? nabua, camarines sur, philippines, 2012.in may 2012, there were increasing diarrhoea cases and deaths reported from nabua, camarines sur to the philippines event-based surveillance system. an investigation was conducted to identify risk factors and determine transmission dynamics.201526668761
compositional regulation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by replacement of granule-associated protein in ralstonia eutropha.phasin (phap), a kind of polyhydroxyalkanoate (pha) granule-associated proteins, has a role in controlling the properties of pha granules surface, and is thought to have influence on pha biosynthesis in pha-producing bacteria. this study focused on the phap1(re) locus in ralstonia eutropha as a site of chromosomal modification for production of flexible poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] from soybean oil.201526597300
quinolone resistant aeromonas spp. as carriers and potential tracers of acquired antibiotic resistance in hospital and municipal wastewater.members of the genus aeromonas are recognized carriers of antibiotic resistance in aquatic environments. however, their importance on the spread of resistance from hospital effluents to the environment is poorly understood. quinolone resistant aeromonas spp. (n = 112) isolated from hospital effluent (he) and from raw (rww) and treated wastewater (tww) of the receiving urban wastewater treatment plant (uwtp) were characterized. species identification and genetic intraspecies diversity were assess ...201626546762
analysis of the structure and function of fox-4 cephamycinase.class c β-lactamases poorly hydrolyze cephamycins (e.g., cefoxitin, cefotetan, and moxalactam). in the past 2 decades, a new family of plasmid-based ampc β-lactamases conferring resistance to cefoxitin, the fox family, has grown to include nine unique members descended from the aeromonas caviae chromosomal ampc. to understand the basis for the unique cephamycinase activity in the fox family, we determined the first x-ray crystal structures of fox-4, apo enzyme and the acyl-enzyme with its namesa ...201626525784
draft genome sequence of aeromonas caviae strain 429865 inp, isolated from a mexican patient.aeromonas caviae is an emerging human pathogen. here, we report the draft genome sequence of aeromonas caviae strain 429865 inp which shows the presence of various putative virulence-related genes.201526494682
diarrhea outbreak in pernambuco, brazil, associated with a heat-stable cytotoxic enterotoxin produced by aeromonas caviae.in the present study enterotoxic and cytotoxic activities of twenty aeromonas caviae strains were examined. they originated from fecal specimens of patients with acute diarrhea during an outbreak in brazil in 2004. culture supernatants of fourteen strains (70%) caused fluid accumulation in rabbit ileal intestinal loops and in suckling mice assays, and also showed a cytotoxic activity in vero and caco-2 cells. the enterotoxic and cytotoxic factors were heat-stable after culture supernatants treat ...201526422161
newborn bacteraemia caused by an aeromonas caviae producing the vim-1 and shv-12 β-lactamases, encoded by a transferable plasmid. 201626410172
comparative analysis of virulence genes, antibiotic resistance and gyrb-based phylogeny of motile aeromonas species isolates from nile tilapia and domestic fowl.the nucleotide sequence analysis of the gyrb gene indicated that the fish aeromonas spp. isolates could be identified as aeromonas hydrophila and aeromonas veronii biovar sobria, whereas chicken aeromonas spp. isolates identified as aeromonas caviae. pcr data revealed the presence of lip, ser, aer, act and cai genes in fish aer. hydrophila isolates, act, cai and aer genes in fish aer. veronii bv sobria isolates and ser and cai genes in chicken aer. caviae isolates. all chicken isolates showed va ...201526280543
development of a pcr assay based on a single-base pair substitution for the detection of aeromonas caviae by targeting the gyrb gene.aeromonas caviae is a bacterial pathogen that causes various infectious diseases in both humans and animals. to facilitate its detection, we developed species-specific primer sets targeting polymorphisms in the gyrb gene for use in a pcr assay. the technique was able to detect 100% (29/29) of the a. caviae strains tested using either of two sets of primers (designated acf1-acr and acf3-acr), which produced 293-bp and 206-bp amplicons, respectively. another set of primers (designated acf2-acr) yi ...201526223267
virulence and antimicrobial susceptibility of clinical and environmental strains of aeromonas spp. from northeastern brazil.the aims of the present study were to isolate and identify clinical and environmental strains of aeromonas spp. by means of biochemical tests and the automated method vitek 2 and to investigate the presence of the virulence genes cytotoxic enterotoxin (act), hemolysin (asa-1), and type iii secretion system (ascv), and also the in vitro antimicrobial susceptibility of the strains. from the clinical isolates, 19 aeromonas hydrophila, 3 aeromonas veronii bv. sobria, and 1 aeromonas caviae were iden ...201526103449
aeromonas punctata derived depolymerase improves susceptibility of klebsiella pneumoniae biofilm to gentamicin.to overcome antibiotic resistance in biofilms, enzymes aimed at biofilm dispersal are under investigation. in the present study, applicability of an aeromonas punctata derived depolymerase capable of degrading the capsular polysaccharide (cps) of klebsiella pneumoniae, in disrupting its biofilm and increasing gentamicin efficacy against biofilm was investigated.201526063052
occurrence of bacteriophages infecting aeromonas, enterobacter, and klebsiella in water and association with contamination sources in thailand.the co-residence of bacteriophages and their bacterial hosts in humans, animals, and environmental sources directed the use of bacteriophages to track the origins of the pathogenic bacteria that can be found in contaminated water. the objective of this study was to enumerate bacteriophages of aeromonas caviae (aecaks148), enterobacter sp. (enspks513), and klebsiella pneumoniae (klpnks648) in water and evaluate their association with contamination sources (human vs. animals). bacterial host strai ...201526042992
[pylephlebitis due to aeromonas caviae secondary to acute cholecystitis]. 201626031554
[the relevance of correct identification and interpretation of susceptibility testing of aeromonas spp. bacteremia isolates].to assess the relevance of correct identification and interpretation of susceptibility testing of aeromonas spp. bacteremia isolates using newly developed molecular methods in comparison to previous conventional methods.201626027900
a single-nucleotide substitution in phasin gene leads to enhanced accumulation of polyhydroxyalkanoate (pha) in escherichia coli harboring aeromonas caviae pha biosynthetic operon. 201526018503
draft genome sequence of aeromonas caviae 8lm, isolated from stool culture of a child with diarrhea.aeromonas spp. are gram-negative rods ubiquitous in aquatic environments; however, some species are able to cause a variety of infections in humans. here, we report the draft genome sequence of aeromonas caviae 8lm isolated from stool culture from a child with diarrhea in southern brazil.201525999559
construction of coa-dependent 1-butanol synthetic pathway functions under aerobic conditions in escherichia coli.1-butanol is an important industrial platform chemical and an advanced biofuel. while various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success. here, we constructed a coa-dependent 1-butanol synthetic pathway that functions under aerobic conditions in escherichia coli, by expanding the previously reported (r)-1,3-butanediol synthetic pathway. the pathway consists of ...201525865277
spontaneous bacterial peritonitis caused by aeromonas caviae in a patient with cirrhosis.spontaneous bacterial peritonitis (sbp) is a common complication of cirrhosis. based on our current understanding of sbp, the most common etiologies for sbp in cirrhosis are enterobacter and streptococcal species. th e aeromonas species are ubiquitous in fresh or sea water. aeromonas caviae is never identified as etiology in cases of sbp. a patient, who had a history of liver cirrhosis related to chronic hepatitis b virus infection for 1 year, presented with diarrhea. he had diarrhea 1 week late ...201525832540
regulation of 3-hydroxyhexanoate composition in phbh synthesized by recombinant cupriavidus necator h16 from plant oil by using butyrate as a co-substrate.a (r)-3-hydroxyhexanoate (3hh) composition-regulating technology for poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbh) production was developed using recombinant cupriavidus necator h16 with butyrate as a co-substrate. a new (r)-3-hydroxyhexanoyl-coa ((r)-3hh-coa) synthesis pathway was designed and enhanced by replacing the pha synthase gene (phac1) of c. necator by the phacacnsdg (encoding the n149s and d171g mutant of pha synthase from aeromonas caviae) and deactivation of the phaa gene (e ...201525805434
antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia).antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ...201525803299
draft genome sequence of aeromonas caviae strain l12, a quorum-sensing strain isolated from a freshwater lake in malaysia.here, we present the draft genome sequence of aeromonas caviae strain l12, which shows quorum-sensing activity. the availability of this genome sequence is important to the research of the quorum-sensing regulatory system in this isolate.201525745006
occurrence, molecular characterization, and antimicrobial susceptibility of aeromonas spp. in marine species of shrimps cultured at inland low salinity ponds.we aimed to document the risk of aeromonas spp. in marine shrimp species cultured in inland low salinity ponds in thailand. in 14 of 18 shrimp samples retrieved from inland grow-up ponds, aeromonas spp. were detected at ranges from 4667 to 1,500,000 cfu/g body weight. the phylogenetic tree constructed with the gyrb and cpn60 concatenated sequences indicated that the 87 isolates consisted of aeromonas veronii (70%), aeromonas aquariorum (18%), aeromonas caviae (7%), aeromonas jandaei (2%), and ae ...201525583334
the aeromonas caviae aha0618 gene modulates cell length and influences swimming and swarming motility.aeromonas caviae is motile via a polar flagellum in liquid culture, with a lateral flagella system used for swarming on solid surfaces. the polar flagellum also has a role in cellular adherence and biofilm formation. the two subunits of the polar flagellum, flaa and flab, are posttranslationally modified by o-linked glycosylation with pseudaminic acid on 6-8 serine and threonine residues within the central region of these proteins. this modification is essential for the formation of the flagellu ...201425515520
population dynamics and antimicrobial susceptibility of aeromonas spp. along a salinity gradient in an urban estuary in northeastern brazil.the main objective of this study was to quantify population and identify culturable species of aeromonas in sediment and surface water collected along a salinity gradient in an urban estuary in northeastern brazil. thirty sediment samples and 30 water samples were collected from 3 sampling locations (a, b and c) between october 2007 and april 2008. the aeromonas count was 10-7050cfu/ml (a), 25-38,500cfu/ml (b) and<10cfu/ml (c) for water samples, and ∼100-37,500cfu/g (a), 1200-43,500cfu/g (b) and ...201425455376
distribution, virulence-associated genes and antimicrobial resistance of aeromonas isolates from diarrheal patients and water, china.to determine the prevalence of aeromonas infections in diarrheal patients, the distribution of virulence-associated genes and antibiotic resistance among different aeromonas species in china.201525447712
aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland.we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ...201525380369
expression of aeromonas punctata me-1 exo-xylanase x in e. coli for efficient hydrolysis of xylan to xylose.exo-xylanase x from aeromonas punctata me-1 was functionally expressed in escherichia coli with a carboxy terminal his tag (6×) and a molecular mass of 39.42 kda, which is in agreement with the prediction from its amino acid composition. the recombinant exo-xylanase reached 186 mg l(-1) after induction by isopropyl β-d-1-thiogalactopyranoside. its optimal temperature and ph were 50 °c and 6, respectively. the enzyme showed not only an exo-xylanase activity with k m of 3.90 mg ml(-1) and v max of ...201425213085
depolymerase improves gentamicin efficacy during klebsiella pneumoniae induced murine infection.presence of capsule enhances the virulence of bacteria that cause pneumonia, meningitis, cystic fibrosis, dental caries, periodontitis. capsule is an important virulence factor for klebsiella pneumoniae and infections due to this pathogen have been associated with high mortality rates. in the present study, use of an aeromonas punctata derived capsule depolymerase against k. pneumoniae, to reinstate the efficacy of gentamicin during pneumonia and septicemia was investigated.201425149315
quorum sensing activity of aeromonas caviae strain yl12, a bacterium isolated from compost.quorum sensing is a well-studied cell-to-cell communication method that involves a cell-density dependent regulation of genes expression mediated by signalling molecules. in this study, a bacterium isolated from a plant material compost pile was found to possess quorum sensing activity based on bioassay screening. isolate yl12 was identified using matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry and molecular typing using rpod gene which identified the iso ...201424759107
on the mechanism underlying tellurite reduction by aeromonas caviae st dihydrolipoamide dehydrogenase.the dihydrolipoamide dehydrogenase (lpda) from the tellurite-resistant bacterium aeromonas caviae st reduces tellurite to elemental tellurium. to characterize this nadh-dependent activity, the a. caviae lpda gene was subjected to site-directed mutagenesis and genes containing c45a, h322y and e354k substitutions were individually transformed into escherichia coli δlpd. cells expressing the modified genes exhibited decreased pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and tr activity re ...201424680738
phasin proteins activate aeromonas caviae polyhydroxyalkanoate (pha) synthase but not ralstonia eutropha pha synthase.in this study, we performed in vitro and in vivo activity assays of polyhydroxyalkanoate (pha) synthases (phacs) in the presence of phasin proteins (phaps), which revealed that phaps are activators of phac derived from aeromonas caviae (phacac). in in vitro assays, among the three phacs tested, phacac was significantly activated when phaps were added at the beginning of polymerization (prepolymerization phacac), whereas the prepolymerization phacre (derived from ralstonia eutropha) and phacda (d ...201424584238
a neonate with a meningomyelocele complicated by aeromonas caviae ventriculoperitoneal shunt infection.we report on a newborn girl with a aeromonas caviae shunt infection and meningitis after insertion of a ventriculoperitoneal shunt and surgical repair of a meningomyelocele in one procedure. this pathogen has never been reported, related to ventriculoperitoneal shunt infections. beside the need for surgical revision of the shunt because of shunt obstruction and septa formation in the ventricles, the clinical outcome was good with intravenous cefotaxime therapy.201624579246
maf-dependent bacterial flagellin glycosylation occurs before chaperone binding and flagellar t3ss export.bacterial swimming is mediated by rotation of a filament that is assembled via polymerization of flagellin monomers after secretion via a dedicated flagellar type iii secretion system. several bacteria decorate their flagellin with sialic acid related sugars that is essential for motility. aeromonas caviae is a model organism for this process as it contains a genetically simple glycosylation system and decorates its flagellin with pseudaminic acid (pse). the link between flagellin glycosylation ...201424527847
biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) by recombinant escherichia coli expressing leucine metabolism-related enzymes derived from clostridium difficile.an obligate anaerobic bacterium clostridium difficile has a unique metabolic pathway to convert leucine to 4-methylvalerate, in which 4-methyl-2-pentenoyl-coa (4m2pe-coa) is an intermediate of this pathway. 4m2pe-coa is also able to be converted to 3-hydroxy-4-methylvalerate (3h4mv), a branched side chain monomer unit, for synthesis of polyhydroxyalkanoate (pha) copolymer. in this study, to synthesize 3h4mv-containing pha copolymer from leucine, the leucine metabolism-related enzymes (ldha and h ...201424484910
biosynthesis of polyhydroxyalkanoate copolymers from methanol by methylobacterium extorquens am1 and the engineered strains under cobalt-deficient conditions.methylobacterium extorquens am1 has been shown to accumulate polyhydroxyalkanoate (pha) composed solely of (r)-3-hydroxybutyrate (3hb) during methylotrophic growth. the present study demonstrated that the wild-type strain am1 grown under co²⁺-deficient conditions accumulated copolyesters of 3hb and a c₅-monomer, (r)-3-hydroxyvalerate (3hv), using methanol as the sole carbon source. the 3hv unit was supposed to be derived from propionyl-coa, synthesized via the ethylmalonyl-coa pathway impaired b ...201424430207
molecular cloning and characterization of a toll receptor gene from macrobrachium rosenbergii.toll receptors are cell surface molecules acting as pattern recognition receptors (prrs) that have been implicated in the signaling pathway of innate immune responses. in this study, the full-length cdna of a toll receptor gene of macrobrachium rosenbergii, designated mrtoll, was successfully isolated using designed degenerate primers and the rapid amplification of cdna ends (race). the mrtoll gene sequence contained an open reading frame (orf) of 2799 nucleotides encoding a protein of 932 amino ...201424398262
emergence of vim-producing aeromonas caviae in israeli hospitals.resistance to carbapenems in aeromonas species is rare and mediated mostly by the chromosomal cpha gene. our aims were to describe the molecular characteristics of the first cases of vim-producing aeromonas caviae isolated from human samples.201424390932
cholera-like illness due to aeromonas caviae. 201324222288
seasonal incidence of and antibiotic resistance among aeromonas species isolated from domestic wastewater before and after treatment in stabilization ponds.the efficiency of stabilization pond treatment of domestic wastewater in removing culturable cells of motile aeromonas and its influence on the incidence of resistance to seven antibiotics were investigated in this study. removal efficiency was higher (p < 0.001) in the warm months (98.8%) than in the cold months (97%). among the 264 isolates, 163 were aeromonas caviae, 24 were a. hydrophila, and 54 were a. sobria. twenty-three isolates could not be identified to the species level. in the influe ...199224192933
the in-vitro antibacterial effects of organic salts, chemical disinfectants and antibiotics against pathogens of black disease in fairy shrimp of thailand.the antibacterial effects of organic salts, chemical disinfectants and antibiotics were evaluated on cultures of aeromonas hydrophila c03, aeromonas sobria c26, a. sobria c29, aeromonas caviae c24 and acinetobacter sp. sh-94b, the pathogens that cause black disease found in fairy shrimps (streptocephalus sirindhornae sanoamuang et al. (2000) and branchinella thailandensis sanoamuang, saengphan & murugan) of thailand. the minimal inhibitory concentrations (mics) of organic salts (sodium chloride ...201424131294
aqu-1, a chromosomal class c β-lactamase, among clinical aeromonas dhakensis isolates: distribution and clinical significance.aeromonas dhakensis, a recently described aeromonas sp. formerly called aeromonas aquariorum, is associated with human infections. in this study, a chromosomal gene, blaaqu-1, was identified in a. dhakensis aak1 that constitutes a 1143-bp open reading frame and is 87% identical to the gene encoding ceph in aeromonas hydrophila. an escherichia coli top10 cell transformant harbouring blaaqu-1 was resistant to cefotaxime but not to cefepime. mrna expression of blaaqu-1 in the cefotaxime-resistant m ...201324055254
virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health.aeromonas spp. are ubiquitous aquatic organisms, associated with multitude of diseases in several species of animals, including fishes and humans. in the present study, water samples from two ornamental fish culture systems were analyzed for the presence of aeromonas. nutrient agar was used for aeromonas isolation, and colonies (60 no) were identified through biochemical characterization. seven clusters could be generated based on phenotypic characters, analyzed by the programme ntsyspc, version ...201224031887
the occurrence of aeromonas in drinking water, tap water and the porsuk river.the occurrence of aeromonas spp. in the porsuk river, public drinking water and tap water in the city of eskisehir (turkey) was monitored. fresh water samples were collected from several sampling sites during a period of one year. total 102 typical colonies of aeromonas spp. were submitted to biochemical tests for species differentiation and of 60 isolates were confirmed by biochemical tests. further identifications of isolates were carried out first with the vitek system (biome˜rieux) and then ...201124031613
characterization of aeromonas species isolated from an estuarine environment.thirty water samples were collected, at two week intervals, from the estuary of the river cocó. the aim was to characterize the presence, distribution and types of aeromonas spp, in the estuary of the river cocó, ceara, brazil (03°46'28.83''s e 38°26'36.52''s). aeromonas were identified in 19 (63%) samples analyzed by plating and cfu counts. presence/absence tests were positive for 11 (37%) of the samples resulting in the detection of aeromonas in a total of 23 (77%) of samples. cfu counts varie ...201024031516
lectin-binding properties of aeromonas caviae strains.the cell surface carbohydrates of four strains of aeromonas caviae were analyzed by agglutination and lectin-binding assays employing twenty highly purified lectins encompassing all sugar specificities. with the exception of l-fucose and sialic acid, the sugar residues were detected in a. caviae strains. a marked difference, however, in the pattern of cell surface carbohydrates in different a. caviae isolates was observed. specific receptors for tritricum vulgaris (wga), lycopersicon esculentum ...200824031204
modification of β-oxidation pathway in ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil.ralstonia eutropha h16 is a useful platform for metabolic engineering aiming at efficient production of polyhydroxyalkanaotes being attracted as practical bioplastics. this study focused on bifunctional (s)-specific 2-enoyl-coa hydratase/(s)-3-hydroxyacyl-coa dehydrogenase encoded by fadb to obtain information regarding β-oxidation in this bacterium and to achieve compositional regulation of poly((r)-3-hydroxybutyrate-co-(r)-3-hydroxyhexanoate) [p(3hb-co-3hhx)] synthesized from soybean oil. in a ...201423999062
aeromonas punctata derived depolymerase that disrupts the integrity of klebsiella pneumoniae capsule: optimization of depolymerase production.formation of dense, highly hydrated biofilm structures pose a risk for public and environmental health. extracellular polymeric substances encompassing biofilms offer 1000-fold greater resistance as compared to the planktonic cells. using enzymes as anti-biofouling agents, will improve penetration of antimicrobials and increase susceptibility of biofilms to components of immune system. the challenge of using enzymes derived from unrelated bacteria for the degradation of capsular matrix of klebsi ...201423996303
aeromonas spp. simultaneously harbouring bla(ctx-m-15), bla(shv-12), bla(per-1) and bla(fox-2), in wild-growing mediterranean mussel (mytilus galloprovincialis) from adriatic sea, croatia.aeromonas species are becoming renowned as emerging pathogens by increasingly giving rise to a wide spectrum of food and waterborne infections in humans. another worrisome feature of aeromonads is the growing frequency of antibiotic resistance as a consequence of their prominent diversity in terms of resistance determinants. this study aimed at determining the antimicrobial resistance pattern, prevalence and characterization of acquired β-lactamases, including extended-spectrum-β-lactamases (esb ...201323973842
necrotizing fasciitis caused by aeromonas caviae.aeromonads are rarely associated with human intestinal and extra-intestinal diseases and syndromes, ranging from relatively mild illnesses such as acute gastroenteritis to life-threatening conditions, including septicemia, necrotizing fasciitis, and myonecrosis. among the aeromonas species known to cause human infection, aeromonas caviae has been associated with septicemia and only one reported case of human soft tissue infection. most of the infections due to aeromonas occur in immunocompromise ...201223826556
re-identification of aeromonas isolates from chironomid egg masses as the potential pathogenic bacteria aeromonas aquariorum.egg masses of the non-biting midge chironomous sp. have recently been found to serve as a reservoir for vibrio cholerae and aeromonas species. these insects are widely distributed in freshwater and evidence suggests that they may disseminate pathogenic bacteria species into drinking water systems. in the current study the taxonomy of 26 aeromonas isolates, previously recovered from chironomid egg masses, was re-evaluated. it was found that 23 isolates, which had previously been identified as aer ...201123761256
reclassification of aeromonas hydrophila subspecies anaerogenes.technological advances together with the continuous description of new taxa have led to frequent reclassifications in bacterial taxonomy. in this study, an extensive bibliographic revision, as well as a sequence analysis of nine housekeeping genes (cpn60, dnaj, dnax, gyra, gyrb, mdh, reca, rpob and rpod) and a phenotypic identification of aeromonas hydrophila subspecies anaerogenes were performed. all data obtained from previous physiological, phylogenetic, and dna-dna hybridization studies toge ...201323759598
molecular characterization of aeromonas species isolated from farmed eels (anguilla japonica).seventy aeromonas strains were identified by phylogenetic analysis using housekeeping genes (gyrb and rpod) in order to investigate etiological agents for aeromoniasis in farmed eels (anguilla japonica). the phylogenetic analysis showed that aeromonas aquariorum (n=22, 31.4%) was the predominant species among the investigated eel strains, followed by aeromonas caviae (n=16, 22.9%), a. veronii (n=13, 18.6%), a. hydrophila (n=12, 17.1%), a. jandaei (n=4, 5.7%), a. media (n=2, 2.9%), and a. trota ( ...201323499189
in vitro evidence of chain transfer to tetraethylene glycols in enzymatic polymerization of polyhydroxyalkanoate.a polyhydroxyalkanoate (pha) was enzymatically synthesized in vitro, and the end structure of pha associated with a chain transfer (ct) reaction was investigated. in the ct reaction, pha chain transfers from pha synthase (phac) to a ct agent, resulting in covalent bonding of ct agent to the pha chain at its carboxyl end. in vitro ct reaction has never been demonstrated because of relatively low yields of in vitro synthesized poly[(r)-3-hydroxybutyrate)] (p(3hb)), which makes it difficult to char ...201323474615
aeromonas caviae is the most frequent pathogen amongst cases of aeromonas bacteremia in japan.aeromonas species can cause various infections including bacteremia, gastroenteritis, cholangitis, and wound infections. to date, most studies on aeromonas species have been reported from countries other than japan. the aim of this study, therefore, was to evaluate aeromonas bacteremia in japan.201323163893
isolation and identification of aeromonas caviae strain ks-1 as tbtc- and lead-resistant estuarine bacteria.tributyltin chloride (tbtc)- and lead-resistant estuarine bacterium from mandovi estuary, goa, india was isolated and identified as aeromonas caviae strain ks-1 based on biochemical characteristics and fame analysis. it tolerates tbtc and lead up to 1.0 and 1.4 mm, respectively, in the minimal salt medium (msm) supplemented with 0.4 % glucose. scanning electron microscopy clearly revealed a unique morphological pattern in the form of long inter-connected chains of bacterial cells on exposure to ...201323132753
active intermediates of polyhydroxyalkanoate synthase from aeromonas caviae in polymerization reaction.polyhydroxyalkanoate (pha) synthase from aeromonas caviae fa440 (phac(ac), baa21815) is one of the most valuable pha synthase, because of its function to synthesize a practical bioplastic, poly[(r)-3-hydroxybutyrate-co-(r)-3-hydroxyhexanoate] [p(3hb-co-3hhx)]. however, biochemical activity and active intermediates of phac(ac) have not been clarified until now. in the present study, a gene of phac(ac) was cloned and overexpressed by a cell-free protein expression system. both the polymerization a ...201223043466
chironomid egg masses harbour the clinical species aeromonas taiwanensis and aeromonas sanarellii.bacteria of the genus aeromonas are found worldwide in aquatic environments and may produce human infections. in 2010, two new clinical species, aeromonas sanarellii and aeromonas taiwanensis, were described on the basis of one strain recovered from wounds of hospitalized patients in taiwan. so far, only four environmental isolates of a. sanarellii and one of a. taiwanensis have been recorded from waste water in portugal and an additional clinical strain of a. taiwanensis from the faeces of a pa ...201222967097
identification of a putative glycosyltransferase responsible for the transfer of pseudaminic acid onto the polar flagellin of aeromonas caviae sch3n.motility in aeromonas caviae, in a liquid environment (in broth culture), is mediated by a single polar flagellum encoded by the fla genes. the polar flagellum filament of a. caviae is composed of two flagellin subunits, flaa and flab, which undergo o-linked glycosylation with six to eight pseudaminic acid glycans linked to serine and threonine residues in their central region. the flm genetic locus in a. caviae is required for flagellin glycosylation and the addition of pseudaminic acid (pse) o ...201222950021
purification, characterization and amplification of a 1.8 kbp fragment of xylanase 5 from aeromonas caviae w-61.aeromonas caviae w-61 produces multiple extracellular xylanases, the xylanases 1, 2, 3, 4, and 5. in this study, we purified and characterized the xylanase 5 of a. caviae w-61, and amplified a part of xylanase 5 gene (xyn5). the purified xylanase 5 was found to be a single polypeptide with molecular mass of 140 kda. it was an endo-beta-1,4-xylanase showing optimum temperature 40 degrees c and optimum ph 6.0. xylobiose, xylotriose, xylotetrose, xylopentose, xylohexose and a small amount of xylose ...200222905388
molecular detection of the aeromonas virulence aerolysin gene in retail meats from different animal sources in egypt.meat commonly contain the same aeromonas spp. which occur in human diarrhoeal and non-diarrhoeal faecal samples. motile aeromonas were isolated from 5.6% of total 302 samples. the distribution of the isolates were 5.9 and 5.2% in fresh and frozen samples, respectively. of the 302 samples taken of the four animal meat species investigated, the genus aeromonas were isolated in 12.3% of the fresh samples collected from buffalo meat, in 6.5% of the samples collected from sheep meat and 14.0% from th ...201222806009
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