Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| strong genomic and phenotypic heterogeneity in the aeromonas sobria species complex. | aeromonas sobria is a mesophilic motile aeromonad currently depicted as an opportunistic pathogen, despite increasing evidence of mutualistic interactions in salmonid fish. however, the determinants of its host-microbe associations, either mutualistic or pathogenic, remain less understood than for other aeromonad species. on one side, there is an over-representation of pathogenic interactions in the a. sobria literature, of which only three articles to date report mutualistic interactions; on th ... | 2017 | 29276504 |
| involvement of the arg566 residue of aeromonas sobria serine protease in substrate specificity. | aeromonas sobria serine protease (asp) is an extracellular serine protease secreted by the organism. here, we identified the amino acid residue of asp that contributes to substrate specificity by using both synthetic peptides and biological protein components. the results showed that the arginine residue at position 566 (arg-566) of asp, which is located in the extra occluding region of asp close to an entrance of the catalytic cavity, is involved in the substrate specificity. a substitutional p ... | 2017 | 29023605 |
| secondary bacterial infections and antibiotic resistance among tungiasis patients in western, kenya. | tungiasis or jigger infestation is a parasitic disease caused by the female sand flea tunga penetrans. secondary infection of the lesions caused by this flea is common in endemic communities. this study sought to shed light on the bacterial pathogens causing secondary infections in tungiasis lesions and their susceptibility profiles to commonly prescribed antibiotics. participants were recruited with the help of community health workers. swabs were taken from lesions which showed signs of second ... | 2017 | 28886013 |
| oxa-427, a new plasmid-borne carbapenem-hydrolysing class d β-lactamase in enterobacteriaceae. | to describe a novel plasmid-borne class d carbapenemase (chdl) named oxa-427 identified in several enterobacteriaceae clinical isolates from nine patients in one belgian hospital. | 2017 | 28859446 |
| in vitro selection and identification of potential probiotics isolated from the gastrointestinal tract of nile tilapia, oreochromis niloticus. | fish gut bacteria can be used as probiotics for aquaculture. the aim of this study is to screen and identify beneficial probiotic bacteria from the gut of nile tilapia, oreochromis niloticus. nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, aeromonas sobria, aeromonas hydrophila, pseudomonas aeruginosa, pseudomonas putida, and staphylococcus aureus. fur ... | 2017 | 28819794 |
| curcumin liposomes interfere with quorum sensing system of aeromonas sobria and in silico analysis. | aeromonas sobria is opportunistic pathogen frequently found in environment and food. interfering with its quorum sensing (qs) system could be a promising way to alleviate its virulence. in this study, curcumin liposomes were prepared and their characteristics like particle size, zeta potential, pdi (polymey disperse index), encapsulation efficiency and loading capacity were measured. the quorum sensing inhibitory effect of curcumin liposomes under sub-mic (minimum inhibitory concentration) on si ... | 2017 | 28819178 |
| a truncated sph12-38 with potent antimicrobial activity showing resistance against bacterial challenge in oryzias melastigma. | antimicrobial peptides (amps) represent an efficient part of innate immunity and are found in a variety of life. among them histone 2a (h2a), as a promising class of amps, attracts great attention, but the in vivo mechanism of h2a derived amp is still less known. based on the acquisition of sphistin, a synthetic 38-amino acid h2a derived peptide from scylla paramamosain, as reported in our previous study, was truncated into three short fragments (sph12-38, sph20-38 and sph30-38) and further inve ... | 2017 | 28600196 |
| efficacy of spirulina platensis diet supplements on disease resistance and immune-related gene expression in cyprinus carpio l. exposed to herbicide atrazine. | the present study evaluated the immunotoxicological effects of the herbicide atrazine (atz) at sub-lethal concentrations and the potential ameliorative influence of spirulina platensis (sp) over a sub-chronic exposure period on cyprinus carpio l., also known as common carp. common carp was sampled after a 40-days exposure to atz (428 μg/l) and sp (1%), individually or in combination to assess the non-specific immune response, changes in mrna expression of immune-related genes [lysozyme (lyz), im ... | 2017 | 28579523 |
| aeromonas sobria, an outbreak of natural infection in cultured silver catfish rhamdia quelen (quoy & gaimard, 1824) in argentina. | 2017 | 28548705 | |
| hematopoietic neoplasms accompanied by severe enterocolitis due to aeromonas species. | aeromonas species are known to be a cause of diarrhea and acute enterocolitis. however, only a few cases have been reported and the pathophysiology of aeromonas infection has not as yet been clarified. we experienced 2 cases developing severe enterocolitis during the course of hematological malignancies, specifically multiple myeloma and diffuse large b-cell lymphoma. both patients presented with watery diarrhea that persisted for more than a week, followed by bloody diarrhea. total colon endosc ... | 2017 | 28484157 |
| aeromonas sobria serine protease (asp): a subtilisin family endopeptidase with multiple virulence activities. | aeromonas sobria serine protease (asp) is secreted from aeromonas sobria, a pathogen causing gastroenteritis and sepsis. asp resembles saccharomyces cerevisiae kex2, a member of the subtilisin family, and preferentially cleaves peptide bonds at the c-terminal side of paired basic amino acid residues; also accepting unpaired arginine at the p1 site. unlike kex2, however, asp lacks an intramolecular chaperone n-terminal propeptide, instead utilizes the external chaperone orf2 for proper folding, t ... | 2017 | 28432839 |
| determination of spoilage microbiota of pacific white shrimp during ambient and cold storage using next-generation sequencing and culture-dependent method. | this study was conducted to determine the initial and spoilage microbiota of pacific white shrimp during ambient and cold storage using next-generation sequencing (ngs) and a culture-dependent method. the quality changes were also evaluated by sensory analysis and total volatile basic nitrogen (tvb-n) values. after 1 d of storage, the psychrotrophic bacteria were only 5.97 log cfu/g, accounting for 1.1% of the mesophilic bacteria counts (7.94 log cfu/g). the psychrotrophic bacteria counts exceed ... | 2017 | 28399332 |
| ethmoiditis with subperiosteal and retro-ocular abscesses due to aeromonas sobria in a 16-year-old boy exposed to the ardèche river. | 2017 | 28343158 | |
| incidence and virulence characteristics of aeromonas spp. in fish. | this study was conducted to evaluate the presence of aeromonas spp. in raw and ready-to-eat (rte) fish commonly consumed in assiut city, egypt, and to determine virulence factors due to they play a key role in their pathogenicity. | 2017 | 28246446 |
| post-translationally modified frog skin-derived antimicrobial peptides are effective against aeromonas sobria. | antimicrobial peptides (brevinin1 hyba1 and brevinin1 hyba2) identified from the skin secretion of an endemic frog species of western ghats were studied against fish pathogens. post-translational modifications such as c-terminal amidation and cyclization of the peptides were enhanced on the activity against aeromonas sobria. based on the minimum inhibitory concentration (3 μm), cyclic amidated brevinin hyba2 was identified as the most promising antimicrobial agent against a. sobria and can be de ... | 2017 | 28153544 |
| draft genome sequence of aeromonas sobria strain 08005, isolated from sick rana catesbeiana. | aeromonas sobria is a gram-negative, rod-shaped, and ubiquitous bacterium. we present here the draft genome sequence of a. sobria strain 08005, isolated from an infected bullfrog. it is composed of 66 contigs totaling 4,678,951 bp, contains 4,252 coding dna sequences (cdss), four rrnas, and 88 trna sequences, and shows the presence of various putative virulence-related genes. | 2017 | 28082483 |
| antibacterial effect of gallic acid against aeromonas hydrophila and aeromonas sobria through damaging membrane integrity. | in the study, we investigated the antibacterial activity and mechanism of gallic acid against aeromonas hydrophila and aeromonas sobria. gallic acid showed strong antimicrobial activity against the two bacteria. furthermore, the antibacterial mechanism of gallic acid (0, 3, 6, 12 mm) was performed by membrane integrity assay and scanning electron microscopy (sem) assay. the results showed that gallic acid notably increased the released material absorption value at 260, 280 nm and electric conduc ... | 2017 | 27774889 |
| protective potency of clove oil and its transcriptional down-regulation of aeromonas sobria virulence genes in african catfish (clarias gariepinus l.). | disease episodes of fish caused by aeromonas species are moved to the top list of limiting problems worldwide. the present study was planned to verify the in vitro antibacterial activities as well as the in vivo potential values of clove oil and ciprofloxacin against aeromonas sobria in african catfish (clarias gariepinus). the in vitro phenotypic virulence activities and the successful amplification of aerolysin and hemolysin genes in the precisely identified a. sobria strain were predictive fo ... | 2016 | 27609474 |
| probiotic potential of autochthonous bacteria isolated from the gastrointestinal tract of four freshwater teleosts. | in this study, a total of 121 bacterial strains were isolated from the gastrointestinal tract of four teleostean species, namely striped snakehead (channa striatus), striped dwarf catfish (mystus vittatus), orangefin labeo (labeo calbasu) and mrigal carp (cirrhinus mrigala), among which 8 isolates showed promising antibacterial activity against four potential fish pathogens, aeromonas hydrophila, aeromonas salmonicida, aeromonas sobria and pseudomonas fluorescens and were non-hemolytic. the isol ... | 2017 | 27557836 |
| stress and humoral innate immune response of gilthead seabream sparus aurata cultured in sea cages. | innate and acquired immune responses of gilthead seabream sparus aurata was studied under normal culture and short-term stressful conditions for 18 months in offshore sea cages in alghero bay, italy. every 45 d, 50 fish were sampled and divided into two groups: fish in the first group (normal culture conditions) were bled after harvesting; fish in the second group were put into a tank under stressful conditions (crowding and confinement) and bled after 2 h. innate humoral immunity, such as compl ... | 2016 | 27485027 |
| involvement of acylated homoserine lactones (ahls) of aeromonas sobria in spoilage of refrigerated turbot (scophthalmus maximus l.). | one quorum sensing strain was isolated from spoiled turbot. the species was determined by 16s rrna gene analysis and classical tests, named aeromonas sobria as7. quorum-sensing (qs) signals (n-acyl homoserine lactones (ahls)) were detected by report strains and their structures were further determined by gc-ms. the activity changes of ahls on strain growth stage as well as the influence of different culture conditions on secretion activity of ahls were studied by the punch method. the result ind ... | 2016 | 27420072 |
| a report of peritonitis from aeromonas sobria in a peritoneal dialysis (pd) patient with necrotizing fasciitis. | a 70-years of age, male patient with underlying type 2 diabetes mellitus, hypertension, dyslipidemia and ischemic heart disease had undergone continuous ambulatory peritoneal dialysis (capd)for 3 years without any episodes of peritonitis. he was diagnosed with necrotizing fasciitis and later developed peritonitis after receiving a laceration from an aquatic injury suffered during the flood disaster of 2011. the blood culture, necrotic tissue and the clear dialysate collected upon admission had s ... | 2015 | 27276849 |
| aeromonas sobria bacteriemia in an acute lymphoblastic leukemia case in remission. | 2011 | 27263949 | |
| aeromonas sobria infection in farmed mud loach (misgurnus mizolepis) in korea, a bacteriological survey. | a disease outbreak occurred in june 2012 among mud loach cultured on pond farms in jangseong-gun, jeollanam-do, korea. mortality rates reached up to 1.2% in the farm per day. typical clinical signs were bleeding ulcer at the middle portion of head and haemorrhagic erosion of the operculum. based on biochemical characteristics, the causative bacterium isolated from diseased fish was identified as aeromonas sobria. the isolate expressed two haemolytic genes, aerolysin (sob) and haemolysin (asa1) g ... | 2015 | 27175175 |
| design of an immersion vaccine against aeromonad septicemia in perch (perca fluviatilis l.). | the production of an immersion vaccine and the vaccination procedure to immunize fry of perch (perca fluviatilis l.) against pathogenic aeromonas sobria that harbor a type iii secretion system is described. the vaccine, based on chemically inactivated a. sobria, enables rapid vaccination of a large number of fish by immersion of fry in an aqueous vaccine suspension during 5 min, giving them high protection during fattening under open water conditions in a freshwater lake for at least 4 months. | 2016 | 27076300 |
| antibiogram characterization and putative virulence genes in aeromonas species isolated from pig fecal samples. | aeromonas species are broadly distributed in nature and agricultural environments and have been isolated from feces, bedding, and drinking water of healthy pigs. we assessed the incidence, virulence properties, and antimicrobial resistance profile of aeromonas spp., isolated from pig feces. antibiogram was done using the disc diffusion methods, and polymerase chain reaction was used for the detection of putative virulence genes. identification of isolates revealed three phenotypic species with p ... | 2016 | 26971520 |
| effects of dietary acidifiers on growth, hematology, immune response and disease resistance of nile tilapia, oreochromis niloticus. | organic acids or their salts can be used as feed additives in aquaculture. this study was conducted to evaluate the use of a mixture of formic acid, propionic acid and calcium propionate compared with oxytetracycline (otc). a total of 720 apparently healthy oreochromis niloticus fingerlings with an average body weight of 28.8 ± 0.14 g (mean ± se) were randomly divided into four equal groups (g1, g2, g3and g4). g1 was fed a basal diet with no additive as the control group, while g2 and g3 were fe ... | 2016 | 26860238 |
| indolo[3,2-b]quinoline derivatives suppressed the hemolytic activity of beta-pore forming toxins, aerolysin-like hemolysin produced by aeromonas sobria and alpha-hemolysin produced by staphylococcus aureus. | in an attempt to discover inhibitory compounds against pore-forming toxins, some of the major toxins produced by bacteria, we herein examined the effects of four kinds of indolo[3,2-b]quinoline derivatives on hemolysis induced by the aerolysin-like hemolysin (alh) of aeromonas sobria and also by the alpha-hemolysin of staphylococcus aureus. the results showed that hemolysis induced by alh was significantly reduced by every derivative, while that induced by alpha-hemolysis was significantly reduc ... | 2016 | 26725434 |
| [aeromonas sobria infection in patients with acute leukemia:clinical features and outcome]. | to investigate the clinical features and outcome of patients with acute leukemia and aeromonas sobria infection. | 2015 | 26725395 |
| detection and characterization of potentially pathogenic aeromonas sobria isolated from fish hypophthalmichthys molitrix (cypriniformes: cyprinidae). | the current study focuses on the detection and characterization of potentially pathogenic aeromonas sobria from fish silver carp (hypophthalmichthys molitrix). assessment of clinical, microbiological, pathological and biochemical characteristics of a. sobria were taken into account in order to understand the epidemiology, frequency and occurrence of this infection. clinically the infected fish (h. molitrix) was observed for various types of symptoms. a total of 33 colonies of a. sobria strain we ... | 2016 | 26518124 |
| microbiological and parasitological investigation on chelonians reared in italian facilities. | the rapid rise in the number of pet chelonians and their illegal trade can modify the ecology, involving exotic pets, humans, and microbiological agents. therefore, different epidemiological situations and the related risk to introduce and spread infectious diseases, especially zoonotic agents, have to be considered. the aim of this study was to investigate the microbiological and parasitological situation in 2 chelonian facilities (a private breeding of tortoises and a shelter for turtles) coll ... | 2015 | 26344661 |
| molecular cloning and functional characterization of mannose receptor in zebra fish (danio rerio) during infection with aeromonas sobria. | mannose receptor (mr) is a member of pattern-recognition receptors (prrs), which plays a significant role in immunity responses. much work on mr has been done in mammals and birds while little in fish. in this report, a mr gene (designated as zfmr) was cloned from zebra fish (danio rerio), which is an attractive model for the studies of animal diseases. the full-length cdna of zfmr contains 6248 bp encoding a putative protein of 1428 amino acids. the predicted amino acid sequences showed that zf ... | 2015 | 25988382 |
| antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia). | antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ... | 2015 | 25803299 |
| structural basis for action of the external chaperone for a propeptide-deficient serine protease from aeromonas sobria. | subtilisin-like proteases are broadly expressed in organisms ranging from bacteria to mammals. during maturation of these enzymes, n-terminal propeptides function as intramolecular chaperones, assisting the folding of their catalytic domains. however, we have identified an exceptional case, the serine protease from aeromonas sobria (asp), that lacks a propeptide. instead, orf2, a protein encoded just downstream of asp, appears essential for proper asp folding. the mechanism by which orf2 functio ... | 2015 | 25784551 |
| aerobic bacterial microbiota isolated from the cloaca of the european pond turtle (emys orbicularis) in poland. | we conducted a comparative analysis of the aerobic cloacal bacteria of european pond turtles (emys orbicularis) living in their natural environment and juvenile turtles reared under controlled conditions in a breeding center. we included 130 turtles in the study. the aerobic bacteria isolated from the cloaca of the juvenile turtles were less diverse and more prevalent than the bacteria isolated from free-living adults. we isolated 17 bacterial species from juvenile captive turtles, among which t ... | 2015 | 25380369 |
| aeromonas sobria necrotizing fasciitis and sepsis in an immunocompromised patient: a case report and review of the literature. | aeromonas veronii biovar sobria is a rare cause of bacteremia, with several studies indicating that this isolate may be of particular clinical significance since it is enterotoxin producing. a wide spectrum of infections has been associated with aeromonas species in developing countries that include gastroenteritis, wound infections, septicemia and lung infections. this infection, caused by aeromonas species, is usually more severe in immunocompromised than immunocompetent individuals. we here d ... | 2014 | 25245365 |
| antibacterial efficacy of recombinant siganus oramin l-amino acid oxidase expressed in pichia pastoris. | siganus oraminl-amino acid oxidase is a novel natural protein (named sr-laao) isolated from serum of the rabbitfish (s. oramin), which showed antibacterial activity against both gram-positive and gram-negative bacteria and had a lethal effect on the parasites cryptocaryon irritans, trypanosoma brucei brucei and ichthyophthirius multifiliis. in order to test whether recombinant sr-laao (rsr-laao) produced by the eukaryotic expression system also has antimicrobial activity, the yeast pichia pastor ... | 2014 | 25238719 |
| the manx shearwater (puffinus puffinus) as a candidate sentinel of atlantic ocean health. | seabirds have been historically used to monitor environmental contamination. the aim of the present study was to test the suitability of a species belonging to the procellariiformes group, the manx shearwater, puffinus puffinus, as a sentinel of environmental health, by determining contaminant levels (trace metals and organochlorine compounds) from carcass tissues and by isolating vibrio spp. and aeromonas spp. from live specimens. to this end, 35 puffinus puffinus carcasses wrecked on the north ... | 2014 | 25191536 |
| structure of the o-specific polysaccharide from the lipopolysaccharide of aeromonas sobria strain pt312. | the o-specific polysaccharide (ops) obtained by mild-acid degradation of the lipopolysaccharide from aeromonas sobria strain pt312 was studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including 2d 1h,1h cosy, tocsy, noesy, 1h-detected 1h,13c hsqc, and hmbc experiments. the sequence of the sugar residues was determined using 1h,1h noesy and 1h,13c hmbc experiments. it was found that the ops was built up of disaccharide repeating units composed of glcpnac and non-s ... | 2015 | 25037826 |
| cdna cloning and expression characterization of serum transferrin gene from oriental weatherfish misgurnus anguillicaudatus. | in this study, the cdna coding serum transferrin (stf) of misgurnus anguillicaudatus (mastf) was cloned. mastf cdna is composed of 2326 bp with a 2007 bp open reading frame encoding 668 amino acids. the deduced mastf protein consists of a signal peptide, two lobes (n and c-lobes) and signature motifs of transferrin (tf) family. the results of tissue distribution indicated that mastf mrna was predominantly expressed in the liver. the results indicate that the mastf expression increased significan ... | 2014 | 24673686 |
| aeromonas sobria in chlorinated drinking water supplies. | aeromonas species were recovered from over 27% of 183 chlorinated drinking water samples collected during an 18-month period. sixteen of 20 isolates tested elicited a cytotoxic response by y-1 mouse adrenal cells. none of the strains was either enterotoxigenic by the rabbit ligated ileal loop assay, exhibited piliation, or showed significant mannose resistant adherence to human buccal cells. theaeromonas isolates were further identified to bea. sobria and were resistant to ampicillin and suscept ... | 1982 | 24226050 |
| the in-vitro antibacterial effects of organic salts, chemical disinfectants and antibiotics against pathogens of black disease in fairy shrimp of thailand. | the antibacterial effects of organic salts, chemical disinfectants and antibiotics were evaluated on cultures of aeromonas hydrophila c03, aeromonas sobria c26, a. sobria c29, aeromonas caviae c24 and acinetobacter sp. sh-94b, the pathogens that cause black disease found in fairy shrimps (streptocephalus sirindhornae sanoamuang et al. (2000) and branchinella thailandensis sanoamuang, saengphan & murugan) of thailand. the minimal inhibitory concentrations (mics) of organic salts (sodium chloride ... | 2014 | 24131294 |
| molecular cloning and functional characterization of a short peptidoglycan recognition protein (hcpgrps1) from the freshwater mussel, hyriopsis cumingi. | peptidoglycan recognition proteins (pgrps), which are evolutionarily conserved from invertebrates to vertebrates, function as pattern-recognition and effector molecules in innate immunity. in the present study, a short-form pgrp, designated as hcpgrps1 was identified from freshwater mussel hyriopsis cumingi. the deduced amino acid sequence of hcpgrps1 is composed of 235 residues which contains a conserved pgrp domain at the c-terminus. sequence analysis showed that hcpgrps1 shared high identitie ... | 2013 | 23933513 |
| molecular cloning and expression analysis of two β-defensin genes in the blunt snout bream (megalobrama amblycephala). | β-defensins are a group of cysteine-rich, cationic antimicrobial peptides that play important roles in innate immune system against pathogenic microbes invading. in this study, the part-length cdna sequences of two β-defensin genes (maβd-1, maβd-2) in blunt snout bream (megalobrama amblycephala) were identified. homology analysis showed that the cdna sequences of maβd-1 and maβd-2 had high similarities to those in common carp and zebrafish. real-time quantitative pcr results exhibited that expre ... | 2013 | 23876385 |
| infection caused by aeromonas sobria, complicated by lower extremity paraplegia and the cauda equine syndrome in a patient with well-controlled type 2 diabetes. | 2012 | 23671455 | |
| adhesion to brown trout skin mucus, antagonism against cyst adhesion and pathogenicity to rainbow trout of some inhibitory bacteria against saprolegnia parasitica . | biological control of saprolegniosis with bacteria might be an alternative to the use of chemical compounds. among criteria for the selection of such bacteria are their absence of pathogenicity to fish and their ability to prevent adhesion of the pathogen to the skin mucus. the pathogenicity to rainbow trout of 21 bacterial isolates with in vitro inhibitory activity against saprolegnia parasitica was studied. fifteen of the isolates, identified as aeromonas sobria, pantoea agglomerans, pseudomon ... | 2013 | 23670078 |
| skin and soft-tissue infections caused by aeromonas sobria. | 2013 | 23584005 | |
| mortality of therapeutic fish garra rufa caused by aeromonas sobria. | to investigate a case of mass mortality of garra rufa (g. rufa) from a fish hatchery farm in slovakia. | 2012 | 23569873 |
| characterization of the bacterial microbiota of biomphalaria glabrata (say, 1818) (mollusca: gastropoda) from brazil. | roughly 200 000 000 people in 74 countries infected with schistosomes all share the fact that they came in contact freshwater harbouring infected snails. the aim of the study is to characterize the microbiota of wild and laboratory-reared snails of biomphalaria glabrata from pernambuco, brazil. the microbiota of these molluscs was identified biochemically by the vitek 2 automated microbiological system. antimicrobial susceptibility testing was carried out by the disc diffusion method with ß-lact ... | 2013 | 23488866 |
| detection of cyprinid herpesvirus 2 in association with an aeromonas sobria infection of carassius carassius (l.), in italy. | sixteen specimens of female crucian carp, carassius carassius (l.), during the breeding season, were investigated for post-mortem and full diagnostic examination during a mortality outbreak in a tributary stream of the arno river in tuscany in 2011. necropsy highlighted the presence of a swollen anus and widespread haemorrhages in the body, fins, gills and eyes. haemorrhages in internal organs and spleen granulomas were also observed. bacteria isolated from the brain, kidney and spleen of affect ... | 2013 | 23488630 |
| fulminant necrotizing fasciitis caused by aeromonas sobria in neutropenic patients. | infection with aeromonas species has been reported to occur in neutropenic patients. necrotizing fasciitis caused by aeromonas species is uncommon but potentially life-threatening. we herein describe three cases of fulminant necrotizing fasciitis caused by aeromonas sobria in neutropenic patients. these cases shared many clinical characteristics, including shock, coagulopathy, multiple organ failure and rapidly deteriorating and eventually fatal clinical courses. in all cases, aeromonas sobria w ... | 2012 | 23207126 |
| inhibition of aeromonas sobria serine protease (asp) by α2-macroglobulin. | asp is a serine protease secreted by aeromonas sobria. asp cleaves various plasma proteins, which is associated with onset of sepsis complications, such as shock and blood coagulation disorder. to investigate a host defense mechanism against this virulence factor, we examined the plasma for asp inhibitor(s). human plasma inhibited asp activity for azocasein, which was almost completely abolished by treating plasma with methylamine, which inactivates α2-macroglobulin (α2-mg). the asp-inhibitor co ... | 2012 | 23089609 |
| the tail nick augments aeromonas sobria serine protease (asp) activity in plasma through retarding inhibition by α2-macroglobulin. | asp is a serine protease secreted by aeromonas sobria, a sepsis-causing bacterium, and induces sepsis-mimicking disorders through plasma protein cleavage. the pathogen also secretes nasp that has a nick in the carboxy-terminal region. compared with single-chain asp (sasp), nasp had near-equivalent activity for small peptide substrates but was less proteolytic. surprisingly, nasp cleaved proteins more in plasma and was inhibited by human α(2)-macroglobulin more slowly than sasp. retarded inhibiti ... | 2012 | 22971340 |
| molecular cloning, recombinant expression and antibacterial activity analysis of hepcidin from simensis crocodile (crocodylus siamensis). | hepcidin, a cysteine-rich cationic antibacterial peptide, plays an important role in human defense against pathogen infection. however, its role in reptile immune response and whether it is involved in antibacterial immune have not yet been proven. in order to study the antibacterial activity of crocodylus siamensis hepcidin (cshepc), a common reptile which lives in topic region of southeast asia, a cdna sequence of cshepc was cloned, which included an open reading frame (orf) of 300 bp encoding ... | 2013 | 22967859 |
| first description of cole-type plasmid in aeromonas spp. carrying quinolone resistance (qnrs2) gene. | isolation and full sequence analysis of cole-type plasmid, which carries the qnrs2 gene. | 2012 | 22862417 |
| molecular detection of the aeromonas virulence aerolysin gene in retail meats from different animal sources in egypt. | meat commonly contain the same aeromonas spp. which occur in human diarrhoeal and non-diarrhoeal faecal samples. motile aeromonas were isolated from 5.6% of total 302 samples. the distribution of the isolates were 5.9 and 5.2% in fresh and frozen samples, respectively. of the 302 samples taken of the four animal meat species investigated, the genus aeromonas were isolated in 12.3% of the fresh samples collected from buffalo meat, in 6.5% of the samples collected from sheep meat and 14.0% from th ... | 2012 | 22806009 |
| recombinant aeromonas hydrophila outer membrane protein 48 (omp48) induces a protective immune response against aeromonas hydrophila and edwardsiella tarda. | the gene coding for an outer membrane protein omp48 of aeromonas hydrophila isolated from an infected fish was cloned and sequenced. analysis of nucleotide sequence showed the omp48 gene to be an adhesin encoding a protein of 426 amino acids with high identity to the omp48 gene of aeromonas veronii, another fish pathogen. the gene belonged to the maltoporin group of porins and had high similarity to lamb porins of a. hydrophila, aeromonas salmonicida and vibrio parahaemolyticus. the expressed pu ... | 2012 | 22564558 |
| culturable gut microbiota diversity in zebrafish. | the zebrafish (danio rerio) is an increasingly used laboratory animal model in basic biology and biomedicine, novel drug development, and toxicology. the wide use has increased the demand for optimized husbandry protocols to ensure animal health care and welfare. the knowledge about the correlation between culturable zebrafish intestinal microbiota and health in relation to environmental factors and management procedures is very limited. a semi-quantitative level of growth of individual types of ... | 2012 | 22428747 |
| production and properties of lipase of aeromonas sobria. | aeromonas have been isolated from a wide variety of aquatic environments. however the number of aeromonas in sea water is extremely small compared to that in fresh water. in in vitro culture, aeromonas can grow in mediums containing nacl at a concentration of 3.0%, this concentration corresponding to that of sea water. it is unclear why the number of aeromonas is low in sea water. exoproteins of bacteria are thought to be important for bacterial growth and survival in the environment. previously ... | 2012 | 22376235 |
| bacterial sepsis following prostatic biopsy. | despite the recent innovations, complications of prostate biopsy can occur. the aim of this study was a prospective monitoring of major septic complications occurring after transrectal prostate biopsy, to describe their causing agents, to report the clinical course of these patients, and to give guidelines based on our personal experience. | 2012 | 22371127 |
| Structural and Functional Analysis of the Serine Protease from Aeromonas sobria. | Aeromonas species are Gram-negative facultative anaerobic bacteria found ubiquitously in a variety of aquatic environments and most commonly implicated as causative agents of gastroenteritis. Sepsis is a fatal complication of Aeromonas infectious diseases, particularly in immune-compromised patients. Two species, Aeromonas hydrophila and Aeromonas sobria, are associated with human disease. Feasible virulence factors of Aeromonas include fimbrial and afimbrial adhesion molecules, hemolysins, ente ... | 2011 | 22129876 |
| [fatal aeromonas sobria infection in liver cirrhosis]. | aeromonas sobria infection is known to be very serious in immunocompromised hosts. we report a case of a. sobria infection fatal in a subject with decompensated liver cirrhosis. a 63-year-old man with liver cirrhosis admitted for fever and vomiting developed a necrotizing soft-tissue infection in the right lower leg. despite a decompression incision in the affected limb and intensive care, he died of sepsis one day after surgery. a. sobria was detected afterward in a blood culture. | 2011 | 22117380 |
| [preparation and identification of ompw monoclonal antibodies]. | to prepare and characterize the mouse monoclonal antibodies against vibrio parahaemolyticus ompw. | 2011 | 21906472 |
| three goose-type lysozymes in the gastropod oncomelania hupensis: cdna sequences and lytic activity of recombinant proteins. | three goose-type (g-type) lysozymes, designated as ohlysg1, ohlysg2 and ohlysg3 were identified from expressed sequence tags (ests) of a gastropod oncomelania hupensis, the intermediate host of schistosoma japonicum. the full cdna sequences of ohlysg1, ohlysg2 and ohlysg3 consisted of 735, 909 and 808 nucleotides, with an open reading frame of 198, 214 and 249 codons containing a 21, 7 and 8 amino acid (aa) signal peptide at the n-terminus, respectively. the three g-type lysozymes shared conserv ... | 2011 | 21756934 |
| the influence of environmental bacteria in freshwater stingray wound-healing. | invasion by bacteria can influence the course of healing of wounds acquired in aquatic environment. in this study, the bacteria present in potamotrygonmotoro stingray mucus and in the alto paraná river water were identified, and their ability to induce tissue injury and resist antibiotics was determined. biochemical identification analysis showed that 97% of all bacterial isolates were gram negative, aeromonas spp., enterobacter cloacae and citrobacter freundii being the species most prevalent. ... | 2011 | 21635911 |
| inhibition of biosynthesis of metalloprotease of aeromonas sobria by sodium chloride in the medium. | the present authors have previously shown that the serine protease activity of aeromonas sobria is markedly decreased when a. sobria is cultured in medium containing 3.0% sodium chloride (nacl, concentration almost equivalent to sea water salinity), and that this occurs because, although the synthesis of asp is not disturbed by the salt in the medium, the maturation pathway of serine protease of a. sobria (asp) does not proceed successfully in such a medium. in this study, the effect of salt in ... | 2011 | 21175775 |
| enhancement of protective immunity in european eel (anguilla anguilla) against aeromonas hydrophila and aeromonas sobria by a recombinant aeromonas outer membrane protein. | to develop a vaccine, which can simultaneously prevent the diseases caused by various pathogenic bacteria in fish, we try to find a conserved outer membrane protein (omp) antigen from different bacterial pathogens. in this study, an omp fragment of 747 bp (named as omp-g), which was highly conserved in seven aeromonas omp sequences from the ncbi database, was amplified by pcr from one aeromonas sobria strain (b10) and two aeromonas hydrophila strains (b27 and b33) with the designed specific prim ... | 2010 | 21148192 |
| maturation pathway of metalloprotease produced by aeromonas sobria. | previously, we cloned the metalloprotease gene of aeromonas sobria (amp) and determined its nucleotide sequence (genbank accession number dq784565). the protease is composed of 591 amino acid residues. in this study, we purified the mature metalloprotease from the culture supernatant of a. sobria and determined the amino terminal sequence and molecular size of amp. in addition, we examined the production of amp diachronically and found that amp emerges outside of the cell as an intermediate comp ... | 2010 | 21118297 |
| simple and direct detection of aeromonas hydrophila infection in the goldfish, carassius auratus (l.), by dot blotting using specific monoclonal antibodies. | a combination of eight isolates of aeromonas hydrophila was used to produce monoclonal antibodies (mabs). ten different groups of mabs specific to aeromonas were selected. the first five groups of mabs demonstrated high specificity and bound to only one or two isolates of a. hydrophila. the sixth and the seventh groups of mabs were a. hydrophila specific. they recognized seven of eight a. hydrophila isolates (ah1, 2, 3, 4, 5, 6, 8); however, the mab in the seventh group also showed cross-reactiv ... | 2010 | 21091724 |
| identification and pathogenicity of aeromonas sobria on tail-rot disease in juvenile tilapia oreochromis niloticus. | thirty-six strains, numbered from py01 to py36, were isolated from six moribund oreochromis niloticus. the biochemical characteristics of all strains conformed to the species description of aeromonas sobria on the basis of api 20e and biolog gn system. furthermore, gyrb sequence of strain py36 was sequenced and showed high similarity (99.8%) with a. sobria in genbank. antibiotic-resistance of strain py36 was assessed by the kirby-bauer disk diffusion method, and the results showed it was suscept ... | 2011 | 20853167 |
| quorum sensing in aeromonas species isolated from patients in malaysia. | bacterial quorum sensing signal molecules called n-acylhomoserine lactone (ahl) controls the expression of virulence determinants in many gram-negative bacteria. we determined ahl production in 22 aeromonas strains isolated from various infected sites from patients (bile, blood, peritoneal fluid, pus, stool and urine). all isolates produced the two principal ahls, n-butanoylhomoserine lactone (c4-hsl) and n-hexanoyl homoserine lactone (c6-hsl). ten isolates also produced additional ahls. this re ... | 2011 | 20544198 |
| clinical presentations, prognostic factors, and mortality in patients with aeromonas sobria complex bacteremia in a teaching hospital: a 5-year experience. | bacteremia due to aeromonas sobria complex is an uncommon clinical presentation, associated with a high mortality rate. this retrospective study reviewed the clinical manifestations and prognostic factors of a. sobria complex bacteremia. | 2009 | 20422137 |
| association between environmental microbiota and indigenous bacteria found in hemolymph, extrapallial fluid and mucus of anodonta cygnea (linnaeus, 1758). | bivalves filter and accumulate large numbers of microorganisms present in the harvesting water. a complete understanding of the balance between anodonta cygnea and the microbiota present in their surrounding environment remains incomplete. therefore, the aim of this study was to quantify and identify the indigenous bacteria in the biological fluids of a. cygnea collected from mira lagoon in northern portugal. the results showed vibrio metschnikovii and aeromonas sobria as the dominant groups. th ... | 2010 | 20349058 |
| aeromonas rivuli sp. nov., isolated from the upstream region of a karst water rivulet. | two freshwater isolates (wb4.1-19(t) and wb4.4-101), sharing 99.9 % 16s rrna gene sequence similarity, were highly related to aeromonas sobria (99.7 % similarity; 6 bp differences). a phylogenetic tree derived from a multi-locus phylogenetic analysis (mlpa) of the concatenated sequences of five housekeeping genes (gyrb, rpod, reca, dnaj and gyra; 3684 bp) revealed that both strains clustered as an independent phylogenetic line next to members of aeromonas molluscorum and aeromonas bivalvium. the ... | 2011 | 20207806 |
| rosmarinus officinalis l.: chemical modifications of the essential oil and evaluation of antioxidant and antimicrobial activity. | rosmarinus officinalis essential oil was separated into its hydrocarbon and oxygenated fractions. the major compounds in the hydrocarbon fraction were alpha-pinene (44.2%), camphene (24.5%), and limonene (11.7%), while in the oxygenated fraction they were 1,8-cineole (37.6%), camphor (16.5%), and bornyl acetate (21.4%). the hydrocarbon fraction was submitted to a hydroformylation process and the antioxidant activity of the product was screened by the dpph and beta-carotene/linoleic acid tests. t ... | 2009 | 20120107 |
| the carboxy-terminal tail of aeromonas sobria serine protease is associated with the chaperone. | asp is the only bacterial protease in the kexin group of the subtilisin family. previous studies have revealed that the orf2 protein encoded at the 3' end of the asp operon is required for asp to change from a nascent form into an active form in the periplasm. however, the mechanism by which orf2 makes contact and interacts with asp in the maturation process remains unknown. the present study examined the effect of mutations in the carboxy-terminal region of asp on the asp maturation process. bo ... | 2009 | 19954452 |
| cellular components of probiotics control yersinia ruckeri infection in rainbow trout, oncorhynchus mykiss (walbaum). | subcellular components of the probiotics aeromonas sobria gc2 and bacillus subtilis jb-1, when administered to rainbow trout, oncorhynchus mykiss, conferred protection against a new biogroup of yersinia ruckeri. thus, intraperitoneal or intramuscular injection of rainbow trout with cell wall proteins (cwps), outer membrane proteins (omps), lipopolysaccharides (lps), whole cell proteins (wcps) and live cells followed by challenge on day 8 with y. ruckeri led to 80-100% survival compared with 10% ... | 2010 | 19912460 |
| a novel method for the diagnosis of drowning by detection of aeromonas sobria with pcr method. | the acid digestion method has been widely used for the diagnosis of death by drowning, but it is not always sensitive. however, there has been no definitive method to replace acid digestion until now. we speculate that bacteria are more useful markers than plankton for the diagnosis of death by drowning. in this study, from the preserved blood samples of 32 freshwater drowning cases, specific dna fragments of aeromonas sobria, one of the most common aquatic bacteria, were examined using pcr. the ... | 2009 | 19766051 |
| structural basis for the kexin-like serine protease from aeromonas sobria as sepsis-causing factor. | the anaerobic bacterium aeromonas sobria is known to cause potentially lethal septic shock. we recently proposed that a. sobria serine protease (asp) is a sepsis-related factor that induces vascular leakage, reductions in blood pressure via kinin release, and clotting via activation of prothrombin. asp preferentially cleaves peptide bonds that follow dibasic amino acid residues, as do kex2 (saccharomyces cerevisiae serine protease) and furin, which are representative kexin family proteases. here ... | 2009 | 19654332 |
| aeromonas fluvialis sp. nov., isolated from a spanish river. | a gram-stain-negative, facultatively anaerobic bacterial strain, designated 717(t), was isolated from a water sample collected from the muga river, girona, north-east spain. preliminary analysis of the 16s rrna gene sequence showed that this strain belonged to the genus aeromonas, the nearest species being aeromonas veronii (99.5 % similarity, with seven different nucleotides). a polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (gyrb, rpod, reca, dnaj and g ... | 2010 | 19648345 |
| pseudomonas chlororaphis strain jf3835 reduces mortality of juvenile perch, perca fluviatilis l., caused by aeromonas sobria. | motile aeromonad septicaemia caused by aeromonas sobria is a cause of disease in farmed perch, perca fluviatilis l., in switzerland. we have evaluated the potential of a pseudomonas chlororaphis isolate, obtained from perch intestine, to control a. sobria infection. inoculation of juvenile perch with p. chlororaphis strain jf3835 prior to infection with a. sobria caused a reduction in a. sobria associated mortalities. infection of perch with xyle-labelled p. chlororaphis indicated the bacterium ... | 2009 | 19476558 |
| appendicitis associated with travelers' diarrhea caused by aeromonas sobria. | aeromonas species is an infrequent pathogen causing travelers' diarrhea and gastroenteritis. no human case of appendicitis has been reported as a complication of aeromonas infection until now. we describe a case of acute appendicitis associated with aeromonas sobria infection acquired in cebu, philippines. | 2009 | 19335814 |
| toxin production by aeromonas sobria in natural environments: river water vs. seawater. | aeromonas are water-borne pathogens. they are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% nacl). however, the presence of aeromonas in seawater is extremely rare compared with that in river water. in this study, we tested the ability of aeromonas sobria to produce toxins in river water and seawater. first, we cultured a. sobria on skim milk agar plates supplemented with either river water (sarw) or seawater (sasw). the ... | 2008 | 19122681 |
| aeromonas sobria sepsis complicated by rhabdomyolysis in an hiv-positive patient: case report and evaluation of traits associated with bacterial virulence. | human infection with aeromonas species is uncommon and most often due to trauma with exposure to contaminated water or soil. a 43-year-old hiv- and hepatitis c virus (hcv)-infected male, after a two-week course of corticosteroid therapy for an autoimmune anemia, developed diarrhea, dermatologic manifestations and a multiple organ dysfunction syndrome, resulting in death. although stool samples were repeatedly negative, two sets of blood cultures obtained during a single peak of fever yielded the ... | 2009 | 18977680 |
| case reports: fatal necrotizing fasciitis caused by aeromonas sobria in two diabetic patients. | we report two rare cases of aeromonas sobria necrotizing fasciitis with sepsis in patients with diabetes. in both cases, immediate fasciotomy was performed and appropriate empiric antimicrobial therapy and intensive care were administered. however, the two patients died on day 2 and day 11, respectively, after admission as a result of multiple organ failure. when patients present with a rapid onset of skin necrosis and progressive sepsis, an aeromonas sobria infection or vibrio infection should ... | 2009 | 18800212 |
| production of c5a by asp, a serine protease released from aeromonas sobria. | aeromonas sobria causes pus and edema at sites of infection. however, the mechanisms underlying these effects have not been elucidated. c5a, the amino-terminal fragment of the complement 5th component (c5), mimics these events. to investigate the involvement of c5a in the pathophysiology of a. sobria infection, we examined release of c5a from human c5 by a serine protease (asp), a putative virulence factor secreted by this bacterium. c5 incubated with enzymatically active asp induced neutrophil ... | 2008 | 18714034 |
| [characterization of aeromonas spp isolates from newborns hospitalized]. | aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ... | 2008 | 18545840 |
| impaired plasma clottability induction through fibrinogen degradation by asp, a serine protease released from aeromonas sobria. | aeromonas sobria infection often advances to sepsis, in which interaction of bacterial components with plasma proteins possibly causes various disorders. this bacterium releases a serine protease (asp), a putative virulence factor, and binds to fibrinogen. to study the asp effect on fibrinogen, we incubated fibrinogen or plasma with asp and investigated their clotting elicited by thrombin, which converts fibrinogen to a fibrin clot. enzymatically active asp retarded plasma clotting in a dose-dep ... | 2008 | 18462393 |
| efficacy of in-feed probiotics against aeromonas bestiarum and ichthyophthirius multifiliis skin infections in rainbow trout (oncorhynchus mykiss, walbaum). | the aim of this study was to assess the efficacy of in-feed probiotics as a preventive measure against skin infections caused by aeromonas bestiarum and ichthyophthirius multifiliis (ich) in rainbow trout. | 2008 | 18422955 |
| identification of bacteria from the normal flora of perch, perca fluviatilis l., and evaluation of their inhibitory potential towards aeromonas species. | pathogenic aeromonas sobria has been identified as a causative agent of ulcerative disease in farmed european perch, perca fluviatilis l. to study the effect of the normal intestinal bacterial flora of perch against a. sobria, we sampled 193 bacterial isolates from the perch digestive tract. the isolates were identified by sequence analysis of the 16s rrna gene and their inhibitory potential against a. sobria was evaluated in vitro. nineteen of the strains isolated showed inhibition and were als ... | 2008 | 18355180 |
| fluid secretion caused by aerolysin-like hemolysin of aeromonas sobria in the intestines is due to stimulation of production of prostaglandin e2 via cyclooxygenase 2 by intestinal cells. | to clarify the mechanisms of diarrheal disease induced by aeromonas sobria, we examined whether prostaglandin e2 (pge2) was involved in the intestinal secretory action of a. sobria hemolysin by use of a mouse intestinal loop model. the amount of pge2 in jejunal fluid and the fluid accumulation ratio were directly related to the dose of hemolysin. the increase over time in the level of pge2 was similar to that of the accumulated fluid. in addition, hemolysin-induced fluid secretion and pge2 synth ... | 2008 | 18086811 |
| activation of prothrombin by asp, a serine protease released from aeromonas sobria. | the effect of a serine protease (asp) secreted from aeromonas sobria on plasma coagulation was investigated. proteolytically active asp promoted human plasma coagulation in a dose-dependent manner. consistent with the preference for a factor xa-specific oligo-peptide substrate, asp produced enzymatic activity from human prothrombin but not from factors ix and x. asp cleaved prothrombin to produce enzymatically active 37 kda-fragment displaying the same molecular mass as alpha-thrombin. asp is th ... | 2007 | 18067862 |
| aeromonas sobria prostatitis and septic shock in a healthy man with chronic alcoholic consumption. | prostate infection caused by aeromonas sobria has not been reported in the literature. we presented a 44-year-old healthy man with a habit of alcoholic consumption who was admitted because of primary a. sobria prostatitis with septicemia. the patient was successfully treated with intravenous ofloxacin and was uneventful after a half-year follow-up. based on our rare case, we suggest to clinical physicians that prostatitis might be considered in healthy male patients with a. sobria bacteremia and ... | 2007 | 18032845 |
| proteomic analysis of rainbow trout (oncorhynchus mykiss, walbaum) serum after administration of probiotics in diets. | the response of rainbow trout (oncorhynchus mykiss, walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (apr). proteomic analysis by two-dimensional electrophoresis (2d) concurrently with mass spectrometry was used to detect apr related proteins in rainbow trout serum following feeding with probiotics aeromonas sobria gc2 and bacillus sp. jb-1. three candidate proteins increased following use of gc2, and wer ... | 2008 | 17981340 |
| salt in surroundings influences the production of serine protease into milieu by aeromonas sobria. | previously we have shown that the open reading frame 2 protein (orf2 protein), which is encoded at the 3 ' end of serine protease of aeromonas sobria (asp), functions as a chaperone protein in periplasm in the production of asp. both proteins, asp and orf2 protein, associate in periplasm and orf2 protein helps asp to take an active form. asp which is dissociated from orf2 protein emerges in milieu . in this study, we examined the effect of sodium chloride (nacl) in medium on asp production by a. ... | 2007 | 17951986 |
| molecular characterization of goose-type lysozyme homologue of large yellow croaker and its involvement in immune response induced by trivalent bacterial vaccine as an acute-phase protein. | lysozyme acts as an innate immunity molecule against the invasion of bacterial pathogens. here, the cdna of a goose-type lysozyme (g-lysozyme) was cloned from large yellow croaker (pseudosciana crocea) by expressed sequence tags (est) and race-pcr techniques. the full-length cdna of large yellow croaker g-lysozyme (lycgl) is 716 nucleotides (nt) encoding a protein of 193 amino acids (aa), with a theoretical molecular weight of 21.3kda. the deduced lycgl possessed the typical structural features ... | 2007 | 17850883 |
| the development of probiotics for the control of multiple bacterial diseases of rainbow trout, oncorhynchus mykiss (walbaum). | jb-1 and gc2, which were equated with bacillus sp. and aeromonas sobria respectively, were recovered from the digestive tract of rainbow trout, oncorhynchus mykiss and ghost carp, cyprinus sp. respectively, and demonstrated effectiveness as probiotics for the control of infections caused by aeromonas salmonicida, lactococcus garvieae, streptococcus iniae, vibrio anguillarum, vibrio ordalii and yersinia ruckeri. when administered to rainbow trout (average weight = 12 g) for 14 days in feed dosed ... | 2007 | 17850573 |
| rapid detection of virulence factors of aeromonas isolated from a trout farm by hexaplex-pcr. | the detection of virulence factors of aeromonas is a key component in determining potential pathogenicity because these factors act multifunctionally and multifactorially. in this study water samples were collected from a trout farm on a seasonal basis, and diseased fish and aeromonas species were isolated and identified. for rapid detection of six virulence factors of isolated aeromonas, a hexaplex-polymerase chain reaction (hexaplex-pcr) assay was used. the detected virulence factors include a ... | 2007 | 17846582 |
| the immune response and protective efficacy of vaccination with oral microparticle aeromonas sobria vaccine in mice. | the effect of an oral alginate microparticle aeromonas sobria vaccine on immune response and protection of mice was investigated. the formalin-killed cultures (fkc) of a. sobria strain z-1 treated with three methods were incorporated into alginate microparticles, and the encapsulated vaccines were orally used for immunizing mice. four weeks after immunization, the serum agglutinating antibody titers, bactericidal activity of leucocytes and relative percent survival (rps) of mice immunized orally ... | 2007 | 17630205 |
| the immune response and protective efficacy of oral alginate microparticle aeromonas sobria vaccine in soft-shelled turtles (trionyx sinensis). | aeromonas sobria is an important bacterial pathogen of soft-shelled turtles (trionyx sinensis). the effect of an oral alginate microparticle a. sobria vaccine (mav) on immune response and protection efficacy of soft-shelled turtles was investigated. the formalin-killed cultures of a. sobria strain z-1 (fkc) were incorporated into alginate microparticles, and the encapsulated vaccine was orally used for immunizing turtles. the serum agglutinating antibody titers, bactericidal activity of blood le ... | 2007 | 17628697 |