Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| isolation and identification of nonoral pathogenic bacteria in the oral cavity of patients with removable dentures. | dentures in the oral cavity may act as a reservoir of microorganisms, which may be related to systemic infections. the aim of this study was to investigate the nonoral pathogenic bacteria in the oral cavity of patients with removable dentures in shiraz, southern iran. | 2017 | 28852636 |
| high mortality rate associated with kpc-producing enterobacter cloacae in a brazilian hospital. | we describe a clonal dissemination of kpc-producing enterobacter cloacae in a brazilian hospital. patients diagnosed with theses isolates showed high mortality rate (41.8%) and were associated with previous use of antibiotics and urinary catheterization. therefore, infection control measures and use of stricter antibiotic policies are required to control the spread of these organisms. | 2017 | 28844375 |
| persistent gram-negative neurosurgical meningitis in a neonate, successfully treated with intraventricular colistin: case report and review of the literature. | we present a case of gram-negative neurosurgical meningitis in a neonate, refractory to optimal intravenous therapy and removal of ventriculo-peritoneal shunt. cerebrospinal fluid was sterilized within 24 hours using intraventricular colistin. this is the first report of intraventricular colistin use in neonatal meningitis caused by enterobacter cloacae. | 2017 | 28841583 |
| transmission and characterization of bla ndm-1 in enterobacter cloacae at a teaching hospital in yunnan, china. | in recent years, new delhi metallo-beta-lactamases 1 (bla ndm-1) has been reported with increasing frequency and become prevalent. the present study was undertaken to investigate the epidemiological dissemination of the bla ndm-1 gene in enterobacter cloacae isolates at a teaching hospital in yunnan, china. | 2017 | 28830556 |
| antimicrobial activity of ceftazidime-avibactam tested against multidrug-resistant enterobacteriaceae and pseudomonas aeruginosa isolates from united states medical centers (2013--2016). | the in vitro activity of ceftazidime-avibactam and many comparator agents was determined against various resistant subsets of organisms selected among 36,380 enterobacteriaceae and 7,868 pseudomonas aeruginosa. isolates were consecutively collected from 94 us hospitals, and all isolates were tested for susceptibility by reference broth microdilution methods in a central monitoring laboratory (jmi laboratories). enterobacteriaceae isolates resistant to carbapenems (cre) and/or ceftazidime-avibact ... | 2017 | 28827415 |
| draft genome sequence of enterobacter cloacae st520 harbouring blakpc-2, blactx-m-15 and blaoxa-17 isolated from coastal waters of the south atlantic ocean. | aquatic environments have contributed to the dissemination of multidrug-resistant (mdr) bacteria, representing a risk for humans and animals. the aim of this study was to report the first draft genome sequence of a mdr enterobacter cloacae strain recovered from seawater in a public beach in brazil. | 2017 | 28827199 |
| a simple phenotypic method for screening of mcr-1-mediated colistin resistance. | to evaluate a novel method, named colistin-mac test, for phenotypic screening of acquired colistin-resistance mediated by transferable mcr-1 resistance determinants, based on colistin mic reduction in the presence of dipicolinic acid (dpa). | 2017 | 28827120 |
| application of maldi-tof ms fingerprinting as a quick tool for identification and clustering of foodborne pathogens isolated from food products. | foodborne pathogens can be associated with a wide variety of food products and it is very important to identify them to supply safe food and prevent foodborne infections. since traditional techniques are time-consuming and laborious, this study was designed for rapid identification and clustering of foodborne pathogens isolated from various restaurants in al-qassim region, kingdom of saudi arabia (ksa) using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ... | 2017 | 28825446 |
| antimicrobial activity of octenidine against multidrug-resistant gram-negative pathogens. | multidrug-resistant (mr) gram-negative (gn) pathogens pose a major and growing threat for healthcare systems, as therapy of infections is often limited due to the lack of available systemic antibiotics. well-tolerated antiseptics, such as octenidine dihydrochloride (oct), may be a very useful tool in infection control to reduce the dissemination of mrgn. this study aimed to investigate the bactericidal activity of oct against international epidemic clones of mrgn. a set of five different species ... | 2017 | 28825186 |
| complete genome sequence of enterobacter cloacae 704sk10, an oxa-48-encoding wastewater isolate. | here we present the complete genome sequence of enterobacter cloacae 704sk10, a swiss wastewater isolate encoding an oxa-48 carbapenemase. assembly resulted in closed sequences of the 4,876,946-bp chromosome, a 111,184-bp incf plasmid, and an oxa-48-encoding incl plasmid (63,458 bp) nearly identical to the previously described plasmid poxa-48. | 2017 | 28818912 |
| draft genome sequences of two enterobacter cloacae subsp. cloacae strains isolated from australian hematology patients with bacteremia. | enterobacter cloacae is a common member of the gut microbiota in healthy individuals. however, it is also an opportunistic pathogen, capable of causing bacteremia. we report the draft genomes of two enterobacter cloacae subspecies cloacae strains isolated from hematology patients with bacteremia. both isolates carry genes encoding extended-spectrum β-lactamases. | 2017 | 28818892 |
| rapid phenotypic stress-based microfluidic antibiotic susceptibility testing of gram-negative clinical isolates. | bacteremia is a life-threatening condition for which antibiotics must be prescribed within hours of clinical diagnosis. since the current gold standard for bacteremia diagnosis is based on conventional methods developed in the mid-1800s-growth on agar or in broth-identification and susceptibility profiling for both gram-positive and gram-negative bacterial species requires at least 48-72 h. recent advancements in accelerated phenotypic antibiotic susceptibility testing have centered on the micro ... | 2017 | 28808348 |
| high prevalence of extended-spectrum β-lactamase-producing enterobacteriaceae in wild fish from the mediterranean sea in algeria. | we investigated the prevalence of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae among wild fish from the coast of bejaia (algeria) in the mediterranean sea. | 2017 | 28805537 |
| the first enterobacter cloacae co-producing ndm and oxa-48 carbapenemases and interhospital spread of oxa-48 and ndm-producing klebsiella pneumoniae in turkey. | the aim of this study was to investigate the occurrence of carbapenemase-producing enterobacteriaceae. | 2017 | 28792697 |
| the occurrence of ctx-m-25-producing enterobacteriaceae in day-old broiler chicks in japan. | day-old chicks from 3 hatcheries were placed on bedding paper and brought to a commercial broiler farm between january and july 2016. sixty-six samples of the paper, which were stained with meconium droppings of the chicks, were collected and examined for isolation of cephalosporin-resistant enterobacteriaceae. cefotaxime (ctx)-resistant klebsiella pneumoniae (1 isolate) and enterobacter cloacae (4 isolates) were isolated from 5 (7.58%) of the 66 samples. conjugation experiments revealed that th ... | 2017 | 28781343 |
| treatment of gaseous volatile organic compounds using a rotating biological filter. | rotating biological filter (rbf), which provides higher oxygen mass transfer has been developed for treating gaseous volatile organic compounds (vocs) such as btex (benzene, toluene, ethylbenzene and xylene) at higher concentrations. the screening of enriched cultures has been done initially to enhance the performance of rbf for treating xylene, toluene and xylene, and btex at various loading rates. the removal efficiency of btex was maximum (82%), higher than toluene and xylene (79%), and xylen ... | 2017 | 28780260 |
| antimicrobial resistance of 3 types of gram-negative bacteria isolated from hospital surfaces and the hands of health care workers. | there has been an increased focus in recent years on antimicrobial resistance of bacteria isolated from clinical samples. however, resistance of bacteria from hospital environments has been less frequently investigated. | 2017 | 28780198 |
| photoinactivation of multidrug resistant bacteria by monomeric methylene blue conjugated gold nanoparticles. | multidrug resistant (mdr) bacterial infections have become a severe threat to the community health due to a progressive rise in antibiotic resistance. nanoparticle-based photodynamic therapy (pdt) is increasingly been adopted as a potential antimicrobial option, yet the cytotoxicity associated with pdt is quite unspecific. herein, we show concanavalin-a (cona) directed dextran capped gold nanoparticles (gnpdex-cona) enhanced the efficacy and selectivity of methylene blue (mb) induced killing of ... | 2017 | 28778019 |
| recurrent fuo due to intermittent enterobacter cloacae bacteremias from an infected pacemaker lead diagnosed by gallium scan. | fever of unknown origin (fuo) refers to fevers of ≥101° f that persist for ≥3 weeks and remain undiagnosed after a focused inpatient or outpatient workup. fuo may be due to infectious, malignant/neoplastic, rheumatic/inflammatory, or miscellaneous disorders. recurrent fuos are due to the same causes of classical fuos. recurrent fuos may have continuous or intermittent fevers and are particularly difficult to diagnose. with intermittent fever, recurrent fuo diagnostic tests are best obtained duri ... | 2017 | 28768449 |
| [analysis of drug resistance of acinetobacter baumannii in wound of children with traffic injury and its relationship with antibiotic use]. | objective: to know the drug resistance of acinetobacter baumannii (ab) in wound of children with traffic injury and its relationship with antibiotic use. methods: wound exudate of 226 children with traffic injury admitted to our unit from january 2010 to december 2015 were collected. api bacteria identification panels and fully automatic microbiological identification system were used to identify pathogens. kirby-bauer paper disk diffusion method was used to detect the drug resistance of pathoge ... | 2017 | 28763905 |
| a prospective randomised trial of isolated pathogens of surgical site infections (ssi). | every surgical wound is colonized by bacteria, but only a small percentage displays symptoms of infection. the distribution of pathogens isolated in surgical site infections has not significantly changed over the last decades. staph. aureus, coag(-) staphylococci, enterococcus spp and e. coli are the main strains appearing. in addition, a continuously rising proportion of surgical site infections caused by resistant bacterial species (mrsa, c. albicans) has been reported. | 2017 | 28761643 |
| draft genome sequence of an aminoglycoside-resistant rmtd-2-producing enterobacter cloacae subsp. cloacae (st)395 in brazil. | enterobacter cloacae has recently emerged as an important agent of nosocomial infections, due to the diffusion of extended spectrum β-lactamases and carbapenemases in this species. in this context, a raise in the use of aminoglycosides for therapy was noticed, being followed by the accelerated development of resistance mechanisms. in this study, we report the draft genome sequence of a multidrug-resistant enterobacter cloacae subsp. cloacae strain (ec2), isolated from active surveillance culture ... | 2017 | 28757348 |
| identification of an operon involved in fluoride resistance in enterobacter cloacae frm. | fluorine is ubiquitous and the most active non-metal element in nature. while many microorganisms have developed fluoride resistance as a result of the widespread and prolonged application of oral hygiene products, the mechanisms used by these organisms to overcome fluoride toxicity are incompletely understood. in this study, a fluoride-resistant strain, enterobacter cloacae frm, was identified which could grow well at a fluoride concentration of 4,000 mg/l. according to comparative genomics, tr ... | 2017 | 28754999 |
| antimicrobial effects of lysophosphatidylcholine on methicillin-resistant staphylococcus aureus. | methicillin-resistant staphylococcus aureus (mrsa) is an important health care-associated and community-associated pathogen and causes a large number of infections worldwide. for the purpose of application to topical treatment of mrsa infection, we examined the antimicrobial effects of lysophosphatidylcholine (lpc) on mrsa strains. we also investigated the combination effect of lpc and gentamicin on mrsa growth. | 2017 | 28748087 |
| evaluation of the rapid polymyxin np test for polymyxin b resistance detection using enterobacter cloacae and enterobacter aerogenes isolates. | background: polymyxin resistance is an increasing problem worldwide. currently, determining susceptibility to polymyxins is problematic and lengthy. polymyxins diffuse poorly into agar, potentially giving inaccurate disk diffusion and etest results. a rapid screening test (2 h) for detection of polymyxin-resistance in enterobacteriaceae, developed by nordmann and poirel (rapid polymyxin np test, 2016), detects glucose metabolization in the presence of polymyxins e and b via ph-induced color chan ... | 2017 | 28747375 |
| in-vivo electrochemical monitoring of h2o2 production induced by root-inoculated endophytic bacteria in agave tequilana leaves. | a dual-function platinum disc microelectrode sensor was used for in-situ monitoring of h2o2 produced in a. tequilana leaves after inoculation of their endophytic bacteria (enterobacter cloacae). voltammetric experiments were carried out from 0.0 to -1.0v, a potential range where h2o2 is electrochemically reduced. a needle was used to create a small cavity in the upper epidermis of a. tequilana leaves, where the fabricated electrochemical sensor was inserted by using a manual three-dimensional mi ... | 2017 | 28746900 |
| multidrug resistant bacteria are sensitive to euphorbia prostrata and six others cameroonian medicinal plants extracts. | multidrug resistant (mdr) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them. | 2017 | 28743314 |
| novel class 1 integrons and sequence types in vim-2 and vim-11-producing clinical strains of enterobacter cloacae. | all vim-producing enterobacteriaceae (six enterobacter cloacae) submitted to the argentinian reference laboratory in antimicrobial resistance in the period 2008-13 were characterized. the isolates were referred from 6 nosocomial institutions located in 5 different cities across the country. all isolates showed carbapenem disk diffusion inhibition zones ≤22mm and synergism between a carbapenem disk and edta/sma. the six isolates were pcr positive for blavim. imipenem mics were ≤1 to 8μg/ml. typin ... | 2017 | 28739428 |
| novel multifunctional plant growth-promoting bacteria in co-compost of palm oil industry waste. | previously, a unique co-compost produced by composting empty fruit bunch with anaerobic sludge from palm oil mill effluent, which contributed to establishing a zero-emission industry in malaysia. little was known about the bacterial functions during the composting process and fertilization capacity of this co-compost. we isolated 100 strains from the co-compost on 7 types of enumeration media and screened 25 strains using in vitro tests for 12 traits, grouping them according to three functions: ... | 2017 | 28736147 |
| imipenem-resistant gram-negative bacterial isolates carried by persons upon medical examination in korea. | carbapenem-resistant gram-negative bacteria (cr-gnb) have emerged and disseminated worldwide, become a great concern worldwide including korea. the prevalence of fecal carriage of imipenem-resistant gram-negative bacteria (ir-gnb) in persons in korea was investigated. stool samples were collected from 300 persons upon medical examination. samples were screened for ir-gnb by using macconkey agar with 2 μl/ml imipenem. species were identified by 16s rrna gene sequence analysis, and antimicrobial s ... | 2017 | 28721477 |
| introduction of sample tubes with sodium azide as a preservative for ethyl glucuronide in urine. | ethyl glucuronide (etg) is a direct alcohol marker, which is widely used for clinical and forensic applications, mainly for abstinence control. however, the instability of etg in urine against bacterial degradation or the post-collectional synthesis of etg in contaminated samples may cause false interpretation of etg results in urine samples. this study evaluates the potential of sodium azide in tubes used for urine collection to hinder degradation of ethyl glucuronide by bacterial metabolism ta ... | 2017 | 28712037 |
| insights into a novel blakpc-2-encoding incp-6 plasmid reveal carbapenem-resistance circulation in several enterobacteriaceae species from wastewater and a hospital source in spain. | untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria. however, its role in the spread of antibiotic resistances in the human population remains poorly investigated. we used whole genome sequencing to analyze 25 kpc-2-producing enterobacteriaceae isolates from sewage water collected during a 3-year period and three clinical citrobacter freundii isolates from a tertiary hospital in the same collection ar ... | 2017 | 28702005 |
| accelerated detection of extended-spectrum β-lactamases in clinical isolates of enterobacteriaceae. | a prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum β-lactamases (esbls) in clinical isolates of escherichia coli, klebsiella pneumoniae and other gram-negative bacteria. a modified double-disc test (mddt) is incorporated in a gram-negative template for routine susceptibility testing. the mddt identified accurately esbls in all isolates subsequently confirmed as esblproducers by the standard clinical laboratory standards ... | 2006 | 28700883 |
| transglycosylation by a chitinase from enterobacter cloacae subsp. cloacae generates longer chitin oligosaccharides. | humans have exploited natural resources for a variety of applications. chitin and its derivative chitin oligosaccharides (chos) have potential biomedical and agricultural applications. availability of chos with the desired length has been a major limitation in the optimum use of such natural resources. here, we report a single domain hyper-transglycosylating chitinase, which generates longer chos, from enterobacter cloacae subsp. cloacae 13047 (ecchi1). ecchi1 was optimally active at ph 5.0 and ... | 2017 | 28698589 |
| surgical site infection with extended-spectrum β-lactamase-producing enterobacteriaceae after cardiac surgery: incidence and risk factors. | to determine the incidence, microbiology and risk factors for sternal wound infection (swi) with extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-pe) following cardiac surgery. | 2017 | 28698036 |
| investigation and analysis of the characteristics and drug sensitivity of bacteria in skin ulcer infections. | skin ulcer is a common type of disease affecting patients' health and quality of life, and bacterial infection increases the difficulty of its management. | 2017 | 28689800 |
| the oral bacterial microbiome of occlusal surfaces in children and its association with diet and caries. | dental caries is the most prevalent disease in humans globally. efforts to control it have been invigorated by an increasing knowledge of the oral microbiome composition. this study aimed to evaluate the bacterial diversity in occlusal biofilms and its relationship with clinical surface diagnosis and dietary habits. anamneses were recorded from thirteen 12-year-old children. biofilm samples collected from occlusal surfaces of 46 permanent second molars were analyzed by 16s rrna amplicon sequenci ... | 2017 | 28678838 |
| the distribution of carbapenem- and colistin-resistance in gram-negative bacteria from the tamil nadu region in india. | the occurrence of carbapenem- and colistin-resistance among gram-negative bacteria is increasing worldwide. the aim of this study was to understand the distribution of carbapenem- and colistin-resistance in two areas in tamil nadu, india. | 2017 | 28671537 |
| polymicrobial and monomicrobial infections after spinal surgery: a retrospective study to determine which infection is more severe. | a retrospective clinical review. | 2017 | 28670411 |
| whole-genome sequencing overrules a suspected case of carbapenem-resistant enterobacter cloacae transmission. | 2017 | 28659317 | |
| treatment approach for infection of healed fractures after internal fixation. | to review the efficacy of a treatment approach for patients with infection and colonized implants after open reduction and internal fixation of fractures. | 2017 | 28650945 |
| multidrug-resistant enterobacteriaceae in lebanese hospital wastewater: implication in the one health concept. | extended-spectrum beta-lactamases (esbl) and carbapenemase-producing organisms pose severe problems for hospitalized patients. in the absence of efficient sanitation and sewage disposal, the risks for transmission of hospital organisms into the community are high. our objectives were to study the occurrence and mechanisms of resistance of multidrug-resistant gram-negative bacilli in two lebanese hospital sewage treatment plants. wastewater samples were collected, filtered, and cultivated on macc ... | 2017 | 28650688 |
| extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates from neonates in tunisia. | this study was conducted to investigate extended-spectrum-β-lactamase (esbl) producing enterobacteriaceae isolates from the center of maternity and neonatology of monastir, tunisia. fourty-six strains out of 283 were found to produce esbl: klebsiella pneumoniae (n = 37), escherichia coli (n = 6), enterobacter cloacae (n = 2), and citrobacter freundi (n = 1). genotyping analysis, using eric2 and rapd, showed that strains were clonally unrelated. pcr amplification followed by sequencing revealed t ... | 2017 | 28648624 |
| structure and gene cluster of the o-antigen of enterobacter cloacae c4115. | an o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of enterobacter cloacae c4115 and studied by sugar analysis along with 1d and 2d (1)h and (13)c nmr spectroscopy. the following structure of the linear pentasaccharide repeating unit of the o-polysaccharide was established: →2)-α-l-rhap-(1 → 2)-α-l-rhap-(1 → 2)-α-l-rhap-(1 → 2)-α-d-galp-(1 → 3)-α-d-fucpnac-(1→ the o-antigen gene cluster of e. cloacae c4115 was sequenced. the gene functions were tentatively assign ... | 2017 | 28645003 |
| the antimicrobial spectrum of xeroform(®). | xeroform(®) is a petrolatum-based fine mesh gauze containing 3% bismuth tribromophenate. bismuth, similar to other metals, has antimicrobial properties. xeroform(®) has been used for decades in burn and plastic surgery as a donor site dressing and as a covering for wounds or partial thickness burns. despite this, the antimicrobial spectrum of xeroform(®) remains largely unknown. we examined the in-vitro efficacy of xeroform(®) against common burn pathogens using zone-of-inhibition methodology in ... | 2017 | 28641915 |
| successful conservative management of a permanent pacemaker pocket infection: a less invasive approach. | we present a successful conservative management strategy for a frail elderly patient with a cardiac resynchronisation pacemaker who presented with evidence of an enterobacter cloacae pacemaker pocket infection. a device washout and debridement procedure was performed, with reburial of the device in a new prepectoral pocket and creation of a closed-loop continuous antibiotic infusion into the infected pacemaker pocket. this was followed by a 6-week course of ambulatory intravenous antibiotic ther ... | 2017 | 28637845 |
| molecular and microbiological report of a hospital outbreak of ndm-1-carrying enterobacteriaceae in mexico. | to characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant enterobacteriaceae (cre) in a tertiary-care hospital in mexico. | 2017 | 28636666 |
| imipenem/cilastatin : a reappraisal of its antibacterial activity, pharmacokinetic properties and therapeutic efficacy. | imipenem is an antibacterial agent of the carbapenem class of β-lactams, with a very broad spectrum of activity that includes most gram-negative and gram-positive pathogens, aerobes and anaerobes, and with marked activity against species producing β-lactamases. it is coadministered with cilastatin, a renal dehydropeptidase inhibitor that prevents renal metabolism of imipenem. as initial monotherapy, imipenem/cilastatin provides effective and well-tolerated treatment of moderate to severe infecti ... | 1992 | 28634918 |
| fecal carriage of extended-spectrum β-lactamase- and carbapenemase-producing enterobacteriaceae in egyptian patients with community-onset gastrointestinal complaints: a hospital -based cross-sectional study. | the aim of this study was to determine the prevalence of extended-spectrum β-lactamase (esbl) and carbapenemase production among enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to el-ahrar general hospital, zagazig, egypt in the period between january 2013 and may 2013. | 2017 | 28630686 |
| bacterial biofilm-based catheter-associated urinary tract infections: causative pathogens and antibiotic resistance. | we sought to determine the incidence of bacterial biofilm-based catheter-associated urinary tract infections, identify variables affecting biofilm formation, and identify etiologic bacterial pathogens and antibiotic-resistance patterns associated with biofilm-based catheter-associated urinary tract infections (cautis) in our setup. | 2017 | 28629757 |
| a new microfluidic approach for the one-step capture, amplification and label-free quantification of bacteria from raw samples. | a microfluidic method to specifically capture and detect infectious bacteria based on immunorecognition and proliferative power is presented. it involves a microscale fluidized bed in which magnetic and drag forces are balanced to retain antibody-functionalized superparamagnetic beads in a chamber during sample perfusion. captured cells are then cultivated in situ by infusing nutritionally-rich medium. the system was validated by the direct one-step detection of salmonella typhimurium in undilut ... | 2017 | 28626552 |
| aerobic biogenesis of selenium nanoparticles by enterobacter cloacae z0206 as a consequence of fumarate reductase mediated selenite reduction. | in the present study, we examined the ability of enterobacter cloacae z0206 to reduce toxic sodium selenite and mechanism of this process. e. cloacae z0206 was found to completely reduce up to 10 mm selenite to elemental selenium (se°) and form selenium nanoparticles (senps) under aerobic conditions. the selenite reducing effector of e. cloacae z0206 cell was to be a membrane-localized enzyme. itraq proteomic analysis revealed that selenite induced a significant increase in the expression of fum ... | 2017 | 28607388 |
| population pharmacokinetics of tigecycline in critically ill patients with severe infections. | we sought to describe the population pharmacokinetics of tigecycline in critically ill patients and to determine optimized dosing regimens of tigecycline for different bacterial infections. this prospective study included 10 critically ill patients given a standard dose of tigecycline. blood samples were collected during one dosing interval and were analyzed using validated chromatography. population pharmacokinetics and monte carlo dosing simulations were undertaken using pmetrics. three target ... | 2017 | 28607024 |
| fri-2 carbapenemase-producing enterobacter cloacae complex in the uk. | detection of rarer carbapenemases is challenging, as it requires molecular assays with comprehensive coverage or the use of phenotypic methods for the detection of carbapenemase activity. we describe a new class a carbapenemase, fri-2, in an enterobacter cloacae complex isolate following implementation of an in-house multiplex pcr for the detection of 'rare' class a carbapenemases. | 2017 | 28605515 |
| the epidemiology, management, and outcomes of bacterial meningitis in infants. | the pathogens that cause bacterial meningitis in infants and their antimicrobial susceptibilities may have changed in this era of increasing antimicrobial resistance, use of conjugated vaccines, and maternal antibiotic prophylaxis for group b streptococcus (gbs). the objective was to determine the optimal empirical antibiotics for bacterial meningitis in early infancy. | 2017 | 28600447 |
| draft genome sequences of enterobacter cloacae strains caprex e7 and caprex e2-2. | enterobacter cloacae strains caprex e7 and caprex e2-2 were isolated from ghanaian yams at a london market. the draft genome sequences indicate that the two strains are similar, with genomes of 5,042,838 and 5,039,930 bp and 56.19% and 55.05% g+c content, respectively. both strains encoded three different β-lactamases, including one of the ampc family. | 2017 | 28596406 |
| combination antimicrobial therapy for bacterial infections : guidelines for the clinician. | therapy with antimicrobial combinations has been used as long as antimicrobials have been available. combinations of antibiotics are often used to take advantage of different mechanisms of action and/or toxicity profiles. well established indications for combination antimicrobial therapy include: (a) empirical treatment of life-threatening infections; (b) treatment of polymicrobial infections; (c) prevention of the emergence of bacterial resistance; and (d) for synergism. disadvantages of combin ... | 1996 | 28593588 |
| genetic and biochemical characterisation of ctx-m-37 extended-spectrum β-lactamase from an enterobacter cloacae clinical isolate from mongolia. | the aims of this study were to determine the resistance level of a blactx-m-37-carrying enterobacter cloacae isolate from mongolia, to analyse kinetic parameters of the purified enzyme and to compare the genetic environment of the gene. | 2017 | 28587869 |
| microbial decolorization and detoxification of emerging environmental pollutant: cosmetic hair dyes. | since the usage of hair dyes has increased in recent time, the removal of residual dye from environment is also an emerging issue. hair dye contains mixture of chemicals including genotoxic chemical, p-phenylenediamine (p-pd or ppd). the present study reports bioremediation of hair dye using bacteria isolated from saloon effluent. sugarcane bagasse powder (sbp) was used as a source of nutrient and surface for bacterial growth. the 16s rdna sequencing confirmed the isolate as enterobacter cloacae ... | 2017 | 28586750 |
| enterobacter cloacae-related necrotizing fasciitis after peritoneal dialysis in delayed graft function: a case report. | necrotizing fasciitis is an uncommon soft-tissue infection that involves the superficial fascia, subcutaneous fat, and deep fascia. herein, we report the first case of enterobacter cloacae-related necrotizing fasciitis after peritoneal dialysis in delayed graft function. | 2017 | 28583554 |
| mechanism-informed refinement reveals altered substrate-binding mode for catalytically competent nitroreductase. | nitroreductase (nr) from enterobacter cloacae reduces diverse nitroaromatics including herbicides, explosives, and prodrugs, and holds promise for bioremediation, prodrug activation, and enzyme-assisted synthesis. we solved crystal structures of nr complexes with bound substrate or analog for each of its two half-reactions. we complemented these with kinetic isotope effect (kie) measurements elucidating h-transfer steps essential to each half-reaction. kies indicate hydride transfer from nadh to ... | 2017 | 28578873 |
| multilocus sequence typing of carbapenemase-producing enterobacter cloacae bloodstream isolates between 2002 and 2013 in a taiwanese university hospital. | 2017 | 28576741 | |
| mcr-1 plasmid-mediated colistin resistance gene detection in an enterobacter cloacae clinical isolate in france. | 2017 | 28576739 | |
| outbreak of ctx-m-15-producing enterobacter cloacae associated with therapeutic beds and syphons in an intensive care unit. | an outbreak of extended-spectrum β-lactamase-producing enterobacter cloacae (esbl-ecl) occurred in our intensive care unit (icu) and involved 18 patients (8 infected and 10 colonized). the mean age of patients was 69 years, and all infected patients had underlying medical conditions. within hours' recognition of the spread of esbl-ecl, the infection control team requested for staff education, reinforcement of infection control measures, and environmental screening. new transmissions were observe ... | 2017 | 28571981 |
| molecular and functional characterization of myxobacteria isolated from soil in india. | this study reports the isolation of myxobacteria from soil collected from plains in north india. based on the morphology and 16s rdna sequence, the isolated myxobacteria were identified as corallococcus sp., pyxidicoccus sp., myxococcus sp., cystobacter sp. and archangium sp. the myxobacteria were functionally characterized to assess their ability to produce antibacterial and anticancer metabolites. the isolates were found to be functionally versatile as they produced extracellular bioactive mol ... | 2017 | 28567623 |
| occurrence of plasmid- and chromosome-carried mcr-1 in waterborne enterobacteriaceae in china. | the aim of this study was to investigate the prevalence of the polymyxin resistance gene mcr-1 in enterobacteriaceae from environmental water sources in hangzhou, china. colistin-resistant bacteria were isolated from environmental water samples using an enrichment broth culture method, were screened for mcr-1, and then were analyzed for the location and transferability of mcr-1 isolates positive for mcr-1 were further examined to determine their susceptibility profiles and were screened for the ... | 2017 | 28559252 |
| total hip prosthesis complication, periprosthetic infection with external fistulizing due to enterobacter cloacae complex multiple drugs resistance: a clinical case report. | the enterobacter cloacae is a microorganism found in the intestinal flora of the majority of animals, including humans. primary infections caused by e. cloacae are rare in immunocompetent patients, but are very common in hospital settings in newborns and immunocompromised patients, and can be aggravated by the insurgence of antibiotic resistance. the incidence of periprosthetic hip infections is just below 2%. | 2017 | 28558347 |
| outbreak of enterobacter cloacae with new delhi metallo-β-lactamase (ndm)-1: challenges in epidemiological investigation and environmental decontamination. | 2017 | 28552405 | |
| multiple brain abscesses due to enterobacter cloacae in an immune-competent child. | brain abscesses due to enterobacteriaceae in immune-competent children are rare, and those due to enterobacter cloacae are even rarer. we report an interesting case of community-acquired e. cloacae neuroinfection resulting in multiple brain abscesses in a young child with no underlying risk-factors. a 10 year-old-boy presented with low-grade fever, headache, neck pain and progressive deterioration of sensorium. on examination, he was conscious but drowsy with photophobia, normal fundii, meningea ... | 2017 | 28545899 |
| epidemiological, clinical and microbiological characteristics of patients with post-traumatic osteomyelitis of limb fractures in southwest china: a hospital-based study. | objective to determine the epidemiological, clinical and microbiological characteristics, of patients with post-traumatic osteomyelitis of extremity fractures, and provide evidence-based guidelines for early diagnosis and treatment, including empiric antibiotic therapy. methods human subject research was performed using institutional review board approved protocols. a retrospective chart review was conducted on 5,368 patients diagnosed with extremity traumatic fractures from january 1, 2012 to d ... | 2017 | 28540152 |
| synthesis of β-galactooligosaccharide using bifidobacterial β-galactosidase purified from recombinant escherichia coli. | galactooligosaccharides (gos) are known to be selectively utilized by bifidobacterium, which can bring about healthy changes of the composition of intestinal microflora. in this study, β-gos are synthesized using bifidobacterial β-galactosidase(g1) purified from recombinant e. coli with a high gos yield and with high productivity and enhanced bifidogenic activity. the purified recombinant g1 showed maximum production of β-gos at ph 8.5 and 45°c. a matrix-assisted laser desorption ionization time ... | 2017 | 28535609 |
| genomic epidemiology of global vim-producing enterobacteriaceae. | international data on the molecular epidemiology of enterobacteriaceae with vim carbapenemases are limited. | 2017 | 28520983 |
| bioremoval of lead using pennisetum purpureum augmented with enterobacter cloacae-vitpasj1: a pot culture approach. | lead is a toxic heavy metal discharged into the ecosystem from various industries. biological remediation strategies have been effective in the bioremoval of lead. in our current study, a phytobacterial system using pennisetum purpureum along with lead-resistant bacterium (lrb) was employed for the uptake of lead. the lrb was obtained from lead-contaminated sites. the isolate vitpasj1 was found to be highly tolerant to lead and was identified as an effective plant growth-promoting bacterium. the ... | 2017 | 28509939 |
| a metabonomic analysis on the response of enterobacter cloacae from coastal outfall for land-based pollutant under phoxim stress. | enterobacter cloacae is an opportunistic pathogen widely distributed in human and animal intestinal systems. the secretion of extended-spectrum β-lactamases (esbls) and cephalosporinase (ampc) endows e. cloacae with strong drug resistance. in a previous study by our group, protein expression of e. cloacae under phoxim stress was measured by two-dimensional electrophoresis. here, nuclear magnetic resonance was used to detect differences in e. cloacae metabonomics when under phoxim stress. we dete ... | 2017 | 28508092 |
| microbiological analysis of acute infections of the nail fold on the basis of bait thread test. | an acute infection of the nail fold, called paronychia, is a common clinical problem. the basis for the implementation of the treatment is the result of microbiological examination. due to the rapid and painful course of infection, usually an empirical antimicrobial treatment prior to obtaining microbiological test results is introduced. | 2017 | 28507488 |
| applications of microalgal biofilms for wastewater treatment and bioenergy production. | microalgae have shown clear advantages for the production of biofuels compared with energy crops. apart from their high growth rates and substantial lipid/triacylglycerol yields, microalgae can grow in wastewaters (animal, municipal and mining wastewaters) efficiently removing their primary nutrients (c, n, and p), heavy metals and micropollutants, and they do not compete with crops for arable lands. however, fundamental barriers to the industrial application of microalgae for biofuel production ... | 2017 | 28491136 |
| metagenomic sequencing of diamondback moth gut microbiome unveils key holobiont adaptations for herbivory. | herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. our previous study of the genome of diamondback moth, plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. here we expand on that work with a complete taxonomic and functi ... | 2017 | 28491055 |
| evaluation of the carbapenem inactivation method (cim) for detecting carbapenemase activity in enterobacteria. | the objective of this study was to evaluate the accuracy of the cim test in the detection of carbapenemase activity in 124 strains of enterobacteriaceae. | 2017 | 28476242 |
| clinical and molecular epidemiology of carbapenem-resistant enterobacteriaceae among adult inpatients in singapore. | since 2010, the incidence of carbapenem-resistant enterobacteriaceae (cre) has been increasing in singapore. we analyzed the clinical and molecular epidemiology of cre among adult inpatients in singapore. | 2017 | 28475792 |
| usefulness of matrix-assisted laser desorption ionization time-of-flight mass spectrometry to identify pathogens, including polymicrobial samples, directly from blood culture broths. | matrix-assisted laser desorption ionization time-of-flight mass spectrometry (tof-ms) is now widely used to detect pathogens in clinical settings in japan. here, we report the ability of tof-ms to detect bacteria from blood culture (bc) broths, and compare the efficacy of tof-ms to that of conventional culture methods. bacteria were correctly detected from 63 monomicrobial samples within 80 minutes; results matched those obtained by conventional bc methods, although the conventional methods took ... | 2017 | 28458566 |
| structure, genetics and worldwide spread of new delhi metallo-β-lactamase (ndm): a threat to public health. | the emergence of carbapenemase producing bacteria, especially new delhi metallo-β-lactamase (ndm-1) and its variants, worldwide, has raised amajor public health concern. ndm-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria. | 2017 | 28449650 |
| transfer of kpc-2 carbapenemase from klebsiella pneumoniae to enterobacter cloacae in a patient receiving meropenem therapy. | the horizontal transfer of a plasmid bearing the blakpc-2 gene from k. pneumoniae to e. cloacae infecting the respiratory tract of a patient during meropenem therapy was elucidated. this finding is particularly worrisome, since these drugs are of last resort for multidrug-resistant gram-negative pathogens. | 2017 | 28442305 |
| novel monoclonal antibodies against stx1d and 1e and their use for improving immunoassays. | shiga toxins (stxs) are major causative agents for bloody diarrhea and hemolytic uremic syndrome, a life-threatening disease in humans. no effective treatment is available. early detection of stxs in clinical samples is critical for disease management. as bacteria evolve, new stxs are produced; therefore, methods used to identify them need to be improved as well. in this study, new monoclonal antibodies (mabs) against stx1d and 1e were developed and used to improve a commercial stx1 kit. incorpo ... | 2017 | 28434982 |
| a comparison of molecular typing methods applied to enterobacter cloacae complex: hsp60 sequencing, rep-pcr, and mlst. | molecular typing using repetitive sequenced-based pcr (rep-pcr) and hsp60 sequencing were applied to a collection of diverse enterobacter cloacae complex isolates. to determine the most practical method for reference laboratories, we analyzed 71 e. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. while rep-pcr was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus seq ... | 2017 | 28428984 |
| allodemic distribution of plasmids co-harbouring blactx-m-15/aac(6')-ib-cr/qnrb in klebsiella pneumoniae is the main source of extended-spectrum β-lactamases in uruguay's paediatric hospital. | this study aimed to describe the characteristics of clinical isolates of extended-spectrum β-lactamase (esbl)-producing enterobacteria (epe) in uruguay's paediatric hospital. | 2017 | 28419869 |
| carbapenem-resistant enterobacter cloacae in patients from the us veterans health administration, 2006-2015. | we analyzed carbapenem-resistant enterobacteriaceae (cre) trends among patients from the us veterans health administration (vha). after the emergence of cre in the eastern united states, resistance rates remained stable in klebsiella pneumoniae but increased in enterobacter cloacae complex, suggesting a "second epidemic". vha offers a vantage point for monitoring nationwide cre trends. | 2017 | 28418318 |
| what the eyes don't see, the heart does grieve over. | a 71 year old peruvian woman presented with blurry vision and bilateral eye pain and had evidence of bilateral endophthalmitis on eye examination. her past medical history was notable for multiple abdominal surgeries within the preceding months, including an incarcerated inguinal hernia repair which required an ileostomy placement, and cholecystitis requiring cholecystostomy tube placement. over her multiple long hospitalizations, she developed bacteremia and fungemia on several occasions, with ... | 2017 | 28414678 |
| antimicrobial activity and chemical composition of brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide. | brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. however, studies on the antimicrobial activity of brunfelsia uniflora flower oleoresin have not been found yet. this study aimed to evaluate the chemical composition and antimicrobial activity of b. uniflora flower oleoresin obtained by supercritical carbon dioxide. oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition ... | 2017 | 28407186 |
| sequencing and comparative genomics analysis of the inchi2 plasmids pt5282-mpha and p112298-cata and the inchi5 plasmid pynkp001-dfra. | incompatibility group inchi plasmids are important vectors of antibiotic resistance in enterobacteriaceae. in this study, a scheme for typing inchi into five separately clustering subgroups, including previously designated inchi1-3 as well as inchi4-5, was proposed based on sequenced inchi plasmids. the complete nucleotide sequences of the inchi2 plasmids pt5282-mpha and p112298-cata and the inchi5 plasmid pynkp001-dfra from clinical enterobacter cloacae, citrobacter freundii and raoultella orni ... | 2017 | 28390961 |
| the draft genome sequence of the plant growth promoting rhizospheric bacterium enterobacter cloacae sbp-8. | here, we report the draft genome sequence and annotation of plant growth promoting rhizobacterium enterobacter cloacae sbp-8 isolated from the rhizosphere of sorghum bicolor l. growing in desert region of rajasthan, india. from the genome sequences, we identified the genes encoding plant growth promoting properties such as 1-aminocyclopropane-1-carboxylate deaminase (acds), phosphate solubilisation, siderophore, and iaa (indole acetic acid) production. the genes encoding different functions requ ... | 2017 | 28386532 |
| draft genome sequence of a high-level colistin-resistant clinical strain of the enterobacter cloacae complex. | strain wchecl-c4 of the enterobacter cloacae complex, recovered from the blood of a patient with peritonitis, was high-level resistant to colistin. here, we report its 5.1-mb draft genome sequence, comprising 92 contigs with an average 55.74% g+c content. the genome contained 4,783 coding sequences and 68 trna genes. | 2017 | 28385849 |
| carbapenemase-producing enterobacteriaceae recovered from a spanish river ecosystem. | the increasing resistance to carbapenems is an alarming threat in the fight against multiresistant bacteria. the dissemination properties of antimicrobial resistance genes are supported by their detection in a diverse population of bacteria, including strains isolated from the environment. the objective of this study was to investigate the presence of carbapenemase-producing enterobacteriaceae (cpe) collected from a river ecosystem in the barcelona metropolitan area (spain). identification of β- ... | 2017 | 28380016 |
| [community acquired urinary tract infections in older adults]. | background community acquired urinary tract infections (cauti) in adults are common ailments. older adults are prone to infectious diseases, diagnosis can be difficult, their etiologic and antimicrobial resistance are poorly known. objectives to evaluate the incidence discriminated by sex, symptoms, and to determine pathogens and their antimicrobial resistance rate. methods a retrospective, descriptive and comparative study. we analyze uricultures among 2013 in the hospital nacional de clinicas ... | 2017 | 28379126 |
| xer site specific recombination: double and single recombinase systems. | the separation and segregation of newly replicated bacterial chromosomes can be constrained by the formation of circular chromosome dimers caused by crossing over during homologous recombination events. in escherichia coli and most bacteria, dimers are resolved to monomers by site-specific recombination, a process performed by two chromosomally encoded tyrosine recombinases (xerc and xerd). xercd recombinases act at a 28 bp recombination site dif, which is located at the replication terminus reg ... | 2017 | 28373867 |
| activity of cefiderocol (s-649266) against carbapenem-resistant gram-negative bacteria collected from inpatients in greek hospitals. | cefiderocol (s-649266), a siderophore cephalosporin, utilizes a novel mechanism of entry into the periplasmic space of gram-negative bacteria and is broadly stable to esbls and carbapenemases. | 2017 | 28369471 |
| gallbladder microbiota variability in colombian gallstones patients. | gallbladder stones are a very frequently occurring condition. despite bile bactericidal activity, many bacteria have been detected inside the gallbladder, and gallstones facilitate their presence. between 3% and 5% of the patients with salmonella spp. infection develop the carrier stage, with the bacteria persisting inside the gallbladder, shedding bacteria in their feces without signs of infection. the aim of this study was to isolate bacteria from colombian patients with gallstones, using stan ... | 2017 | 28368860 |
| efficient synthesis of tyrosol galactosides by the β-galactosidase from enterobacter cloacae b5. | in this work, the β-galactosidase from enterobacter cloacae b5 (bgab5) exhibited excellent transglycosylation activity toward tyrosol (p-hydroxyphenethyl alcohol) when using lactose as the glycosyl donor, generating a series of tyrosol glycosides with potential pharmacological properties. the effects of substrate concentration, temperature, ph, and reaction time on the transglycosylation reaction catalyzed by the enzyme bgab5 were studied in detail. three tyrosol derivatives were produced in a t ... | 2017 | 28361236 |
| troubled water under the bridge: screening of river mur water reveals dominance of ctx-m harboring escherichia coli and for the first time an environmental vim-1 producer in austria. | antibiotic resistant bacteria (arb) in the aquatic environment are reported from all over the world and their presence in the environment has become quite common. the current most prominent example is the presence of beta-lactamases harboring enterobacteriaceae. the aim of this study was to investigate the presence and diversity (on the genetic and phenotypic levels) of extended spectrum beta-lactamases (esbl) and carbapenemases harboring enterobacteriaceae from the river mur in the center of gr ... | 2017 | 28351808 |
| prevalence and antimicrobial susceptibility of enterobacteriaceae isolated from retail pepper in vietnam. | to investigate the microbial quality of retail pepper in vietnam, the enumeration and detection of enterobacteriaceae and the screening of cefotaxime (ctx)-resistant coliforms were performed by using 84 commercial samples. although enterobacteriaceae were isolated from 78 samples, the number of enterobacteriaceae was lower than 1.0 log cfu/g in 46 samples. for the detection of enterobacteriaceae with the international organization for standardization methods, salmonella spp., escherichia coli , ... | 2017 | 28350183 |
| n6', n6''', and o4' modifications to neomycin affect ribosomal selectivity without compromising antibacterial activity. | the synthesis of a series of neomycin derivatives carrying the 2-hydroxyethyl substituent on n6' and/or n6‴ both alone and in combination with a 4'-o-ethyl group is described. by means of cell-free translation assays with wild-type bacterial ribosomes and their hybrids with eukaryotic decoding a sites, we investigate how individual substituents and their combinations affect activity and selectivity at the target level. in principle, and as shown by cell-free translation assays, modifications of ... | 2017 | 28343384 |