Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| isolation of two ochrobactrum sp. strains capable of degrading the nootropic drug-piracetam. | piracetam (2-oxo-1-pyrrolidine acetamide) is a popular cognitive enhancer, which has recently been detected in waste and drinking water. nootropic drugs are designed to affect human metabolism and act on the nervous system, but their environmental effects have yet to be the subject of detailed studies. in this report, we present the efficient biodegradation of the cognitive enhancer, piracetam. two bacterial strains capable of using this compound as the sole carbon source were isolated and later ... | 2017 | 28720419 |
| ochrobactrum anthropi fulminant early-onset neonatal sepsis, a case report and review of literature. | ochrobactrum anthropi is gram-negative bacteria that cause infection in humans, particularly immunocompromised patients and those with indwelling central venous catheters. o. anthropi is unlikely to cause fulminant sepsis in infected patients. a few cases of late-onset neonatal sepsis have been reported in preterm infants with congenital anomalies. we hereby present the first published case of fulminant early-onset neonatal sepsis in a premature newborn. | 2017 | 28654560 |
| ochrobactrum anthropi-caused osteomyelitis in the foot mimicking a bone tumor: case report and review of the literature. | osteomyelitis due to ochrobactrum anthropi, a new genus ochrobacterum widely distributed in the environment and occasionally associated with human infection, has been described in only a few case reports. we present a report of an unusual case of osteomyelitis caused by o. anthropi that was identified 9 years after a nail puncture to the lateral cuneiform bone. the patient was an 18-year-old male with a painful foot lesion that had originally been misdiagnosed as an osteolytic tumor. he underwen ... | 2017 | 28633790 |
| highly sensitive bacteriophage-based detection of brucella abortus in mixed culture and spiked blood. | for decades, bacteriophages (phages) have been used for brucella species identification in the diagnosis and epidemiology of brucellosis. traditional brucella phage typing is a multi-day procedure including the isolation of a pure culture, a step that can take up to three weeks. in this study, we focused on the use of brucellaphages for sensitive detection of the pathogen in clinical and other complex samples, and developed an indirect method of brucella detection using real-time quantitative pc ... | 2017 | 28604602 |
| hypertonic saline as a useful tool for sputum induction and pathogen detection in cystic fibrosis. | the aim of this study was to compare the qualitative and semi-quantitative detection of pathogens in the airway secretions of patients with cystic fibrosis (cf) and the sputum induction capacity before and after inhalation of 7% hypertonic saline solution (hss). | 2017 | 28455785 |
| isolation of viable but non-culturable bacteria from printing and dyeing wastewater bioreactor based on resuscitation promoting factor. | printing and dyeing wastewater with high content of organic matters, high colority, and poor biochemical performance is hard to be degraded. in this study, we isolated viable but non-culturable (vbnc) bacteria from printing and dyeing wastewater with the culture media contained resuscitation promoting factor (rpf) protein secreted by micrococcus luteus, counted the culturable cells number with the most probable number, sequenced 16s rrna genes, and performed polymerase chain reaction-denaturing ... | 2017 | 28417187 |
| biodegradation of used engine oil by novel strains of ochrobactrum anthropi hm-1 and citrobacter freundii hm-2 isolated from oil-contaminated soil. | used engine oil (ueo) constitutes a serious environmental problem due to the difficulty of disposal off or reuse. ten bacterial strains with biodegradation potential were isolated from ueo-contaminated soil sample using enrichment technique. two strains which exhibited the highest degradation %, 51 ± 1.2 and 48 ± 1.5, respectively, were selected. based on the morphological, biochemical characteristics and 16s rrna sequence analysis, they were identified as ochrobactrum anthropi hm-1 (accession n ... | 2016 | 28330298 |
| brucella spp. of amphibians comprise genomically diverse motile strains competent for replication in macrophages and survival in mammalian hosts. | twenty-one small gram-negative motile coccobacilli were isolated from 15 systemically diseased african bullfrogs (pyxicephalus edulis), and were initially identified as ochrobactrum anthropi by standard microbiological identification systems. phylogenetic reconstructions using combined molecular analyses and comparative whole genome analysis of the most diverse of the bullfrog strains verified affiliation with the genus brucella and placed the isolates in a cluster containing b. inopinata and th ... | 2017 | 28300153 |
| biodegradation of benzo[a]pyrene by bacterial consortium isolated from mangrove sediment. | benzo[a]pyrene is a high-molecular-weight polycyclic aromatic hydrocarbon highly recalcitrant in nature and thus harms the ecosystem and/or human health. therefore, its removal from the marine environment is crucial. this research focuses on benzo[a]pyrene degradation by using enriched bacterial isolates in consortium under saline conditions. bacterial isolates capable of using benzo[a]pyrene as sole source of carbon and energy were isolated from enriched mangrove sediment. these isolates were i ... | 2017 | 28281885 |
| organophosphonates utilization by soil strains of ochrobactrum anthropi and achromobacter sp. | four bacterial strains from glyphosate- or alkylphosphonates-contaminated soils were tested for ability to utilize different organophosphonates. all studied strains readily utilized methylphosphonic acid and a number of other phosphonates, but differed in their ability to degrade glyphosate. only strains ochrobactrum anthropi gpk 3 and achromobacter sp. kg 16 utilized this compound after isolation from enrichment cultures with glyphosate. achromobacter sp. mpk 7 from the same enrichment culture, ... | 2017 | 28184965 |
| genome sequence of ochrobactrum anthropi strain subg007, a plant pathogen and potential xenobiotic compounds degradation bacterium. | ochrobactrum anthropi subg007 was isolated from the fruit of prunus dulcis in rajkot (22.30°n, 70.78°e), gujarat, india. here we present the 4.37 mb genome sequence strain subg007, which may provide the genetic information for the application in environment pollution degradation and agriculture field. the strain also posses many genes cluster which involved in production of important secondary metabolites. the nucleotide sequence of this genome was deposited into ncbi genbank under the accession ... | 2017 | 28119820 |
| advances in maize transformation technologies and development of transgenic maize. | maize is the principal grain crop of the world. it is also the crop where genetic engineering has been employed to a great extent to improve its various traits. the ability to transform maize is a crucial step for application of gene technology in maize improvement. there have been constant improvements in the maize transformation technologies over past several years. the choice of genotype and the explant material to initiate transformation and the different types of media to be used in various ... | 2016 | 28111576 |
| advances in maize transformation technologies and development of transgenic maize. | maize is the principal grain crop of the world. it is also the crop where genetic engineering has been employed to a great extent to improve its various traits. the ability to transform maize is a crucial step for application of gene technology in maize improvement. there have been constant improvements in the maize transformation technologies over past several years. the choice of genotype and the explant material to initiate transformation and the different types of media to be used in various ... | 2016 | 28111576 |
| improvement of whole-cell transamination with saccharomyces cerevisiae using metabolic engineering and cell pre-adaptation. | whole-cell biocatalysis based on metabolically active baker's yeast with engineered transamination activity can be used to generate molecules carrying a chiral amine moiety. a prerequisite is though to express efficient ω-transaminases and to reach sufficient intracellular precursor levels. | 2017 | 28049528 |
| the change of a medically important genus: worldwide occurrence of genetically diverse novel brucella species in exotic frogs. | the genus brucella comprises various species of both veterinary and human medical importance. all species are genetically highly related to each other, sharing intra-species average nucleotide identities (ani) of > 99%. infections occur among various warm-blooded animal species, marine mammals, and humans. until recently, amphibians had not been recognized as a host for brucella. in this study, however, we show that novel brucella species are distributed among exotic frogs worldwide. comparative ... | 2016 | 28036367 |
| isolation and characterisation of crude oil sludge degrading bacteria. | the use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. polycyclic aromatic hydrocarbons (pahs), as the major components of oil sludge, are hydrophobic and recalcitrant. an important way of enhancing the rate of pah desorption is to compost crude oil sludge by incorporating commercial surfactants, thereby making them available for microbial degradation. in this ... | 2016 | 27933233 |
| a study of pre-operative presence of micro-organisms in affected knee joints of rheumatoid arthritis patients who need total knee arthroplasty. | to evaluate, by way of intraoperative tissue culture and pathological study, the pre-operative presence of micro-organisms in knee joints of patients with rheumatoid arthritis (ra) who need total knee arthroplasty (tka). | 2017 | 27919672 |
| biochemical and structural analysis of a novel esterase from caulobacter crescentus related to penicillin-binding protein (pbp). | considering that the prevalence of antibiotic-resistant pathogenic bacteria is largely increasing, a thorough understanding of penicillin-binding proteins (pbps) is of great importance and crucial significance because this enzyme family is a main target of β-lactam-based antibiotics. in this work, combining biochemical and structural analysis, we present new findings that provide novel insights into pbps. here, a novel pbp homologue (ccesta) from caulobacter crescentus cb15 was characterized usi ... | 2016 | 27905486 |
| a t4ss effector targets host cell alpha-enolase contributing to brucella abortus intracellular lifestyle. | brucella abortus, the causative agent of bovine brucellosis, invades and replicates within cells inside a membrane-bound compartment known as the brucella containing vacuole (bcv). after trafficking along the endocytic and secretory pathways, bcvs mature into endoplasmic reticulum-derived compartments permissive for bacterial replication. brucella type iv secretion system (virb) is a major virulence factor essential for the biogenesis of the replicative organelle. upon infection, brucella uses t ... | 2016 | 27900285 |
| ochromobactrum intermedium: an emerging opportunistic pathogen-case of recurrent bacteraemia associated with infective endocarditis in a haemodialysis patient. | we describe the first clinical case report of infective endocarditis related to ochrobactrum intermedium infection. the case involved a 23-year-old man receiving dialysis via an internal jugular long-term haemodialysis catheter. he improved with a prolonged course of meropenem and minocycline. ochrobactrum spp. are recognized as rare emerging opportunistic pathogens. | 2016 | 27843545 |
| ochromobactrum intermedium: an emerging opportunistic pathogen-case of recurrent bacteraemia associated with infective endocarditis in a haemodialysis patient. | we describe the first clinical case report of infective endocarditis related to ochrobactrum intermedium infection. the case involved a 23-year-old man receiving dialysis via an internal jugular long-term haemodialysis catheter. he improved with a prolonged course of meropenem and minocycline. ochrobactrum spp. are recognized as rare emerging opportunistic pathogens. | 2016 | 27843545 |
| molecular detection and characterization of brucella species in raw informally marketed milk from uganda. | this study identified and characterized brucella species in the informal milk chain in uganda. a total of 324 cattle bulk milk samples were screened for the genus brucella by real-time pcr with primers targeting the bcsp31 gene and further characterized by the omp25 gene. of the samples tested, 6.5% were positive for brucella species. in the omp25 phylogeny, the study sequences were found to form a separate clade within the branch containing b. abortus sequences. the study shows that informally ... | 2016 | 27839533 |
| genomic comparison of agrobacterium pusense strains isolated from bean nodules. | 2016 | 27833604 | |
| halotolerant rhizobacteria promote growth and enhance salinity tolerance in peanut. | use of plant growth promoting rhizobacteria (pgpr) is a promising strategy to improve the crop production under optimal or sub-optimal conditions. in the present study, five diazotrophic salt tolerant bacteria were isolated from the roots of a halophyte, arthrocnemum indicum. the isolates were partially characterized in vitro for plant growth promoting traits and evaluated for their potential to promote growth and enhanced salt tolerance in peanut. the 16s rrna gene sequence homology indicated t ... | 2016 | 27790198 |
| isolation of a novel 'atypical' brucella strain from a bluespotted ribbontail ray (taeniura lymma). | a pleomorphic gram-negative, motile coccobacillus was isolated from the gills of a wild-caught bluespotted ribbontail ray after its sudden death during quarantine. strain 141012304 was observed to grow aerobically, to be clearly positive for cytochrome oxidase, catalase, urease and was initially identified as "brucella melitensis" or "ochrobactrum anthropi" by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and vitek2-compact(®), respectively. affiliation to the genu ... | 2017 | 27785661 |
| ochrobactrum anthropi keratitis with focal descemet's membrane detachment and intracorneal hypopyon. | purpose. to describe a unique case of o. anthropi keratitis associated with a rare manifestation of descemet's membrane detachment and intracorneal hypopyon and to discuss challenges in diagnosis and management. methods. best-corrected visual acuity was measured with snellen letters. corneal scrapings were performed and aerobic, viral, herpetic, acid-fast bacilli, acanthamoeba, and fungal stains and cultures were obtained. following evisceration, tissue was evaluated for histologic features and ... | 2016 | 27777806 |
| depth and well type related to groundwater microbiological contamination. | use of groundwater from private wells in households has increased considerably, owing to a better cost/benefit ratio than that of water provided by local utilities for a fee. however, this water is usually untreated, which makes it a vehicle for diseases. thus, monitoring this water is necessary to ensure its integrity and quality. we aimed to evaluate the physical, chemical, and microbiological parameters of untreated groundwater drawn from different types of wells, and the antimicrobial suscep ... | 2016 | 27775681 |
| temperature-dependent regulation of the ochrobactrum anthropi proteome. | ochrobactrum anthropi is a gram-negative rod belonging to the brucellaceae family, able to colonize a variety of environments, and actually reported as a human opportunistic pathogen. despite its low virulence, the bacterium causes a growing number of hospital-acquired infections mainly, but not exclusively, in immunocompromised patients. the aim of this study was to obtain an overview of the global proteome changes occurring in o. anthropi in response to different growth temperatures, in order ... | 2016 | 27753207 |
| a brucella spp. isolate from a pac-man frog (ceratophrys ornata) reveals characteristics departing from classical brucellae. | brucella are highly infectious bacterial pathogens responsible for brucellosis, a frequent worldwide zoonosis. the brucella genus has recently expanded from 6 to 11 species, all of which were associated with mammals; the natural host range recently expanded to amphibians after some reports of atypical strains from frogs. here we describe the first in depth phenotypic and genetic characterization of a brucella strains isolated from a frog. strain b13-0095 was isolated from a pac-man frog (ceratop ... | 2016 | 27734009 |
| pyrethroid-degrading microorganisms and their potential for the bioremediation of contaminated soils: a review. | pyrethroid insecticides have been used to control pests in agriculture, forestry, horticulture, public health and for indoor home use for more than 20 years. because pyrethroids were considered to be a safer alternative to organophosphate pesticides (ops), their applications significantly increased when the use of ops was banned or limited. although, pyrethroids have agricultural benefits, their widespread and continuous use is a major problem as they pollute the terrestrial and aquatic environm ... | 2016 | 27695449 |
| characterization of crude oil degrading bacteria isolated from contaminated soils surrounding gas stations. | a total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. of the 20 isolates, ram03, ram06, ram13, and ram17 were specifically chosen based on their relatively higher growth on salt medium amended with 4 % crude oil, emulsion index, surface tension, and degradation percentage. these bacterial cultures had 16s rrna gene sequences that were most similar to ochrobactrum cytisi (ram03), ochrobactrum anthropi (ram06 and ram17), and sinorhizobium meliloti (ram13) with 96 % ... | 2016 | 27655077 |
| identification and characterization of cefotaxime resistant bacteria in beef cattle. | third-generation cephalosporins are an important class of antibiotics that are widely used in treatment of serious gram-negative bacterial infections. in this study, we report the isolation of bacteria resistant to the third-generation cephalosporin cefotaxime from cattle with no previous cefotaxime antibiotic exposure. the prevalence of cefotaxime-resistant bacteria was examined by a combination of culture based and molecular typing methods in beef cattle (n = 1341) from 8 herds located in nort ... | 2016 | 27642751 |
| succinyl-coa synthetase: new antigen candidate of bartonella bacilliformis. | bartonella bacilliformis is the causative agent of carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. the lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. this study aimed to identify new b. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas. | 2016 | 27627803 |
| gst activity and membrane lipid saturation prevents mesotrione-induced cellular damage in pantoea ananatis. | callisto(®), containing the active ingredient mesotrione (2-[4-methylsulfonyl-2-nitrobenzoyl]1,3-cyclohenanedione), is a selective herbicide that controls weeds in corn crops and is a potential environmental contaminant. the objective of this work was to evaluate enzymatic and structural changes in pantoea ananatis, a strain isolated from water, in response to exposure to this herbicide. despite degradation of mesotrione, probably due a glutathione-s-transferase (gst) pathway in pantoea ananatis ... | 2016 | 27620734 |
| structural differences in gut bacteria communities in developmental stages of natural populations of lutzomyia evansi from colombia's caribbean coast. | lutzomyia evansi, a phlebotomine insect endemic to colombia's caribbean coast, is considered to be the main vector of visceral and cutaneous leishmaniasis in the region. although insects of this species can harbor pathogenic and non-pathogenic microorganisms in their intestinal microbiota, there is little information available about the diversity of gut bacteria present in lutzomyia evansi. in this study, conventional microbiological methods and molecular tools were used to assess the compositio ... | 2016 | 27618991 |
| utility of ochrobactrum anthropi yc152 in a microbial fuel cell as an early warning device for hexavalent chromium determination. | fast hexavalent chromium (cr(vi)) determination is important for environmental risk and health-related considerations. we used a microbial fuel cell-based biosensor inoculated with a facultatively anaerobic, cr(vi)-reducing, and exoelectrogenic ochrobactrum anthropi yc152 to determine the cr(vi) concentration in water. the results indicated that o. anthropi yc152 exhibited high adaptability to ph, temperature, salinity, and water quality under anaerobic conditions. the stable performance of the ... | 2016 | 27537887 |
| the use of pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) to detect bacterial and fungal colonization in healthy military service members. | the role of microbial colonization in disease is complex. novel molecular tools to detect colonization offer theoretical improvements over traditional methods. we evaluated pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) as a screening tool to study colonization of healthy military service members. | 2016 | 27448413 |
| beneficial cyanobacteria and eubacteria synergistically enhance bioavailability of soil nutrients and yield of okra. | microorganisms in the rhizosphere mediate the cycling of nutrients, their enhanced mobilisation and facilitate their uptake, leading to increased root growth, biomass and yield of plants. we examined the promise of beneficial cyanobacteria and eubacteria as microbial inoculants, applied singly or in combination as consortia or biofilms, to improve growth and yields of okra. interrelationships among the microbial activities and the micro/macro nutrient dynamics in soils and okra yield characteris ... | 2016 | 27441245 |
| outbreak of achromobacter xylosoxidans and ochrobactrum anthropi infections after prostate biopsies, france, 2014. | we report an outbreak of healthcare-associated prostatitis involving rare environmental pathogens in immunocompetent patients undergoing transrectal prostate biopsies at hôpital édouard herriot (lyon, france) during august 13-october 10, 2014. despite a fluoroquinolone-based prophylaxis, 5 patients were infected with achromobacter xylosoxidans and 3 with ochrobactrum anthropi, which has not been reported as pathogenic in nonimmunocompromised persons. all patients recovered fully. analysis of the ... | 2016 | 27434277 |
| chronic postoperative endophthalmitis after cataract surgery secondary to vancomycin-resistant ochrobactrum anthropi: case report and literature review. | the aim of this study was to report an unusual case of chronic postoperative endophthalmitis following cataract surgery, secondary to ochrobactrum anthropi that was found to be resistant to vancomycin. | 2016 | 27421273 |
| a case of ochrobactrum anthropi-induced septic shock and infective endocarditis. | ochrobactrum anthropi is a gram-negative rod of low virulence. infections due to this organism are uncommon; however in immunocompromised hosts it can cause severe infections. among the many infections it can cause, infective endocarditis is very rare. even rarer is infective endocarditis of the native valves, as ochrobactrum antropi affects damaged or prosthetic valves almost exclusively. this case describes native valve endocarditis due to ochrobactrum anthropi. [full article available at http ... | 2016 | 27379356 |
| isolation of bdellovibrio bacteriovorus from a tropical wastewater treatment plant and predation of mixed species biofilms assembled by the native community members. | it is reported here that a predatory bacterium belonging to the genus bdellovibrio, was isolated from activated sludge at the ulu pandan water reclamation plant, singapore. 16s rdna gene sequencing analysis revealed that this isolate was 99% identical to 'bdellovibrio bacteriovorus strain tiberius' and hence is designated as 'bdellovibrio bacteriovorus up'. using a novel approach based on fluorescence in situ hybridization (fish), a prey cell density-dependent growth pattern of b. bacteriovorus ... | 2016 | 27328268 |
| genomics of high molecular weight plasmids isolated from an on-farm biopurification system. | the use of biopurification systems (bps) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. bps environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (mges), which play a key role in bacterial adaptation and evolution in such environments. here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-fa ... | 2016 | 27321040 |
| relationship between intrauterine bacterial infection and early embryonic developmental arrest. | early embryonic developmental arrest is the most commonly understudied adverse outcome of pregnancy. the relevance of intrauterine infection to spontaneous embryonic death is rarely studied and remains unclear. this study aimed to investigate the relationship between intrauterine bacterial infection and early embryonic developmental arrest. | 2016 | 27270541 |
| brucella suis bacteremia misidentified as ochrobactrum anthropi by the vitek 2 system. | ochrobactrum and brucella are genetically related genera of the family brucellaceae, sharing 98.8% rrna similarity. because of their phenotypic similarity, ochrobactrum can be miscoded as brucella by automated identification systems. the misidentification on blood cultures (bcs) of b. suis as o. anthropi by the vitek 2 system is herein described. a 67-year-old male with a prosthetic mitral valve and fever was admitted with bacteremia due to a gram-negative coccobacillus identified as o. anthropi ... | 2016 | 27131010 |
| draft genome sequence of organophosphate-degrading ochrobactrum anthropi fraf13. | italic! ochrobactrum anthropifraf13 was isolated from farmland soil in jersey village, texas. fraf13 is a bacterial microorganism with broad antibiotic resistance that possesses a number of metal-dependent β-lactam enzymes with secondary phosphotriesterase activity that can initiate the breakdown of organophosphate compounds. | 2016 | 27103720 |
| vim-47, a new variant of the autochthonous metallo-β-lactamase vim-13 from the balearic islands in spain. | 2016 | 26976859 | |
| isolation, fingerprinting and genetic identification of indigenous pahs degrading bacteria from oil-polluted soils. | in the present study, thirty five bacterial isolates were obtained from hydrocarbon-contaminated soil samples using an enrichment method. these isolates were tested to grow on mineral salt medium containing anthracene or phenanthrene as sole carbon source. only five isolates showed the ability to degrade these compounds. rapd-pcr fingerprinting was carried out for the five isolates, and the dna patterns revealed that there was no similarity among the examined bacteria whenever the rflp using fou ... | 2016 | 26930863 |
| highly diverse nirk genes comprise two major clades that harbour ammonium-producing denitrifiers. | copper dependent nitrite reductase, nirk, catalyses the key step in denitrification, i.e. nitrite reduction to nitric oxide. distinct structural nirk classes and phylogenetic clades of nirk-type denitrifiers have previously been observed based on a limited set of nirk sequences, however, their environmental distribution or ecological strategies are currently unknown. in addition, environmental nirk-type denitrifiers are currently underestimated in pcr-dependent surveys due to primer coverage lim ... | 2016 | 26923558 |
| first case report of human infection with ochrobactrum tritici causing bacteremia and cholecystitis. | 2016 | 26915622 | |
| metagenomic sequencing of the chronic obstructive pulmonary disease upper bronchial tract microbiome reveals functional changes associated with disease severity. | chronic obstructive pulmonary disease (copd) is a major source of mortality and morbidity worldwide. the microbiome associated with this disease may be an important component of the disease, though studies to date have been based on sequencing of the 16s rrna gene, and have revealed unequivocal results. here, we employed metagenomic sequencing of the upper bronchial tract (ubt) microbiome to allow for greater elucidation of its taxonomic composition, and revealing functional changes associated w ... | 2016 | 26872143 |
| isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane. | endophytic nitrogen fixing bacteria were isolated from the leaves, stems and roots of industrial variety (cv. u-thong 3; ut3), wild and chewing sugarcane plants grown for 6 weeks in nitrogen (n)-free sand. eighty nine isolates of endophytic bacteria were obtained on n-free agar. an acetylene reduction assay (ara) detected nitrogenase activity in all 89 isolates. three isolates from the chewing (c2hl2, c7hl1 and c34mr1) sugarcane and one isolate from the industrial sugarcane (ut3r1) varieties wer ... | 2015 | 26868592 |
| the staphylococcus aureus methicillin resistance factor fmta is a d-amino esterase that acts on teichoic acids. | the methicillin resistance factor encoded by fmta is a core member of the staphylococcus aureus cell wall stimulon, but its function has remained elusive for the past two decades. first identified as a factor that affects methicillin resistance in s. aureus strains, fmta was later shown to interact with teichoic acids and to localize to the cell division septum. we have made a breakthrough in understanding fmta function. we show that fmta hydrolyzes the ester bond between d-ala and the backbone ... | 2016 | 26861022 |
| analysis of the first temperate broad host range brucellaphage (bipbo1) isolated from b. inopinata. | brucella species are important human and animal pathogens. though, only little is known about mobile genetic elements of these highly pathogenic bacteria. to date, neither plasmids nor temperate phages have been described in brucellae. we analyzed genomic sequences of various reference and type strains and identified a number of putative prophages residing within the brucella chromosomes. by induction, phage bipbo1 was isolated from brucella inopinata. bipbo1 is a siphovirus that infects several ... | 2016 | 26858702 |
| consistent 1,3-propanediol production from glycerol in mixed culture fermentation over a wide range of ph. | glycerol is currently an over-produced chemical that can be used as substrate for the production of high value products such as 1,3-propanediol (1,3-pdo) in fermentation processes. the aim of this study was to investigate the effect of initial ph on a batch mixed culture fermentation of glycerol, considering both the bacterial community composition and the fermentation patterns. | 2016 | 26855671 |
| microbial glycolipoprotein-capped silver nanoparticles as emerging antibacterial agents against cholera. | with the increased number of cholera outbreaks and emergence of multidrug resistance in vibrio cholerae strains it has become necessary for the scientific community to devise and develop novel therapeutic approaches against cholera. recent studies have indicated plausibility of therapeutic application of metal nano-materials. among these, silver nanoparticles (agnps) have emerged as a potential antimicrobial agent to combat infectious diseases. at present nanoparticles are mostly produced using ... | 2016 | 26829922 |
| structural understanding of the recycling of oxidized ascorbate by dehydroascorbate reductase (osdhar) from oryza sativa l. japonica. | dehydroascorbate reductase (dhar) is a key enzyme involved in the recycling of ascorbate, which catalyses the glutathione (gsh)-dependent reduction of oxidized ascorbate (dehydroascorbate, dha). as a result, dhar regenerates a pool of reduced ascorbate and detoxifies reactive oxygen species (ros). in previous experiments involving transgenic rice, we observed that overexpression of dhar enhanced grain yield and biomass. since the structure of dhar is not available, the enzymatic mechanism is not ... | 2016 | 26775680 |
| bloodstream infections after solid-organ transplantation. | solid-organ transplantation (sot) has become the preferred strategy to treat a number of end-stage organ disease, because a continuous improvement in survival and quality of life. while preventive strategies has decreased the risk for classical opportunistic infections (such as viral, fungal and parasite infections), bacterial infections, and particularly bloodstream infections (bsis) remain the most common and life-threatening complications in sot recipients. the source of bsi after transplant ... | 2016 | 26766415 |
| a novel naturally occurring class i 5-enolpyruvylshikimate-3-phosphate synthase from janibacter sp. confers high glyphosate tolerance to rice. | as glyphosate is a broad spectrum herbicide extensively used in agriculture worldwide, identification of new aroa genes with high level of glyphosate tolerance is essential for the development and breeding of transgenic glyphosate-tolerant crops. in this study, an aroa gene was cloned from a janibacter sp. strain isolated from marine sediment (designated as aroaj. sp). the purified aroaj. sp enzyme has a km value of 30 μm for pep and 83 μm for s3p, and a significantly higher ki value for glyphos ... | 2016 | 26754957 |
| prevalence and antimicrobial resistance of microbes causing bloodstream infections in unguja, zanzibar. | bloodstream infections (bsi) are frequent and cause high case-fatality rates. urgent antibiotic treatment can save patients' lives, but antibiotic resistance can render antibiotic therapy futile. this study is the first to collect epidemiological data on bsi from unguja, zanzibar. | 2015 | 26700032 |
| [research progress of ochrobactrum anthropic--a review]. | ochrobactrum anthropi is a gram-negative bacillus, usually known as an opportunistic pathogen. mostly its infection is related with systemic or local lower immunity, and manifested as bacteremia, meningitis, purulent infection. recently, along with expanded infection, it has become an important human pathogen. the prevention, clinical diagnose and treatment become complicated because varied clinical symptoms increased antibiotic resistance and cross immune reaction with others pathogens. in this ... | 2015 | 26665594 |
| microbiological testing of pharmaceuticals and cosmetics in egypt. | microbial contamination of pharmaceuticals poses a great problem to the pharmaceutical manufacturing process, especially from a medical as well as an economic point of view. depending upon the product and its intended use, the identification of isolates should not merely be limited to the united states pharmacopeia (usp) indicator organisms. | 2015 | 26653032 |
| a population-based acute meningitis and encephalitis syndromes surveillance in guangxi, china, may 2007-june 2012. | acute meningitis and encephalitis (ame) are common diseases with the main pathogens being viruses and bacteria. as specific treatments are different, it is important to develop clinical prediction rules to distinguish aseptic from bacterial or fungal infection. in this study we evaluated the incidence rates, seasonal variety and the main etiologic agents of ame, and identified factors that could be used to predict the etiologic agents. | 2015 | 26633824 |
| [undulant fever and autoimmune hemolytic anemia in a 20-year-old exchange student from jordan - the human brucellosis as an important differential diagnosis in migrants]. | a 20-year-old jordanian exchange student presents with recurrent fever, night sweats, cough, and swelling and redness around the ankle. physical examination further reveals bilateral ankle arthritis and painful cervical lymphadenopathy. | 2015 | 26625239 |
| fluorescent multiple staining and casa system to assess boar sperm viability and membranes integrity in short and long-term extenders. | the aim of this study was to assess the effect on boar spermatozoa quality of in vitro storage in short and long-term extenders by fluorescent multiple staining (fms) and computer assisted semen analyzer (casa). fresh ejaculates from three healthy, sexually mature boars were diluted with equal volumes of six short-term or three long-term commercial extenders and stored at 19°c for 6 days (short-term) or 12 days (long-term). the integrity of spermatozoa membranes was analyzed by fms using propidi ... | 2013 | 26623308 |
| ochrobactrum endophyticum sp. nov., isolated from roots of glycyrrhiza uralensis. | a novel gram-staining negative, motile, rod-shaped and aerobic bacterial strain, designated egi 60010(t), was isolated from healthy roots of glycyrrhiza uralensis f. collected from yili county, xinjiang province, north-west china. the 16s rrna gene sequence of strain egi 60010(t) showed 97.2 % sequence similarities with ochrobactrum anthropi atcc 49188(t) and ochrobactrum cytisi esc1(t), and 97.1 % with ochrobactrum lupini lup21(t). the phylogenetic analysis based on 16s rrna gene sequences show ... | 2016 | 26615404 |
| a simple and safe protocol for preparing brucella samples for matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis. | we describe a simple protocol to inactivate the biosafety level 3 (bsl3) pathogens brucella prior to their analysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. this method is also effective for several other bacterial pathogens and allows storage, and eventually shipping, of inactivated samples; therefore, it might be routinely applied to unidentified bacteria, for the safety of laboratory workers. | 2016 | 26582837 |
| bacteria associated with amblyomma cajennense tick eggs. | ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. in the americas, the ixodidae ticks amblyomma cajennense are responsible for severe impact on livestock and public health. in the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with a. cajennense eggs from females sampled in distinct geographical sites in southeastern brazil. additional comparative analysis of the culturable bacteria from an ... | 2015 | 26537602 |
| bacteria that travel: the quality of aircraft water. | the travelling population is increasing globally year on year. international tourist arrival figures reached 1087 million in 2013 and 1133 million in 2014; of which 53% and 54% respectively accounted for air transport. the water on board aircraft is sourced from surface or ground water; piped to a central filling point and distributed to each aircraft by water service vehicles at the home base or at the destination airport. the purpose of this study was to ascertain the microbial, chemical (ph; ... | 2015 | 26529000 |
| type iv secretion system of brucella spp. and its effectors. | brucella spp. are intracellular bacterial pathogens that cause infection in domestic and wild animals. they are often used as model organisms to study intracellular bacterial infections. brucella virb t4ss is a key virulence factor that plays important roles in mediating intracellular survival and manipulating host immune response to infection. in this review, we discuss the roles of brucella virb t4ss and 15 effectors that are proposed to be crucial for brucella pathogenesis. virb t4ss regulate ... | 2015 | 26528442 |
| perspective for aquaponic systems: "omic" technologies for microbial community analysis. | aquaponics is the combined production of aquaculture and hydroponics, connected by a water recirculation system. in this productive system, the microbial community is responsible for carrying out the nutrient dynamics between the components. the nutrimental transformations mainly consist in the transformation of chemical species from toxic compounds into available nutrients. in this particular field, the microbial research, the "omic" technologies will allow a broader scope of studies about a cu ... | 2015 | 26509157 |
| isolation and characterization of a crude oil degrading bacteria from formation water: comparative genomic analysis of environmental ochrobactrum intermedium isolate versus clinical strains. | in this study, we isolated an environmental clone of ochrobactrum intermedium, strain 2745-2, from the formation water of changqing oilfield in shanxi, china, which can degrade crude oil. strain 2745-2 is aerobic and rod-shaped with optimum growth at 42 °c and ph 5.5. we sequenced the genome and found a single chromosome of 4 800 175 bp, with a g+c content of 57.63%. sixty rnas and 4737 protein-coding genes were identified: many of the genes are responsible for the degradation, emulsification, a ... | 2015 | 26465134 |
| general stress signaling in the alphaproteobacteria. | the alphaproteobacteria uniquely integrate features of two-component signal transduction and alternative σ factor regulation to control transcription of genes that ensure growth and survival across a range of stress conditions. research over the past decade has led to the discovery of the key molecular players of this general stress response (gsr) system, including the sigma factor σ(ecfg), its anti-σ factor nepr, and the anti-anti-σ factor phyr. the central molecular event of gsr activation ent ... | 2015 | 26442844 |
| homeostasis and its disruption in the lung microbiome. | the disciplines of physiology and ecology are united by the shared centrality of the concept of homeostasis: the stability of a complex system via internal mechanisms of self-regulation, resilient to external perturbation. in the past decade, these fields of study have been bridged by the discovery of the lung microbiome. the respiratory tract, long considered sterile, is in fact a dynamic ecosystem of microbiota, intimately associated with the host inflammatory response, altered in disease stat ... | 2015 | 26432870 |
| metabolic response of clostridium ljungdahlii to oxygen exposure. | clostridium ljungdahlii is an important synthesis gas-fermenting bacterium used in the biofuels industry, and a preliminary investigation showed that it has some tolerance to oxygen when cultured in rich mixotrophic medium. batch cultures not only continue to grow and consume h2, co, and fructose after 8% o2 exposure, but fermentation product analysis revealed an increase in ethanol concentration and decreased acetate concentration compared to non-oxygen-exposed cultures. in this study, the mech ... | 2015 | 26431975 |
| novel real-time pcr detection assay for brucella suis. | brucella suis is the causative agent of brucellosis in suidae and is differentiated into five biovars (bv). biovars 1 and 3 possess zoonotic potential and can infect humans, whereas biovar 2 represents the main source of brucellosis in feral and domestic pigs in europe. both aspects, the zoonotic threat and the economic loss, emphasize the necessity to monitor feral and domestic pig populations. available serological or pcr based methods lack sensitivity and specificity. | 2015 | 26392898 |
| rhodococcus erythropolis bg43 genes mediating pseudomonas aeruginosa quinolone signal degradation and virulence factor attenuation. | rhodococcus erythropolis bg43 is able to degrade the pseudomonas aeruginosa quorum sensing signal molecules pqs (pseudomonas quinolone signal) [2-heptyl-3-hydroxy-4(1h)-quinolone] and hhq [2-heptyl-4(1h)-quinolone] to anthranilic acid. based on the hypothesis that degradation of hhq might involve hydroxylation to pqs followed by dioxygenolytic cleavage of the heterocyclic ring and hydrolysis of the resulting n-octanoylanthranilate, the genome was searched for corresponding candidate genes. two g ... | 2015 | 26319870 |
| lamp technology: rapid identification of brucella and mycobacterium avium subsp. paratuberculosis. | in this study, we developed new sets of primers to detect brucella spp. and m. avium subsp. paratuberculosis (map) through isothermal amplification. we selected a previously well-characterized target gene, bscp31, specific for brucella spp. and is900 for map. the limits of detection using the loop-mediated isothermal amplification (lamp) protocols described herein were similar to those of conventional pcr targeting the same sequences. hydroxynaphtol blue and sybr green(tm) allowed direct naked-e ... | 2015 | 26273282 |
| quality control testing for tracking endotoxin-producing gram-negative bacteria during the preparation of polyvalent snake antivenom immunoglobulin. | snake bites represent a serious public health problem, particularly in rural areas worldwide. antitoxic sera preparations are antibodies from immunized animals and are considered to be the only treatment option. the purification of antivenom antibodies should aim at obtaining products of consistent quality, safety, efficacy, and adherence to good manufacturing practice principles. endotoxins are an integral component of the outer cell surface of gram-negative bacteria. they are common contaminat ... | 2017 | 26242786 |
| active-site engineering of ω-transaminase for production of unnatural amino acids carrying a side chain bulkier than an ethyl substituent. | ω-transaminase (ω-ta) is a promising enzyme for use in the production of unnatural amino acids from keto acids using cheap amino donors such as isopropylamine. the small substrate-binding pocket of most ω-tas permits entry of substituents no larger than an ethyl group, which presents a significant challenge to the preparation of structurally diverse unnatural amino acids. here we report on the engineering of an (s)-selective ω-ta from ochrobactrum anthropi (oata) to reduce the steric constraint ... | 2015 | 26231640 |
| ochrobactrum anthropi induced retropharyngeal abscess with mediastinal extension complicating airway obstruction: a case report. | retropharyngeal abscess with involvement of mediastinal abscess represents an uncommon complication of upper respiratory tract infections. we report a case presenting with a large retropharyngeal abscess with airway obstruction as the primary presenting symptom. contrast-enhanced ct showed a large retropharyngeal abscess in the neck with extension to the upper and posterior mediastinal spaces. the abscess was surgically excised with 200 cc pus drained from the neck and mediastinal regions. we de ... | 2014 | 26229647 |
| draft genome sequence of ochrobactrum anthropi strain w13p3, a halotolerant polycyclic aromatic hydrocarbon-degrading bacterium. | ochrobactrum anthropi w13p3 was isolated from saline soil contaminated by polycyclic aromatic hydrocarbons (pahs) and could degrade pahs with 5% nacl. we report the 5.3-mb draft genome sequence of this strain, which is helpful for understanding the diversity of ochrobactrum spp. and the mechanism of pah degradation in saline environments. | 2015 | 26227610 |
| high voltage electrochemiluminescence (ecl) as a new method for detection of pah during screening for pah-degrading microbial consortia. | the search for new bacterial consortia capable of removing pah from the environment is associated with the need to employ novel, simple, and economically efficient detection methods. a fluorimetric method (fl) as well as high voltage electrochemiluminescence (ecl) on a modified surface of an aluminum electrode were used in order to determine the changes in the concentrations of pah in the studied aqueous solutions. the ecl signal (the spectrum and emission intensity for a given wavelength) was d ... | 2015 | 26213425 |
| in silico identification for α-amino-ε-caprolactam racemases by using information on the structure and function relationship. | in silico identification for enzymes having desired functions is attractive because there is a possibility that numerous desirable enzymes have been deposited in databases. in this study, α-amino-ε-caprolactam (acl) racemases were searched from the ncbi protein database. four hundred thirteen fold-type i pyridoxal 5'-phosphate-dependent enzymes which are considered to contain sequences of acl racemase were firstly obtained by submitting the sequence of acl racemase from achromobacter obae to the ... | 2015 | 26206345 |
| the enrichment of an alkaliphilic biofilm consortia capable of the anaerobic degradation of isosaccharinic acid from cellulosic materials incubated within an anthropogenic, hyperalkaline environment. | anthropogenic hyperalkaline sites provide an environment that is analogous to proposed cementitious geological disposal facilities (gdf) for radioactive waste. under anoxic, alkaline conditions cellulosic wastes will hydrolyze to a range of cellulose degradation products (cdp) dominated by isosaccharinic acids (isa). in order to investigate the potential for microbial activity in a cementitious gdf, cellulose samples were incubated in the alkaline (∼ph 12), anaerobic zone of a lime kiln waste si ... | 2015 | 26195600 |
| first isolation and characterization of brucella microti from wild boar. | brucella microti was first isolated from common vole (microtus arvalis) in the czech republic in central europe in 2007. as b. microti is the only brucella species known to live in soil, its distribution, ecology, zoonotic potential, and genomic organization is of particular interest. the present paper is the first to report the isolation of b. microti from a wild boar (sus scrofa), which is also the first isolation of this bacterial species in hungary. | 2015 | 26163135 |
| recent advances in brucella abortus vaccines. | brucella abortus vaccines play a central role in bovine brucellosis control/eradication programs and have been successfully used worldwide for decades. strain 19 and rb51 are the approved b. abortus vaccines strains most commonly used to protect cattle against infection and abortion. however, due to some drawbacks shown by these vaccines much effort has been undertaken for the development of new vaccines, safer and more effective, that could also be used in other susceptible species of animals. ... | 2015 | 26155935 |
| development of a loop-mediated isothermal amplification method for the rapid detection of the dioxin-degrading bacterium ochrobactrum anthropi in soil. | in this study, loop-mediated isothermal amplification (lamp) and real-time lamp assays were developed to detect the dioxin-degrading bacterium ochrobactrum anthropi strain bd-1 in soil. four primers were designed to use its gene amplification for the strain o. anthropi bd-1. the real-time lamp assay was found to accomplish the reaction by 1 pg of genomic dna load when used for nucleic acid amplification. this assay was then applied to detect o. anthropi bd-1 in eight soil samples collected from ... | 2015 | 26144562 |
| reduction of hexavalent chromium by the thermophilic methanogen methanothermobacter thermautotrophicus. | despite the significant progress on iron reduction by thermophilic microorganisms, studies on their ability to reduce toxic metals are still limited, despite their common co-existence in high temperature environments (up to 70°c). in this study, methanothermobacter thermautotrophicus, an obligate thermophilic methanogen, was used to reduce hexavalent chromium. experiments were conducted in a growth medium with h2/co2 as substrate with various cr(6+) concentrations (0.2, 0.4, 1, 3, and 5 mm) in t ... | 2014 | 26120143 |
| reduction of hexavalent chromium by the thermophilic methanogen methanothermobacter thermautotrophicus. | despite the significant progress on iron reduction by thermophilic microorganisms, studies on their ability to reduce toxic metals are still limited, despite their common co-existence in high temperature environments (up to 70°c). in this study, methanothermobacter thermautotrophicus, an obligate thermophilic methanogen, was used to reduce hexavalent chromium. experiments were conducted in a growth medium with h2/co2 as substrate with various cr(6+) concentrations (0.2, 0.4, 1, 3, and 5 mm) in t ... | 2014 | 26120143 |
| phenotypic and phylogenetic identification of coliform bacteria obtained using 12 coliform methods approved by the u.s. environmental protection agency. | the current definition of coliform bacteria is method dependent, and when different culture-based methods are used, discrepancies in results can occur and affect the accuracy of identification of true coliforms. this study used an alternative approach to the identification of true coliforms by combining the phenotypic traits of the coliform isolates and the phylogenetic affiliation of 16s rrna gene sequences with the use of lacz and uida genes. a collection of 1,404 isolates detected by 12 u.s. ... | 2015 | 26116679 |
| identification of highly pathogenic microorganisms by matrix-assisted laser desorption ionization-time of flight mass spectrometry: results of an interlaboratory ring trial. | in the case of a release of highly pathogenic bacteria (hpb), there is an urgent need for rapid, accurate, and reliable diagnostics. maldi-tof mass spectrometry is a rapid, accurate, and relatively inexpensive technique that is becoming increasingly important in microbiological diagnostics to complement classical microbiology, pcr, and genotyping of hpb. in the present study, the results of a joint exercise with 11 partner institutions from nine european countries are presented. in this exercise ... | 2015 | 26063856 |
| the medical diagnostic approaches with phylogenetic analysis for rare brucella spp. diagnosis in taiwan. | brucellosis is a bacterial zoonotic disease which can be easy to misdiagnose in clinical microbiology laboratories. in the present study, we have tried to improve the current clinical method for detecting brucella spp. and its antibiotic characteristics. our method begins with detecting the clinical isolate through traditional biochemical methods and automatic identification systems. then, we move on to editing the sequence for blast allows us to compare 16s rrna sequences with sequences from ot ... | 2015 | 26048695 |
| alterations of lung microbiota in a mouse model of lps-induced lung injury. | acute lung injury (ali) and the more severe acute respiratory distress syndrome are common responses to a variety of infectious and noninfectious insults. we used a mouse model of ali induced by intratracheal administration of sterile bacterial wall lipopolysaccharide (lps) to investigate the changes in innate lung microbiota and study microbial community reaction to lung inflammation and barrier dysfunction induced by endotoxin insult. one group of c57bl/6j mice received lps via intratracheal i ... | 2015 | 25957290 |
| brucella abortus induces the premature death of human neutrophils through the action of its lipopolysaccharide. | most bacterial infections induce the activation of polymorphonuclear neutrophils (pmns), enhance their microbicidal function, and promote the survival of these leukocytes for protracted periods of time. brucella abortus is a stealthy pathogen that evades innate immunity, barely activates pmns, and resists the killing mechanisms of these phagocytes. intriguing clinical signs observed during brucellosis are the low numbers of brucella infected pmns in the target organs and neutropenia in a proport ... | 2015 | 25946018 |
| bacteria may have multiple replication origins. | 2015 | 25941523 | |
| extensive identification of bacterial riboflavin transporters and their distribution across bacterial species. | riboflavin, the precursor for the cofactors flavin mononucleotide (fmn) and flavin adenine dinucleotide, is an essential metabolite in all organisms. while the functions for de novo riboflavin biosynthesis and riboflavin import may coexist in bacteria, the extent of this co-occurrence is undetermined. the ribm, ribn, rfuabcd and the energy-coupling factor-ribu bacterial riboflavin transporters have been experimentally characterized. in addition, impx, rfnt and ribxy are proposed as riboflavin tr ... | 2015 | 25938806 |
| invasive group b streptococcal infection in infants in shenzhen, china. | in this study, we aim to investigate the distribution and antibiotic susceptibility of group b streptococcus (gbs) in infants younger than 90 days in shenzhen, china. | 2015 | 25932259 |
| whole genome sequence comparison of ten diagnostic brucellaphages propagated on two brucella abortus hosts. | recently the genome sequences of two brucellaphages, isolated in georgia (tb) and mexico (pr) were reported revealing pronounced sequence homogeneity and the presence of two major indels discriminating the two phages. subsequent genome sequencing of six diagnostic brucellaphages: tbilisi (tb), firenze (fz), weybridge (wb), s708, berkeley (bk) and r/c phages identified three major genetic groups. however, the propensity for fine-scale genetic variability of diverse brucellaphages grown on multipl ... | 2015 | 25896365 |
| conjunctival flora of clinically normal and diseased turtles and tortoises. | in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises. | 2015 | 25889261 |