Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| prevalence and risk factors for thermotolerant species of campylobacter in poultry meat at retail in europe. | the thermotolerant species campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis are the causative agents of the human illness called campylobacteriosis. this infection represents a threat for the health of consumers in europe. it is well known that poultry meat is an important food vehicle of campylobacter infection. as emerged from the reported scientific literature published between 2006 and 2016, poultry meat sold at retail level in europe represents an i ... | 2017 | 28854745 |
| first isolation and molecular characterisation of a cryptic plasmid from urease-negative campylobacter lari. | 2006 | 28705132 | |
| transmembrane motions of pglb induced by llo are coupled with el5 loop conformational changes necessary for ost activity. | n-linked glycosylation is an enzymatic reaction where an oligosaccharide is transferred en bloc onto an asparagine residue of an acceptor polypeptide, catalyzed by oligosaccharyltransferase (ost). despite the available crystal structures, the role of the external loop el5, which is critical for the catalytic cycle, is still enigmatic as el5 in the crystal structures is partially absent or blocks a pathway of lipid-linked oligosaccharide to the active site. here we report the molecular origin of ... | 2017 | 28575441 |
| prevalence of campylobacter species in wild birds of south korea. | campylobacter species cause human gastrointestinal infections worldwide. they commonly inhabit intestines of avian species including wild birds. they might play a role in the spread of infections to humans and other bird species. the prevalence of campylobacter species in 2164 faecal samples of wild birds (representing 71 species and 28 families) captured across the korean peninsula was evaluated in this study. the overall prevalence was 15.3% (332/2164). bird species belonging to the family cha ... | 2017 | 28503965 |
| simultaneous identification of campylobacter jejuni, campylobacter coli, and campylobacter lari with smartcycler-based multiplex quantitative polymerase chain reaction. | consumption of campylobacter contaminated food or water is a leading cause of human acute gastroenteritis. campylobacter jejuni, campylobacter coli, and campylobacter lari account for over 95% of total campylobacter infections. a multiplex quantitative polymerase chain reaction (qpcr) for simultaneous identification of c. jejuni, c. coli, and c. lari was developed for use with the smartcycler ii system. | 2017 | 28440688 |
| isolation, identification and differentiation of campylobacter spp. using multiplex pcr assay from goats in khartoum state, sudan. | the aim of this study was to identify and characterize thermophilic campylobacter species in faecal samples from goats in khartoum state, sudan, by application of multiplex polymerase chain reaction. campylobacteriosis is a zoonotic disease of global concern, and the organisms can be transmitted to human via food, water and through contact with farm animals and pets. there are five clinically related campylobacter species: campylobacter jejuni (c. jejuni). campylobacter coli, campylobacter lari, ... | 2017 | 28160162 |
| campylobacter ornithocola sp. nov., a new member of the campylobacter lari group isolated from wild bird faecal samples. | during a study on the prevalence and diversity of campylobacteria in wild birds faecal samples in the city of valdivia (south of chile) 17 gram-negative, curved rod isolates, were initially identified as campylobacter lari by pcr-rflp. further identification by 16s rrna sequence analysis revealed that they formed a distinct group in the genus campylobacter. this unique position was confirmed by rpob, atpa and cpn60 gene sequence analysis. the average nucleotide identity between the representativ ... | 2017 | 28126040 |
| isolation of campylobacter spp. from three species of antarctic penguins in different geographic locations. | the presence of campylobacter species was studied in three antarctic penguin species, adélie (pygoscelis adeliae), chinstrap (pygoscelis antarctica) and gentoo (pygoscelis papua). a total of 390 penguins were captured in 12 different rookeries along the antarctic peninsula with differences in the amount of human visitation: six colonies were highly visited [stranger point, king george island (p. papua and p. adeliae); hannah point, livingston island (p. papua and p. antarctica); deception island ... | 2017 | 28091764 |
| advances and challenges in viability detection of foodborne pathogens. | foodborne outbreaks are a serious public health and food safety concern worldwide. there is a great demand for rapid, sensitive, specific, and accurate methods to detect microbial pathogens in foods. conventional methods based on cultivation of pathogens have been the gold standard protocols; however, they take up to a week to complete. molecular assays such as polymerase chain reaction (pcr), sequencing, microarray technologies have been widely used in detection of foodborne pathogens. among mo ... | 2016 | 27920757 |
| isolation of campylobacter spp. from client-owned dogs and cats, and retail raw meat pet food in the manawatu, new zealand. | campylobacter causes acute gastroenteritis in people worldwide and is frequently isolated from food, animals and the environment. the disease is predominately food-borne but many routes of transmission and sources of infection have been described, including contact with pets. the prevalence of campylobacter spp. in dogs and cats varies widely, and data on new zealand pets are limited. this study aimed to investigate the prevalence of campylobacter spp. in dogs, cats and retail raw meat pet food ... | 2016 | 27860343 |
| development of a droplet digital polymerase chain reaction for rapid and simultaneous identification of common foodborne pathogens in soft cheese. | dairy products can harbor various microorganisms (e.g., campylobacter spp., salmonella spp., listeria monocytogenes, verocytotoxin-producing escherichia coli) arising from animal reservoirs, and which can become important sources of foodborne illness. therefore, early detection of food pathogens is crucial to prevent diseases. we wished to develop an accurate quantitative protocol based on a droplet digital polymerase chain reaction (ddpcr) involving eight individual taqman™ reactions to detect ... | 2016 | 27840628 |
| momp from campylobacter jejuni is a trimer of 18-stranded β-barrel monomers with a ca(2+) ion bound at the constriction zone. | the gram-negative organism campylobacter jejuni is the major cause of food poisoning. unlike escherichia coli, which has two major porins, ompc and ompf, c. jejuni has one, termed major outer membrane protein (momp) through which nutrients and antibiotics transit. we report the 2.1-å crystal structure of c. jejuni momp expressed in e. coli and a lower resolution but otherwise identical structure purified directly from c. jejuni. the 2.1-å resolution structure of recombinant momp showed that alth ... | 2016 | 27693650 |
| triclosan resistome from metagenome reveals diverse enoyl acyl carrier protein reductases and selective enrichment of triclosan resistance genes. | triclosan (tcs) is a widely used antimicrobial agent and tcs resistance is considered to have evolved in diverse organisms with extensive use of tcs, but distribution of tcs resistance has not been well characterized. functional screening of the soil metagenome in this study has revealed that a variety of target enoyl acyl carrier protein reductases (enr) homologues are responsible for the majority of tcs resistance. diverse enrs similar to 7-α-hydroxysteroid dehydrogenase (7-α-hsdh), fabg, or t ... | 2016 | 27577999 |
| evaluation of a microfluidic chip system for preparation of bacterial dna from swabs, air, and surface water samples. | the detection of bacterial pathogens from complex sample matrices by pcr requires efficient dna extraction. in this study, a protocol for extraction and purification of dna from swabs, air, and water samples using a microfluidic chip system was established. the optimized protocol includes a combination of thermal, chemical and enzymatic lysis followed by chip-based dna purification using magnetic particles. the procedure was tested using gram-positive bacillus thuringiensis berliner var. kurstak ... | 2016 | 27520284 |
| seroprevalence of campylobacter-specific antibodies in two german duck farms - a prospective follow-up study. | several studies have shown that about 60-100% of farmed ducks are colonized by campylobacter species. because of this, a higher risk of campylobacteriosis among duck farm workers can be assumed. to estimate the risk of campylobacter infections in duck farm workers, we investigated the prevalence of campylobacter spp. in ducks of two duck farms and the seroprevalence of anti-campylobacter antibodies (iga and igg) in two cohorts of workers. the first cohort consisted of high-exposed stable workers ... | 2016 | 27429794 |
| complete genome sequences of campylobacter hyointestinalis subsp. hyointestinalis strain lmg 9260 and c. hyointestinalis subsp. lawsonii strain lmg 15993. | campylobacter hyointestinalis is isolated primarily from ruminants and swine, but is also occasionally isolated from humans. c. hyointestinalis is currently divided into two subspecies, c. hyointestinalis subsp. hyointestinalis and c. hyointestinalis subsp. lawsonii this study describes the first closed whole-genome sequences of c. hyointestinalis subsp. hyointestinalis isolate lmg 9260 and c. hyointestinalis subsp. lawsonii isolate lmg 15993. | 2016 | 27417840 |
| novel immunomodulatory flagellin-like protein flac in campylobacter jejuni and other campylobacterales. | the human diarrheal pathogens campylobacter jejuni and campylobacter coli interfere with host innate immune signaling by different means, and their flagellins, flaa and flab, have a low intrinsic property to activate the innate immune receptor toll-like receptor 5 (tlr5). we have investigated here the hypothesis that the unusual secreted, flagellin-like molecule flac present in c. jejuni, c. coli, and other campylobacterales might activate cells via tlr5 and interact with tlr5. flac shows striki ... | 2015 | 27303676 |
| novel immunomodulatory flagellin-like protein flac in campylobacter jejuni and other campylobacterales. | the human diarrheal pathogens campylobacter jejuni and campylobacter coli interfere with host innate immune signaling by different means, and their flagellins, flaa and flab, have a low intrinsic property to activate the innate immune receptor toll-like receptor 5 (tlr5). we have investigated here the hypothesis that the unusual secreted, flagellin-like molecule flac present in c. jejuni, c. coli, and other campylobacterales might activate cells via tlr5 and interact with tlr5. flac shows striki ... | 2015 | 27303676 |
| optimized cultivation of campylobacter concisus from gut mucosal biopsies in inflammatory bowel disease. | campylobacter concisus is a commensal of the human oral flora that has been linked to prolonged diarrhea and inflammatory bowel disease (ibd). it has been detected more often from intestinal biopsies in patients with ibd compared to healthy controls using pcr-based techniques, whereas the number of c. concisus culture-positive biopsies in previous studies has been very limited. determining the rate of viable isolates present in the gut mucosa is of great importance when evaluating the role in di ... | 2016 | 27252786 |
| evaluation of various campylobacter-specific quantitative pcr (qpcr) assays for detection and enumeration of campylobacteraceae in irrigation water and wastewater via a miniaturized most-probable-number-qpcr assay. | campylobacter spp. are the leading cause of bacterial gastroenteritis worldwide, and water is increasingly seen as a risk factor in transmission. here we describe a most-probable-number (mpn)-quantitative pcr (qpcr) assay in which water samples are centrifuged and aliquoted into microtiter plates and the bacteria are enumerated by qpcr. we observed that commonly used campylobacter molecular assays produced vastly different detection rates. in irrigation water samples, detection rates varied depe ... | 2016 | 27235434 |
| treatment and prevention of infection following bites of the antarctic fur seal (arctocephalus gazella). | in recent decades, an increasing number of people have traveled to sub-antarctic and antarctic regions each year for research, tourism, and resource exploitation. hunting of antarctic fur seals (arctocephalus gazella) almost pushed the species to extinction in the early 1900s, but populations have since shown rapid and substantial recovery. the species' range has re-expanded to include several islands south of the antarctic convergence, most notably south georgia, and now overlaps with many popu ... | 2015 | 27147885 |
| molecular identification of campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being. | campylobacter is an emerging zoonotic pathogen and one of the leading cause of foodborne infection worldwide and it has been isolated from a variety of animal species. the aim of this study was to identify campylobacter jejuni and campylobacter coli from dogs, calves, and poultry using polymerase chain reaction (pcr). | 2015 | 27047055 |
| subtyping of salmonella enterica subspecies i using single-nucleotide polymorphisms in adenylate cyclase. | methods to rapidly identify serotypes of salmonella enterica subspecies i are of vital importance for protecting the safety of food. to supplement the serotyping method dkgb-linked intergenic sequence ribotyping (isr), single-nucleotide polymorphisms were characterized within adenylate cyclase (cyaa). the national center for biotechnology information (ncbi) database had 378 cyaa sequences from s. enterica subspecies i, which included 42 unique dna sequences and 19 different amino acid sequences. ... | 2016 | 27035032 |
| temporal variation in the prevalence and species richness of campylobacter spp. in a prairie watershed impacted by urban and agricultural mixed inputs. | campylobacter spp. are a substantial cause of gastroenteritis worldwide. human infection can result from ingestion of contaminated food or water from a variety of sources, including the consumption of fresh produce that is contaminated with the pathogen via the use of contaminated irrigation water. using molecular methods, we investigated the occurrence of campylobacter in the qu'appelle river watershed, an important source of irrigation water for vegetable producers in southern saskatchewan, ca ... | 2016 | 27003220 |
| probing polytopic membrane protein-substrate interactions by luminescence resonance energy transfer. | integral membrane proteins play essential roles in all living systems; however, major technical hurdles challenge analyses of this class of proteins. biophysical approaches that provide structural information to complement and leverage experimentally determined and computationally predicted structures are urgently needed. herein we present the application of luminescence resonance energy transfer (lret) for investigating the interactions of the polytopic membrane-bound oligosaccharyl transferase ... | 2016 | 26918528 |
| revisiting the roles of culture and culture-independent detection tests for campylobacter. | culture-independent detection tests (cidts) for campylobacter have become an area of intense controversy and confusion among laboratorians in the field of clinical microbiology. to date, the true analytical and clinical performance of stool antigen cidts versus truly optimized culture conditions is unknown. in this issue of the journal of clinical microbiology, fitzgerald and colleagues (c. fitzgerald et al., j clin microbiol 54:1209-1215, 2016, http://dx.doi.org/10.1128/jcm.01925-15) report com ... | 2016 | 26912756 |
| structures of aminoarabinose transferase arnt suggest a molecular basis for lipid a glycosylation. | polymyxins are antibiotics used in the last line of defense to combat multidrug-resistant infections by gram-negative bacteria. polymyxin resistance arises through charge modification of the bacterial outer membrane with the attachment of the cationic sugar 4-amino-4-deoxy-l-arabinose to lipid a, a reaction catalyzed by the integral membrane lipid-to-lipid glycosyltransferase 4-amino-4-deoxy-l-arabinose transferase (arnt). here, we report crystal structures of arnt from cupriavidus metallidurans ... | 2016 | 26912703 |
| smrt sequencing of the campylobacter coli bfr-ca-9557 genome sequence reveals unique methylation motifs. | campylobacter species are the most prevalent bacterial pathogen causing acute enteritis worldwide. in contrast to campylobacter jejuni, about 5 % of campylobacter coli strains exhibit susceptibility to restriction endonuclease digestion by dpni cutting specifically 5'-g(m)atc-3' motifs. this indicates significant differences in dna methylation between both microbial species. the goal of the study was to analyze the methylome of a c. coli strain susceptible to dpni digestion, to identify its meth ... | 2015 | 26689587 |
| functional analysis of n-linking oligosaccharyl transferase enzymes encoded by deep-sea vent proteobacteria. | bacterial n-linking oligosaccharyl transferases (otase enzymes) transfer lipid-linked glycans to selected proteins in the periplasm and were first described in the intestinal pathogen campylobacter jejuni, a member of the ε-proteobacteria-subdivision of bacteria. more recently, orthologues from other ε-proteobacterial campylobacter and helicobacter species and a δ-proteobacterium, desulfovibrio desulfuricans, have been described, suggesting that these two subdivisions of bacteria may be a source ... | 2015 | 26610891 |
| functional analysis of n-linking oligosaccharyl transferase enzymes encoded by deep-sea vent proteobacteria. | bacterial n-linking oligosaccharyl transferases (otase enzymes) transfer lipid-linked glycans to selected proteins in the periplasm and were first described in the intestinal pathogen campylobacter jejuni, a member of the ε-proteobacteria-subdivision of bacteria. more recently, orthologues from other ε-proteobacterial campylobacter and helicobacter species and a δ-proteobacterium, desulfovibrio desulfuricans, have been described, suggesting that these two subdivisions of bacteria may be a source ... | 2015 | 26610891 |
| arnt proteins that catalyze the glycosylation of lipopolysaccharide share common features with bacterial n-oligosaccharyltransferases. | arnt is a glycosyltransferase that catalyzes the addition of 4-amino-4-deoxy-l-arabinose (l-ara4n) to the lipid a moiety of the lipopolysaccharide. this is a critical modification enabling bacteria to resist killing by antimicrobial peptides. arnt is an integral inner membrane protein consisting of 13 predicted transmembrane helices and a large periplasmic c-terminal domain. we report here the identification of a functional motif with a canonical consensus sequence dexryax(5)mx(3)gxwx(9)yfekpx(4 ... | 2016 | 26515403 |
| milk modulates campylobacter invasion into caco-2 intestinal epithelial cells. | raw milk is a recognized source of campylobacter outbreaks, but pasteurization is an effective way to eliminate the causative agent of campylobacteriosis. whereas breastfeeding is protective against infectious diseases, consumption of formula milk is thought to be not. however, in relation to campylobacter, such data is currently unavailable. although both pasteurized and formula milk are pathogen free and prepared in a quality controlled manner, the effect they have on the virulence of campylob ... | 2015 | 26495128 |
| substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique n-glycosylation site preferences. | the central enzyme in the campylobacter jejuni asparagine-linked glycosylation pathway is the oligosaccharyltransferase (ost), pglb, which transfers preassembled glycans to specific asparagine residues in target proteins. while c. jejuni pglb (cjpglb) can transfer many diverse glycan structures, the acceptor sites that it recognizes are restricted predominantly to those having a negatively charged residue in the -2 position relative to the asparagine. here, we investigated the acceptor-site pref ... | 2015 | 26482295 |
| genome sequence of a urease-positive campylobacter lari strain. | campylobacter lari is frequently isolated from shore birds and can cause illness in humans. here, we report the draft whole-genome sequence of a urease-positive strain of c. lari that was isolated in estuarial water on the coast of delaware, usa. | 2015 | 26472835 |
| campylobacter species in animal, food, and environmental sources, and relevant testing programs in canada. | campylobacter species, particularly thermophilic campylobacters, have emerged as a leading cause of human foodborne gastroenteritis worldwide, with campylobacter jejuni, campylobacter coli, and campylobacter lari responsible for the majority of human infections. although most cases of campylobacteriosis are self-limiting, campylobacteriosis represents a significant public health burden. human illness caused by infection with campylobacters has been reported across canada since the early 1970s. m ... | 2015 | 26422448 |
| evolution of the selenoproteome in helicobacter pylori and epsilonproteobacteria. | by competing for the acquisition of essential nutrients, helicobacter pylori has the unique ability to persist in the human stomach, also causing nutritional insufficiencies in the host. although the h. pylori genome apparently encodes selenocysteine synthase (sela, hp1513), a key pyridoxal phosphate (plp)-dependent enzyme for the incorporation of selenium into bacterial proteins, nothing is known about the use of this essential element in protein synthesis by this pathogen. we analyzed the evol ... | 2015 | 26342139 |
| differential distribution of type ii crispr-cas systems in agricultural and nonagricultural campylobacter coli and campylobacter jejuni isolates correlates with lack of shared environments. | crispr (clustered regularly interspaced palindromic repeats)-cas (crispr-associated) systems are sequence-specific adaptive defenses against phages and plasmids which are widespread in prokaryotes. here we have studied whether phylogenetic relatedness or sharing of environmental niches affects the distribution and dissemination of type ii crispr-cas systems, first in 132 bacterial genomes from 15 phylogenetic classes, ranging from proteobacteria to actinobacteria. there was clustering of distinc ... | 2015 | 26338188 |
| survey of clustered regularly interspaced short palindromic repeats and their associated cas proteins (crispr/cas) systems in multiple sequenced strains of klebsiella pneumoniae. | in recent years the emergence of multidrug resistant klebsiella pneumoniae strains has been an increasingly common event. this opportunistic species is one of the five main bacterial pathogens that cause hospital infections worldwide and multidrug resistance has been associated with the presence of high molecular weight plasmids. plasmids are generally acquired through horizontal transfer and therefore is possible that systems that prevent the entry of foreign genetic material are inactive or ab ... | 2015 | 26238567 |
| rapid detection of campylobacter jejuni, campylobacter coli, and campylobacter lari in fresh chicken meat and by-products in bangkok, thailand, using modified multiplex pcr. | a multiplex pcr assay for simultaneous detection and differentiation of campylobacter jejuni, campylobacter coli, and campylobacter lari was developed and validated to assess the occurrence of these bacteria in fresh chicken meat and by-products in bangkok, thailand, by using a new combination of four previously published pcr primers for c. jejuni, c. coli, c. lari, and a universal 16s rdna gene as an internal control. the specificity was determined by using 13 strains of other bacteria. with pu ... | 2015 | 26197289 |
| effects of nitrate addition on rumen fermentation, bacterial biodiversity and abundance. | this study examined changes of rumen fermentation, ruminal bacteria biodiversity and abundance caused by nitrate addition with ion torrent sequencing and real-time polymerase chain reaction. three rumen-fistulated steers were fed diets supplemented with 0%, 1%, and 2% nitrate (dry matter %) in succession. nitrate supplementation linearly increased total volatile fatty acids and acetate concentration obviously (p = 0.02; p = 0.02; p<0.01), butyrate and isovalerate concentration numerically (p = 0 ... | 2015 | 26194220 |
| transformation and characterization of an arsenic gene operon from urease-positive thermophilic campylobacter (uptc) in escherichia coli. | an arsenate susceptibility test was performed with transformed and cultured escherichia coli dh5α cells, which carried recombinant dna of full-length arsenic (ars) operon, namely a putative membrane permease, arsp; a transcriptional repressor, arsr; an arsenate reductase, arsc; and an arsenical-resistance membrane transporter, acr3, from the japanese urease-positive thermophilic campylobacter lari (uptc) cf89-12. the e. coli dh5α transformant showed reduced susceptibility to arsenate (~1536 μg/m ... | 2016 | 26122364 |
| global epidemiology of campylobacter infection. | campylobacter jejuni infection is one of the most widespread infectious diseases of the last century. the incidence and prevalence of campylobacteriosis have increased in both developed and developing countries over the last 10 years. the dramatic increase in north america, europe, and australia is alarming, and data from parts of africa, asia, and the middle east indicate that campylobacteriosis is endemic in these areas, especially in children. in addition to c. jejuni, there is increasing rec ... | 2015 | 26062576 |
| molecular analysis of the tlya gene in campylobacter lari. | full-length tlya gene and its adjacent genetic loci from the urease-positive thermophilic campylobacter (uptc) cf89-12 [approximately 15,000 base pairs (bp) in length], as well as a reference strain campylobacter lari rm2100 (approximately 9,000 bp), were analyzed. the possible open-reading frame of tlya from uptc cf89-12 was shown to have 720 bp with a calculated molecular mass of approximately 26.7 kda. using a primer pair designed in silico, a total of approximately 1.1 kbp consisting of puta ... | 2015 | 25906999 |
| increased efficiency of campylobacter jejuni n-oligosaccharyltransferase pglb by structure-guided engineering. | conjugate vaccines belong to the most efficient preventive measures against life-threatening bacterial infections. functional expression of n-oligosaccharyltransferase (n-ost) pglb of campylobacter jejuni in escherichia coli enables a simplified production of glycoconjugate vaccines in prokaryotic cells. polysaccharide antigens of pathogenic bacteria can be covalently coupled to immunogenic acceptor proteins bearing engineered glycosylation sites. transfer efficiency of pglbcj is low for certain ... | 2015 | 25833378 |
| first case report of campylobacter volucris bacteremia in an immunocompromised patient. | we report a case of campylobacter volucris bacteremia in an immunocompromised patient with polycythemia vera and alcoholic liver cirrhosis. to our knowledge, this is the first case report in which this organism has been isolated from a human clinical specimen. | 2015 | 25832303 |
| detection of thermophilic campylobacter sp. in raw chicken sausages by methods iso 10272: 2006 in curitiba - parana state - brazil. | the aim of this study was the detection of campylobacter sp. in raw chicken sausages using the methods iso 10272-1 and iso 10272-2. the overall prevalence of campylobacter sp. in the samples tested was 16.67%, representing a serious risk to the health of consumers, particularly if measures guaranteeing proper cooking of foods and prevention of cross-contamination are not adopted. furthermore, the majority of campylobacteriosis cases in humans are caused by consumption or improper handling of con ... | 2015 | 25763066 |
| detection of thermophilic campylobacter sp. in raw chicken sausages by methods iso 10272: 2006 in curitiba - parana state - brazil. | the aim of this study was the detection of campylobacter sp. in raw chicken sausages using the methods iso 10272-1 and iso 10272-2. the overall prevalence of campylobacter sp. in the samples tested was 16.67%, representing a serious risk to the health of consumers, particularly if measures guaranteeing proper cooking of foods and prevention of cross-contamination are not adopted. furthermore, the majority of campylobacteriosis cases in humans are caused by consumption or improper handling of con ... | 2015 | 25763066 |
| life history correlates of fecal bacterial species richness in a wild population of the blue tit cyanistes caeruleus. | very little is known about the normal gastrointestinal flora of wild birds, or how it might affect or reflect the host's life-history traits. the aim of this study was to survey the species richness of bacteria in the feces of a wild population of blue tits cyanistes caeruleus and to explore the relationships between bacterial species richness and various life-history traits, such as age, sex, and reproductive success. using pcr-tgge, 55 operational taxonomic units (otus) were identified in blue ... | 2015 | 25750710 |
| genome-wide gene order distances support clustering the gram-positive bacteria. | initially using 143 genomes, we developed a method for calculating the pair-wise distance between prokaryotic genomes using a monte carlo method to estimate the conservation of gene order. the method was based on repeatedly selecting five or six non-adjacent random orthologs from each of two genomes and determining if the chosen orthologs were in the same order. the raw distances were then corrected for gene order convergence using an adaptation of the jukes-cantor model, as well as using the co ... | 2015 | 25653643 |
| genome-wide gene order distances support clustering the gram-positive bacteria. | initially using 143 genomes, we developed a method for calculating the pair-wise distance between prokaryotic genomes using a monte carlo method to estimate the conservation of gene order. the method was based on repeatedly selecting five or six non-adjacent random orthologs from each of two genomes and determining if the chosen orthologs were in the same order. the raw distances were then corrected for gene order convergence using an adaptation of the jukes-cantor model, as well as using the co ... | 2015 | 25653643 |
| in vivo production of a novel glycoconjugate vaccine against shigella flexneri 2a in recombinant escherichia coli: identification of stimulating factors for in vivo glycosylation. | glycoconjugated vaccines composed of polysaccharide antigens covalently linked to immunogenic carrier proteins have proved to belong to the most effective and safest vaccines for combating bacterial pathogens. the functional transfer of the n-glycosylation machinery from campylobacter jejuni to the standard prokaryotic host escherichia coli established a novel bioconjugation methodology termed bacterial glycoengineering. | 2015 | 25612741 |
| detection of mycobacteria, mycobacterium avium subspecies, and mycobacterium tuberculosis complex by a novel tetraplex real-time pcr assay. | mycobacterium tuberculosis complex, mycobacterium avium, and many other nontuberculous mycobacteria are worldwide distributed microorganisms of major medical and veterinary importance. considering the growing epidemiologic significance of wildlife-livestock-human interrelation, developing rapid detection tools of high specificity and sensitivity is vital to assess their presence and accelerate the process of diagnosing mycobacteriosis. here we describe the development and evaluation of a novel t ... | 2015 | 25588660 |
| laboratory diagnosis of bacterial gastroenteritis. | bacterial gastroenteritis is a disease that is pervasive in both the developing and developed worlds. while for the most part bacterial gastroenteritis is self-limiting, identification of an etiological agent by bacterial stool culture is required for the management of patients with severe or prolonged diarrhea, symptoms consistent with invasive disease, or a history that may predict a complicated course of disease. importantly, characterization of bacterial enteropathogens from stool cultures i ... | 2015 | 25567220 |
| first and stereoselective synthesis of an α-(2→5)-linked disaccharide of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo). | resistance of bacterial pathogens toward antibiotics has revived interest in lipopolysaccharide (lps) motifs as potential therapeutic targets. the lps of several pathogenic acinetobacter strains comprises a 4,5-branched kdo trisaccharide containing an uncommon (2→5)-linkage. in this contribution the first stereoselective glycosylation method for obtaining an α-kdo-(2→5)-α-kdo disaccharide in good yield is highlighted. the synthetic approach used for accessing this linkage type will allow for fut ... | 2014 | 25496419 |
| first and stereoselective synthesis of an α-(2→5)-linked disaccharide of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo). | resistance of bacterial pathogens toward antibiotics has revived interest in lipopolysaccharide (lps) motifs as potential therapeutic targets. the lps of several pathogenic acinetobacter strains comprises a 4,5-branched kdo trisaccharide containing an uncommon (2→5)-linkage. in this contribution the first stereoselective glycosylation method for obtaining an α-kdo-(2→5)-α-kdo disaccharide in good yield is highlighted. the synthetic approach used for accessing this linkage type will allow for fut ... | 2014 | 25496419 |
| lipid-linked oligosaccharides in membranes sample conformations that facilitate binding to oligosaccharyltransferase. | lipid-linked oligosaccharides (llos) are the substrates of oligosaccharyltransferase (ost), the enzyme that catalyzes the en bloc transfer of the oligosaccharide onto the acceptor asparagine of nascent proteins during the process of n-glycosylation. to explore llos' preferred location, orientation, structure, and dynamics in membrane bilayers of three different lipid types (dilauroylphosphatidylcholine, dimyristoylphosphatidylcholine, and dioleoylphosphatidylcholine), we have modeled and simulat ... | 2014 | 25418169 |
| comparative genomics of the campylobacter lari group. | the campylobacter lari group is a phylogenetic clade within the epsilon subdivision of the proteobacteria and is part of the thermotolerant campylobacter spp., a division within the genus that includes the human pathogen campylobacter jejuni. the c. lari group is currently composed of five species (c. lari, campylobacter insulaenigrae, campylobacter volucris, campylobacter subantarcticus, and campylobacter peloridis), as well as a group of strains termed the urease-positive thermophilic campylob ... | 2014 | 25381664 |
| prevalence of salmonella and campylobacter on broiler chickens from farm to slaughter and efficiency of methods to remove visible fecal contamination. | a study was conducted to investigate the prevalence of salmonella and campylobacter from farm to slaughter. the efficiency of trimming and water spray (490 to 588 kpa pressure) on the removal of visible fecal contamination from broiler carcasses before chilling was also investigated. drag swabs were used to sample litter from the farm houses. samples of ceca and carcasses without and with visible fecal contamination before and after trimming or spray washing of fecal contamination were taken dur ... | 2014 | 25364917 |
| metaphylogenomic and potential functionality of the limpet patella pellucida's gastrointestinal tract microbiome. | this study investigated the microbial diversity associated with the digestive tract of the seaweed grazing marine limpet patella pellucida. using a modified indirect dna extraction protocol and performing metagenomic profiling based on specific prokaryotic marker genes, the abundance of bacterial groups was identified from the analyzed metagenome. the members of three significantly abundant phyla of proteobacteria, firmicutes and bacteroidetes were characterized through the literature and their ... | 2014 | 25334059 |
| hijacking bacterial glycosylation for the production of glycoconjugates, from vaccines to humanised glycoproteins. | glycosylation or the modification of a cellular component with a carbohydrate moiety has been demonstrated in all three domains of life as a basic post-translational process important in a range of biological processes. this review will focus on the latest studies attempting to exploit bacterial n-linked protein glycosylation for glycobiotechnological applications including glycoconjugate vaccine and humanised glycoprotein production. the challenges that remain for these approaches to reach full ... | 2014 | 25244672 |
| hijacking bacterial glycosylation for the production of glycoconjugates, from vaccines to humanised glycoproteins. | glycosylation or the modification of a cellular component with a carbohydrate moiety has been demonstrated in all three domains of life as a basic post-translational process important in a range of biological processes. this review will focus on the latest studies attempting to exploit bacterial n-linked protein glycosylation for glycobiotechnological applications including glycoconjugate vaccine and humanised glycoprotein production. the challenges that remain for these approaches to reach full ... | 2014 | 25244672 |
| advances in understanding glycosyltransferases from a structural perspective. | glycosyltransferases (gts), the enzymes that catalyse glycosidic bond formation, create a diverse range of saccharides and glycoconjugates in nature. understanding gts at the molecular level, through structural and kinetic studies, is important for gaining insights into their function. in addition, this understanding can help identify those enzymes which are involved in diseases, or that could be engineered to synthesize biologically or medically relevant molecules. this review describes how str ... | 2014 | 25240227 |
| accurate detection of campylobacter spp. antigens by immunochromatography and enzyme immunoassay in routine microbiological laboratory. | campylobacter spp. are fastidious microorganisms, and their detection by culture depends on the freshness of the stool sample and the skills of the laboratory staff. to improve laboratory diagnosis, assays for the detection of specific antigens have been developed. here, we evaluated two assays for the detection of campylobacter spp.-specific antigens, i.e., one immunochromatographic test and one enzyme-linked immunosorbent assay (eia), in 38 frozen campylobacter spp.-positive specimens and pros ... | 2014 | 25215191 |
| genomic evidence for the emergence and evolution of pathogenicity and niche preferences in the genus campylobacter. | the genus campylobacter includes some of the most relevant pathogens for human and animal health; the continuous effort in their characterization has also revealed new species putatively involved in different kind of infections. nowadays, the available genomic data for the genus comprise a wide variety of species with different pathogenic potential and niche preferences. in this work, we contribute to enlarge this available information presenting the first genome for the species campylobacter sp ... | 2014 | 25193310 |
| detection, identification and quantification of campylobacter jejuni, coli and lari in food matrices all at once using multiplex qpcr. | thermotolerant campylobacter jejuni, coli and lari are recognized as leading food-borne pathogens causing an acute bacterial enteritis worldwide. due to narrow spectrum of their biochemical activity, it is very complicated to distinguish between individual species. for reliable risk assessment, proper incidence evaluation or swift sample analysis regarding individual species, a demand for simple and rapid method for their distinguishing is reasonable. in this study, we evaluated a reliable and s ... | 2014 | 25057300 |
| campylobacter lari in a platelet donation: an unexpected finding. | 2014 | 25041073 | |
| the role of intracellular organisms in the pathogenesis of inflammatory arthritis. | inflammatory arthritis is a condition which is characterised by recurrent episodes of joint pain and swelling. it encompasses a spectrum of disorders ranging from rheumatoid arthritis to ankylosing spondylitis. in these conditions, for reasons that are poorly understood, the immune system raises an inflammatory response within the joint space. in some cases, autoantigens have been identified (e.g., anticitrullinated peptides in rheumatoid arthritis), but the absence of these, in the seronegative ... | 2014 | 24995143 |
| same-day subtyping of campylobacter jejuni and c. coli isolates by use of multiplex ligation-dependent probe amplification-binary typing. | campylobacteriosis is the most commonly reported form of human bacterial gastroenteritis in the world. sound identification of infectious sources requires subtyping, but the most widely used methods have turnaround times measured in days and require specialist equipment and skills. a multiplex ligation-dependent probe amplification-binary typing (mbit) assay was developed for subtyping campylobacter jejuni and campylobacter coli. it was tested on 245 isolates, including recent isolates from belg ... | 2014 | 24989612 |
| substrate specificity of cytoplasmic n-glycosyltransferase. | n-linked protein glycosylation is a very common post-translational modification that can be found in all kingdoms of life. the classical, highly conserved pathway entails the assembly of a lipid-linked oligosaccharide and its transfer to an asparagine residue in the sequon nx(s/t) of a secreted protein by the integral membrane protein oligosaccharyltransferase. a few species in the class of γ-proteobacteria encode a cytoplasmic n-glycosylation system mediated by a soluble n-glycosyltransferase ( ... | 2014 | 24962585 |
| a national investigation of the prevalence and diversity of thermophilic campylobacter species in agricultural watersheds in canada. | the occurrence and diversity of thermophilic campylobacter species (c. jejuni, coli, and lari) were studied in water samples from four river basins located across canada. these basins located in quebec (bras d'henri), alberta (oldman), ontario (south nation), and british columbia (sumas) represented some of the most intensive farming areas in canada for hog, beef cattle, dairy cattle, and poultry, respectively. this study analyzed 769 water samples collected from 23 monitoring sites with agricul ... | 2014 | 24930011 |
| the importance of the viable but non-culturable state in human bacterial pathogens. | many bacterial species have been found to exist in a viable but non-culturable (vbnc) state since its discovery in 1982. vbnc cells are characterized by a loss of culturability on routine agar, which impairs their detection by conventional plate count techniques. this leads to an underestimation of total viable cells in environmental or clinical samples, and thus poses a risk to public health. in this review, we present recent findings on the vbnc state of human bacterial pathogens. the characte ... | 2014 | 24917854 |
| aglb, catalyzing the oligosaccharyl transferase step of the archaeal n-glycosylation process, is essential in the thermoacidophilic crenarchaeon sulfolobus acidocaldarius. | sulfolobus acidocaldarius, a thermo-acidophilic crenarchaeon which grows optimally at 76 °c and ph 3, exhibits an astonishing high number of n-glycans linked to the surface (s-) layer proteins. the s-layer proteins as well as other surface-exposed proteins are modified via n-glycosylation, in which the oligosaccharyl transferase aglb catalyzes the final step of the transfer of the glycan tree to the nascent protein. in this study, we demonstrated that aglb is essential for the viability of s. ac ... | 2014 | 24916761 |
| n-linked glycosylation in archaea: a structural, functional, and genetic analysis. | n-glycosylation of proteins is one of the most prevalent posttranslational modifications in nature. accordingly, a pathway with shared commonalities is found in all three domains of life. while excellent model systems have been developed for studying n-glycosylation in both eukarya and bacteria, an understanding of this process in archaea was hampered until recently by a lack of effective molecular tools. however, within the last decade, impressive advances in the study of the archaeal version o ... | 2014 | 24847024 |
| annotation of protein domains reveals remarkable conservation in the functional make up of proteomes across superkingdoms. | the functional repertoire of a cell is largely embodied in its proteome, the collection of proteins encoded in the genome of an organism. the molecular functions of proteins are the direct consequence of their structure and structure can be inferred from sequence using hidden markov models of structural recognition. here we analyze the functional annotation of protein domain structures in almost a thousand sequenced genomes, exploring the functional and structural diversity of proteomes. we find ... | 2011 | 24710297 |
| a multiplex pcr/ldr assay for simultaneous detection and identification of the niaid category b bacterial food and water-borne pathogens. | enteric pathogens that cause gastroenteritis remain a major global health concern. the goal of this study was to develop a multiplex pcr/ligation detection reaction (ldr) assay for the detection of all niaid category b bacterial food and water-borne pathogens directly from stool specimens. to validate the pcr/ldr assay, clinical isolates of campylobacter spp., vibrio spp., shigella spp., salmonella spp., listeria monocytogenes, yersinia enterocolitica, and diarrheagenic escherichia coli were tes ... | 2014 | 24709368 |
| molecular structural analysis of major outer membrane protein (momp) gene clusters in campylobacter lari. | southern hybridisation shows that urease-negative (un) campylobacter lari jcm2530(t) carries two putative major outer membrane protein (momp) genes. sequences of approximately 2.1 kbp, encoding non-coding (nc) regions, with possible open reading frames (orfs) for momp (pora1 or pora2) of approximately 1.2 kbp, nc regions and partial and putative cla_0435 or cla_1109 orfs were identified in all five un c. lari isolates examined, following polymerase chain reaction (pcr) cloning and sequencing. ea ... | 2014 | 24693571 |
| validation according to iso 16140:2003 of a commercial real-time pcr-based method for detecting campylobacter jejuni, c. coli, and c. lari in foods. | campylobacteriosis was the most frequently reported zoonosis in the european union (eu) in 2010, with campylobacter jejuni, campylobacter coli, and campylobacter lari as the most frequently reported species in foodborne outbreaks (fbos). relatively sensitive to environmental factors, these species may be present in low numbers. in line with eu policy for food control and fbo detection and in view of the need to reduce response time, we validated an alternative molecular method according to iso 1 ... | 2014 | 24607425 |
| divergent distribution of the sensor kinase coss in non-thermotolerant campylobacter species and its functional incompatibility with the response regulator cosr of campylobacter jejuni. | two-component signal transduction systems are commonly composed of a sensor histidine kinase and a cognate response regulator, modulating gene expression in response to environmental changes through a phosphorylation-dependent process. cosr is an ompr-type response regulator essential for the viability of campylobacter jejuni, a major foodborne pathogenic species causing human gastroenteritis. although cosr is a response regulator, its cognate sensor kinase has not been identified in c. jejuni. ... | 2014 | 24587027 |
| campylobacter prosthetic joint infection. | a 75-year-old man was diagnosed with probable campylobacter jejuni prosthetic knee infection after a diarrheal illness. joint aspirate and operative cultures were negative, but pcr of prosthesis sonicate fluid was positive, as was stool culture. nineteen additional cases of campylobacter prosthetic joint infection reported in the literature are reviewed. | 2014 | 24523462 |
| evaluation of a loop-mediated isothermal amplification suite for the rapid, reliable, and robust detection of shiga toxin-producing escherichia coli in produce. | shiga toxin-producing escherichia coli (stec) strains are a leading cause of produce-associated outbreaks in the united states. rapid, reliable, and robust detection methods are needed to better ensure produce safety. we recently developed a loop-mediated isothermal amplification (lamp) suite for stec detection. in this study, the stec lamp suite was comprehensively evaluated against real-time quantitative pcr (qpcr) using a large panel of bacterial strains (n = 156) and various produce items (s ... | 2014 | 24509927 |
| biological roles of the o-methyl phosphoramidate capsule modification in campylobacter jejuni. | campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide, and the capsular polysaccharide (cps) of this organism is required for persistence and disease. c. jejuni produces over 47 different capsular structures, including a unique o-methyl phosphoramidate (meopn) modification present on most c. jejuni isolates. although the meopn structure is rare in nature it has structural similarity to some synthetic pesticides. in this study, we have demonstrated, by whole genome comparis ... | 2014 | 24498018 |
| comparative analysis and limitations of ethidium monoazide and propidium monoazide treatments for the differentiation of viable and nonviable campylobacter cells. | the lack of differentiation between viable and nonviable bacterial cells limits the implementation of pcr-based methods for routine diagnostic approaches. recently, the combination of a quantitative real-time pcr (qpcr) and ethidium monoazide (ema) or propidium monoazide (pma) pretreatment has been described to circumvent this disadvantage. in regard to the suitability of this approach for campylobacter spp., conflicting results have been reported. thus, we compared the suitabilities of ema and ... | 2014 | 24487529 |
| presence and analysis of plasmids in human and animal associated arcobacter species. | in this study, we report the screening of four arcobacter species for the presence of small and large plasmids. plasmids were present in 9.9% of the 273 examined strains. one arcobacter cryaerophilus and four arcobacter butzleri plasmids were selected for further sequencing. the size of three small plasmids isolated from a. butzleri and the one from a. cryaerophilus strains ranged between 4.8 and 5.1 kb, and the size of the large plasmid, isolated from a. butzleri, was 27.4 kbp. the g+c content ... | 2014 | 24465575 |
| deep-sea hydrothermal vent epsilonproteobacteria encode a conserved and widespread nitrate reduction pathway (nap). | despite the frequent isolation of nitrate-respiring epsilonproteobacteria from deep-sea hydrothermal vents, the genes coding for the nitrate reduction pathway in these organisms have not been investigated in depth. in this study we have shown that the gene cluster coding for the periplasmic nitrate reductase complex (nap) is highly conserved in chemolithoautotrophic, nitrate-reducing epsilonproteobacteria from deep-sea hydrothermal vents. furthermore, we have shown that the napa gene is expresse ... | 2014 | 24430487 |
| identification and characterization of an invasion antigen b gene from the oral pathogen campylobacter rectus. | the oral bacterium, campylobacter rectus, is an etiological agent of periodontitis. the virulence genes of c. rectus are largely unknown. the aim of this study was to query c. rectus for the presence of an invasion antigen b (ciab) gene, which is needed for cell invasion by the related species campylobacter jejuni. pcr and pcr-walking identified a ciab from c. rectus. in silico analyses of c. rectus 314 ciab (cr-ciab) revealed an orf of 1,830 base pairs. the cr-ciab protein shared significant se ... | 2014 | 24426164 |
| identification and characterization of an invasion antigen b gene from the oral pathogen campylobacter rectus. | the oral bacterium, campylobacter rectus, is an etiological agent of periodontitis. the virulence genes of c. rectus are largely unknown. the aim of this study was to query c. rectus for the presence of an invasion antigen b (ciab) gene, which is needed for cell invasion by the related species campylobacter jejuni. pcr and pcr-walking identified a ciab from c. rectus. in silico analyses of c. rectus 314 ciab (cr-ciab) revealed an orf of 1,830 base pairs. the cr-ciab protein shared significant se ... | 2014 | 24426164 |
| intestinal microbiota and species diversity of campylobacter and helicobacter spp. in migrating shorebirds in delaware bay. | using 16s rrna gene sequencing analysis, we examined the bacterial diversity and the presence of opportunistic bacterial pathogens (i.e., campylobacter and helicobacter) in red knot (calidris canutus; n = 40), ruddy turnstone (arenaria interpres; n = 35), and semipalmated sandpiper (calidris pusilla; n = 22) fecal samples collected during a migratory stopover in delaware bay. additionally, we studied the occurrence of campylobacter spp., enterococci, and waterfowl fecal source markers using quan ... | 2014 | 24413599 |
| molecular cloning and characterisation of the methionine sulphoxide reductase a (msra) gene locus in campylobacter lari organisms. | the methionine sulphoxide reductase a (msra) gene and its adjacent genetic loci from urease-negative (un) campylobacter lari rm2100 and urease-positive thermophilic campylobacter (uptc)cf89-12 strains appear to be composed of a msra structure gene (507 base pairs [bp]) and another five-gene cluster (approximately 6300 bp) in the same strand and direction. a primer pair (f1/r4-msra) for polymerase chain reaction (pcr) amplification was designed to generate a product of approximately 900 bp of the ... | 2013 | 24400424 |
| prevalence and pathogenic potential of campylobacter isolates from free-living, human-commensal american crows. | recent studies have suggested a potential role for wild birds in zoonotic transmission of campylobacter jejuni, the leading cause of gastroenteritis in humans worldwide. in this study, we detected campylobacter spp. in 66.9% (85/127) of free-ranging american crows (corvus brachyrhyncos) sampled in the sacramento valley of california in 2012 and 2013. biochemical testing and sequence analysis of 16s rrna revealed that 93% of isolates (n = 70) were c. jejuni, with cytolethal distending toxin (cdt) ... | 2014 | 24375131 |
| the complex methylome of the human gastric pathogen helicobacter pylori. | the genome of helicobacter pylori is remarkable for its large number of restriction-modification (r-m) systems, and strain-specific diversity in r-m systems has been suggested to limit natural transformation, the major driving force of genetic diversification in h. pylori. we have determined the comprehensive methylomes of two h. pylori strains at single base resolution, using single molecule real-time (smrt®) sequencing. for strains 26695 and j99-r3, 17 and 22 methylated sequence motifs were id ... | 2013 | 24302578 |
| the complex methylome of the human gastric pathogen helicobacter pylori. | the genome of helicobacter pylori is remarkable for its large number of restriction-modification (r-m) systems, and strain-specific diversity in r-m systems has been suggested to limit natural transformation, the major driving force of genetic diversification in h. pylori. we have determined the comprehensive methylomes of two h. pylori strains at single base resolution, using single molecule real-time (smrt®) sequencing. for strains 26695 and j99-r3, 17 and 22 methylated sequence motifs were id ... | 2013 | 24302578 |
| enteric pathogens and reactive arthritis: a systematic review of campylobacter, salmonella and shigella-associated reactive arthritis. | reactive arthritis (rea) is a spondyloarthropathic disorder characterized by inflammation of the joints and tissues occurring after gastrointestinal or genitourinary infections. diagnostic criteria for rea do not exist and, therefore, it is subject to clinical opinion resulting in cases with a wide range of symptoms and definitions. using standardized diagnostic criteria, we conducted a systematic literature review to establish the global incidence of rea for each of the three most commonly-asso ... | 2013 | 24288942 |
| molecular analysis of an alternative n-glycosylation machinery by functional transfer from actinobacillus pleuropneumoniae to escherichia coli. | n-linked protein glycosylation is a frequent post-translational modification that can be found in all three domains of life. in a canonical, highly conserved pathway, an oligosaccharide is transferred by a membrane-bound oligosaccharyltransferase from a lipid donor to asparagines in the sequon nx(s/t) of secreted polypeptides. the δ-proteobacterium actinobacillus pleuropneumoniae encodes an unusual pathway for n-linked protein glycosylation. this pathway takes place in the cytoplasm and is media ... | 2013 | 24275653 |
| molecular analysis of an alternative n-glycosylation machinery by functional transfer from actinobacillus pleuropneumoniae to escherichia coli. | n-linked protein glycosylation is a frequent post-translational modification that can be found in all three domains of life. in a canonical, highly conserved pathway, an oligosaccharide is transferred by a membrane-bound oligosaccharyltransferase from a lipid donor to asparagines in the sequon nx(s/t) of secreted polypeptides. the δ-proteobacterium actinobacillus pleuropneumoniae encodes an unusual pathway for n-linked protein glycosylation. this pathway takes place in the cytoplasm and is media ... | 2013 | 24275653 |
| a catalytically essential motif in external loop 5 of the bacterial oligosaccharyltransferase pglb. | asparagine-linked glycosylation is a post-translational protein modification that is conserved in all domains of life. the initial transfer of a lipid-linked oligosaccharide (llo) onto acceptor asparagines is catalyzed by the integral membrane protein oligosaccharyltransferase (ost). the previously reported structure of a single-subunit ost enzyme, the campylobacter lari protein pglb, revealed a partially disordered external loop (el5), whose role in catalysis was unclear. we identified a new an ... | 2013 | 24275651 |
| a catalytically essential motif in external loop 5 of the bacterial oligosaccharyltransferase pglb. | asparagine-linked glycosylation is a post-translational protein modification that is conserved in all domains of life. the initial transfer of a lipid-linked oligosaccharide (llo) onto acceptor asparagines is catalyzed by the integral membrane protein oligosaccharyltransferase (ost). the previously reported structure of a single-subunit ost enzyme, the campylobacter lari protein pglb, revealed a partially disordered external loop (el5), whose role in catalysis was unclear. we identified a new an ... | 2013 | 24275651 |
| structure-function relationships of membrane-associated gt-b glycosyltransferases. | membrane-associated gt-b glycosyltransferases (gts) comprise a large family of enzymes that catalyze the transfer of a sugar moiety from nucleotide-sugar donors to a wide range of membrane-associated acceptor substrates, mostly in the form of lipids and proteins. as a consequence, they generate a significant and diverse amount of glycoconjugates in biological membranes, which are particularly important in cell-cell, cell-matrix and host-pathogen recognition events. membrane-associated gt-b enzym ... | 2013 | 24253765 |
| structure-function relationships of membrane-associated gt-b glycosyltransferases. | membrane-associated gt-b glycosyltransferases (gts) comprise a large family of enzymes that catalyze the transfer of a sugar moiety from nucleotide-sugar donors to a wide range of membrane-associated acceptor substrates, mostly in the form of lipids and proteins. as a consequence, they generate a significant and diverse amount of glycoconjugates in biological membranes, which are particularly important in cell-cell, cell-matrix and host-pathogen recognition events. membrane-associated gt-b enzym ... | 2013 | 24253765 |
| the role of environmental reservoirs in human campylobacteriosis. | campylobacteriosis is infection caused by the bacteria campylobacter spp. and is considered a major public health concern. campylobacter spp. have been identified as one of the most common causative agents of bacterial gastroenteritis. they are typically considered a foodborne pathogen and have been shown to colonise the intestinal mucosa of all food-producing animals. much emphasis has been placed on controlling the foodborne pathway of exposure, particularly within the poultry industry, howeve ... | 2013 | 24217177 |
| comparison of characteristics of patients infected by campylobacter jejuni, campylobacter coli, and campylobacter fetus. | a large database of campylobacter isolates precisely identified at the species level was used to compare patients' characteristics. in a multivariate analysis, campylobacter coli was found more often in older patients and in patients having traveled abroad and less often in summertime than campylobacter jejuni. campylobacter fetus infection occurred in much older patients and in hospitalized patients with a systemic disease. | 2014 | 24197884 |