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detection and sequence analysis of grapevine virus b isolates from china.the presence of grapevine virus b (gvb) was detected in 188 grapevine samples from china by double antibody sandwich elisa (das-elisa) and reverse transcription-pcr (rt-pcr). the accuracy of detection by rt-pcr was confirmed by sequencing amplified pcr fragments. seventeen samples were gvb-positive by das-elisa and five by rt-pcr. the isolate qmw proved to be positive by rt-pcr only, and four isolates (dgwh, dgw, qm, and jfl) could be detected by both methods. among the five gvb-positive samples ...201424957724
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