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incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in thailand.overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in thailand, were screened by rt-pcr using primers polero-cp-f and polero-cp-r. a total of 160 samples (~66%) were infected by poleroviruses. analysis of a 1.4 kb region covering the 3' rna-dependent rna polymerase (rdrp) gene, the intergenic non-coding region (incr), and the coat protein (cp), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (cabyv), luffa aphid- ...201728352973
ring structure amino acids affect the suppressor activity of melon aphid-borne yellows virus p0 protein.melon aphid-borne yellows virus (mabyv) is a newly identified polerovirus occurring in china. here, we demonstrate that the mabyv encoded p0 (p0(ma)) protein is a strong suppressor of post-transcriptional gene silencing (ptgs) with activity comparable to tobacco etch virus (tev) hc-pro. in addition we have shown that the lp f-box motif present at the n-terminus of p0(ma) is required for suppressor activity. detailed mutational analyses on p0(ma) revealed that changing the conserved trp 212 with ...201020667575
distribution and molecular diversity of three cucurbit-infecting poleroviruses in china.cucurbit aphid-borne yellows virus (cabyv) and melon aphid-borne yellows virus (mabyv) have been found to be associated with cucurbit yellowing disease in china. our report identifies for the first time a third distinct polerovirus, tentatively named suakwa aphid-borne yellows virus (sabyv), infecting suakwa vegetable sponge. to better understand the distribution and molecular diversity of these three poleroviruses infecting cucurbits, a total of 214 cucurbitaceous crop samples were collected fr ...200919664664
complete sequence analysis reveals two distinct poleroviruses infecting cucurbits in china.the complete rna genomes of a chinese isolate of cucurbit aphid-borne yellows virus (cabyv-chn) and a new polerovirus tentatively referred to as melon aphid-borne yellows virus (mabyv) were determined. the entire genome of cabyv-chn shared 89.0% nucleotide sequence identity with the french cabyv isolate. in contrast, nucleotide sequence identities between mabyv and cabyv and other poleroviruses were in the range of 50.7-74.2%, with amino acid sequence identities ranging from 24.8 to 82.9% for in ...200818414972
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