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tannery mixed liquors from an ecotoxicological and mycological point of view: risks vs potential biodegradation application.fungi are known to be present in the activated sludge of wastewater treatment plants (wwtp). their study should be at the base of an overall vision of the plant effectiveness and of effluents sanitary impact. moreover, it could be fundamental for the implementation of successful bioaugmentation strategies aimed at the removal of recalcitrant or toxic compounds. this is one of the first studies on the cultivable autochthonous mycoflora present in the mixed liquors of two wwtp treating either vege ...201829426208
omics-based insights into flavor development and microbial succession within surface-ripened cheese.in this study, a young cheddar curd was used to produce two types of surface-ripened cheese, using two commercial smear-culture mixes of yeasts and bacteria. whole-metagenome shotgun sequencing was used to screen the microbial population within the smear-culture mixes and on the cheese surface, with comparisons of microorganisms at both the species and the strain level. the use of two smear mixes resulted in the development of distinct microbiotas on the surfaces of the two test cheeses. in one ...201829404426
differential microbial diversity in drosophila melanogaster: are fruit flies potential vectors of opportunistic pathogens?drosophila melanogaster has become a model system to study interactions between innate immunity and microbial pathogens, yet many aspects regarding its microbial community and interactions with pathogens remain unclear. in this study wild d. melanogaster were collected from tropical fruits in puerto rico to test how the microbiota is distributed and to compare the culturable diversity of fungi and bacteria. additionally, we investigated whether flies are potential vectors of human and plant path ...201729234354
the yeastgeotrichum candidumencodes functional lytic polysaccharide monooxygenases.lytic polysaccharide monooxygenases (lpmos) are a class of powerful oxidative enzymes that have revolutionized our understanding of lignocellulose degradation. fungal lpmos of the aa9 family target cellulose and hemicelluloses. aa9 lpmo-coding genes have been identified across a wide range of fungal saprotrophs (ascomycotina, basidiomycotina, etc.), but so far they have not been found in more basal lineages. recent genome analysis of the yeastgeotrichum candidum(saccharomycotina) revealed the pr ...201728919928
characterization and synergistic effect of antifungal volatile organic compounds emitted by the geotrichum candidum pf005, an endophytic fungus from the eggplant.plant-associated endophytes are recognized as sources of novel bioactive molecules having diverse applications. in this study, an endophytic yeast-like fungal strain was isolated from the fruit of eggplant (solanum melongena) and identified as geotrichum candidum through phenotypic and genotypic characterizations. this endophytic g. candidum isolate pf005 was found to emit fruity scented volatiles. the compositional profiling of volatile organic compounds (vocs) revealed the presence of 3-methyl ...201728894891
geotrichum candidumin infective endocarditis. 201728878529
fungal diversity of "tomme d'orchies" cheese during the ripening process as revealed by a metagenomic study.tomme d'orchies is an artisanal pressed and uncooked cheese produced and marketed in the north of france. this study aimed at showing the fungal microbiota evolution of this cheese using a metagenetic based illumina technology targeting the its2 domain of 5.8s fungal rdnas. to this end, samples were taken from the rind and the core of different cheeses, after 0, 1, 3, 7, 14 and 21days of ripening. the data underpinned the prevalence of yarrowia lipolytica and galactomyces geotrichum for both mic ...201728806689
treatment of tannery effluent by passive uptake-parametric studies and kinetic modeling.galactomyces geotrichum was utilized as a potential biosorbent for the treatment of tannery effluent under controlled environmental conditions. tannery effluent treatment was studied through parametric experiments to study the effect of effluent ph (3.0-10.0), initial cod (1100-4400 mg/l), and biosorbent dosage (0.3-3.0 g/l).the zeta potential of the biosorbent was determined and found to influence the optimal ph. increase in effluent cod values resulted in decreased cod removal percentages whic ...201728597388
biodiversity in targeted metabolomics analysis of filamentous fungal pathogens by (1)h nmr-based studies.the taxonomical classification among fungi kingdom in the last decades was evolved. in this work the targeted metabolomics study based on (1)h nmr spectroscopy combined with chemometrics tools was reported to be useful for differentiation of three model of fungal strains, which represent various genus of ascomycota (aspergillus pallidofulvus, fusarium oxysporum, geotrichum candidum) were selected in order to perform metabolomics studies. each tested species, revealed specific metabolic profile o ...201728585165
enhanced expression of lipase i from galactomyces geotrichum by codon optimisation in pichia pastoris.relatively poor heterologous protein yields have limited the commerical applications of galactomyces geotrichum lipase i (ggl i) efficacy trials. to address this, we have redesigned the ggl i gene to preferentially match codon frequencies of pichia pastoris (p. pastoris) while retaining the same amino acid sequence. the wild type and codon optimised ggl i (ggl i-wt and ggl i-op) were synthesised and cloned into ppiczαa with an n-terminal 6 × his tag sequence and expressed in p. pastoris x 33. th ...201728583876
diversity, in-vitro virulence traits and antifungal susceptibility pattern of gastrointestinal yeast flora of healthy poultry, gallus gallus domesticus.poultry farming and consumption of poultry (gallus gallus domesticus) meat and eggs are common gastronomical practices worldwide. till now, a detailed understanding about the gut colonisation of gallus gallus domesticus by yeasts and their virulence properties and drug resistance patterns in available literature remain sparse. this study was undertaken to explore this prevalent issue.201728506251
galactomyces geotrichum - moulds from dairy products with high biotechnological potential.the article reviews the properties of the galactomyces geotrichum species, the mould that is most important for the dairy industry. g. geotrichum mould has been isolated from milk, cheeses and alcoholic beverage. its presence in food products makes it possible to obtain a characteristic aroma and taste, which corresponds to the needs and preferences of consumers. g. geotrichum plays an important role in ecology, where the mould is employed for the degradation of various hazardous substances and ...201728362468
fungal root microbiome from healthy and brittle leaf diseased date palm trees (phoenix dactylifera l.) reveals a hidden untapped arsenal of antibacterial and broad spectrum antifungal secondary metabolites.in this study, we aimed to explore and compare the composition, metabolic diversity and antimicrobial potential of endophytic fungi colonizing internal tissues of healthy and brittle leaf diseased (bld) date palm trees (phoenix dactylifera l.) widely cultivated in arid zones of tunisia. a total of 52 endophytic fungi were isolated from healthy and bld roots of date palm trees, identified based on internal transcribed spacer-rdna sequence analysis and shown to represent 13 species belonging to fi ...201728293229
effectiveness of neutral electrolyzed water on incidence of fungal rot on tomato fruits ( solanum lycopersicum l.).we assessed the effect of neutral electrolyzed water (new) on the incidence of rot on tomato ( solanum lycopersicum l.) fruits inoculated with fusarium oxysporum , galactomyces geotrichum , and alternaria sp. at sites with lesions. the inoculated fruits were treated with new at 10, 30, and 60 mg liter(-1) active chlorine, with copper oxychloride fungicide, and with sterile distilled water (control) for 3, 5, and 10 min. in the experiment with f. oxysporum , 50 to 80% of the control fruits and 50 ...201628221850
chemical characteristics, antimicrobial and cytotoxic activities of the essential oil of egyptian cinnamomum glanduliferum bark.the essential oil isolated from the bark of cinnamomum glanduliferum (wall) meissn that grown in egypt was screened for the first time. the chemical composition was analyzed by gc and gc/mass. the antimicrobial activity of the oil was assessed using agar-well diffusion method toward representatives for each of gram-positive bacteria, gram-negative bacteria, and fungi. the cytotoxic activity was checked using three human cancer cell lines. twenty seven compounds were identified, representing 99.0 ...201728190283
cutaneous chromomycosis with pulmonary geotrichosis.an 80 year old farmer had cutaneous chromomycosis on the lateral aspect of right foot. he had associated pulmonary pathology caused by another fungus geotrichum candidum simulating miliary tuberculosis. complete regression of the pulmonary lesions and partial regression of the cutaneous lesions was observed with iodide therapy.201728128150
additive effects due to biochar and endophyte application enable soybean to enhance nutrient uptake and modulate nutritional parameters.we studied the effects of hardwood-derived biochar (bc) and the phytohormone-producing endophyte galactomyces geotrichum wll1 in soybean (glycine max (l.) merr.) with respect to basic, macro- and micronutrient uptakes and assimilations, and their subsequent effects on the regulation of functional amino acids, isoflavones, fatty acid composition, total sugar contents, total phenolic contents, and 1,1-diphenyl-2-picrylhydrazyl (dpph)-scavenging activity. the assimilation of basic nutrients such as ...201728124840
purification and characterization of a novel glutamate dehydrogenase from geotrichum candidum with higher alcohol and amino acid activity.crude enzyme from geotrichum candidum s12 exhibited high activity towards hexanol at ph 4.0, distinguishing it from currently known enzymes. to identify the dominant enzyme contributing to this activity, the crude enzyme extract was separated into different fractions by ammonium sulfate precipitation, monoq anion-exchange chromatography, and sephacryl s-200 gel filtration chromatography. afraction with high activity towards hexanol at ph 4.0 was obtained, exhibiting 38-fold improved purity and a ...201728050850
specific populations of the yeast geotrichum candidum revealed by molecular typing.geotrichum candidum is a ubiquitous yeast and an essential component in the production of many soft cheeses. we developed a multilocus sequence typing (mlst) scheme with five retained loci (nup116, ura1, ura3, sapt4 and plb3) which were sufficiently divergent to distinguish 40 sequence types (sts) among the 67 g. candidum strains tested. phylogenetic analyses defined five main clades; one clade was restricted to environmental isolates, three other clades included distinct environmental isolates ...201727862252
biological characteristics and antimicrobial activity of endophytic streptomyces sp. tqr12-4 isolated from elite citrus nobilis cultivar ham yen of vietnam.ham yen orange (citrus nobilis lour) is the highly valuable commercial fruit of vietnam. with the blooming of fruit production and farming area, this specialty crop is facing threats from several serious diseases; therefore the search for new effective biocontrollers is required to prevent the existing excessive use of fertilizers and plant protection chemicals. endophytic actinomycetes are of great scientific interest due to their high potential of application in agriculture and pharmaceutical ...201627795709
gut yeast communities in larus michahellis from various breeding colonies.yellow-legged gulls have been reported to carry antibiotic-resistant enterobacteriaceae; however, the gut mycobiota of these birds has not yet been described. in this study, we analyzed the gut yeast communities in five yellow-legged gull breeding colonies along the mediterranean littoral in southern france. gull fecal samples were inoculated onto four types of culture media, including one supplemented with itraconazole. yeast species richness, abundance, and diversity were estimated, and factor ...201627703020
crop management as a driving force of plant growth promoting rhizobacteria physiology.crop management systems influence plant productivity and nutrient use efficiency, as well as plant growth-promoting rhizobacteria (pgpr), which are known to influence the growth of plants via phytohormone production, phosphate solubilization, nitrogen (n) fixation and antimicrobial activity. the objective of this study was to compare the influence of two crop management system on microbial pgpr features. pgpr isolated from the rhizospheres of carica papaya l. grown under two distinct management ...201627652147
microbial diversity and biochemical analysis of suanzhou: a traditional chinese fermented cereal gruel.suanzhou as a traditional chinese gruel is fermented from proso millet and millet. the biochemical analysis showed suanzhou had relatively high concentrations of lactic acid, acetic acid, and free amino acids. the metagenomics of suanzhou were studied, with the analysis of the v4 region of 16s rrna gene, the genera lactobacillus and acetobacter were found dominant with the average abundance of 58.2 and 24.4%, respectively; and with the analysis of the its1 region between 18s and 5.8s rrna genes, ...201627610102
high-throughput screening of a large collection of non-conventional yeasts reveals their potential for aroma formation in food fermentation.saccharomyces yeast species are currently the most important yeasts involved in industrial-scale food fermentations. however, there are hundreds of other yeast species poorly studied that are highly promising for flavour development, some of which have also been identified in traditional food fermentations. this work explores natural yeast biodiversity in terms of aroma formation, with a particular focus on aromas relevant for industrial fermentations such as wine and beer. several non-saccharom ...201627554157
fatty acid preference of mycelium-bound lipase from a locally isolated strain of geotrichum candidum.mycelium-bound lipase (mbl) was prepared using a strain of geotrichum candidum isolated from local soil. at the time of maximum lipase activity (54 h), the mycelia to which the lipase was bound were harvested by filtration and centrifugation. dry mbl was prepared by lyophilizing the mycelia obtained. the yield of mbl was 3.66 g/l with a protein content of 44.11 mg/g. the lipase activity and specific lipase activity were 22.59 and 510 u/g protein, respectively. the moisture content of the mbl was ...200727517833
the evolution of water extractable organic matter and its association with microbial community dynamics during municipal solid waste composting.the humification of water extractable organic matter (weom) by microorganisms is widely used for assessing compost maturity and quality. however, the effect of bacterial and fungal community dynamics on humification of weom was not yet explored fully. here, we used canonical correspondence analysis (cca) and redundancy analysis (rda) to investigate the link between bacterial and fungal community dynamics and humification process of weom, respectively. results showed that water-soluble carbon (ws ...201627425858
ellagitannins from raspberry (rubus idaeus l.) fruit as natural inhibitors of geotrichum candidum.the paper presents the chemical characteristics of ellagitannins isolated from raspberry (rubus idaeus l.) fruit and their in vitro and in situ antifungal activity against geotrichum candidum łock 0511. the study investigated a complex preparation containing various raspberry ellagitannins at a concentration of 86% w/w, as well as pure lambertianin c and sanguiin h-6. the ellagitannin preparation was obtained by extracting raspberry press cake and purifying the extract using amberlite xad resin, ...201627420041
renal fungal bezoar owing to geotrichum candidum.geotrichum candidum is yeast like fungi that cause infections in immunocompromised patients. we report a case of renal fungal ball with geotrichum candidum in a 27 yr. old women post-partum. this case to our knowledge is the first case of renal fungal bezoar due to geotrichum candidum reported in india.201227330935
antifungal activity of lactobacillus sp. bacteria in the presence of xylitol and galactosyl-xylitol.lactic acid fermentation is a natural method of antimicrobial food protection. antagonistic activity of lactobacillus sp. bacteria, taking part in this process, is directed mainly against the same or other microorganisms. in this work we determine the impact of the presence of xylitol and galactosyl-xylitol on the antagonistic activity of 60 lactobacillus sp. strains against indicator molds (alternaria alternata, alternaria brassicicola, aspergillus niger, fusarium latenicum, geotrichum candidum ...201627294124
oral yeast colonization in peritoneal dialysis and hemodialysis patients and renal transplant recipients.we aimed to investigate the frequency of oral yeast colonization (oyc) and the risk factors for patients who received continuous ambulatory peritoneal dialysis (capd) or hemodialysis (hd) or were renal transplant recipients (rtrs). the patients admitted to the nephrology clinic at ataturk university medical school from january through april 2013 were included in the study. a questionnaire about risk factors was filled out, and swab cultures were taken from the tongue surface of each participant. ...201627260810
synthesis and antifungal activity of some 4,6-dimethylisoxazolo[3,4- b]pyridin-3(1h)-one derivatives.cutaneous and invasive fungal infections are constant threats to human health that substantiate the need for the development of new efficacious and safe antimycotics.201627184263
investigation of the activity of the microorganisms in a reblochon-style cheese by metatranscriptomic analysis.the microbial communities in cheeses are composed of varying bacteria, yeasts, and molds, which contribute to the development of their typical sensory properties. in situ studies are needed to better understand their growth and activity during cheese ripening. our objective was to investigate the activity of the microorganisms used for manufacturing a surface-ripened cheese by means of metatranscriptomic analysis. the cheeses were produced using two lactic acid bacteria (streptococcus thermophil ...201627148224
a plausible role for pollen-residing molds in agricultural purposes.pollen microbial content of 15 samples was investigated. pollen was collected by honeybees. total aerobic mesophilic count ranged from 3.00 to 5.48 log cfu/g. total mold and yeast count ranged from 2.3 to 6.99 log cfu/g. selected strains of isolated molds from pollen samples were characterized by conventional methods. potent phytopathogenic and food spoilage species such as penicillium sp., alternaria alternata, alternaria sp., cladosporium werneckii, mucor hiemalis, rhizomucor pusillus, aspergi ...201527141752
optimization of the production and characterization of lipase from candida rugosa and geotrichum candidum in soybean molasses by submerged fermentation.this present work describes the production and biochemical characterization of lipase by candida rugosa and geotrichum candidum in a culture supplemented with soybean molasses. after optimizing the fermentation times for both microorganisms, the effects of changing the soybean molasses concentration, the fermentative medium ph and the fermentation temperature were evaluated using the central composite planning. when soybean molasses was used at a concentration of 200 g/l at 27 ± 1 °c and ph 3.5, ...201627057641
characterisation of the yeast and mould biota in traditional white pickled cheeses by culture-dependent and independent molecular techniques.artisanal white pickled cheese of western serbia is a product of complex microbial community which detection by culture-dependent method only is hampered by its limitations. thus, in the present study, we used a culture-independent, semi-quantitative technique based on construction of an internal transcribed spacer (its)-clone library from metagenomic dna. this approach, based on direct dna extraction followed by amplification of fungal internal transcribed regions (its) cloned into plasmid and ...201627027646
nutrient assessment of olive leaf residues processed by solid-state fermentation as an innovative feedstuff additive.olive leaf residue feedstuff additives were prepared by solid-state fermentation (ssf), and its feeding effects on broiler chickens were examined.201626991541
changes in volatile profile of soybean residue (okara) upon solid-state fermentation by yeasts.soybean residue (okara), a by-product of soymilk, is produced in large volumes by the soy food industry and is often discarded due to its undesirable flavour. as it contains a considerable amount of protein and fats, biotransformation of okara to improve its flavour presents an opportunity for alternative utilisation. this paper evaluated ten yeasts in the solid-state fermentation of okara based on their volatile profiles as analysed with hs-spme gc-ms/fid. four "dairy yeasts" (geotrichum candid ...201626940283
mold-ripened soft cheeses fortified with date palm fruit product as functional dairy products.date fruit based products are gaining popularity among the consumers in almost all date growing countries due to its added nutritional value. therefore, novel products were developed by combining two types of foods i.e., soft ripened cheeses and date fruit syrups or date powder. this study is the first to report the surface mold-ripened cheese production with date syrup and date powder. model cheeses were prepared from pasteurized milk inoculated with streptococcus thermophilus, penicillium came ...201626930796
identification and partial characterization of antilisterial compounds produced by dairy yeasts.food-grade yeasts make significant contributions to flavor development in fermented foods. some yeast species also inhibit undesirable bacteria, yeasts and molds, apparently by producing antimicrobial compounds called mycocins. the aim of this study was to evaluate the ability of wild yeasts, isolated from raw milk and cheese in the quebec province area, to produce antilisterial compounds. based on an agar-membrane screening test, 22 of 95 isolates, namely one candida catenulata, one candida par ...201326782600
post operative fungal endopthalmitis due to geotrichum candidum.geotrichum species have been rarely reported as the cause of sepsis, disseminated infection in immunosuppressed patients. the patient we describe developed indolent endophthalmitis four months after her routine right eye cataract surgery. the intraoperative sample from right vitreous fluid grew geotrichum candidum. the patient underwent vitrectomy, artificial lens explantation and intravitreal injection of amphotericin b followed by oral voriconazole. despite these interventions, she underwent e ...201526779419
the effect of nacl reduction in the microbiological quality of cracked green table olives of the maçanilha algarvia cultivar.the present work aimed at studying the effect of the partial replacement of nacl with kcl and cacl2 of the fermenting brines on the microbiological quality of natural cracked green maçanilha algarvia table olives. olives were fermented in different salt combinations (brine 1-8% nacl, brine 2-4% nacl 4% kcl, brine 3-4% nacl 4% cacl2, brine 4-4% kcl 4% cacl2, and brine 5-2.7% nacl 2.7% kcl 2.7% cacl2) and the abundance of yeasts and enterobacteria was determined. at the end of fermentation, the ma ...201626613162
survival of cheese-ripening microorganisms in a dynamic simulator of the gastrointestinal tract.a mixture of nine microorganisms (six bacteria and three yeasts) from the microflora of surface-ripened cheeses were subjected to in vitro digestive stress in a three-compartment "dynamic gastrointestinal digester" (didgi). we studied the microorganisms (i) grown separately in culture medium only (ii) grown separately in culture medium and then mixed, (iii) grown separately in culture medium and then included in a rennet gel and (iv) grown together in smear-ripened cheese. the yeasts geotrichum ...201626611167
[state of fungal lipases of rhizopus microsporus, penicillium sp. and oospora lactis in border layers water-solid phase and factors affecting catalytic properties of enzymes].we demonstrated that a change in the catalytic activity of fungal lipases synthesized by rhizopus microsporus, penicillium sp. and oospora lactis and their ability to absorb on different sorbents depended on the nature of groups on the solid phase surface in the model systems water: lipid and water: solid phase. thus, the stability of penicillium sp. lipases increased 85% in the presence ofsorsilen or deae-cellulose, and 55% of their initial activity respectively was preserved. in the presence o ...201526596088
diversity and antifungal resistance patterns of prevalent opportunistic pathogenic yeasts colonizing the oral cavities of asymptomatic human immunodeficiency virus-infected individuals, and their relation to cd4(+) counts.yeasts are important opportunistic pathogens, in individuals infected with human immunodeficiency virus (hiv). yeast species inhabiting the oral mucosa of hiv-infected persons can act as source of oral lesions, especially as the individual progresses towards immunocompromised state. present study was conducted to evaluate the diversity of yeasts in oral cavities of asymptomatic hiv-infected persons and their association with cd4(+) cell counts.201726392655
volatile constituents of dietes bicolor (iridaceae) and their antimicrobial activity.volatile oils from the leaves, flowers and rhizomes of dietes bicolor (steud.) sweet ex klatt (iridaceae) were analyzed using glc/fid and glc/ms. a total of 84 compounds were identified accounting for 94.65, 95.63 and 87.09% in the hydrodistilled oils from flowers, leaves and rhizomes, respectively. spathulenol (48.44%) represented the major component in the leaf oil, followed by dihydro-edulan i (6.25%), cubenol (6.00%) and τ-cadinol (5.90%). for the flower and rhizome oils, fatty acids, their ...201526368045
sequencing, biochemical characterization, crystal structure and molecular dynamics of cellobiohydrolase cel7a from geotrichum candidum 3c.the ascomycete geotrichum candidum is a versatile and efficient decay fungus that is involved, for example, in biodeterioration of compact discs; notably, the 3c strain was previously shown to degrade filter paper and cotton more efficiently than several industrial enzyme preparations. glycoside hydrolase (gh) family 7 cellobiohydrolases (cbhs) are the primary constituents of industrial cellulase cocktails employed in biomass conversion, and feature tunnel-enclosed active sites that enable proce ...201526367132
yeasts associated with an abandoned mining area in pernek and their tolerance to different chemical elements.four plants, cirsium arvense (creeping thistle), equisetum arvense (field horsetail), oxalis acetosella (wood sorrel) and phragmites australis (common reed), which grew in an abandoned sb-mining area in pernek (malé karpaty mts., slovakia), were investigated for the yeast species. yeasts were isolated from both the leaves of the plants and the soil adjacent to the plants. in total, 65 yeast cultures, belonging to 11 ascomycetous and 5 basidiomycetous yeast species, were isolated. the species mos ...201626358066
the good, the bad, and the ugly: tales of mold-ripened cheese.the history of cheese manufacture is a "natural history" in which animals, microorganisms, and the environment interact to yield human food. part of the fascination with cheese, both scientifically and culturally, stems from its ability to assume amazingly diverse flavors as a result of seemingly small details in preparation. in this review, we trace the roots of cheesemaking and its development by a variety of human cultures over centuries. traditional cheesemakers observed empirically that cer ...201326184810
differential gene retention as an evolutionary mechanism to generate biodiversity and adaptation in yeasts.the evolutionary history of the characters underlying the adaptation of microorganisms to food and biotechnological uses is poorly understood. we undertook comparative genomics to investigate evolutionary relationships of the dairy yeast geotrichum candidum within saccharomycotina. surprisingly, a remarkable proportion of genes showed discordant phylogenies, clustering with the filamentous fungus subphylum (pezizomycotina), rather than the yeast subphylum (saccharomycotina), of the ascomycota. t ...201526108467
biodiversity of the surface microbial consortia from limburger, reblochon, livarot, tilsit, and gubbeen cheeses.comprehensive collaborative studies from our laboratories reveal the extensive biodiversity of the microflora of the surfaces of smear-ripened cheeses. two thousand five hundred ninety-seven strains of bacteria and 2,446 strains of yeasts from the surface of the smear-ripened cheeses limburger, reblochon, livarot, tilsit, and gubbeen, isolated at three or four times during ripening, were identified; 55 species of bacteria and 30 species of yeast were found. the microfloras of the five cheeses sh ...201426082119
mycobiota variation in stored rice straw and its cellulolytic profile.rice straw (rs) one of most important agrowaste worldwide. variation in mycobiota inhabiting long stored rs and its cellulolytic profile were studied. the highest number of fungi (23 species) was recovered from 1st storage period (1-3 year). alternaria alternata, aspergillus sp., cladosporium herbarum, fusarium incarnatum, geotrichum candidum, penicillium sp., stemphylium lycopersici and ulocladium atrum are the most frequent genera. among 21 fungal species recovered in the 2nd period (3-5 year) ...201426031023
growth and adaptation of microorganisms on the cheese surface.microbial communities living on cheese surfaces are composed of various bacteria, yeasts and molds that interact together, thus generating the typical sensory properties of a cheese. physiological and genomic investigations have revealed important functions involved in the ability of microorganisms to establish themselves at the cheese surface. these functions include the ability to use the cheese's main energy sources, to acquire iron, to tolerate low ph at the beginning of ripening and to adap ...201525790503
crystallization and preliminary crystallographic analysis of acetophenone reductase from geotrichum candidum nbrc 4597.acetophenone reductase (aprd) from geotrichum candidium nbrc 4597 was crystallized by the hanging-drop vapour-diffusion method using peg 3350 as a precipitant. the crystal belonged to space group p6522, with unit-cell parameters a = b = 104.5, c = 273.7 å, and diffracted to 2.6 å resolution. phasing using the single-wavelength anomalous diffraction method was successful. model building and crystallographic refinement are in progress.201525760708
persistence of nosocomial pathogens on various fabrics.fabrics can become contaminated with high numbers of microorganisms that may be pathogenic to patients in a hospital setting and can play an important role in the chain of infection. the aim of this study was to investigate the survival of several clinical bacterial and fungal isolates on several fabrics commonly used in hospitals.201225610201
survey of bovine mycotic mastitis in different mammary gland statuses in two north-eastern regions of algeria.the aim of this study was to evaluate the prevalence of mycotic mastitis in different mammary gland statuses. the study was conducted on 304 dairy cows from ten farms in two north-eastern regions in algeria; guelma and souk ahras with 922 and 199 samples, respectively, forming thus a total number of 1,121 milk samples. a total of 321 milk samples were collected from clinical mastitis, 544 milk samples from subclinical mastitis and 256 milk samples from healthy mammary glands. mycological analyse ...201525481847
investigation of geotrichum candidum gene expression during the ripening of reblochon-type cheese by reverse transcription-quantitative pcr.cheese ripening involves the activity of various bacteria, yeasts or molds, which contribute to the development of the typical color, flavor and texture of the final product. in situ measurements of gene expression are increasingly being used to improve our understanding of the microbial flora activity in cheeses. the objective of the present study was to investigate the physiology and metabolic activity of geotrichum candidum during the ripening of reblochon-type cheeses by quantifying mrna tra ...201525461609
unpasteurised commercial boza as a source of microbial diversity.boza is a cereal-based fermented beverage widely consumed in many countries of the balkans. the aim of this study was to investigate the microbiota of three bulgarian boza samples through a combination of culture-dependent and -independent methods with the long-term objective of formulating a multi-strain starter culture specifically destined for the manufacture of new cereal-based drinks. the isolation campaign for lactic acid bacteria (lab) allowed the identification of lactobacillus parabuchn ...201525437059
yeast tolerance to the ionic liquid 1-ethyl-3-methylimidazolium acetate.lignocellulosic plant biomass is the target feedstock for production of second-generation biofuels. ionic liquid (il) pretreatment can enhance deconstruction of lignocellulosic biomass into sugars that can be fermented to ethanol. although biomass is typically washed following il pretreatment, small quantities of residual il can inhibit fermentative microorganisms downstream, such as the widely used ethanologenic yeast, saccharomyces cerevisiae. the aim of this study was to identify yeasts toler ...201425348480
draft genome sequence of geotrichum candidum strain 3c.we report here the draft genome sequence of geotrichum candidum strain 3c, which is a filamentous yeast-like fungus that holds great promise for biotechnology. the genome was sequenced using ion torrent and 454 platforms. the estimated genome size was 41.4 mb, and 14,579 protein-coding genes were predicted ab initio.201425278525
decolorization of oxygen-delignified bleaching effluent and biobleaching of oxygen-delignified kraft pulp by non-white-rot fungus geotrichum candidum dec 1.decolorization of oxygen-delignified bleaching effluent (abbreviated as obe) and biobleaching of oxygen-delignified kraft pulp (okp) were conducted using a non-white rot fungus geotrichum candidum dec 1 (abbreviated as dec 1) which has ability to decolorize various synthetic dyes and molasses. dec 1 decolorized up to 77% of obe for 6 days. in addition, dec 1 increased the brightness of okp from 47.8% to 51.2% and decreased the kappa value of okp from 12.4 to 10.4 points during a 6-day incubation ...201325078823
mycelium-bound lipase from a locally isolated strain of geotrichum candidum.mycelium-bound lipase (mbl), from a locally isolated geotrichum candidum strain, was produced and characterized as a natural immobilized lipase. a time course study of its lipolytic activity in 1 l liquid broth revealed the maximum mbl activity at 4 h for mycelium cells harvested after 54 h. the yield and specific activity of mbl were 3.87 g/l dry weight and 508.33 u/g protein, respectively, while less than 0.2 u/ml lipase activity was detected in the culture supernatant. prolonged incubation ca ...201424959682
bioresolution production of (2r,3s)-ethyl-3-phenylglycidate for chemoenzymatic synthesis of the taxol c-13 side chain by galactomyces geotrichum zjutzq200, a new epoxide-hydrolase-producing strain.a newly isolated galactomyces geotrichum zjutzq200 strain containing an epoxide hydrolase was used to resolve racemic ethyl 3-phenylglycidate (rac-epg) for producing (2r,3s)-ethyl-3-phenylglycidate ((2r,3s)-epg). g. geotrichum zjutzq200 was verified to be able to afford high enantioselectivity in whole cell catalyzed synthesis of this chiral phenylglycidate synthon. after the optimization of the enzymatic production and bioresolution conditions, (2r,3s)-epg was afforded with high enantioselectiv ...201424936708
impact of microbial cultures on proteolysis and release of bioactive peptides in fermented milk.this study aimed at evaluating co-cultures of selected microorganisms for their proteolytic activity and capability to produce fermented milk enriched with ace-inhibitory (acei) peptides. selected yeasts (torulaspora delbruekii kl66a, galactomyces geotrichum kl20b, pichia kudriavzevii kl84a and kluyveromyces marxianus kl26a) and lactic acid bacteria strains (lactobacillus plantarum lat03, lb. plantarum klat01 and the not virulent enterococcus faecalis ke06) were screened as single cultures for t ...201424929726
production of folate in oat bran fermentation by yeasts isolated from barley and diverse foods.the focus of the research was to identify yeasts from barley kernels in order to study their folate production capability while maintaining high viscosity caused by soluble fibres in oat bran fermentation.201424916316
studies on drug metabolism by fungi colonizing decomposing human cadavers. part ii: biotransformation of five model drugs by fungi isolated from post-mortem material.the present study investigated the in vitro metabolic capacity of 28 fungal strains isolated from post-mortem material towards five model drugs: amitriptyline, metoprolol, mirtazapine, promethazine, and zolpidem. each fungal strain was incubated at 25 °c for up to 120 h with each of the five models drugs. cunninghamella elegans was used as positive control. aliquots of the incubation mixture were centrifuged and 50 μl of the supernatants were diluted and directly analyzed by liquid chromatograph ...201524898183
phanerochaete chrysosporium inoculation shapes the indigenous fungal communities during agricultural waste composting.inoculation with exogenous white-rot fungi has been proven to be an efficient method to promote lignocellulose biodegradation during agricultural waste composting. indigenous fungal communities, the most important organisms responsible for mineralization and decomposition of lignocellulosic materials in composts, can be affected by sample properties and other biotic factors. this research was conducted to determine the effects of the phanerochaete chrysosporium inoculation on the indigenous fung ...201424728506
misidentification of saprochaete clavata as magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases.saprochaete clavata and magnusiomyces capitatus are human pathogens that are frequently mistaken for each other due to their similar phenotypes and erroneous or limited databases. based on internal transcribed spacer (its) sequences, we propose species-specific carbon assimilation patterns and matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) fingerprints that enable the identification of s. clavata, m. capitatus, and galactomyces candidus to the species ...201424696028
metatranscriptome analysis of fungal strains penicillium camemberti and geotrichum candidum reveal cheese matrix breakdown and potential development of sensory properties of ripened camembert-type cheese.camembert-type cheese ripening is driven mainly by fungal microflora including geotrichum candidum and penicillium camemberti. these species are major contributors to the texture and flavour of typical bloomy rind cheeses. biochemical studies showed that g. candidum reduces bitterness, enhances sulphur flavors through amino acid catabolism and has an impact on rind texture, firmness and thickness, while p. camemberti is responsible for the white and bloomy aspect of the rind, and produces enzyme ...201424670012
decolorization and degradation of xenobiotic azo dye reactive yellow-84a and textile effluent by galactomyces geotrichum.galactomyces geotrichum mtcc 1360 exhibited 86% decolorization of azo dye reactive yellow-84a (50mgl(-1)) within 30h at 30°c and ph 7.0 under static condition. examination of azoreductase, laccase and tyrosinase enzyme activities confirmed their prominent role in reactive yellow-84a degradation. considerable reduction of cod (73%) and toc (62%) during degradation of the dye was indicative of conversion of complex dye into simple products, which were further analyzed by hplc, ftir, gc-ms and hptl ...201424630455
bioflocculation behaviours of microbial communities in water treatment.we studied the flocculation behaviours of microbial communities in 21 soil, wastewater and activated sludge samples to clarify the effects of culture medium types on flocculation ability and screening efficiency, and to analyze diverse functions and microbial compositions. the bioflocculants produced by 33% of the microbial communities had flocculating efficiencies higher than 90%. six out of the eight microbial communities with efficiencies over 94% were screened from the culture medium using d ...201424569266
biodegradation by bioaugmentation of dairy wastewater by fungal consortium on a bioreactor lab-scale and on a pilot-scale.a fungal consortium including aspergillus niger, mucor hiemalis and galactomyces geotrichum was tested for the treatment of dairy wastewater. the bio-augmentation method was tested at lab-scale (4 l), at pilot scale (110 l) and at an industrial scale in wastewater treatment plants (wwtp). the positive impact of fungal addition was confirmed when fungi was beforehand accelerated by pre-culture on whey (5 g/l lactose) or on the dairy effluent. indeed, chemical oxygen demand (cod) removal yields in ...201324520735
growth of three fungi on poultry fat or beef tallow.aspergillus niger, geotrichum candidum and mucor miehei, when grown on a medium containing beef tallow or poultry fat for 5 days at 28°c, in roux bottles, shake-flasks or fermenters, synthesized from 0.4 to 3.2 g lipids/i. the degree of fat utilization varied from 15 to 70%. the type of fat in the medium, the species of fungi and its cultivation method all influenced the fatty acid composition of fats remaining in the medium, as well as the intracellular lipids of the fungi.199324420293
microbiology of pozol, a mexican fermented maize dough.mexican fermented maize dough, pozol, including traditional banana leaf-wrapped samples and material in plastic bags, was purchased. all samples were ph 4.7 to 5.7 approx. 12 h after preparation, ph declining to 3.6 to 3.9 after 6 to 9 days storage at ambient temperature. these latter samples had dry matter contents of 31% to 48% (w/w), 0.35% to 0.75% titratable acidity as lactic acid and lactic acid bacteria as predominant microbial flora at about 10(8) c.f.u./ml. the lactic acid bacteria inclu ...199524414915
mechanisms of increased linamarin degradation during solid-substrate fermentation of cassava.several fungi and bacteria, isolated from ugandan domestic fermented cassava, released hcn from linamarin in defined growth media. in 72 h, a bacillus sp. decreased the linamarin to 1% of initial concentrations, mucor racemosus to 7%, rhizopus oryzae and r. stolonifer to 30%, but neurospora sitophila and geotrichum candidum hardly degraded the linamarin. adding pectolytic and cellulolytic enzymes, but not linamarase, to root pieces under aseptic conditions, led to root softening and significantl ...199524414645
malting of barley with combinations of lactobacillus plantarum, aspergillus niger, trichoderma reesei, rhizopus oligosporus and geotrichum candidum to enhance malt quality.good quality malt is characterised by the presence of high levels of fermentable sugars, amino acids and vitamins. to reach the starch-rich endosperm of the kernel, β-glucan- and arabinoxylan-rich cell walls have to be degraded. β-glucanase is synthesized in vast quantities by the aleurone layer and scutellum during germination. secretion of hydrolytic enzymes is often stimulated by addition of the plant hormone gibberellic acid (ga3) during germination. we have shown an enhanced β-glucanase and ...201424412956
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
isolation of phosphate-solubilizing fungus and its application in solubilization of rock phosphates.microorganisms have been obtained to improve the agronomic value of rock phosphates (rps), but the phosphorus solubilizing rate by these approaches is very slow. it is important to explore a high-efficient phosphate-solubilizing approach with a kind of microorganisms. this study aimed to isolate a high-efficient level of phosphate-solubilizing fungus from rhizosphere soil samples phosphate mines (liuyang county, hunan province, china) and apply it in solubilization of rps. the experiments were c ...201224261118
retraction. a new antifungal labdane diterpene from the leaves of saraca indica.a new labdane diterpene, along with 10 known sterols and flavonoids, was isolated from the hydroalcoholic extract of the leaves of saraca indica. the chemical structure of the new compound was identified as 6,9-epoxy marrubiinic acid on the basis of spectroscopic analyses including two-dimensional nmr. the antimicrobial potential of the new compound was evaluated against gram-positive and gram-negative bacteria as well as fungi. it showed a significant antifungal activity against geotrichum cand ...201524237384
synthesis and characterization of some novel antimicrobial thiosemicarbazone o-carboxymethyl chitosan derivatives.three novel thiosemicarbazone o-carboxymethyl chitosan derivatives were obtained via a condensation reaction of thiosemicarbazide o-carboxymethyl chitosan with o-hydroxybenzaldehyde, p-methoxybenzaldehyde, and p- chlorobenzaldehyde respectively. their structures were characterized by elemental analysis, ftir, (13)c nmr and x-ray diffraction. the antimicrobial behaviors of the prepared derivatives against three types of bacteria staphylococcus aureus (s. aureus, rcmba 2004), bacillus subtilis (b. ...201424211430
geotrichum candidum as a possible cause of bovine abortion.geotrichum spp. are ubiquitous, saprotrophic fungi found in soil, organic matter, and silage, as a contaminant in food products and in the digestive tracts of mammals. the current study reports a case of geotrichum candidum infection with dermatitis in an aborted bovine fetus with skin and lung lesions. a 6-month-old aborted male holstein friesian fetus displayed unusual lesions on the skin of the abdomen, thorax, and head, which was excessively thickened and wrinkled. these changes corresponded ...201324153033
unusual transformation of substituted-3-formylchromones to pyrimidine analogues: synthesis and antimicrobial activities of 5-(o-hydroxyaroyl)pyrimidines.substituted-3-formylchromones (4a-e) on reaction with 1,3-bis-dimethylaminomethylene-thiourea (5) in refluxing toluene solution give novel substituted 5-(o-hydroxyaroyl)pyrimidines (6a-e) in high yields. a mechanistic rationalization of the formation of products (6a-e) is proffered. antimicrobial activities of all the synthesized compounds (6a-e) were evaluated against various fungal and bacterial strains. compound 6d display significant antifungal activity (mic 15) against geotrichum candidum i ...201324120267
use of pcr-restriction fragment length polymorphism analysis for identification of yeast species isolated from bovine intramammary infection.this study reports a rapid pcr-based technique using a one-enzyme rflp for discrimination of yeasts isolated from bovine clinical and subclinical mastitis milk samples. we analyzed a total of 1,486 milk samples collected over 1 yr in south sardinia and northern italy, and 142 yeast strains were preliminarily grouped based on their cultural morphology and physiological characteristics. assimilation tests were conducted using the identification kit api id 32c and apilab plus software (biomérieux, ...201324119798
escmid and ecmm joint clinical guidelines for the diagnosis and management of rare invasive yeast infections.the mortality associated with invasive fungal infections remains high with that involving rare yeast pathogens other than candida being no exception. this is in part due to the severe underlying conditions typically predisposing patients to these healthcare-related infections (most often severe neutropenia in patients with haematological malignancies), and in part due to the often challenging intrinsic susceptibility pattern of the pathogens that potentially leads to delayed appropriate antifung ...201424102785
screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration.gamma linolenic acid (gla, 18:3, cis- 6,9,12- octadecatrienoic acid), an important compound in n- 6 eicosanoid family biosynthesis, occurs in the lipids of a few plant and microbial sources. this study focused on the screening of microbial strains with suitable lipase activity for enrichment of gla by selective hydrolysis of the borage oil (21.6 % of gla/total fatty acids). firstly, 352 microrganisms were tested for their lipolytic capacity using screening techniques on agar plates containing bo ...200924031421
potentially pathogenic yeasts from soil of children's recreational areas in the city of łódź (poland).yeasts may become potential human and animal pathogens, particularly for individuals with a depressed immune system. their presence in the environment, especially in soil, may favour their spread into human ontocenoses.201324018998
molecular study of geotrichum strains isolated from armada cheese.the aim of this work was the genetic characterization at the strain level of 39 presumed geotrichum candidum isolates isolated throughout the artisanal manufacturing and ripening of armada cheese and tentatively identified at genus and/or species level by phenotypic characteristics. the molecular identification of the strains included among others the amplification and sequencing of the d1/d2 domains of the 26s rrna gene. a restriction fragment length polymorphism (rflp) analysis with the its1-5 ...201324010632
improvement of medium chain fatty acid content and antimicrobial activity of coconut oil via solid-state fermentation using a malaysian geotrichum candidum.coconut oil is a rich source of beneficial medium chain fatty acids (mcfas) particularly lauric acid. in this study, the oil was modified into a value-added product using direct modification of substrate through fermentation (dimosfer) method. a coconut-based and coconut-oil-added solid-state cultivation using a malaysian lipolytic geotrichum candidum was used to convert the coconut oil into mcfas-rich oil. chemical characteristics of the modified coconut oils (mcos) considering total medium cha ...201323971051
characterization of mutual relations between geotrichum candidum and lactobacillus rhamnosus gg in milk: a quantitative approach.the growth interactions between geotrichum candidum and lactobacillus rhamnosus gg were studied in milk. the effect of temperature on the growth rate of the fungus was modelled using the cardinal temperature model with inflection. the secondary modelling was applied also on the other data set containing the growth rates of g. candidum in co-culture with a commercial starter culture. the low temperature in combination with l. rhamnosus gg in co-culture showed the most negative effect on the growt ...201423733814
multicenter study evaluating the vitek ms system for identification of medically important yeasts.the optimal management of fungal infections is correlated with timely organism identification. matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) is revolutionizing the identification of yeasts isolated from clinical specimens. we present a multicenter study assessing the performance of the vitek ms system (biomérieux) in identifying medically important yeasts. a collection of 852 isolates was tested, including 20 candida species (626 isolates, includin ...201323658267
yeasts from autochthonal cheese starters: technological and functional properties.the aim of this work was to identify 20 yeasts isolated from autochthonal cheese starters and evaluate their technological and functional properties.201323600736
aromatic amino acids as precursors of antimicrobial metabolites in geotrichum candidum.geotrichum candidum atcc 204307 was previously found to generate phenyllactic acid (pla) and indoleacetic acid (ila) in complex culture media. in this study, a relationship between concentrations of pla, ila, and hydroxy pla (oh-pla) and initial concentrations of phenylalanine, tryptophan, and tyrosine, added respectively as unique sources of nitrogen in synthetic medium, was established. phenylpyruvic acid (ppa), an intermediate compound of pla metabolism, was able to induce not only pla but al ...201323590565
taxonomic and molecular characterization of lactic acid bacteria and yeasts in nunu, a ghanaian fermented milk product.produced from raw unpasteurized milk, nunu is a spontaneously fermented yoghurt-like product made in ghana and other parts of west africa. despite the importance of nunu in the diet of many africans, there is currently only limited information available on the microorganisms associated with nunu processing. with the aim of obtaining a deeper understanding of the process and as a first step towards developing starter cultures with desired technological properties for nunu production, a microbiolo ...201223541194
differential fate of metabolism of a disperse dye by microorganisms galactomyces geotrichum and brevibacillus laterosporus and their consortium gg-bl.the present work aims to evaluate brown 3 rel degrading potential of developed microbial consortium gg-bl using two microbial cultures, galactomyces geotrichum mtcc 1360 (gg) and brevibacillus laterosporus mtcc 2298 (bl). microbial consortium gg-bl showed 100% decolorization of a dye brown 3 rel, while individually g. geotrichum mtcc 1360 and b. laterosporus mtcc 2298 showed 26% and 86% decolorization under aerobic condition (shaking) respectively. measurements of biochemical oxygen demand (bod) ...201223513451
acetophenone reductase with extreme stability against a high concentration of organic compounds or an elevated temperature.the gene encoding acetophenone reductase (aprd), a useful biocatalyst for producing optically pure alcohols, was cloned from the cdna of geotrichum candidum nbrc 4597. the gene contained an open reading frame that consisted of 1,029 nucleotides corresponding to 342 amino acid residues. the subunit molecular weight was calculated to be 36.7 kda. the predicted amino acid sequence did not have significant similarity to those of the acetophenone reductase reported previously. the gene was inserted i ...201323504059
novel terephthaloyl thiourea cross-linked chitosan hydrogels as antibacterial and antifungal agents.four novel terephthaloyl thiourea chitosan (ttu-chitosan) hydrogels were synthesized via a cross-linking reaction of chitosan with different concentrations of terephthaloyl diisothiocyanate. their structures were investigated by elemental analyses, ftir, sem and x-ray diffraction. the antimicrobial activities of the hydrogels against three species of bacteria (bacillis subtilis, staphylococcus aureus and escherichia coli) and three crop-threatening pathogenic fungi (aspergillus fumigatus, geotri ...201323500435
genetic diversity of dairy geotrichum candidum strains revealed by multilocus sequence typing.the introduction of multilocus sequence typing (mlst) for strain characterization provided the first sequence-based approach for genotyping many fungi, leading to reproducible, reliable, and exchangeable data. a mlst scheme based on the analysis of six housekeeping genes was developed for genotyping geotrichum candidum. the scheme was first developed using 18 isolates for which the complete sequences of the alanyl-trna synthetase (ala1), pyruvate kinase (cdc19), acetyl-coa acetyltransferase (erg ...201323467823
the burkholderia contaminans ms14 ocfc gene encodes a xylosyltransferase for production of the antifungal occidiofungin.burkholderia contaminans strain ms14 produces the antifungal compound occidiofungin, which is responsible for significant antifungal activities against a broad range of plant and animal fungal pathogens. occidiofungin is a cyclic glycolipopeptide made up of eight amino acids and one xylose. a 56-kb ocf gene cluster was determined to be essential for occidiofungin production. in this study, the ocfc gene, which is located downstream of ocfd and upstream of the ocfb gene in the ocf gene cluster, w ...201323435879
[fungal dacryocystitis with cacosmia after penetrating keratoplasty--taxonomy and identification of pathogenic fungi based on dna sequence analysis].a 76-year-old woman with a history of penetrating keratoplasty had cacosmia associated with dacryocystitis. two species of yeast-like fungi were isolated from the contents of her lacrimal sac. each cultured fungus had a unique, distinctive odor. althogh treated with an oral antifungal agent and washing of the nasolacrimal duct, the cacosmia was not improved. the continuous dacryocystitis with cacosmia was treated by dacryocystectomy. two yeast-like fungi were again isolated from the contents of ...201223379204
control of t-2 toxin in fusarium langsethiae and geotrichum candidum co-culture.due to contamination of barley grains by fusarium langsethiae, t-2 toxin can be present in the brewing process. it has been observed that the presence of the yeast geotrichum candidum during malting can reduce the final concentration of this mycotoxin in beer. in this work, a co-culture method was carried out for both microorganisms in order to evaluate the effect on t-2 mycotoxin concentration in comparison with the pure culture of f. langsethiae in the same conditions. the microbial growth of ...201223334039
yeasts and yeast-like organisms associated with fruits and blossoms of different fruit trees.yeasts are common inhabitants of the phyllosphere, but our knowledge of their diversity in various plant organs is still limited. this study focused on the diversity of yeasts and yeast-like organisms associated with matured fruits and fully open blossoms of apple, plum, and pear trees, during 2 consecutive years at 3 localities in southwest slovakia. the occurrence of yeasts and yeast-like organisms in fruit samples was 2½ times higher and the yeast community more diverse than that in blossom s ...201223210991
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