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molecular characterization of rabies virus isolated from non-haematophagous bats in brazil.rabies is an important zoonosis that causes thousands of deaths worldwide each year. although the terrestrial cycle, mainly transmitted by dogs, is controlled in brazil, the aerial cycle remains a serious public health issue, besides the economic problem. in the aerial cycle, the haematophagous bat desmodus rotundus is the main source of infection, where several different species of non-haematophagous bats can be infected and can transmit the virus.201122231241
[evaluation of rabies post-exposure prophylaxis in humans injured by dogs and cats in the municipality of jaboticabal, sp, from 2000 through 2006].the present study aimed to evaluate rabies post-exposure prophylaxis (pep) in humans in the municipality of jaboticabal, são paulo from 2000 through 2006. a descriptive and retrospective study was conducted by collecting data available in patient records. vaccination costs were also calculated; 2,493 patients injured by animals received pep, totaling r$ 179,105.14 and 7,108 doses; 2,184 (71.5%) out of the total reports were caused by dogs and cats clinically healthy at the moment of the attack. ...201122218670
outbreaks of attacks by hematophagous bats in isolated riverine communities in the brazilian amazon: a challenge to rabies control.human rabies has re-emerged in latin america due to bat associated transmission. we present data related to attacks by hematophagous bats in three riverine communities in the rio negro basin, brazilian amazon. a cross-sectional survey was carried out to obtain demographic and epidemiological data through interviews with 201 inhabitants. a total of 721 bat attacks with bites took place from 2004 to 2006, 238 (33%) reported by residents in campinas do rio preto, 329 (46%) in águas vivas and 154 (2 ...201122171969
[Zoonosis transmission risk factors according to population habits inIlha Solteira city, Brazil].Determining conditions regarding possible zoonosis transmission risk based on Ilha Solteira-São Paulo citizens' habits aimed at establishing concrete recommendations for the corresponding local authorities to reduce some risk factors.201122031002
molecular and serological detection of leishmania spp. in captive wild animals from ilha solteira, sp, brazil.leishmaniasis is a zoonotic disease that affects 12 million people worldwide. several mammalian species can serve as a reservoir for this disease. dogs are the main reservoir for visceral leishmaniasis in urban areas, which has become a serious public health concern in brazil. the aim of this study was to evaluate the presence of leishmania spp. in captive wild animals from ilha solteira, são paulo, brazil. blood and various tissues samples were collected from animals of five different species: ...201121961752
first occurrence of an autochthonous canine case of leishmania (leishmania) infantum chagasi in the municipality of campinas, state of são paulo, brazil.an autochthonous case of visceral leishmaniasis is reported in a dog (canis familiaris) as an apparently natural infection in a non-endemic area. dna obtained from spleen and liver samples produced the expected fragment in a leishmania-specific rdna-based nested-pcr assay. the pcr product, a 490 bp fragment, was sequenced and the nucleotide sequence was identical to that of leishmania (leishmania) infantum chagasi. these results are surprising since no autochthonous human or canine cases of visc ...201121915468
Occurrence of Ancylostoma in dogs, cats and public places from Andradina city, São Paulo state, Brazil.The aim of this study was to determine the frequency and intensity of Ancylostoma spp. in 33 dogs and 52 cats by means of coproparasitological examinations and parasitological necropsy, and assess the presence of contaminated feces with eggs of that parasite in public places of Andradina Municipality, São Paulo State, Brazil. Willis-Mollay and Sedimentation methods indicated Ancylostoma spp. eggs in 87.8% (29/33) dogs and 94.2% (49/52) cats. The species A. caninum and A. braziliense were found i ...201121915459
giardiasis as zoonosis: between proof of principle and paradigm in the northwestern region of são paulo state, brazil.in order to evaluate the potential zoonotic transmission of giardia duodenalis, isolates from humans and dogs in the northwestern region of the são paulo state, brazil were characterized based on the β-giardin gene.201121861011
molecular characterization of giardia duodenalis in dogs from brazil.the intestinal protozoan parasite giardia duodenalis (syn. giardia intestinalis and giardia lamblia) is a widespread enteric pathogen in human and domestic animals. this organism is one of the most common parasites in domestic dogs in brazil. in this study, we determined the occurrence and genetic characterization of g. duodenalis isolated from dogs from south-central são paulo state, brazil. a total of 300 fecal samples were collected. fecal specimens were screened for the presence of g. duoden ...201121695567
[soil contamination by eggs of soil-transmitted helminths with zoonotic potential in the town of fernandópolis, state of são paulo, brazil, between 2007 and 2008.]introduction: the concentration of dogs and cats in urban areas, associated with an ever-increasing wandering population of these animals, has an important epidemiological role in the soil contamination of public spaces and the spread of infections of several types of parasites. this study aimed to determine the frequency of soil-transmitted helminths with zoonotic potential in public squares and municipal primary schools in fernandópolis, state of são paulo, brazil, conducted between 2007 and 2 ...201121552740
protective and risk factors for toxocariasis in children from two different social classes of brazil.the aim of this study was to analyze the prevalence of toxocara spp. antibodies in children from two different socioeconomic classes in the presidente prudente municipality, são paulo state, brazil, and the protective and risk factors associated with toxocariasis. one hundred and twenty-six middle-class (mc) and 126 disadvantaged children (dc) were included in this study. anti-toxocara elisa test was performed in order to evaluate seroprevalence. a survey was applied to the children's guardians/ ...201121537752
a survey of owner attitudes and experiences in managing diabetic dogs.a survey of the experiences of owners of diabetic dogs was carried out by distributing questionnaires to veterinary clinics in s+úo paulo, brazil. a total of 93 dog owners were surveyed over a 4month period and descriptive statistical analysis, frequency distribution and correlations were performed. in most cases the clinical condition of the dogs had improved following treatment and owners were largely satisfied with veterinary intervention. injection of neutral protamine hagedorm twice daily w ...201121396860
use of crude, fml and rk39 antigens in elisa to detect anti-leishmania spp. antibodies in felis catus.visceral leishmaniasis is a disease caused by leishmania (leishmania) chagasi and represents a serious public health problem. the dog is the main urban reservoir of the disease; however, investigations regarding the occurrence and epidemiological importance of leishmaniasis in cats have recently been initiated. this study aimed to detect cats seropositive for leishmania spp. using different antigens. additional studies were performed using sera from cats with toxoplasma gondii (n=15) to evaluate ...201021195550
occurrence and molecular characterization of cryptosporidium spp. isolated from domestic animals in a rural area surrounding atlantic dry forest fragments in teodoro sampaio municipality, state of são paulo, brazil.the aim of this study was to assess the occurrence of cryptosporidium in domestic animals in rural properties surrounding rain forest fragments within the municipality of teodoro sampaio, southeastern brazil. conventional sucrose flotation method followed by molecular characterization of the parasites by sequencing pcr products amplified from ssu rrna gene were used. stool samples were collected from domestic animals raised as pets and livestock in all rural properties surrounding three forest f ...201021184703
dna extraction from hair shafts of wild brazilian felids and canids.wild felids and canids are usually the main predators in the food chains where they dwell and are almost invisible to behavior and ecology researchers. due to their grooming behavior, they tend to swallow shed hair, which shows up in the feces. dna found in hair shafts can be used in molecular studies that can unravel, for instance, genetic variability, reproductive mode and family structure, and in some species, it is even possible to estimate migration and dispersion rates in given populations ...201021174262
antibodies to toxoplasma gondii and neospora caninum in captive neotropical and exotic wild canids and felids.this study was designed to detect antibodies to toxoplasma gondii and neospora caninum in wild captive carnivores maintained in brazilian zoos. blood samples were collected from 142 brazilian wild felids and 19 exotic felids in zoos, and 3 european wolves (canis lupus) and 94 brazilian wild canids maintained in captivity in brazilian zoos of são paulo, mato grosso states and federal district. one hundred and two (63.4%) and 70 (50.3%) of the 161 wild felids tested were seropositive for t. gondii ...201020950109
survey of ticks (acari: ixodidae) and their rickettsia in an atlantic rain forest reserve in the state of são paulo, brazil.the current study investigated the occurrence of ticks and their rickettsiae in the serra do mar state park, which encompasses one of the largest atlantic rain forest reserves of brazil. from july 2008 to june 2009, a total of 2439 ticks (2,196 free living and 243 collected on hosts) was collected, encompassing the following 13 species: amblyomma aureolatum (pallas), amblyomma brasiliense aragao, amblyomma dubitatum neumann, amblyomma fuscum neumann, amblyomma incisum neumann, amblyomma longiros ...201020939390
study of sand fly fauna in an endemic area of american cutaneous leishmaniasis and canine visceral leishmaniasis in the municipality of espírito santo do pinhal, são paulo, brazil.canine american visceral leishmaniasis and american cutaneous leishmaniasis (acl) cases have been recorded in espírito santo do pinhal. the aim of this study was to gather knowledge of the sand fly community and its population ecology within the municipality. captures were made weekly over a period of 15 months in the urban, periurban and rural areas of the municipality, using automatic light traps. a total of 5,562 sand flies were collected, comprising 17 species. the most abundant species were ...201020428683
search for mycobacterium leprae in wild mammals.leprosy is still a worldwide public health problem. brazil and india show the highest prevalence rates of the disease. natural infection of armadillos dasypus novemcinctus with mycobacterium leprae has been reported in some regions of the united states. identification of bacilli is difficult, particularly due to its inability to grow in vitro. the use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. in the present study, the diagnostic metho ...201020428654
human toxocariasis: contribution by brazilian researchers.in the present paper the main aspects of the natural history of human infection by toxocara larvae that occasionally result in the occurrence of visceral and/or ocular larva migrans syndrome were reviewed. the contribution by brazilian researchers was emphasized, especially the staff of the tropical medicine institute of são paulo (imt).200920209265
[characteristics of biting animals submitted to rabies diagnosis, são paulo state, brazil, 1993-2007].epidemiological factors related to animal bites in humans and other animals were obtained from a database with 10,616 records of animal specimens sent for rabies diagnosis in northwest são paulo state, brazil, from 1993 to 2007. of this total, 61.5% contained information on the bites, and among the biting animals (25%), the majority were dogs (67%), followed by cats (21.8%), and bats (8.1%). in 92.1% of the reports the victims were humans, and 82.3% of the animals were home pets. the majority of ...200920191165
relationship between dog culling and incidence of human visceral leishmaniasis in an endemic area.domestic dogs are the main reservoirs of visceral leishmaniasis (vl) in urban areas; one of the control measures adopted in brazil is the elimination of this reservoir. in order to test the relationship between the euthanasia rate of the canine reservoir and the incidence of the disease in humans, data on dog culling from the centre for zoonosis control of araçatuba, são paulo, brazil, during the period from 1999 to 2008 and visceral leishmaniasis human cases registered in the same period were a ...201020181428
diminished cd4+/cd25+ t cell and increased ifn-gamma levels occur in dogs vaccinated with leishmune in an endemic area for visceral leishmaniasis.the leishmune vaccine has been used in endemic areas to prevent canine visceral leishmaniasis in brazil, but cytokine production induced by vaccination has rarely been investigated in dogs. this study aimed to evaluate the immune response of dogs vaccinated with leishmune fml vaccine (fort dodge) against total antigen of leishmania (leishmania) chagasi (tag) and fml. twenty healthy dogs from araçatuba, são paulo, brazil, an endemic leishmaniasis area, received three consecutive subcutaneous inje ...201020132994
prevalence of von willebrand disease in dogs from sao paulo state, brazil.the aims of the current study were to assess the prevalence of von willebrand disease (vwd) in dogs from the region of botucatu, são paulo state, brazil, and to evaluate laboratory tests to diagnose this disease. the study included 350 dogs of various ages, different breeds, and both sexes. dogs included in the study had no historical or clinical evidence of abnormal bleeding. von willebrand factor antigen (vwfag), buccal mucosal bleeding time, activated partial thromboplastin time, and factor v ...201020093683
toxoplasma gondii and leishmania spp. infection in captive crab-eating foxes, cerdocyon thous (carnivora, canidae) from brazil.the zoonoses toxoplasmosis and leishmaniasis are important worldwide and also affect wild animals. thus, the present study aimed to assess the prevalence of leishmania spp. and toxoplasma gondii antibodies in 52 serum samples from captive crab-eating foxes (cerdocyon thous) kept in 17 zoos in são paulo state, brazil. modified agglutination test (mat, for toxoplasmosis) and indirect fluorescent antibody test (ifat, for toxoplasmosis and leishmaniasis) were employed with heterologous anti-dog immu ...201020060648
[ehrlichia canis in dogs attended in a veterinary hospital from botucatu, são paulo state, brazil].this study investigated the etiology of canine ehrlichiosis and possible clinical and epidemiological data associated with the infection in 70 dogs suspect of ehrlichiosis attended at the veterinary hospital of the são paulo state university in botucatu city during 2001 and 2002. dogs were evaluated by clinical-epidemiological and hematological data and molecular analysis by partial amplification and dna sequencing of the ehrlichial dsb gene. e. canis dna was amplified and sequenced in 28 (40.0% ...200919772777
cutaneous pythiosis in a dog from brazil.this report describes a case of cutaneous pythiosis in a 6-year-old female mixed breed dog, from the central west region of são paulo state, brazil. the cytological and histopathological analyses showed an intense inflammatory infiltrate with presence of numerous hyphal elements, suggesting infection due to pythium insidiosum. the diagnosis was confirmed by nested-pcr, which was carried out with specific primers derived from the ribosomal dna region. the pathogen occurs in brazil and veterinaria ...201019706006
babesia spp. infection in dogs from rural areas of são paulo state, brazil.the status of babesia spp. infection in dogs from rural areas of são paulo state, brazil was studied. for this, 150 animals were examined by blood smears and by pcr; the presence of tick infestation was also investigated. by the blood smear examination, 3 animals (2%) were detected positive and by pcr for babesia spp. 12 (8%) were positive, with bands visualized in 450 bp. rhipicephalus sanguineus or amblyomma spp. were found on 36 (24%) of the 150 dogs. amblyomma species found were a. cajennens ...200919602312
[upgrading course for animal control agents: a new perspective on the zoonosis control services in the state of são paulo, southeastern brazil]. 200919448918
evaluation of dna damage by the alkaline comet assay of the olfactory and respiratory epithelia of dogs from the city of são paulo, brazil.animals kept as pets may be considered sentinels for environmental factors to which humans could be exposed. olfactory and respiratory epithelia are directly subjected to airborne factors, which could cause dna lesions, and the alkaline comet assay is considered a reliable tool for the assessment of dna damage. the objective of this work is to evaluate the extent of dna damage by the comet assay of the olfactory and respiratory epithelia of dogs from different regions of the city of são paulo, b ...201019447591
tesa-blot for the diagnosis of chagas disease in dogs from co-endemic regions for trypanosoma cruzi, trypanosoma evansi and leishmania chagasi.we standardized serodiagnosis of dogs infected with trypanosoma cruzi using tesa (trypomastigote excreted-secreted antigen)-blot developed for human chagas disease. tesa-blot showed 100% sensitivity and specificity. in contrast, elisa using tesa (tesa-elisa) or epimastigotes (epi-elisa) as antigen yielded 100% sensitivity but specificity of 94.1% and 49.4%, respectively. when used in field studies in an endemic region for chagas disease, visceral leishmaniasis and trypanosoma evansi (mato grosso ...200919426657
evidence of leishmania spp. antibodies and dna in bush dogs (speothos venaticus) in brazil.the municipality of ilha solteira, são paulo, brazil is an endemic area of leishmaniasis. at the companhia energética de são paulo (cesp) wild animal center of ilha solteira, two bush dogs (speothos venaticus) showed clinical signs of this disease. the amastigote form of leishmania was detected in lymph-node smears taken by fine-needle biopsy. in addition, serum samples from both animals, screened with an enzyme-linked immunosorbent assay (elisa), were positive for anti-leishmania antibodies. mo ...200919368245
toxocariasis/cysticercosis seroprevalence in a long-term rural settlement, são paulo, brazil.seroprevalence of toxocara and taenia solium and risk factors for infection with these parasites were explored in a long-term rural settlement in são paulo state, brazil. an elisa for the detection of anti-toxocara igg and ige and anti-t. solium cysticerci was standardized using toxocara excretory-secretory antigens (tes) obtained from the cultured second-stage larvae of t. canis and by vesicular fluid antigen from taenia crassiceps cysticerci (vf). for cysticercosis, the reactive elisa samples ...200919366477
[epidemiological profile of rabies in the northwestern region of são paulo state, from 1993 to 2007].the epidemiology of animal rabies in the region of araçatuba, in the northwest of são paulo state, from 1993 to 2007, is described according to the results from diagnoses made at laboratories in the region, using the fluorescent antibody and mouse inoculation tests. out of 10,579 samples analyzed, 4.9% were positive (518/10,579). dogs accounted for 67% of the cases (346/518) and these occurred between 1993 and 1997. among the other positive samples, 16% (84/518) were in cattle and 9.7% (50/518) ...200919287928
classic and molecular study of giardia duodenalis in children from a daycare center in the region of presidente prudente, são paulo, brazil.epidemiological studies on giardiasis by using molecular techniques such as rapd (randomly amplified polymorphic dna) may give information on factors related to the transmission of giardia duodenalis. the aim of this work was to assess the epidemiology of g. duodenalis in 101 children attended at a daycare center in presidente bernardes, sp, brazil. after parasitological examinations in feces samples, 15 children presented cysts of g. duodenalis. their respective parents, brothers and pets, besi ...200919229386
domestic cats constitute a natural reservoir of human enteropathogenic escherichia coli types.feces of 70 diarrhoeic and 230 non-diarrhoeic domestic cats from sao paulo, brazil were investigated for enteropathogenic (epec), enterohaemorrhagic (ehec) and enterotoxigenic (etec) escherichia coli types. while etec and ehec strains were not found, 15 epec strains were isolated from 14 cats, of which 13 were non-diarrhoeic, and one diarrhoeic. none of 15 epec strains carried the bfpa gene or the epec adherence factor plasmid, indicating atypical epec types. the epec strains were heterogeneous ...200919068073
use of geographic information systems in rabies vaccination campaigns.to develop a method to assist in the design and assessment of animal rabies control campaigns.200819009158
environmental contamination by toxocara spp. eggs in a rural settlement in brazil.in order to study the environmental contamination by toxocara spp. eggs in a rural community from the pontal do paranapanema region, são paulo state, brazil, soil samples from 31 out of 121 plots were collected in eight different places on each house. the samples were submitted to flotation technique in sodium nitrate (d = 1.20 g/cm3). eggs of toxocara spp. were recovered in nine (29.03%) out of the 31 plots. at least one dog was registered in 27 of the 31 plots examined (87.1%) and at least one ...200818949345
prevalence of dog intestinal parasites and risk perception of zoonotic infection by dog owners in são paulo state, brazil.coprological examination was used to estimate the prevalence of gastrointestinal parasites in stray and domiciled dogs from botucatu, são paulo state, brazil. risk factors for dog infection were assessed in relation to demographic, husbandry and management data. the dog owners completed a questionnaire survey on some aspects of dog parasitism such as parasite species, mechanisms of infection, awareness of zoonotic diseases and history of anthelmintic usage. parasites were found in the faeces of ...200818811905
[occurrence of antibodies anti-neospora caninum in dogs of botucatu range micro region, state of são paulo, brazil].neosporosis is a parasitic disease caused by a protozoon neospora caninum, which is recognized as an important cause of bovine abortion and canine neuropathy. considering the isolation of n. caninum and the frequent serology in bovines of our region, the objectives of the present trial were to evaluate the occurrence of antibodies anti-n. caninum in dogs of the botucatu range micro region, in the state of são paulo, and its association with gender, age and origin (urban area--exclusively from th ...200818554432
antigenic and genetic characterization of the first rabies virus isolated from the bat eumops perotis in brazil.although the main transmitters of rabies in brazil are dogs and vampire bats, the role of other species such as insectivorous and frugivorous bats deserves special attention, as the rabies virus has been isolated from 36 bat species. this study describes the first isolation of the rabies virus from the insectivorous bat eumops perotis. the infected animal was found in the city of ribeirão preto, são paulo. the virus was identified by immunofluorescence antibody test (fat) in central nervous syst ...200818488088
genetic and antigenic diversities of major immunoreactive proteins in globally distributed ehrlichia canis strains.the extent of knowledge regarding the diversity of globally distributed ehrlichia canis strains has been limited to information gained from a few evolutionarily conserved genes. in this study, e. canis strains from the united states (strain jake [us]), brazil (strain são paulo [br]), and israel (strain 611 [is] and ranana [is-r]) were used to examine the antigenic and genetic diversities of four well-characterized major immunoreactive protein genes/proteins. gp36 and gp200 were the most divergen ...200818480237
[a descriptive profile of the canine population in araçatuba, são paulo state, brazil, from 1994 to 2004].from 1994 to 2004, the canine population in araçatuba, são paulo state, brazil, suffered two major canine zoonoses: rabies and visceral leishmaniasis. changes in the dog population during this period were evaluated using canine census data from 1994 and 2004 and the results of blood samples for diagnosis of canine visceral leishmaniasis in 1999. the ratio of dogs per 10 inhabitants varied from 1.7 in 1994 to 2.0 in 1999 and 1.8 in 2004. the percentage of puppies less than 1 year of age increased ...200818392371
serosurvey of rickettsia spp. in dogs and humans from an endemic area for brazilian spotted fever in the state of são paulo, brazil.the present study provides a rickettsial serosurvey in 25 dogs and 35 humans in an endemic area for brazilian spotted fever in the state of são paulo, where the tick amblyomma aureolatum is the main vector. testing canine and human sera by indirect immunofluorescence against four rickettsia antigens (r. rickettsii, r. parkeri, r. felis and r. bellii) showed that 16 (64%) of canine sera and 1 (2.8%) of human sera reacted to at least one of these rickettsial antigens with titers >0r= 64. seven can ...200818278271
molecular and parasitological survey of hepatozoon canis (apicomplexa: hepatozoidae) in dogs from rural area of sao paulo state, brazil.hepatozoon canis is a protozoan that infects dogs and is transmitted by the ingestion of the brown dog tick, rhipicephalus sanguineus. two distinct species of hepatozoon genus can infect dogs, h. canis and h. americanum. routine tests to detect the disease are based on direct examination of gametocytes on giemsa-stained blood smears. the objectives of this study were the investigation of infection prevalence in rural area dogs, the comparison of diagnostics by blood smear examination and polymer ...200818188597
human asymptomatic infection in visceral leishmaniasis: a seroprevalence study in an urban area of low endemicity. preliminary results.many aspects of the human asymptomatic visceral leishmaniasis (vl) remain not elucidated, and moreover, almost all the data come from highly endemic areas. the recent appearance of american vl (avl) in the northeastern region of the state of são paulo, brazil, offered a good opportunity for further understanding. we present the preliminary results from a seroprevalence study on avl in humans in araçatuba, são paulo. this was a cross-sectional survey on a random sample of the population (one-stag ...200718165520
a fibrin-related line of research and theoretical possibilities for the use of fibrin glue as a temporary basal membrane in non-perforated corneal ulcers and in photorefractive keratectomy (prk)-operated corneas.to report a specific line of research developed at the university of são paulo/brazil on fibrin glue used for sealing corneal perforations and the perspectives of use on non-perforated corneal ulcers and photorefractive keratectomy-operated corneas.200718157320
rickettsia infection in five areas of the state of são paulo, brazil.this study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of são paulo. eight flea species (adoratopsylla antiquorum antiquorum, ctenocephalides felis felis, polygenis atopus, polygenis rimatus, polygenis roberti roberti, polygenis tripus, rhopalopsyllus lugubris, and rhopalopsyllus lutzi lutzi), and five tick species (amblyomma aureolatum, amblyomma cajennense, amblyomma dubitatum, ixodes loricatus, and rhipicephalus sanguineus) were ...200718094887
surveillance for zoonotic vector-borne infections using sick dogs from southeastern brazil.for many vector-borne organisms, dogs can be used as sentinels to estimate the risk of human infection. the objective of this study was to use dogs as sentinels for multiple vector-borne organisms in order to evaluate the potential for human infection with these agents in southeastern brazil. blood from 198 sick dogs with clinicopathological abnormalities consistent with tick-borne infections were selected at the são paulo state university veterinary teaching hospital in botucatu and tested for ...200718021025
molecular identification of cryptosporidium spp. from fecal samples of felines, canines and bovines in the state of são paulo, brazil.the objective of this study was to obtain information of epidemiological nature through genotypic characterization of cryptosporidium isolates from dogs, cats and bovines from the state of são paulo, brazil. the extraction of dna from oocysts was carried out and polymerase chain reaction was accomplished using specific primers to 18s rrna gene. the amplicons were directed sequenced. seven cat samples, nine dog samples and nine bovine samples were analysed. from the seven cat samples the genotypi ...200717996373
human and canine leptospirosis: serological data of são paulo city, brazil, 2000 to 2003.sera from humans and dogs were tested by microscopic agglutination test. icterohaemorrhagiae was the predominant serogroup in both groups, associated with the infestation of rattus norvegicus.200517966484
population structure and mouse-virulence of toxoplasma gondii in brazil.recent studies found that isolates of toxoplasma gondii from brazil were biologically and genetically different from those in north america and europe. however, to date only a small number of isolates have been analysed from different animal hosts in brazil. in the present study dna samples of 46 t. gondii isolates from cats in 11 counties in são paulo state, brazil were genetically characterised using 10 pcr restriction fragment length polymorphism markers including sag1, sag2, sag3, btub, gra6 ...200817963770
molecular identification of giardia duodenalis isolates from humans, dogs, cats and cattle from the state of são paulo, brazil, by sequence analysis of fragments of glutamate dehydrogenase (gdh) coding gene.the nucleotide sequence of glutamate dehydrogenase (gdh) coding genes were obtained from cysts of giardia duodenalis isolated from feces of naturally infected cats (n=19), dogs (n=27), humans (n=37) and cattle (n=5). the samples were from several municipalities within the state of são paulo, brazil and were collected from january 2004 to august 2006. sequences analysis of the 37 specimens recovered from humans revealed 29 g. duodenalis assemblage aii and 8 g. duodenalis assemblage b. among sampl ...200717900812
serological survey of leishmania infantum and trypanosoma cruzi in dogs from urban areas of brazil and colombia.leishmania infantum and trypanosoma cruzi are zoonotic parasites that are endemic throughout many parts of latin america. infected dogs play an important role in transmission of both parasites to humans. a serological survey of leishmania and trypanosoma infection was conducted on 365 dogs from são paulo, brazil and bogatá, colombia, south america. serum samples were examined by the indirect immunofluorescent antibody test (ifat). anti-leishmania igg antibodies were detected in 5 of 107 from bra ...200717825991
the first records of leishmania (leishmania) amazonensis in dogs (canis familiaris) diagnosed clinically as having canine visceral leishmaniasis from araçatuba county, são paulo state, brazil.two cases of leishmania (leishmania) amazonensis are reported in the domestic dog (canis familiaris). these are the first records of this parasite in this species. the animals lived in the endemic visceral leishmaniasis area of araçatuba, são paulo state, brazil and were initially diagnosed, on clinical grounds, as having visceral leishmaniasis. attempted parasite isolation from inguinal lymph node aspirates was unsuccessful and the indirect immunofluorescent test for visceral leishmaniasis was ...200717720321
canine bartonellosis: serological and molecular prevalence in brazil and evidence of co-infection with bartonella henselae and bartonella vinsonii subsp. berkhoffii.the purpose of this study was to determine the serological and molecular prevalence of bartonella spp. infection in a sick dog population from brazil. at the são paulo state university veterinary teaching hospital in botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. antibodies to bartonella henselae and bartonella vinsonii subsp. berkhoffii were detected in 2.0% (4/197) and 1.5% (3/197) of the dogs, respectively. using 16s-2 ...200717583666
diverse and atypical genotypes identified in toxoplasma gondii from dogs in são paulo, brazil.the prevalence of toxoplasma gondii in 118 unwanted dogs from são paulo city, são paulo state, brazil, was determined. antibodies to t. gondii were assayed by the modified agglutination test and found in 42 (35.8%) dogs, with titers of 1:20 in 10, 1:40 in 6, 1:80 in 5, 1:160 in 5, 1:320 in 6, 1:640 in 7, and 1:1,280 or higher in 3. hearts and brains of 36 seropositive dogs were bioassayed in mice, or cats, or both. tissues from 20 seropositive dogs were fed to 20 t. gondii-free cats. feces of ca ...200717436942
prevalence of ehrlichia canis (rickettsiales: anaplasmataceae) in dogs and rhipicephalus sanguineus (acari: ixodidae) ticks from brazil.the current study evaluated the prevalence of ehrlichia canis donatien and lestoquard in domestic dogs, canis familiaris l., and rhipicephalus sanguineus (latreille) (acari: ixodidae) ticks from different areas of brazil. in monte negro county (state of rondônia, brazilian western amazon), the indirect immunofluorescence assay detected e. canis-reactive antibodies (titer > or = 40) in 58/153 (37.9%) and 40/161 (24.8%) dogs from the urban and rural areas, respectively. these values were significa ...200717294930
frequency of soil contamination by toxocara canis eggs in the south region of são paulo municipality (sp, brazil) in a 18 month period.soil contamination by embryonic eggs of toxocara canis is the main source of human infection by this ascarid larvae resulting, sometimes, in the occurrence of visceral larva migrans syndrome. the objective of the present research is to determine the frequency of t. canis eggs in soil samples monthly collected in nine public places, located at the south region of são paulo municipality in a 18-month period, from february 2004 to july 2005. the soil samples collected were treated with a 30% antifo ...200617221127
isolation of rickettsia rickettsii and rickettsia bellii in cell culture from the tick amblyomma aureolatum in brazil.brazilian spotted fever (bsf) is a highly lethal disease caused by rickettsia rickettsii. in the present study, rickettsial infection was evaluated in 669 amblyomma aureolatum adult ticks collected from naturally infested dogs in taiaçupeba, a bsf-endemic area in the state of são paulo. ten (1.49%) ticks were infected with rickettsia bellii, and 6 (0.89%) ticks were infected with r. rickettsii. both rickettsia species were isolated and established in vero cell cultures. the rickettsia isolates w ...200617114770
prevalence of rickettsia felis in the fleas ctenocephalides felis felis and ctenocephalides canis from two indian villages in sao paulo municipality, brazil.we evaluated the presence of rickettsia infection among fleas collected on domestic dogs in two guarani indian communities in the suburban area of são paulo municipality, brazil. a total of 114 ctenocephalides felis felis and 47 ctenocephalides canis were collected from 40 dogs. a total of 41 c. felis felis (36.0%) and 9 c. canis (19.1%) fleas yielded expected bands by pcr, which were all shown by dna sequencing to be indentical to the corresponding sequence of a fragment of the rickettsia felis ...200617114741
a preliminary investigation of ehrlichia species in ticks, humans, dogs, and capybaras from brazil.a molecular epidemiologic investigation in two brazilian states (rondônia and são paulo) was undertaken to determine if ehrlichia species responsible for human and animal ehrlichioses in north america could be found in brazilian vectors, potential natural mammalian reservoirs and febrile human patients with a tick bite history. samples, including 376 ticks comprising 9 amblyomma species, 29 capybara (hydrochaeris hydrochaeris) spleens, 5 canine blood, and 75 human blood samples from febrile pati ...200716962245
seroprevalence of toxoplasmosis in a low-income community in the são paulo municipality, sp, brazil.toxoplasmosis is one of the most common zoonoses worldwide. the seroprevalence for t. gondii in human population from brazil might range from 40 to 80%. the aim of this paper was to study the seroprevalence of t. gondii infection in children from age one to 15 living in a low socioeconomic community, named community of jardim são remo in the year of 2002. the community is located in the west area of são paulo municipality, são paulo state, brazil. antibodies to t. gondii were found in 110 (32.4% ...200616847507
[prevalence of cryptosporidium sp. in institutionalized dogs in the city of são paulo, brazil].dogs play an important role as infection source of human cryptosporidiosis. the objective of the study was to determine the prevalence of cryptosporidium sp. in dogs as well as to compare two techniques of fecal analysis.200616410992
[laboratory diagnosis of rabies in the west region of são paulo state].the pólo da alta sorocabana laboratory, presidente prudente, sp, brazil, and the biological institute in são paulo state, performed an evaluation of rabies diagnosis from 1996 to 2003 in the west region of são paulo state. for the tests, the laboratories used direct immunofluorescence and mice inoculation in 4,950 samples, that were sent for analysis involving dogs, cats, cattle, chiroptera (bats) and other animals. according to the results, the laboratories found 74 positive samples; of which 5 ...200616410925
[estimation of the dog and cat population in the state of são paulo].to estimate the total dog and cat population with owners in order to enable better planning of the control actions against diseases involving these animals.200516341397
adoption of shelter dogs in a brazilian community: assessing the caretaker profile.a survey in ibiúna, sao paulo, brazil, of caregivers (owners) who adopted shelter dogs assessed length of ownership, proportion of male and female dogs adopted, and owners' characteristics. it addressed breeding, neutering, vaccination, and veterinary care. it used no testing to provide a good "match" between dog and future owner. of adopted dogs, 58% were male. only 36% of owners were located. mean ownership length was 14.8 months (95% confidence interval = 12.4 to 17.2 months), estimated throu ...200516277594
environmental contamination by toxocara sp. eggs in ribeirão preto, são paulo state, brazil.toxocariasis is a zoonosis mainly caused by toxocara canis, an intestinal nematode of dogs. man acquires the infection through accidental ingestion of viable eggs, and the toxocariasis clinical manifestations may vary from an asymptomatic infection up to the visceral larva migrans syndrome. seventy eight public squares of ribeirão preto, são paulo, brazil, including bonfim paulista district were visited aiming to evaluate the soil contamination by toxocara eggs. the squares were divided in five ...200516138206
epidemiological aspects of visceral larva migrans in children living at são remo community, são paulo (sp), brazil.in preparation for a study of risk factors for visceral larva migrans of children living in são remo community, são paulo (sp), brazil, fecal samples were collected from 41 dogs younger than one year of age, 338 serum samples from children ages 1-15 years and 37 soil samples, during march-june 2001. fecal and soil samples were examined for the presence of toxocara spp. eggs and serum samples were evaluated by elisa for antibodies to toxocara canis. of the fecal, serum and soil samples tested, 39 ...200516054301
[contamination of public gardens along seafront of praia grande city, são paulo, brazil, by eggs of ancylostoma and toxocara in dogs feces].contamination of the lawns and flower beds along the seafront of praia grande, sp, brazil, by eggs of ancylostoma and toxocara in fecal samples of dogs was evaluated. of the total samples analyzed, 45.9% were contaminated with ancylostoma eggs and 1.2% with toxocara eggs.200515821802
rickettsial infection in animals and brazilian spotted fever endemicity.we compared the rickettsial infection status of amblyomma cajennense ticks, humans, dogs, and horses in both brazilian spotted fever (bsf)-endemic and -nonendemic areas in the state of sao paulo, brazil. most of the horses and few dogs from bsf-endemic areas had serologic titers against rickettsia rickettsii antigens. in contrast, no dogs or horses from bsf-nonendemic areas had serologic titers against r. rickettsii antigens, although they were continually exposed to a. cajennense ticks. all hum ...200515752445
[american leishmaniasis in the state of são paulo: epidemiological status in 2001-2002]. 200315719771
[infected lutzomyia edwardsi found in the greater sao paulo area]. 200515654471
human toxocariasis: a seroepidemiological survey in schoolchildren of sorocaba, brazil.a seroepidemiological survey for toxocariasis, among 180 schoolchildren of the public schools of sorocaba city, state of são paulo, brazil, was carried out from august 2000 to july 2001. elisa test was performed using excretory and secretory antigens for the detection of igg anti-toxocara antibodies. information regarding the children was obtained from the parents or legal guardians. the results showed that the mean age was 5.4 +/- 1.4 years, the infection coefficient (ic) was 38.3 and the infec ...200415558160
tissue stages of hepatozoon canis in naturally infected dogs from sao paulo state, brazil.a total of 222 dogs were examined by blood smear examination and hepatozoon canis infection was detected in 13 dogs (5.9%). five h. canis-infected dogs were necropsied to observe tissue stages in the organs. fragments of spleen, liver, lungs, heart, kidneys, lymph nodes, bone marrow and skeletal muscles were used to made touch-impression smears. no macroscopic lesions were found in the organs. two dogs had gamonts within polymorphonuclear cells and schizonts in various stages of development with ...200415338284
prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies in ovine from são paulo state, brazil.serum samples from 597 sheep from são paulo state, in the southeastern region of brazil, were tested to determine the prevalence of antibodies directed against toxoplasma gondii (> or = 1:64) and neospora caninum (> or = 1:50) using the indirect fluorescent antibody test (ifat). the animals were divided into three groups based on their age: < or = 1 year, 1-4 years, and > or = 4 years. antibodies to t. gondii were observed in 34.7% of the samples with titers ranging from 64 to 16,384 and igg ant ...200415325042
[estimate of the owned canine and feline populations in urban area in brazil].given the importance of assessing owned dog and cat populations to adequate planning and evaluation of rabies control measures in urban areas, it is proposed the use of an estimate of these populations based on human population parameters.200415311298
attaching and effacing escherichia coli isolated from dogs in brazil: characteristics and serotypic relationship to human enteropathogenic e. coli (epec).escherichia coli isolates recovered from 182 fecal specimens from dogs up to five months old from the cities of são paulo and campinas, sp, brazil, were examined by polymerase chain reaction (pcr) for several virulence factors and properties. the eae gene was found in 23 isolates of e. coli from 22 dogs, 19 of 146 (13%) from dogs with diarrhea and 3 of 36 (8.3%) from dogs with no diarrhea. two different eae+ isolates were recovered from one dog with diarrhea. isolates from two dogs with diarrhea ...200415262000
prevalence of antibodies to spotted fever group rickettsiae in humans and domestic animals in a brazilian spotted fever-endemic area in the state of são paulo, brazil: serologic evidence for infection by rickettsia rickettsii and another spotted fever group rickettsia.in serum samples obtained from all the healthy humans, horses, dogs, and donkeys present on three farms in the pedreira municipality, an endemic area for brazilian spotted fever, an indirect immunofluorescence assay (ifa) detected antibodies against rickettsia rickettsii in 17 (77.3%) horses, 5 (31.3%) dogs (titers ranging from 64 to 4,048), and none of 4 donkeys or 50 humans. five canine and eight equine sera with high antibody titers to r. rickettsii were also tested by ifa against r. bellii, ...200415238696
study of the seasonal dynamics, life cycle, and host specificity of amblyomma aureolatum (acari: ixodidae).in the first part of this study, monthly infestation by ticks was evaluated on dogs from december 2000 to november 2002 in the rural area of taiaçupeba, são paulo. adults of amblyomma aureolatum (pallas) were found on dogs in all months, with a mean prevalence per month of 46.9 +/- 15.7% (range, 25-80%). the mean tick relative abundance per month was 2.4 +/- 2.7 ticks (range, 0.5-14 ticks), and the mean tick mean intensity per month was 4.7 +/- 4.2 ticks (range, 1.5-23.3 ticks). no a. aureolatum ...200415185932
canine rabies epidemiology in araçatuba and neighborhood, northwestern são paulo state-brazil.epidemiological characteristics of canine rabies in the northwest region of são paulo state (araçatuba region), brazil, from 1993 to 1997 are presented. out of 1,984 dogs, a total of 351 (17.7%) were positive for rabies diagnosis; 89% (312/351) of these occurred in urban areas and 85% (266/312) of the urban positive cases were among owned dogs. the mean age of the rabid dogs was 34 months and 61% were male. aggressive behavior was observed in 77% of rabid dogs, followed by lack of coordination a ...200415094898
host-feeding patterns of potential human disease vectors in the paraíba valley region, state of säo paulo, brazil.engorged females of aedes albopictus, ochlerotatus scapularis, culex nigripalpus and culex quinquefasciatus were collected by aspiration and sweep net during two years in tremembé county, state of säo paulo, brazil. of the 1,092 specimens analyzed with the precipitin test, 87.6% reacted to one or more of the eight antisera tested. of the four species for which the host determination was made, the reaction in 98.5% was to a single host. the application of the feeding index for four species of mos ...200312831131
ticks (acari: ixodidae) associated with domestic dogs in franca region, são paulo, brazil.ticks on 140 domestic dogs from both urban and rural areas of franca region in são paulo state were identified with 102 dogs from urban areas and 38 from rural areas. of urban dogs, 27.5% were infested exclusively by rhipicephalus sanguineus ticks. of the rural dogs, 36.8% were infested with the following tick species: r. sanguineus, boophilus microplus, amblyomma ovale and a. cajennense. mixed infestations included a dog hosting a. cajennense and a. ovale and another with b. microplus and r. sa ...200112455880
daycare centers and schools as sources of exposure to mites, cockroach, and endotoxin in the city of são paulo, brazil.public places, including schools, have been identified as sources of exposure to allergens derived from mites, cockroach, cat, and dog and to endotoxin.200212373265
[são paulo pasteur institute technique for estimating a canine population].this paper describes a technique developed by the pasteur institute in são paulo, brazil, for counting and classifying a canine population according to levels of dependency and confinement. first, a household survey was performed to identify dogs with owners and their degree of restriction. different-colored collars were given to totally and partially restricted dogs. dogs cared for by individuals who did not consider themselves the owners received collars of a third color. after the survey, sur ...200212244375
gynandromorphism in amblyomma cajennense and rhipicephalus sanguineus (acari: ixodidae).during a survey of ticks on horses in november 2000, at the university of são paulo farm in pirassununga county, são paulo, brazil, 1 gynandromorph of amblyomma cajennense was collected from a naturally infested horse. in another survey on dogs in march 2001, in the urban area of monte negro, rondônia, brazil, a gynandromorph of rhipicephalus sanguineus was collected from a naturally infested dog. both specimens are described and classified as perfect bipartite protogynanders.200212197141
occurrence of neospora caninum antibodies in sera from dogs of the city of são paulo, brazil.neospora caninum is an important cause of abortion in dairy cattle worldwide. dogs are important in the epidemiology of this parasite because they are the only hosts known to excrete n. caninum oocysts. in order to understand the prevalence of n. caninum in dogs, sera from 500 owned dogs and from over 600 feral street dogs from the city of são paulo, brazil were assayed for antibodies to n. caninum. sera were examined by the neospora agglutination test (nat) using mouse-derived tachyzoites. anti ...200212031819
epidemiological and serological aspects in canine toxoplasmosis in animals with nervous symptoms.the presence of anti-toxoplasma gondii igm and igg antibodies was studied in samples of blood serum taken from eighty dogs with nervous symptoms at the serviço de enfermidades infecciosas dos animais, faculdade de medicina veterinária e zootecnia, unesp, botucatu, são paulo, brazil. the frequency of igg titers were 16 (13.7%), 64 (13.7%), and 256 (5%), and for igm titers were 16 (7.5%), 64 (15%), and 256 (8.7%). positive reactions were more frequent in the older animals, males, from a rural envi ...200211992143
common causes of poisoning in dogs and cats in a brazilian veterinary teaching hospital from 1998 to 2000.a retrospective study at the university veterinary teaching hospital of são paulo city, brazil, describes the pattern of poisoning that occurred from 1998 to 2000. during this period 5,136 animals were sent to the emergency sector, and 250 of these cases were reported as poisonings: 203 dogs (81.2%) and 47 cats (18.8%). the common causes of poisoning in dogs were 28.9% therapeutic products (86.4% non-steroidal anti-inflammatory drugs, 6.8% other drugs, 3.4% antibiotics, 3.4% tranquilizing agents ...200211931501
[detection of cyclospora sp oocysts in the feces of stray dogs in greater são paulo (são paulo state, brazil)].fecal samples from 140 adult stray dogs of greater são paulo (são paulo state, brazil) were examined for cyclospora sp oocysts. no cases of infection by this coccidium were detected.200111813070
prevalence of intestinal parasites in dogs from são paulo state, brazil.the prevalence of gastrointestinal parasites in stray dogs, and dogs with owners was investigated by fecal examinations from 271 dogs employing sedimentation, simple flotation and centrifugation-flotation methods. the centrifugation-flotation method, when compared to simple flotation or sedimentation methods was generally more accurate in the diagnosis of all intestinal parasites, but statistical differences were detected only in relation to giardia spp. and cystoisospora spp. (synonym isospora ...200211750997
borrelia burgdorferi antibodies in dogs from cotia county, são paulo state, brazil.dogs sera samples collected from cotia county, são paulo were tested using indirect immunoenzymatic test (elisa) in order to study lyme disease serology in dogs. elisa method was standardized and g39/40 north american strain of borrelia burgdorferi was used as antigen. positive results were confirmed employing the western blotting technique. because of the possibility of cross-reactions, sera were also tested for different serological strains of leptospira interrogans and l. biflexa using micros ...200111696846
[use of spatial analysis tools in the epidemiological surveillance of american visceral leishmaniasis, araçatuba, são paulo, brazil, 1998-1999].the control of american visceral leishmaniasis (avl) is based on combating the vector and eliminating the domestic reservoir of the focus area - defined as 200 meters around human or canine cases. this paper discusses the use of spatial analysis techniques in the epidemiological surveillance of avl in araçatuba, são paulo state, in order to propose a model for territorial epidemiological surveillance, reformulating current control strategies. the results showed that avl transmission was not homo ...200111679900
toxocara spp. eggs in public squares of sorocaba, são paulo state, brazil.the visceral larva migrans (vlm) is a syndrome observed in human infection with helminth larval eggs such as the toxocara spp. that usually infects dogs and cats. among the risk factors involved in the occurrence of vlm, particularly important is the size of these animal populations. sorocaba is a city with a dog population twice as large as that recommended by the world health organization. this fact has led to a survey of the presence of toxocara spp. eggs in public square soils of this city. ...200111557996
rabies seroprevalence of street dogs from são paulo city, brazil.a sample of 602 street dogs captured by the são paulo zoonosis-control municipal service was analyzed for the presence of serum antibodies against the rabies virus and compared to a control sample of 37 dogs from the kennel of the police of são paulo. data were stratified into 4 age intervals and the age from the street-dog sample was estimated by general aspects and dental observation according to standard techniques. we assumed that seropositivity was due to previous vaccination (no sub-clinic ...200010760397
[serological survey for american cutaneous leishmaniasis in stray dogs in the s. paulo state, brazil].a serological survey was made in 973 stray dogs caught near green areas in s. paulo county by the indirect immunofluorescence test. no positive serum was found, however autochthonous human cases of acl that occurred in the county show the circulation of the parasite in the environment. this fact, associated with the existence of the vectors and the apparent absence of infected dogs in the areas studied, suggest that stray dog plays an insignificant role in the spread of the parasite. the cycle o ...199910689382
[analysis of human anti-rabies post exposure treatment in the great area of sao paulo, brazil].the treatment of human beings with risk of exposure to rabies virus infection still presents some cases of undesirable post treatment reactions. the knowledge resulting from the regular analysis of the epidemiological features associated with these practices would minimize the occurrence of these troubles.199910457003
[rabies epizootic in the urban area of ribeirão preto, são paulo, brazil].this report describes some epidemiological aspects of a rabies epizootic that started in 1995 in the urban area of ribeirão preto, sp, brazil, and discusses its main causes. all laboratory confirmed cases were described according to a set of epidemiological variables. simultaneously, information was raised concerning rabies vaccine coverage and epidemiological surveillance activities. in addition to one human case, 58 rabid animals were confirmed in 1995 (54 dogs, 3 cats. and 1 bat). there were ...19989878906
the first two cases of cyclospora in dogs, são paulo, brazil.cyclospora cayetanensis is a coccidium which has been identified in diarrheal feces or immunocompetent individuals or in aids patients. the main aspects related to its epidemiology and pathogeny have not been solved so far, as well as the possibility of human infection by this protozoan being a zoonosis. we describe in this work the first case identifying the cyclospora in dogs. our findings, notwithstanding their preliminary characteristics, could suggest that besides untreated water, other tra ...19979460261
epidemiological aspects of the brazilian spotted fever: serological survey of dogs and horses in an endemic area in the state of são paulo, brazil.in order to obtain information on brazilian spotted fever, a study in domestic animals was performed in the county of pedreira, state of são paulo, brazil, where 17 human cases had been notified. serum samples obtained from animals were tested by indirect immunofluorescence for detectable antibodies to spotted fever-group rickettsiae. seropositivity was revealed in 12 (36.4%) of 33 dogs and seven (77.8%) of nine horses from the endemic area. for comparison, blood samples from dogs and horses fro ...19969293089
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