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rt-pcr and cell culture infectivity assay to detect enteroviruses during drinking water treatment processes.in this study, 62 water samples were collected from two water treatment plants (wtps) in suez canal cities (port said and ismaillia) and one plant in cairo (giza wtp) in addition to the beginning of the two nile river branches (rosetta and damietta). viruses were concentrated by adsorption-elution ethod sing 142 mm-diameter nitrocellulose membrane of 0.45 microm pore size and eluted with 3% beef extract at ph 9.5. the concentrated samples were inoculated for 3 successive passages in three cell c ...199917219867
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