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detection of anaplasma bovis and anaplasma phagocytophilum dna from haemaphysalis megaspinosa in hokkaido, japan.dna from 101 ticks, including haemaphysalis megaspinosa, haemaphysalis douglasi, haemaphysalis flava, ixodes ovatus and ixodes persulcatus, collected by flagging in a pasture in hidaka district (hokkaido, japan) was examined for infection with anaplasma bovis and a. phagocytophilum by species-specific nested pcr. of the 48 h. megaspinosa nymphs, examined, 1 (2.1%) and 6 (12.5%) were positive for a. bovis and a. phagocytophilum, respectively. one of the 6 positive nymphs was dual positive for bot ...201019897306
detection of dna of 'candidatus mycoplasma haemominutum' and spiroplasma sp. in unfed ticks collected from vegetation in japan.dna fragments of 'candidatus mycoplasma haemominutum', a feline heamobartonella pathogen, were detected from unfed ixodes ovatus collected from vegetation in hokkaido, fukushima and yamaguchi prefectures, and unfed haemaphysalis flava in yamaguchi prefecture. this finding suggests that ixodid tick is a possible vector of 'c. mycoplasma haemominutum'. spiroplasma dna was also detected from unfed i. ovatus in hokkaido, fukushima and yamaguchi prefectures. the analysis of nucleotides sequence sugge ...200516397391
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