Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| activities of abt-773 against listeria monocytogenes and coryneform bacteria of clinical interest. | the in vitro activities of abt-773 were evaluated against 15 listeria monocytogenes strains and 196 coryneform bacteria isolated from clinical samples. one hundred percent of the l. monocytogenes strains were inhibited by </=0.015 micro g of abt-773/ml. mics of abt-773 ( micro g/ml) at which 50% of the isolates tested were inhibited (mic(50)s) and mic(90)s for other organisms were 0.125 and 0.5 (corynebacterium amycolatum), 1 and >32 (corynebacterium jeikeium), 0.03 and >32 (corynebacterium minu ... | 2003 | 12654678 |
| quantitative, multiplexed detection of bacterial pathogens: dna and protein applications of the luminex labmap system. | escherichia coli, salmonella, listeria monocytogenes and campylobacter jejuni are bacterial pathogens commonly implicated in foodborne illnesses. generally used detection methods (i.e., culture, biochemical testing, elisa and nucleic acid amplification) can be laborious, time-consuming and require multiple tests to detect all of the pathogens. our objective was to develop rapid assays to simultaneously detect these four organisms through the presence of antigen or dna using the luminex labmap sy ... | 2003 | 12654495 |
| detection of bacterial pathogens in environmental samples using dna microarrays. | polymerase chain reaction (pcr) is an important tool for pathogen detection, but historically, it has not been possible to accurately identify pcr products without sequencing, southern blots, or dot-blots. microarrays can be coupled with pcr where they serve as a set of parallel dot-blots to enhance product detection and identification. microarrays are composed of many discretely located probes on a solid substrate such as glass. each probe is composed of a sequence that is complimentary to a pa ... | 2003 | 12654494 |
| the use of nasba for the detection of microbial pathogens in food and environmental samples. | the isothermal amplification method nucleic acid sequence-based amplification (nasba), which amplifies rna, has been reported as useful for the detection of microbial pathogens in food and environmental samples. methods have been published for campylobacter spp., listeria monocytogenes and salmonella enterica ser. enteritidis in various foods and for cryptosporidium parvum in water. both 16s rrna and various mrnas have been used as target molecules for detection; the latter may have advantages i ... | 2003 | 12654488 |
| high-level expression of the listeria monocytogenes listeriolysin o in escherichia coli and preliminary characterization of the purified protein. | listeriolysin o (llo) is a cholesterol-binding sulfhydryl-activated hemolysin encoded by listeria monocytogenes hlya gene. after analyzing the nucleotide coding sequence of this gene from the atcc 9525 l. monocytogenes strain, we cloned it in a pet vector for expression in escherichia coli. thanks to the optimization of the induction protocol, we achieved a high-level llo synthesis (about 10% of total cell proteins) in hemolytically active form. the expressed hemolysin was then purified to homog ... | 2003 | 12651110 |
| occurrence of listeria sp and l monocytogenes in sewage sludge used for land application: effect of dewatering, liming and storage in tank on survival of listeria species. | the application of sewage sludge to agricultural land is widely used in france. to determine the impact of sludge treatments, concentrations of listeria sp., listeria monocytogenes and faecal indicators were monitored in five types of sludge from three sewage treatment plants in angers (france) and its suburbs over a 1-year period. on the whole, bacteria were reduced in numbers through sludge treatments. apart from liming, which leads to reduced levels of bacteria below detection limits, other s ... | 2003 | 12648847 |
| efficiency of high pressure treatment for destruction of listeria monocytogenes in fruit juices. | the objective of this study was to compare high pressure resistance of listeria monocytogenes strains at 25 degrees c and 50 degrees c at 350 mpa and to use high pressure (250 mpa and 350 mpa) at 30 degrees c and 40 degrees c for the inactivation of the relatively most pressure resistant strain inoculated in pasteurized apple, apricot, cherry and orange juices. l. monocytogenes ca was found to be the relatively most pressure resistant strain and increasing pressurization from 250 mpa to 350 mpa ... | 2003 | 12648846 |
| quantitative risk assessment of listeria monocytogenes in ready-to-eat foods: the fao/who approach. | quantitative microbiological risk assessment is a very new and unique scientific approach able to link, for the first time, data from food (in the farm-to-fork continuum) and the various data on human disease to provide a clear estimation of the impact of contaminated food on human public health. the food and agriculture organization of the united nations (fao) and the world health organization (who) have recently launched risk assessment studies of a number of pathogen-food commodity combinatio ... | 2003 | 12648845 |
| production of il-12 and il-18 in human dendritic cells upon infection by listeria monocytogenes. | dendritic cells (dcs) are major antigen-presenting cells of the immune system, which need to be activated in order to initiate an immune response. here, we describe the immunostimulatory effects on human monocyte-derived dcs observed upon infection with listeria monocytogenes or after treatment with listerial lipoteichoic acid (lta) and lipopolysaccharide (lps), respectively. all stimuli caused upregulation of costimulatory molecules, induced t-cell proliferative responses and secretion of cytok ... | 2003 | 12648844 |
| tailoring host immune responses to listeria by manipulation of virulence genes -- the interface between innate and acquired immunity. | although attenuated strains of microbial pathogens have triggered vaccine development from its origin, the role of virulence factors in determining host immunity has remained largely unexplored. using the murine listeriosis model, we investigated whether the induction and expansion of protective and inflammatory t cell responses may be modified by selective manipulation of virulence genes. we intentionally deleted specific genes of listeria monocytogenes, including those encoding the positive re ... | 2003 | 12648843 |
| cd8 t cell immunome analysis of listeria monocytogenes. | the identification of t cell epitopes is crucial for the understanding of the host response during infections with pathogenic microorganisms. generally, the identification of relevant t cell responses is based on the analysis of t cell lines propagated in vitro. we used an ex vivo approach for the analysis of the cd8 t cell response against listeria monocytogenes that is based upon the fractionation of naturally processed antigenic peptides and subsequent analysis with t cells in an enzyme-linke ... | 2003 | 12648842 |
| characterization of listeria monocytogenes recovered from 41 cases of sporadic listeriosis in austria by serotyping and pulsed-field gel electrophoresis. | 41 clinical listeria monocytogenes strains recovered from seven feto-maternal and 34 non-pregnancy associated cases of human listeriosis documented between 1997 and 2000 underwent serotyping and typing by pulsed-field gel electrophoresis (pfge) applying the enzymes asci, apai and smai. the pulsotypes of the clinical strains were compared to the pulsotypes of three l. monocytogenes strains isolated from healthy fecal carriers and nine reference strains isolated from seven outbreaks in europe and ... | 2003 | 12648841 |
| nucleic acid-based, cultivation-independent detection of listeria spp and genotypes of l monocytogenes. | based on comparative analysis of 16s rrna gene sequences, two oligonucleotide probes for in situ detection of all members of the genus listeria were designed. these probes allowed fast and reliable in situ detection of listeria spp. even in complex samples like raw milk. almost full-length iap (invasion-associated protein) gene sequences were determined for 69 listeria monocytogenes strains of all 13 known serotypes. a comparison of these sequences revealed that the l. monocytogenes strains can ... | 2003 | 12648840 |
| comparison of the genome sequences of listeria monocytogenes and listeria innocua: clues for evolution and pathogenicity. | listeria monocytogenes, an invasive opportunistic, food-borne pathogen, remains one of the leading causes of mortality from food-borne infections. the recently determined complete genome sequences of l. monocytogenes strain egde and of that of the closely related non-pathogenic species listeria innocua strain clip11262 enhance our knowledge of the genetic basis of the virulence of l. monocytogenes and advance our understanding of the evolution of these listeria species. both genomes encode a hig ... | 2003 | 12648839 |
| listeriosis: therapeutic options. | since overt listeriosis occurs mainly in immunocompromised persons it is quite consistent to try to restore the hampered defence system by supportive measurements. for direct antimicrobial treatment a series of different antibiotics is available, since listeria strains isolated from patients are in general susceptible to a wide range of antibiotics, except fosfomycin, quinolones and cephalosporins of the third generation, although a few exceptional strains exist. unfortunately, most antibiotics ... | 2003 | 12648838 |
| history and epidemiology of listeriosis. | listeriae are used as a tool by different specialities in biomedical research. there are now at least four major fields of interest in listeria: (1). the role in medical microbiology: listeria monocytogenes causes severe diseases of men and animals and is difficult to treat; (2). the role in food microbiology: listeria is a food-borne pathogen and is found in various food items; (3). the role in cell biology: l. monocytogenes is a facultative intracellular parasite having an intense cross-talk a ... | 2003 | 12648837 |
| listeria monocytogenes: diagnostic problems. | the first isolation methods for the detection of listeria spp. were generally based on the direct culture of samples on simple agar media, but isolation of the pathogenic listeria monocytogenes was difficult. in time, new techniques were developed, based on a variety of selective and elective agents in isolation and enrichment media, which gained better and quicker results. current reference methods allow the recovery of l. monocytogenes from a variety of foods with relative ease. however, more ... | 2003 | 12648836 |
| listeria: growth, phenotypic differentiation and molecular microbiology. | the identification of listeria species is based on a limited number of biochemical markers, among which absence or presence of hemolysis and arylamidase are used to differentiate between l. monocytogenes and l. innocua. the camp (christie, atkins, munch-petersen) test must be interpreted with caution. chromogenic media are based on both the specific chromogenic detection of phosphatidylinositol phospholipase c and the xylose fermentation and give specific and direct identification of l. monocyto ... | 2003 | 12648835 |
| murine model of pregnancy-associated listeria monocytogenes infection. | listeria monocytogenes has been recognized as a significant pathogen, occurring worldwide, capable of causing animal and human infections. in its most severe form, listeriosis is an invasive disease that affects immunocompromised patients. additionally, pregnant women represent a high-risk group for l. monocytogenes infection. abortion, stillbirth or severe neonatal infection can be the serious outcome of such an infection. in an experimental murine model of pregnancy-associated listeriosis we s ... | 2003 | 12648834 |
| listeriosis: clinical presentation. | listeria monocytogenes is an uncommon cause of illness in the general population. however, this bacterium is an important cause of severe infections in neonates, pregnant women, the elderly, transplant recipients and other patients with impaired cell-mediated immunity. various clinical syndromes due to l. monocytogenes have been described such as sepsis, central nervous system infections, endocarditis, gastroenteritis and localized infections. a review of the clinical presentation of listeriosis ... | 2003 | 12648833 |
| bacteriocin-like substance (bls) production in aeromonas hydrophila water isolates. | 30 aeromonas hydrophila water isolates were tested for bacteriocin-like substance (bls) production using a target panel of closely related microorganisms and other gram-positive and gram-negative bacteria, including food-borne pathogens. a. hydrophila showed antibacterial activity against one or more indicator microorganisms, but the activity emerged only with non-phylogenetically related genera or species. in particular all a. hydrophila showed antibacterial activity against one or more of the ... | 2003 | 12644237 |
| multi locus fingerprinting of listeria monocytogenes by sequence-specific labeling of dna probes combined with array hybridization. | we have developed an alternative multi locus sequence typing (mlst) approach that targets the variable genetic changes directly in a dna array format. our approach is based on dna array hybridization in combination with sequence-specific labeling of oligonucleotide probes. listeria monocytogenes was chosen for the development and evaluation of the assay. the genes hlya, iap, flaa, inla and acta were targeted. twenty-nine suitable probe regions were identified within these genes. the dna array re ... | 2003 | 12644221 |
| an improved selective isolation medium for the recovery of listeria monocytogenes from smoked fish. | the aim of this study was to improve the selective isolation of listeria monocytogenes from smoked haddock fillets. | 2003 | 12641717 |
| measles virus infection results in suppression of both innate and adaptive immune responses to secondary bacterial infection. | among infectious agents, measles virus (mv) remains a scourge responsible for 1 million deaths per year and is a leading cause of childhood deaths in developing countries. although mv infection itself is not commonly lethal, mv-induced suppression of the immune system results in a greatly increased susceptibility to opportunistic bacterial infections that are largely responsible for the morbidity and mortality associated with this disease. despite its clinical importance, the underlying mechanis ... | 2003 | 12639986 |
| effects of gamma ray and electron-beam irradiations on survival of anaerobic and facultatively anaerobic bacteria. | an extension of the approval for food irradiation is desired due to the increase in the incidence of food poisoning in the world. one anaerobic (clostridium perfringens) and four facultatively anaerobic (bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, and salmonella enteritidis) bacteria irradiated with gamma ray or electron beam (e-beam) were tested in terms of survival on agar under packaging atmosphere. using pouch pack, effects of two irradiations on survival of anaerobic ... | 2002 | 12638185 |
| rapid detection of escherichia coli o157:h7 inoculated in ground beef, chicken carcass, and lettuce samples with an immunomagnetic chemiluminescence fiber-optic biosensor. | a biosensor was evaluated with regard to its usefulness in the rapid detection of escherichia coli o157:h7 inoculated in ground beef, chicken carcass, and romaine lettuce samples. the biosensor consisted of a chemiluminescence reaction cell, a fiber-optic light guide, and a luminometer linked to a personal computer in conjunction with immunomagnetic separation. the samples inoculated with e. coli o157:h7 were first centrifuged and suspended in buffered peptone water and then incubated with anti- ... | 2003 | 12636312 |
| evaluation and interlaboratory validation of a selective agar for phosphatidylinositol-specific phospholipase c activity using a chromogenic substrate to detect listeria monocytogenes from foods. | phosphatidylinositol-specific phospholipase c (pi-plc) activity is a potential virulence factor and is exhibited only by the listeria species listeria monocytogenes and listeria ivanovii. a chromogenic substrate for the direct detection of pi-plc activity is available in a new medium (bcm l. monocytogenes plating agar). the use of a chromogenic substrate offers a mechanism with which to directly screen for l. monocytogenes and l. ivanovii other than the esculin used in oxford (oxf) and palcam (p ... | 2003 | 12636298 |
| detection of listeria monocytogenes in salmon using the probelia polymerase chain reaction system. | a validation was conducted on the performance of a commercially available polymerase chain reaction (pcr) kit (probelia) in comparison with international organization for standardization (iso) method 11290-1 (adopted as an australian new zealand standard method, as/nzs 1766.2.16.1:1998) for the detection of listeria monocytogenes in salmon samples. the validation was conducted following the guidelines of an australian new zealand standard (guide to determining the equivalence of food microbiolog ... | 2003 | 12636297 |
| microbiological study of ready-to-eat salad vegetables from retail establishments uncovers a national outbreak of salmonellosis. | the increasing availability of bagged prepared salad vegetables reflects consumer demand for fresh, healthy, convenient, and additive-free foods that are safe and nutritious. during may and june 2001 a study of retail bagged prepared ready-to-eat salad vegetables was undertaken to determine the microbiological quality of these vegetables. examination of the salad vegetables revealed that the vast majority (3,826 of 3,852 samples; 99.3%) were of satisfactory or acceptable microbiological quality ... | 2003 | 12636292 |
| a novel method for the reduction of numbers of listeria monocytogenes cells by freezing in combination with an essential oil in bacteriological media. | the use of multiple freeze (-20 degrees c)-thaw cycles in combination with isoeugenol and polysorbate 80 was investigated as a method for the reduction of numbers of listeria monocytogenes cells in a bacteriological medium. three freeze (1 h, -20 degrees c)-thaw cycles in the presence of isoeugenol at concentrations of 0, 100, and 300 ppm resulted in average l. monocytogenes reductions of 0.69, 2.65, and 3.3 log10 mpn (most probable number) per ml, respectively. increasing the number of freeze-t ... | 2003 | 12636290 |
| viability of clostridium perfringens, escherichia coli, and listeria monocytogenes surviving mild heat or aqueous ozone treatment on beef followed by heat, alkali, or salt stress. | the threat of pathogen survival following ozone treatment of meat necessitates careful evaluation of the microorganisms surviving under such circumstances. the objective of this study was to determine whether sublethal aqueous ozone treatment (3 ppm of o3 for 5 min) of microorganisms on beef surfaces would result in increased or decreased survival with respect to subsequent heat, alkali, or nacl stress. a mild heat treatment (55 degrees c for 30 min) was used for comparison. reductions in three- ... | 2003 | 12636289 |
| impact of microbial ecology of meat and poultry products on predictions from exposure assessment scenarios for refrigerated storage. | a novel extension of traditional growth models for exposure assessment of food-borne microbial pathogens was developed to address the complex interactions of competing microbial populations in foods. scenarios were designed for baseline refrigeration and mild abuse of servings of chicken broiler and ground beef our approach employed high-quality data for microbiology of foods at production, refrigerated storage temperatures, and growth kinetics of microbial populations in culture media. simple p ... | 2003 | 12635734 |
| meconium enhances the growth of perinatal bacterial pathogens. | to demonstrate the effects of meconium on growth of bacterial pathogens, which are common causes of intra-amniotic infection and neonatal sepsis. | 2003 | 12634904 |
| capacity of ivanolysin o to replace listeriolysin o in phagosomal escape and in vivo survival of listeria monocytogenes. | listeriolysin o (llo, hly-encoded) is a major virulence factor secreted by the pathogen listeria monocytogenes. the amino acid sequence of llo shows a high degree of similarity with that of ivanolysin o (ilo), the cytolysin secreted by the ruminant pathogen listeria ivanovii. here, it was tested whether ilo could functionally replace llo by expressing the gene encoding ilo under the control of the hly promoter, in an hly-deleted strain of l. monocytogenes. it is shown that ilo allows efficient p ... | 2003 | 12634330 |
| can cold cuts kill? | 2003 | 12632673 | |
| subtyping listeria monocytogenes through the combined analyses of genotype and expression of the hlya virulence determinant. | a major challenge for listeria monocytogenes diagnostics is that this bacterium is ubiquitous in the environment, and that only a small fraction of the lineages are potential human pathogens. the aim of this work was to obtain a better subtyping of l. monocytogenes through utilization of combined analyses of genotype and the expression of the virulence determinant hlya. | 2003 | 12631208 |
| molecular epidemiological survey of listeria monocytogenes in broilers and poultry products. | to investigate the prevalence of listeria monocytogenes in poultry products, and to elucidate whether poultry products may be linked to listeriosis cases. a further goal was to identify contamination routes for l. monocytogenes to broiler carcasses. | 2003 | 12631199 |
| autolytic activity and pediocin-induced lysis in pediococcus acidilactici and pediococcus pentosaceus strains. | to evaluate the autolytic phenotype of pediococcus acidilactici and p. pentosaceus, the peptidoglycan hydrolases content and the effect of pediocin ach/pa-1 and autolysins on cell lysis. | 2003 | 12631191 |
| engineered recombinant peanut protein and heat-killed listeria monocytogenes coadministration protects against peanut-induced anaphylaxis in a murine model. | peanut allergy (pna) is the major cause of fatal and near-fatal anaphylactic reactions to foods. traditional immunotherapy using peanut (pn) protein is not an option for pna therapy because of the high incidence of adverse reactions. we investigated the effects of s.c. injections of engineered (modified) recombinant pn proteins and heat-killed listeria monocytogenes (hklm) as an adjuvant on anaphylactic reactions in a mouse model of pn allergy. pn-allergic c3h/hej mice were treated s.c. with a m ... | 2003 | 12626588 |
| use of listeriolysin o and internalin a in a seroepidemiological study of listeriosis in swiss dairy cows. | recombinant listeriolysin o and internalin a were used as antigens in an enzyme-linked immunosorbent assay (elisa) for the specific detection of anti-listeria monocytogenes antibodies in cattle. the results showed sensitivities and specificities of 82 and 92%, respectively, for the listeriolysin o elisa, and 100 and 90%, respectively, for the internalin a elisa, respectively. the test may be useful for the confirmation of listeria-related abortions and mastitis but does not seem to be indicated ... | 2003 | 12624029 |
| transcriptome analysis of listeria monocytogenes identifies three groups of genes differently regulated by prfa. | prfa is the major regulator of listeria virulence gene expression. this protein is a member of the crp/fnr family of transcription regulators. to gain a deeper understanding of the prfa regulon, we constructed a whole-genome array based on the complete genome sequence of listeria monocytogenes strain egde and evaluated the expression profiles of the wild-type egde and a prfa-deleted mutant (egde delta prfa). both strains were grown at 37 degrees c in brain-heart infusion broth (bhi) and bhi supp ... | 2003 | 12622816 |
| a pediocin-producing lactobacillus plantarum strain inhibits listeria monocytogenes in a multispecies cheese surface microbial ripening consortium. | the growth of listeria monocytogenes wslc 1364, originating from a cheese-borne outbreak, was examined in the presence and in the absence of a pediocin ach-producing lactobacillus plantarum strain on red smear cheese. nearly complete inhibition was observed at 10(2) cfu of l. monocytogenes per ml of salt brine solution, while contamination with listeria mutants resistant to pediocin resulted in high cell counts of the pathogen on the cheese surface. the inhibition was due to pediocin ach added t ... | 2003 | 12620882 |
| kdpe and a putative rsbq homologue contribute to growth of listeria monocytogenes at high osmolarity and low temperature. | the kdp locus of listeria monocytogenes encodes products with homology to structural proteins of a high-affinity potassium uptake system and to a two-component signal transduction system commonly involved in controlling gene expression. we have investigated the role of kdpe, encoding the transcriptional response regulator, as well as of the downstream gene, orfx, in adaptation of l. monocytogenes egd to nacl and low temperature. when grown in chemically defined medium the addition of nacl to 2% ... | 2003 | 12620626 |
| effect of lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on the susceptibility to listeria infection. | we studied the effect of lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) on the susceptibility to listeria infection of offspring in c57bl /6ncji mice. the offspring were nursed by tcdd-treated dams and exposed to tcdd from birth to weaning via milk. the exposure had little effect on the weights of immune organs and the spleen or the thymus cell population in the dams and offspring, but it enhanced the production of tumor necrosis factor alpha (tnf-alpha) and interferon gamma ... | 2003 | 12619678 |
| flt3 ligand-treated neonatal mice have increased innate immunity against intracellular pathogens and efficiently control virus infections. | flt-3 ligand (fl), a hematopoetic growth factor, increases the number of dendritic cells (dcs), b cells, and natural killer cells in adult mice but the effect in neonates was unknown. we show that fl treatment of newborn mice induced a >100-fold increase in the innate resistance against infection with herpes simplex virus type 1 and listeria monocytogenes. this resistance required interferon (ifn)-alpha/beta for viral and interleukin (il)-12 for bacterial infections. long-term survival after vir ... | 2003 | 12615899 |
| il-13 effector functions. | il-13 was first recognized for its effects on b cells and monocytes, where it upregulated class ii expression, promoted ige class switching and inhibited inflammatory cytokine production. it was also thought to be functionally redundant with il-4. however, studies conducted with knockout mice, neutralizing antibodies, and novel antagonists demonstrate that il-13 possesses several unique effector functions that distinguish it from il-4. resistance to most gastrointestinal nematodes is mediated by ... | 2003 | 12615888 |
| febrile gastroenteritis after eating on-farm manufactured fresh cheese--an outbreak of listeriosis? | an outbreak of febrile gastroenteritis affected consumers of on-farm manufactured dairy products from a summer farm in sweden. symptoms included diarrhoea, fever, stomach cramps and vomiting in 88, 60, 54 and 21% of cases identified. the median incubation period was 31 h. a cohort study with 33 consumers showed an attack rate of 52% and an association between the total amount of product eaten and illness (p=0.07). twenty-seven of 32 (84%) stool samples cultured for listeria monocytogenes tested ... | 2003 | 12613748 |
| antimicrobial efficacy of a silver-zeolite matrix coating on stainless steel. | a silver- and zinc-containing zeolite matrix (agion) used as a coating for stainless steel was tested for antimicrobial efficacy against escherichia coli 25922, staphylococcus aureus 25923, pseudomonas aeruginosa 27853, and listeria monocytogenes 7644. assays were performed on flat coupon surfaces and in formed steel cups. agion reduced microbial colony-forming units when compared to uncoated steel surfaces under all conditions tested. percent reductions ranged from 84.536 to 99.999 after 4 h ex ... | 2003 | 12612784 |
| [an elderly case with listeria monocytogenes sepsis and pulmonary non-tuberculous mycobacterial infection]. | a 88-year-old woman, who had lived in a nursing home, was admitted to our hospital because of the suspicion of pulmonary tuberculosis. she had a cough, fever and diarrhea on admission. she suffered from sepsis because listeria monocytogenes was isolated from only the blood culture twice. we immediately administered imipenem/cilastatin to her on admission. she simultaneously had pulmonary non-tuberculous mycobacterial infection because the chest roentgenogram showed a cavity in the right upper lu ... | 2002 | 12607349 |
| [studies on the effect of combined treatment of irradiation with vacuum packaging on ready-to-eat meat products contaminated with listeria monocytogenes and spoilage bacteria]. | altogether one hundred and fifty samples of beijing roast duck, roast chicken and cooked meat products inoculated with approximately 1.5 x 10(3)-2.3 x 10(3) cfu/g l. monocytogenes are packed under vacuum and are irradiated with 60co radiation at doses of 1.0, 2.0, 2.5 and 3 kgy. the results showed that l. monocytogenes in samples can be eliminated by a dose of 2.5 kgy. the resistance of the model strain 54004 to irradiation is stranger than three isolates (x20, g4 and g2). in addition, the spoil ... | 2002 | 12600048 |
| [isolation and identification of listeria monocytogenes and listeria spp. in dry sausages obtained in markets in the city of la plata, argentina]. | a total of 60 samples of dry sausages were analyzed (50 of "salami" and 10 of "chorizo" "candelario" type) obtained at random in markets authorized for their commercialization, for the purpose of evidencing the presence of bacteria of the genus listeria (listeria monocytogenes and listeria spp.). the results obtained in salami were the following: 10 (20%) isolates of listeria spp., were characterized: 1 (2%) strain as l. monocytogenes type 1, 7 (14%) strains as l. innocua, 2 (4%) strains as l. w ... | 2002 | 12600006 |
| in vitro evaluation of the cytotoxicity of two root canal sealers on macrophage activity. | although some studies have been concerned with the cytotoxicity of endodontic sealers and their components, few have approached the effects of endodontic sealers on macrophage viability and activity. in this study the effect of two zinc oxide-eugenol-based sealers, freshly prepared or after setting for 24 h, was determined on macrophage activity in vitro. sealers were placed inside a glass capillary tube and added to mouse-elicited macrophage cultures. sealers did not affect macrophage viability ... | 2003 | 12597705 |
| prevalence of listeria spp. in feces and carcasses at a lamb packing plant in brazil. | the objective of this work was to study the occurrence of listeria species in feces and on dressed and cooled carcasses of lambs at a packing plant in brazil. listeria spp. were recovered on oxford and palcam agars. the 35 fecal samples yielded listeria welshimeri (20%) and listeria innocua (8.6%). the 69 carcass samples yielded l. innocua (34.8%), listeria monocytogenes (4.3%), and listeria ivanovii (1.5%). more listeria spp. were recovered with two selective agars than with either agar alone. | 2003 | 12597497 |
| bacteriology and storage life of moisture-enhanced pork. | this study was undertaken to determine the impact of the moisture enhancement process on the bacterial contamination and storage life of vacuum-packaged pork loins. bone-in and boneless pork loins injected with brine (sodium chloride, sodium phosphate, lemon juice) were obtained from a commercial processing facility and stored for 5 weeks in vacuum packaging at 2 and 5 degrees c. at weekly intervals, samples were excised to determine numbers of spoilage bacteria and pathogens. the loins were sub ... | 2003 | 12597491 |
| impact of preheating on the behavior of listeria monocytogenes in a broth that mimics camembert cheese composition. | the effect of preheating on the survival of l. monocytogenes in richard's broth, which mimics the composition of camembert cheese composition, was examined. experiments were carried out to reproduce contamination of cheese with environmental heat-stressed cells of l. monocytogenes surviving hot-cleaning procedures. cells in mid-log phase were heated for 30 min at 56 degrees c before being inoculated into richard's broth. the phs and temperatures of richard's broth were chosen to recreate the con ... | 2003 | 12597487 |
| assessment of control measures to achieve a food safety objective of less than 100 cfu of listeria monocytogenes per gram at the point of consumption for fresh precut iceberg lettuce. | the important new concept of the food safety objective (fso) offers a strategy to translate public health risk into a definable goal such as a specified maximum frequency or concentration of a hazardous agent in a food at the time of consumption that is deemed to provide an appropriate level of health protection. for the foodborne pathogen listeria monocytogenes, there is a proposed fso of < 100 cfu/g in ready-to-eat (rte) products at the time of consumption. fresh precut iceberg lettuce is one ... | 2003 | 12597486 |
| typing of listeria monocytogenes isolates originating from the food processing industry with automated ribotyping and pulsed-field gel electrophoresis. | a total of 486 listeria monocytogenes isolates originating from 17 finnish food processing plants (representing meat, poultry, fish, and dairy production) were collected and typed by automated ribotyping using ecori as the restriction enzyme. the isolates were divided into 16 different ribotypes (rts). some of these isolates (121), representing all ecori types and 16 food plants, were subjected to ribotyping with the pvuii enzyme, to pulsed-field gel electrophoresis (pfge) typing with asci and s ... | 2003 | 12597485 |
| polymerase chain reaction detection of listeria monocytogenes on frankfurters using oligonucleotide primers targeting the genes encoding internalin ab. | a polymerase chain reaction (pcr) assay targeting the genes encoding internalin ab (inlab) was developed for detecting listeria monocytogenes in pure cell cultures and on artificially contaminated frankfurters. four sets of oligonucleotide primers were evaluated. the set targeting a 902-bp region of the inlab gene was the most specific. this pcr product was detected in 51 l. monocytogenes strains belonging to four different serogroups (1/2a, 1/2b, 1/2c, and 4b). in contrast, the pcr product was ... | 2003 | 12597483 |
| survey of retail alfalfa sprouts and mushrooms for the presence of escherichia coil o157:h7, salmonella, and listeria with bax, and evaluation of this polymerase chain reaction-based system with experimentally contaminated samples. | bax, a polymerase chain reaction (pcr)-based pathogen detection system, was used to survey retail sprouts and mushrooms for contamination with escherichia coli o157:h7, salmonella, listeria spp., and listeria monocytogenes. no salmonella or e. coli o157:h7 was detected in the 202 mushroom and 206 alfalfa sprout samples screened. l. monocytogenes was detected in one sprout sample, and seven additional sprout samples tested positive for the genus listeria. bax also detected listeria species in 17 ... | 2003 | 12597474 |
| n-terminal e-cadherin peptides act as decoy receptors for listeria monocytogenes. | the observation that e-cadherin is the principal epithelial receptor for the bacterial pathogen listeria monocytogenes led us to investigate whether n-terminal fragments of e-cadherin containing the l. monocytogenes binding domain could inhibit entry of the bacteria into cultured epithelial cells. here we demonstrate that a conditioned medium from a gastric cancer cell line (kato iii) that carries a truncating cdh-1 mutation 3' of the l. monocytogenes binding domain can inhibit the uptake of the ... | 2003 | 12595481 |
| nonhuman primate model for listeria monocytogenes-induced stillbirths. | listeria monocytogenes, isolated from outbreaks in either human or nonhuman primate populations, was administered orally at doses ranging from 10(6) to 10(10) cfu. four of 10 treated animals delivered stillborn infants. l. monocytogenes was isolated from fetal tissue, and the pathology was consistent with l. monocytogenes infection as the cause of pregnancy loss. for all pregnancies resulting in stillbirths, l. monocytogenes was isolated from maternal feces, indicating that l. monocytogenes had ... | 2003 | 12595480 |
| effects of ectopically expressed neuronal wiskott-aldrich syndrome protein domains on rickettsia rickettsii actin-based motility. | neuronal wiskott-aldrich syndrome protein (n-wasp) and the actin-related protein 2/3 (arp2/3) complex have emerged as critical host proteins that regulate pathogen actin-based motility. actin tail formation and motility in listeria monocytogenes require the arp2/3 complex but bypasses n-wasp signaling. motility of shigella flexneri and vaccinia virus requires both n-wasp and the arp2/3 complex. functional roles for these cytoskeletal regulatory proteins in actin-based motility of rickettsia rick ... | 2003 | 12595475 |
| expression of truncated internalin a is involved in impaired internalization of some listeria monocytogenes isolates carried asymptomatically by humans. | fourteen human carriage listeria monocytogenes isolates were compared to sporadic and epidemic-associated human strains in order to ascertain the pathogenic behavior of these unrecognized asymptomatic strains. experimental infection of 14-day-old chick embryos revealed that the majority of the carriage strains were attenuated for virulence. of the 10 attenuated carriage strains, 5 were affected in their invasion capacities in vitro. western blot analysis with antibody directed against inla, the ... | 2003 | 12595435 |
| enteric salmonella infection inhibits paneth cell antimicrobial peptide expression. | paneth cells, highly secretory epithelial cells found at the bases of small intestinal crypts, release a variety of microbicidal molecules, including alpha-defensins and lysozyme. the secretion of antimicrobials by paneth cells is thought to be important in mucosal host defense against invasion by enteric pathogens. we explored whether enteric pathogens can interfere with this arm of defense. we found that oral inoculation of mice with wild-type salmonella enterica serovar typhimurium decreases ... | 2003 | 12595421 |
| enteral immunization with attenuated recombinant listeria monocytogenes as a live vaccine vector: organ-dependent dynamics of cd4 t lymphocytes reactive to a leishmania major tracer epitope. | listeria monocytogenes is considered as a potential live bacterial vector, particularly for the induction of cd8 t cells. the cd4 t-cell immune response triggered after enteral immunization of mice has not yet been thoroughly characterized. the dynamics of gamma interferon (ifn-gamma)- and interleukin-4 (il-4)-secreting cd4 t cells were analyzed after priming through intragastric delivery of an attenuated delta acta recombinant l. monocytogenes strain expressing the leishmania major lack protein ... | 2003 | 12595418 |
| severe impairment in early host defense against listeria monocytogenes in mice deficient in acid sphingomyelinase. | the phagolysosomal compartment is crucial for the defense against infection with intracellular pathogens. within this compartment, the tnf- and ifn-gamma-responsive acid sphingomyelinase (asmase) generates the signaling molecule ceramide, resulting in the activation of proteases like cathepsin d. to investigate the possible role of asmase as a mediator of the antibacterial effects of tnf and ifn-gamma, asmase(-/-) mice were infected with listeria monocytogenes. asmase(-/-) mice showed a dramatic ... | 2003 | 12594290 |
| adaptive and cross-adaptive responses of persistent and non-persistent listeria monocytogenes strains to disinfectants. | persistent and non-persistent listeria monocytogenes strains were tested for initial resistance and adaptive and cross-adaptive responses towards two quaternary ammonium compounds, alkyl-benzyl-dimethyl ammonium chloride and n-alkyldimethyl ethylbenzyl ammonium chloride, one tertiary alkylamine, 1,3-propanediamine-n-(3-aminopropyl)n-dodecyl, sodium hypochlorite and potassium persulphate. the initial resistance of two persistent and two non-persistent l. monocytogenes strains was observed to diff ... | 2003 | 12593929 |
| microbial quality of shrimp products of export trade produced from aquacultured shrimp. | bacteriological quality of individually quick frozen (iqf) shrimp products produced from aquacultured tiger shrimp (penaeus monodon) has been analysed in terms of aerobic plate count (apc), coliforms, escherichia coli, coagulase-positive staphylococci, salmonella, and listeria monocytogenes. eight hundred forty-six samples of raw, peeled, and deveined tail-on (rpto), 928 samples of cooked, peeled, and deveined tail-on (cpto), 295 samples of headless, undeveined shell-on (hlso), and 141 samples o ... | 2003 | 12593924 |
| the force-velocity relationship for the actin-based motility of listeria monocytogenes. | the intracellular movement of the bacterial pathogen listeria monocytogenes has helped identify key molecular constituents of actin-based motility (recent reviews ). however, biophysical as well as biochemical data are required to understand how these molecules generate the forces that extrude eukaryotic membranes. for molecular motors and for muscle, force-velocity curves have provided key biophysical data to distinguish between mechanistic theories. here we manipulate and measure the viscoelas ... | 2003 | 12593799 |
| [listeria rhombencephalitis. neuroradiological findings]. | rhombencephalitis due to listeria monocytogenes is an uncommon and serious form of brainstem infection. the disease has a characteristic biphasic course: a nonspecific prodrome of headache, nausea or vomiting, and fever lasting for a several days is followed by progressive asymmetrical cranial-nerve palsies. we report two cases of listeria monocytogenes rhombencephalitis. the first case is a previously healthy 20 year-old-man who developed fever, headache, nausea and vomiting, followed by numbne ... | 2003 | 12590380 |
| identification and characterisation of regions in the cellular protein laxp180 and the listeria monocytogenes surface protein acta necessary for the interaction of the two proteins. | the listeria monocytogenes surface protein acta is an important virulence factor that plays an essential role in intracellular movement of listeria cells by inducing actin polymerisation. the acta protein is known to interact with several mammalian proteins including the phosphoprotein vasp, actin and the arp2/3 complex. in a search for additional acta-binding proteins we recently employed the yeast two-hybrid system to search for proteins that interact with acta, and identified, among others, t ... | 2003 | 12589435 |
| partial characterization of bacteriocins produced by environmental strain enterococcus faecium ek13. | the partial characterization of bacteriocins produced by an environmental strain enterococcus faecium ek13, isolated from cattle dung water. | 2003 | 12588562 |
| listeriosis in a free-ranging colobus monkey (colobus guereza caudatus) in kenya. | 2003 | 12585602 | |
| different cellular fatty acid pattern behaviours of two strains of listeria monocytogenes scott a and cnl 895807 under different temperature and salinity conditions. | cells of two strains of listeria monocytogenes cnl 895807 and scott a were grown to late exponential phase at different growth temperatures (37, 20 and 4 degrees c) with or without nacl (7%), and their fatty acid compositions were analysed. the results showed that low thermal adaptation response of l. monocytogenes cnl was different than that of the scott a strain, and it was based on both an increase of anteiso-branched-chain fatty acids and a significant decrease of straight-chain fatty acids. ... | 2003 | 12583912 |
| identification and disruption of btla, a locus involved in bile tolerance and general stress resistance in listeria monocytogenes. | a transposon tn917 mutant of listeria monocytogenes l028 was isolated on the basis of reduced growth on agar adjusted to ph 5.5. the disrupted gene, designated btla (bile tolerance locus), encodes a putative secondary transporter of the major facilitator superfamily, which has significant homology to yxio in bacillus subtilis (lmo1417 in l. monocytogenes egde). the mutant demonstrated decreased growth rates relative to the wild-type when grown in sub-lethal levels of various stressors (acid, sal ... | 2003 | 12583894 |
| incidence of fecal carriage of listeria monocytogenes in three healthy volunteers: a one-year prospective stool survey. | the aim of this study was to establish the incidence of fecal carriage of listeria monocytogenes in healthy adults. a total of 868 stool specimens from three healthy volunteers (1 male and 2 females; ages 44, 39, and 60 years) were collected between 1 october 2000 and 30 september 2001. culture was performed using fraser broth and palcam selective agar plates. polymerase chain reaction (pcr) was performed using probelia listeria monocytogenes (biorad, france). overall, listeria monocytogenes was ... | 2003 | 12582739 |
| in vitro study of listeria monocytogenes infection to murine primary and human transformed b cells. | immunity to listeria monocytogenes is largely mediated by t lymphocytes. recently, b lymphocytes or their secreted products are implicated to provide immunity against l. monocytogenes infection. to understand whether l. monocytogenes can infect and kill b cells as a possible strategy to initiate an infection, we examined the effects of l. monocytogenes on a human b lymphoma (ramos ra-1) and mouse primary b cells in vitro. l. monocytogenes infection resulted in significantly (p<or=0.05) high cyto ... | 2003 | 12581746 |
| the effect of electrolyzed oxidative water applied using electrostatic spraying on pathogenic and indicator bacteria on the surface of eggs. | research was conducted to compare the effectiveness of electrolyzed oxidative (eo) water applied using an electrostatic spraying system (ess) for killing populations of bacteria that are of concern to the poultry industry. populations of pathogenic bacteria (salmonella typhimurium, staphylococcus aureus, and listeria monocytogenes), and the indicator bacterium escherichia coli were applied to eggs and allowed to attach for 1 h. eo water completely eliminated all salmonella typhimurium on 3, 7, 1 ... | 2003 | 12580260 |
| inhibitory activity of spices and essential oils on psychrotrophic bacteria. | this study was designed to evaluate "in vitro" the inhibitory effects of spices and essential oils on the growth of psycrotrophic food-borne bacteria: aeromonas hydrophila, listeria monocytogenes and yersinia enterocolitica. the sensitivity to nine spices and their oils (chilli, cinnamon, cloves, ginger, nutmeg, oregano, rosemary, sage, thyme) was studied. antibacterial activity was evaluated on liquid and solid medium. spices: 1% concentration of each spice was added separately to triptic soy b ... | 2003 | 12578319 |
| inhibition of listeria monocytogenes lmg10470 by plantaricin ug1 in vitro and in beef meat. | the inhibition of listeria monocytogenes lmg 10470 occurred due to plantaricin ug1, but not to lactic acid produced by lactobacillus plantarum ug1 or its negatie variant (bac). partially purified plantaricin ug1 had a higher antilisterial activity in vitro and in meat than lactobacillus plantarum ug1 culture. plantaricin ug1 activity was higher in brain heart infusion (bhi) broth than in minced meat. the inhibitory effect of plantaricin ug1 against l. monocytogenes lmg10470 was dependent on its ... | 2002 | 12577590 |
| effect of reuterin produced by lactobacillus reuteri on the surface of sausages to inhibit the growth of listeria monocytogenes and salmonella spp. | reuterin is a bacteriocin produced by some strains of lactobacillus reuteri. the strain used in this study was isolated from raw milk from a dairy farm nearby ankara. beef sausage is a long years produced bratwurst style meat product in turkey, as well as in some other countries in the mediterranean region. sausages are produced by raw meat; sometimes lactic starter cultures are added or spontaneous fermentation is employed. the production and storage conditions of the product promotes the growt ... | 2002 | 12577589 |
| negative regulation of prfa, the key activator of listeria monocytogenes virulence gene expression, is dispensable for bacterial pathogenesis. | listeria monocytogenes is a facultative intracellular bacterial pathogen that regulates the expression of virulence-associated gene products in response to specific host cell compartment environments. the prfa protein of l. monocytogenes functions as a key regulatory factor required for the differential expression of bacterial virulence gene products within infected host cells. prfa both positively and negatively regulates its own expression, and while prfa positive regulation is required for ce ... | 2003 | 12576585 |
| role of cd4 t cell help and costimulation in cd8 t cell responses during listeria monocytogenes infection. | cd4 t cells are known to assist the cd8 t cell response by activating apc via cd40-cd40 ligand (l) interactions. however, recent data have shown that bacterial products can directly activate apc through toll-like receptors, resulting in up-regulation of costimulatory molecules necessary for the efficient priming of naive t cells. it remains unclear what role cd4 t cell help and various costimulation pathways play in the development of cd8 t cell responses during bacterial infection. in this stud ... | 2003 | 12574376 |
| h2-m3-restricted memory t cells: persistence and activation without expansion. | h2-m3-restricted t cells respond more rapidly to primary listeria monocytogenes infection than conventional mhc class ia-restricted t cells. reinfection with l. monocytogenes, while inducing explosive proliferation of h2-k(d)-restricted t cells, does not stimulate significant expansion of h2-m3-restricted ctl. these disparate responses to reinfection are apparent within 5 days of primary l. monocytogenes infection. however, h2-m3-restricted memory t cells are generated, and are indistinguishable ... | 2003 | 12574352 |
| preferential survival of cd8 t and nk cells expressing high levels of cd94. | the qa-1(b)/qdm tetramer binds to cd94/nkg2 receptors expressed at high levels on approximately 50% of murine nk cells. although very few cd8 t cells from naive mice express cd94/nkg2 receptors, approximately 50% of cd8 t cells taken from mice undergoing a secondary response against listeria monocytogenes (lm) are cd94(high) and bind the tetramer. although cd94(int) nk cells do not bind the tetramer, cd94(int) cd8 t cells do, and this binding is dependent on the cd8 coreceptor. we found that the ... | 2003 | 12574337 |
| development of a multilocus sequence typing method for analysis of listeria monocytogenes clones. | this study is a first step in the development of multilocus sequence typing (mlst) method for listeria monocytogenes. nine housekeeping genes were analyzed in a set of 62 strains isolated from different sources and geographic locations in spain. these strains were previously characterized by pulsed-field gel electrophoresis (pfge). because of low diversity, two loci were discarded from the study. the sequence analysis of the seven remaining genes showed 29 different allelic combinations, with 22 ... | 2003 | 12574278 |
| bartonella strains from ground squirrels are identical to bartonella washoensis isolated from a human patient. | the most likely animal source of a human case of cardiac disease in washoe county, nev., was identified by comparison of dna sequences of three genes (citrate synthase glta, 60-kda heat shock protein gene groel, and 16s rrna gene) of bartonella washoensis cultured from the human patient in question and of bartonella isolates obtained from the following nevada rodents: peromyscus maniculatus (17 isolates), tamias minimus (11 isolates), spermophilus lateralis (3 isolates), and spermophilus beechey ... | 2003 | 12574261 |
| mixed-genome microarrays reveal multiple serotype and lineage-specific differences among strains of listeria monocytogenes. | epidemiological studies and analysis of putative virulence genes have shown that listeria monocytogenes has diverged into several phylogenetic divisions. we hypothesize that similar divergence has occurred for many genes that influence niche-specific fitness and virulence and that identifying these differences may offer new opportunities for the detection, treatment, and control of this important pathogen. to explore this issue further, we developed a microarray composed of fragmented dna taken ... | 2003 | 12574259 |
| serotyping of listeria monocytogenes by enzyme-linked immunosorbent assay and identification of mixed-serotype cultures by colony immunoblotting. | routine analysis of listeria monocytogenes by serotyping using traditional agglutination methods is limited in use because of the expense and limited availability of commercially prepared antisera and intra- and interlaboratory discrepancies arising from differences in antiserum preparation and visual determination of agglutination. we have adapted a commercially available set of l. monocytogenes antisera to an enzyme-linked immunosorbent assay (elisa) format for high-throughput, low-cost seroty ... | 2003 | 12574247 |
| listeria monocytogenes infection as a complication of treatment with tumor necrosis factor alpha-neutralizing agents. | tumor necrosis factor alpha (tnfalpha) has been implicated in the pathogenesis of certain inflammatory diseases. two tnfalpha-neutralizing agents are licensed in the us. infliximab is licensed for the treatment of crohn's disease (cd) and, when used with methotrexate, for the treatment of rheumatoid arthritis (ra). etanercept is licensed for the treatment of ra, including juvenile ra, and, more recently, was licensed for the treatment of psoriatic arthritis. because of the potential for decrease ... | 2003 | 12571839 |
| detection of listeria monocytogenes from a model food by fluorescence resonance energy transfer-based pcr with an asymmetric fluorogenic probe set. | it has been shown that fluorescence resonance energy transfer (fret)-based pcr, including the taqman assay and molecular beacons, has potential for rapid detection of pathogens. in these promising real-time detection assays a single internal oligonucleotide probe labeled on both the 5' (reporter) and 3' (quencher) ends is used for selective generation of fluorescence. in this paper, we describe the use of a previously reported novel probe design for fret-based pcr detection of listeria monocytog ... | 2003 | 12571032 |
| three transporters mediate uptake of glycine betaine and carnitine by listeria monocytogenes in response to hyperosmotic stress. | the uptake and accumulation of the potent osmolytes glycine betaine and carnitine enable the food-borne pathogen listeria monocytogenes to proliferate in environments of elevated osmotic stress, often rendering salt-based food preservation inadequate. to date, three osmolyte transport systems are known to operate in l. monocytogenes: glycine betaine porter i (betl), glycine betaine porter ii (gbu), and a carnitine transporter opuc. we investigated the specificity of each transporter towards each ... | 2003 | 12571024 |
| growths kinetics comparison of clinical and seafood listeria monocytogenes isolates in acid and osmotic environment. | comparison of pathogenic bacterial strains of clinical origin with strains of the same species isolated from the environment may be a valuable tool for microbial risk assessment, especially for foodborne pathogens. thus, a number of listeria monocytogenes strains responsible for human cases of listeriosis, in relation to the consumption of contaminated seafood, have been compared with "natural" l. monocytogenes strains isolated from similar seafood products. complete factorial designs were used ... | 2003 | 12568752 |
| bad bugs: good for cancer therapy? | components of listeria and escherichia coli have been used to prime the immune system in a novel approach to fighting cancer. | 2003 | 12565001 |
| lineage relationship and protective immunity of memory cd8 t cell subsets. | memory cd8 t cells can be divided into two subsets, central (t(cm)) and effector (t(em)), but their lineage relationships and their ability to persist and confer protective immunity are not well understood. our results show that t(cm) have a greater capacity than t(em) to persist in vivo and are more efficient in mediating protective immunity because of their increased proliferative potential. we also demonstrate that, following antigen clearance, t(em) convert to t(cm) and that the duration of ... | 2003 | 12563257 |
| involvement of listeria monocytogenes in the abortive disease. | listeria monocytogenes, an intracellular facultative germ that causes the invasion, sometimes fatal, in susceptible hosts is a food borne pathogen with ubiquitary spread that has generated a public health problem for such risk groups as: pregnant women, foetuses, new borns. 504 women with abortive disease were serologically investigated in 1999 for serotype 1a circulating in romania. the most affected age group proved to be that in the range of 20-30 yrs: 378 (75%) cases. 107 (21.23%) female pat ... | 2001 | 12561675 |
| antibacterial activity of peptides derived from envelope glycoproteins of hiv-1. | recent reports have highlighted the anti-hiv-1 activities of defensins, whose structure and charge resemble portions of the hiv-1 transmembrane envelope glycoprotein gp41. the current report explores the obverse, whether peptides derived from hiv-1 envelope glycoproteins can exert antimicrobial activity. fifteen-residue peptides spanning the entire sequence of hiv-1(mn) gp120 and gp41 were subjected to radial diffusion assays against laboratory strains of escherichia coli and listeria monocytoge ... | 2003 | 12560103 |
| enhancing the immunogenicity of bioengineered listeria monocytogenes by passaging through live animal hosts. | bioengineered listeria monocytogenes can be used as a recombinant bacterial vaccine vector for the induction of strong cell-mediated immunity to passenger antigens. listeria loses virulence after undergoing bioengineering techniques, thus decreasing its efficacy as a vaccine vector. we addressed this problem by examining the virulence, and the ability to induce cd8(+) t-cells, of listeria monocytogenes vaccine strains before and after passaging through mice. we found that two in vivo passages ar ... | 2003 | 12559797 |
| discrimination among listeria monocytogenes isolates using a mixed genome dna microarray. | listeria monocytogenes can cause serious illness in humans, usually following the ingestion of contaminated food. epidemiologic investigation requires identification of specific isolates, usually done by a combination of serotyping and subtyping using pulsed-field gel electrophoresis (pfge). dna microarrays provide a new format to resolve genetic differences among isolates and, unlike pfge, to identify specific genes associated with the infecting pathogen. a 585 probe, mixed genome microarray wa ... | 2003 | 12554104 |
| [inflammatory bowel disease--do microorganisms play a role?]. | this review focuses on the potential pathogenic role of microorganisms in relation to inflammatory bowel diseases, i.e. crohn's disease and ulcerative colitis. pathogenic microorganism such as mycobacterium paratuberculosis, measles and mumps viruses, epstein-barr virus, and listeria monocytogenes are discussed, as well as involvement of the normal intestinal flora. furthermore, the influence of microorganisms in experimental animal colitis models is discussed. the available results are inconclu ... | 2002 | 12553118 |