Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| a spin-label study of biological membranes with special emphasis on calcium-induced lateral phase separation. | 1976 | 176878 | |
| an epidemic of diarrhoea in human neonates involving a reovirus-like agent and 'enteropathogenic' serotypes of escherichia coli. | during december 1974, an epidemic of diarrhoea occurred in the royal children's hospital, melbourne, in a ward caring for neonates with acute or chronic medical and surgical problems. electron microscopy of diarrhoeal faeces revealed a reovirus-like particle ('duovirus' or 'rotavirus') known to cause acute enteritis in older children. this virus is considered to have been primarily involved in the aetiology of the epidemic. in addition, three 'enteropathogenic' serotypes of escherichia coli were ... | 1976 | 175099 |
| human papillomavirus dna: physical map. | human papillomavirus (hpv) dna form i (supercoiled) was prepared from plantar warts. hpv dna was cleaved with restriction enzymes obtained from the following sources: escherichia coli (ecori), hemophilus influenzae strain rd (both unfractionated hind and aeparated hindii and hindiii enzymes) and hemophilus parainfluenzae (hpai). the cleavage products were analyzed by polyacrylamide gradient slab gel electrophoresis and electron microscopy. hpv dna was cleaved into two fragments by ecori (87% and ... | 1975 | 174077 |
| survival of human enteric and other sewage microorganisms under simulated deep-sea conditions. | the survival of pure cultures of escherichia coli, streptococcus faecalis, clostridium perfringens, and vibrio parahaemolyticus under simulated deep-sea conditions of low temperature (4 c), seawater, and hydrostatic pressures ranging from 1 to 1,000 atm was determined over a period exceeding 300 h. the viability of e. coli and total aerobic bacteria in seawater-diluted raw sewage subjected to these deep-sea conditions was also measured. there was a greater survival of both e. coli and s. faecali ... | 1975 | 169733 |
| sensitive assay for detection of toxin-induced damage to the cytoplasmic membrane of human diploid fibroblasts. | a sensitive assay was developed for detection and quantitation of subtle permeability changes in the cytoplasmic membrane of human diploid fibroblasts. release of the non-metabolizable amino acid [1-14c]alpha-aminoisobutyric acid (aib; molecular weight (103) from the cytoplasm of prelabeled cells was used as an indicator of toxin-induced membrane damage. an optimal procedure for labeling these cells was designed after varying the conditions with regard to ph, temperature, concentration of aib, c ... | 1975 | 169201 |
| bile composition and bile cast formation after transplantation of the liver in man. | transplantation of the liver in man is frequently complicated by biliary fistula and obstruction of the biliary tree by casts, which suggests that the composition of the bile may be abnormal. in part of the present study, bile composition was investigated in three recipients during the first few weeks after transplantation, when a t tube was in place. supersaturation of bile with cholesterol was found in two patients immediately after surgery and during episodes of acute rejection, but bile was ... | 1975 | 168674 |
| anatomy of herpes simplex virus dna: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. | digestion of herpes simplex virus dna by the hiniii or eco ri restriction endonucleases yielded 11 to 15 fragments with molecular weights between 1 x 10(6) and 28 x10(6). the electrophoretic profiles obtained in 0.3% agarose gels with dna fragments from none different strains of herpes simplex virus type 1 could be readily differentiated from the patterns exhibited by the corresponding fragments from four separate strains of type 2 virus; however, with each serotype, the laboratory strains diffe ... | 1975 | 168574 |
| effects of anaerobiosis and inhibitors on o2-production by human granulocytes. | a study was carried out on the effect of a number of inhibitors as well as of anaerobiosis on the production of superoxide (o2 minus) by human granulocytes. several metabolic inhibitors, including bazide, cyanide, antimycin a, and 2,4-dinitrophenol had no effect on o2 minus production. methimazole, which inhibits myeloperoxidase, was also without effect. the sulfhydryl reagents n-ethylmalemide and iodoacetamide greatly inhibited o2 minus production. o2 minus production did not take place under n ... | 1975 | 164965 |
| human growth hormone active site for membrane cooperative enzymes. | 1978 | 154327 | |
| the response to mitogens and allogeneic cells of human lymphocyte subpopulations separated by their differential binding to monolyaers of bacteria. | 1978 | 149175 | |
| feasibility of enterochelin as an iron-chelating drug: studies with human serum and a mouse model system. | 1978 | 149036 | |
| the occurrence of candida albicans in lake ontario bathing beaches. | there are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. through surveys of four bathing beaches on lake ontario, it was established that the opportunistically pathogenic yeast candida albicans occurs in near shore waters. the beaches surveyed could be differentiated on the basis of bacterial fecal ... | 1979 | 120220 |
| studies of ciliated epithelia of the human genital tract. 3: mucociliary wave activity in organ cultures of human fallopian tubes challenged with neisseria gonorrhoeae and gonococcal endotoxin. | quantative determinations of the mucociliary activity of human fallopian tube epithelium maintained as organ cultures were performed using a light beam reflex method. in non-infected organ cultures the mucociliary wave (mcw) frequency slowly decreased during the first 54 hours of culture maintenance. in organ cultures experimentally infected with fresh isolates of neisseria gonorrhoeae producing t1/t2 colonies the mcw frequency either decreased to subnormal values or completely ceased whereas in ... | 1979 | 114195 |
| gentic engineering for practical application. | genetic engineering has ushered in a new era in biology. although many problems are still to be solved, there are examples that point to a possible later application for the benefit of mankind: bacteria can be manipulated to degrade crude-oil spillages, to produce human insulin and to bind nitrogen from the air. if all the bacteria that are indigenous to agricultural soils could be made to bind nitrogen, an increase in soil fertility might well result. | 1979 | 108601 |
| frequency and fate of human renal allografts contaminated prior to transplantation. | bacterial cultures were done from donor ureter and/or graft perfusion fluid on 92 of 112 patients who had renal transplantations during 1977. three samples of perfusion fluid and 14 samples of donor ureter contained bacteria. in nine cases there was a gram-positive organism, and in 10 cases a gram-negative rod was (among) the contaminant(s). there was no difference in the rate of graft survival, patient survival, or in the frequency of major postoperative complications between the two groups. on ... | 1979 | 107605 |
| purification of heat-labile enterotoxin from four escherichia coli strains by affinity immunoadsorbent: evidence for similar subunit structure. | a single-step method for the purification of heat-labile enterotoxin of escherichia coli is described. the method involves an affinity immunoadsorbent made with antiserum to cholera toxin. crude toxin preparations of three human and one porcine enterotoxinogenic strains of e. coli were purified on this immunoadsorbent, and the elution products suggest that the toxin molecule is composed of subunits. one kind of subunit shared by these four strains showed similar mobility of sodium dodecyl sulfat ... | 1978 | 103838 |
| [urobilinogen excretion in the urine and bacterial overgrowth in the jejunum of patients with jejuno-or ileo-transversostomy in man (author's transl)]. | 1978 | 101703 | |
| effect of a staphylococcin on neisseria gonorrhoeae. | phage group 2 staphylococcal strain ut0002 contains a large 56s virulence plasmid with genes that code for both exfoliative toxin and a specific staphylococcin termed bac r(1). four penicillinase-producing strains and three penicillin-susceptible strains of neisseria gonorrhoeae were killed by bac r(1). after 30 min of growth of the penicillin-resistant tr1 strain in 62.5 arbitrary units of bac r(1) per ml, loss of viability was approximately 90%, and, after 5 h, an approximately 99.99% loss of ... | 1978 | 100049 |
| a human studying the sensing of chemicals by bacteria. | a new frame of reference, which in its fundamental structuring differs radically from the structuring of the familiar western indo-european viewpoints (logical, mathematical, scientific, philosophical, etc.), already exists. recently, by the strategem of systematically disallowing a previously unnoticed untenable assumption encoded in the traditional western symbolic logics, set theories, etc., in particular and in the western 'world-view' in general, this frame of reference has generated its ow ... | 1978 | 97887 |
| relationships among raffinose plasmids determined by the immunochemical cross-reaction of their alpha-galactosidases. | plasmid-encoded alpha-galactosidase served as a marker enzyme for the recognition and comparison of raffinose (raf) plasmids present in strains of escherichia coli. immunochemical relationships were established among raf plasmids of 39 independent isolates from man and domestic animals (from three continents) by using antiserum against alpha-galactosidase. immunodiffusion revealed three serological subclasses of alpha-galactosidase, which are correlated with the biological and geographical origi ... | 1979 | 94343 |
| secretory immunoglobulin a and g antibodies prevent adhesion of escherichia coli to human urinary tract epithelial cells. | the adhesion of escherichia coli to human urinary tract epithelial cells was inhibited by commercial gamma globulin, the total immunoglobulin fraction of human breast milk and urine, as well as the isolated immunoglobulin g and secretory immunoglobulin a fractions of urine from patients with acute pyelonephritis. urinary anti-o6 antibodies reduced the adhesion of several o6 strains. absorption of antibodies to the lipopolysaccharide of the adhering strain markedly decreased the antiadhesive capa ... | 1978 | 83303 |
| new surface-associated heat-labile colonization factor antigen (cfa/ii) produced by enterotoxigenic escherichia coli of serogroups o6 and o8. | enterotoxigenic escherichia coli (etec) belonging to serogroups o6 and o8 do not possess the h-10407-type colonization factor antigen (cfa/i). however, these frequently isolated etec were found to possess a second and distinct heat-labile surface-associated colonization factor antigen, termed cfa/ii. whereas cfa/i mediates mannose-resistant hemagglutination of human group a erythrocytes, cfa/ii does not. cfa/ii mediates mannose-resistant hemagglutination of bovine erythrocytes, and mannose-resis ... | 1978 | 80383 |
| host resistance to lipopolysaccharides in the pathogenesis of multiple sclerosis and membranoproliferative glomerulonephritis. | host resistance against bacterial lipopolysaccharides (l.p.s.) and especially against its toxic part lipid a has earlier been demonstrated in biological assays. in this paper an aryl-esterase is shown to be associated with alfa-1-lipoprotein (are) and is probably responsible for the detoxification of l.p.s. in man. furthermore c3 is shown to be activitated by l.p.s. from these facts it is suggested that are performs the initial degradation of l.p.s. followed by complement activation and trapping ... | 1976 | 73807 |
| differential staining of bacteria in clinical specimens using acridine orange buffered at low ph. | optimal conditions for acridine orange staining of air dried and methanol fixed bacteria on glass slides were studied. the ph of the staining buffer did not influence the fluorescence of an s. aureus and an e. coli strain at dye concentrations of 25-50 mg per litre. 81 bacterial strains representing 15 different species were stained with acridine orange under standard conditions, all strains showing orange fluorescence. the ph of the buffer influenced markedly the staining patterns of human cell ... | 1977 | 70957 |
| [protective properties of igm in experiments on animals]. | on a model of intraperitoneal infection of albino mice the authors demonstrated a protective action of the fraction enriched with igm and of gamma-globulin isolated from the normal human blood serum, against e. coli o111. the intensity of the protective action depended on the method, duration of administration of the preparation and also on the infective dose. protective properties of the fraction enriched with igm were more pronounced in comparison with the gamma-globulin preparation. | 1976 | 60856 |
| bacterial synthesis of substance similar to human chorionic gonadotrophin. | 1976 | 59931 | |
| modulation of regulatory mechanisms operative in the cyclical production of antibody. | modulation of the cyclical response in rabbits to aggregated human gamma globulin (ahuigg) was investigated in order to study some of the parameters involved in self-regulation of the immune response. several mitogens (lipopolysaccharide [lps], phytohemagglutinin [pha], and concanavalin a [con a]), when injected simultaneously with antigen, have been shown to modulate the normal splenic plaque-forming cell (pfc) response in rabbits to a single intravenous injection of ahuigg. this response to ah ... | 1976 | 55457 |
| mucosal adherence of human enteropathogenic escherichia coli. | an in-vitro assay system has been developed to measure bacterial adhesion to the mucosa of human fetal small intestine. two strains of escherichia coli that are proven human enteropathogens (e.p.e.c.) have been shown to adhere in large numbers, compared with control organisms. the attachment mechanism is species specific and is not caused by common fimbriae. mucosal adhesion may be as important as enterotoxin production or invasiveness in determining the virulence of some strains of human e.p.e. ... | 1975 | 53431 |
| cryopreservation of human granulocytes. | 1975 | 48447 | |
| urinary-tract infection: localisation and virulence of escherichia coli. | virulence of 15 strains of escherichia coli from the human upper urinary tract was compared with that of 16 strains from the lower urinary tract, using an ascending infection in the mouse. no significant difference was found. there was no significant difference in frequency of k antigen and ability to ferment dulcitol between 32 lower strains and 31 upper strains. however, 22 strains containing k antigen, regardless of anatomical site of localisation, were more significantly likely to cause infe ... | 1975 | 46051 |
| [an oral enteritis-vaccine composed of twelve heat inactivated enterobacteriaceae. 1. communication: theoretical and epidemiological considerations (author's transl)]. | the infectious diseases of the human intestinal tract which are caused by bacteria must be distinguished into two groups on account of their different pathogenesis: the cyclic infections (typhoid fever, parathyphoid fever) and the local infections (cholera, dysentery, salmonella enteritis, dyspepsia coli infections). the local infections of the intestine do not cause a systemic but only a local immunity of the intestinal mucosa. it is necessary therefore to induce local immunity as active immuno ... | 1979 | 44783 |
| opsonic activity of normal human cerebrospinal fluid for selected bacterial species. | the opsonic activity of normal human cerebrospinal fluid (csf) has not been well defined. in this study, the opsonic activity of normal csf for laboratory and blood culture isolates of staphylococcus aureus, streptococcus pneumoniae, escherichia coli, hemophilus influenzae type b, and neisseria meningitidis was measured by a quantitative assay employing radiolabeled bacteria and polymorphonuclear leukocytes. all isolates of s. aureus, except the wood 46 strain, were opsonized in undiluted csf (> ... | 1979 | 43289 |
| fimbrial colonization factor cfa/1 protein from human enteropathogenic escherichia coli strains. purification, characterization and n-terminal sequence. | 1979 | 42558 | |
| attachment pili from enterotoxigenic escherichia coli pathogenic for humans. | pili from enterotoxigenic escherichia coli pathogenic for humans have been isolated by adsorption to the surface of erythrocytes followed by thermal elution. the pili are composed of two protein subunits with molecular weights of 13,100 and 12,500 as determined by sodium dodecyl sulfate-gel electrophoresis. these pili also bind to human buccal cells under temperature conditions (37 degrees c) which prevent the binding of these pili to the erythrocytes. analogous temperature effects on binding ha ... | 1979 | 40882 |
| production of heat-stable, methanol-soluble enterotoxin by yersinia enterocolitica. | seven isolates of yersinia enterocolitica serotype 0:8, recovered during an outbreak of gastrointestinal illness, were examined for enterotoxin production. all seven strains were enterotoxigenic in the suckling mouse model, and three of five isolates tested produced keratoconjunctivitis in the guinea pig eye model (sereny test). enterotoxin was detected in broth supernatant fluid after 12 h of incubation at 25 degrees c. the toxin was not inactivated by exposure to 121 degrees c for 30 min or by ... | 1979 | 39891 |
| adherence of escherichia coli to human urinary tract epithelial cells. | the adherence of escherichia coli to human uroepithelial cells obtained from midstream urine specimens of healthy women was studied. bacteria labeled with [(3)h]uridine were used, and unattached organisms were separated from the epithelial cells by vacuum filtration with 5-mum-pore-size nucleopore membrane filters. these techniques allowed adherence to be measured in large numbers of epithelial cells and overcame the problem of distinguishing experimental bacteria from the indigenous organisms p ... | 1979 | 38207 |
| the prerequisites for local lysolecithin formation in the human gallbladder. iii. demonstration of two different phospholipase a activities. | the positional specificity of the phospholipase a (ec 3.1.1.4) in human gallbladder epithelium has been studied using 14c-phosphatidylethanolamine radiolabeled either in the 1-acyl or in the 2-acyl position. after heating of homogenized epithelial cells at 70 degrees c for 2 min, their lysophospholipase activity was lost. in contrast, the ability to hydrolyze 14c-phosphatidylethanolamine in biosynthetically radiolabeled escherichia coli was largely retained. the amounts of radioactivity found i ... | 1979 | 35826 |
| some effects of the administration of endotoxin in mice. specific cleavage of serum albumin by an acid protease and the generation of amyloid serum component. | endotoxin has been shown to induce amyloidosis in mice and to result in the appearance in serum of large amounts of amyloidrelated protein (saa). after injection of 300 mug lipopolysaccharide escherichia coli, saa behaves as an acute phase reactant with levels reaching a peak of >600 mug/ml at 18-22 h and returning to base line (<50 mug/ml) by 48 h in each of four strains tested; only the endotoxin-resistant c3h/hej strain showed a smaller response. lesser, though significant, elevations were al ... | 1979 | 34628 |
| nutritional requirements for synthesis of heat-labile enterotoxin by enterotoxigenic strains of escherichia coli. | optimal growth conditions have been established for production of heat-labile enterotoxin (lt) by both porcine and human strains of enterotoxigenic (ent(+)) escherichia coli. there were no unusual growth factor requirements, and some strains produced fairly high levels of lt in a basal salts medium containing 0.5% glucose if the ph was carefully controlled. several amino acids markedly stimulated lt synthesis when added to the basal salts-glucose medium. methionine and lysine were the most stimu ... | 1979 | 33900 |
| stereoelectronic factors in the binding of substrate analogues and inhibitors to purine nucleoside phosphorylase isolated from human erythrocytes. | several aspects of the stereoelectronic requirements of substrates of human erythrocytic purine nucleoside phosphorylase (e.c. 2.4.2.1) were elucidated providing the following information: (a) the n1 position cannot have a nonhydrogen substituent; (b) the 5'-oh group must be present for catalytic activity to be exhibited but is not an essential functional group for inhibitory action to be observed; (c) on the c8 position groups larger than -nh2 or -br cannot be accommodated; (d) the syn-glycosyl ... | 1978 | 31484 |
| 21-dehydroxylation of corticoids by anaerobic bacteria isolated from human fecal flora. | 1978 | 28451 | |
| evidence for quantitative variability of bacterial opsonic requirements. | we studied human serum opsonins by using combinations of heat inactivation and chelation to inhibit complement, adsorption to remove antibody, and trypan blue to inactivate the c3 receptor of human polymorphonuclear leukocytes. streptococcus pneumoniae, serotype 25, required both complement and immunoglobulin for opsonization, even though that strain activated the alternative complement pathway. both strains of escherichia coli required antibody and complement, but varied in the degree of depend ... | 1978 | 25243 |
| adhesion of escherichia coli to human uroepithelial cells in vitro. | optimal conditions for in vitro adherence of escherichia coli to uroepithelial cells, previously shown to more efficient for strains causing acute symptomatic than that for strains causing "asymptomatic" urinary tract infections, were investigated. uroepithelial cells from fresh morning urine of healthy individuals and e. coli bacteria from patients with various forms of urinary tract infeciton were used. adhesion was found to vary, between individuals and epithelial cell types, with epithelial ... | 1977 | 22493 |
| determinants of lung bacterial clearance in mice after acute hypoxia. | net lung bacterial clearance in normal mice is determined by the balance of in vivo bacterial multiplication on the one hand, and the defense mechanisms of mucociliary clearance and phagocytosis and killing by the oxygen-dependent alveolar macrophage on the other. the bactericidal function of the macrophage is the major component of the defense mechanism. the effect of acute hypoxia on the defense mechanism was studied in mice exposed to aerosols of staphylococcus aureus, escherichia coli, klebs ... | 1977 | 21603 |
| formation of mutagenic n-nitroso compounds from the pesticides prometryne, dodine and carbaryl in the presence of nitrite at ph 1. | environmental chemicals including pesticides carrying secondary and tertiary amino groups are suggested to be a health hazard to man since potentially carcinogenic nitroso compounds may be formed in the presence of nitrite at low ph values resembling conditions in the human stomach. nitrosation of the isopropylamino-triazine prometryne, the n-dodecyl guanidine dodine and the n-methylcarbamate carbaryl was investigated in the presence of hcl and acetic acid at ph 1 and excess sodium nitrite for 4 ... | 1976 | 12468 |
| microbicidal mechanisms of human granulocytes: synergistic effects of granulocyte elastase and myeloperoxidase or chymotrypsin-like cationic protein. | the antibacterial activity of a myeloperoxidase (mpo)-glucose oxidase system was found to be greatly increased by granulocyte elastase, present in azurophil granules of human neutrophils. the mpo-h2o3-mediated killing of both escherichia coli and staphylococcus aureus was potentiated by granuocyte elastase at an acid ph, whereas at ph 7.4 only killing of e. coli was potentiated. the potentiating effect of elastase was not dependent on the enzymatic properties of the protein since it was not abol ... | 1976 | 12111 |
| some properties of the alpha-hemolysin produced by a hemolytic strain of e. coli. | it was found that alpha-hemolysin of e. coli p 678 hiy+ was maximally active against human erythrocytes at ph 6.5. the hemolytic activity is characterized in time by a distinct lag-phase and a phase of the greatest velocity of the reaction immediately following it. the duration of the lag-phase and also the rate of hemolysis depends on alpha-hemolysin concentration, whose increase is accompanied by a decrease of the lag-phase and acceleration of hemolysis. there is a definite limit below which t ... | 1975 | 1949 |
| microbiol growth in lipid emulsions used in parenteral nutrition. | parenteral nutrition via central venous catheterization is associated with serious risks, especially that of sepsis. lipid emulsion (intralipidsweden), which may be administered peripherally, was evaluated for its potential to support microbial growth. washed cultures of staphylococcus aureus, candida albicans, and three species of gram-negative rods were all capable of multiplying in the emulsion at room temperature. variations in inoculum size did not affect the growth rate. studies comparing ... | 1975 | 982 |