Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| use of an open-reading frame-specific campylobacter jejuni dna microarray as a new genotyping tool for studying epidemiologically related isolates. | findings from use of an open-reading frame-specific campylobacter jejuni dna microarray to investigate genetic diversity among clinical isolates associated with 5 independent clusters of infection were compared with data from random amplified polymeric dna (rapd) and penner serotyping analyses. the dna microarray provides a highly specific epidemiological typing tool for analysis of c. jejuni isolates and reveals both divergent and highly conserved gene classes among isolates. | 2003 | 12599089 |
| determination of ciprofloxacin and nalidixic acid resistance in campylobacter jejuni with a fluorogenic polymerase chain reaction assay. | a fluorogenic polymerase chain reaction assay for the gyra gene was used to determine the frequency of a thr-86 mutation in campylobacter jejuni isolates from food animals and humans in northern thailand and to investigate the correlation between this mutation and bacterial resistance to fluoroquinolones. eighty-four isolates of c. jejuni were used: 65 from healthy chickens on farms, 16 from chickens at the slaughterhouse, 1 from chicken meat at the market, and 1 from a healthy farm worker. the ... | 2003 | 12597495 |
| detecting adverse events using information technology. | although patient safety is a major problem, most health care organizations rely on spontaneous reporting, which detects only a small minority of adverse events. as a result, problems with safety have remained hidden. chart review can detect adverse events in research settings, but it is too expensive for routine use. information technology techniques can detect some adverse events in a timely and cost-effective way, in some cases early enough to prevent patient harm. | 2003 | 12595401 |
| rpod promoters in campylobacter jejuni exhibit a strong periodic signal instead of a -35 box. | we have used a hidden markov model (hmm) to identify the consensus sequence of the rpod promoters in the genome of campylobacter jejuni. the identified promoter consensus sequence is unusual compared to other bacteria, in that the region upstream of the tata-box does not contain a conserved -35 region, but shows a very strong periodic variation in the at-content and semi-conserved t-stretches, with a period of 10-11 nucleotides. the tata-box is in some, but not all cases, preceded by a tgx, simi ... | 2003 | 12595250 |
| occurrence and resistance to antibiotics of campylobacter jejuni and campylobacter coli in animals and meat in northeastern italy. | a study was carried out in northeastern italy during 2000 and 2001 to investigate the occurrence of campylobacter jejuni and campylobacter coli in animals, cattle, pigs, and broilers, and raw meat, beef, pork, and chicken. campylobacter spp. were detected in 53.9% of the cattle, 63.5% of the pigs, and 82.9% of the broilers examined. chicken meat was frequently contaminated (81.3%), while lower rates were found in pork meat (10.3%) and beef (1.3%). the resistance to antibiotics of the strains was ... | 2003 | 12593931 |
| intravenous immunoglobulin in the treatment of primary trigeminal neuralgia refractory to carbamazepine: a study protocol [isrctn33042138]. | we have recently reported successful treatment of patients with chronic pain syndromes using human pooled intravenous immunoglobulin (ivig) in a prospective, open-label cohort study. a randomised, placebo controlled, double blinded study is needed to confirm these results. we chose to study patients with carbamazepine resistant primary trigeminal neuralgia (rptn), as these had responded particularly well to ivig.a protocol involving the use of ivig in rptn is complex for three reasons: 1. the ef ... | 2003 | 12590652 |
| barbecued chicken causing a multi-state outbreak of campylobacter jejuni enteritis. | although the microbiological safety of food has improved, food-borne disease remains a significant cause of morbidity and mortality in europe. | 2003 | 12590328 |
| the genetics of glycosylation in gram-negative bacteria. | in recent years there has been a dramatic increase in reports of glycosylation of proteins in various gram-negative systems including neisseria meningitidis, neisseria gonorrhoeae, campylobacter jejuni, pseudomonas aeruginosa, escherichia coli, caulobacter crescentus, aeromonas caviae and helicobacter pylori. although this growing list contains many important pathogens (reviewed by benz and schmidt [mol. microbiol. 45 (2002) 267-276]) and the glycosylations are found on proteins important in pat ... | 2003 | 12586395 |
| intestinal epithelial responses to enteric pathogens: effects on the tight junction barrier, ion transport, and inflammation. | the effects of pathogenic organisms on host intestinal epithelial cells are vast. innumerable signalling pathways are triggered leading ultimately to drastic changes in physiological functions. here, the ways in which enteric bacterial pathogens utilise and impact on the three major physiological functions of the intestinal epithelium are discussed: alterations in the structure and function of the tight junction barrier, induction of fluid and electrolyte secretion, and activation of the inflamm ... | 2003 | 12584232 |
| maximal adherence and invasion of int 407 cells by campylobacter jejuni requires the cadf outer-membrane protein and microfilament reorganization. | the binding of campylobacter jejuni to fibronectin (fn), a component of the extracellular matrix, is mediated by a 37 kda outer-membrane protein termed cadf for campylobacter adhesion to fibronectin. the specificity of c. jejuni binding to fn, via cadf, was demonstrated using antibodies reactive against fn and cadf. more specifically, the anti-cadf antibody reduced the binding of two c. jejuni clinical isolates to immobilized fn by greater than 50 %. furthermore, a c. jejuni wild-type isolate, i ... | 2003 | 12576589 |
| development of a multilocus sequence typing method for analysis of listeria monocytogenes clones. | this study is a first step in the development of multilocus sequence typing (mlst) method for listeria monocytogenes. nine housekeeping genes were analyzed in a set of 62 strains isolated from different sources and geographic locations in spain. these strains were previously characterized by pulsed-field gel electrophoresis (pfge). because of low diversity, two loci were discarded from the study. the sequence analysis of the seven remaining genes showed 29 different allelic combinations, with 22 ... | 2003 | 12574278 |
| mechanism of tet(o)-mediated tetracycline resistance. | tet(o) is an elongation factor-like protein which confers resistance to the protein synthesis inhibitor tetracycline by promoting the release of the drug from its inhibitory site on the ribosome. here we investigated the interaction of tet(o) with the elongating ribosome and show, using dimethyl sulfate (dms) probing and binding assays, that it interacts preferentially with the post-translocational ribosome. furthermore, using an xtp-dependent mutant of tet(o), we demonstrated that tet(o) induce ... | 2003 | 12574130 |
| antimicrobial susceptibility testing of campylobacter jejuni: a comparison between etest and agar dilution method. | the susceptibility of campylobacter jejuni strains (n = 50) against nine antimicrobials were determined in comparison with etest (ab biodisk, solna, sweden) and agar dilution method to further investigate the correlation between the two methods. all the strains were isolated from stool samples of patients with diarrhea in 1998 and found to be highly susceptible (>84%) to ampicillin, tetracycline, gentamicin, chloramphenicol, ciprofloxacin and erythromycin. the essential agreement between two met ... | 2003 | 12573553 |
| characterization of a new operon, as-48efgh, from the as-48 gene cluster involved in immunity to enterocin as-48. | enterocin as-48 is a cyclic peptide produced by enterococcus faecalis s-48 whose genetic determinants have been identified in the conjugative plasmid pmb2. a region of 7.8 kb, carrying the minimum information required for production of and immunity against as-48, had been previously cloned and sequenced in pam401 (pam401-52). in this region, the as-48a structural gene and as-48b, as-48c, as-48c(1), as-48d, and as-48d(1) genes and open reading frame 6 (orf6) and orf7 had been identified. the sequ ... | 2003 | 12571051 |
| specific detection of arcobacter and campylobacter strains in water and sewage by pcr and fluorescent in situ hybridization. | the aim of this study was to evaluate pcr and fluorescent in situ hybridization (fish) techniques for detecting arcobacter and campylobacter strains in river water and wastewater samples. both 16s and 23s rrna sequence data were used to design specific primers and oligonucleotide probes for pcr and fish analyses, respectively. in order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on ... | 2003 | 12571045 |
| evaluation of broiler litter with reference to the microbial composition as assessed by using 16s rrna and functional gene markers. | very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. we evaluated the bacterial composition of poultry litter by using a combination of culture and molecular detection. total aerobic bacteria in poultry litter were detected by culture at 10(9) cfu/g of material. enteric bacteria such as enteroco ... | 2003 | 12571010 |
| evolutionary implications of microbial genome tetranucleotide frequency biases. | we compared nucleotide usage pattern conservation for related prokaryotes by examining the representation of dna tetranucleotide combinations in 27 representative microbial genomes. for each of the organisms studied, tetranucleotide usage departures from expectations (tud) were shared between related organisms using both markov chain analysis and a zero-order markov method. individual strains, multiple chromosomes, plasmids, and bacteriophages share tuds within a species. tuds varied between cod ... | 2003 | 12566393 |
| prokaryotic utilization of the twin-arginine translocation pathway: a genomic survey. | the twin-arginine translocation (tat) pathway, which has been identified in plant chloroplasts and prokaryotes, allows for the secretion of folded proteins. however, the extent to which this pathway is used among the prokaryotes is not known. by using a genomic approach, a comprehensive list of putative tat substrates for 84 diverse prokaryotes was established. strikingly, the results indicate that the tat pathway is utilized to highly varying extents. furthermore, while many prokaryotes use thi ... | 2003 | 12562823 |
| campylobacter jejuni binds intestinal h(o) antigen (fuc alpha 1, 2gal beta 1, 4glcnac), and fucosyloligosaccharides of human milk inhibit its binding and infection. | the most common cause of infant mortality is diarrhea; the most common cause of bacterial diarrhea is campylobacter jejuni, which is also the primary cause of motor neuron paralysis. the first step in campylobacter pathogenesis is adherence to intestinal mucosa. we found that such binding was inhibited in vitro by human milk and, with high avidity, by alpha1,2-fucosylated carbohydrate moieties containing the h(o) blood group epitope (fuc alpha 1,2gal beta 1,4glcnac em leader ). in studies on the ... | 2003 | 12562767 |
| high resolution genotyping of campylobacter jejuni strains by macrorestriction analysis with xhoi and polymerase chain reaction targeting enterobacterial repetitive intergenic consensus sequences: can we predict the zoonotic potential of strains? | campylobacter jejuni isolates of human, canine, feline, bovine and poultry origin were investigated for their genomic diversity using o-antigen typing (n = 271), smai (n = 158) and xhoi (n = 158) macrorestriction analysis and eric-pcr (n = 107). the o-antigens o:1/44, o:2, o:4 complex, o:37. o:40 were identified and 53.7% of the human and 56.1% of the animal strains were typable with the available antisera. two eric-pcr pattern groups were generated representing human and animal strains as well ... | 2002 | 12558325 |
| enhancement of disease and pathology by synergy of trichuris suis and campylobacter jejuni in the colon of immunologically naive swine. | campylobacter jejuni, a leading cause of bacterial gastroenteritis, has different age distribution and disease expression in developing and developed countries, which may be due to the endemnicity of infection and the age of acquisition of immunity. differences in disease expression are not solely dependent on the c jejuni strain or virulence attributes. another modulating factor in developing countries may be endemic nematode infections such as trichuris, which drive type 2 cytokine responses a ... | 2003 | 12556152 |
| elucidation of trna-dependent editing by a class ii trna synthetase and significance for cell viability. | editing of misactivated amino acids by class i trna synthetases is encoded by a specialized internal domain specific to class i enzymes. in contrast, little is known about editing activities of the structurally distinct class ii enzymes. here we show that the class ii alanyl-trna synthetase (alars) has a specialized internal domain that appears weakly related to an appended domain of threonyl-trna synthetase (thrrs), but is unrelated to that found in class i enzymes. editing of misactivated glyc ... | 2003 | 12554667 |
| the health status of newly arrived refugee children in miami-dade county, florida. | 2003 | 12554586 | |
| evolutionary connection between the catalytic subunits of dna-dependent rna polymerases and eukaryotic rna-dependent rna polymerases and the origin of rna polymerases. | the eukaryotic rna-dependent rna polymerase (rdrp) is involved in the amplification of regulatory micrornas during post-transcriptional gene silencing. this enzyme is highly conserved in most eukaryotes but is missing in archaea and bacteria. no evolutionary relationship between rdrp and other polymerases has been reported so far, hence the origin of this eukaryote-specific polymerase remains a mystery. | 2003 | 12553882 |
| campylobacter transmission in a peruvian shantytown: a longitudinal study using strain typing of campylobacter isolates from chickens and humans in household clusters. | campylobacter jejuni is a major cause of pediatric diarrhea in developing countries-free-ranging chickens are presumed to be a common source. campylobacter strains from monthly surveillance and diarrhea cases were compared by means of restriction-fragment length polymorphism (rflp), rapid amplified polymorphic dna, and lior serotyping. rflp analysis of 156 human and 682 avian strains demonstrated identical strains in chickens and humans in 29 (70.7%) of 41 families, and 35%-39% of human isolates ... | 2003 | 12552450 |
| two hsp70 family members expressed in atherosclerotic lesions. | gene expression profiling was carried out comparing con a elicited peritoneal macrophages from c57bl6 and fvbn wild-type and apolipoprotein (apo)e knockout mice. an est, was expressed at higher levels in c57bl6 compared with fvbn mice. mapped to an atherosclerosis susceptibility locus on chromosome 19 revealed in an intercross between atherosclerosis-susceptible c57bl6 and atherosclerosis-resistant fvbn apoe knockout mice. a combination of database search and northern analysis confirmed that cor ... | 2003 | 12552099 |
| prevalence of enteric pathogens in dogs of north-central colorado. | to evaluate the prevalence of enteric pathogens in dogs of north-central colorado, fecal samples were obtained from client-owned dogs presented to the veterinary teaching hospital at colorado state university for evaluation of acute small-bowel, large-bowel, or mixed-bowel diarrhea (n=71) and from age-matched, client-owned, healthy dogs (n=59). infectious agents potentially associated with gastrointestinal disease were detected in 34 of 130 (26.1%) fecal samples. agents with zoonotic potential w ... | 2003 | 12549614 |
| [study on the physicochemical properties of campylobacter jejuni enterotoxin]. | precipitate of campylobacter jejuni cytotonic enterotoxin(ce) performed in an 80% saturated solution of ammonium sulfateit indicated that there were some little molecular proteins except the 68 kd main band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis(sds-page), whereas the eluate from gm1 ganglioside affinity column chromatography exhibited only one 68 kd band on sds-page. the results suggest that ce mainly be consisted of 68 kd protein. the toxin is heat-labile, ph dependent an ... | 2000 | 12548883 |
| [some differences on biological characteristics of campylobacter jejuni]. | fecal samples from 195 diarrheic patients in different age, rectal and cloacal swabs from 656 normal and diarrheic animals and poultry, 108 visceral materials from the dead animals with diarrhoea were cultured for compylobacter jejuni. a total of 458 strains of campylobacters (445 strains of c. jejuni, 13 strains of c. coli) were isolated and identified, and some biological characteristics of these strains were observed. lior's biotyping scheme was used for biotyping 354 strains of campylobacter ... | 2000 | 12548753 |
| bacterial autoinduction: looking outside the cell for new metabolic engineering targets. | recent evidence has demonstrated that cell-to-cell signaling is a fundamental activity carried out by numerous microorganisms. a number of specialized processes are reported to be regulated by density-dependent signaling molecules including antibiotic production, bioluminescence, biofilm formation, genetic competence, sporulation, swarming motility and virulence. however, a more centralized role for quorum sensing is emerging where quorum signaling pathways overlap with stress and starvation cir ... | 2002 | 12537600 |
| reference isolates for the clonal complexes of campylobacter jejuni. | to identify and make available through the national collection of type cultures (nctc) a set of reference isolates for the clonal complexes of campylobacter jejuni. | 2003 | 12535131 |
| the iron-binding protein dps confers hydrogen peroxide stress resistance to campylobacter jejuni. | we identified and characterized the iron-binding protein dps from campylobacter jejuni. electron microscopic analysis of this protein revealed a spherical structure of 8.5 nm in diameter, with an electron-dense core similar to those of other proteins of the dps (dna-binding protein from starved cells) family. cloning and sequencing of the dps-encoding gene (dps) revealed that a 450-bp open reading frame (orf) encoded a protein of 150 amino acids with a calculated molecular mass of 17,332 da. ami ... | 2003 | 12533477 |
| rates and consequences of recombination between rrna operons. | a mutant strain of escherichia coli was created by inserting a cassette encoding sucrose sensitivity and neomycin resistance (sacb-neo) into the small-subunit rrna-encoding gene rrs in the rrnb operon. during growth in a complex medium, the cassette was lost from the population, and a complete rrs gene was restored at a rate of 5 x 10(-9) per cell division. repair of this lesion required flanking regions of dna that were similar to the six remaining intact rrna operons and reestablished the full ... | 2003 | 12533472 |
| application of single-strand conformation polymorphism and denaturing gradient gel electrophoresis for fla sequence typing of campylobacter jejuni. | campylobacter jejuni is a frequent cause of bacterial gastroenteritis in humans all over the world. several molecular typing methods are used to study the epidemiology of campylobacter spp. infections. the aim of the present study was to investigate the application of single-strand conformation polymorphism (sscp) and denaturing gradient gel electrophoresis (dgge) analysis as rapid primary subtyping methods for c. jejuni. a variable fragment from the 3' end of the flaa to the 3' end of the inter ... | 2003 | 12531499 |
| noti passporting to identify species composition of complex microbial systems. | we describe here a new method for large-scale scanning of microbial genomes on a quantitative and qualitative basis. to achieve this aim we propose to create noti passports: databases containing noti tags. we demonstrated that these tags comprising 19 bp of sequence information could be successfully generated using dna isolated from intestinal or fecal samples. such noti passports allow the discrimination between closely related bacterial species and even strains. this procedure for generating r ... | 2003 | 12527794 |
| colonisation phenotype and colonisation potential differences in campylobacter jejuni strains in chickens before and after passage in vivo. | campylobacter jejuni strains isolated from chicken faeces and from humans suffering from gastroenteritis were used to determine the colonisation phenotypes and colonisation potential of these strains in chickens. five different colonisation types were observed ranging from immediate and sustained colonisation to completely non-colonising. phenotype one showed immediate colonisation with prolonged excretion of viable c. jejuni bacteria. phenotype two showed delayed colonisation with prolonged exc ... | 2003 | 12523984 |
| selection and characterization of fluoroquinolone-resistant mutants of campylobacter jejuni using enrofloxacin. | significant levels of fluoroquinolone resistance were obtained in campylobacterjejuni isolates after an unique step of selection using enrofloxacin. an asp90-to-asn and a thr86-to-ile change in the gyrase subunit gyra were found associated with a low (mic < or = 8 /microg/ml) or a high (mic > or = 16 microg/ml) level of resistance to ciprofloxacin, respectively. an association of both mutations conferred a higher level of resistance (mic > or = 128 microg/ml). further steps of selection increase ... | 2002 | 12523631 |
| reductive genome evolution in buchnera aphidicola. | we have sequenced the genome of the intracellular symbiont buchnera aphidicola from the aphid baizongia pistacea. this strain diverged 80-150 million years ago from the common ancestor of two previously sequenced buchnera strains. here, a field-collected, nonclonal sample of insects was used as source material for laboratory procedures. as a consequence, the genome assembly unveiled intrapopulational variation, consisting of approximately 1,200 polymorphic sites. comparison of the 618-kb (kbp) g ... | 2003 | 12522265 |
| enlarged famsbase: protein 3d structure models of genome sequences for 41 species. | enlarged famsbase is a relational database of comparative protein structure models for the whole genome of 41 species, presented in the gtop database. the models are calculated by full automatic modeling system (fams). enlarged famsbase provides a wide range of query keys, such as name of orf (open reading frame), orf keywords, protein data bank (pdb) id, pdb heterogen atoms and sequence similarity. heterogen atoms in pdb include cofactors, ligands and other factors that interact with proteins, ... | 2003 | 12520053 |
| pep: predictions for entire proteomes. | pep is a database of predictions for entire proteomes. the database contains summaries of analyses of protein sequences from a range of organisms representing all three major kingdoms of life: eukaryotes, prokaryotes and archaea. all proteins publicly available for organisms were aligned against swiss-prot, trembl and pdb. additionally, the following annotations are provided: secondary structure, transmembrane helices, coiled coils, regions of low complexity, signal peptides, prosite motifs, nuc ... | 2003 | 12520036 |
| hgt-db: a database of putative horizontally transferred genes in prokaryotic complete genomes. | the horizontal gene transfer database (hgt-db) is a genomic database that includes statistical parameters such as g+c content, codon and amino-acid usage, as well as information about which genes deviate in these parameters for prokaryotic complete genomes. under the hypothesis that genes from distantly related species have different nucleotide compositions, these deviated genes may have been acquired by horizontal gene transfer. the current version of the database contains 88 bacterial and arch ... | 2003 | 12519978 |
| epidemiology and antimicrobial susceptibilities of 111 campylobacter fetus subsp. fetus strains isolated in québec, canada, from 1983 to 2000. | the epidemiology and antimicrobial susceptibilities of 111 campylobacter fetus subsp. fetus strains isolated from 103 patients from 1983 to 2000 in québec, canada, were determined. the median number of patients infected annually with this bacteria was seven, with an incidence of 0.1 per 100,000 population. the male-to-female ratio was 1.1 to 1.0. the patients originated from 13 of the 18 québec socioeconomic regions. the age range of the patients was 6 months to 90 years old, 53% being > or = 70 ... | 2003 | 12517895 |
| real-time pcr assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in helicobacter pylori. | the main cause of failure of helicobacter pylori eradication therapy is resistance to clarithromycin. the resistance is due to three point mutations in two positions on the 23s rrna (a2142c, a2142g, and a2143g). our aim was to develop a rapid and accurate method to detect these mutations directly on biopsy specimens. we developed a real-time pcr that included a simultaneous detection of the amplicons by hybridization of two probes labeled with lc-red and fluorescein by using the fluorescence res ... | 2003 | 12517879 |
| evaluation of 11 pcr assays for species-level identification of campylobacter jejuni and campylobacter coli. | we examined the sensitivity and specificity of 11 pcr assays described for the species identification of campylobacter jejuni and campylobacter coli by using 111 type, reference, and field strains of c. jejuni, c. coli, and campylobacter lari. for six assays, an additional 21 type strains representing related campylobacter, arcobacter, and helicobacter species were also included. pcr tests were initially established in the laboratory by optimizing conditions with respect to five type and referen ... | 2003 | 12517869 |
| comparison of lightcycler pcr, rapid antigen immunoassay, and culture for detection of group a streptococci from throat swabs. | we compared the performance characteristics of a real-time pcr method, the lightcycler strep-a assay (roche applied science, indianapolis, ind.), to those of a rapid antigen immunoassay, the directigen 1-2-3 group a strep test kit (bd diagnostic systems, sparks, md.), and a standard culture method for detection of group a streptococci (gas) from 384 throat swabs. the lightcycler pcr produced more positive results (n = 58) than either culture (n = 55) or the directigen immunoassay (n = 31). the r ... | 2003 | 12517855 |
| genetic diversity of neisseria gonorrhoeae housekeeping genes. | molecular typing of neisseria gonorrhoeae strains is an important tool for epidemiological studies of gonococcal infection and transmission. the recently developed multilocus sequence typing (mlst) method is based on the genetic variation among housekeeping genes. as a preliminary investigation for the development of such a method, we characterized the genetic diversity at 18 gonococcal housekeeping gene loci. approximately 17,500 nucleotides, spanning 18 loci, were sequenced from 24 isolates. i ... | 2003 | 12517848 |
| detection, isolation, and molecular subtyping of escherichia coli o157:h7 and campylobacter jejuni associated with a large waterborne outbreak. | the largest reported outbreak of waterborne escherichia coli o157:h7 in the united states occurred in upstate new york following a county fair in august 1999. culture methods were used to isolate e. coli o157:h7 from specimens from 128 of 775 patients with suspected infections. campylobacter jejuni was also isolated from stools of 44 persons who developed diarrheal illness after attending this fair. there was one case of a confirmed coinfection with e. coli o157:h7 and c. jejuni. molecular detec ... | 2003 | 12517844 |
| comparative genotyping of campylobacter jejuni by amplified fragment length polymorphism, multilocus sequence typing, and short repeat sequencing: strain diversity, host range, and recombination. | three molecular typing methods were used to study the relationships among 184 campylobacter strains isolated from humans, cattle, and chickens. all strains were genotyped by amplified fragment length polymorphism (aflp) analysis, multilocus sequence typing (mlst), and sequence analysis of a genomic region with short tandem repeats designated clustered regularly interspaced short palindromic repeats (crisprs). mlst and aflp analysis yielded more than 100 different profiles and patterns, respectiv ... | 2003 | 12517820 |
| no simple dependence between protein evolution rate and the number of protein-protein interactions: only the most prolific interactors tend to evolve slowly. | it has been suggested that rates of protein evolution are influenced, to a great extent, by the proportion of amino acid residues that are directly involved in protein function. in agreement with this hypothesis, recent work has shown a negative correlation between evolutionary rates and the number of protein-protein interactions. however, the extent to which the number of protein-protein interactions influences evolutionary rates remains unclear. here, we address this question at several differ ... | 2003 | 12515583 |
| algorithms for computing parsimonious evolutionary scenarios for genome evolution, the last universal common ancestor and dominance of horizontal gene transfer in the evolution of prokaryotes. | comparative analysis of sequenced genomes reveals numerous instances of apparent horizontal gene transfer (hgt), at least in prokaryotes, and indicates that lineage-specific gene loss might have been even more common in evolution. this complicates the notion of a species tree, which needs to be re-interpreted as a prevailing evolutionary trend, rather than the full depiction of evolution, and makes reconstruction of ancestral genomes a non-trivial task. | 2003 | 12515582 |
| survival and resuscitation of ten strains of campylobacter jejuni and campylobacter coli under acid conditions. | the culturability of 10 strains of campylobacter jejuni and campylobacter coli was studied after the bacteria were exposed to acid conditions for various periods of time. campylobacter cells could not survive 2 h under acid conditions (formic acid at ph 4). the 10 campylobacter strains could not be recovered, even when enrichment media were used. viable cells, however, could be detected by a double-staining (5-cyano-2,3-ditolyl tetrazolium chloride [ctc]-4',6'-diamidino-2-phenylindole [dapi]) te ... | 2003 | 12514068 |
| investigation on the inhibition of campylobacter jejuni growth with the applications of some preservatives, medicines, herbs and herb preparations. | the aim of this study was to determine the influence of chosen preservatives, medicines, herbs and herb preparations on 39 campylobacter jejuni strains, which were isolated from fresh poultry carcasses. de-nol and ventrisol were used in the original concentration. propolis, propolan, artecholin and aukalen were of pharmacies origin. the rest of substances examined were 10% water solutions. out of 11 substances examined, potassium sorbate, sodium benzoate, de-nol and ventrisol exerted the stronge ... | 2002 | 12512554 |
| detection of campylobacter jejuni in healthy monkeys and monkeys with enteric infections by pcr. | campylobacter were detected by pcr in feces of monkeys of different species (clinically healthy, with diarrhea, and dead from acute enteric infections). high prevalence of these bacteria in monkeys was revealed. the incidence of c. jejuni dna in monkeys with acute enteric infections was higher than in healthy animals (69.6 and 51.3%, respectively). the highest percentage (92.3) of positive results was observed in macaca mulatta with enteric diseases and in macaque dead of these diseases. the pre ... | 2002 | 12512007 |
| flagella and motility in actinobacillus pleuropneumoniae. | actinobacillus pleuropneumoniae has been considered nonmotile and nonflagellate. in this work, it is demonstrated that a. pleuropneumoniae produces flagella composed of a 65-kda protein with an n-terminal amino acid sequence that shows 100% identity with those of escherichia coli, salmonella, and shigella flagellins. the dna sequence obtained through pcr of the flic gene in a. pleuropneumoniae showed considerable identity (93%) in its 5' and 3' ends with the dna sequences of corresponding genes ... | 2003 | 12511514 |
| enhanced surveillance of campylobacter infection in the north west of england 1997-1999. | to identify sources and routes of infection for sporadic cases of campylobacter infection in the north west of england. | 2003 | 12504607 |
| whole-proteome interaction mining. | motivation: a major post-genomic scientific and technological pursuit is to describe the functions performed by the proteins encoded by the genome. one strategy is to first identify the protein-protein interactions in a proteome, then determine pathways and overall structure relating these interactions, and finally to statistically infer functional roles of individual proteins. although huge amounts of genomic data are at hand, current experimental protein interaction assays must overcome techni ... | 2003 | 12499302 |
| in vivo selection of campylobacter isolates with high levels of fluoroquinolone resistance associated with gyra mutations and the function of the cmeabc efflux pump. | enrofloxacin treatment of chickens infected with fluoroquinolone(fq)-sensitive campylobacter promoted the emergence of fq-resistant campylobacter mutants which propagated in the intestinal tract and recolonized the chickens. the recovered isolates were highly resistant to quinolone antibiotics but remained susceptible to non-fq antimicrobial agents. specific single-point mutations in the gyra gene and the function of the cmeabc efflux pump were linked to the acquired fq resistance. these results ... | 2003 | 12499221 |
| antimicrobial susceptibilities of campylobacter strains isolated from finnish subjects infected domestically or from those infected abroad. | the in vitro susceptibilities of 678 campylobacter jejuni and campylobacter coli strains isolated from stool samples of the same number of finnish subjects were studied. a total of 523 patients, representing inhabitants from throughout finland, had not traveled abroad within the 2 weeks prior to becoming ill, whereas 155 persons had presumably acquired their infections abroad. the antimicrobial agents studied were erythromycin, ciprofloxacin, levofloxacin, trovafloxacin, and moxifloxacin. the mi ... | 2003 | 12499176 |
| increasing fluoroquinolone resistance in campylobacter jejuni, pennsylvania, usa,1982-2001. | fluoroquinolone-resistant campylobacter jejuni has been observed worldwide and is now being seen in the united states. among patients in our health-care system in pennsylvania, fluoroquinolone-resistant c. jejuni were not observed from 1982 to 1992; however, resistance increased to 40.5% in 2001. resistance to erythromycin remains at a low level (<5%). | 2002 | 12498672 |
| campylobacter jejuni cytolethal distending toxin promotes dna repair responses in normal human cells. | cytolethal distending toxin (cdt) is a multisubunit protein found in various gram-negative bacterial pathogens of humans which is thought to cause cell death by direct dna damage of host cells. we sought to determine if a cellular response to dna damage could be detected by exogenous addition of the holotoxin. exogenous addition of the campylobacter jejuni 81-176 cdt to primary human fibroblasts resulted in formation of rad50 foci, which are formed around double-stranded-dna breaks. moreover, su ... | 2003 | 12496208 |
| pathogenicity and immune response measured in mice following intranasal challenge with enterotoxigenic escherichia coli strains h10407 and b7a. | the pathogenicity and immunogenicity induced in balb/c mice by intranasal (i.n.) inoculation of enterotoxigenic escherichia coli (etec) strains h10407 (o78:h11:cfa/i:lt(+):st(+)) and b7a (o148:h28:cs6:lt(+):st(+)) (two etec strains previously used in human challenge trials) were studied. the i.n. inoculation of balb/c mice with large doses of etec strains h10407 and b7a caused illness and death. the h10407 strain was found to be consistently more virulent than the b7a strain. following i.n. chal ... | 2003 | 12496144 |
| campylobacter colonization of the crops of newly hatched leghorn chicks. | we have studied colonization of crops in newly hatched leghorn chicks (a layer breed) by wild-type and mutant strains of campylobacter jejuni. we established that the wild-type parent strain forms a stable population level within the crop and that the mutant strains will do likewise. concentrations of mutant strains in the crop were usually below that of the wild-type parent strain and ranged from 10(3) to 10(5) colony-forming units. these results differ from results we have previously reported ... | 2002 | 12495061 |
| fluoroquinolone resistance in campylobacter species from man and animals: detection of mutations in topoisomerase genes. | consecutive isolates of quinolone-resistant campylobacter isolated over a 5 year period (1990-1995) from the faeces of patients with enteritis in plymouth, uk, were examined for the epidemiology of mutations in gyra (n = 127). in addition, clinical isolates and poultry isolates from germany, the netherlands and other regions of the uk collected before 1995 were examined for mutations in the quinolone resistance-determining region of gyra by single-stranded conformational polymorphism analysis an ... | 2003 | 12493783 |
| surveillance of bacterial pathogens of diarrhea disease in indonesia. | emerging or reemerging infections due to bacterial disease may be a local, regional or global problem. bacterial acute gastroenteritis is a potential cause of substantial morbidity in travelers and deployed u.s. military personnel. a surveillance study was conducted over a two-year period in indonesia among 6760 patients with debilitating diarrheal diseases. of the 6,760 patients, 587 (9%) of the patient stools were positive for bacteria. the proportions of bacteria isolated from the 587 patient ... | 2002 | 12493168 |
| resistance to quinolones in campylobacter jejuni and campylobacter coli from danish broilers at farm level. | to investigate the prevalence of quinolone resistance among campylobacter jejuni and camp. coli isolates from danish poultry at the farm level, as well as for the whole country. | 2003 | 12492931 |
| comparative analysis of ribosomal proteins in complete genomes: an example of reductive evolution at the domain scale. | a comprehensive investigation of ribosomal genes in complete genomes from 66 different species allows us to address the distribution of r-proteins between and within the three primary domains. thirty-four r-protein families are represented in all domains but 33 families are specific to archaea and eucarya, providing evidence for specialisation at an early stage of evolution between the bacterial lineage and the lineage leading to archaea and eukaryotes. with only one specific r-protein, the arch ... | 2002 | 12490706 |
| interaction between probiotic lactic acid bacteria and canine enteric pathogens: a risk factor for intestinal enterococcus faecium colonization? | selected probiotic lactic acid bacteria (lab) have been shown to elicit positive health effects particularly in humans. competitive exclusion of pathogens is one of the most important beneficial health claims of probiotic bacteria. the effect of probiotic lab on competitive exclusion of pathogens has been demonstrated in humans, chicken and pigs. in this study we evaluated the ability of certain lab strains (lactobacillus rhamnosus gg, bifidobacterium lactis bb12, lactobacillus pentosus uk1a, l. ... | 2003 | 12488075 |
| a polyphosphate kinase (ppk2) widely conserved in bacteria. | synthesis of inorganic polyphosphate (poly p) from the terminal phosphate of atp is catalyzed reversibly by poly p kinase (ppk, now designated ppk1) initially isolated from escherichia coli. ppk1 is highly conserved in many bacteria, including some of the major pathogens such as pseudomonas aeruginosa. in a null mutant of p. aeruginosa lacking ppk1, we have discovered a previously uncharacterized ppk activity (designated ppk2) distinguished from ppk1 by the following: synthesis of poly p from gt ... | 2002 | 12486232 |
| common extracellular sensory domains in transmembrane receptors for diverse signal transduction pathways in bacteria and archaea. | transmembrane receptors in microorganisms, such as sensory histidine kinases and methyl-accepting chemotaxis proteins, are molecular devices for monitoring environmental changes. we report here that sensory domain sharing is widespread among different classes of transmembrane receptors. we have identified two novel conserved extracellular sensory domains, named chase2 and chase3, that are found in at least four classes of transmembrane receptors: histidine kinases, adenylate cyclases, predicted ... | 2003 | 12486065 |
| rapid pcr using nested primers of the 16s rrna and the hippuricase (hip o) genes to detect campylobacter jejuni and campylobacter coli in environmental samples. | identification of sources campylobacter infection in the poultry houses is in general problematic due to the lack of reliable methods to detect campylobacteria in environmental samples. detection of campylobacteria in environmental samples by conventional culture methods is difficult and of limited sensitivity due to the use of selective media, the low number of bacteria in the samples and possibly also due to the presence of non-culturable or sub-lethally injured stages of the bacteria. the pre ... | 2002 | 12477440 |
| elucidation of structure-function relationships in the protein subunit of bacterial rnase p using a genetic complementation approach. | rnase p is a ribonucleoprotein involved in trna biosynthesis in all living organisms. bacterial rnase p is comprised of a catalytic rna subunit and a lone protein cofactor which plays a supporting, albeit essential, role in the trna processing reaction in vivo. in this study, we have searched various databases to identify homologs of the protein subunit of rnase p from diverse bacteria and used an alignment of their primary sequences to determine the most highly conserved residues, and thereby e ... | 2002 | 12466529 |
| a strategy to retrieve the whole set of protein modules in microbial proteomes. | protein homology is often limited to long structural segments that we have previously called modules. we describe here a suite of programs used to catalog the whole set of modules present in microbial proteomes. first, the darwin allall program detects homologous segments using thresholds for evolutionary distance and alignment length, and another program classifies these modules. after assembling these homologous modules in families, we further group families which are related by a chain of nei ... | 2002 | 12466301 |
| bactericidal activity of phlorotannins from the brown alga ecklonia kurome. | the bactericidal activity of phlorotannins from brown algae against food-borne pathogenic bacteria (25 strains), methicillin-resistant staphylococcus aureus (mrsa) (nine strains) and streptococcus pyogenes (one strain) was examined and compared with that of catechins. in addition, the effect of the oral administration of phlorotannins on mice was investigated. phlorotannins, which are oligomers of phloroglucinol, were extracted from thalli of the brown alga ecklonia kurome and prepared by silici ... | 2002 | 12461009 |
| n-linked glycosylation in campylobacter jejuni and its functional transfer into e. coli. | n-linked protein glycosylation is the most abundant posttranslation modification of secretory proteins in eukaryotes. a wide range of functions are attributed to glycan structures covalently linked to asparagine residues within the asparagine-x-serine/threonine consensus sequence (asn-xaa-ser/thr). we found an n-linked glycosylation system in the bacterium campylobacter jejuni and demonstrate that a functional n-linked glycosylation pathway could be transferred into escherichia coli. although th ... | 2002 | 12459590 |
| [outbreak of gastroenteritis caused by campylobacter jejuni transmitted through drinking water]. | the aim of this study was to conduct a clinical-epidemiological and microbiological investigation into an outbreak of waterborne disease caused by campylobacter jejuni due to the consumption of drinking water. | 2002 | 12459108 |
| evolutionary analyses of the small subunit of glutamate synthase: gene order conservation, gene fusions, and prokaryote-to-eukaryote lateral gene transfers. | lateral gene transfer has been identified as an important mode of genome evolution within prokaryotes. except for the special case of gene transfer from organelle genomes to the eukaryotic nucleus, only a few cases of lateral gene transfer involving eukaryotes have been described. here we present phylogenetic and gene order analyses on the small subunit of glutamate synthase (encoded by gltd) and its homologues, including the large subunit of sulfide dehydrogenase (encoded by suda). the scattere ... | 2002 | 12455964 |
| colony multiplex pcr assay for identification and differentiation of campylobacter jejuni, c. coli, c. lari, c. upsaliensis, and c. fetus subsp. fetus. | a multiplex pcr assay was used to simultaneously detect genes from the five major clinically relevant campylobacter spp. those genes selected were hipo and 23s rrna from campylobacter jejuni; glya from each of c. coli, c. lari, and c. upsaliensis; and sapb2 from c. fetus subsp. fetus. the assay was evaluated with 137 clinical and environmental isolates and was found to be rapid and easy to perform and had a high sensitivity and specificity for characterizing isolates, even in mixed cultures. | 2002 | 12454184 |
| campylobacter jejuni in black-headed gulls (larus ridibundus): prevalence, genotypes, and influence on c. jejuni epidemiology. | campylobacteriosis is a zoonotic disease in which birds have been suggested to play an important role as a reservoir. we investigated the prevalence of campylobacter jejuni subsp. jejuni in black-headed gulls (larus ridibundus) in southern sweden with the aim of examining the nature of c. jejuni infection in this bird species. birds were sampled in four sampling series each year during 1999 (n = 419) and 2000 (n = 365). longitudinally sampled c. jejuni isolates from individual gulls were subject ... | 2002 | 12454158 |
| genetic diversity of intimin genes of attaching and effacing escherichia coli strains. | in this study, we determined the sequences of four intimin variant genes detected in attaching and effacing escherichia coli isolates of human origin. three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). the fourth was identical to the recently described eae-zeta (zeta), isolated from a bovine e. coli o84:nm isolate. we compared these sequences with those of published intimin-alpha, intimin-beta, intimin-gamma1, intimin-gamma2, intimin- epsilon, and ... | 2002 | 12454140 |
| direct identification in food samples of listeria spp. and listeria monocytogenes by molecular methods. | a new molecular approach for the detection and identification of listeria spp. and listeria monocytogenes in food is presented here. the method is based on the pcr amplification of a fragment of the iap gene from the five species belonging to the genus and on the analysis of the pcr products obtained by denaturing gradient gel electrophoresis (dgge). the protocol was first optimized by using strains from international collections. based on the differences present in the sequences amplified, it w ... | 2002 | 12450852 |
| molecular subtype analyses of campylobacter spp. from arkansas and california poultry operations. | campylobacter isolates from diverse samples within broiler production and processing environments were typed by using flaa short variable region dna sequence analysis. sixteen flocks from four different farms representing two broiler producers in arkansas and california were analyzed. fourteen of the flocks (87.5%) were campylobacter-positive; two remained negative throughout the 6-week rearing period. in general, multiple clones were present within a flock. additionally, clones found within a f ... | 2002 | 12450847 |
| prevalence of campylobacter jejuni, campylobacter lari, and campylobacter coli in different ecological guilds and taxa of migrating birds. | a total of 1,794 migrating birds trapped at a coastal site in southern sweden were sampled for detection of campylobacter spp. all isolates phenotypically identified as campylobacter jejuni and a subset of those identified as non-c. jejuni were identified to the species level by pcr-based techniques. c. jejuni was found in 5.0% of the birds, campylobacter lari was found in 5.6%, and campylobacter coli was found in 0.9%. an additional 10.7% of the tested birds were infected with hippurate hydroly ... | 2002 | 12450810 |
| bacterial chemotaxis toward environmental pollutants: role in bioremediation. | 2002 | 12450797 | |
| fibronectin-facilitated invasion of t84 eukaryotic cells by campylobacter jejuni occurs preferentially at the basolateral cell surface. | previous studies have indicated that the ability to bind to fibronectin is a key feature in successful cell invasion by campylobacter jejuni. given the spatial distribution of fibronectin and the architecture of the epithelium, this suggests the possibility that c. jejuni cell invasion might preferentially occur at the basolateral cell surface. to test this hypothesis, we examined the interaction of c. jejuni with t84 human colonic cells. when grown under the appropriate conditions, t84 cells fo ... | 2002 | 12438340 |
| speciating campylobacter jejuni and campylobacter coli isolates from poultry and humans using six pcr-based assays. | six previously published polymerase chain reaction (pcr) assays each targeting different genes were used to speciate 116 isolates previously identified as campylobacter jejuni using routine microbiological techniques. of the 116 isolates, 84 were of poultry origin and 32 of human origin. the six pcr assays confirmed the species identities of 31 of 32 (97%) human isolates and 56 of 84 (67%) poultry isolates as c. jejuni. twenty eight of 84 (33%) poultry isolates were identified as campylobacter c ... | 2002 | 12435503 |
| structures of two polysaccharides of campylobacter jejuni 81116. | campylobacter jejuni 81116 has been extensively investigated in studies on genes associated with the synthesis of campylobacter lipopoly/lipooligosaccharides (lps/los). despite these investigations, data on the chemical structure of polysaccharides from c. jejuni 81116 have been absent. the present study was undertaken to fill that void. biomass was grown in large quantities on agar medium, harvested and extracted by hot phenol-water extraction. subsequently, extracts were treated by dnase, rnas ... | 2002 | 12433486 |
| identification of campylobacter jejuni isolates from cloacal and carcass swabs of chickens in thailand by a 5' nuclease fluorogenic polymerase chain reaction assay. | a rapid 5' nuclease fluorogenic polymerase chain reaction (pcr) assay for identifying campylobacter jejuni was applied to campylobacter isolates from chicken cloacal and carcass swabs collected from three chicken farms and a slaughterhouse in thailand. the primers and the probe were based on the sequence of the gyra gene in c jejuni. c. jejuni isolates were identified by fluorogenic pcr assay of bacterial cells directly from campylobacter-selective agar medium. this assay allowed the identificat ... | 2002 | 12430691 |
| peripheral neuropathies and anti-glycolipid antibodies. | this review charts the progress of anti-glycolipid antibodies in neuropathy, from their original discovery 20 years ago in immunoglobulin m paraproteinaemic neuropathy through to current discoveries mapping their relationship to subtypes of guillain-barré syndrome. antibodies to >20 different glycolipids have now been associated with a wide range of clinically identifiable acute and chronic neuropathy syndromes. particular progress has been achieved in understanding the link between acute motor ... | 2002 | 12429589 |
| improved analytical methods for microarray-based genome-composition analysis. | whereas genome sequencing has given us high-resolution pictures of many different species of bacteria, microarrays provide a means of obtaining information on genome composition for many strains of a given species. genome-composition analysis using microarrays, or 'genomotyping', can be used to categorize genes into 'present' and 'divergent' categories based on the level of hybridization signal. this typically involves selecting a signal value that is used as a cutoff to discriminate present (hi ... | 2002 | 12429064 |
| the prc-barrel: a widespread, conserved domain shared by photosynthetic reaction center subunits and proteins of rna metabolism. | the h subunit of the purple bacterial photosynthetic reaction center (prc-h) is important for the assembly of the photosynthetic reaction center and appears to regulate electron transfer during the reduction of the secondary quinone. it contains a distinct cytoplasmic beta-barrel domain whose fold has no close structural relationship to any other well known beta-barrel domain. | 2002 | 12429060 |
| role of nerves in enteric infection. | peripheral and central effects of enteric infection are considered. nerves play a vital part in the immediate response to enteric infection, promoting pathogen expulsion by orchestrating intestinal secretion and propulsive motor patterns. laboratory studies indicate that therapeutic agents aimed at modulating the neural response can profoundly alter the outcome of infection. as our understanding of the role of nerves increases, exciting new targets for therapeutic intervention will emerge in bot ... | 2002 | 12427768 |
| occurrence of yersinia enterocolitica and campylobacter spp. in slaughter pigs and consequences for meat inspection, slaughtering, and dressing procedures. | the purpose of the present investigation was to assess the occurrence of yersinia enterocolitica and campylobacter spp. in the lymphoid tissues and intestinal tract in pigs and the risk for contamination during the compulsory meat inspection procedures and the procedures during slaughtering and dressing. another objective of the investigation was to compare traditional isolation methods, the use of a polymerase chain reaction (pcr) method (bugs'n beads bacterial dna isolation kit) and an elisa m ... | 2003 | 12423925 |
| genetic evidence for a novel thymidylate synthase in the halophilic archaeon halobacterium salinarum and in campylobacter jejuni. | a search of the complete genome sequence of the halophilic archaeon halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thya) gene present in the closely related haloferax volcanii. to understand the source of thymidine synthesis in hbt. salinarum, a genomic library of hbt. salinarum was constructed and used to complement a hfx. volcanii thya deletion mutation. the hbt. salinarum orf that complemented the thya mutation shares sequence homology with orfs foun ... | 2002 | 12423760 |
| prediction, assessment and validation of protein interaction maps in bacteria. | high-throughput proteomics technologies, especially the yeast two-hybrid system, produce large volumes of protein-protein interaction data organized in networks. the complete sequencing of many genomes raises questions about the extent to which such networks can be transferred between organisms. we attempted to answer this question using the experimentally derived helicobacter pylori interaction map and the recently described interacting domain profile pair (idpp) method to predict a virtual map ... | 2002 | 12419263 |
| fulminant guillain-barré syndrome after campylobacter jejuni enteritis and monospecific anti-gt1a igg antibody. | a 21-year-old man developed rapid progression of tetraplegia, bulbar palsy, and respiratory paralysis after campylobacterjejuni enteritis. based on the diagnosis of guillain-barré syndrome, he received plasmapheresis and intravenous immunoglobulin. serum anti-gt1a igg antibody which lacked cross-reactivity with gq1b was detected. four months after the onset, the patient still had severe muscle weakness of the lower limbs. this case suggests that anti-gt1a igg antibody can be associated with seve ... | 2002 | 12413017 |
| fulminant guillain-barrè syndrome after campylobacter jejuni enteritis and anti-ganglioside antibody. | 2002 | 12412991 | |
| the phage n4 virion rna polymerase catalytic domain is related to single-subunit rna polymerases. | in vitro, bacteriophage n4 virion rna polymerase (vrnap) recognizes in vivo sites of transcription initiation on single-stranded templates. n4 vrnap promoters are comprised of a hairpin structure and conserved sequences. here, we show that vrnap consists of a single 3500 amino acid polypeptide, and we define and characterize a transcriptionally active 1106 amino acid domain (mini-vrnap). biochemical and genetic characterization of this domain indicates that, despite its peculiar promoter specifi ... | 2002 | 12411499 |
| wound botulism associated with subcutaneous drug use. | 2002 | 12411365 | |
| rate of occurrence and pathogenic effect of enteroaggregative escherichia coli virulence factors in international travelers. | one or more putative enteroaggregative escherichia coli (eaec) virulence factors (agga, aggr, aspu, or aafa) were identified in 60 (70%) of 86 eaec isolates from travelers with diarrhea compared with a rate of 7 (8%) of 90 in patients with diarrhea who were infected with nonadherent e. coli (odds ratio, 27.36; 95% confidence interval, 11.30 to 65.91). the presence of aggr or one or more virulence factors in eaec from patients with diarrhea was associated with a statistically increased concentrat ... | 2002 | 12409395 |
| detection of gyra mutations in quinolone-resistant salmonella enterica by denaturing high-performance liquid chromatography. | denaturing high-performance liquid chromatography (dhplc) was evaluated as a rapid screening and identification method for dna sequence variation detection in the quinolone resistance-determining region of gyra from salmonella serovars. a total of 203 isolates of salmonella were screened using this method. dhplc analysis of 14 isolates representing each type of novel or multiple mutations and the wild type were compared with lightcycler-based pcr-gyra hybridization mutation assay (gama) and sing ... | 2002 | 12409384 |