Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| evaluation of the temporal association between kawasaki disease and viral infections in south korea. | this study is aimed at elucidating potential temporal associations between the occurrence of kawasaki disease (kd) and various viral infections. | 2014 | 25089137 |
| antiviral activity of baicalin against influenza a (h1n1/h3n2) virus in cell culture and in mice and its inhibition of neuraminidase. | scutellaria baicalensis georgi, a chinese herbal decoction, has been used for the treatment of the common cold, fever and influenza virus infections. in previous studies, we found that oral administration of baicalein resulted in the inhibition of influenza a virus replication in vivo, which was linked to baicalin in serum. however, the effective dose and underlying mechanisms of the efficacy of baicalin against influenza a virus have not been fully elucidated. in this study, the antiviral effec ... | 2014 | 25078390 |
| [evaluation of the sensitivity of a densitometry system, in judging the result of influenza virus antigen-detection kit using immunochromatography]. | immunochromatography viral antigen-detection kits have become popular in clinical settings in japan. influenza virus detection kit is one of them. it is sometimes used in early phase of the disease, combined with the early treatment with anti-influenza drugs. most of them are invented to visually read the test line on their kits. however, we should be careful about their reliability of them. sometimes human errors occur at the visual tests, and they have different sensitivities among the kits fr ... | 2014 | 25073199 |
| detection of h3n2 canine influenza virus using a quartz crystal microbalance. | label-free technology-based quartz crystal microbalance (qcm) is an emerging tool in biological research. in this study, qcm was applied successfully for the rapid diagnosis of h3n2 canine influenza virus (civ) infection. prolinker™ b, a calixcrown derivative, enables antibodies to be attached to a gold-coated quartz surface and positioned in a regular pattern with the correct orientation. the prolinker-coated quartz-based assay detected h3n2 civ at lower concentrations (2(2) ha unit) than a com ... | 2014 | 25072379 |
| [the peculiarities of the influenza epidemics in some areas of russia during 2012-2013 season. the influenza a (h1n1) pdm09 virus domination in european countries]. | the peculiarities of the influenza viruses circulation in 2012-2013 are discussed. the results were obtained in 10 cities of russia, where basic laboratories of the influenza ecology and epidemics center of on the basis of ivanovsky institute of virology, ministry of health of the russian federation, are situated. the increasing rate of the ard morbidity caused by influenza viruses was observed in january-march 2013. the highest indices of the morbidity were detected during 6-7 weeks with the fo ... | 2016 | 25069278 |
| an h1-h3 chimeric influenza virosome confers complete protection against lethal challenge with pr8 (h1n1) and x47 (h3n2) viruses in mice. | annual health threats and economic damages caused by influenza virus are still a main concern of the world health organization and other health departments all over the world. an influenza virosome is a highly efficient immunomodulating carrier mimicking the natural antigen presentation pathway and has shown an excellent tolerability profile due to its biocompatibility and purity. the major purpose of this study was to construct a new chimeric virosome influenza vaccine containing hemagglutinin ... | 2014 | 25066138 |
| chimeric influenza-virus-like particles containing the porcine reproductive and respiratory syndrome virus gp5 protein and the influenza virus ha and m1 proteins. | both porcine reproductive and respiratory syndrome and swine influenza are acute, highly contagious swine diseases. these diseases pose severe threats for the swine industry and cause heavy economic losses worldwide. in this study, we have developed a chimeric virus-like particle (vlp) vaccine candidate for porcine reproductive and respiratory syndrome virus (prrsv) and h3n2 influenza virus and investigated its immunogenicity in mice. the ha and m1 proteins from the h3n2 influenza virus and the ... | 2014 | 25064513 |
| genomic reassortants of pandemic a (h1n1) 2009 virus and endemic porcine h1 and h3 viruses in swine in japan. | from 2010 to 2013 in japan, we isolated 11 swine influenza viruses (sivs) from pigs showing respiratory symptoms. sequence and phylogenetic analyses showed that 6 h1n1 viruses originated from the pandemic (h1n1) 2009 (pdm 09) virus and the other 5 viruses were reassortants between sivs and pdm 09 viruses, representing 4 genotypes. two h1n2 viruses contained h1 and n2 genes originated from japanese h1n2 siv together with internal genes of pdm 09 viruses. additionally, 1 h1n2 virus contained a fur ... | 2014 | 25056678 |
| neuraminidase gene homology contributes to the protective activity of influenza vaccines prepared from the influenza virus library. | whole-virus (wv) vaccines from influenza a/duck/hokkaido/77 (h3n2), and its reassortant strains h3n4, h3n5 and h3n7, which have the same haemagglutinin (ha) gene but different neuraminidase (na) genes, were prepared from our influenza virus library. mice were intranasally immunized with equivalent doses of each vaccine (1-0.01 µg per mouse). all of the mice that received the highest dose of each vaccine (1 µg per mouse) showed equivalent high ha-inhibiting (hi) antibody titres and survived the h ... | 2014 | 25053564 |
| targeting the ha2 subunit of influenza a virus hemagglutinin via cd40l provides universal protection against diverse subtypes. | the influenza viral hemagglutinin (ha) is comprised of two subunits. current influenza vaccine predominantly induces neutralizing antibodies (abs) against the ha1 subunit, which is constantly evolving in unpredictable fashion. the other subunit, ha2, however, is highly conserved but largely shielded by the ha head domain. thus, enhancing immune response against ha2 could potentially elicit broadly inhibitory abs. we generated a recombinant adenovirus (rad) encoding secreted fusion protein, consi ... | 2015 | 25052763 |
| azapropellanes with anti-influenza a virus activity. | the synthesis of several [4,4,3], [4,3,3], and [3,3,3]azapropellanes is reported. several of the novel amines displayed low-micromolar activities against an amantadine-resistant h1n1 strain, but they did not show activity against an amantadine-sensitive h3n2 strain. none of the tested compounds inhibit the influenza a/m2 proton channel function. most of the compounds did not show cytotoxicity for mdck cells. | 2014 | 25050174 |
| global update on the susceptibility of human influenza viruses to neuraminidase inhibitors, 2012-2013. | emergence of influenza viruses with reduced susceptibility to neuraminidase inhibitors (nais) is sporadic, often follows exposure to nais, but occasionally occurs in the absence of nai pressure. the emergence and global spread in 2007/2008 of a(h1n1) influenza viruses showing clinical resistance to oseltamivir due to neuraminidase (na) h275y substitution, in the absence of drug pressure, warrants continued vigilance and monitoring for similar viruses. four world health organization (who) collabo ... | 2014 | 25043638 |
| divergent immune responses and disease outcomes in piglets immunized with inactivated and attenuated h3n2 swine influenza vaccines in the presence of maternally-derived antibodies. | live-attenuated influenza virus (laiv) prime-boost vaccination previously conferred protection against heterologous h3n2 swine influenza challenge, including in piglets with maternally derived antibodies (mda). conversely, a whole-inactivated virus (wiv) vaccine was associated with enhanced disease. this study was aimed at identifying immune correlates of cross-protection. piglets with and without mda received intramuscular adjuvanted wiv or intranasal laiv, and were challenged with heterologous ... | 2014 | 25043588 |
| use of fractional factorial design to study the compatibility of viral ribonucleoprotein gene segments of human h7n9 virus and circulating human influenza subtypes. | avian h7n9 influenza viruses may pose a further threat to humans by reassortment with human viruses, which could lead to generation of novel reassortants with enhanced polymerase activity. we previously established a novel statistical approach to study the polymerase activity of reassorted vrnps (influenza other respir viruses. 2013;7:969-78). here, we report the use of this method to study recombinant vrnps with subunits derived from human h1n1, h3n2, and h7n9 viruses. our results demonstrate t ... | 2014 | 25043276 |
| effectiveness of trivalent seasonal influenza vaccine in preventing laboratory-confirmed influenza in primary care in the united kingdom: 2012/13 end of season results. | the effectiveness of the 2012/13 trivalent seasonal influenza vaccine (tiv) was assessed using a test-negative case-control study of patients consulting primary care with influenza-like illness in the united kingdom. strain characterisation was undertaken on selected isolates. vaccine effectiveness (ve) against confirmed influenza a(h3n2), a(h1n1) and b virus infection, adjusted for age, sex, surveillance scheme (i.e. setting) and month of sample collection was 26% (95% confidence interval (ci): ... | 2014 | 25033051 |
| influenza and other respiratory viruses involved in severe acute respiratory disease in northern italy during the pandemic and postpandemic period (2009-2011). | since 2009 pandemic, international health authorities recommended monitoring severe and complicated cases of respiratory disease, that is, severe acute respiratory infection (sari) and acute respiratory distress syndrome (ards). we evaluated the proportion of sari/ards cases and deaths due to influenza a(h1n1)pdm09 infection and the impact of other respiratory viruses during pandemic and postpandemic period (2009-2011) in northern italy; additionally we searched for unknown viruses in those case ... | 2014 | 25013770 |
| [an international collaborative study on influenza viruses antibody titers and contact patterns of individuals in rural and urban household of guangzhou]. | to describe the influenza viruses antibody levels and contact patterns of individuals in rural and urban regions of guangzhou and to understand how contact patterns and other factors would correlate with the levels on the titers of antibody. | 2014 | 25009036 |
| expansion of genotypic diversity and establishment of 2009 h1n1 pandemic-origin internal genes in pigs in china. | two-way transmission of influenza viruses between humans and swine has been frequently observed, and the occurrence of the 2009 h1n1 pandemic influenza virus (pdm/09) demonstrated that swine-origin viruses could facilitate the genesis of a pandemic strain. although multiple introductions to and reassortment in swine of the pdm/09 virus have been repeatedly reported in both eurasia and the americas, its long-term impact on the development of swine influenza viruses (sivs) has not been systematica ... | 2014 | 25008935 |
| glycan based detection and drug susceptibility of influenza virus. | we have developed a panel of synthetic glycans as receptor mimics for the specific capture of influenza viruses. the glycans were printed onto commercial glass slides using a free amine at the end of a spacer to generate a small focused microarray. the microarray was evaluated for its ability to capture three different strains of influenza a virus, two h1n1, a/brisbane/59/2007 and a/solomon islands/3/2006 and one h3n2, a/aichi/2/1968. we observed an excellent detection ability with some compound ... | 2014 | 25007351 |
| viral etiology and seasonality of influenza-like illness in gabon, march 2010 to june 2011. | surveillance of influenza-like illness (ili) in central africa began only recently, and few data are therefore available on the circulation of influenza virus and other respiratory viruses. in gabon, a central african country, we established a surveillance network in four major towns in order to analyze cases of ili among patients who visited health centers between march 2010 and june 2011, and to determine the viral etiology. | 2014 | 25000832 |
| intranasal chitosan-dna vaccines that protect across influenza virus subtypes. | an egg-free and broadly effective influenza vaccine that can be produced rapidly, adequately and cost-effectively is needed. in this study, chitosan-associated dnas prepared at various nitrogen/phosphate charge (n/p) ratios were studied for their physicochemical properties, stability, cytotoxicity, and protein expression ability. the chitosan-dna complexes (chitoplexes) of the n/p ratio 2 had the required characteristics including optimal size range, positive surface charge, high dna association ... | 2014 | 24998507 |
| simflu: a simulation tool for predicting the variation pattern of influenza a virus. | since the first pandemic outbreak of avian influenza a virus (h5n1 subtype) in 1997, the national center for biotechnology information (ncbi) has provided a large number of influenza virus sequences with well-organized annotations. using the time-series sequences of influenza a viruses, we developed a simulation tool for influenza virus, named simflu, to predict possible future variants of influenza viruses. simflu can create variants from a seed nucleotide sequence of influenza a virus using th ... | 2014 | 24995426 |
| recent h3n2 influenza virus clinical isolates rapidly acquire hemagglutinin or neuraminidase mutations when propagated for antigenic analyses. | prior to serological testing, influenza viruses are typically propagated in eggs or cell culture. recent human h3n2 strains bind to cells with low avidity. here, we isolated nine primary h3n2 viral isolates from respiratory secretions of children. upon propagation in vitro, five of these isolates acquired hemagglutinin or neuraminidase mutations that increased virus binding to cell surfaces. these mutations can potentially confound serological assays commonly used to identify antigenically novel ... | 2014 | 24991002 |
| evaluation of alere i influenza a&b for rapid detection of influenza viruses a and b. | rapid and accurate diagnosis of influenza is important for infection control, as well as for patient management. alere i influenza a&b is an isothermal nucleic acid amplification-based integrated system for detection and differentiation of influenza virus a and influenza virus b. the performance of the alere i influenza a&b was screened using frozen nasopharyngeal-swab specimens collected in viral transport medium (vtm) that were originally tested fresh with the filmarray respiratory panel (rp) ... | 2014 | 24989611 |
| alzheimer's associated β-amyloid protein inhibits influenza a virus and modulates viral interactions with phagocytes. | accumulation of β-amyloid (βa) is a key pathogenetic factor in alzheimer's disease; however, the normal function of βa is unknown. recent studies have shown that βa can inhibit growth of bacteria and fungi. in this paper we show that βa also inhibits replication of seasonal and pandemic strains of h3n2 and h1n1 influenza a virus (iav) in vitro. the 42 amino acid fragment of βa (βa42) had greater activity than the 40 amino acid fragment. direct incubation of the virus with βa42 was needed to achi ... | 2014 | 24988208 |
| h3n2 canine influenza virus with the matrix gene from the pandemic a/h1n1 virus: infection dynamics in dogs and ferrets. | after an outbreak of pandemic influenza a/h1n1 (ph1n1) virus, we had previously reported the emergence of a recombinant canine influenza virus (civ) between the ph1n1 virus and the classic h3n2 civ. our ongoing routine surveillance isolated another reassortant h3n2 civ carrying the matrix gene of the ph1n1 virus from 2012. the infection dynamics of this h3n2 civ variant (civ/h3n2mv) were investigated in dogs and ferrets via experimental infection and transmission. the civ/h3n2mv-infected dogs an ... | 2015 | 24977303 |
| performance characteristics of qualified cell lines for isolation and propagation of influenza viruses for vaccine manufacturing. | cell culture is now available as a method for the production of influenza vaccines in addition to eggs. in accordance with currently accepted practice, viruses recommended as candidates for vaccine manufacture are isolated and propagated exclusively in hens' eggs prior to distribution to manufacturers. candidate vaccine viruses isolated in cell culture are not available to support vaccine manufacturing in mammalian cell bioreactors so egg-derived viruses have to be used. recently influenza a (h3 ... | 2014 | 24975811 |
| estimates of mortality attributable to influenza and rsv in the united states during 1997-2009 by influenza type or subtype, age, cause of death, and risk status. | influenza and respiratory syncytial virus (rsv) cause substantial mortality from respiratory and other causes in the usa, especially among people aged 65 and older. | 2014 | 24975705 |
| universal immunity to influenza must outwit immune evasion. | although an influenza vaccine has been available for 70 years, influenza virus still causes seasonal epidemics and worldwide pandemics. currently available vaccines elicit strain-specific antibody (ab) responses to the surface haemagglutinin (ha) and neuraminidase (na) proteins, but these can be ineffective against serologically-distinct viral variants and novel subtypes. thus, there is a great need for cross-protective or "universal" influenza vaccines to overcome the necessity for annual immun ... | 2014 | 24971078 |
| surveillance of influenza virus during 2010-2012 in changsha, china. | the objective of this study was to examine the circulating influenza viruses in changsha, china, during 2010-2012. nasopharyngeal specimens were collected from persons with influenza-like illness (ili) who presented for care at two hospitals. of 2,955 patients tested, 278/(9.4%) were positive for influenza virus: 116/(41.7%) were influenza type a(h3n2), 79/(28.4%) were type a(h1n1) pandemic 2009 (pdm09) and 83/(29.9%) were influenza type b. the rates of virus detection varied by age and sex. the ... | 2014 | 24968671 |
| influenza vaccination accelerates recovery of ferrets from lymphopenia. | ferrets are a useful animal model for human influenza virus infections, since they closely mimic the pathogenesis of influenza viruses observed in humans. however, a lack of reagents, especially for flow cytometry of immune cell subsets, has limited research in this model. here we use a panel of primarily species cross-reactive antibodies to identify ferret t cells, cytotoxic t lymphocytes (ctl), b cells, and granulocytes in peripheral blood. following infection with seasonal h3n2 or h1n1pdm09 i ... | 2014 | 24968319 |
| higher vaccine effectiveness in seasons with predominant circulation of seasonal influenza a(h1n1) than in a(h3n2) seasons: test-negative case-control studies using surveillance data, spain, 2003-2011. | we used data provided by the spanish influenza surveillance system to measure seasonal influenza vaccine effectiveness (ve) against medically attended cases, laboratory confirmed with the predominately circulating influenza virus over eight seasons (2003-2011). | 2014 | 24968153 |
| introductions and evolution of human-origin seasonal influenza a viruses in multinational swine populations. | the capacity of influenza a viruses to cross species barriers presents a continual threat to human and animal health. knowledge of the human-swine interface is particularly important for understanding how viruses with pandemic potential evolve in swine hosts. we sequenced the genomes of 141 influenza viruses collected from north american swine during 2002 to 2011 and identified a swine virus that possessed all eight genome segments of human seasonal a/h3n2 virus origin. a molecular clock analysi ... | 2014 | 24965467 |
| serological report of pandemic and seasonal human influenza virus infection in dogs in southern china. | from january to july 2012, we looked for evidence of subclinical a (h1n1) pdm09 and seasonal human influenza viruses infections in healthy dogs in china. sera from a total of 1920 dogs were collected from guangdong, guangxi, fujian and jiangxi provinces. we also examined archived sera from 66 dogs and cats that were collected during 2008 from these provinces. using hemagglutination inhibition (hi) and microneutralization (mn) assays, we found that only the dogs sampled in 2012 had elevated antib ... | 2014 | 24965232 |
| epidemiology of influenza virus types and subtypes in south africa, 2009-2012. | to determine clinical and epidemiologic differences between influenza caused by different virus types and subtypes, we identified patients and tested specimens. patients were children and adults hospitalized with confirmed influenza and severe acute respiratory illness (sari) identified through active, prospective, hospital-based surveillance from 2009-2012 in south africa. respiratory specimens were tested, typed, and subtyped for influenza virus by pcr. of 16,005 sari patients tested, 1,239 (8 ... | 2014 | 24960314 |
| characterization of drug-resistant influenza virus a(h1n1) and a(h3n2) variants selected in vitro with laninamivir. | neuraminidase inhibitors (nais) play a major role for managing influenza virus infections. the widespread oseltamivir resistance among 2007-2008 seasonal a(h1n1) viruses and community outbreaks of oseltamivir-resistant a(h1n1)pdm09 strains highlights the need for additional anti-influenza virus agents. laninamivir is a novel long-lasting nai that has demonstrated in vitro activity against influenza a and b viruses, and its prodrug (laninamivir octanoate) is in phase ii clinical trials in the uni ... | 2014 | 24957832 |
| conservation of t cell epitopes between seasonal influenza viruses and the novel influenza a h7n9 virus. | a novel avian influenza a (h7n9) virus recently emerged in the yangtze river delta and caused diseases, often severe, in over 130 people. this h7n9 virus appeared to infect humans with greater ease than previous avian influenza virus subtypes such as h5n1 and h9n2. while there are other potential explanations for this large number of human infections with an avian influenza virus, we investigated whether a lack of conserved t-cell epitopes between endemic h1n1 and h3n2 influenza viruses and the ... | 2014 | 24950786 |
| significant cross reactive antibodies to influenza virus in adults and children during a period of marked antigenic drift. | little is known about the development of cross-reactive antibodies following natural exposure to pathogens. such knowledge is critical in the development of new universal influenza vaccines. | 2014 | 24950742 |
| a packaged paper fluidic-based microdevice for detecting gene expression of influenza a virus. | pathotyping and subtyping of influenza a virus were performed with a packaged paper fluidic-based analytical microdevice (pfam) after one-step reverse transcription-polymerase chain reaction (rt-pcr). the pfam contains two test lines: one for detecting m gene to identify the influenza a virus and another for haemagglutinin subtyping to determine the viral strain among h1n1, h3n2, and h5n1. the m gene and the haemagglutinin gene (h1, h3, and h5 genes) were amplified by using the digoxigenin and t ... | 2014 | 24949821 |
| identification and genetic characterization of avian-origin h3n2 canine influenza viruses isolated from the liaoning province of china in 2012. | a total of 158 serum samples and 510 nasal swab specimens were collected between september 2010 and may 2012, from dogs exhibiting respiratory symptoms, in order to investigate the epidemiology of h3n2 canine influenza viruses (civs) in the liaoning province of china. serological surveillance demonstrated that 10.8 % (17/158) of serum samples were positive for h3n2 canine influenza. two h3n2 influenza viruses, a/canine/liaoning/27/2012 and a/canine/liaoning/h6/2012, were isolated from pet dogs i ... | 2014 | 24928168 |
| serological and molecular prevalence of swine influenza virus on farms in northwestern mexico. | the aim of this study was to provide an overview of the epidemiological status of swine influenza viruses in pigs from northwestern mexico in 2008-2009. a serological and molecular survey was conducted in 150 pigs from 15 commercial farms in sonora, mexico (northwestern region of mexico). the serological data showed that 55% of the sera were positive for the h1n1 subtype, 59% for the h3n2 subtype, and 38% for both subtypes. overall, 16.6% (25/150) of the samples were positive for type a influenz ... | 2014 | 24925324 |
| molecular epidemiology and evolution of a(h1n1)pdm09 and h3n2 virus during winter 2012-2013 in beijing, china. | in order to evaluate the epidemiology of influenza a and its surface antigens (haemagglutinin [ha] and neuraminidase [na]) for molecular epidemiology and evolution analysis during winter 2012-2013 in beijing, china, we worked within the framework of the chinese national influenza center and collected nasal swabs of patients presenting with influenza-like illness. we found that both a(h1n1)pdm09 (46.8%) and h3n2 (53.2%) viruses were the predominant strains during the 2012-2013 influenza epidemic. ... | 2014 | 24911284 |
| design, synthesis, antiviral and cytostatic activity of ω-(1h-1,2,3-triazol-1-yl)(polyhydroxy)alkylphosphonates as acyclic nucleotide analogues. | the efficient synthesis of a new series of polyhydroxylated dibenzyl ω-(1h-1,2,3-triazol-1-yl)alkylphosphonates as acyclic nucleotide analogues is described starting from dibenzyl ω-azido(polyhydroxy)alkylphosphonates and selected alkynes under microwave irradiation. selected o,o-dibenzylphosphonate acyclonucleotides were transformed into the respective phosphonic acids. all compounds were evaluated in vitro for activity against a broad variety of dna and rna viruses and for cytostatic activity ... | 2014 | 24906510 |
| a brief introduction to influenza a virus in swine. | influenza a viruses (iav) of the orthomyxoviridae virus family cause one of the most important respiratory diseases in pigs as well as humans. repeated outbreaks and rapid spread of genetically and antigenically distinct iavs represent a considerable challenge for animal production and public health. this overlap between human and animal health is a prime example of the "one health" concept. although only subtypes of h1n1, h1n2, and h3n2 are endemic in swine around the world, considerable divers ... | 2014 | 24899434 |
| influenza virus h1n1 inhibition by serine protease inhibitor (serpin) antithrombin iii. | endogenous serine protease inhibitors (serpins) are anti-inflammatory mediators with multiple biologic functions. serpins are also part of the early innate immune response to viral infection that includes mannose binding lectins, soluble cd14, defensins and antimicrobial peptides. recently, serpin antithrombin iii (atiii) was shown to have broad-spectrum antiviral activity against hiv, hsv and hcv. we tested atiii's antiviral activity against a variety of influenza virus strains. in our studies ... | 2014 | 24883334 |
| detection of avian influenza a/h7n9/2013 virus by real-time reverse transcription-polymerase chain reaction. | the first case of avian influenza a/h7n9 infection was reported in shanghai in mid-february, 2013; by may 1, 2013, it had infected 127 people and caused 26 deaths in 10 provinces in china. therefore, it is important to obtain reliable epidemiological data on the spread of this new infectious agent, a need that may be best met by the development of novel molecular methods. here, a new method was described for the detection of avian influenza a/h7n9 using real-time reverse transcription-polymerase ... | 2014 | 24880072 |
| isolation and identification of phase i metabolites of phillyrin in rats. | phillyrin was one of the main chemical constituents of the fruit of forsythia suspensa (thunb.) vahl. it showed various bioactivities including antioxidant and anti-inflammatory activities. however, the metabolism of phillyrin remained unknown. this report described the isolation and identification of phase i metabolites of phillyrin in rats. nine metabolites including six new ones were isolated by various column chromatographies and high-performance liquid chromatography. their structures were ... | 2014 | 24879901 |
| [monitoring of activity of influenza in santa fe, argentina, 2005-2010]. | influenza viruses have a relevant public health impact. | 2014 | 24878901 |
| activation of c-jun n-terminal kinase upon influenza a virus (iav) infection is independent of pathogen-related receptors but dependent on amino acid sequence variations of iav ns1. | a hallmark cell response to influenza a virus (iav) infections is the phosphorylation and activation of c-jun n-terminal kinase (jnk). however, so far it is not fully clear which molecules are involved in the activation of jnk upon iav infection. here, we report that the transfection of influenza viral-rna induces jnk in a retinoic acid-inducible gene i (rig-i)-dependent manner. however, neither rig-i-like receptors nor myd88-dependent toll-like receptors were found to be involved in the activat ... | 2014 | 24872593 |
| influenza virus pb1 and neuraminidase gene segments can cosegregate during vaccine reassortment driven by interactions in the pb1 coding region. | egg-grown influenza vaccine yields are maximized by infection with a seed virus produced by "classical reassortment" of a seasonal isolate with a highly egg-adapted strain. seed viruses are selected based on a high-growth phenotype and the presence of the seasonal hemagglutinin (ha) and neuraminidase (na) surface antigens. retrospective analysis of h3n2 vaccine seed viruses indicated that, unlike other internal proteins that were predominantly derived from the high-growth parent a/puerto rico/8/ ... | 2014 | 24872588 |
| influenza a virus and tlr7 activation potentiate nox2 oxidase-dependent ros production in macrophages. | influenza a virus infects resident alveolar macrophages in the respiratory tract resulting in toll like receptor 7 (tlr7) activation that triggers an inflammatory response to resolve the infection. macrophages are also major sources of reactive oxygen species (ros) via the nox2-containing nadph oxidase. although ros are crucial for pathogen clearance, in response to influenza a virus, ros are touted as being culprit mediators of the lung tissue injury. the aim of the present study was to determi ... | 2014 | 24869957 |
| a prospective study of romanian agriculture workers for zoonotic influenza infections. | in this prospective study we sought to examine seroepidemiological evidence for acute zoonotic influenza virus infection among romanian agricultural workers. | 2014 | 24869796 |
| [optimization of culture condition for cultivation of influenza virus a on mdck cell]. | to optimize the cultivation condition of influenza virus a on mdck cells and to improve the separation rate and the inspected effect of influenza virus. | 2014 | 24868970 |
| safety and immunogenicity in man of a cell culture derived trivalent live attenuated seasonal influenza vaccine: a phase i dose escalating study in healthy volunteers. | live attenuated influenza vaccine (laiv) offers the promise of inducing a variety of immune responses thereby conferring protection to circulating field strains. laivs are based on cold adapted and temperature sensitive phenotypes of master donor viruses (mdvs) containing the surface glycoprotein genes of seasonal influenza strains. two types of mdv lineages have been described, the ann arbor lineages and the a/leningrad/17 and b/ussr/60 lineages. here the safety and immunogenicity of a madin da ... | 2014 | 24858566 |
| structural basis for histone mimicry and hijacking of host proteins by influenza virus protein ns1. | pathogens can interfere with vital biological processes of their host by mimicking host proteins. the ns1 protein of the influenza a h3n2 subtype possesses a histone h3k4-like sequence at its carboxyl terminus and has been reported to use this mimic to hijack host proteins. however, this mimic lacks a free n-terminus that is essential for binding to many known h3k4 readers. here we show that the double chromodomains of chd1 adopt an 'open pocket' to interact with the free n-terminal amine of h3k ... | 2014 | 24853335 |
| protective efficacy of intranasally administered bivalent live influenza vaccine and immunological mechanisms underlying the protection. | previously we reported the generation of a new potential live attenuated influenza vaccine (laiv) named siv/606 that expresses h1 and h3 has. we also demonstrated intratracheal vaccination of siv/606 conferred protection against infections with both h1 and h3 swine influenza virus subtypes in pigs. here we vaccinated pigs with siv/606 intranasally, which is a more suitable route for laiv, and evaluated vaccine efficacy. intranasal vaccination of siv/606 induced serum igg antibody responses again ... | 2014 | 24837774 |
| the use of plethysmography in determining the severity of lung pathology in a mouse model of minimally lethal influenza virus infection. | to characterize the impact on lung function, we assessed plethysmography parameters in a course of infection with mouse-adapted a/pennsylvania/14/2010 (h3n2) influenza virus. several parameters, represented by enhanced pause (penh) and ratio of inspiratory/expiratory time (ti/te), were observed that had early (1-7dpi) and robust changes regardless of virus challenge dose. other parameters, characterized by tidal volume (tv), breathing frequency (freq) and end inspiratory pause (eip), changed lat ... | 2014 | 24837607 |
| comparison between virus shedding and fever duration after treating children with pandemic a h1n1/09 and children with a h3n2 with a neuraminidase inhibitor. | shedding of the pandemic virus during an influenza pandemic is thought to persist longer than shedding of influenza viruses during annual influenza seasons, because people have much less immunity against a pandemic influenza. a correlation is thought to exist between the length of virus shedding and the clinical severity of influenza illness. | 2015 | 24832015 |
| n-glycolylneuraminic acid on human epithelial cells prevents entry of influenza a viruses that possess n-glycolylneuraminic acid binding ability. | some animal influenza a viruses (iavs) bind not only to n-acetylneuraminic acid (neu5ac) but also to n-glycolylneuraminic acid (neu5gc), which has been discussed as a virus receptor. human cells cannot synthesize neu5gc due to dysfunction of the cmp-neu5ac hydroxylase (cmah) gene, which converts cmp-neu5ac to cmp-neu5gc. however, exogenous neu5gc from neu5gc-rich dietary sources is able to be metabolically incorporated into surfaces of tissue cells and may be related to enhancement of the infect ... | 2014 | 24829344 |
| effectiveness of seasonal influenza vaccinations against laboratory-confirmed influenza-associated infections among singapore military personnel in 2010-2013. | limited information is available about seasonal influenza vaccine effectiveness (ve) in tropical communities. | 2014 | 24828687 |
| incidence of influenza virus infections in children in hong kong in a 3-year randomized placebo-controlled vaccine study, 2009-2012. | school-aged children suffer high rates of influenza virus infections and associated illnesses each year, and are a major source of transmission in the community. however, information on the cumulative incidence of infection in specific epidemics is scarce, and there are limited studies with sufficient follow-up to identify the strength and duration of protection against reinfection. | 2014 | 24825868 |
| influenza virus genotypes circulating in and around lucknow, uttar pradesh, india, during post pandemic period, august 2010--september 2012. | during the post influenza pandemic period, continuous surveillance of influenza virus and its subtypes is mandatory to help the policy makers to take effective and appropriate decisions. therefore, this study was planned to determine the pattern of influenza virus activity in context to various meteorological and clinical parameters in and around lucknow, uttar pradesh, india, during post pandemic period august 2010 - september 2012. | 2014 | 24820836 |
| [determination of the frequency of human bocavirus and other respiratory viruses among 0-2 years age group children diagnosed as acute bronchiolitis]. | acute bronchiolitis, mostly seen in infants and younger children, is a lower respiratory tract infection frequently caused by viral agents. we aimed to determine the frequency of a broad panel of respiratory viruses including human bocavirus (hbov) and to assess the clinical characteristics of acute bronchiolitis in a group of children under 24 months of age. a total of 62 children (45 male, 17 female; age range: 0-2 years) with the initial diagnosis of acute bronchiolitis and 33 healthy childre ... | 2014 | 24819262 |
| comparison of the characteristics of elderly influenza patients in two consecutive seasons. | the seasonality of influenza infections can be affected by virus subtypes, climate, and social networking in populations. while these factors are well known, their relative influences in specific age groups have not been fully investigated. during 2010-2011, patients aged 65 years and above with influenza virus infections were recruited from a regional hospital in hong kong. they were either residents of homes for the elderly (n=60) or living with their family (n=75). two seasons were distinguis ... | 2014 | 24815741 |
| identification of four genotypes of h3n2 swine influenza virus in pigs from southern china. | in 2011, four h3n2 swine influenza viruses (sivs) were isolated from nasal swabs of four pigs (800 nasal swabs were collected from pigs showing influenza-like symptoms) in guangdong province, china. four different genotypes of h3n2 appeared among pigs in southern china, including wholly human-like h3n2 viruses, intermediate (1975) double-reassortant human h3n2 viruses (resulting from reassortment between an early human lineage and a recent human lineage), recent double-reassortant human h3n2 vir ... | 2014 | 24810098 |
| suppressor of cytokine signaling 4 (socs4) protects against severe cytokine storm and enhances viral clearance during influenza infection. | suppressor of cytokine signaling (socs) proteins are key regulators of innate and adaptive immunity. there is no described biological role for socs4, despite broad expression in the hematopoietic system. we demonstrate that mice lacking functional socs4 protein rapidly succumb to infection with a pathogenic h1n1 influenza virus (pr8) and are hypersusceptible to infection with the less virulent h3n2 (x31) strain. in socs4-deficient animals, this led to substantially greater weight loss, dysregula ... | 2014 | 24809749 |
| novel avian influenza a (h7n9) virus induces impaired interferon responses in human dendritic cells. | in march 2013 a new avian influenza a(h7n9) virus emerged in china and infected humans with a case fatality rate of over 30%. like the highly pathogenic h5n1 virus, h7n9 virus is causing severe respiratory distress syndrome in most patients. based on genetic analysis this avian influenza a virus shows to some extent adaptation to mammalian host. in the present study, we analyzed the activation of innate immune responses by this novel h7n9 influenza a virus and compared these responses to those i ... | 2014 | 24804732 |
| serological and virological surveillance of avian influenza a virus h9n2 subtype in humans and poultry in shanghai, china, between 2008 and 2010. | we report the serological evidence of low-pathogenic avian influenza (lpai) h9n2 infection in an occupational poultry-exposed population and a general population. a serological survey of an occupational poultry-exposed population and a general population was conducted using a haemagglutinin-inhibiting (hi) assay in shanghai, china, from january 2008 to december 2010. evidence of higher anti-h9 antibodies was found in serum samples collected from poultry workers. during this period, 239 h9n2 avia ... | 2015 | 24803167 |
| synthesis and anti-influenza virus activity of 4-oxo- or thioxo-4,5-dihydrofuro[3,4-c]pyridin-3(1h)-ones. | a target-free approach was applied to discover anti-influenza viral compounds, where influenza infected madin-darby canine kidney cells were treated 7500 different small organic chemicals individually and reduction of virus-induced cytopathic effect was measured. one of the hit compounds was (z)-1-((5-fluoro-1h-indol-3-yl)methylene)-6-methyl-4-thioxo-4,5-dihydrofuro[3,4-c]pyridin-3(1h)-one (15a) with half-maximal effective concentrations of 17.4-21.1μm against influenza a/h1n1, a/h3n2 and b viru ... | 2014 | 24794525 |
| aminoadamantanes with persistent in vitro efficacy against h1n1 (2009) influenza a. | a series of 2-adamantanamines with alkyl adducts of various lengths were examined for efficacy against strains of influenza a including those having an s31n mutation in m2 proton channel that confer resistance to amantadine and rimantadine. the addition of as little as one ch2 group to the methyl adduct of the amantadine/rimantadine analogue, 2-methyl-2-aminoadamantane, led to activity in vitro against two m2 s31n viruses a/calif/07/2009 (h1n1) and a/pr/8/34 (h1n1) but not to a third a/ws/33 (h1 ... | 2014 | 24793875 |
| population-based hospitalization burden of influenza a virus subtypes and antigenic drift variants in children in hong kong (2004-2011). | we aim to document and analyze influenza hospitalization burden in light of antigenic changes in circulating influenza viruses in hong kong. | 2014 | 24786780 |
| the rapid and sustained responses of dendritic cells to influenza virus infection in a non-human primate model. | dendritic cells (dcs) are readily infected by influenza viruses and play a crucial role in regulating host innate and adaptive immune responses to viral infection. the aims of this study are to characterize the dynamic changes in the numbers and maturation status of dendritic cells present in the lung and lung-associated lymph nodes (lalns) in the model of a non-human primate (nhp) infected by influenza a virus (iav). cynomolgus macaques were infected with influenza a virus (h3n2) via bronchosco ... | 2016 | 24780366 |
| genesis and pathogenesis of the 1918 pandemic h1n1 influenza a virus. | the source, timing, and geographical origin of the 1918-1920 pandemic influenza a virus have remained tenaciously obscure for nearly a century, as have the reasons for its unusual severity among young adults. here, we reconstruct the origins of the pandemic virus and the classic swine influenza and (postpandemic) seasonal h1n1 lineages using a host-specific molecular clock approach that is demonstrably more accurate than previous methods. our results suggest that the 1918 pandemic virus originat ... | 2014 | 24778238 |
| a zanamivir dimer with prophylactic and enhanced therapeutic activity against influenza viruses. | emerging drug resistance to antiviral therapies is an increasing challenge for the treatment of influenza virus infections. one new antiviral compound, bta938, a dimeric derivative of the viral neuraminidase inhibitor zanamivir, contains a 14-carbon linker bridging two zanamivir moieties. in these studies, we evaluated antiviral efficacy in cell cultures infected with influenza virus and in mouse models of lethal influenza using h1n1pdm09, h3n2 and oseltamivir-resistant (h275y) viruses. | 2014 | 24777908 |
| molecular characterization of circulating pandemic strains of influenza a virus during 2012 to 2013 in lucknow (india). | the pandemic h1n1 strain of influenza a virus [a(h1n1)pdm09] is now well adapted in human populations. however, it is still causing sporadic outbreaks worldwide with different severity. the present study was planned to understand the genetic diversity (based on the ha1 gene) of influenza a(h1n1)pdm09 strains circulating during the post pandemic period. the ha1 gene was selected because the ha1 protein is immunogenic, functions as a receptor binding site and indirectly affects transmission and pa ... | 2014 | 24777528 |
| performance of ehealth data sources in local influenza surveillance: a 5-year open cohort study. | there is abundant global interest in using syndromic data from population-wide health information systems--referred to as ehealth resources--to improve infectious disease surveillance. recently, the necessity for these systems to achieve two potentially conflicting requirements has been emphasized. first, they must be evidence-based; second, they must be adjusted for the diversity of populations, lifestyles, and environments. | 2014 | 24776527 |
| [immunization with live attenuated a/h5n1 vaccine protects guinea pigs from reinfection]. | cold-adapted influenza virus a/hk/1/68/162/35(h3n2) was developed as unified donor of attenuation and high reproductive capacity forvaccine strains. the reassortant of this donor with surface antigens of highly pathogenic strain alchicken/astana/6/05 (h5n1) was tested in guinea pigs as a live or inactivated preparation. immunization with both formulations induced equal levels of serum virus specific antibodies, while the level of mucosal antibodies was significantly higher in animals immunized w ... | 2016 | 24772645 |
| [evolution of influenza a(h3n2) virus from 2007 to 2012]. | the results of the genetic studies of influenza viruses make it possible to understand their evolution and recommendations for vaccine strains content. in this work, the data of complete sequence of the ha, na, and m2-protein for 34 strains of influenza a(h3n2) virus circulating in russia during 2007-2012 are presented. the influenza strains were isolated in ivanovsky institute of virology, moscow, and some collaborating russian centers. the results of the phylogenetic analysis showed the differ ... | 2016 | 24772643 |
| emergence of truncated pb1-f2 protein of h3n2 influenza virus during its epidemic period in jiangsu province, china. | pb1-f2 protein has been proven to increase the pathogenicity of influenza a virus (iav) strains in primary infection and in secondary bacterial infection. it can also regulate the activity of viral polymerase. however, it was shown in another retrospective study that a portion of iavs do not express full-length pb1-f2 protein during virus development; different kinds of stop codons cause exits in the open reading frames and form pb1-f2 gene products with the corresponding genotypes. truncated pb ... | 2014 | 24762594 |
| managing seasonal influenza: oseltamivir treatment policy in indonesia? | to manage cases of avian influenza a/h5n1 virus infection and in anticipation of a pandemic triggered by this virus, indonesia purchased and distributed oseltamivir to the government health facilities. oseltamivir is an antiviral drug that was developed for the treatment of influenza infections. disease surveillance and research suggests that seasonal influenza (a/h1n1, a/h3n2 or b) results in considerable morbidity and mortality in indonesia, where over 15% of influenza-like illness and severe ... | 2014 | 24760811 |
| akt inhibitor mk2206 prevents influenza ph1n1 virus infection in vitro. | the influenza ph1n1 virus caused a global flu pandemic in 2009 and continues manifestation as a seasonal virus. better understanding of the virus-host cell interaction could result in development of better prevention and treatment options. here we show that the akt inhibitor mk2206 blocks influenza ph1n1 virus infection in vitro. in particular, at noncytotoxic concentrations, mk2206 alters akt signaling and inhibits endocytic uptake of the virus. interestingly, mk2206 is unable to inhibit h3n2, ... | 2014 | 24752266 |
| influenza a subtype h3 viruses in feral swine, united states, 2011-2012. | to determine whether, and to what extent, influenza a subtype h3 viruses were present in feral swine in the united states, we conducted serologic and virologic surveillance during october 2011-september 2012. these animals were periodically exposed to and infected with a(h3n2) viruses, suggesting they may threaten human and animal health. | 2014 | 24751326 |
| reassortant swine influenza viruses isolated in japan contain genes from pandemic a(h1n1) 2009. | in 2013, three reassortant swine influenza viruses (sivs)-two h1n2 and one h3n2-were isolated from symptomatic pigs in japan; each contained genes from the pandemic a(h1n1) 2009 virus and endemic sivs. phylogenetic analysis revealed that the two h1n2 viruses, a/swine/gunma/1/2013 and a/swine/ibaraki/1/2013, were reassortants that contain genes from the following three distinct lineages: (i) h1 and nucleoprotein (np) genes derived from a classical swine h1 ha lineage uniquely circulating among ja ... | 2014 | 24750464 |
| molecular characterization and phylogenetic analysis of h3 subtype avian influenza viruses isolated from domestic ducks in zhejiang province in china. | in 2013, 15 avian influenza viruses (aivs), h3n2 (n = 7), h3n3 (n = 3), h3n6 (n = 3), and h3n8 (n = 2), were isolated from domestic ducks in zhejiang province in china. these strains were characterized by whole genome sequencing with subsequent phylogenetic analysis and genetic comparison. phylogenetic analysis of all eight viral genes showed that these strains clustered in the aiv eurasian lineage. analysis of the neuraminidase (na) gene indicates that a re-assortment event between h3 and h9n2 ... | 2014 | 24748106 |
| development of a reverse transcription loop-mediated isothermal amplification assay for the rapid diagnosis of avian influenza a (h7n9) virus infection. | a genetic diagnosis system for detecting avian influenza a (h7n9) virus infection using reverse transcription-loop-mediated isothermal amplification (rt-lamp) technology was developed. the rt-lamp assay showed no cross-reactivity with seasonal influenza a (h3n2 and h1n1pdm09) or influenza b viruses circulating in humans or with avian influenza a (h5n1) viruses. the sensitivity of the rt-lamp assay was 42.47 copies/reaction. considering the high specificity and sensitivity of the assay for detect ... | 2014 | 24747008 |
| substantial morbidity and mortality associated with pandemic a/h1n1 influenza in mexico, winter 2013-2014: gradual age shift and severity. | a recrudescent wave of pandemic influenza a/h1n1 is underway in mexico in winter 2013-14, following a mild 2012-13 a/h3n2 influenza season. mexico previously experienced several waves of pandemic a/h1n1 activity in spring, summer and fall 2009 and winter 2011-2012, with a gradual shift of influenza-related hospitalizations and deaths towards older ages. here we describe changes in the epidemiology of the 2013-14 a/h1n1 influenza outbreak, relative to previous seasons dominated by the a/h1n1 pand ... | 2014 | 24744975 |
| intrahost dynamics of influenza virus reassortment. | the segmented nature of the influenza virus genome allows reassortment between coinfecting viruses. this process of genetic exchange vastly increases the diversity of circulating influenza viruses. the importance of reassortment to public health is clear from its role in the emergence of a number of epidemiologically important viruses, including novel pandemic and epidemic strains. to gauge its impact on within-host genomic variation, we tracked reassortment in coinfected guinea pigs over time a ... | 2014 | 24741099 |
| safety and immunogenicity of a vero cell culture-derived whole-virus h5n1 influenza vaccine in chronically ill and immunocompromised patients. | the development of vaccines against h5n1 influenza a viruses is a cornerstone of pandemic preparedness. clinical trials of h5n1 vaccines have been undertaken in healthy subjects, but studies in risk groups have been lacking. in this study, the immunogenicity and safety of a nonadjuvanted cell culture-derived whole-virus h5n1 vaccine were assessed in chronically ill and immunocompromised adults. subjects received two priming immunizations with a clade 1 a/vietnam h5n1 influenza vaccine, and a sub ... | 2014 | 24739978 |
| analysis of the acute-phase protein response in pigs to clinical and subclinical infection with h3n2 swine influenza virus. | swine influenza (si) is a contagious, important respiratory disease. diagnosis of si is based on the clinical signs, confirmed by the detection of viral rna or specific antibodies. however, the infection is much more frequent than the disease. | 2014 | 24734294 |
| influenza h1n1pdm-specific maternal antibodies offer limited protection against wild-type virus replication and influence influenza vaccination in ferrets. | the objective was to study passively acquired influenza h1n1 pandemic (h1n1pdm) maternal antibody kinetics and its impact on subsequent influenza infection and vaccination in ferrets during an outbreak of the h1n1pdm. | 2014 | 24734293 |
| duration of the protective immune response after prime and booster vaccination of yearlings with a live modified cold-adapted viral vaccine against equine influenza. | we previously created a live vaccine against equine influenza based the new reassortant cold-adapted (ca) strain a/hk/otar/6:2/2010. the live vaccine contains surface proteins (ha, na) from the wild-type virus a/equine/otar/764/2007 (н3n8; american lineage florida clade 2), and internal proteins (pb2, pb1, pa, np, m, ns) from the attenuated ca donor virus a/hong kong/1/68/162/35ca (h3n2). to determine the safety and duration of the protective immune responses, 90 yearlings were intranasally vacc ... | 2014 | 24726250 |
| assessment of seasonality of influenza in swine using field submissions to a diagnostic laboratory in ontario between 2007 and 2012. | seasonality of any infectious disease is important for its control and monitoring. while influenza seasonality in people has been evaluated extensively, this question has not been studied well in swine populations. | 2014 | 24725968 |
| conserved neutralizing epitope at globular head of hemagglutinin in h3n2 influenza viruses. | neutralizing antibodies that target the hemagglutinin of influenza virus either inhibit binding of hemagglutinin to cellular receptors or prevent the low-ph-induced conformational change in hemagglutinin required for membrane fusion. in general, the former type of antibody binds to the globular head formed by ha1 and has narrow strain specificity, while the latter type binds to the stem mainly formed by ha2 and has broad strain specificity. in the present study, we analyzed the epitope and funct ... | 2014 | 24719430 |
| receptor mimicry by antibody f045-092 facilitates universal binding to the h3 subtype of influenza virus. | influenza viruses present a significant health challenge each year, as in the h3n2 epidemic of 2012-2013. here we describe an antibody, f045-092, that possesses broadly neutralizing activity against the entire h3 subtype and accommodates the natural variation and additional glycosylation in all strains tested from 1963 to 2011. crystal structures of f045-092 in complex with has from 1975 and 2011 h3n2 viruses reveal the structural basis for its neutralization breadth through insertion of its 23- ... | 2014 | 24717798 |
| epidemiological aspects of influenza a related to climatic conditions during and after a pandemic period in the city of salvador, northeastern brazil. | during the influenza pandemic of 2009, the a(h1n1)pdm09, a/h3n2 seasonal and influenza b viruses were observed to be co-circulating with other respiratory viruses. to observe the epidemiological pattern of the influenza virus between may 2009-august 2011, 467 nasopharyngeal aspirates were collected from children less than five years of age in the city of salvador. in addition, data on weather conditions were obtained. indirect immunofluorescence, real-time transcription reverse polymerase chain ... | 2014 | 24714967 |
| innate immune response to a h3n2 subtype swine influenza virus in newborn porcine trachea cells, alveolar macrophages, and precision-cut lung slices. | viral respiratory diseases remain of major importance in swine breeding units. swine influenza virus (siv) is one of the main known contributors to infectious respiratory diseases. the innate immune response to swine influenza viruses has been assessed in many previous studies. however most of these studies were carried out in a single-cell population or directly in the live animal, in all its complexity. in the current study we report the use of a trachea epithelial cell line (newborn pig trach ... | 2014 | 24712747 |
| prevalence and correlates of influenza-a in piggery workers and pigs in two communities in lagos, nigeria. | worldwide, three influenza-a virus subtypes (h1n1, h1n2 and h3n2) in swine are major public health issues. in nigeria, the existence of these subtypes in pigs has not been well studied. this study aimed at determining the prevalence and correlates of influenza-a viruses circulating in piggery workers and pigs in oke-aro and goshen communities in lagos, nigeria. | 2013 | 24711881 |
| il-15 adjuvanted multivalent vaccinia-based universal influenza vaccine requires cd4+ t cells for heterosubtypic protection. | current influenza vaccines are ineffective against novel viruses and the source or the strain of the next outbreak of influenza is unpredictable; therefore, establishing universal immunity by vaccination to limit the impact of influenza remains a high priority. to meet this challenge, a novel vaccine has been developed using the immunogenic live vaccinia virus as a vaccine vector, expressing multiple h5n1 viral proteins (ha, na, m1, m2, and np) together with il-15 as a molecular adjuvant. previo ... | 2014 | 24706798 |
| mutations flanking the carbohydrate binding site of surfactant protein d confer antiviral activity for pandemic influenza a viruses. | we recently reported that a trimeric neck and carbohydrate recognition domain (ncrd) fragment of human surfactant protein d (sp-d), a host defense lectin, with combinatorial substitutions at the 325 and 343 positions (d325a+r343v) exhibits markedly increased antiviral activity for seasonal strains of influenza a virus (iav). the ncrd binds to glycan-rich viral envelope proteins including hemagglutinin (ha). we now show that replacement of d325 with serine to create d325s+r343v provided equal or ... | 2014 | 24705721 |
| a humoral immunity survey following the 2012 influenza season after the ph1n1 pandemic in guangzhou, china. | according to pathogenic surveillance data during the first half of 2012, the h3n2 influenza virus was prevalent in guangdong, china, but no pandemic h1n1 (ph1n1) virus was detected. this study aimed to measure the seroprevalence of ph1n1 and h3n2 infection following the influenza epidemic in 2012. we collected serum samples by stratified random sampling in a cross-sectional survey from august, 2012 to october, 2012. antibody titers against h3n2, ph1n1, and influenza b antigens were measured by t ... | 2014 | 24702517 |