Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (bubalus bubalis) mammary epithelial cell line. | the aim of the present study was construction of mammary gland specific expression vector for high level of human insulin (hins) expression in transgenic buffalo for therapeutic use. we have constructed mammary gland specific vector containing human insulin gene and there expression efficiency was checked into in vitro cultured buffalo mammary epithelial cells (bumecs). human pro-insulin coding region was isolated from human genomic dna by intron skipping pcr primer and furin cleavage site was i ... | 2014 | 24969480 |
| detection of sex chromosome aneuploidy in dog spermatozoa by triple color fluorescence in situ hybridization. | with the development of a direct visualization of sex chromosome in a single sperm by fluorescence in situ hybridization (fish) technique, the frequency of aberration (aneuploidy) in spermatozoa in several mammals has been investigated. however, there is no report in the incidence of x-y aneuploidy in the sperm population of dogs. therefore, in this study, the aneuploidy in dog spermatozoa was examined by multicolor fish using specific molecular probes for canine sex chromosomes and autosome. se ... | 2014 | 24962971 |
| first report of isolation and molecular characterization of bubaline herpesvirus 1 (buhv1) from argentinean water buffaloes. | herpesviruses have mainly co-evolved with their hosts for millions of years. however, bovine herpesvirus 1 (bohv1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. bubaline herpesvirus 1 (buhv1) is an alphaherpesvirus closely related to bohv1 and bohv5. according to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of bohv1-related virus infection in wild and domestic ruminant species. rece ... | 2014 | 24938487 |
| developmental competence and expression pattern of bubaline (bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro. | to determine the direct effect of physiologically relevant high temperatures (40.5 and 41.5 °c) for two time periods (12 and 24 h) on bubaline oocytes during in vitro maturation. | 2014 | 24938361 |
| detection of copy number variations and their effects in chinese bulls. | copy number variations (cnvs) are a main source of genomic structural variations underlying animal evolution and production traits. here, with one pure-blooded angus bull as reference, we describe a genome-wide analysis of cnvs based on comparative genomic hybridization arrays in 29 chinese domesticated bulls and examined their effects on gene expression and cattle growth traits. | 2014 | 24935859 |
| identification of fluoroquinolone-resistant extended-spectrum β-lactamase (ctx-m-8)-producing escherichia coli st224, st2179 and st2308 in buffalo (bubalus bubalis). | 2014 | 24928853 | |
| evaluation of antifreeze protein iii for cryopreservation of nili-ravi (bubalus bubalis) buffalo bull sperm. | lower fertility in buffaloes with frozen-thawed semen is attributed to sperm damage that is believed to be due to formation of ice crystals during freeze/thaw process. it was hypothesized that antifreeze proteins in the extender may improve the post thaw quality of buffalo bull sperm. for this purpose, two separate experiments were conducted to evaluate antifreeze proteins iii (afp iii) at 0 (control), 0.1, 1 and 10 μg/ml (experiment i) and 0 (control), 0.01, 0.1 and 1 μg/ml (experiment ii) for ... | 2014 | 24925471 |
| effects of trichostatin a on in vitro development and dna methylation level of the satellite i region of swamp buffalo (bubalus bubalis) cloned embryos. | trichostatin a (tsa), a histone deacetylase inhibitor, has been widely used to improve the cloning efficiency in several species. this brings our attention to investigation of the effects of tsa on developmental potential of swamp buffalo cloned embryos. swamp buffalo cloned embryos were produced by electrical pulse fusion of male swamp buffalo fibroblasts with swamp buffalo enucleated oocytes. after fusion, reconstructed oocytes were treated with 0, 25 or 50 nm tsa for 10 h. the results showed ... | 2014 | 24909601 |
| leuconostoc mesenteroides sjrp55: a bacteriocinogenic strain isolated from brazilian water buffalo mozzarella cheese. | the production of bacteriocins by leuconostoc mesenteroides represents an important opportunity for exploration of their potential use for industrial purpose. the antimicrobial compounds produced by l. mesenteroides subsp. mesenteroides sjrp55 strain were characterized and purified. cell-free supernatant of leuc. mesenteroides subsp. mesenteroides sjrp55 produced antibacterial compounds against listeria spp. strains and not inhibiting against lactobacillus spp. the antimicrobial substances were ... | 2014 | 24907159 |
| morphogenesis of mammary glands in buffalo (bubalus bubalis). | the present research was elucidated on the morphogenesis of mammary gland of buffalo during prenatal development. total of 16 foetuses ranging from 1.2 cm (34 days) to 108 cm cvrl (curved crown rump length) (317 days) were used for study. the study revealed that mammary line was first observed at 1.2 cm cvrl (34 days), mammary hillock at 1.7 cm (37 days), and mammary bud at 2.6 cm cvrl (41 days) foetuses. epidermal cone was found at 6.7 cm cvrl (58 days) whereas primary and secondary ducts were ... | 2014 | 24876967 |
| molecular cloning and characterization of a novel heat shock protein 20 of babesia orientalis. | the heat shock protein 20 (hsp20) gene of babesia orientalis (bohsp20) was identified from both genomic dna and cdna. the full-length bohsp20 gene was 690bp with one intron from position 88-243bp. the amplicon obtained from cdna corresponded to a full-length open reading frame (orf) with a length of 534bp, encoding a polypeptide of 178 amino acid residues with a predicted size of 20kda. the orf was cloned into a pet-28a plasmid and subsequently expressed as a his-fusion protein. the recombinant ... | 2014 | 24857770 |
| transmission of foot-and-mouth disease virus from experimentally infected indian buffalo (bubalus bubalis) to in-contact naïve and vaccinated indian buffalo and cattle. | this study investigated the transmission of foot-and-mouth disease virus (fmdv) from experimentally infected indian buffalo to in-contact naïve and vaccinated cattle and buffalo. in each of six rooms, two donor buffalo that had been inoculated with fmdv were housed for five days with four recipient animals, comprising one vaccinated buffalo, one vaccinated calf, one unvaccinated buffalo and one unvaccinated calf. vaccination was carried out with current indian vaccine strain (o/ind/r2/75) and ch ... | 2014 | 24837776 |
| energy metabolites, lipid variables and lactation performance of periparturient murrah buffaloes (bubalus bubalis) fed on diet supplemented with inorganic chromium. | the aim of this study was to elucidate the effects of chromium (cr) supplementation as inorganic cr (crcl3·6h2o) on energy balance, lipid peroxidation, and lactation performance in periparturient murrah buffaloes. twenty-four multiparous murrah buffaloes according to lactation, parity, body mass, and expected calving date were divided equally. experimental buffaloes were randomly assigned to four treatment diets: a control diet and three diets with an inorganic cr supplementation at 0.5, 1.0, an ... | 2014 | 24833279 |
| serum biochemical and haematological reference intervals for water buffalo bubalus bubalis heifers. | based on a review of the literature, reference intervals for water buffalo (bubalus bubalis) serum biochemistry and haematology have not previously been published. the current study was done to establish reference intervals for water buffalo heifers. the international federation of clinical chemistry stated that at least 120 values are necessary to obtain reliable estimates for reference intervals. a total number of 127 clinically healthy buffalo heifers (1-2 years old) were included in the stud ... | 2014 | 24831856 |
| histology and ultrastructure of the lymph nodes of the buffalo (bos bubalus). | pre-scapular, femoral and mesenteric lymph nodes from five buffalo calves and five buffalo bulls were studied using light and transmission electron microscopy. the nodes were surrounded with a thin capsule of dense connective tissue and smooth muscles. subcapsular and trabecular lymphatic sinuses were lined with endothelial cells resting on a basement membrane. the cortex was formed by lymphoid follicles and inter-follicular lymphocytes. primary and secondary follicles were observed. the medulla ... | 2015 | 24810142 |
| first cloned swamp buffalo produced from adult ear fibroblast cell. | the world's first cloned swamp buffalo (bubalus bubalis) derived from adult ear skin fibroblast has been reported. donor fibroblast cells were produced from biopsies taken from adult male ear skin and in vitro matured oocytes obtained from a slaughterhouse were used as cytoplasts. a total of 39 blastocysts and 19 morulae fresh embryos were transferred into 12 recipient buffaloes. progesterone assays indicated establishment of pregnancy in 10 of the 12 buffaloes (83.3%) after 45 days, with six an ... | 2014 | 24804579 |
| neospora caninum serostatus is affected by age and species variables in cohabiting water buffaloes and beef cattle. | the aim of this study was to investigate how neospora caninum serostatus may be affected by variables such as host species (water buffaloes or cattle) and age in animals cohabiting in the same ranch. a convenience cross-sectional study was performed on four ranches in the northeast of argentina, where water buffalo are cohabitating with beef cattle. blood samples were collected from 1350 female water buffaloes (bubalus bubalis) and 880 female beef cattle (bos taurus and bos indicus crossbreeds) ... | 2014 | 24792747 |
| effects of melatonin and epiphyseal proteins on fluoride-induced adverse changes in antioxidant status of heart, liver, and kidney of rats. | several experimental and clinical reports indicated the oxidative stress-mediated adverse changes in vital organs of human and animal in fluoride (f) toxicity. therefore, the present study was undertaken to evaluate the therapeutic effect of buffalo (bubalus bubalis) epiphyseal (pineal) proteins (bep) and melatonin (mel) against f-induced oxidative stress in heart, liver, and kidney of experimental adult female rats. to accomplish this experimental objective, twenty-four adult female wistar rats ... | 2014 | 24790596 |
| identification of three novel myeloid cathelicidin cdnas and their predicted peptides in buffalo (bubalus bubalis). | antimicrobial peptides (amps) are broad spectrum antibiotics, which mostly act without specific receptors. identification of amps is important in the current scenario of emerging multi-drug resistant bacteria. in the present study, in an attempt to identify new amps, myeloid cathelicidin cdnas were synthesized from buffalo (bubalus bubalis) bone marrow and were amplified using specific primers. sequence analysis of cloned cdnas revealed three novel myeloid cathelicidins. they were named based on ... | 2013 | 24772945 |
| handling and welfare of bovine livestock at local abattoirs in bangladesh. | the world organization for animal health (oie) allows rope casting and the tying of legs for nonhuman animal slaughter without stunning. the handling and welfare of bovine livestock (bos indicus and bubalus bubalis) were studied in 8 local abattoirs in 5 districts of bangladesh. a total of 302 animals were evaluated. at the local abattoirs, approximately 1/3 of the cattle and water buffalo were either emaciated or injured/sick. the size and vigor of the animals determined the casting method. sma ... | 2014 | 24766081 |
| association of a novel snp in exon 10 of the igf2 gene with growth traits in egyptian water buffalo (bubalus bubalis). | insulin-like growth factor 2 (igf2) plays an important role in muscle growth and it might be used as a marker for the growth traits selection strategies in farm animals. the objectives of this study were to detect polymorphisms in exon 10 of igf2 and to determine associations between these polymorphisms and growth traits in egyptian water buffalo. pcr-single-strand conformation polymorphism (sscp) and dna sequencing methods were used to detect any prospective polymorphism. a novel single nucleot ... | 2014 | 24756464 |
| sequence characterization, polymorphism, and tissue expression profile of an effector immediate-early gene: activity-regulated cytoskeletal associated protein gene (arc/arg3.1) in swamp and river buffalo. | the activity-regulated cytoskeletal associated protein (arc/arg3.1) has been implicated in experience-dependent synaptic plasticity and memory formation. however, information regarding its coding gene in buffalo remains scarce. in this study, the full-length of arc/arg3.1 was isolated and characterized (accession no. jx491649) and genetic variations of six river buffalo and eight swamp buffalo were investigated. a tissue expression profile was obtained using semi-quantitative reverse transcripti ... | 2014 | 24737478 |
| preliminary molecular characterizations of sarcoptes scaibiei (acari: sarcoptidae) from farm animals in egypt. | little is known about the genetic diversity of sarcoptes scabiei mites in farm animals in egypt. in this study, we characterized s. scabiei in 25 skin scrapes from water buffalo, cattle, sheep, and rabbits at the nuclear marker its2 and mitochondrial markers cox1 and 16s rrna. sequences of the its2 showed no host segregation or geographical isolation, whereas those of the mitochondrial cox1 and 16s rrna genes indicated the presence of both host-adapted and geographically segregated populations o ... | 2014 | 24728386 |
| ontogenic development of corticotrophs in fetal buffalo (bubalus bubalis) pituitary gland. | to evaluate the subpopulation of corticotrophs in developing buffalo (bubalus bubalis) fetus, recovered pituitary glands (n=6 per group) from late first, second and third gestational female buffalo dams. the corticotrophs were identified by using specific antibodies against proopiomelanocortin (pomc) and adrenocorticotrophic hormone (acth) through immunohistochemistry. there was a significant (p≤0.05) increase of immunoreactive (ir) ir-acth cells during late 2nd trimester while, ir-pomc cells we ... | 2014 | 24704996 |
| effect of leptin on in vitro nuclear maturation and apoptosis of buffalo (bubalus bubalis) oocyte. | leptin, as a 16 kda adipokine, is a pleiotropic cytokine-like hormone that primarily secreted from adipose tissue. it also involves in the regulation of energy homeostasis, neuroendocrine function, immunity, lipid and glucose homeostasis, fatty acid oxidation, angiogenesis, puberty and reproduction. the aim of this study was to investigate the effects of in vitro addition of leptin to in vitro maturation (ivm) medium on buffalo oocyte maturation and apoptosis. | 2014 | 24696768 |
| haematological alterations induced by oral subacute exposure to fenvalerate, nitrate and their combination in domestic buffalo, bubalus bubalis. | the present study investigated haematological alterations induced by oral subacute exposure to fenvalerate, nitrate and their combination in the domestic buffalo, bubalus bubalis. fenvalerate exposure produced significant declines in haemoglobin (hb), total leukocyte count (tlc), total erythrocyte count (tec) and mean corpuscular haemoglobin concentration (mchc), and a corresponding elevation in mean corpuscular volume (mcv). following oral exposure to sodium nitrate, significant declines in blo ... | 2014 | 24687223 |
| bovine calves as ideal bio-indicators for fluoridated drinking water and endemic osteo-dental fluorosis. | relative susceptibility to fluoride (f) toxicosis in the form of osteo-dental fluorosis was observed in an observational survey of 2,747 mature and 887 immature domestic animals of diverse species living in areas with naturally fluoridated (>1.5 ppm f) drinking water. these animals included buffaloes (bubalus bubalis), cattle (bos taurus), camels (camelus dromedarius), donkeys (equus asinus), horses (equus caballus), goats (capra hircus), and sheep (ovis aries). of these mature and immature anim ... | 2014 | 24671615 |
| lead and trace element levels in milk and blood of buffaloes (bubalus bubalis) from hyderabad, india. | the presence of lead (pb) in milk and its interaction with trace elements is a serious health concern. present study is aimed at determining pb and trace element (fe, zn and mg) levels in milk and blood/serum samples of lactating buffaloes (bubalus bubalis) living in a market-area (group-a) and a dairy-experimental station (group-b), hyderabad, india. in addition, kidney and liver function tests were assessed. fodder, milk and blood pb levels were significantly (p < 0.01) higher in group-b. elev ... | 2014 | 24671305 |
| stage specific expression of atp-binding cassette and solute carrier superfamily of transporter genes in mammary gland of riverine buffalo (bubalus bubalis). | in the present study, expression level of various atp-binding cassette (abc) viz., abca1, abca7, abcg1, abcg2, and abcg5; associated transcription factors viz., srebf1, lxrα (nr1h3), ppara, and solute carriers (slc); or glucose transporters (glut) viz., slc2a1(glut1), slc2a4 (glut4), slc2a8 (glut8), and slc2a12 (glut12) superfamily of transporters were compared across physiological stages of buffalo mammary gland. the relative expression of abca1, and abcg1 was significantly (p < 0.05) higher in ... | 2014 | 24669870 |
| seroprevalence of flaviviruses antibodies in water buffaloes (bubalus bubalis) in brazilian amazon. | the state of pará encompasses 26% of brazilian amazon where an enormous diversity of arboviruses has been found. this study aimed to assess the prevalence and distribution of hemagglutination-inhibition antibodies against antigens of six flavivirus (yellow fever virus, ilheus virus, saint louis encephalitis virus, cacipacore virus, bussuquara virus and rocio virus) in water buffaloes in pará state, brazil. the prevalence of antibodies in these farm animals is important to determine the circulati ... | 2014 | 24666635 |
| development of a sequential multicolor-fish approach with 13 chromosome-specific painting probes for the rapid identification of river buffalo (bubalus bubalis, 2n = 50) chromosomes. | the development of new molecular techniques (array cgh, m-fish, sky-fish, etc.) has led to great advancements in the entire field of molecular cytogenetics. however, the application of these methods is still very limited in farm animals. in the present study, we report, for the first time, the production of 13 river buffalo (bubalus bubalis, 2n = 50) chromosome-specific painting probes, generated via chromosome microdissection and the dop-pcr procedure. a sequential multicolor-fish approach is a ... | 2014 | 24664789 |
| testicular lesions and epididymal sperm parameters in the iranian river buffalo (bubalus bubalis). | the aim of this study was to evaluate the testicular lesions and their effects on the epididymal sperm parameters in the iranian river buffalo (bubalus bubalis). total numbers of 117 scrota from the pubertal buffalo were provided from the local slaughterhouse. the samples were evaluated for morphological parameters and any macro- or microscopic lesions. the sterile swabs from the testis parenchyma were subjected to microbiology culture. the epididymal spermatozoon was analysed for concentration, ... | 2015 | 24661073 |
| mammary diffuse fibroadenomatoid hyperplasia in water buffalo (bubalus bubalis): three cases. | the current report describes 3 rare cases of mammary diffuse fibroadenomatoid hyperplasia in water buffalo (bubalus bubalis). all of the animals were between 10 and 12 months of age. grossly, the lesions consisted of severe diffuse swelling with homogeneous large masses in the udder. surgical removal of the masses was curative. microscopically, there was severe hyperplasia of the mammary epithelium and numerous well-differentiated and mildly pleomorphic acini and their associated ducts. moderate ... | 2014 | 24621849 |
| prevalence and molecular characterization of sarcocystis species in water buffaloes (bubalus bubalus) in egypt. | the present study was planned to investigate the prevalence of sarcocystis spp. among slaughtered water buffaloes (bubalus bubalis) at alexandria province, egypt. three hundred blood samples were collected from slaughtered buffaloes (5-7 years old). two techniques were used to evaluate the seroprevalence of sarcocystis spp., enzyme-linked immunosorbent assay (elisa) and indirect haemagglutination assay (iha). it was revealed that 203 (67.6 %) and 191 (63.6 %) of the tested serum samples were ser ... | 2014 | 24619513 |
| synchronization of follicular wave emergence following ultrasound-guided transvaginal follicle ablation or estradiol-17β administration in water buffalo (bubalus bubalis). | the aim of this study was to assess the synchrony in follicular wave emergence and subsequent ovulation following dominant follicle ablation or estradiol-17β administration. six cycling murrah buffaloes were sequentially allotted to three groups, that is, control, follicular ablation, and estradiol-17β groups. for the control group, buffaloes at random stages of estrous cycle were examined daily by transrectal ultrasonography for 14 days and the day of wave emergence was recorded. following indu ... | 2014 | 24612954 |
| effect of extender and equilibration time on post thaw motility and chromatin structure of buffalo bull (bubalus bubalis) spermatozoa. | the aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or bioxcell®) on cryopreservation of buffalo semen. | 2014 | 24611139 |
| reprogramming of buffalo (bubalus bubalis) foetal fibroblasts with avian egg extract for generation of pluripotent stem cells. | an attempt was made to study the efficiency of chicken (gallus gallus domesticus) egg extract (ee) to reprogram buffalo (bubalus bubalis) foetal fibroblasts (bffs) without incorporation of ectopic transcription factors. the isolated bffs were cultured in media supplemented with 2%, 4%, 6% and 10% ee to induce reprogramming. it was observed that fewer but larger sized alkaline phosphatase positive (ap(+ve)) colonies developed in culture system containing 2% ee whereas, more but smaller sized colo ... | 2014 | 24602917 |
| insight into buffalo (bubalus bubalis) rig1 and mda5 receptors: a comparative study on dsrna recognition and in-vitro antiviral response. | rig1 and mda5 have emerged as important intracellular innate pattern recognition receptors that recognize viral rna and mediate cellular signals controlling type i interferon (ifn-i) response. buffalo rig1 and mda5 genes were investigated to understand the mechanism of receptor induced antiviral response. sequence analysis revealed that rig1 and mda5 maintain a domain arrangement that is common in mammals. critical binding site residues of the receptors are evolutionary conserved among mammals. ... | 2014 | 24587036 |
| mitochondrial genome of babesia orientalis, apicomplexan parasite of water buffalo (bubalus babalis, linnaeus, 1758) endemic in china. | apicomplexan parasites of the genus babesia, theileria and plasmodium are very closely related organisms. interestingly, their mitochondrial (mt) genomes are highly divergent. among babesia, babesia orientalis is a new species recently identified and specifically epidemic to the southern part of china, causing severe disease to water buffalo. however, no information on the mt genome of b. orientalis was available. | 2014 | 24580772 |
| pcr detection of neospora caninum in water buffalo foetal tissues. | the seroprevalence of neospora caninum was surveyed by an elisa kit on two water buffalo herds of southern italy. seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. tissue samples collected from three aborted fetuses from the same herds were investigated for n. caninum presence by pcr assays targeting the 18s and the nc5 dna sequences, respectiv ... | 2014 | 24570043 |
| short communication: effects of systemic treatment with penethamate hydriodide on udder health and milk yields in dry primiparous mediterranean buffaloes (bubalus bubalis). | the effects of penethamate hydriodide (mamyzin, boehringer ingelheim, ingelheim, germany) on udder health and milk yields were evaluated in primiparous mediterranean buffaloes (bubalus bubalis). an intramuscular administration of 10 million international units was performed in 20 buffaloes at 7 d precalving (treatment group; tg), and 20 animals were enrolled as the control group (cg). evening milk samplings were performed at 10, 30, and 60 d in milk (dim). somatic cell count (scc) values were ev ... | 2014 | 24565324 |
| first molecular characterization of cryptosporidium and giardia from bovines (bos taurus and bubalus bubalis) in sri lanka: unexpected absence of c. parvum from pre-weaned calves. | the genetic characterization of cryptosporidium and giardia has important implications for investigating their epidemiology and underpins their control. we undertook the first molecular epidemiological survey of domestic bovids in selected regions of sri lanka to establish whether they excreted cryptosporidium and/or giardia with zoonotic potential. | 2014 | 24559043 |
| transcriptional profile of endometrial tlr4 and 5 genes during the estrous cycle and uterine infection in the buffalo (bubalus bubalis). | endometritis is one of the leading causes of infertility in the cattle and buffalo and innate immune mechanism plays an important role in clearing the infection. in this regard, endometrial expression and function of toll like receptors (tlr) are focus of investigation in the recent years. in this study, we report the transcriptional profiles of tlr4 and 5 in the buffalo endometrium during the follicular, early, mid and late luteal phases of estrous cycle and 'subclinical and clinical endometrit ... | 2014 | 24531997 |
| characterization of immune cell infiltration in the placentome of water buffaloes (bubalus bubalis) infected with neospora caninum during pregnancy. | neospora caninum infection in cattle stimulates host immune responses, which may be responsible for placental damage leading to abortion. susceptibility of water buffaloes (bubalus bubalis) to neosporosis is not well understood, although vertical transmission and fetal death have been documented. the aim of this study was to characterize the immune response in the placentome of water buffalo following experimental infection in early gestation with the nc-1 strain of n. caninum. placentomes were ... | 2014 | 24529512 |
| diagnostic value of the recombinant tandem repeat antigen tegm6-4r for surra in water buffaloes. | trypanosoma evansi infection, or surra, is currently affecting various species of animals, especially water buffaloes. since diagnosis is an important aspect of surra control, development of novel diagnostic antigens is of interest to implement and improve the currently utilized methods. our study evaluated the tandem repeat antigen tegm6-4r in t. evansi antibody detection in water buffaloes. tegm6-4r-based elisa was performed with 20 positive and 8 negative controls and 484 field samples from w ... | 2014 | 24524896 |
| high prevalence of muscular sarcocystosis in cattle and water buffaloes from selangor, malaysia. | the prevalence of sarcocystosis in cattle and water buffaloes from peninsular malaysia was investigated in abattoirs in selangor state, february, 2011, to march, 2012. fresh muscle samples were collected from the tongue, heart, oesophagus, diaphragm and skeletal muscles of 102 cattle and 18 water buffaloes. each sample was initially screened by light microscopy and then fixed for further histopathological analysis. out of 120 animals examined, 49 (40.8%) harboured the microscopic type of sarcocy ... | 2013 | 24522140 |
| yeasts and hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. | the aim of this work was to study the yeast populations and the main hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. forty-two water buffalo mozzarella samples were purchased from retail outlets in belo horizonte. in addition, five samples of consecutive starter cultures, curd before acidification, acidified curd and mozzarella were collected at an industry in the city of oliveira. only three of the five water samples analyz ... | 2013 | 24516436 |
| prevalence of infection and molecular confirmation by using its-2 region of fasciola gigantica found in domestic cattle from chiang mai province, thailand. | to investigate the infection of fasciola gigantica (f. gigantica) in domestic cattle from chiang mai province and molecular confirmation using its-2 region. | 2014 | 24507641 |
| prevalence and etiology of subclinical mastitis among buffaloes (bubalus bubalus) in namakkal, india. | milk samples from 206 apparently healthy buffaloes of marginal farmers maintained under the prevailing field conditions were screened for subclinical mastitis (scm) to determine its prevalence and etiology by white side test (wst), california mastitis test (cmt), somatic cell count (scc) and bacteriological examination. 26.20% of animals were positive for scm in scc and bacteriological examination. prevalence in graded murrah buffaloes were found to be higher compared to graded surti and non-des ... | 2013 | 24506047 |
| production of wild buffalo (bubalus arnee) embryos by interspecies somatic cell nuclear transfer using domestic buffalo (bubalus bubalis) oocytes. | the objective of this study was to explore the possibility of producing wild buffalo embryos by interspecies somatic cell nuclear transfer (iscnt) through handmade cloning using wild buffalo somatic cells and domestic buffalo (bubalus bubalis) oocytes. somatic cells derived from the ear skin of wild buffalo were found to express vimentin but not keratin and cytokeratin-18, indicating that they were of fibroblast origin. the population doubling time of skin fibroblasts from wild buffalo was signi ... | 2014 | 24494649 |
| gastrointestinal parasitic infection in diverse species of domestic ruminants inhabiting tribal rural areas of southern rajasthan, india. | a total of 415 adult domesticated ruminants, 130 cattle (bos taurus), 108 buffaloes (bubalus bubalis), 94 goats (capra hircus) and 83 sheep (ovis aries) inhabiting tribal rural areas of southern rajasthan, india were investigated for evidence of gastrointestinal protozoan and helminthic infections. in southern rajasthan humid ecosystem is predominant and has number of perennial freshwater bodies. fresh faecal samples of these animals were examined microscopically by direct wet smear with saline ... | 2012 | 24431582 |
| a rapid method for authentication of buffalo (bubalus bubalis) meat by alkaline lysis method of dna extraction and species specific polymerase chain reaction. | buffalo (bubalus bubalis) meat is a major item of export from india but export of beef i.e. meat from cattle (bos indicus) is prohibited. also, adulteration of buffalo meat with that of beef (meat from cattle) is a common fraudulent practice because of prohibition on cow slaughter in most states of india. food analysts require precise identification techniques to implement such regulations. in the present study, a method of dna extraction by alkaline lysis from meat samples and speciation of buf ... | 2011 | 24425899 |
| pathology of experimental infection by pasteurella multocida serotype a: 1 in buffalo calves. | pasteurella multocida serotype a:3 has been mostly implicated in pneumonic pasteurellosis in ruminants. in contrast, our previous studies have reported that both serotypes a:1 and a:3 were responsible for respiratory diseases in cattle and buffaloes. however, the pathology and pathogenesis of p. multocida serotype a:1 (pm a:1) infection have not been studied in ruminants. in the present study, 12- to 15-week-old buffalo calves (bubalus bubalis) infected by pm a:1 had fibrinous and suppurative br ... | 2014 | 24407559 |
| analysis of slc11a1 gene expression in healthy water buffalo (bubalus bubalis) blood cells using qpcr. | slc11a1 (solute carrier family 11 member 1 protein) gene influences the initial phase of bacterial cellular infections through macrophage activation. recent literature on buffalo has attempted to associate the genotype of the polymorphic microsatellite located in the 3'untranslated region (3'utr) of the gene, with either susceptibility to brucellosis or with improved macrophage function. carriers of the (gt)16 allele have been reported to be resistant to brucellosis. in this study we analyzed th ... | 2013 | 24391044 |
| glutathione-s-transferase: role in buffalo (bubalus bubalis) sperm capacitation and cryopreservation. | in this study, glutathione-s-transferase mu3 (gst) has been reported to play an important role in sperm capacitation, acrosome reaction, and fertilization. the freshly ejaculated buffalo spermatozoa were in vitro capacitated using heparin (10 μg/ml) or cryopreserved in egg yolk citrate extender. glutathione-s-transferase was identified and characterized in terms of their isozymic forms, tyrosine phosphorylation, and immunolocalization patterns in cryopreserved buffalo spermatozoa in comparison w ... | 2014 | 24388131 |
| two different macaviruses, ovine herpesvirus-2 and caprine herpesvirus-2, behave differently in water buffaloes than in cattle or in their respective reservoir species. | the ongoing global spread of "exotic" farm animals, such as water buffaloes, which carry their native sets of viruses, may bear unknown risks for the animals, into whose ecological niches the former are introduced and vice versa. here, we report on the occurrence of malignant catarrhal fever (mcf) on swiss farms, where "exotic" water buffaloes were kept together with "native" animals, i.e. cattle, sheep, and goats. in the first farm with 56 water buffaloes, eight cases of mcf due to ovine herpes ... | 2013 | 24386255 |
| evidence for bovine besnoitiosis in egypt-first serosurvey of besnoitia besnoiti in cattle and water buffalo (bubalus bubalis) in egypt. | the present study evaluated the presence of specific antibodies against besnoitia besnoiti in cattle and water buffalo (bubalus bubalis) in egypt. sera from cattle (n = 216) and water buffaloes (n = 133) collected from five different provinces of egypt (behera, alexandria, assuit, gharbia, and matrouh) were analyzed. testing for b. besnoiti antibodies by priocheck® besnoitia ab 2.0 elisa initially identified 13.75 % (48 out of 349) of individual sera as positive at the manufacturer's suggested c ... | 2014 | 24375275 |
| comparative fish mapping of bmpr1b, bmp15 and gdf9 fecundity genes on cattle, river buffalo, sheep and goat chromosomes. | 2013 | 24371182 | |
| genetic characterization of babesia and theileria parasites in water buffaloes in sri lanka. | water buffaloes are thought to be the reservoir hosts for several hemoprotozoan parasites that infect cattle. in the present study, we surveyed sri lankan bred water buffaloes for infections with babesia bovis, babesia bigemina, theileria annulata, and theileria orientalis using parasite-specific pcr assays. when 320 blood-derived dna samples from water buffaloes reared in three different districts (polonnaruwa, mannar, and mullaitivu) of sri lanka were pcr screened, b. bovis, b. bigemina, and t ... | 2014 | 24365246 |
| peripheral blood mononuclear cells: a potential cellular system to understand differential heat shock response across native cattle (bos indicus), exotic cattle (bos taurus), and riverine buffaloes (bubalus bubalis) of india. | circulating leukocytes can be used as an effective model to understand the heat stress response of different cattle types and buffaloes. this investigation aimed to determine the temporal profile of hsps (hsp40, hsp60, hsp70, and hsp90) expression in circulating peripheral blood mononuclear cells (pbmcs) of murrah buffaloes, holstein-friesian (hf), and sahiwal cows in response to sublethal heat shock at 42 °c. the viability data indicated hf pbmcs to be the most affected to the heat shock, where ... | 2013 | 24363171 |
| tuberculosis prevalence and risk factors for water buffalo in pará, brazil. | the prevalence of and possible risk factors for tuberculosis were studied in water buffalo from pará, brazil. in this study, 3,917 pregnant and nonpregnant female murrah and mediterranean buffaloes were studied; 2,089 originated from marajó island, and 1,108 were from the mainland. the comparative cervical tuberculin test was used as a diagnostic test for tuberculosis in these animals. the prevalence of positive buffaloes was 3.5 % (100/2,809) on marajó island and 7.2 % (80/1,108) on the mainlan ... | 2014 | 24356890 |
| characterisation and in silico analysis of interleukin-4 cdna of nilgai (boselaphus tragocamelus) and indian buffalo (bubalus bubalis). | interleukin-4 (il-4) produced from th2 cells modulates both innate and adaptive immune responses. it is a common belief that wild animals possess better immunity against diseases than domestic and laboratory animals; however, the immune system of wild animals is not fully explored yet. therefore, a comparative study was designed to explore the wildlife immunity through characterisation of il-4 cdna of nilgai, a wild ruminant, and indian buffalo, a domestic ruminant. total rna was extracted from ... | 2013 | 24348167 |
| prevalence of subclinical coccidiosis in river buffalo calves of southwest of iran. | despite the importance of buffalo farming in iran, little is known in this country about the abundance and distribution of eimeria spp. in the animal species. the present study was designed to investigate the prevalence and species characterization of eimeria oocysts in river buffalo calves of khuzestan province, southwest of iran. of the total 108 fecal samples examined for eimeria, 108 (100%) were found infected with 11 species of the parasite. among the identified species of eimeria, e. bovis ... | 2013 | 24338314 |
| bacterial diversity associated with feeding dry forage at different dietary concentrations in the rumen contents of mehshana buffalo (bubalus bubalis) using 16s pyrotags. | pyrosequencing of 16s rrna gene targeting bacteria was applied to identify diet-induced shifts in the microbiome of both solid and liquid ruminal fractions retrieved from water buffalo fed different diets. the depth of coverage of metabolically active bacteria in a community using different primer pairs was also investigated. to assess reproducibility, animal to animal variation was considered in all phylogenetic and community comparisons. the experiment included four non-lactating water buffalo ... | 2014 | 24315806 |
| comparative sequence analysis in the exon 5 of growth hormone gene in the various livestock species of india. | the aim of the study was to identify genetic polymorphism in growth hormone (gh) gene locus of six different livestock species using pcr-direct dna sequencing method. in exon 5 of gh gene, 10 snps variants were identified in all livestock species studied, namely bubalus bubalis, bos indicus, bos frontalis, bos grunniens, ovis aries, and capra hircus. four snps were observed in bubalus bubalis, two snps in bos indicus, one snp in ovis aries, and three snps in capra hircus. no changes were observe ... | 2014 | 24299185 |
| cloning, e. coli expression, and characterization of heart lactate dehydrogenase b from river buffalo (bubalus bubalis). | lactate dehydrogenase is an enzyme of glycolytic pathway which catalyzes the interconversion of pyruvate and lactate. the present study describes cdna cloning, e. coli expression and characterization of lactate dehydrogenase b (ldh-b) from the heart ventricles of river buffalo (bubalus bubalis). total rna was isolated from the heart tissue, a 1005bp cdna encoding complete polypeptide chain of 334 amino acids was generated by reverse transcriptase reaction and analyzed for nucleotide sequence. th ... | 2014 | 24299182 |
| developmental competence of buffalo (bubalus bubalis) pluripotent embryonic stem cells over different homologous feeder layers and the comparative evaluation with various extracellular matrices. | use of somatic cells as a feeder layer to maintain the embryonic stem cells (escs) in undifferentiated state limits the stem cell research design, since experimental data may result from a combined escs and feeder cell response to various stimuli. therefore, present study was designed to evaluate the developmental competence of the buffalo escs over different homogenous feeders and compare with various extracellular matrices using different concentrations of lif. | 2013 | 24298371 |
| effect of flubendiamide, lead and their combined exposure on erythrocytic indices in water buffalo calves. | this study evaluated the effect of flubendiamide, lead and their combined oral administration on erythrocytic indices in water buffalo calves. exposure to flubendiamide alone resulted in non-significant decreases in hemoglobin (hb), packed cell volume (pcv), total erythrocyte count (tec), mean corpuscular volume (mcv) and mean corpuscular hemoglobin (mch). lead acetate exposure resulted in significant declines in hb, pcv and tec. combined exposure to flubendiamide and lead resulted in declines i ... | 2014 | 24292848 |
| evaluation of hemostatic activity of latex from three euphorbiaceae species. | latices from several plant species of euphorbiaceae family have been traditionally applied over fresh cuts to stop bleeding and subsequently applied over wounds to enhance healing process. the latex arrested bleeding from fresh wounds by reducing bleeding and whole blood coagulation time which are important indices of hemostatic activity. it has been accepted that hemostatic activity is due to the proteolytic fraction of plant latices. thus, the present study aimed to assess the clot inducing pr ... | 2014 | 24291032 |
| expression and localization of locally produced growth factors regulating lymphangiogenesis during different stages of the estrous cycle in corpus luteum of buffalo (bubalus bubalis). | recent experiments using expression, immunolocalization, and cell culture approaches have provided leading insights into regulation of luteal angiogenesis by different growth factor systems and its role in the function of corpus luteum (cl) in buffalo. on the contrary, lymphangiogenesis and its regulation in the cl are still poorly understood. the aim of this study was to evaluate the expression and localization of lymphangiogenic factors (vascular endothelial growth factor [vegf]-c and vegfd), ... | 2014 | 24246422 |
| cryogenic changes in proteases and antiprotease activities of buffalo (bubalus bubalis) and cattle (bos taurus) semen. | the postthaw motility and fertility of buffalo and cattle semen is reduced when they are cryopreserved for artificial insemination. in the present study, an attempt was made to characterize the cryogenic changes in proteases and antiprotease activities (apa) of buffalo and cattle semen because these proteolysis regulators have been reported to be associated with sperm motility and fertility. buffalo sperm demonstrated at least two major proteases of 45 and 42 kda and three minor proteases of 95, ... | 2014 | 24210916 |
| identification of virulence factors in 16s-23s rrna intergenic spacer genotyped staphylococcus aureus isolated from water buffaloes and small ruminants. | staphylococcus aureus is an important human and animal pathogen, and is regarded as an important cause of intramammary infection (imi) in ruminants. staphylococcus aureus genetic variability and virulence factors have been well studied in veterinary medicine, especially in cows as support for control and management of imi. the aim of the present study was to genotype 71 staph. aureus isolates from the bulk tank and foremilk of water buffaloes (n=40) and from udder tissue (n=7) and foremilk (n=24 ... | 2013 | 24140323 |
| endocrine changes, timing of ovulation, ovarian follicular growth and efficacy of a novel protocol (estradoublesynch) for synchronization of ovulation and timed artificial insemination in murrah buffaloes (bubalus bubalis). | experiments were conducted to investigate (1) ovarian follicular growth, timing of ovulation and associated endocrine changes (progesterone, estrogen, and lh) in cycling, and (2) efficacy in terms of pregnancy rate in cycling and anestrus murrah buffaloes subjected to the estradoublesynch protocol (prostaglandin f2α [pgf2α] 0, gnrh 2, pgf2α 9, estradiol benzoate, eb 10). twelve cycling buffaloes were subjected to the estradoublesynch protocol and observed for ovulation, follicle size, and endocr ... | 2014 | 24125725 |
| molecular cloning, sequence characterization, and gene expression profiling of a novel water buffalo (bubalus bubalis) gene, agpat6. | several 1-acylglycerol-3-phosphate-o-acyltransferases (agpats) can acylate lysophosphatidic acid to produce phosphatidic acid. of the eight agpat isoforms, agpat6 is a crucial enzyme for glycerolipids and triacylglycerol biosynthesis in some mammalian tissues. we amplified and identified the complete coding sequence (cds) of the water buffalo agpat6 gene by using the reverse transcription-polymerase chain reaction, based on the conversed sequence information of the cattle or expressed sequence t ... | 2013 | 24114207 |
| arachidic acid in extender improves post-thaw parameters of cryopreserved nili-ravi buffalo bull semen. | cryopreservation process reduces lipids and phospholipids from buffalo bull spermatozoa. it was therefore hypothesized that supplementation of fatty acid to extender may improve the post-thaw quality of buffalo semen. the objective was to evaluate the effect of arachidic acid supplementation in extender on post-thaw quality of buffalo bull (bubalus bubalis) spermatozoa. semen was collected from three adult nili-ravi buffalo bulls of similar age group with artificial vagina (42°c) for 3 weeks (re ... | 2014 | 24112366 |
| local immune responses of the chinese water buffalo, bubalus bubalis, against schistosoma japonicum larvae: crucial insights for vaccine design. | asian schistosomiasis is a zoonotic parasitic disease infecting up to a million people and threatening tens of millions more. control of this disease is hindered by the animal reservoirs of the parasite, in particular the water buffalo (bubalus bubalis), which is responsible for significant levels of human transmission. a transmission-blocking vaccine administered to buffaloes is a realistic option which would aid in the control of schistosomiasis. this will however require a better understandin ... | 2013 | 24086786 |
| the mammary gland in domestic ruminants: a systems biology perspective. | milk and dairy products are central elements in the human diet. it is estimated that 108kg of milk per year are consumed per person worldwide. therefore, dairy production represents a relevant fraction of the economies of many countries, being cattle, sheep, goat, water buffalo, and other ruminants the main species used worldwide. an adequate management of dairy farming cannot be achieved without the knowledge on the biological mechanisms behind lactation in ruminants. thus, understanding the mo ... | 2013 | 24076120 |
| use of real-time pcr technique in determination of major fibrolytic and non fibrolytic bacteria present in indian surti buffaloes (bubalus bubalis). | in the milk industry in india, buffalo breeds are most commonly used for milk production. efficiency of fiber digestion in ruminants is critical for animal productivity. bacteria play an important role in fiber digestion and utilization. absolute quantification real-time pcr was used to quantify ten bacterial species in rumen fluid of surti buffalo fed green fodder, dry roughage and compound concentrate mixture. abundance of each target taxon was calculated as a fraction of the total 16s rrna ge ... | 2013 | 24053023 |
| carboxypeptidase-b from bubalus bubalis pancreas: purification, properties and maldi-tof monitored activation of proinsulin. | carboxypeptidase-b (e.c 3.4.17.2) catalyzes the hydrolysis of peptides and esters at c-terminus of arginine and lysine residues. our study describes the large scale purification, n-terminal sequence analysis and physiochemical properties of pancreatic enzyme from river buffalo (bubalus bubalis). the enzyme was purified up to 71 folds by anion-exchange chromatography with 21% final recovery. purified enzyme displayed two bands on sds-page with molecular weights of 9 kda and 26 kda respectively, t ... | 2013 | 24035945 |
| molecular identification of methanogenic archaea from surti buffaloes (bubalus bubalis), reveals more hydrogenotrophic methanogens phylotypes. | methane emissions from ruminant livestock are considered to be one of the more potent forms of greenhouses gases contributing to global warming. many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an approach can only be successful if it targets all the major groups of ruminant methanogens. therefore, a thorough knowledge of the diversity of these microbes in breeds of buffaloes, as well as in response to geographical location and different diets, i ... | 2011 | 24031614 |
| comparative evaluation of rumen metagenome community using qpcr and mg-rast. | microbial profiling of metagenome communities have been studied extensively using mg-rast and other related metagenome annotation databases. although, database based taxonomic profiling provides snapshots of the metagenome architecture, their reliability needs to be validated through more accurate methods. here, we performed qpcr based absolute quantitation of selected rumen microbes in the liquid and solid fraction of the rumen fluid of river buffalo adapted to varying proportion of concentrate ... | 2013 | 24025701 |
| isolation and characterization of luteal cells in buffalo (bubalus bubalis). | the objective of the study was to evaluate the morphological and functional characteristics of luteal cells isolated from buffalo ovary. luteal cells exhibited columnar morphology and contact inhibition at the stage of confluence. protein concentrations increased linearly from day 3 to day 7 of culture. dna concentrations increased from day 3 to day 5 and then declined to day 7 of culture. pgf2alpha concentrations decreased progressively from day 3 to day 7 of culture. it was concluded that buff ... | 2013 | 24020092 |
| effect of feeding inorganic chromium on growth performance, endocrine variables, and energy metabolites in winter-exposed buffalo calves (bubalus bubalis). | we investigated the effect of chromium (cr) supplementation on the growth performance, energy metabolites, and hormonal variation in winter-exposed buffalo calves. twenty-four female buffalo calves were randomly allotted to four dietary treatments (n = 6) for a period of 120 days. feeding regimen was the same in all the groups, except the animals in the four respective groups were additionally supplemented with 0.0, 0.5, 1.0, and 1.5 mg of cr/kg dm in the form of crcl3.6h2o. calves were monitore ... | 2013 | 24013931 |
| treatment of donor cells with trichostatin a improves in vitro development and reprogramming of buffalo (bubalus bubalis) nucleus transfer embryos. | it has been reported that buffalo (bubalus bubalis) embryos reconstructed by somatic cell nucleus transfer (scnt) can develop to the full term of gestation and result in newborn calves. however, the developmental competence of reconstructed embryos is still low. recently, it has been reported that treating donor cells or embryos with trichostatin a (tsa) can increase the cloning efficiency in some species. thus, the present study was undertaken to improve the development of buffalo scnt embryos ... | 2013 | 24007823 |
| expression pattern of glucose metabolism genes in relation to development rate of buffalo (bubalus bubalis) oocytes and in vitro-produced embryos. | the expression pattern of glucose metabolism genes (hexokinase, phosphofructokinase, glucose-6-phosphate dehydrogenase [g6pdh], lactate dehydrogenase [ldh], and pyruvate dehydrogenase [pdh]) were studied in buffalo in vitro-matured oocytes and in vitro-produced embryos cultured under different glucose concentrations (0 mm, 1.5 mm, 5.6 mm, and 10 mm) during in vitro maturation of oocytes and culture of ivf produced embryos. the expression of the genes varied significantly over the cleavage stages ... | 2013 | 23992643 |
| establishment and biological characteristics comparison of chinese swamp buffalo (bubalus bubalis) fibroblast cell lines. | to establish fibroblast cell lines from different tissues and to compare the biological characteristics of those cell lines, five fibroblast cell lines derived from chinese swamp buffalo (bubalus bubalis) were selected for comparative assays. cell style and survival rate (before cryogenic preservation and after recovery) were tested, and karyotype, patterns of isoenzymes of lactic dehydrogenase, malic dehydrogenase, and cell cycle were analyzed. these cell lines had a healthy morphology with a t ... | 2014 | 23990385 |
| oxytocin induced oxidative stress in lactating bubalis bubalis (nili ravi). | oxytocin has been reported for a wide range of adverse effects in different species of lactating animals. the present study was aimed to evaluate the adverse effects of oxytocin on biomarkers of oxidative stress in buffaloes. lactating buffaloes (n = 40) were randomly selected from a commercial dairy farm located in the peri-urban area of district faisalabad, pakistan and divided into two equal groups viz. treatment and control groups, each containing 20 buffaloes. buffaloes in treatment group w ... | 2013 | 23981231 |
| assessment of chromium content of feedstuffs, their estimated requirement, and effects of dietary chromium supplementation on nutrient utilization, growth performance, and mineral balance in summer-exposed buffalo calves (bubalus bubalis). | this study was conducted to determine the chromium content of different feedstuffs, their estimated requirement, and effect of dietary cr supplementation on nutrient intake, nutrient utilization, growth performance, and mineral balance in buffalo calves during summer season. levels of cr was higher in cultivated fodder, moderate in cakes and cereal grains, while straw, grasses, and non-conventional feeds were poor in cr content. to test the effect of cr supplementation in buffalo calves, 0, 0.5, ... | 2013 | 23963742 |
| malignant catarrhal fever: an emerging disease in the african buffalo (syncerus caffer). | within the tribe bovini in the subfamily bovinae, the water buffalo (bubalus bubalis), american bison (bison bison), european bison (bubalus bonasus) and yak (bos grunniens) are recognized as species highly susceptible to malignant catarrhal fever (mcf). in contrast, the lack of reports describing clinical mcf in the african buffalo (syncerus caffer) whether free ranging or captive has led to a perception that african buffaloes are resistant to mcf. during the last decade, several cases of mcf i ... | 2015 | 23957274 |
| microarray analysis of gene expression profiles of schistosoma japonicum derived from less-susceptible host water buffalo and susceptible host goat. | water buffalo and goats are natural hosts for s. japonicum in endemic areas of china. the susceptibility of these two hosts to schistosome infection is different, as water buffalo are less conducive to s. japonicum growth and development. to identify genes that may affect schistosome development and survival, we compared gene expression profiles of schistosomes derived from these two natural hosts using high-throughput microarray technology. | 2013 | 23940568 |
| cellular and functional characterization of buffalo (bubalus bubalis) corpus luteum during the estrous cycle and pregnancy. | in the present paper, cellular composition of buffalo corpus luteum (cl) with its functional characterization based on 3β-hsd and progesterone secretory ability at different stages of estrous cycle and pregnancy was studied. buffalo uteri along with ovaries bearing cl were collected from the local slaughter house. these were classified into different stages of estrous cycle (stage i, ii, iii and iv) and pregnancy (stage i, ii and iii) based on morphological appearance of cl, surface follicles on ... | 2013 | 23896394 |
| first molecular characterisation of cryptosporidium and giardia from bubalus bubalis (water buffalo) in victoria, australia. | we conducted a molecular epidemiological survey of cryptosporidium and giardia from bubalus bubalis (water buffalo) on two extensive farms (450 km apart) in victoria, australia. faecal samples (n=476) were collected from different age groups of water buffalo at two time points (six months apart) and tested using a pcr-based mutation scanning-targeted sequencing-phylogenetic approach, employing markers within the small subunit of ribosomal rna (designated pssu) and triose phosphate isomerase (ptp ... | 2013 | 23886616 |
| gas chromatographic-mass spectrometric analysis of chemical volatiles in buffalo (bubalus bubalis) urine. | isolation of active fraction and characterization of chemosignals from urine have been attempted in several mammalian species in the recent years. the objective of this study was to identify the urinary volatiles across various reproductive stages of buffalo cow, namely, estrus, diestrus, and pregnancy, and in bull, by chemical extraction followed by gas chromatography-linked mass spectrometry (gc-ms). urine samples were collected from six buffalo cows at two different phases of estrous cycle, n ... | 2013 | 23876684 |
| myoglobin as marker in meat adulteration: a uplc method for determining the presence of pork meat in raw beef burger. | the identification of meat animal species used in raw burgers is very important with respect to economic and religious considerations. therefore, international supervisory bodies have implemented procedures to control the employed meat species. in this paper we propose myoglobin as a powerful molecular marker to evaluate the presence of non-declared meat addition in raw beef burgers by using ultra-performance liquid chromatography (uplc) for the separation and identification of edible animal spe ... | 2013 | 23870895 |
| molecular genetic diversity and quantitation of methanogen in ruminal fluid of buffalo (bubalus bubalis) fed ration (wheat straw and concentrate mixture diet). | high roughage diet causes more methane emissions; however, the total methanogen abundance is not influenced by roughage proportion. technologies to reduce methane emissions are lacking, and development of inhibitors and vaccines that mitigate rumen-derived methane by targeting methanogens relies on present knowledge of the methanogens. in this work, we have investigated molecular diversity of rumen methanogens of surti buffalo. dna from rumen fluid was extracted, and 16s rrna encoding genes were ... | 2013 | 23862067 |
| novel polymorphisms of the igf1r gene and their association with average daily gain in egyptian buffalo (bubalus bubalis). | the objective of this study was to detect insulin-like growth factor 1 receptor (igf1r) polymorphisms, their allele, and genotype frequencies and to determine associations between these polymorphisms and growth traits in egyptian water buffalo. three loci of the igf1r coding region were amplified by rt-pcr and, subsequently, subjected to sequence analysis, followed by single-strand conformation polymorphism to identify different allelic patterns. a total of 11 novel polymorphisms were detected; ... | 2013 | 23820243 |
| homology modeling and structural comparison of leucine rich repeats of toll like receptors 1-10 of ruminants. | toll-like receptors (tlrs) are transmembrane receptors composed of extra cellular leucine rich repeats (lrrs) that identify specific pathogen associated molecular patterns triggering a innate immune cascade. the lrr regions of tlr 1-10 proteins of goat (capra hircus), sheep (ovis aries), buffalo (bubalus bubalis) and bovine (bos taurus) were modeled using modeller 9v7 tool and validated. the similarities and variations of these 10 tlrs extracellular regions of each species were compared using on ... | 2013 | 23812948 |
| survival of arcobacter butzleri during production and storage of artisan water buffalo mozzarella cheese. | water buffalo mozzarella cheese (wbmc) is a fresh stretched cheese produced from whole chilled buffalo milk. although pasteurization of milk and the use of defined starter cultures are recommended, traditional technology involving unpasteurized milk and natural whey cultures is still employed for wbmc production in italy. the purpose of this study was to assess the behavior of arcobacter butzleri during wbmc production and storage under different temperature conditions (5, 10, and 20 °c). raw mi ... | 2013 | 23806147 |
| the behaviour and welfare of buffaloes (bubalus bubalis) in modern dairy enterprises. | this review deals with the behaviour of river buffaloes (bubalus bubalis), in confinement and in extensive conditions, also focusing on the effects of different housing and rearing conditions on their welfare. the behavioural repertoire expressed by buffaloes in extensive and intensive conditions is similar to those displayed by other domestic ruminants. however, through natural selection, buffaloes have also acquired several morphological, physiological and behavioural (i.e. wallowing) adaptati ... | 2013 | 23803231 |
| molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (gm-csf) from indian water buffalo (bubalus bubalis). | granulocyte-macrophage colony-stimulating factor (gm-csf) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. in this study, we report molecular cloning, sequencing and characterization of gm-csf from indian water buffalo, bubalus bubalis. in addition, we performed sequence and structural analysis for buffalo gm-csf. buffalo gm-csf has been compared with 17 mammalian gm-csfs using multiple sequence alignment and phylogenetic tree. ... | 2014 | 23800159 |