Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| spatial analysis of melioidosis distribution in a suburban area. | burkholderia pseudomallei, the causative agent of melioidosis is associated with soil. this study used a geographic information system (gis) to determine the spatial distribution of clinical cases of melioidosis in the endemic suburban region of townsville in australia. a total of 65 cases over the period 1996-2008 were plotted using residential address. two distinct groupings were found. one was around the base of a hill in the city centre and the other followed the old course of a major waterw ... | 2010 | 20092666 |
| screening a mushroom extract library for activity against acinetobacter baumannii and burkholderia cepacia and the identification of a compound with anti-burkholderia activity. | acinetobacter baumannii and species within the burkholderia cepacia complex (bcc) are significant opportunistic bacterial pathogens of humans. these species exhibit a high degree of antibiotic resistance, and some clinical isolates are resistant to all currently available antimicrobial drugs used for treatment. thus, new drugs are needed to treat infections by these species. mushrooms could be a potential source for new drugs to treat a. baumannii and bcc infections. | 2010 | 20092635 |
| identification of potential therapeutic targets for burkholderia cenocepacia by comparative transcriptomics. | burkholderia cenocepacia is an endemic soil dweller and emerging opportunistic pathogen in patients with cystic fibrosis (cf). the identification of virulence factors and potential therapeutic targets has been hampered by the genomic diversity within the species as many factors are not shared among the pathogenic members of the species. | 2010 | 20090946 |
| within-host evolution of burkholderia pseudomallei in four cases of acute melioidosis. | little is currently known about bacterial pathogen evolution and adaptation within the host during acute infection. previous studies of burkholderia pseudomallei, the etiologic agent of melioidosis, have shown that this opportunistic pathogen mutates rapidly both in vitro and in vivo at tandemly repeated loci, making this organism a relevant model for studying short-term evolution. in the current study, b. pseudomallei isolates cultured from multiple body sites from four thai patients with disse ... | 2010 | 20090837 |
| sequence polymorphism and pcr-restriction fragment length polymorphism analysis of the flagellin gene of burkholderia pseudomallei. | four flagellin allelic types (i to iv) of burkholderia pseudomallei were identified based on their sequence variation and restriction fragment length polymorphism (rflp) analysis of the amplified flagellin gene. flagellin allelic type i was the most predominantly (75.0%) found among the 100 clinical isolates of b. pseudomallei investigated in this study. | 2010 | 20089759 |
| production and characterization of biosurfactants produced by microorganisms isolated from milk factory wastewaters. | biosurfactants or surface-active compounds are produced by microorganisms. these molecules reduce the surface tension of both aqueous solutions and hydrocarbon mixtures. in this study, the isolation and identification of biosurfactant-producing microorganisms were assessed. the characterization of biosurfactant produced by microorganisms isolated from milk factory wastewaters was investigated. for this purpose, five different microorganisms were isolated and identified. in order to determine the ... | 2009 | 20088204 |
| burkholderia cenocepacia creates an intramacrophage replication niche in zebrafish embryos, followed by bacterial dissemination and establishment of systemic infection. | bacteria belonging to the "burkholderia cepacia complex" (bcc) often cause fatal pulmonary infections in cystic fibrosis patients, yet little is know about the underlying molecular mechanisms. these gram-negative bacteria can adopt an intracellular lifestyle, although their ability to replicate intracellularly has been difficult to demonstrate. here we show that bcc bacteria survive and multiply in macrophages of zebrafish embryos. local dissemination by nonlytic release from infected cells was ... | 2010 | 20086083 |
| characterization of hydrogen peroxide production by duox in bronchial epithelial cells exposed to pseudomonas aeruginosa. | hydrogen peroxide production by the nadph oxidase duox1 occurs during activation of respiratory epithelial cells stimulated by purified bacterial ligands, such as lipopolysaccharide. here, we characterize duox activation using intact bacterial cells of several airway pathogens. we found that only pseudomonas aeruginosa, not burkholderia cepacia or staphylococcus aureus, triggers h2o2 production in bronchial epithelial cells in a calcium-dependent but predominantly atp-independent manner. moreove ... | 2010 | 20085766 |
| burkholdines 1097 and 1229, potent antifungal peptides from burkholderia ambifaria 2.2n. | potent antifungal cyclic lipopeptides, burkholdines (bk), were isolated from a culture of burkholderia ambifaria 2.2n. bk-1229 (1) and bk-1097 (2) are octapeptides comprised of nonproteinogenic amino acids, including beta-hydroxytyrosine, beta-hydroxyasparagine, and a new fatty acyl amino acid. 1 and 2 are fungicidal against a panel of fungi with potencies 2-60-fold better than amphotericin b control. | 2010 | 20085289 |
| potential mechanisms underlying the acute lung dysfunction and bacterial extrapulmonary dissemination during burkholderia cenocepacia respiratory infection. | burkholderia cenocepacia, an opportunistic pathogen that causes lung infections in cystic fibrosis (cf) patients, is associated with rapid and usually fatal lung deterioration due to necrotizing pneumonia and sepsis, a condition known as cepacia syndrome. the key bacterial determinants associated with this poor clinical outcome in cf patients are not clear. in this study, the cytotoxicity and procoagulant activity of b. cenocepacia from the et-12 lineage, that has been linked to the cepacia synd ... | 2010 | 20082687 |
| expanding small-molecule functional metagenomics through parallel screening of broad-host-range cosmid environmental dna libraries in diverse proteobacteria. | the small-molecule biosynthetic diversity encoded within the genomes of uncultured bacteria is an attractive target for the discovery of natural products using functional metagenomics. phenotypes commonly associated with the production of small molecules, such as antibiosis, altered pigmentation, or altered colony morphology, are easily identified from screens of arrayed metagenomic library clones. however, functional metagenomic screening methods are limited by their intrinsic dependence on a h ... | 2010 | 20081001 |
| changing spectrum of microbiology of liver abscess: now klebsiella, next burkholderia pseudomallei. | 2010 | 20079999 | |
| application of multiplex single nucleotide primer extension (msnupe) to the identification of bacteria: the burkholderia cepacia complex case. | burkholderia cepacia complex (bcc) is characterized by a complex taxonomy constituted by seventeen closely related species of both biotechnological and clinical importance. several molecular methods have been developed to accurately identify bcc species but simpler and effective strategies for bcc classification are still needed. a single nucleotide primer extension (snupe) assay using gyrb as a target gene was developed to identify bacteria belonging to the b. cepacia (bcc) complex. this techni ... | 2010 | 20079386 |
| insights into the history of the legume-betaproteobacterial symbiosis. | the interaction between legumes and rhizobia has been well studied in the context of a mutualistic, nitrogen-fixing symbiosis. the fitness of legumes, including important agricultural crops, is enhanced by the plants' ability to develop symbiotic associations with certain soil bacteria that fix atmospheric nitrogen into a utilizable form, namely, ammonia, via a chemical reaction that only bacteria and archaea can perform. of the bacteria, members of the alpha subclass of the protebacteria are th ... | 2010 | 20078769 |
| subdivision of the bacterioferritin comigratory protein family of bacterial peroxiredoxins based on catalytic activity. | peroxiredoxins are ubiquitous proteins that catalyze the reduction of hydroperoxides, thus conferring resistance to oxidative stress. using high-resolution mass spectrometry, we recently reclassified one such peroxiredoxin, bacterioferritin comigratory protein (bcp) of escherichia coli, as an atypical 2-cys peroxiredoxin that functions through the formation of an intramolecular disulfide bond between the active and resolving cysteine. an engineered e. coli bcp, which lacked the resolving cystein ... | 2010 | 20078128 |
| genetic and phenotypic diversity of parathion-degrading bacteria isolated from rice paddy soils. | three parathion-degrading bacteria and eight pairs of bacteria showing syntrophic metabolism of parathion were isolated from rice field soils, and their genetic and phenotypic characteristics were investigated. the three isolates and eight syntrophic pairs were able to utilize parathion as a sole source of carbon and energy, producing p-nitrophenol as the intermediate metabolite during the complete degradation of parathion. analysis of 16s rrna gene sequence indicated that the isolates were rela ... | 2009 | 20075637 |
| population pharmacokinetic comparison and pharmacodynamic breakpoints of ceftazidime in cystic fibrosis patients and healthy volunteers. | despite the promising activity of ceftazidime against pseudomonas aeruginosa and burkholderia cepacia, there has not yet been a study that directly compared the pharmacokinetics (pk) of ceftazidime in cystic fibrosis (cf) patients and healthy volunteers by population pk methodology. we assessed the population pk and pk/pharmacodynamic (pd) breakpoints of ceftazidime in cf patients and healthy volunteers. eight cf patients (total body weight [wt] [average +/- standard deviation] = 42.9 +/- 18.4 k ... | 2010 | 20065059 |
| the survival of burkholderia pseudomallei in liquid media. | we studied the effect of environmental parameters on the survival of burkholderia pseudomallei. there was a small increase in bacterial count for up to 28 days in sterilized distilled water or rain water, in water at 20 degrees c or 40 degrees c, and in buffered solutions of ph 4 or higher. counts of culturable b. pseudomallei declined at ph 3, in the presence of seawater or water with concentrations of 4% salt or higher, and under refrigeration. the morphological appearances of b. pseudomallei ... | 2010 | 20065001 |
| human pathogens abundant in the bacterial metagenome of cigarettes. | many studies have evaluated chemical, heavy metal, and other abiotic substances present in cigarettes and their roles in the development of lung cancer and other diseases, yet no studies have comprehensively evaluated bacterial diversity of cigarettes and the possible impacts of these microbes on respiratory illnesses in smokers and exposed nonsmokers. | 2010 | 20064769 |
| insights into the biosynthesis and stability of the lasso peptide capistruin. | capistruin is a 19-residue ribosomally synthesized lasso peptide encoded by the capabcd gene cluster in burkholderia thailandensis. it is composed of an n-terminal 9-residue macrolactam ring, through which the 10-residue c-terminal tail is threaded. using a heterologous capistruin production system in escherichia coli, we have generated 48 mutants of the precursor protein capa to gain insights into capistruin biosynthesis. only 4 residues (gly1, arg11, val12, and ile13) of the lasso sequence wer ... | 2009 | 20064439 |
| determination of diketopiperazines of burkholderia cepacia cf-66 by gas chromatography-mass spectrometry. | bacteria communicate with each other by a process termed "quorum sensing" (qs), and diffusible, low-molecular-weight chemicals, called signal molecules, are used as the communication languages. in cell-free burkholderia cepacia cf-66 culture supernatants, five compounds suspected of being signal molecules were identified. the gene (cepi) related with ahls synthesis were not detected by polymerase chain reaction (pcr) using specific primers. gas chromatography-mass spectrometry (gc-ms) revealed t ... | 2010 | 20062980 |
| mechanism and inhibition of the fabv enoyl-acp reductase from burkholderia mallei. | enoyl-acp reductases catalyze the final step in the elongation cycle of the bacterial fatty acid biosynthesis (fas-ii) pathway. at present, four distinct enoyl-acp reductases have been identified, which are the products of the fabi, fabl, fabk, and fabv genes. the fabv enoyl-acp reductase is the most recent member of this enzyme class and was originally identified in vibrio cholerae by cronan and co-workers [massengo-tiasse, r. p., and cronan, j. e. (2008) vibrio cholerae fabv defines a new clas ... | 2010 | 20055482 |
| expression, purification, crystallization and preliminary x-ray analysis of maleylacetate reductase from burkholderia sp. strain sj98. | maleylacetate reductase (ec 1.3.1.32) is an important enzyme that is involved in the degradation pathway of aromatic compounds and catalyzes the reduction of maleylacetate to 3-oxoadipate. the gene pnpd encoding maleylacetate reductase in burkholderia sp. strain sj98 was cloned, expressed in escherichia coli and purified by affinity chromatography. the enzyme was crystallized in both native and semet-derivative forms by the sitting-drop vapour-diffusion method using peg 3350 as a precipitant at ... | 2009 | 20054138 |
| pseudomonas aeruginosa alginate promotes burkholderia cenocepacia persistence in cystic fibrosis transmembrane conductance regulator knockout mice. | pseudomonas aeruginosa, a major respiratory pathogen in cystic fibrosis (cf) patients, facilitates infection by other opportunistic pathogens. burkholderia cenocepacia, which normally infects adolescent patients, encounters alginate elaborated by mucoid p. aeruginosa. to determine whether p. aeruginosa alginate facilitates b. cenocepacia infection in mice, cystic fibrosis transmembrane conductance regulator knockout mice were infected with b. cenocepacia strain bc7 suspended in either phosphate- ... | 2010 | 20048042 |
| critical protective role for mcp-1 in pneumonic burkholderia mallei infection. | burkholderia mallei is a gram-negative bacterial pathogen of domestic equidae and humans that can cause severe, rapidly life-threatening pneumonic infections. little is known regarding the role of chemokines and early cellular immune responses in protective immunity to pulmonary infection with b. mallei. although the role of mcp-1 in gram-positive bacterial infections has been previously investigated, the role of mcp-1 in immunity to acute pneumonia caused by gram-negative bacteria, such as b. m ... | 2010 | 20042590 |
| growth-related changes in intracellular spermidine and its effect on efflux pump expression and quorum sensing in burkholderia pseudomallei. | the burkholderia pseudomallei bpeab-oprb resistance-nodulation-division (rnd) family pump effluxes aminoglycoside and macrolide antibiotics as well as acylhomoserine lactones (ahls) involved in quorum sensing. expression of bpea-lacz was cell density-dependent and was inducible in the presence of these compounds. intracellular levels of spermidine and n-acetylspermidine increased with cell density in wild-type b. pseudomallei khw, but were always lower in the bpeab pump mutant at all growth phas ... | 2010 | 20035006 |
| homologous overexpression of a lipase from burkholderia cepacia using the lambda red recombinase system. | red recombinase system of the lambda phage is widely used for recombination of short linear dna fragments and genome. using this system, we obtained t7 rna polymerase (rnap) substitution mutants in burkholderia cepacia. to test the expression abilities of the t7 mutants, four different lipase expression vectors were transformed and the lipase activity of these recombinants was evaluated. our results suggest that 500 nt homology between the unit and the genome is sufficient to generate mutations ... | 2010 | 20033831 |
| [melioidosis: an emerging tropical disease]. | melioidosis is an infection affecting both human and animal health. the causative agent is burkholderia pseudomallei, a gram-negative soil bacterium. melioidosis is endemic in tropical areas of southeast asia and northern australia, and sporadic in many other countries. clinical presentation is variable ranging from acute septicemia, isolated pulmonary infection, or chronic granulomatous lesions to asymptomatic forms with positive serology. there is no vaccine and treatment is difficult because ... | 2009 | 20025169 |
| rapid solubilization of insoluble phosphate by a novel environmental stress-tolerant burkholderia vietnamiensis m6 isolated from ginseng rhizospheric soil. | we isolated and characterized novel insoluble phosphate (p)-solubilizing bacteria tolerant to environmental factors like high salt, low and high phs, and low temperature. a bacterium m6 was isolated from a ginseng rhizospheric soil and confirmed to belong to burkholderia vietnamiensis by biolog system and 16s rrna gene analysis. the optimal cultural conditions for the solubilization of p were 2.5% (w/v) glucose, 0.015% (w/v) urea, and 0.4% (w/v) mgcl(2).6h(2)o along with initial ph 7.0 at 35 deg ... | 2010 | 20024543 |
| improved electrotransformation and decreased antibiotic resistance of the cystic fibrosis pathogen burkholderia cenocepacia strain j2315. | the bacterium burkholderia cenocepacia is pathogenic for sufferers from cystic fibrosis (cf) and certain immunocompromised conditions. the b. cenocepacia strain most frequently isolated from cf patients, and which serves as the reference for cf epidemiology, is j2315. the j2315 genome is split into three chromosomes and one plasmid. the strain was sequenced several years ago, and its annotation has been released recently. this information should allow genetic experimentation with j2315, but two ... | 2010 | 20023084 |
| the rhizosphere and pah amendment mediate impacts on functional and structural bacterial diversity in sandy peat soil. | to reveal the degradation capacity of bacteria in pah polluted soil and rhizosphere we combined bacterial extradiol ring-cleavage dioxygenase and 16s rrna analysis in betula pubescens rhizoremediation. characterisation of the functional bacterial community by rflp revealed novel environmental dioxygenases, and their putative hosts were studied by 16s rrna amplification. plant rhizosphere and pah amendment effects were detected by the rflp/t-rflp analysis. functional species richness increased in ... | 2010 | 20022155 |
| genome-wide analysis of dna repeats in burkholderia cenocepacia j2315 identifies a novel adhesin-like gene unique to epidemic-associated strains of the et-12 lineage. | members of the burkholderia cepacia complex (bcc) are respiratory pathogens in patients with cystic fibrosis (cf). close repetitive dna sequences often associate with surface antigens to promote genetic variability in pathogenic bacteria. the genome of burkholderia cenocepacia j2315, a cf isolate belonging to the epidemic lineage edinburgh-toronto (et-12), was analysed for the presence of close repetitive dna sequences. among the 422 dna close repeats, 45 genes potentially involved in virulence ... | 2010 | 20019083 |
| burkholderia thailandensis harbors two identical rhl gene clusters responsible for the biosynthesis of rhamnolipids. | rhamnolipids are surface active molecules composed of rhamnose and beta-hydroxydecanoic acid. these biosurfactants are produced mainly by pseudomonas aeruginosa and have been thoroughly investigated since their early discovery. recently, they have attracted renewed attention because of their involvement in various multicellular behaviors. despite this high interest, only very few studies have focused on the production of rhamnolipids by burkholderia species. | 2009 | 20017946 |
| diversity and evolution of the phenazine biosynthesis pathway. | phenazines are versatile secondary metabolites of bacterial origin that function in biological control of plant pathogens and contribute to the ecological fitness and pathogenicity of the producing strains. in this study, we employed a collection of 94 strains having various geographic, environmental, and clinical origins to study the distribution and evolution of phenazine genes in members of the genera pseudomonas, burkholderia, pectobacterium, brevibacterium, and streptomyces. our results con ... | 2010 | 20008172 |
| burkholderia species are ancient symbionts of legumes. | burkholderia has only recently been recognized as a potential nitrogen-fixing symbiont of legumes, but we find that the origins of symbiosis in burkholderia are much deeper than previously suspected. we sampled 143 symbionts from 47 native species of mimosa across 1800 km in central brazil and found that 98% were burkholderia. gene sequences defined seven distinct and divergent species complexes within the genus burkholderia. the symbiosis-related genes formed deep burkholderia-specific clades, ... | 2010 | 20002602 |
| purification and characterization of vanillin dehydrogenases from alkaliphile micrococcus sp. ta1 and neutrophile burkholderia cepacia tm1. | vanillin dehydrogenases (vdhs) were purified and characterized from two bacterial strains that have different ph dependencies for growth. the alkaliphile micrococcus sp. ta1, isolated from an alkaline spa, can grow on several aromatic compounds such as ferulic acid, vanillin, vanillic acid, and protocatechuic acid under alkaline conditions. the neutrophile burkholderia cepacia tm1, which was isolated previously, also grew on the above-mentioned compounds because they functioned as the sole carbo ... | 2010 | 20002191 |
| efficacy of bacteriophage therapy in a model of burkholderia cenocepacia pulmonary infection. | the therapeutic potential of bacteriophages (phages) in a mouse model of acute burkholderia cenocepacia pulmonary infection was assessed. phage treatment was administered by either intranasal inhalation or intraperitoneal injection. bacterial density, macrophage inflammatory protein 2 (mip-2), and tumor necrosis factor alpha (tnf-alpha) levels were significantly reduced in lungs of mice treated with intraperitoneal phages (p < .05). no significant differences in lung bacterial density or mip-2 l ... | 2010 | 20001604 |
| user-loaded slipchip for equipment-free multiplexed nanoliter-scale experiments. | this paper describes a microfluidic approach to perform multiplexed nanoliter-scale experiments by combining a sample with multiple different reagents, each at multiple mixing ratios. this approach employs a user-loaded, equipment-free slipchip. the mixing ratios, characterized by diluting a fluorescent dye, could be controlled by the volume of each of the combined wells. the slipchip design was validated on an approximately 12 nl scale by screening the conditions for crystallization of glutaryl ... | 2010 | 20000708 |
| significance of infection markers and microbiological findings during tissue processing of cryopreserved arterial homografts for the early postoperative course. | to evaluate homograft implantation for the urgent treatment of vascular infections on the basis of the course of infection using microbiological findings in perioperatively obtained specimens and during homograft processing. | 2009 | 19998257 |
| activity of the siderophore monobactam bal30072 against multiresistant non-fermenters. | we tested the activity of bal30072, a novel siderophore monobactam, against multiresistant clinical isolates of pseudomonas aeruginosa, burkholderia cepacia group and acinetobacter spp. and against laboratory p. aeruginosa strains with defined resistance mechanisms. | 2010 | 19996139 |
| induction of immune response to the 17 kda ompa burkholderia cenocepacia polypeptide and protection against pulmonary infection in mice after nasal vaccination with an omp nanoemulsion-based vaccine. | burkholderia cepacia complex (bcc) are opportunistic bacteria associated with life-threatening illness in persons with cystic fibrosis. once bcc colonization is established, these antimicrobial-resistant and biofilm-forming bacteria are difficult to eradicate and are associated with increased rates of morbidity and mortality. at present, no vaccines are available to prevent the bcc infection. there is currently a paucity of published information regarding the development of vaccines designed to ... | 2010 | 19967396 |
| carboxymethyl-beta-cyclodextrin mitigates toxicity of cadmium, cobalt, and copper during naphthalene biodegradation. | hazardous waste sites are commonly contaminated with both organic and metal pollutants. many metal pollutants have been shown to inhibit organic pollutant biodegradation. we investigated the ability of a modified, polydentate cyclodextrin (carboxymethyl-beta-cyclodextrin, cmcd) to reduce the toxicity of 33.4 microm cadmium, cobalt or copper during naphthalene degradation by a burkholderia sp. in 120 h aerobic, batch studies. the highest investigated concentration of cmcd, 3340 microm, reduced ca ... | 2010 | 19962887 |
| natural history of glucose intolerance in patients with cystic fibrosis: ten-year prospective observation program. | to determine prospectively the long-term natural history of glucose homeostasis in adult patients with cystic fibrosis (cf). | 2010 | 19962154 |
| molecular epidemiology of glanders, pakistan. | we collected epidemiologic and molecular data from burkholderia mallei isolates from equines in punjab, pakistan from 1999 through 2007. we show that recent outbreaks are genetically distinct from available whole genome sequences and that these genotypes are persistent and ubiquitous in punjab, probably due to human-mediated movement of equines. | 2009 | 19961695 |
| susceptibility of caenorhabditis elegans to burkholderia infection depends on prior diet and secreted bacterial attractants. | the nematode caenorhabditis elegans may be killed by certain pathogenic bacteria and thus is a model organism for studying interactions between bacteria and animal hosts. however, growing nematodes on prey bacteria may influence their susceptibility to potential pathogens. a method of axenic nematode culture was developed to isolate and quantify interactions between c. elegans and potentially pathogenic strains of the burkholderia cepacia complex. studying these dynamics in liquid solution rathe ... | 2009 | 19956737 |
| production and characterization of poly-3-hydroxybutyrate from biodiesel-glycerol by burkholderia cepacia atcc 17759. | glycerol, a byproduct of the biodiesel industry, can be used by bacteria as an inexpensive carbon source for the production of value-added biodegradable polyhydroxyalkanoates (phas). burkholderia cepacia atcc 17759 synthesized poly-3-hydroxybutyrate (phb) from glycerol concentrations ranging from 3% to 9% (v/v). increasing the glycerol concentration results in a gradual reduction of biomass, pha yield, and molecular mass (m(n) and m(w)) of phb. the molecular mass of phb produced utilizing xylose ... | 2010 | 19953601 |
| distribution of cepacian biosynthesis genes among environmental and clinical burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions. | the genus burkholderia includes strains pathogenic to animals and plants, bioremediators, or plant growth promoters. genome sequence analyses of representative burkholderia cepacia complex (bcc) and non-bcc strains for the presence of the bce-i gene cluster, directing the biosynthesis of the exopolysaccharide (eps) cepacian, further extended this previously described cluster by another 9 genes. the genes in the bce-ii cluster were named bcem to bceu and encode products putatively involved in nuc ... | 2010 | 19948863 |
| rapid identification of nitrogen-fixing and legume-nodulating burkholderia species based on pcr 16s rrna species-specific oligonucleotides. | several novel n(2)-fixing burkholderia species associated with plants, including legume-nodulating species, have recently been discovered. presently, considerable interest exists in studying the diazotrophic burkholderia species, both for their ecology and their great potential for agro-biotechnological applications. however, the available methods used in the identification of these burkholderia species are time-consuming and expensive. in this study, pcr species-specific primers based on the 16 ... | 2010 | 19945811 |
| identification of immunogenic proteins from burkholderia cepacia secretome using proteomic analysis. | burkholderia cepacia is an opportunistic human pathogen associated with lung infections. secretory proteins of b. cepacia are known to be involved in virulence and may mediate important host-pathogen interactions. in the present study, secretory proteins isolated from b. cepacia culture supernatant were separated using two-dimensional gel electrophoresis, followed by western blot analysis to identify the immunogenic proteins. mice antibodies raised to b. cepacia inactivated whole bacteria, outer ... | 2010 | 19944788 |
| the hfq gene is required for stress resistance and full virulence of burkholderia cepacia to the nematode caenorhabditis elegans. | the burkholderia cepacia complex (bcc) emerged as problematic opportunistic pathogens to cystic fibrosis (cf) patients. although several virulence factors have been identified in bcc, the knowledge of their relative contribution to bcc pathogenicity remains scarce. in this work, we describe the identification and characterization of a b. cepacia ist408 mutant containing a disruption in the hfq gene. in other bacteria, hfq is a global regulator of metabolism, acting as an rna chaperone involved i ... | 2010 | 19942656 |
| toxin production by bacterial endosymbionts of a rhizopus microsporus strain used for tempe/sufu processing. | mould fungi are not only well known for food spoilage through toxin formation but also for the production of fermented foods. in asian countries, the fermentation of soy beans and tofu for tempe and sufu production with various rhizopus strains is widespread. here we report the finding of toxinogenic bacteria in a starter culture used for sufu production. by means of metabolic profiling of the fungus under standard conditions for tempe and sufu production, we found that toxins of the rhizoxin co ... | 2010 | 19942312 |
| novel tn4371-ice like element in ralstonia pickettii and genome mining for comparative elements. | integrative conjugative elements (ices) are important factors in the plasticity of microbial genomes. an element related to the ice tn4371 was discovered during a bioinformatic search of the ralstonia pickettii 12j genome. this element was analysed and further searches carried out for additional elements.a pcr method was designed to detect and characterise new elements of this type based on this scaffold and a culture collection of fifty-eight ralstonia pickettii and ralstonia insidiosa strains ... | 2009 | 19941653 |
| inactivation of burkholderia cepacia complex phage ks9 gp41 identifies the phage repressor and generates lytic virions. | the burkholderia cepacia complex (bcc) is made up of at least 17 species of gram-negative opportunistic bacterial pathogens that cause fatal infections in patients with cystic fibrosis and chronic granulomatous disease. ks9 (vb_bcens_ks9), one of a number of temperate phages isolated from bcc species, is a prophage of burkholderia pyrrocinia lmg 21824. transmission electron micrographs indicate that ks9 belongs to the family siphoviridae and exhibits the b1 morphotype. the 39,896-bp ks9 genome, ... | 2010 | 19939932 |
| inflammation in achromobacter xylosoxidans infected cystic fibrosis patients. | achromobacter xylosoxidans infection may cause conspicuous chronic pulmonary inflammation in cystic fibrosis (cf) patients similar to pseudomonas aeruginosa and the burkholderia cepacia complex (bcc). evolution in lung function was compared in chronically infected patients. cytokine concentrations in cf patients with and without chronic infection were compared to healthy controls. | 2010 | 19939747 |
| characterization of an endophytic bacterial community associated with eucalyptus spp. | endophytic bacteria were isolated from stems of eucalyptus spp (eucalyptus citriodora, e. grandis, e. urophylla, e. camaldulensis, e. torelliana, e. pellita, and a hybrid of e. grandis and e. urophylla) cultivated at two sites; they were characterized by rapd and amplified rdna restriction analysis (ardra). endophytic bacteria were more frequently isolated from e. grandis and e. pellita. the 76 isolates were identified by 16s rdna sequencing as erwinia/pantoea (45%), agrobacterium sp (21%), curt ... | 2009 | 19937585 |
| isolation and characterization of an abamectin-degrading burkholderia cepacia-like gb-01 strain. | abamectin is widely used in agriculture as an insecticide and in veterinary as an anti-parasitic agent, and has caused great environmental pollution by posing potential risk to non-target soil invertebrates and nearby aquatic systems. a bacterium designated gb-01, which was capable of degrading abamectin, was isolated from soil by enrichment culture method. on the basis of morphological, physiological and biochemical characteristics, combined with phylogenetic analysis of 16s rrna gene, the bact ... | 2010 | 19937266 |
| packed-bed reactor running on babassu oil and glycerol to produce monoglycerides by enzymatic route using immobilized burkholderia cepacia lipase. | the aim of this study was the glycerolysis of babassu oil catalyzed by immobilized lipase from burkholderia cepacia, in a continuous packed-bed reactor. the best reaction conditions were previously established in batchwise via response surface methodology as a function of glycerol-to-oil molar ratio and reaction temperature. the reactor operated continuously for 22 days at 50 degrees c, and during the first 6 days, no significant decrease on the initial lipase activity was observed. monoglycerid ... | 2010 | 19937155 |
| structural elucidation of the repeat unit in highly branched acidic exopolysaccharides produced by nitrogen fixing burkholderia. | burkholderia kururiensis, strain m130, an endophytic diazotrophic bacterium isolated from rice roots, produces acetylated acidic exopolysaccharides which can be separated by anion exchange chromatography. these were characterized by nuclear magnetic resonance spectroscopy, methylation analysis and smith degradation. the exopolysaccharides eluted with 0.5 m nacl were produced when the bacterium was grown in a medium containing mannitol as the sole carbon source, and showed to be a mixture of two ... | 2010 | 19933228 |
| can burkholderia cepacia complex be eradicated with nebulised amiloride and tobi? | 2010 | 19932062 | |
| expression and characterization of penicillin-binding proteins in burkholderia cenocepacia. | putative penicillin-binding proteins (pbps) were identified in the genome of the burkholderia cenocepacia strain j2315 based on homology to e. coli pbps. the three sequences identified as homologs of e. coli pbp1a, bcal2021, bcal0274, and bcam2632, were cloned and expressed as his(6)-tagged fusion proteins in e. coli. the fusion proteins were isolated and shown to bind beta-lactams, indicating these putative pbps have penicillin-binding activity. | 2010 | 19924480 |
| a preliminary x-ray study of sedoheptulose-7-phosphate isomerase from burkholderia pseudomallei. | sedoheptulose-7-phosphate isomerase (gmha) converts d-sedoheptulose 7-phosphate to d,d-heptose 7-phosphate. this is the first step in the biosynthesis pathway of ndp-heptose, which is responsible for the pleiotropic phenotype. this biosynthesis pathway is the target of inhibitors to increase the membrane permeability of gram-negative pathogens or of adjuvants working synergistically with known antibiotics. burkholderia pseudomallei is the causative agent of melioidosis, a seriously invasive dise ... | 2009 | 19923728 |
| molecular evolution in bacterial endosymbionts of fungi. | the prediction that progressive coupling of host and symbiont metabolic and reproductive interests leads to reduced mixing of symbiont lineages has been verified extensively in maternally transmitted bacterial endosymbionts of insects. to test whether this prediction is also applicable to associations of bacteria with fungi, we explored patterns of molecular evolution in two lineages of mutualistic endosymbionts of fungi: the burkholderia endosymbionts of rhizopus microsporus (mucormycotina) and ... | 2010 | 19923192 |
| phylogeographic reconstruction of a bacterial species with high levels of lateral gene transfer. | phylogeographic reconstruction of some bacterial populations is hindered by low diversity coupled with high levels of lateral gene transfer. a comparison of recombination levels and diversity at seven housekeeping genes for eleven bacterial species, most of which are commonly cited as having high levels of lateral gene transfer shows that the relative contributions of homologous recombination versus mutation for burkholderia pseudomallei is over two times higher than for streptococcus pneumoniae ... | 2009 | 19922616 |
| diabetes as a determinant of mortality in cystic fibrosis. | diabetes is increasingly common in cystic fibrosis, but little information describing its influence on mortality exists. using national u.k. data, in this study we document diabetes-specific mortality rates, estimate the impact of diabetes on survival, and estimate population-attributable fractions. | 2010 | 19918014 |
| characterization of chlorophenol 4-monooxygenase (tftd) and nadh:fad oxidoreductase (tftc) of burkholderia cepacia ac1100. | burkholderia cepacia ac1100 completely degrades 2,4,5-trichlorophenol, in which an fadh(2)-dependent monooxygenase (tftd) and an nadh:fad oxidoreductase (tftc) catalyze the initial steps. tftd oxidizes 2,4,5-trichlorophenol (2,4,5-tcp) to 2,5-dichloro-p-benzoquinone, which is chemically reduced to 2,5-dichloro-p-hydroquinone (2,5-dichq). then, tftd oxidizes the latter to 5-chloro-2-hydroxy-p-benzoquinone. in those processes, tftc provides all the required fadh(2). we have determined the crystal ... | 2010 | 19915006 |
| entry of burkholderia organisms into respiratory epithelium: cftr, microfilament and microtubule dependence. | the pathogenesis of infection with burkholderia cepacia complex (bcc) organisms may be linked to its capacity to invade respiratory epithelium. | 2010 | 19914879 |
| colloidal and bacterial deposition: role of gravity. | the role of gravitational force on the deposition of 0.5, 1.1, and 1.8 mum carboxylate-modified polystyrene latex (cml) microspheres and bacterium burkholderia cepacia g4g has been evaluated using a parallel plate flow chamber system. this experimental system utilized an inverted and an upright optical microscope attached with image-capturing devices to directly observe and determine the deposition kinetics onto glass surfaces located at the top and bottom of the flow chamber. deposition kinetic ... | 2010 | 19911823 |
| genomic transcriptional profiling identifies a candidate blood biomarker signature for the diagnosis of septicemic melioidosis. | melioidosis is a severe infectious disease caused by burkholderia pseudomallei, a gram-negative bacillus classified by the national institute of allergy and infectious diseases (niaid) as a category b priority agent. septicemia is the most common presentation of the disease with a 40% mortality rate even with appropriate treatments. better diagnostic tests are therefore needed to improve therapeutic efficacy and survival rates. | 2009 | 19903332 |
| false-positive widal in melioidosis. | enteric fever is endemic in this part of the world, and widal test is one of the time-honored laboratory tests that are being used for years to diagnose the disease. on the other hand, melioidosis is a newly emerging disease from this region, which is most often misdiagnosed or underdiagnosed by clinicians. it is well accepted that false-positive widal reactions following certain non-typhoid salmonella infections may occur commonly. three cases of high titers of widal test are described, where m ... | 2009 | 19901486 |
| cloning and overexpression of the xylose isomerase gene from burkholderia sacchari and production of polyhydroxybutyrate from xylose. | a different organization for the xyl operon was found in different genomes of burkholderia and pseudomomas species. degenerated primers were designed based on burkholderia genomes and used to amplify the xylose isomerase gene (xyla) from burkholderia sacchari ipt101. the gene encoded a protein of 329 amino acids, which showed the highest similarity (90%) to the homologous gene of burkholderia dolosa. it was cloned in the broad host range plasmid pbbr1mcs-2, which partially restored growth and po ... | 2009 | 19898542 |
| mycotic iliac aneurysm associated with burkholderia pseudomallei. | 2010 | 19897393 | |
| [a sensitive, specific and predictive isolation medium developed for stenotrophomonas maltophilia study in healthcare settings]. | stenotrophomonas maltophilia (smalto) is a prominent nosocomial pathogen, commonly isolated in the hospital environment. multiple smalto nosocomial outbreaks have been linked to contaminated water sources. this study aimed to develop a medium able to ease healthcare environment smalto isolation. | 2010 | 19892479 |
| imported melioidosis, israel, 2008. | in 2008, melioidosis was diagnosed in an agricultural worker from thailand in the southern jordan valley in israel. he had newly diagnosed diabetes mellitus, fever, multiple abscesses, and osteomyelitis. burkholderia pseudomallei was isolated from urine and blood. four of 10 laboratory staff members exposed to the organism received chemoprophylaxis, 3 of whom had adverse events. | 2009 | 19891871 |
| burkholderia pseudomallei misidentified by automated system. | after returning from thailand, a 35-year-old man from switzerland was hospitalized with an abscess of the head. material cultured from the abscess and adjacent bone grew a gram-negative rod, which was misidentified by an automated microbiology system as burkholderia cepacia. the organism was eventually identified by molecular methods as b. pseudomallei. | 2009 | 19891868 |
| oxidative stress of burkholderia cenocepacia induces insertion sequence-mediated genomic rearrangements that interfere with macrorestriction-based genotyping. | burkholderia cenocepacia can cause serious infections and epidemics in patients with cystic fibrosis (cf). a cf population in the czech republic experienced an epidemic outbreak caused by a b. cenocepacia st-32 strain. the clonality of the isolates was evident by multilocus sequence typing; however, fingerprinting profiles obtained by pulsed-field gel electrophoresis (pfge) showed substantial band variability. we investigated whether the pfge pattern diversity resulted from genomic rearrangement ... | 2010 | 19889907 |
| backbone and side chain (1)h, (13)c, and (15)n nmr assignments for the organic hydroperoxide resistance protein (ohr) from burkholderia pseudomallei. | burkholderia pseudomallei is a niaid category b microorganism responsible for melioidosis. here we report backbone and side chain nmr assignments for the 139-residue, homodimeric, organic hydroperoxide resistance protein (ohr) from this organism. | 2009 | 19888681 |
| computational study of the lipase-mediated desymmetrisation of 2-substituted-propane-1,3-diamines. | the enantioselectivity displayed by the lipase from pseudomonas cepacia towards a wide range of prochiral 2-substituted-propane-1,3-diamines was studied by means of molecular dynamics simulations (mds). in all cases the enzyme allows the recovery of the corresponding amino carbamates of r configuration. however, the enantioselectivity is only synthetically useful if no ortho substituent is present and the aromatic ring is directly bonded to the 2-carbon of the 1,3-diamine core. analysis of the m ... | 2009 | 19885896 |
| effects of nutrients on quorum signals and secondary metabolite productions of burkholderia sp. o33. | several bioactive metabolites, including pyrrolnitrin, nacylhomoserine lactones, and polyhydroxyalkanoates were isolated from burkholderia sp. o33. effects of various nutrients, including sugars, gluconolactone, glycerol, tryptophan, chloride, and zinc were investigated in relation to the production of these metabolites. logarithmic increase of pyrrolnitrin was observed between 2-5 days and reached a maximum at 7-10 days. tryptophan concentration reached the maximum at 3 days, whereas 7-chlorotr ... | 2009 | 19884772 |
| molecular and biochemical characterization of the natural chromosome-encoded class a beta-lactamase from pseudomonas luteola. | pseudomonas luteola (formerly classified as cdc group ve-1 and named chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. a novel beta-lactamase gene, bla(lut-1), was cloned from the whole-cell dna of the p. luteola clinical isolate lam, which had a weak narrow-spectrum beta-lactam-resistant phenotype, and expressed in escherichia coli. this gene encoded lut-1, a 296-amino-acid ambler class a beta-lactamase with a pi of 6 and a theoretical molecular m ... | 2010 | 19884377 |
| burkholderia mallei cluster 1 type vi secretion mutants exhibit growth and actin polymerization defects in raw 264.7 murine macrophages. | burkholderia mallei is a facultative intracellular pathogen that causes severe disease in animals and humans. recent studies have shown that the cluster 1 type vi secretion system (t6ss-1) expressed by this organism is essential for survival in a hamster model of glanders. to better understand the role of t6ss-1 in the pathogenesis of disease, studies were initiated to examine the interactions of b. mallei tsse mutants with raw 264.7 murine macrophages. results obtained by utilizing modified gen ... | 2010 | 19884331 |
| the lipid a of burkholderia multivorans c1576 smooth-type lipopolysaccharide and its pro-inflammatory activity in a cystic fibrosis airways model. | cystic fibrosis is an autosomal recessive disorder and it is characterised by chronic bacterial airway infection which leads to progressive lung deterioration, sometimes with fatal outcome. burkholderia multivorans and burkholderia cenocepacia are the species responsible for most of the infections of cystic fibrosis patients. lipopolysaccharide endotoxins (lpss) are among the foremost factors of pathogenesis of gram-negative infection and, in particular, lipid a is the endotoxic portion of lps r ... | 2010 | 19880661 |
| topological analysis of a haloacid permease of a burkholderia sp. bacterium with a phoa-lacz reporter. | 2-haloacids can be found in the natural environment as degradative products of natural and synthetic halogenated compounds. they can also be generated by disinfection of water and have been shown to be mutagenic and to inhibit glyceraldehyde-3-phosphate dehydrogenase activity. we have recently identified a novel haloacid permease deh4p from a bromoacetate-degrading bacterium burkholderia sp. mba4. comparative analyses suggested that deh4p is a member of the major facilitator superfamily (mfs), w ... | 2009 | 19878597 |
| the active site of an enzyme can host both enantiomers of a racemic ligand simultaneously. | 2009 | 19876985 | |
| [doripenem: need for a new carbapenem]. | doripenem is a new member of the carbapenem family. its spectrum is a little wider than meropenem and it is active on some pseudomonas aeruginosa strains resistant to other carbapenems and on burkholderia cepacia. doripenem was evaluated in nosocomial pneumonia including ventilator-acquired pneumonia, complicated intra-abdominal infection, and complicated urinary tract infection. in nosocomial pneumonia, doripenem showed an interesting activity in p. aeruginosa infected patients. this data, alon ... | 2009 | 19875257 |
| icmf is a fusion between the radical b12 enzyme isobutyryl-coa mutase and its g-protein chaperone. | coenzyme b(12) is used by two highly similar radical enzymes, which catalyze carbon skeleton rearrangements, methylmalonyl-coa mutase and isobutyryl-coa mutase (icm). icm catalyzes the reversible interconversion of isobutyryl-coa and n-butyryl-coa and exists as a heterotetramer. in this study, we have identified >70 bacterial proteins, which represent fusions between the subunits of icm and a p-loop gtpase and are currently misannotated as methylmalonyl-coa mutases. we designate this fusion prot ... | 2010 | 19864421 |
| differential susceptibility of inbred mouse strains to burkholderia thailandensis aerosol infection. | burkholderia pseudomallei is the causative agent of melioidosis, an emerging bacterial disease that accounts for high rates of septicaemia and death in parts of southeast asia and northern australia. the closely related species burkholderia thailandensis is considered avirulent in humans and has been used as a surrogate for b. pseudomallei in several studies. the pathogenesis of b. pseudomallei and the role of toll-like receptors (tlrs) in host immunity to infection are not well-defined. in this ... | 2010 | 19853031 |
| pahang melioidosis registry. | melioidosis has a high annual incidence and mortality rate in pahang, malaysia. we initiated the first melioidosis registry in the country on 1st july 2005 to improve the management of melioidosis in the state. continuous medical education on melioidosis was carried out in all hospitals in the state to highlight the magnitude of the disease and to educate the doctors on the treatment of the disease. all culture confirmed cases were registered and analysed. during the one-year study period from 1 ... | 2009 | 19852316 |
| biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic burkholderia sp. is-01. | burkholderia sp. is-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3hb-co-3hv)] copolyesters with a high molar fraction of 3hv was isolated from the gut of the adult longicorn beetle, moechotypa diphysis. the strain is-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3hb-co-86.5 mol% 3hv) copolyester when cultivated on a mixture of gluconate (20 g/l) and levulinic acid (12.5 g/l). in this case, the content of t ... | 2009 | 19851740 |
| purification and crystallization of a putative transcriptional regulator of the benzoate oxidation pathway in burkholderia xenovorans lb400. | burkholderia xenovorans lb400 harbours two paralogous copies of the recently discovered benzoate oxidation (box) pathway. while both copies are functional, the paralogues are differentially regulated and flanked by putative transcriptional regulators from distinct families. the putative lysr-type transcriptional regulator (lttr) adjacent to the megaplasmid-encoded box enzymes, bxe_c0898, has been produced recombinantly in escherichia coli and purified to homogeneity. gel-filtration studies show ... | 2009 | 19851006 |
| the clinical profile and predictors of mortality in patients with melioidosis in south india. | melioidosis is an underdiagnosed and underreported disease in india with protean clinical manifestations. mortality in this study population was 17%. the predominant risk factor for melioidosis was diabetes mellitus. multifocal disease was present in 66% and pulmonary involvement in 61% of patients. in a country like india where the conditions are conducive for endemic melioidosis and due to the clinical similarity of melioidosis to diseases like tuberculosis, it is essential for clinicians to h ... | 2010 | 19850605 |
| pathema: a clade-specific bioinformatics resource center for pathogen research. | pathema (http://pathema.jcvi.org) is one of the eight bioinformatics resource centers (brcs) funded by the national institute of allergy and infectious disease (niaid) designed to serve as a core resource for the bio-defense and infectious disease research community. pathema strives to support basic research and accelerate scientific progress for understanding, detecting, diagnosing and treating an established set of six target niaid category a-c pathogens: category a priority pathogens; bacillu ... | 2010 | 19843611 |
| lack of correlation of burkholderia pseudomallei quantities in blood, urine, sputum and pus. | we evaluated the correlation of burkholderia pseudomallei quantities in blood versus urine, sputum or pus. correlations between bacterial counts in blood and other samples were not found. it is likely that an initial seeding event to extracellular organs is followed by independent growth of b. pseudomallei, and that bacteria in the urine were not passively filtered from the bloodstream. | 2009 | 19842414 |
| mixotrophic metabolism in burkholderia kururiensis subsp. thiooxydans subsp. nov., a facultative chemolithoautotrophic thiosulfate oxidizing bacterium isolated from rhizosphere soil and proposal for classification of the type strain of burkholderia kururiensis as burkholderia kururiensis subsp. kururiensis subsp. nov. | a thiosulfate-oxidizing facultative chemolithoautotrophic burkholderia sp. strain atsb13(t) was previously isolated from rhizosphere soil of tobacco plant. strain atsb13(t) was aerobic, gram-staining-negative, rod shaped and motile by means of sub-terminal flagellum. strain atsb13(t) exhibited mixotrophic growth in a medium containing thiosulfate plus acetate. a phylogenetic study based on 16s rrna gene sequence analysis indicated that strain atsb13(t) was most closely related to burkholderia ku ... | 2009 | 19841903 |
| identifying translation initiation sites in prokaryotes using support vector machine. | motivation: gene identification in genomes has been a fundamental and long-standing task in bioinformatics and computational biology. many computational methods have been developed to predict genes in prokaryote genomes by identifying translation initiation site (tis) in transcript data. however, the pseudo-tiss at the genome level make these methods suffer from a high number of false positive predictions. in addition, most of the existing tools use an unsupervised learning framework, whose pred ... | 2010 | 19840808 |
| pip(2)-binding site in kir channels: definition by multiscale biomolecular simulations. | phosphatidylinositol bisphosphate (pip(2)) is an activator of mammalian inwardly rectifying potassium (kir) channels. multiscale simulations, via a sequential combination of coarse-grained and atomistic molecular dynamics, enabled exploration of the interactions of pip(2) molecules within the inner leaflet of a lipid bilayer membrane with possible binding sites on kir channels. three kir channel structures were investigated: x-ray structures of kirbac1.1 and of a kir3.1-kirbac1.3 chimera and a h ... | 2009 | 19839652 |
| terminal-restriction fragment length polymorphism analysis of biphenyl dioxygenase genes from a polychlorinated biphenyl-polluted soil. | polychlorinated biphenyls (pcbs) are ubiquitous persistent organic pollutants that can be co-metabolically biotransformed by biphenyl-utilizing bacteria. in this study, terminal-restriction fragment length polymorphism (t-rflp) was applied to the substrate specificity-determining region of the 2,3-biphenyl dioxygenase encoding genes of a microbial community found in a pcb-polluted soil. notably, both the total biphenyl/pcb-utilizing community and its members actively expressing the 2,3-biphenyl ... | 2009 | 19835952 |
| regulation of phenylacetic acid degradation genes of burkholderia cenocepacia k56-2. | metabolically versatile soil bacteria burkholderia cepacia complex (bcc) have emerged as opportunistic pathogens, especially of cystic fibrosis (cf). previously, we initiated the characterization of the phenylacetic acid (pa) degradation pathway in b. cenocepacia, a member of the bcc, and demonstrated the necessity of a functional pa catabolic pathway for full virulence in caenorhabditis elegans. in this study, we aimed to characterize regulatory elements and nutritional requirements that contro ... | 2009 | 19835630 |
| [phenanthren biodegradation and interaction of pseudomonas putida bs3701 and burkholderia sp.bs3702 in plant rhizosphere]. | 2009 | 19827713 | |
| use of a low-dose steroid as an adjunct in the treatment, in mice, of severe sepsis caused by burkholderia pseudomallei. | human melioidosis caused by burkholderia pseudomallei is a severe septic disease that is associated with high mortality, even under appropriate antibiotic treatment. the therapeutic effects of low-dose hydrocortisone plus ceftazidime, and of ceftazidime alone, have recently been investigated in the treatment of acute, severe sepsis caused by b. pseudomallei, both in normal balb/c mice and in balb/c mice with streptozotocin-induced diabetes. the mice were infected and then treated intravenously, ... | 2009 | 19825285 |
| burkholderia acidipaludis sp. nov., aluminum-tolerant bacteria isolated from chinese water chestnut (eleocharis dulcis) growing in highly acidic swamps in south-east asia. | two strains of aluminium-tolerant bacteria, sa33(t) and 7a078, were isolated from chinese water chestnut (eleocharis dulcis) growing in highly acidic swamps (ph 2-4) in actual acid sulfate soil areas of vietnam (sa33(t)) and thailand (7a078). the strains were gram-negative, aerobic, non-spore-forming rods, 0.6-0.7 mum wide and 1.3-1.7 mum long. these strains showed good growth at ph 3.0-8.0 and 17-37 degrees c. the organisms contained ubiquinone q-8 as the predominant isoprenoid quinone and c(16 ... | 2010 | 19819996 |