Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| effect of relative humidity and interrupted wetness periods on brown spot severity of pear caused by stemphylium vesicarium. | abstract field observations in four pear orchards during 5 years from april to october indicated that days with uninterrupted wetness of variable length represented 83.9% of the total days studied. however, days with surface wetness interruptions and with high relative humidity (rh) (>/=90%) without wetness occurred with a frequency of 7.1 and 6.2%, respectively. accordingly, the effect of interruption of 24-h wetness periods by dry periods of high or low rh on infections caused by stemphylium v ... | 2002 | 18944145 |
| genetic analysis of a pathogenic erwinia sp. isolated from pear in japan. | abstract four erwinia strains, originally isolated in japan from pear trees with bacterial shoot blight symptoms, were analyzed to determine their genetic relationship with erwinia amylovora and e. pyrifoliae. when genomes were characterized with amplified fragment length polymorphism markers and by comparative groel sequence analysis, the japanese erwinia sp. and south korean e. pyrifoliae strains were placed in the same group, which was phylogenetically distinct from a group of 15 strains of e ... | 2003 | 18944067 |
| improved control of postharvest decay of pears by the combination of candida sake (cpa-1) and ammonium molybdate. | abstract the potential enhancement of candida sake (cpa-1) by ammonium molybdate to control blue and gray mold caused by penicillium expansum and botrytis cinerea, respectively, on blanquilla pears was investigated. in laboratory trials, improved control of blue and gray molds was obtained with the application of ammonium molybdate (1, 5, 10, and 15 mm) alone or in combination with c. sake at 2 x 10(6) or 2 x 10(7) cfu ml(-1) on blanquilla pears stored at 20 degrees c. in semicommercial trials a ... | 2002 | 18944000 |
| testing a rapid diagnostic medium for erwinia amylovora and development of a procedure for sampling blossoms in pear orchards. | abstract the coliform agar produced by merck was tested for rapid diagnosis of erwinia amylovora (the causal agent of fire blight) in pear blossoms. the medium enabled the diagnosis to be completed within 36 h. diagnoses performed with the medium were confirmed by the biolog and the fatty-acid profile methods. the diagnostic medium was used to determine the spatial distribution of colonized blossoms in the orchards and it was found that e. amylovora may be distributed both in clusters and at ran ... | 2003 | 18943859 |
| an indigenous virulent strain of erwinia amylovora lacking the ubiquitous plasmid pea29. | abstract an atypical strain of erwinia amylovora was isolated near an outbreak of fire blight at a nursery in spain in 1996. it was obtained from a crataegus plant showing typical symptoms and was identified as e. amy-lovora by biochemical tests and enrichment-enzyme-linked immuno-sorbent assay, but not by polymerase chain reaction using primers based on the pea29 sequence. nevertheless, with primers from chromosomal regions, the isolate gave the expected amplification band. this strain carries ... | 2006 | 18943756 |
| dual activity of a viral lysozyme with high efficiency for growth inhibition of erwinia amylovora. | abstract the lysozyme from erwinia amylovora phage phiea1h was investigated for its ability to inhibit growth of bacteria and compared with the lysozyme from escherichia coli phage t4. the assays to measure lysozyme activity included cell lysis and growth inhibition of bacteria. bacterial strains with kanamycin resistance were not affected by lysates containing the phiea1h-enzyme. the titer of micrococcus luteus but not of erwinia amylovora was diminished by cell extracts containing t4 lysozyme. ... | 2004 | 18943701 |
| bioavailability of iron to pseudomonas fluorescens strain a506 on flowers of pear and apple. | abstract the addition of 0.1 mm fecl(3) to a defined culture medium induces the bacterial epiphyte pseudomonas fluorescens strain a506 (a506) to produce an antibiotic toxic to the fire blight pathogen, erwinia amylovora. consequently, because a506 is registered and applied as a commercial product to suppress e. amylovora before floral infection of pear and apple, the relative availability of iron to a506 on surfaces of pear and apple flowers is of potential significance. an 'iron biosensor' cons ... | 2004 | 18943698 |
| detection, distribution, sporulation, and survival of phytophthora ramorum in a california redwood-tanoak forest soil. | abstract recovery of phytophthora ramorum from soils throughout sudden oak death-affected regions of california illustrates that soil may serve as an inoculum reservoir, but the role of soil inoculum in the disease cycle is unknown. this study addresses the efficacy of soil baiting, seasonal pathogen distribution under several epidemiologically important host species, summer survival and chlamydospore production in soil, and the impact of soil drying on pathogen survival. the efficacy of rhodode ... | 2007 | 18943696 |
| evaluation of likelihood of co-occurrence of erwinia amylovora with mature fruit of winter pear. | abstract phytosanitary concerns about fire blight prohibit export of u.s.-grown pears to some countries without this disease. to examine these concerns, we evaluated the potential for co-occurrence of erwinia amylovora with mature, symptomless winter pear fruit by inoculation experiments and by survey of commercial orchards. immature pear and apple fruit were inoculated in orchards with e. amylovora strain 153n as resuspended lyophilized cells or as ooze from diseased tissues. regardless of inoc ... | 2007 | 18943684 |
| analysis of aggressiveness of erwinia amylovora using disease-dose and time relationships. | abstract the aggressiveness of an extensive collection of strains of erwinia amylovora was analyzed using immature fruit and detached pear flower assays under controlled environmental conditions. the analysis was performed by means of a quantitative approach based on fitting data to mathematical models that relate infection incidence to pathogen dose and time. probit and hyperbolic saturation models were used for disease-dose relationships and provided information on the median effective dose (e ... | 2005 | 18943554 |
| genetic diversity of phytophthora infestans sensu lato in ecuador provides new insight into the origin of this important plant pathogen. | abstract the metapopulation structure of phytophthora infestans sensu lato is genetically diverse in the highlands of ecuador. previous reports documented the diversity associated with four putative clonal lineages of the pathogen collected from various hosts in the genus solanum. this paper simultaneously analyzes diversity of the complete collection of isolates, including a large number that had not yet been reported. this analysis confirmed the existence of three pathogen populations, which a ... | 2004 | 18943538 |
| acibenzolar-s-methyl-induced resistance to japanese pear scab is associated with potentiation of multiple defense responses. | abstract this study reports the mode of action of acibenzolar-s-methyl (asm) against japanese pear scab, caused by venturia nashicola. pretreatment of potted japanese pear trees with asm reduced scab symptoms and potentiated several lines of plant defense response. this included transcripts encoding polygalacturonase-inhibiting protein (pgip) that were highly and transiently promoted after scab inoculation of plants pretreated with asm, suggesting a possible role for defenses involved in direct ... | 2004 | 18943485 |
| antibiosis and acidification by pantoea agglomerans strain e325 may contribute to suppression of erwinia amylovora. | pantoea agglomerans strain e325, a commercially available antagonist for fire blight of apple and pear, was originally selected through screening based on suppression of erwinia amylovora on flower stigmas, but specific mechanisms of antagonism were unknown. bacterial modification of ph was evaluated as a possible mechanism by analyzing stigma exudates extracted from 'gala' apple stigmas. the ph values for field samples were only slightly lower than controls, but indicated a range (ph 5 to 6) co ... | 2008 | 18943460 |
| the role of water in epiphytic colonization and infection of pomaceous flowers by erwinia amylovora. | abstract detached crab apple flowers were used as an experimental model to investigate the effect of relative humidity (rh), free moisture, and water potential psi(w) on the interaction between erwinia amylovora and pomaceous flowers. flowers were maintained at 24 degrees c with the cut pedicel submerged in a sucrose solution. the bacterium multiplied on inoculated flower stigmas at between approximately 55 and 100% rh but not in the floral cup (hypanthium) until the rh was higher than 80%. to s ... | 2000 | 18943376 |
| influence of nutrient and environmental factors on conidial germination of potebniamyces pyri. | abstract potebniamyces pyri is the causal agent of phacidiopycnis rot, a postharvest disease of pears. infection of fruit occurs in the orchard, and symptoms develop during storage. conidial germination of p. pyri in response to nutrient, temperature, wetness duration, relative humidity (rh), and ph was determined in vitro. conidia germinated by either budding or developing germ tubes in various concentrations of pear juice solutions. the mode of conidial germination was nutrient-dependent. low ... | 2005 | 18943324 |
| characterization of phytophthora capsici from michigan surface irrigation water. | abstract phytophthora capsici infects cucurbitaceous and solanaceous crops worldwide. in free water, p. capsici sporangia release zoospores that may be disseminated by moving surface water. surface irrigation sources (river system, ponds, and ditches) in three michigan counties with a history of p. capsici-susceptible crop production were monitored for the pathogen during four growing seasons (2002 to 2005). pear and cucumber baits were suspended in water at monitoring sites for 3- to 7-day inte ... | 2007 | 18943282 |
| worldwide phylogeography of the citrus brown spot pathogen, alternaria alternata. | abstract sixty-five isolates of alternaria alternata were sampled from brown spot lesions on tangerines and mandarins (citrus reticulata) and tangerine x grapefruit (c. reticulata x c. paradisi) hybrids in the united states, colombia, australia, turkey, south africa, and israel to investigate the worldwide phylogeography of the fungus. genetic variation was scored at 15 putative random amplified polymorphic dna (rapd) loci and 465 bp of an endo-polygalacturonase (endo-pg) gene was sequenced for ... | 2002 | 18943277 |
| ultrastructural study on acibenzolar-s-methyl-induced scab resistance in epidermal pectin layers of japanese pear leaves. | the infection behavior of japanese pear scab pathogen venturia nashicola race 1 was studied ultrastructurally in acibenzolar-s-methyl (asm)-pretreated susceptible japanese pear (cv. kousui) leaves to determine the mechanism of asm-induced scab resistance. on asm-pretreated leaf surfaces, the infection behavior (conidial germination and appressorial formation) was similar to that on distilled water (dw)-pretreated leaves prior to cuticle penetration by the pathogen. however, after penetration, di ... | 2008 | 18943227 |
| temperature and pomaceous flower age related to colonization by erwinia amylovora and antagonists. | abstract fire blight of apple and pear is initiated by epiphytic populations of erwinia amylovora on flower stigmas. predicting this disease and managing it with microbial antagonists depends on an understanding of bacterial colonization on stigmas. detached 'manchurian' crab apple flowers were inoculated with e. amylovora and subjected to a range of constant temperatures or various fluctuating temperature regimes. results may have application to disease risk assessment systems such as the couga ... | 2004 | 18943112 |
| temporal dynamics of the biocontrol agent pseudomonas fluorescens strain a506 in flowers in inoculated pear trees. | abstract the colonization of individual flowers in mature pear orchards by pseudomonas fluorescens strain a506 applied at different times during bloom was measured to determine the receptivity of flowers to colonization and the extent of intra-tree movement over time. strain a506 populations in flowers open at inoculation were initially about 10(4) cells per flower and increased to approximately 10(6) cells per flower in flowers that were inoculated within about 5 days of opening. however, event ... | 2003 | 18943060 |
| medicinal agents in the metabolic syndrome. | the metabolic syndrome (ms) has become a worldwide health problem. it is difficult for patients to follow a diet/exercise regime that would improve their symptoms, therefore the investigation of agents that may deal with its more serious aspects is an important medical field for research. the cardiovascular consequences associated with the syndrome and some of the therapeutic approaches are discussed. the different agents can be divided into several groups: inorganic/ organic: zinc complexes wit ... | 2008 | 18855636 |
| [gorham-stout disease]. | gorham-stout disease is a rare entity characterised by a massive osteolysis and typically starts monocentrically in an isolated bone. concerning the pathogenesis, haemangiomatosis and an overstimulation of osteoclasts are discussed which cause a progressive destruction of the affected skeleton and involve neighbouring soft tissue in the further progress of the disease. | 2008 | 18846495 |
| the c-terminal half of the hrpn virulence protein of the fire blight pathogen erwinia amylovora is essential for its secretion and for its virulence and avirulence activities. | the hrpn (harpin) protein of the fire blight pathogen erwinia amylovora is an essential virulence factor secreted via the bacterial type iii secretion system. hrpn also has avirulence activity when delivered to tobacco by e. amylovora and has defense elicitor activity when applied to plants as a cell-free protein extract. here, we characterize a series of random mutations in hrpn that altered the predicted amino acid sequence of the protein. amino acid substitutions and deletions in the highly c ... | 2008 | 18842089 |
| an antibacterial pyrazole derivative from burkholderia glumae, a bacterial pathogen of rice. | burkholderia glumae, a bacterial pathogen on rice, produced compounds in liquid culture that, in agar diffusion assays, gave strong inhibitory action against erwinia amylovora, the bacterium responsible for fire blight disease of apple and pear trees. products were isolated from culture medium by cation exchange and then purified by bioassay-guided chromatographic methods. two major products were obtained, one of which was not active when fully purified. each product showed a single ninhydrin-st ... | 2008 | 18834606 |
| the luxs gene is involved in ai-2 production, pathogenicity, and some phenotypes in erwinia amylovora. | erwinia amylovora causes fire blight of apple, pear, and other members of the rosaceae family. the enzyme luxs catalyzes the last step in the production of autoinducer-2 (ai-2), a molecule implicated with quorum sensing in many bacterial species. it is now well recognized that luxs also plays a central role in sulfur metabolism and in the activated methyl cycle, which is responsible for the generation of s-adenosyl-l-methionine. a research paper has reported that luxs is not involved with quorum ... | 2009 | 18820970 |
| benefit of a 17-year long-term bisphosphonate therapy in a patient with gorham-stout syndrome. | this case report of a 61-year-old woman suffering from gorham-stout syndrome shows osteolyses of the left pelvis, proximal femur and lumbar spine. the therapeutic regime has included two courses of percutaneous radiotherapy and also continuous application of bisphosphonates over 17 years. despite this antiresorptive therapy, elevated urinary excretion of desoxypyridinoline has indicated the persistence of increased bone destruction. the radiological progression following bisphosphonate treatment ... | 2009 | 18807053 |
| field evaluations of concentrated spray applications of microencapsulated sex pheromone for codling moth (lepidoptera: tortricidae). | the application of a microencapsulated (mec) sex pheromone formulation (checkmate cm-f) for codling moth, cydia pomonella (l.), in low volume, concentrated sprays was evaluated in a series of small plot and grower trials in apple, malus domestica borkhausen, and pear, pyrus communis l. preliminary tests found that mec sprays applied at 172-207 kilopascals in 12-23 liters/ha deposited the highest density of microcapsules per leaf. the addition of a latex sicker did not increase the deposition of ... | 2008 | 18801264 |
| [application of icp-ms to detection of trace elements and heavy metals in different kinds of honey]. | honey contains abundant trace elements, which are important nutritional elements for human health, but the difference between different species has not been reported. seven kinds of trace elements and five kinds of heavy metals in different honey species were analyzed by the method of icp-ms. the results showed that honey contained many wholesome elements, such as ca, fe, zn, mn, cu, se and mo, but there was great difference between different species. the highest concentrations of ca, fe, zn, mn ... | 2008 | 18800735 |
| red 'anjou' pear has a higher photoprotective capacity than green 'anjou'. | photoprotective function of anthocyanins along with xanthophyll cycle and antioxidant system in fruit peel was investigated in red 'anjou' vs green 'anjou' pear (pyrus communis) during fruit development and in response to short-term exposure to high light. the sun-exposed peel of red 'anjou' had higher maximum quantum yield of photosystem ii (f(v)/f(m)) than that of green 'anjou' and both the sun-exposed peel and the shaded peel of red 'anjou' had smaller decreases in f(v)/f(m) after 2-h high li ... | 2008 | 18715235 |
| hrpn of erwinia amylovora functions in the translocation of dspa/e into plant cells. | the type iii secretion system (t3ss) is required by plant pathogenic bacteria for the translocation of certain bacterial proteins to the cytoplasm of plant cells or secretion of some proteins to the apoplast. the t3ss of erwinia amylovora, which causes fire blight of pear, apple and other rosaceous plants, secretes dspa/e, which is an indispensable pathogenicity factor. several other proteins, including hrpn, a critical virulence factor, are also secreted by the t3ss. using a cyaa reporter syste ... | 2008 | 18705858 |
| evaluation of four whole-plant inoculation methods to analyze the pathogenicity of erwinia amylovora under quarantine conditions. | four methods were tested to assess the fire-blight disease response on grafted pear plants. the leaves of the plants were inoculated with erwinia amylovora suspensions by pricking with clamps, cutting with scissors, local infiltration, and painting a bacterial suspension onto the leaves with a paintbrush. the effects of the inoculation methods were studied in dose-time-response experiments carried out in climate chambers under quarantine conditions. a modified gompertz model was used to analyze ... | 2008 | 18645961 |
| a lethal form of gorham disease associated with extensive musculoskeletal pneumatosis: case report and review of the literature. | we report here the imaging findings of a rare case of a lethal form of gorham disease in a young female patient. multimodality imaging findings over 13 year-follow-up demonstrated progressive wide spread skeletal and soft tissue abnormalities with permeative osteolysis, pathological fractures and severe skeletal deformities. unusual extensive osseous and soft tissue pneumatosis was illustrated on cross-sectional studies. the progressive nature of this form of gorham disease and the subsequent co ... | 2008 | 18629462 |
| clinical and molecular delineation of the 17q21.31 microdeletion syndrome. | the chromosome 17q21.31 microdeletion syndrome is a novel genomic disorder that has originally been identified using high resolution genome analyses in patients with unexplained mental retardation. | 2008 | 18628315 |
| a rapid and effective method for rna extraction from different tissues of grapevine and other woody plants. | rna quality and integrity are critical for many studies in plant molecular biology. high-quality rna extraction from grapevine and other woody plants is problematic due to the presence of polysaccharides, polyphenolics and other compounds that bind or co-precipitate with the rna. | 2008 | 18618437 |
| macrophage immunomodulatory activity of polysaccharides isolated from opuntia polyacantha. | opuntia polyacantha (prickly pear cactus) has been used extensively for its nutritional properties; however, less is known regarding medicinal properties of opuntia tissues. in the present study, we extracted polysaccharides from o. polyacantha and used size-exclusion chromatography to fractionate the crude polysaccharides into four polysaccharide fractions (designated as opuntia polysaccharides c-i to c-iv). the average m(r) of fractions c-i through c-iv was estimated to be 733, 550, 310, and 1 ... | 2008 | 18597716 |
| factors involved in alleviating water stress by partial crop removal in pear trees. | we studied the relief of water stress associated with fruit thinning in pear (pyrus communis l.) trees during drought to determine what mechanisms, other than stomatal adjustment, were involved. combinations of control irrigation (equal to crop water use less effective rainfall) and deficit irrigation (equal to 20% of control irrigation), fruit load (unthinned and thinned to 40 fruits per tree) and root pruning (pruned and unpruned) treatments were applied to pear (cv. 'conference') trees during ... | 2008 | 18595850 |
| four species of zygophiala (schizothyriaceae, capnodiales) are associated with the sooty blotch and flyspeck complex on apple. | sooty blotch and flyspeck (sbfs) is a complex of fungi that cause late-season blemishes of apple and pear fruit that cosmetically damage the cuticle, which result in fruit that are unacceptable to consumers. previous studies reported that a single, wide-host-range species, schizothyrium pomi (presumed anamorph zygophiala jamaicensis), caused flyspeck on apple. in the present study we compared morphology and dna phylogeny (its, lsu) of 139 fungal strains isolated from flyspeck signs from 39 apple ... | 2008 | 18592899 |
| enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis. | in our previous work, an apple spermidine synthase (spds)-overexpressing transgenic european pear (pyrus communis l. 'ballad'), line no. 32 (#32), demonstrated attenuated susceptibility to stress treatment. in the current paper, changes in enzymatic and non-enzymatic antioxidant capacity of the transgenic pear (line #32) were investigated in response to nacl or mannitol stress. under non-stressed conditions (before stress treatment), spermidine (spd) contents and spds activity of line #32 were h ... | 2008 | 18586287 |
| [gorham-stout syndrome in a patient with hiv infection]. | 2008 | 18582424 | |
| control of postharvest pear diseases using rhodotorula glutinis and its effects on postharvest quality parameters. | rhodotorula glutinis was evaluated for its activity in reducing postharvest gray mold decay and blue mold decay of pear caused by botrytis cinerea and penicillium expansum respectively, and in reducing natural decay development of pear fruits, as well as its effects on postharvest quality of fruits. there was a significant negative correlation between concentrations of the yeast cells and infectivity of the pathogens. at concentrations of r. glutinis at 5x108 cfu/ml, the gray mold decay was comp ... | 2008 | 18579245 |
| identification and quantification of betalains from the fruits of 10 mexican prickly pear cultivars by high-performance liquid chromatography and electrospray ionization mass spectrometry. | qualitative and quantitative analyses of betalain pigments in 10 cultivars/lines of prickly pear (opuntia spp.) fruit grown in mexico were conducted with reverse phase high-performance liquid chromatography-diode array detection (hplc-dad) coupled with electrospray mass spectrometry (esi-ms). betacyanins and betaxanthins were identified by comparison with the uv/vis and mass spectrometric characteristics as well as the retention times of semisynthesized reference betaxanthins. data revealed that ... | 2008 | 18578538 |
| viability of lactobacillus rhamnosus r0011 in an apple-based fruit juice under simulated storage conditions at the consumer level. | lactobacillus rhamnosus r0011 was inoculated in an apple-pear-raspberry juice blend at 4.5 x 10(9) cfu/250 ml portion, and viability was followed during storage in conditions simulating consumer use. study parameters were age of the juice (2 or 4 wk), sampling size (one to three 250-ml portions), incubation temperature (between 2 and 7 degrees c), and atmosphere (aerobic compared with anaerobic). dependent variables were ph, dissolved oxygen (do) level, and viable count. the do level in the unop ... | 2008 | 18577004 |
| idiopathic calvarial thinning. | an 82-year-old diabetic female had been aware of gradually enlarging, painless scalp depressions in the bilateral parietal regions for more than 6 years. she had no history of head injury, or inflammatory or malignant disease. her family history was unremarkable for hereditary bone disease. she had diabetes mellitus which had been well controlled with orally administered drugs. blood examination showed normal renal and liver functions with normal serum calcium, magnesium, and phosphorus concentr ... | 2008 | 18574336 |
| [antitumor effect of polysaccharides from cactus pear fruit in s180-bearing mice]. | polysaccharide components of some traditional chinese medicine have certain antitumor effects and can promote immune responses. extractions from cactus pear fruit can inhibit the proliferation of cervical cancer, ovary cancer and bladder cancer cells, and suppress the growth of ovarian cancer in mice. this study was to observe the antitumor effect of polysaccharides extracted from cactus pear fruit in s180-bearing mice. | 2008 | 18570729 |
| histological features and management of a mandibular gorham disease: a case report and review of maxillofacial cases in the literature. | gorham disease (gd) is a rare osteolysis without sex, race, or age predilection, affecting bones in different regions. based on clinical, histological, and molecular features, diagnosis is difficult and required exclusion of neoplastic, inflammatory, infectious, and endocrinologic disease. etiology is still unknown. we report the case of a 36-year-old man suffering from severe progressive osteolysis located at the mandible. histology showed massive osteolysis without malignant cells. immunohisto ... | 2008 | 18567509 |
| gorham disease of the mandible: radiographic findings and radiotherapy response. | gorham disease, a rare condition of unknown etiology, is characterized histologically by bone disintegration and endothelial proliferation. we describe serial imaging findings, the treatment course, and radiotherapy response in a patient with pathologically confirmed gorham disease involving the right mandible. progressive mandibular resorption was managed initially with multiple surgical resections and reconstruction, followed by external-beam radiotherapy, which was an effective treatment meth ... | 2008 | 18561108 |
| sperm morphology and fertility of progeny-tested ai dairy bulls in sweden. | use of bull semen with high levels of sperm abnormalities, reflecting genital dysfunction, is not recommended for artificial insemination (ai) since it would most likely lead to subfertility. sperm quality, including sperm morphology, may deteriorate with increasing age of the bull thus becoming a source of concern when using older, progeny-tested ai bull sires. although a relationship between sperm morphology and fertility after ai in progeny-tested bull sires has been reported, it is yet uncle ... | 2008 | 18539320 |
| efficacy of dietary supplementation with botanicals on carbohydrate metabolism in humans. | botanical products are widely used in nutritional supplementation for promotion of health or prevention of diseases. with the high prevalence of obesity and type 2 diabetes, abnormalities in carbohydrate metabolism are common in the general population and obtaining glycemic control is important in reducing the complications of diabetes. if shown to be effective, botanical products have a unique position in potentially aiding the general public in regard to obesity and diabetes. they can be obtai ... | 2008 | 18537692 |
| efficacy of heat treatments with water and fludioxonil for postharvest control of blue and gray molds on inoculated pears and fludioxonil residues in fruit. | the residue levels of fludioxonil (flu) were determined in pear cultivars precoce di fiorano, coscia, and spadona estiva after a 2-min dip in an aqueous mixture of flu containing 300 or 100 mg/liter of active ingredient at 20 or 50 degrees c and after 12 days at 17 degrees c and 80% relative humidity (simulated shelf life conditions). the accumulation trend of flu residues was determined in 'precoce di fiorano' pears after treatments with 25, 50, 100, or 200 mg/liter of active ingredient at 20 o ... | 2008 | 18522031 |
| gorham's disease: an osseous disease of lymphangiogenesis? | gorham's disease, also known as massive osteolysis, gorham-stout disease, vanishing bone disease, or, phantom bone disease is a rare disorder of the musculoskeletal system. the disease is characterized by osteolysis in bony segments, with localized proliferation of lymphatic channels. the presence of abundant, leaky systemic lymphatic vessels is often accompanied by chylous ascites. there is no standardized treatment available for gorham's disease, and its molecular mechanisms remain unclear. fu ... | 2008 | 18519972 |
| fermentation of opuntia stricta (haw.) fruits for betalains concentration. | fermentation of juice and homogenized fruits of opuntia stricta fruits has been developed and optimized. the aim was to obtain the red food colorant betanin from prickly pear, at high concentration and low viscosity. among three strains assayed, saccharomyces cerevisiae var. bayanus awri 796 has been the optimum for this process. the optimum temperature value was found to be 35 degrees c for both sugar consumption and pigment preservation. after fermentation, biomass and residual vegetal tissue ... | 2008 | 18473472 |
| the genome of erwinia tasmaniensis strain et1/99, a non-pathogenic bacterium in the genus erwinia. | the complete genome of the bacterium erwinia tasmaniensis strain et1/99 consisting of a 3.9 mb circular chromosome and five plasmids was sequenced. strain et1/99 represents an epiphytic plant bacterium related to erwinia amylovora and e. pyrifoliae, which are responsible for the important plant diseases fire blight and asian pear shoot blight, respectively. strain et1/99 is a non-pathogenic bacterium and is thought to compete with these and other bacteria when occupying the same habitat during i ... | 2008 | 18462403 |
| gorham-stout disease of the humerus. | gorham-stout disease is characterized by local proliferation of small vascular or lymphatic channels resulting in progressive destruction and resorption of bone. the etiology and pathogenesis of the disease remains mostly unknown, despite some 175 reported cases. a case of gorham-stout disease of the humerus in a 14-year-old boy is described. the patient presented with progressive pain and deformity of the right arm. although the disease was described in different bones of the body its location ... | 2016 | 18447124 |
| disappearing bones: a case of gorham-stout disease. | 2008 | 18446639 | |
| an investigation of the psychometric properties of the mmpi-2 restructured clinical (rc) scales with mental health inpatients. | in this study, we compared the internal psychometric properties and external correlates of the mmpi-2 (butcher et al., 2001; tellegen et al., 2003) restructured clinical (rc) scales and corresponding mmpi-2 non-k-corrected clinical scales in a sample of 280 men and 264 women admitted to inpatient psychiatric units. we evaluated the internal psychometric properties of the rc and clinical scales by intercorrelation analyses and alpha coefficients. we investigated external correlates of scores from ... | 2008 | 18444120 |
| effect of seven different pollens on bio-ecological parameters of the predatory mite typhlodromus foenilis (acari: phytoseiidae). | development, immature survival, reproduction, and life table parameters of the predatory mite typhlodromus foenilis oudemans were evaluated on seven different plant pollens (almond, apple, pear, cherry, apricot, plum, and walnut). experiments were conducted in environmental chambers at 20 +/- 1 degrees c with 65 +/- 5% rh and a photoperiod of 16:8 (l:d). t. foenilis successfully completed its development on all pollen diets. female mean developmental time from egg to adult emergence ranged from ... | 2008 | 18419905 |
| three-dimensional gas exchange pathways in pome fruit characterized by synchrotron x-ray computed tomography. | our understanding of the gas exchange mechanisms in plant organs critically depends on insights in the three-dimensional (3-d) structural arrangement of cells and voids. using synchrotron radiation x-ray tomography, we obtained for the first time high-contrast 3-d absorption images of in vivo fruit tissues of high moisture content at 1.4-microm resolution and 3-d phase contrast images of cell assemblies at a resolution as low as 0.7 microm, enabling visualization of individual cell morphology, c ... | 2008 | 18417636 |
| screening preharvest/postharvest strategies to prevent fruit rot decay. | in fruit growing preharvest sprayings in the orchard are mainly applied to protect fruit from decaying. next to multisite fungicides (captan, thiram, tolylfluanid) the most commonly used products recognized for the belgium market are bellis (pyraclostrobin & boscalid) and the combination of topsin m (thiophanate-methyl) and frugico (diethofencarb). in general the spraying schedule varies depending on weather conditions (infection risk), preharvest interval of available fungicides, fruitgrower an ... | 2007 | 18396828 |
| first occurrence of strobilurin-resistant isolates of stemphylium vesicarium in an italian pear orchard. | 2007 | 18396802 | |
| measurement of glenoid bone loss: a comparison of measurement error between 45 degrees and 0 degrees bone loss models and with different posterior arthroscopy portal locations. | osteotomies at an angle of 45 degrees to the long axis of the glenoid were originally used in a cadaveric model to simulate the bone loss that can occur clinically in anterior instability of the shoulder. however, this type of glenoid defect is not consistent with the usual clinical scenario, in which bone loss occurs nearly parallel (at 0 degrees) to the long axis of the glenoid. | 2008 | 18354146 |
| feeding substrates and behaviors of western cherry fruit fly (diptera: tephritidae). | a study was conducted to determine the abundance of potential foods and the feeding substrates and behaviors of the western cherry fruit fly, rhagoletis indifferens curran (diptera: tephritidae), in 2005, 2006, and 2007 in central washington state. aphid colonies with honeydew, a presumed food source for flies, were not seen on randomly selected branches of sweet cherry trees, prunus avium l., but leaves with cherry juice, fruit that were damaged, and leaves with bird feces were commonly seen, e ... | 2008 | 18348808 |
| particle films for managing arthropod pests of apple. | a two-season study showed that a calendar-based spray program to manage arthropod pests with kaolin (60 g/liter) applied at the rate of 450 liters/ha was effective against european apple sawfly, hoplocampa testudinea (klug) (hymenoptera: tenthredinidae); white apple leafhopper, typhlocyba pomaria mcatee (homoptera: cicadellidae); apple red bug, lygidea mendax reuter (heteroptera: miridae); pear plant bug, lygocoris communis (knight) (heteroptera: miridae); and the apple rust mite, aculus schlech ... | 2008 | 18330129 |
| risk assessment of metals from consuming vegetables, fruits and rice grown on soils irrigated with waters of the ebro river in catalonia, spain. | the concentrations of arsenic (as), cadmium (cd), chromium (cr), copper (cu), mercury (hg), manganese (mn), nickel (ni), and lead (pb) were determined in samples of various edible vegetables (artichoke, cauliflower, lettuce, tomato), fruits (apple, mandarin, orange, pear) and rice grown in soils irrigated with water from the ebro river in tarragona province (catalonia, spain). although all food items were randomly acquired in various localities of the area, the local origin was always verified. ... | 2008 | 18322656 |
| diurnal variation of walnut tree volatiles and electrophysiological responses in cydia pomonella (lepidoptera: tortricidae). | in recent years, many studies have been carried out on the behavioural and electrophysiological responses of cydia pomonella (l.) to host volatile emissions, to find alternative attractants to the sex pheromone for pest monitoring. these studies have focused on apple and pear, and very little has been done on walnut. in the present work, the diurnal and seasonal variation in walnut volatile emissions and the electrophysiological response of c. pomonella have been studied. | 2008 | 18300208 |
| biological activity of ethyl (e,z)-2,4-decadienoate on different tortricid species: electrophysiological responses and field tests. | ethyl (e,z)-2,4-decadienoate (pear ester) is known to be a kairomonal attractant for both male and female codling moths, cydia pomonella (l.) (lepidoptera: tortricidae). electroantennographic (eag) studies were conducted to investigate the sensitivities of codling moth, the chestnut torticid species cydia fagiglandana (zeller), cydia splendana (hübner), and pammene fasciana l., and the green budmoth hedya nubiferana haworth to pear ester. the attractiveness of this compound to the different spec ... | 2007 | 18284724 |
| [a rare differential diagnosis of chylothorax]. | we present the case report of a 17-year-old patient with right pleural effusion and pre-existing lymphangiomas of the chest wall. thoracentesis revealed a salmon-milky effusion containing chylomicrones defining a chylothorax (with blood intermixture). a ct scan and mri showed multiple osteolysis. after excluding malignancy as well as infectious and traumatic causes of the chylothorax, we made the diagnosis of a gorham-stout syndrome. a minimal-invasive pleurectomy and decortication were done suc ... | 2008 | 18264894 |
| natural phenanthrenes and their biological activity. | the aim of this review is to survey the various naturally occurring phenanthrene compounds that have been isolated from different plants. only one review has previously been published on this topic. gorham (1989) reviewed the structures, biosynthesis, separations and spectroscopy of stilbenes and phenanthrenes. the present study furnishes an overview of the hydroxy or/and methoxy-substituted 9,10-dihydro/phenanthrenes, methylated, prenylated and other monomeric derivatives, dimeric and trimeric ... | 2008 | 18243254 |
| role of sertoli cell number and function on regulation of spermatogenesis. | testicular function is under the control of expression and repression of several genes and gene products, and many of these works through sertoli cells. the capability of sertoli cells to regulate spermatogenesis is dependent on sertoli cell functions and sertoli cell number. sertoli cell number has long been thought to be stable in adults with no proliferation of sertoli cells once adult numbers have been reached. however, adult horses do not have stable sertoli cell numbers, and new studies in ... | 2008 | 18242891 |
| consumptive coagulopathy associated with gorham syndrome and subsequent kasabach-merritt syndrome during pregnancy: a case report. | gorham and kasabach-merritt syndrome are rare diagnoses that can complicate pregnancy by increasing the risk of pelvic fracture and consumptive coagulopathy, respectively. | 2007 | 18210901 |
| efficiency assessment of a swift method to enhance substandard viability ram ejaculates. | semen availability in ram semen processing facilities is of great importance for the genetic improvement of sheep. accordingly, any method that would increase sperm viability in low viability ejaculates could be useful. in this study, the possibility of a glass beads filtration method by estimating the beads total surface provided (tsp) for adhesion of spermatozoa, was evaluated. initially, two different tsp (102 and 154 cm(2)) achieved by various sizes of beads (1500, 2000 and 3000 microm) were ... | 2008 | 18199267 |
| antioxidant content of fresh-cut pears stored in high-o2 active packages compared with conventional low-o2 active and passive modified atmosphere packaging. | the antioxidant content of fresh-cut 'flor de invierno' ( pyrus communis l.) pears dipped into a 0.75% w/v n-acetylcysteine + 0.75% w/v glutathione solution and packaged under 70 kpa of o2 atmospheres (hoa) was evaluated as an alternative to actively modified low-o2 atmosphere (loa) and passively modified atmospheres (pa). changes in color, vitamin c, individual phenolic compounds, and antioxidant activity of fresh-cut pears as well as in o2, co2, and ethylene headspace concentrations inside the ... | 2008 | 18189356 |
| efficacy of the pear ester as a monitoring tool for codling moth cydia pomonella (lepidoptera: tortricidae) in new zealand apple orchards. | the behavioural response of both sexes of codling moth, cydia pomonella to the pear-derived kairomone (ethyl (2e,4z)-2,4-decadienoate), codling moth sex pheromone (e,e-8,10-dodecadien-1-ol), and sex pheromone combined with the pear derived kairomone loaded into red rubber septum were investigated in trapping experiments in new zealand apple orchards. a range of 0.01-10.0 mg of pheromone loading in rubber septum dispensers was tested and the highest catch of males was in traps baited with 1.0 mg. ... | 2008 | 18189264 |
| comparative analysis of polyphenolic profiles and antioxidant and antimicrobial activities of tunisian pome fruit pulp and peel aqueous acetone extracts. | pome trees, apple, pear, and quince, are classified into the subfamily pomoideae, belonging to the rosaceae family. their autumnal fruits are consumed worldwide in different forms, that is, fresh or transformed into jams, jelly, juices, etc. their well-established beneficial properties to human health were found mainly related to their phenolic content. pulp and peel aqueous acetone extracts obtained from tunisian fruits at commercial maturity were comparatively evaluated for their phenolic prof ... | 2008 | 18181568 |
| sperm morphology and chromatin integrity in swedish warmblood stallions and their relationship to pregnancy rates. | artificial insemination is not as widely used in horses as in other domestic species, such as dairy cattle and pigs, partly because of the wide variation in sperm quality between stallion ejaculates and partly due to decreased fertility following the use of cooled transported spermatozoa. furthermore, predictive tests for sperm fertilising ability are lacking. the objective of the present study was to assess sperm morphology and chromatin integrity in ejaculates obtained from 11 warmblood breedi ... | 2008 | 18179691 |
| add-on mirtazapine enhances effects on cognition in schizophrenic patients under stabilized treatment with clozapine. | the development of therapeutic strategies for cognitive dysfunction remains one of the primary goals in the treatment of schizophrenia. the pharmacodynamic profile of mirtazapine, an antidepressant that enhances noradrenergic and serotonergic transmission, is based on a presynaptic alpha2 antagonism and postsynaptic 5-ht2 and 5-ht3 antagonism. mirtazapine shares some pharmacological similarities with that of clozapine. this 8-week open label trial aimed to discover whether the addition of 30 mg ... | 2007 | 18179309 |
| deletion of a 236 kb region around s 4-rnase in a stylar-part mutant s 4sm-haplotype of japanese pear. | japanese pear (pyrus pyrifolia nakai) has a gametophytic self-incompatibility (gsi) mechanism controlled by a single s-locus with multiple s-haplotypes, each of which contains separate genes that determine the allelic identity of pistil and pollen. the pistil s gene is the s-ribonuclease (s-rnase) gene, whereas good candidates for the pollen s gene are the f-box protein genes. a self-compatible (sc) cultivar, 'osa-nijisseiki', which is a bud mutant of 'nijisseiki' (s (2) s (4)), has a stylar-par ... | 2008 | 18175198 |
| [trauma and psychosis]. | since the 1990's a vast amount of research has been done on early traumatic experiences leading to serious psychopathology in adults. at the same time, several researchers started to assume a relationship between posttraumatic stress disorder (ptsd) and psychotic states. patients suffering from ptsd often experience intrusive flashback memories which at the time seem quite present, real, absorbing and uncontrollable to them. from this point of view they have quite similar experiences as psychoti ... | 2007 | 18167424 |
| effect of chitin on the antagonistic activity of cryptococcus laurentii against penicillium expansum in pear fruit. | this study was designed to evaluate the impact of chitin on the antagonistic activity of cryptococcus laurentii against the postharvest blue mold rot caused by penicillium expansum in pear fruit. the results showed that the antagonistic activity of c. laurentii obtained from the culture media of nutrient yeast dextrose broth (nydb) amended with chitin at 0.5-1.0% was improved greatly compared with the case that without chitin. the addition of chitin to nydb did not influence the growth of c. lau ... | 2008 | 18162192 |
| morphology and ultrastructure of a specialized bacterial pouch in the digestive tract of tetraponera ants (formicidae, pseudomyrmecinae). | the digestive tract in workers of some species of the pseudomyrmecine ant genus tetraponera is characterized by a conspicuous pear-shaped diverticulum at the transition between the midgut and the intestine, that so far has not been found in any other ant species. as this organ is filled with a mass of bacteria, we propose to designate it as a bacterial pouch. its distal wall is formed by a thin ectodermal epithelium, through which tens of tracheal branches penetrate into the bacterial mass that ... | 2000 | 18088931 |
| tuft cells. | tuft cells, also known as brush cells, are widespread in the hollow organs of the digestive tract including the duct system of the salivary gland and in the respiratory tract, from simple vertebrates to humans. the shape of tuft cells varies from pear-shaped, to barrel-shaped and goblet-shaped, apparently depending on the plane of section. the most characteristic morphological features of tuft cells are their long and blunt microvilli, which have prominent rootlets, and a well developed tubulove ... | 2007 | 18062147 |
| characterisation of secreted polysaccharides and (glyco)proteins from suspension cultures of pyrus communis. | high molecular weight material recovered from the culture filtrate of cell suspension cultured pyrus communis was composed of 81% carbohydrate, 13% protein and 5% inorganic material. this material was separated into three fractions (one neutral (fraction a) and two acidic (fractions b and c)), by anion-exchange chromatography on deae-sepharose cl-6b using a gradient of imidazole-hcl at ph 7.0. the monosaccharide and linkage composition of each fraction was determined after carboxyl reduction of ... | 2008 | 18037144 |
| carotenoids and color of fruit juice and milk beverage mixtures. | seventeen commercially available, "ready to drink" fortified beverages consisting of mixtures of fruit juices and milk were analyzed to evaluate their carotenoid profile (including their z/e stereoisomers) and color during their commercial shelf life. lightness (l*) was found to be correlated with the content of milk in the mixtures (r= 0.649) whereas red-yellow colors were correlated with the contents of alpha-carotene, beta-cryptoxanthin, and beta-carotene supplied by the fruit. the beverages ... | 2007 | 18034704 |
| formation of zinc-chlorophyll-derivative complexes in thermally processed green pears (pyrus communis l.). | the formation of zinc-chlorophyll-derivative complexes was investigated in peels-on green d'anjou pears when subjected to blanching in zinc ion solution (1300, 2600, and 0 ppm) at 94 degrees c for 6, 12, or 18 min and then canning at 94 degrees c for 20 min. the peels removed from the pears were freeze-dried and ground into powders in liquid nitrogen for pigment extraction using ethyl ether. the visual absorption of the extracts was measured using a spectrophotometer along with identification an ... | 2007 | 17995638 |
| positive interaction of a feeding attractant and a host kairomone for trapping the codling moth, cydia pomonella (l.). | codling moths are attracted to acetic acid and to ethyl-(e,z)-2,4-decadienoate, the pear ester, when presented individually. the attraction to acetic acid is thought to be a food finding behavior, whereas the pear odorant, ethyl-(e,z)-2,4-decadienoate, may be a host kairomone. we found, in a flight tunnel study, that more male and female codling moths were captured in traps when the compounds were presented together compared to tested separately. the combination of odorants provides a stronger l ... | 2007 | 17992562 |
| purification and characterization of a non-s-rnase and s-rnases from styles of japanese pear (pyrus pyrifolia). | in this study we biochemically characterized stylar ribonucleases (rnases) of japanese pear (pyrus pyrifolia), which exhibits s-rnase-based gametophytic self-incompatibility. we separated the rnase fractions ns-1, ns-2, and ns-3 from stylar extracts of the cultivar nijisseiki (s(2)s(4)). the rnase in each fraction was purified to homogeneity through a series of chromatographic steps. chemical analysis of the proteins revealed that the basic rnases in the ns-2 and ns-3 fractions were the s(4)- an ... | 2007 | 17988882 |
| a library of linear undecapeptides with bactericidal activity against phytopathogenic bacteria. | a 125-member library of synthetic linear undecapeptides was prepared based on a previously described peptide h-k(1)klfkkilkf(10)l-nh(2) (bp76) that inhibited in vitro growth of the plant pathogenic bacteria erwinia amylovora, xanthomonas axonopodis pv. vesicatoria, and pseudomonas syringae pv. syringae at low micromolar concentrations. peptides were designed using a combinatorial chemistry approach by incorporating amino acids possessing various degrees of hydrophobicity and hydrophilicity at po ... | 2007 | 17980935 |
| isolation and identification of species of alicyclobacillus from orchard soil in the western cape, south africa. | alicyclobacilli were isolated from orchard soil collected from an apple and pear farm in elgin, western cape, south africa. morphological, biochemical and physiological characteristics of the isolates were used to presumptively classify them as belonging to the genus alicyclobacillus. strains were identified to species level by polymerase chain reaction (pcr) with genus-specific primers, and 16s ribosomal rna (rrna) gene sequencing. to our knowledge this is the first report on the isolation of a ... | 2008 | 17938854 |
| the role of luxs in the fire blight pathogen erwinia amylovora is limited to metabolism and does not involve quorum sensing. | erwinia amylovora is a gram-negative phytopathogen that causes fire blight of pome fruit and related members of the family rosaceae. we sequenced the putative autoinducer-2 (ai-2) synthase gene luxs from e. amylovora. diversity analysis indicated that this gene is extremely conserved among e. amylovora strains. quorum sensing mediated by luxs has been implicated in coordinated gene expression, growth, and virulence in other enterobacteria; however, our evidence suggests this is not the function ... | 2007 | 17918630 |
| lymphatics and bone. | there is controversy regarding whether lymphatic vessels are present or absent in bone. although lymphangiomas have been described in bone, lymphatic vessels have not been identified morphologically with certainty in any other benign or malignant bone tumors or in normal human bone. in this study, we determined by immunohistochemistry, using 2 specific lymphatic endothelial cell markers, lyve-1 and podoplanin, whether lymphatics are present in normal bone and a wide range of primary and secondar ... | 2008 | 17904616 |
| genetic mapping of genes for susceptibility to black spot disease in japanese pears. | black spot disease, which is caused by the japanese pear pathotype of alternaria alternata (fr.) keissler, is one of the most harmful diseases in japanese pear cultivation. we identified the exact positions and linkage groups (lgs) of the genes for susceptibility to black spot in the japanese pear (pyrus pyrifolia nakai) cultivars 'osa nijisseiki' (gene ani) and 'nansui' (gene ana). segregation of susceptibility and resistance fitted the expected ratio of 1:1 in progeny of 'nansui' but showed a ... | 2007 | 17893733 |
| [effects of exogenous nitric oxide donor on lipid peroxidation of pear leaves infected by physalosproa pirico nose]. | this paper studied the effects of 0.3 mmol x l(-1) snp on the antioxidant enzymes and reactive oxygen in pear leaves infected by physalosproa piricola nose. the results showed that compared with ck1 (spraying leaves with water and infecting with p. piricola nose), 0.3 mmol x l(-1) snp could increase the activities of leaf pod, sod, cat and ppo and the contents of leaf asa, gsh and proline by 39.41%, 21.11%, 41.61%, 13.04%, 44.45%, 48.32% and 33.80%, while decrease the contents of leaf mda and h2 ... | 2007 | 17886652 |
| microbial control of insect pests in temperate orchard systems: potential for incorporation into ipm. | because of their selectivity and safety, microbial control agents (mcas) appear to be ready-made components of integrated pest management (ipm) systems that do not pose a threat to applicators or the environment and allow other natural enemies to function. control of several orchard pest insects using mcas, including viruses, bacillus thuringiensis, fungi, and entomopathogenic nematodes (epns), have been demonstrated in apple, pear, stone fruits, citrus, and several nut crops. b. thuringiensis i ... | 2008 | 17803454 |
| the perfect stage of leaf-spot of pear and quince. | 1909 | 17777277 | |
| host-specific phytotoxic polysaccharide from apple tissue infected by erwinia amylovora. | a toxin isolated from apple fruit tissue infected by erwinia amylovora is 98 percent galactose in polymeric form, 0.375 percent protein, and has an average molecular weight of approximately 165,000. young shoots of rosaceous, but not nonrosaceous, species wilt in a manner characteristic of the disease when placed in toxin solutions with concentrations as low as 10 micrograms per milliliter. varieties of apple and pear susceptible to erwinia amylovora wilt in 1 to 3 hours, whereas resistant varie ... | 1974 | 17738972 |
| antagonistic action of harpin proteins: hrpwea from erwinia amylovora suppresses hrpnea-induced cell death in arabidopsis thaliana. | harpins are proteins secreted by the type-three secretion system of phytopathogenic bacteria. they are known to induce a hypersensitive response (hr) in non-host plant leaf tissue. erwinia amylovora, the fire blight pathogen of pear and apple trees, secretes two different harpins, hrpnea and hrpwea. in the present study, we showed that an erwinia amylovora hrpwea mutant induces stronger electrolyte leakages in arabidopsis thaliana foliar disks than the wild-type strain, thus suggesting that hrpw ... | 2007 | 17726062 |
| mechanisms of antagonism of pseudomonas fluorescens eps62e against erwinia amylovora, the causal agent of fire blight. | pseudomonas fluorescens eps62e was selected during a screening procedure for its high efficacy in controlling infections by erwinia amylovora, the causal agent of fire blight disease, on different plant materials. in field trials carried out in pear trees during bloom, eps62e colonized flowers until the carrying capacity, providing a moderate efficacy of fire-blight control. the putative mechanisms of eps62e antagonism against e. amylovora were studied. eps62e did not produce antimicrobial compo ... | 2007 | 17661291 |
| evaluation of different growth media for the recovery of the species of alicyclobacillus. | five different isolation media, namely potato dextrose agar (pda), orange serum agar (osa), k agar, yeast-starch-glucose agar and bacillus acidocaldarius medium were evaluated for the recovery of alicyclobacillus spp. from inoculated diluted and undiluted fruit-juice concentrates. | 2007 | 17651223 |
| evaluation of the pear ester kairomone as a formulation additive for the granulovirus of codling moth (lepidoptera: tortricidae) in pome fruit. | (e,z) -2,4-decadienoate (pear ester) is a larval kairomone for the codling moth, cydia pomonella (l.) (lepidoptera: tortricidae). orchard studies were conducted in 2005 and 2006 in apple, malus domestica borkhausen, and pear, pyrus communis l., to evaluate a 5% active ingredient (ai), microencapsulated formulation of pear ester (pe-mec) as an insecticidal additive for the codling moth granulovirus (cpgv). although cpgv applied at 5-15-d intervals at commercial rates (2.2 x 10(12)-10(13) granules ... | 2007 | 17598528 |
| comparison of specificity and sensitivity of immunochemical and molecular techniques for reliable detection of erwinia amylovora. | erwinia amylovora [(burrill) winslow et al.] (ea), the causal agent of fire blight, was detected in plant samples and pure bacterial cultures by means of pcr, ifas and elisa. polyclonal antibodies of neogen europe ltd. were used for ifas and pta-elisa and laboratory-generated primers eaf72 and ear560 for pcr. using the biolog system and an immature pear fruit assay, identities of all ea strains were confirmed as the fire blight bacterium. in assays of pure ea cultures, pta-elisa, and both ifas a ... | 2007 | 17575916 |
| proteomic analysis of core breakdown disorder in conference pears (pyrus communis l.). | 2-de was applied to study core breakdown disorder in controlled atmosphere stored 'conference' pears. this physiological disorder is characterized by internal browning of the fruit tissue and the development of cavities. suitable protein phenol extraction/ammonium acetate-methanol precipitation and 2-de protocols for a wide ph range were established for pear tissue. the protein expression profiles of healthy, sound (intact tissue of pears with core breakdown) and brown tissue were analyzed with ... | 2007 | 17566975 |