Publications

isolation and characterization of naturally occurring hairpin structures in single-stranded dna of coliphage m13.		1977	603641
studies on dna unwinding. proton and phosphorus nuclear-magnetic-resonance studies of gene v protein from bacteriophage m13, interacting with d(pc-g-c-g).	the interaction of gene v protein from bacteriophage m13 with the self-complementary tetranucleotide d(pc-g-c-g) was studied by 1h and 31p nuclear magnetic resonance. it is shown, using the hydrogen-bonded proton resonances of the watson-crick base pairs as a probe, that the protein is able to unwind the small double-helical fragment even at 0 degrees c. binding of the tetranucleotide causes changes in the aromatic part of the 1h nmr spectrum of the complex, suggesting that aromatic residues, mo ...	1977	598376
molecular basis of the am8h1 lesion in bacteriophage m13.		1979	462808
a fast and simple method for sequencing dna cloned in the single-stranded bacteriophage m13.		1979	448736
identification of two new capsid proteins in bacteriophage m13.		1979	387445
nucleotide sequence of the genes iii, vi and i of bacteriophage m13.	a dna region of 2750 base pairs encompassing the genes iii, vi and i of bacteriophage m13 has been sequenced by the maxam-gilbert procedure. by establishing the nucleotide changes introduced by several amber mutations, the coding region and the regulatory signals of each gene have been deduced. the genes appear to span 1275 base pairs (gene iii; mol.wt. 44,748) 339 base pairs (gene vi; mol.wt. 12,264) and 1047 base pairs (gene i; mol.wt. 39,500). their separating non-codogenic regions are extrem ...	1979	379830
construction of an m13 histidine-transducing phage: a single-stranded cloning vehicle with one ecori site.	in order to create a ready source of single-stranded dna for dna sequence determination by the dideoxy chain-termination method, the promoter-proximal part of the histidine operon, the hisogd region of salmonella typhimurium, was cloned onto the single-stranded phage m13. both orientations of the his dna were cloned to supply dna template for sequencing of each strand. insertion was achieved at an haeiii site in the intergenic region (ir) of m13, and a single ecori site was purposely regenerated ...	1979	376403
soluble precursor of an integral membrane protein: synthesis of procoat protein in escherichia coli infected with bacteriophage m13.	prior to virus assembly, the major coat protein of coliphage m13 is an integral protein of the host cytoplasmic membrane. coat protein synthesized in vitro is initially made with an nh2-terminal "leader peptide" of 23 amino acids and is termed "procoat." we now report that procoat is a biosynthetic precursor of coat protein in vivo. conversion of procoat to coat occurs within 30 sec in cells infected with wild-type virus. this proteolytic processing is delayed in cells infected by m13 mutants (i ...	1979	375229
nucleotide sequence of the recognition site of the b-specific restriction modification system in e. coli.	two sb mutations in the genome of bacteriophage fd were located by sequence analysis in the fd sequence at positions 971 and 6341. base changes at or close to these positions in phage m13 and in phage fl am 124 also correlate with a loss of sensitivity to b restriction. from the sequence homology between the sequences at the two sb sites the recognition signal for the e. coli b restriction/modification enzzyme is predicted to be: 5' tga---8n---tgct 3' 3' act---8n---acga 5'.	1979	374993
translational and post-translational cleavage of m13 procoat protein: extracts of both the cytoplasmic and outer membranes of escherichia coli contain leader peptidase activity.	the coat protein of coliphage m13 is an integral protein of the host cytoplasmic membrane at all stages of the infectious cycle. both in in vivo and dna-directed in vitro synthesis, it is initially made with an nh2-terminal "leader peptide" of 23 amino acids and is termed procoat. we now report that leader peptidase, and activity which removes the leader peptide and converts procoat to coat, is found in both the inner (cytoplasmic) and outer membrane of escherichia coli. however, only cytoplasmi ...	1979	370824
nucleotide sequence of gene vii and of a hypothetical gene (ix) in bacteriophage m13.	a dna fragment containing gene vii of bacteriophage m13 has been transcribed and the nucleotide sequence of this 169-nucleotides long transcript was determined by rna sequencing methods. additionally, the nucleotide sequence of this gene and parts of its neighbouring genes v and viii has been determined by the dimethylsulphate-hydrazine technique. the reading frame of gene vii has been established by determining the nucleotide changes occurring in the transcripts of two amber mutants of this gen ...	1978	370777
replication of bacteriophage m13. xiv. differential inhibition of the replication of m13 and m13 miniphage in a mutant of escherichia coli defective in the 5' leads to 3' exonuclease associated with dna polymerase i.	previous studies have shown that m13 single-strand synthesis is inhibited at nonpermissive temperature in escherichia coli polaexl, a temperature-sensitive mutant defective in the 5' leads to 3' exonuclease activity of polymerase i (t.-c. chen and d. s. ray, j. mol. biol. 106:589-604, 1976). under these conditions the formation of covalently closed replicative form (rf) molecules is greatly reduced, and miniature forms of rf accumulate. we show here that the accumulation of mini-rfs is the conse ...	1978	366176
a photo-cidnp study of the interaction of oligonucleotides with gene-5 protein of bacteriophage m13.	it is shown that photo-cidnp effects (cidnp, chemically induced dynamic nuclear polarization) can be generated in the 360-mhz proton nmr spectrum of gene-5 protein from bacteriophage m13. this technique is used to determine the number of tyrosyl residues at the surface of the protein and to assign the resonances from the 3,5-ring protons of these residues. the dna-binding site of the protein is investigated by formation of complexes with oligonucleotides. complex formation leads to shifting and/ ...	1978	364473
insertion of the tn3 transposon into the genome of the single-stranded dna phage m13.	the transposable genetic element tn3, which carries an ampicillin (ap) resistance determinant, has been translocated from a cole1-apr plasmid, rsf2124, to the genome of the filamentous single-stranded dna phage m13. the site orientation of the inserted element has been determined for one such phage, m13::tn3-15. the insertion is within the intergenic space separating genes 2 and 4 and containing both the viral strand and complementary strand origins. the lengths of both the filamentous phage and ...	1978	363518
expression of bacteriophage m13 dna in vivo. i. synthesis of phage-specific rna and protein in minicells.	it is demonstrated that after infection of the appropriate minicell-producing strain of escherichia coli with the filamentous bacteriophage m13, its replicative form dna is segregated into minicells. consequently these minicells have acquired the capability to direct the synthesis of phage-specific rna and protein. comparision of the electrophoretic mobilities of phage-specific rna species made in vitro with those made in m13 replicative form dna harbouring minicells, have indicated that almost ...	1978	363158
comparison of specific binding sites for escherichia coli rna polymerase with naturally occurring hairpin regions in single-stranded dna of coliphage m13.		1978	353054
function of phospholipids in escherichia coli. characterization of a mutant deficient in cardiolipin synthesis.	screening of a collection of temperature-sensitive mutants of escherichia coli for defects in phospholipid metabolism led to the isolation of a mutant deficient in cardiolipin synthesis. the defective gene, named cls, is closely linked to the trp marker and maps at about minute 27 on the e. coli chromosome. after transfer of cls to a defined genetic background by transduction, the mutant has the following properties as compared to an isogenic wild type. exponentially growing cells show a reducti ...	1978	353047
replication of bacteriophage m13. xii. in vivo cross-linking of a phage-specific dna binding protein to the single-stranded dna of bacteriophage m13 by ultraviolet irradiation.		1977	336896
the role of dna polymerase i-associated 5'-exonuclease in replication of coliphage m13 replicative-form dna.		1977	334178
effect of escherichia coli dna binding protein on the transcription of single-stranded phage m13 dna by escherichia coli rna polymerase.		1977	334166
filamentous coliphage m13 as a cloning vehicle: insertion of a hindii fragment of the lac regulatory region in m13 replicative form in vitro.	a hindii restriction fragment comprising the escherichia coli lac regulatory region and the genetic information for the alpha peptide of beta-galactosidase (beta-d-galactosidegalactohydrolase, ec. 3.2.1.23) has been inserted into 1 of the 10 bsu i cleavage sites of m13 by blunt end ligation. a stable hybrid phage was isolated and identified by its ability to complement the lac alpha function. further characterization of the hybrid phage includes retransformation studies, agarose gel electrophore ...	1977	333444
base-unpaired regions in supercoiled replicative form dna of coliphage m13.	superhelical covalently closed circular replicative form dna (rf i) of coliphage m13 appears as a relaxed molecule that has a base-unpaired region in the form of a bubble (100 to 200 base pairs long) seen in electron micrographs when spread in the presence of formaldehyde and formamide or after pretreatment with glyoxal. s1 endonuclease, specific for single-stranded dna, converts superhelical m13 rf i dna, but not nonsuperhelical m13 rf i to a significant extent, into unit-length linear molecule ...	1977	328505
inhibition of bacteriophage m13 and phix174 duplex dna replication and single-strand synthesis in temperature-sensitive dnaz mutants of escherichia coli.	a functional dnaz product, known to be essential for host dna polymerization and for the synthesis of m13 and phix174 parental replicative-form (rf) dna, is required also for rf replication and single-strand synthesis by both of these phages. all three stages of m13 and phix174dna replication (parental rf formation, rf replication, and single-strand synthesis) are inhibited in dnazts mutants at elevated temperatures. in addition, the thermolabile step in m13 parental rf formation appears to occu ...	1977	323515
stability of the carrier state in bacteriophage m13-infected cells.	bacteriophage m13-infected carrier cells were shown to be unstable to prolonged growth under all conditions. carrier hfr cells were transferred in dilute culture (10(3) to 10(4)/ml), where reinfection was impossible and the physiology of the cell was minimally altered. after an initial period of about 10 generations, during which all cells in the culture remained infected, there was exponential decay in the proportion of infected cells in the culture. uninfected cells that appeared were m13 sens ...	1977	321804
photoreactive labeling of m13 coat protein in model membranes by use of a glycolipid probe.	coliphage m13 coat protein in synthetic bilayer membranes was labeled by use of 12-(4-azide-2-nitrophenoxy)stearoyl[1-14c]glucosamine, a photoreactive glycolipid probe that spontaneously inserts into membranes. in this model system, the probe preferentially labeled the proteins over the lipids. experiments designed to test the probe's restriction to integral membrane proteins revealed that extrinsic proteins as well as external peptide fragments of integral membrane proteins were not accessible ...	1979	293655
nucleotide sequence of the origin for bacteriophage m13 dna replication.		1979	289455
synthesis of phage m13 coat protein and its assembly into membranes in vitro.	the coat protein (gene 8 product) of coliphage m1o is an integral protein of the host cell membrane at all stages of virus infection. this protein, when made in a cell-free reaction, has been shown by others to have an additional nh2-terminal peptide region and is referred to as "procoat." it is initially not membrane-bound but, upon exposure to escherichia coli membrane vesicles or to liposomes prepared from e. coli lipids, it assembles into the bilayer in an integral fashion. much of this prot ...	1978	273906
structure of nascent replicative form dna of coliphage m13.	nascent replicative form type ii (rfii) dna of coliphage m13 synthesized in an escherichia coli mutant deficient in the 5' leads to 3' exonuclease associated uith dna polymerase i contains ribonucleotides that are retained in the covalently closed rfi dna sealed in vitro by the joint action of t5 phage dna polymerase and t4 phage dna ligase. these rfi molecules are labile to alkali and rnase h, unlike the rfi produced either in vivo or from rfii with e. coli dna polymerase i and e. coli dna liga ...	1978	272630
cloning of a functional replication origin of phage g4 into the genome of phage m13.		1979	231682
fractionation of membrane vesicles from coliphage m13-infected escherichia coli.	membrane vesicles were prepared by osmotic lysis of spheroplasts from m13-infected escherichia coli. reduced nicotinamide adenine dinucleotide (nadh) oxidase (reduced nad: oxidoreductase, ec 1.6.99.3) and mg2+-ca2+-activated adenosine triphosphatase (atp phosphohydrolase, ec 3.6.1.3), which are normally localized to the inner surface of the cytoplasmic membrane, were 50% acceesible to their polar substrates in these vesicles. the major coat protein of coliphage m13 is also bound to the cytoplasm ...	1976	132427
asymmetric orientation of a phage coat protein in cytoplasmic membrane of escherichia coli.	the coat protein of a filamentous phage (m13) enters the cytoplasmic membrane from two directions: from the outside upon infection and from the cell interior late in the viral life cycle prior to phage assembly and extrusion. binding of 125i-labeled anti-coat protein antibody to spheroplasts or to inverted vesicles was used to assay the orientation of coat protein in the membrane. both parental and newly synthesized coat protein were found to be exposed on the outer surface of the cytoplasmic me ...	1975	54916
membrane-associated assembly of m13 phage in extracts of virus-infected escherichia coli.	assembly of coliphage m13 is known to occur as the viral dna crosses the cytoplasmic membrane, shedding its virus-coded dna unwinding protein and acquiring from the membrane approximately 2400 copies of the major coat protein. conditions are described in which extracts of m13-infected e. coli and membranes prepared from such extracts will support virus assembly at a rate equivalent to that of intact cells. extracts prepared from cells infected with temperature-sensitive m13 mutants in genes 1, 3 ...	1977	15248