Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| transcriptional control of the equine herpesvirus 1 immediate early gene. | transient expression assays measuring induction of an equine herpesvirus 1 (ehv-1) immediate early (ie) promoter construct, pie beta gal, were used to examine trans-induction of the ie gene (ir1) promoter by superinfection with intact ehv-1 (kentucky a strain), uv-inactivated ehv-1, or ehv-1 stocks highly enriched for defective interfering particles (dips), and by cotransfection with plasmids containing ehv-1 dna fragments. our results confirm reports by others in that the ie promoter can be ind ... | 1993 | 8249301 |
| [recent information about the etiopathogenesis of paretic-paralytic forms of herpesvirus infection in horses]. | from spring 1990 to summer 1991 we investigated 21 horses with clinical symptoms of ehv-infection by means of serological and virological methods including dna-hybridization to identify the causative agents. the results indicated that, as already reported by us, ehv4 may also cause the paralytic form of the infection. the possibility of double infection with ehv4 and ehv1 cannot be excluded. in 3 out of 21 affected horses we could investigate brain tissue and/or spinal fluid by dotblot hybridiza ... | 1993 | 8248905 |
| interactions between equine herpesvirus type 1 and equine herpesvirus type 4: t cell responses in a murine infection model. | interactions involving the immune responses to equine herpesvirus types 1 and 4 (ehv-1 and ehv-4) were studied in a murine infection model. when mice were inoculated intranasally with ehv-1, virus replication occurred in the respiratory tract and clinical signs were produced. in contrast, mice that were similarly inoculated with ehv-4 produced no evidence of virus replication and showed no clinical signs. when mice that had been inoculated with live ehv-4 were challenged 1 month later with ehv-1 ... | 1993 | 8245851 |
| dna sequence of a gene cluster in the equine herpesvirus-4 genome which contains a newly identified herpesvirus gene encoding a membrane protein. | complete dna sequences for the equine herpesvirus-4 (ehv-4) genes analogous to equine herpesvirus-1 (ehv-1) genes 8, 9, 10, and 11, varicella zoster virus (vzv) genes 7, 8, 9 a, and 9, and herpes simplex virus type 1 (hsv-1) genes ul51, ul50, ul49a, and ul49 are presented. the ehv-4 gene corresponding to ehv-1 gene 10/vzv gene 9a/hsv-1 ul49a is of particular interest in that it is a newly identified herpesvirus gene whose product demonstrates features characteristic of membrane-inserted proteins ... | 1993 | 8240007 |
| effect of a booster vaccination against influenza and equine herpes virus on cardio-respiratory adjustments to strenuous exercise and training in thoroughbred horses. | this study was conducted in order to assess whether exercise- and training-induced cardio-respiratory adjustments are modified during the 10-day period which follows a booster vaccination with an oily adjuvanted inactivated vaccine against influenza and equine herpesvirus-1 (equiffa). nine healthy vaccinated thoroughbred horses were used. six were revaccinated and three were kept as control. all the horses completed a standardised exercise test (set) that was repeated 4 times, i.e. 10 (set1) and ... | 1993 | 8237183 |
| detection and identification of equine herpesvirus-1 and -4 by polymerase chain reaction. | a rapid method for detection and identification of equine herpesvirus-1 and -4 (ehv-1 and ehv-4) was developed using polymerase chain reaction (pcr). primers for pcr were designed from aligned nucleotide sequences of glycoprotein b genes of ehv-1 and ehv-4 to amplify specific regions for ehv-1 or ehv-4 or a common region of both viruses. by using type specific primer mixture, amplified fragments were identified as ehv-1 or ehv-4 in a one-step reaction. we have applied this technique on specimens ... | 1993 | 8236780 |
| a dot immunobinding assay in comparison with the gel diffusion test for the detection of equine herpesvirus-1 antigen from field samples. | the authors describe a rapid and simple dot immunobinding assay (dia) for detection and identification of equine herpesvirus-1 antigen in field samples from cases of abortion, stillbirth, perinatal foal mortality and paralysis. the assay employs a nitrocellulose membrane to which antigen is adsorbed as a dot. antigen is identified as a coloured dot using a procedure based on the principle of enzyme-linked immunosorbent assay (elisa). in all, 61 samples were tested by dia and the test was compare ... | 1993 | 8219342 |
| identification of an infectious laryngotracheitis virus gene encoding an immunogenic protein with a predicted m(r) of 32 kilodaltons. | the nucleotide sequence of an infectious laryngotracheitis virus (iltv) gene which maps immediately upstream from the glycoprotein 60 (gp60) gene was determined. the gene, designated p32, encodes a predicted polypeptide of 298 amino acids with an estimated m(r) of 32,000 daltons. the predicted protein sequence has four potential n-glycosylation sites and a signal sequence at the n-terminal region. amino acid residues in the nh2-terminal region of the p32 protein exhibit similarity to glycoprotei ... | 1993 | 8212855 |
| immunological characterization of the feline herpesvirus-1 glycoprotein b and analysis of its deduced amino acid sequence. | feline herpesvirus 1 (fhv-1) is an important viral pathogen of cats. like other alphaherpesviruses, fhv-1 contains a hsv-1 glycoprotein b (gb) homolog. in this study, monospecific antisera to hsv-1 gb reacted with three fhv-1 proteins (100, 64, and 58 kda) present in virion lysates by immunoprecipitation and immunoblot analyses. reduced stringency hybridization experiments using a hsv-1 gb probe localized the fhv-1 gb gene to a 9.6-kb sa/l fragment in the unique long region of the genome. northe ... | 1993 | 8212548 |
| identification and characterization of the icp22 protein of equine herpesvirus 1. | the equine herpesvirus 1 (ehv-1) homolog of herpes simplex virus type 1 icp22 is differently expressed from the fourth open reading frame of the inverted repeat (ir4) as a 1.4-kb early mrna and a 1.7-kb late mrna which are 3' coterminal (v. r. holden, r. r. yalamanchili, r. n. harty, and d. j. o'callaghan, j. virol. 66:664-673, 1992). to extend the characterization of ir4 at the protein level, the synthesis and intracellular localization of the ir4 protein were investigated. antiserum raised aga ... | 1994 | 8207808 |
| unusual phosphorylation sequence in the gpiv (gi) component of the varicella-zoster virus gpi-gpiv glycoprotein complex (vzv ge-gi complex). | varicella-zoster virus (vzv) glycoprotein gpiv, to be renamed vzv gi, forms a heterodimer with glycoprotein gpi (ge) which functions as an fc receptor in virus-infected cells. like vzv gpi (ge), this viral glycoprotein is phosphorylated in cell culture during biosynthesis. in this report, we investigated the nature and specificity of the phosphorylation event involving vzv gpiv (gi). phosphoamino acid analysis indicated that gpiv (gi) was modified mainly on serine residues. to identify the preci ... | 1994 | 8207795 |
| sequences of the ribonucleotide reductase-encoding genes of equine herpesvirus 4. | the equine herpesvirus 4 (ehv-4) genes encoding the two subunits of the enzyme ribonucleotide reductase (rr) were cloned and their nucleotide (nt) sequences determined. the large subunit (rr1) is predicted to comprise 789 amino acids (aa), which compares with lengths of 790, 775 and 1137 aa for the rr1 proteins encoded by equine herpesvirus 1 (ehv-1) gene 21, varicella zoster virus (vzv) gene 19 and herpes simplex virus type 1 (hsv-1) ul39, respectively. in common with vzv rr1, the ehv-4 rr1 pro ... | 1994 | 8206376 |
| helicase-primase complex of herpes simplex virus type 1: a mutation in the ul52 subunit abolishes primase activity. | the ul52 gene product of herpes simplex virus type 1 (hsv-1) comprises one subunit of a 3-protein helicase-primase complex that is essential for replication of viral dna. the functions of the individual subunits of the complex are not known with certainty, although it is clear that the ul8 subunit is not required for either helicase or primase activity. examination of the predicted amino acid sequence of the ul5 gene reveals the existence of conserved helicase motifs; it seems likely, therefore, ... | 1994 | 8189507 |
| cloning and expression of an equine herpesvirus 1 origin-binding protein. | equine herpesvirus 1 (ehv-1) is an important pathogen of horses and is closely related to several important human pathogens, herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) and varicella-zoster virus. the ehv-1 genome contains open reading frames similar in sequence to the hsv-1 replication genes. pcr was used to clone ehv-1 gene 53, which is similar in sequence to the hsv-1 ul9 gene. the gene 53 product has regions of striking similarity to the hsv-1 ul9 and vzv gene 51 products. in vitro ... | 1994 | 8189505 |
| identification of a dimerization domain in the c-terminal segment of the ie110 transactivator protein from herpes simplex virus. | the 775-amino-acid ie110 (or icp0) phosphoprotein of herpes simplex virus (hsv) functions as an accessory transcription factor during the lytic cycle and plays a critical role in reactivation from latent infection. by immunofluorescence analysis, ie110 localizes in a novel pattern consisting of several dozen spherical punctate granules in the nuclei of dna-transfected cells. we constructed a hybrid version of ie110 that contained an epitope-tagged domain from the n terminus of the hsv ie175 prot ... | 1994 | 8151788 |
| the open reading frames 1, 2, 71, and 75 are nonessential for the replication of equine herpesvirus type 1 in vitro. | equine herpesvirus type 1 (ehv-1) strain ab4 has five genes, 1, 2, 67, 71, and 75, which have no homologues in any of the herpesviruses sequenced to date; i.e., they are unique to ehv-1. the functions of these unique genes are not known. to study their role in the virus life cycle, we have constructed four independent mutants in which the majority of the coding sequences of genes 1, 2, 71, and 75 has been deleted and replaced by the escherichia coli lacz gene. mutant ed1 has a deletion of 508 bp ... | 1994 | 8122373 |
| the complete dna sequence and the genetic organization of the short unique region (us) of the bovine herpesvirus type 1 (st strain). | the dna sequence of bovine herpesvirus type 1 (bhv-1) st strain (bhv-1.2 subtype) entire unique short (us) region and part of adjacent flanking sequences of the inverted repeats was determined. the bhv-1 st us region is 9745 bp in size and has a 70.5% g + c content. eight potential open reading frames (orfs) longer than 100 amino acids and designated orf1 to orf8 were identified on this sequence. seven of these had counterparts in the us of herpes simplex type 1 (hsv-1), pseudorabies virus (prv) ... | 1994 | 8122370 |
| the equine herpesvirus type 1 glycoprotein homologous to herpes simplex virus type 1 glycoprotein m is a major constituent of the virus particle. | glycoprotein 45 is a major envelope glycoprotein of equine herpesvirus type 1. the gene encoding this protein is located between map units 0.615 and 0.636 on the virus genome and evidence has suggested that it is encoded by gene 52, one of four genes within this region. using pcr we have amplified gene 52 and subsequently cloned it into a mammalian expression vector under the control of the human cytomegalovirus immediate early gene promoter. the gene was expressed in cos-7 cells and its product ... | 1994 | 8113768 |
| molecular evolution of herpesviruses: genomic and protein sequence comparisons. | phylogenetic reconstruction of herpesvirus evolution is generally founded on amino acid sequence comparisons of specific proteins. these are relevant to the evolution of the specific gene (or set of genes), but the resulting phylogeny may vary depending on the particular sequence chosen for analysis (or comparison). in the first part of this report, we compare 13 herpesvirus genomes by using a new multidimensional methodology based on distance measures and partial orderings of dinucleotide relat ... | 1994 | 8107249 |
| a nested pcr for the detection and differentiation of ehv-1 and ehv-4. | the nested pcr method was applied for the detection and direct differentiation of equine herpesvirus type 1 (ehv-1) and type 4 (ehv-4). primer pairs were chosen from the glycoprotein b (gb) coding region of each serotype. the outer and inner ehv-1 primer pairs were type-specific, whereas the outer ehv-4 primer pair amplified ehv-1 and ehv-4 dna and was therefore suitable for the detection of both virus types in a single sample. however, the nested ehv-4 primer pair was type-specific. the advanta ... | 1993 | 8106604 |
| cross-hybridization of equid herpesvirus-2 (ehv-2) and herpes simplex virus-1 (hsv-1) genes to equid herpesvirus-1 (ehv-1). | in contrast to previous findings, the ab4 isolate of equid herpesvirus-1 (ehv-1) was shown to share homology with the g9 isolate of equid herpesvirus-2 (ehv-2). using southern blotting and stringent hybridization conditions, a significant proportion of this cross-hybridization was identified by the immediate-early gene-3 (ie-3) probe from herpes simplex virus-1 (hsv-1). the hsv-1 ul48 gene probe (encoding the ie gene transactivating protein vmw65, which is also known as alpha-tif or vp16) was us ... | 1993 | 8103247 |
| nucleotide sequence and characterization of the feline herpesvirus type 1 immediate early gene. | we report the nucleotide sequence of feline herpesvirus type 1 (fhv-1) immediate early (ie) gene encoding a homologue to the icp4 protein of herpes simplex virus type 1 and its flanking sequences. when we examined the regulatory function of the fhv-1 ie gene product by using the transient transfection assay with an effector plasmid expressing the fhv-1 ie gene in combination with chimeric various promoter-chloramphenicol acetyltransferase reporter constructs, the product was able to transactivat ... | 1994 | 8091674 |
| large and small subunits of the aujeszky's disease virus ribonucleotide reductase: nucleotide sequence and putative structure. | we determined the entire dna sequence of two adjacent open reading frames of aujeszky's disease virus encoding ribonucleotide reductase genes with the intergenic sequence of 9 bp. from the sequence analysis we deduce that orfs encode large and small subunits, with sizes of 835 and 303 amino acids, respectively. amino acid sequence comparison of adv rr2 with that of equine herpesvirus type 1, bovine herpesvirus type 1, hsv-1 and varicella zoster virus revealed that 48% of amino acids represent cl ... | 1994 | 8086454 |
| identification and initial characterization of the ir6 protein of equine herpesvirus 1. | the ir6 gene of equine herpesvirus 1 (ehv-1) is a novel gene that maps within each inverted repeat (ir), encodes a potential protein of 272 amino acids, and is expressed as a 1.2-kb rna whose synthesis begins at very early times (1.5 h) after infection and continues throughout the infection cycle (c. a. breeden, r. r. yalamanchili, c.f. colle, and d.j. o'callaghan, virology 191:649-660,1992). to identify the ir6 protein and ascertain its properties, we generated an ir6-specific polyclonal antise ... | 1994 | 8057419 |
| rapid, single-step differentiation of equid herpesviruses 1 and 4 from clinical material using the polymerase chain reaction and virus-specific primers. | sets of primers were designed which enabled specific amplification of homologous regions of the glycoprotein c and gene 76 genetic loci of equine herpesviruses 1 and 4 (ehv-1 and ehv-4). the resultant virus-specific polymerase chain reaction (pcr) products arising from each loci could be discriminated easily on the basis of size on an agarose gel, allowing rapid differentiation of the two equine herpesviruses. specificity of the amplifications were confirmed by southern hybridization and restric ... | 1994 | 8051234 |
| expression of equine herpesvirus type 1 glycoprotein gp14 in escherichia coli and in insect cells: a comparative study on protein processing and humoral immune responses. | the extracellular portion (amino acids 1 to 844) of the equine herpesvirus type 1 (ehv-1) glycoprotein gp14, the homologue of gb of herpes simplex virus, was expressed in escherichia coli and in insect cells using a recombinant baculovirus. immunoblot analysis revealed that the recombinant e. coli expressed a fusion protein of m(r) 135k which was composed of the truncated gp14 and the maltose-binding protein (mbp) provided by the vector and a 90k protein lacking the mbp moiety. both proteins wer ... | 1994 | 8046406 |
| the trigeminal ganglion is a location for equine herpesvirus 1 latency and reactivation in the horse. | four specific pathogen-free ponies were infected intranasally with equine herpesvirus 1 (ehv-1) and two were similarly infected with an ehv-1 thymidine kinase deletion mutant. the primary infections were characterized by a transient fever accompanied by virus shedding into nasal mucus and viraemia. no virus was detected in clinical specimens after 15 days post-infection. two months later a reactivation stimulus was administered to all six ponies and only the four that had been previously inocula ... | 1994 | 8046404 |
| in-situ hybridization for demonstration of equine herpesvirus type 1 dna in paraffin wax-embedded tissues and its use in horses with disseminated necrotizing myeloencephalitis. | the detection of equine herpesvirus type 1 (ehv-1) in infected cell cultures, and in tissues taken at necropsy, by the in-situ hybridization technique is described. a 4.9 kb bam hi fragment of ehv-1 vaccine strain rach was used as a probe after labelling with [alpha-32p] thymidine 5'-triphosphate ([32p]ttp) or digoxigenin-deoxyuridine 5'-triphosphate (dutp). both probes specifically detected ehv-1 dna in either cytospin or paraffin wax-embedded preparations of infected cells. the digoxigenin-lab ... | 1994 | 8040387 |
| the extreme carboxyl terminus of the equine herpesvirus 1 homolog of herpes simplex virus vp16 is essential for immediate-early gene activation. | gene 12 of equine herpesvirus 1 (ehv-1), the homolog of herpes simplex virus (hsv) vp16 (alpha tif, vmw65), was cloned into a eukaryotic expression vector by pcr and used in transactivation studies of both the ehv-1 and hsv-1 ie1 promoters. results demonstrated that the product of gene 12 is a potent transactivator of immediate-early gene expression of both viruses, which requires sequences in the upstream hsv-1 promoter for activity. mutational analysis of the gene 12 open reading frame indicat ... | 1994 | 8035487 |
| the equine herpesvirus type 1 immediate-early gene product contains an acidic transcriptional activation domain. | the equine herpesvirus type 1 (ehv-1) immediate-early (ie) gene product, an icp4 homolog, is the major regulatory protein encoded by ehv-1 during cytolytic infection. the ie gene product has been demonstrated to induce reporter gene expression directed by both homologous and heterologous viral promoters, including the ehv-1 thymidine kinase (tk) promoter, the herpes simplex virus type 1 (hsv-1) tk and icp4 promoters, and the simian virus 40 early promoter. in this report, the transcriptional act ... | 1994 | 8030239 |
| a gd homologous gene of feline herpesvirus type 1 encodes a hemagglutinin (gp60). | a gene-encoding glycoprotein d (gd) homologue of feline herpesvirus type 1 (fhv-1) was identified and sequenced. it was located within an ecori 4.3-kbp fragment in the middle of the unique short region. the primary translation product of the gd homologous gene is predicted to consist of 374 amino acids with a molecular weight (mw) of 43.2 kda. comparative analysis among gd counterparts of other herpesviruses revealed that six cysteine residues which correlate with disulfide bond structure were c ... | 1994 | 8030205 |
| characterization of immune responses to baculovirus-expressed equine herpesvirus type 1 glycoproteins d and h in a murine model. | a murine intranasal infection model for equine herpesvirus type 1 (ehv-1) was used to evaluate immune responses following immunization with insect cells infected by baculoviruses that express ehv-1 glycoproteins. baculovirus recombinant glycoprotein d (gd) and gh both induced serum antibodies to ehv-1 when measured by elisa. the gd recombinant also produced a neutralizing antibody response. protective immunity, determined by accelerated clearance of virus from the target organs in the respirator ... | 1994 | 8021602 |
| the dna sequence coding for the 5' untranslated region of herpes simplex virus type 1 icp22 mrna mediates a high level of gene expression. | the sequence coding for the 5' untranslated region (utr) of icp22 mrna of herpes simplex virus type 1 has been tested for its ability to regulate gene expression. this sequence was placed in frame with the chloramphenicol acetyltransferase (cat) coding sequence and under the control of the simian virus 40 early promoter-enhancer. under these conditions, the sequence coding for the 5'utr led to an increase of about 13-fold in cat activity, measured during transient expression. the use of mutants ... | 1994 | 8021598 |
| major histocompatibility complex class i-restricted cytotoxic t-lymphocyte responses in horses infected with equine herpesvirus 1. | an experimental system that permits sensitive and reproducible detection of equine herpesvirus 1 (ehv-1)-specific cytotoxic t-lymphocyte (ctl) activity in the horse was developed. peripheral blood mononuclear cells (pbmc) collected from immune horses were restimulated in vitro by culture with live ehv-1. cytotoxic activity against virus-infected, pokeweed mitogen-stimulated lymphoblast targets was assessed in a 4-h 51cr release assay. the optimal conditions for in vitro stimulation of equine mem ... | 1995 | 7983765 |
| prevalence of antibodies against some equine viruses in zebra (zebra burchelli) in the kruger national park, 1991-1992. | the presence of antibodies against equine encephalosis virus (eev) and equid herpesvirus 1 and 4 in zebra in the kruger national park (knp) was demonstrated. the ability of zebra to maintain immunity against eev is illustrated by the appearance of neutralizing antibodies in most zebra foals within months of losing their maternal immunity. this occurs in every month of the year, even in winter. the high proportion of serologically positive foals in winter is ascribed to the presence of large numb ... | 1993 | 7970572 |
| identification and characterization of the protein product of gene 71 in equine herpesvirus 1. | equine herpesvirus 1 (ehv-1) strain ab4 gene 71 is predicted to encode a primary product with a m(r) of 80.1k. we have previously constructed a deletion/lacz insertion mutant, ed71, and demonstrated that gene 71 is dispensable for growth of virus in cell culture. we have now constructed a gene 71 revertant, re71. to identify and characterize the product of gene 71, we produced a specific antiserum, anti-71, against a beta-galactosidase fusion protein containing the carboxy terminus of the gene 7 ... | 1994 | 7964621 |
| the gene downstream of the gc homologue in feline herpes virus type 1 is involved in the expression of virulence. | feline herpesvirus type 1 (fhv-1) mutants were constructed, carrying a beta-galactosidase marker gene integrated into the region downstream of the gene encoding the homologue of glycoprotein c (gc) of herpes simplex virus type 1. in cell culture, no differences in replication were observed between mutants and the parent fhv-1 strain. however, in experimentally infected cats, mutants caused fewer clinical signs after oronasal administration although they replicated to the same extent as the paren ... | 1994 | 7964620 |
| mapping, cloning and sequencing of a glycoprotein-encoding gene from bovine herpesvirus type 1 homologous to the ge gene from hsv-1. | in order to map and identify the glycoprotein-encoding gene from bovine herpesvirus type 1 (bhv-1), homologous to the ge glycoprotein from herpes simplex virus type 1 (hsv-1), a region of the unique short sequence from the bhv-1 genome has been sequenced. the sequenced region contains an orf coding for a polypeptide of 575 amino acids (aa). the aa sequence presents substantial similarity to that of the glycoprotein ge from hsv-1 and to homologous proteins of related viruses such as pseudorabies ... | 1994 | 7958994 |
| update on llama medicine. ophthalmology. | review of the limited literature on camelid eyes suggests they are anatomically similar to those of domestic livestock species, except they lack meibomian glands and have iridial folds (rather than corpora nigra). the microbial flora of the healthy camelid conjunctival sac also appears to be similar to those of domestic livestock and pets, except that no mycoplasma have been isolated from camelids. ocular diseases for which camelids are presented to veterinarians are numerous and varied. the mos ... | 1994 | 7953968 |
| expression and characterisation of equine herpesvirus 1 glycoprotein h using a recombinant baculovirus. | a recombinant baculovirus capable of expressing the glycoprotein h (gh) gene of equine herpesvirus 1 (ehv-1) was constructed. ehv-1 gh gene products in recombinant baculovirus infected insect cells were identified as 105 kda and 110 kda species compared with a 115 kda product detected in ehv-1 infected mammalian cells. the extent of n-glycosylation of ehv-1 gh in both insect and mammalian cells was indicated by a shift in apparent molecular weights after pngase f treatment to 90 kda and 95 kda f ... | 1994 | 7944958 |
| inhibition of feline immunodeficiency virus gene expression and replication by alphaherpesvirus icp4 homologues. | we investigated the effects of pseudorabies virus (prv), herpes simplex virus type 1 (hsv-1), and equine herpesvirus type 1 (ehv-1) icp4 homologues on feline immunodeficiency virus (fiv) long terminal repeat (ltr)-directed gene expression. this was done by using the transient expression chloramphenicol acetyltransferase (cat) assay in crandell feline kidney (crfk) and felis catus whole fetus 4 cells transfected with a chimeric fiv ltr-cat reporter construct in combination with effector plasmids ... | 1994 | 7931167 |
| comparison of the genomes of attenuated equine herpesvirus-1 strains with their parent virulent strain. | to elucidate the virulence factors of equine herpesvirus-1 (ehv-1), we compared the genomic architecture of the hh1 strain, a japanese isolate of ehv-1, with that of its four descendant strains obtained by serial passage in bovine kidney (bk) cells, i.e. bk77, bk161, bk271, and bk343. in preliminary studies high-passaged bk271 and bk343 lost virulence in colts, and in the present study digestion with restriction endonuclease bamhl showed that the dnas of bk271 and bk343 differed markedly from th ... | 1994 | 7909970 |
| clinical, serological and virological characteristics of an outbreak of paresis and neonatal foal disease due to equine herpesvirus-1 on a stud farm. | an outbreak of equine herpesvirus-1 (ehv-1) occurred on a large stud farm with 133 mares, 54 foals and four stallions, and at least 85 mares, 22 foals and three stallions were infected. clinical disease was observed in 16 mares, two stallions and 13 foals and the predominant clinical signs were scrotal oedema, ataxia and loss of libido in the stallions, ataxia and recumbency in the mares and uveitis and nasal discharge in the foals, although pneumonia and colic with intussusception were also rec ... | 1995 | 7900264 |
| identification and characterization of the protein product of gene 67 in equine herpesvirus type 1 strain ab4. | equine herpesvirus type 1 (ehv-1) strain ab4 gene 67 has no counterpart in any herpesvirus sequenced to date. to identify and characterize the product of ehv-1 gene 67, we have expressed the putative amino acids 11 to 260 encoded by gene 67 as a beta-galactosidase fusion protein in escherichia coli. the expressed fusion protein has been used to generate an antiserum raised against the gene 67 product. immunoblotting and immunoprecipitation experiments have revealed that the anti-67 serum specifi ... | 1995 | 7897346 |
| abortion due to equine herpesvirus in southern brazil. | we report an outbreak of abortion due to equine herpesvirus (ehv) in 5 mares between 9 and 11 months of gestation, from a herd of 22 thoroughbred mares. equine herpesvirus was isolated from extracts of the liver, spleen and thymus but not from the lungs of a 9-month fetus grown in rabbit kidney (rk13) cells. the virus was identified by electron microscopy, where virus particles could be seen in the nucleus of infected cells, and by the fluorescent antibody technique with polyclonal antibodies ag ... | 1994 | 7894345 |
| distribution of equid herpesvirus-1 (ehv-1) in respiratory tract associated lymphoid tissue: implications for cellular immunity. | twelve adult ponies and 2 conventional foals were exposed intranasally to ehv-1, strain ab4 (tcid50 10(-6.6) and samples of respiratory tract associated lymphoid tissues were recovered between 12 h and 13 days after infection. infectious virus was detected in tissue homogenates using susceptible cell monolayers and expression of viral antigens was monitored using indirect immunoperoxidase histochemistry on paraffin sections. the results showed both infectious ehv-1 and viral antigens in respirat ... | 1994 | 7889921 |
| distribution of equid herpesvirus-1 (ehv-1) in the respiratory tract of ponies: implications for vaccination strategies. | twelve adult ponies and 2 conventional foals were exposed to 10(6.6) tcid50 of equid herpesvirus-1 (ehv-1), strain ab4 and samples of respiratory tract tissues were recovered. infectious virus in tissue homogenates was detected using susceptible cell monolayers and expression of viral antigens was monitored using indirect immunoperoxidase histochemistry of paraffin sections. the results illustrated the rapid dissemination of ehv-1 throughout the respiratory tract, with early replication in the l ... | 1994 | 7889920 |
| another fence jumped in the ehv-1 stakes. | 1994 | 7889915 | |
| characterization and localization of the equine herpesvirus 1 major dna binding protein. | in previous studies of equine herpesvirus 1 (ehv-1) gene regulation, we observed an abundant early infected cell polypeptide (icp), designated icp130, which appeared in reduced amounts in cells infected with defective interfering particle-rich ehv-1 stocks compared to standard ehv-1-infected cells. to characterize this icp further, a monoclonal antibody (mab) was developed to ehv-1 icp130 and used to (1) affinity purify icp130, (2) examine icp130's ability to bind dna, and (3) define the synthes ... | 1995 | 7886942 |
| disseminated equine herpesvirus-1 infection in a two-year-old filly. | 1994 | 7858033 | |
| equine herpesviruses 1 and 4: amplification and differentiation by polymerase chain reaction. | 1994 | 7847200 | |
| [field trial with a subunit rhinopneumovaccine]. | 1995 | 7846673 | |
| an improved cosmid vector for the cloning of equine herpesvirus dna. | we have modified the commercial cosmid vector, triple helix vector (thv), such that i-sce-i restriction endonuclease sites flank the cloning site. i-sce-i is a rare-cutting endonuclease which recognizes an 18-bp sequence. it does not restrict the genome of either of the equine herpesvirus 1 or 4 (ehv-1 and ehv-4) strains we have cosmid cloned. thus, cosmid-cloned ehv fragments can be excised intact from the vector by i-sce-i digestion, facilitating production of large overlapping ehv fragments f ... | 1994 | 7821817 |
| amplification of strains of bovine herpesvirus 1 by use of polymerase chain reaction with primers in the thymidine kinase region. | a primer pair was designed from the published nucleotide sequence of the coding region of the bovine herpesvirus 1 (bhv-1) thymidine kinase (tk) gene for use in detection of the virus by use of polymerase chain reaction (pcr) amplification of 12 bhv-1 strains (3 atcc and 9 local isolates). a tk deletion mutant bhv-1, and 2 bhv-4 strains from atcc were used as negative controls. one strain each of feline herpes-virus, equine herpesvirus, and bovine adenovirus, and 2 noninoculated bovine cultured ... | 1994 | 7802385 |
| equine herpesvirus-1: a neurotropic alphaherpesvirus. | 1994 | 7801451 | |
| immunization with glycoprotein c of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model. | the glycoprotein c (gc) gene of equine herpesvirus-1 (ehv-1) was expressed in insect cells by a recombinant baculovirus as several products with apparent molecular weights of 66 kda-80 kda. the baculovirus ehv-1 gc products were recognised by monoclonal antibody and by ehv-1 convalescent equine sera, indicating conservation of antigenic determinants and confirming this glycoprotein as a target for the equine immune system. mice immunized with recombinant ehv-1 gc showed accelerated clearance of ... | 1995 | 7794119 |
| icp27 immediate early gene, glycoprotein k (gk) and dna helicase homologues of infectious laryngotracheitis virus (gallid herpesvirus 1) sa-2 strain. | a 4.8 kilobase segment located at the left-terminal in the unique long (ul) region of infectious laryngotracheitis virus (iltv) sa-2 strain contained three open reading frames (orfs). the first of 421 amino acids (aa) was located at map units 0.065 to 0.07, and its predicted 48 kilodaltons (kda) protein product has significant homology to the immediate early regulatory protein icp27 (ul54) of herpes simplex virus type-1 (hsv-1), to varicella-zoster virus (vzv) orf4 and to equine herpesvirus 1 (e ... | 1995 | 7794109 |
| equine herpesviruses 4 (equine rhinopneumonitis virus) and 1 (equine abortion virus). | 1995 | 7793324 | |
| equine herpesvirus 1 hvs25a isolated from an aborted foetus produces disease in balb/c mice. | 1995 | 7779039 | |
| serological relationship between a donkey alphaherpesvirus (isolate m7/91) and equid herpesvirus type 1 and 4. | rabbit hyperimmune serum prepared against a donkey alphaherpesvirus isolate (m7/91), and against ehv-1 and ehv-4 was used to characterise the antigenic relationship between these 3 viruses. serum from immunised rabbits was always more specific for homologous virus and showed different cross reactivity for heterologous virus. it was concluded that the immunologic relationship between the m7/91 isolate and ehv-1, was closer than that between this isolate and ehv-4. a serological survey of donkeys ... | 1994 | 7776336 |
| replication of equid herpesvirus 4 in endothelial cells and synovia of a field case of viral pneumonia and synovitis in a foal. | equid herpesvirus 4 (ehv-4) infection was diagnosed as the cause of interstitial pneumonia in a 6-week-old conventionally reared welsh pony foal, by cocultivation and immunolabelling with specific monoclonal antibodies, ehv-4 specific amplification of viral dna, and immunohistological examination of infected tissues. the case was novel in that replication of the ehv-4 isolate in endothelial cells and in the synovial epithelium was a feature. restriction digests of this isolate were compared with ... | 1995 | 7769144 |
| development of pcr assays to detect genetic variation amongst equine herpesvirus-1 isolates as an aid to epidemiological investigation. | a search for variable restriction sites has been carried out for equine herpesvirus-1 (ehv-1) in an attempt to develop markers which can be used to group epidemiologically related viruses into groups, and to learn more about the dynamics of ehv-1 disease. crude viral dna extracts of ehv-1, prepared by hirt extraction, were digested with alui, haeiii, or rsai, and southern blotted following electrophoresis. dna fingerprints, produced by probing the southern blots with the ehv-1 ecor1-i fragment, ... | 1995 | 7769032 |
| [neurological form of rhinopneumonitis in horses]. | 1995 | 7761973 | |
| expression of membrane-bound and secreted forms of equine herpesvirus 1 glycoprotein d by recombinant baculovirus. | analyses of the synthesis and processing of recombinant full-length glycoprotein d of equine herpesvirus type 1 (ehv-1; gd392) or recombinant truncated gd (gd352) expressed in baculovirus-infected sf9 cells revealed the following: (1) gd polypeptides encoded by both recombinant baculoviruses react with gd-specific antibodies including peptide-specific antiserum that neutralizes ehv-1 in a plaque reduction assay, (2) both the full-length recombinant gd392 and the truncated gd352 are expressed pre ... | 1995 | 7754672 |
| the dna sequence of human herpesvirus-6: structure, coding content, and genome evolution. | the complete dna sequence was determined for strain u1102 of human herpesvirus-6, a cd4+ t-lymphotropic virus with disease associations in immunodeficient settings and a possible complicating factor in aids. the genome is 159,321 bp in size, has a base composition of 43% g + c, and contains 119 open reading frames. the overall structure is 143 kb bounded by 8 kb of direct repeats, drl (left) and drr (right), containing 0.35 kb of terminal and junctional arrays of human telomere-like simple repea ... | 1995 | 7747482 |
| transcriptional analysis of the short segment of the feline herpesvirus type 1 genome and insertional mutagenesis of a unique reading frame. | transcription mapping was performed in the short region of the feline herpesvirus type 1 (fhv-1) genome as a first approach to the functional analysis of open reading frames encoding the homologs of the herpes simplex virus type 1 (hsv-1) gd, gl, ge, us9, and probably also us8.5. all reading frames appeared to be transcribed. transcripts were grouped into two nested rna sets; namely, the coterminal transcripts of gd and gl and the coterminal transcripts of ge, us8.5, and us9. this situation was ... | 1995 | 7747442 |
| protection against ehv-1 challenge infection in the murine model after vaccination with various formulations of recombinant glycoprotein gp14 (gb). | four formulations of the equine herpesvirus type 1 (ehv-1) glycoprotein gp 14 (gb), were tested for their ability to protect mice against intranasal (inas) ehv-1 challenge infection. the preparations tested included (i) a truncated gp14 produced in escherichia coli or (ii) a truncated gp14 expressed in insect cells by a recombinant baculovirus, (iii) truncated gp14 coexpressed with human immunodeficiency virus type 1 (hiv-1) gag virus-like particles (vlp) in insect cells, and (iv) a gp14-dna vac ... | 1995 | 7747423 |
| nuclear localization and transcriptional activation activities of truncated versions of the immediate-early gene product of equine herpesvirus 1. | the equine herpesvirus 1 (ehv-1) immediate-early (ie) gene product encodes a nuclear regulatory protein capable of negatively autoregulating its own promoter, transactivating representative ehv-1 early promoters, and acting in a concerted fashion with accessory ehv-1 regulatory factors to transactivate ehv-1 late promoters. to identify ie amino acid sequences involved in nuclear localization and to examine the contribution of c-terminal portions of the ie polypeptide to transactivation, vectors ... | 1995 | 7745735 |
| response of ponies to adjuvanted ehv-1 whole virus vaccine and challenge with virus of the homologous strain. | five yearling ponies were vaccinated with inactivated equid herpesvirus type 1 (ehv-1) in freund's complete adjuvant as a double emulsion and revaccinated 6 weeks later with ehv-1 in freund's incomplete adjuvant. these ponies and three age-matched controls were challenged intra-nasally after a further 6 weeks with homologous live virus and monitored clinically, biologically and serologically. after challenge, clinical signs were mild in both groups. no cell-associated viraemias were detected in ... | 1995 | 7735868 |
| a touchdown pcr for the differentiation of equine herpesvirus type 1 (ehv-1) field strains from the modified live vaccine strain rach. | more than 50 reference strains and field isolates of equine herpesvirus type 1 (ehv-1) were examined by a touchdown pcr. primers for specific amplification of ehv-1 dna were chosen from the terminal and internal repeat regions of the ehv-1 genome where the high-passaged live vaccine strain rach displays symmetric 850 bp deletions. the positive strand and one negative strand primer were designed to encompass the deletions present in rach, and the second negative strand primer was designed to hybr ... | 1994 | 7714035 |
| a type-specific serological test to distinguish antibodies to equine herpesviruses 4 and 1. | we describe a type-specific elisa, which distinguishes antibody to equine herpesvirus 4 (ehv4; equine rhinopneumonitis) and ehv1 (equine abortion virus) thereby identifying horses that have been infected with either or both of these antigenically related viruses. the antigens used are parts of the ehv4 and ehv1 glycoprotein g (gg) homologues expressed in e. coli as fusion proteins [crabb and studdert, 1993: j virol 67: 6332-6338). the expressed proteins comprise corresponding regions of the gg m ... | 1995 | 7710353 |
| detection and intracellular localization of equine herpesvirus 1 ir1 and ir2 gene products by using monoclonal antibodies. | during lytic infection, two transcripts arise from the equine herpesvirus 1 (ehv-1) immediate-early (ie) gene (ir1): a single, spliced 6.0-kb ie mrna and a 3'-coterminal 4.4-kb early mrna (ir2). previous studies demonstrated that transiently expressed ir1 and ir2 gene products are potent transcriptional regulators: ir1 proteins are capable of trans activating representative ehv-1 early and late promoters, while both ir1 proteins and the ir2 product, which lacks ir1 amino acid residues 1 to 322, ... | 1995 | 7707529 |
| regulatory function of the equine herpesvirus 1 icp27 gene product. | the ul3 protein of equine herpesvirus 1 (ehv-1) kya strain is a homolog of the icp27 alpha regulatory protein of herpes simplex virus type 1 (hsv-1) and the orf 4 protein of varicella-zoster virus. to characterize the regulatory function of the ul3 gene product, a ul3 gene expression vector (psvul3) and a vector expressing a truncated version of the ul3 gene (psvul3p) were generated. these effector plasmids, in combination with an ehv-1 immediate-early (ie) gene expression vector (psvie) and chi ... | 1995 | 7707500 |
| epitopes of glycoprotein g of equine herpesviruses 4 and 1 located near the c termini elicit type-specific antibody responses in the natural host. | specific serological diagnosis of equine herpesvirus 4 (ehv4; equine rhinopneumonitis virus) and ehv1 (equine abortion virus) hitherto has not been possible because of extensive antigenic cross-reactivity between these two closely related but distinct viruses. recently, we identified ehv4 glycoprotein g (gg) and characterized it as a type-specific, secreted glycoprotein (b. s. crabb, h. s. nagesha, and m. j. studdert, virology 190:143-154, 1992). this paper shows that ehv1 gg also possesses type ... | 1993 | 7690425 |
| a type-specific conformational epitope on the nucleocapsid of equid herpesvirus-1 and its use in diagnosis. | a type-specific monoclonal antibody was produced by immunizing mice with purified equid herpesvirus-1 (ehv-1). the ehv-1 specific mab reacted with all the ehv-1 strains tested so far by indirect elisa, immunofluorescence, and immunoperoxidase tests. no reactions were detected with the ehv-4, ehv-2, or ehv-3 strains tested. the indirect immunofluorescence and immunoperoxidase tests showed that the nuclei of infected cells were predominantly stained by this mab. triton treatment of the virus and i ... | 1993 | 7688948 |
| characterization of an antigenic site on glycoprotein 13 (gc) of equid herpesvirus type-1. | six monoclonal antibodies directed against ehv-1 glycoprotein 13 were characterized. five antibodies neutralized ehv-1 and were directed against a single antigenic site which comprised type-specific and type cross-reactive epitopes. inhibition of monoclonal antibody binding to this site by post-infection equine sera suggests that it is a target of host antibody during natural infection with either ehv-1 or ehv-4. | 1993 | 7682404 |
| zinc mining for protein domains. | 1994 | 7664042 | |
| cellular and antibody responses to equine herpesviruses 1 and 4. | 1995 | 7657564 | |
| equine herpesvirus 2 in pulmonary macrophages of horses. | in a search of viral agents in pulmonary macrophages of horses with chronic pulmonary disease, equine herpesvirus 2 was found to be unique. in 8 of 9 horses with chronic pulmonary disease, antigens of equine herpesvirus 2 were detected by indirect immunofluorescence staining of scattered foamy macrophages immediately after harvesting by bronchoalveolar lavage and fractionation on metrizamide gradients. in a healthy horse, antigens were not found. after 1 week of cultivation of bronchoalveolar la ... | 1995 | 7653883 |
| pseudorabies virus and equine herpesvirus 1 share a nonessential gene which is absent in other herpesviruses and located adjacent to a highly conserved gene cluster. | we have determined the nucleotide sequence and transcriptional pattern of a group of open reading frames in the pseudorabies virus (prv) genome located near the left end of the unique long region within bamhi 5' fragment at map positions 0.01 to 0.06. the 7,412-bp bamhi 5' fragment was found to contain five complete open reading frames and part of a sixth whose deduced amino acid sequences showed homology to the ul50 (partial), ul51, ul52, ul53, and ul54 gene products of herpes simplex virus typ ... | 1995 | 7637001 |
| herpes simplex virus immediate-early protein icp22 is required for viral modification of host rna polymerase ii and establishment of the normal viral transcription program. | infection of cells with herpes simplex virus type 1 (hsv-1) results in a rapid alteration of phosphorylation on the large subunit of cellular rna polymerase ii (rnap ii), most likely on its c-terminal domain (s. a. rice, m. c. long, v. lam, c. a. spencer, j. virol. 68:988-1001, 1994). this phosphorylation modification generates a novel form of the large subunit which we have designed iii. in this study, we examine roles that hsv-1 gene products play in this process. an hsv-1 mutant defective in ... | 1995 | 7637000 |
| characterization of the regulatory function of the icp22 protein of equine herpesvirus type 1. | the ir4 gene (inverted repeat gene 4) of equine herpesvirus type 1 (ehv-), the homolog of the herpes simplex virus type 1 icp22 gene, is differentially expressed as a 1.4-kb early transcript and a 1.7-kb late transcript that encode a series of proteins that migrate between 42 to 47 kda, localize to the nucleus of ehv-1-infected cells, and become packaged within ehv-1 virions (v. r. holden, g. b. caughman, y. zhao, r. n. harty, and d. j. o'callaghan, j. virol. 68, 4329-4340, 1994). to assess the ... | 1995 | 7618267 |
| sequence characteristics of a gene in infectious laryngotracheitis virus homologous to glycoprotein d of herpes simplex virus. | an infectious laryngotracheitis virus (iltv, gallid herpesvirus 1) gene homologous to glycoprotein d of herpes simplex virus (hsv) was identified and characterized by its nucleotide and derived amino acid sequence. the iltv gd gene is located in the unique short region (u(s)) and contains an open reading frame capable of specifying a polypeptide of 380 amino acids, including n- and c- terminal hydrophobic domains consistent with signal and anchor regions respectively, and no potential sites for ... | 1995 | 7612933 |
| transcriptional analyses of the unique short segment of ehv-1 strain kentucky a. | the unique short (us) segment of the genome of equine herpesvirus type 1 (ehv-1) strain kya is comprised of six open reading frames (orfs) that encode: a) a homolog of the us2 protein of herpes simplex virus type 1 (hsv-1); b) a serine threonine protein kinase that is a homolog of the hsv-1 us3 protein; c) a homolog of pseudorabies virus glycoprotein gx and hsv-2 gg; d) a novel glycoprotein, eus4, not encoded by other herpesviruses sequenced to date; e) a homolog of hsv-1 gd; and f) a homolog of ... | 1995 | 7597804 |
| guidelines for vaccination of horses. the american association of equine practitioners' vaccination guidelines subcommittee of the avma council on biologic and therapeutic agents. | 1995 | 7591939 | |
| application of an equine herpesvirus 1 (ehv1) type-specific elisa to the management of an outbreak of ehv1 abortion. | sera from 33 australian thoroughbred mares were tested during an outbreak of equine herpesvirus 1 (ehv1) abortion with an enzyme-linked immunosorbant assay (elisa) for the presence of ehv1-specific antibodies. the elisa used a recombinant ehv1 antigen derived from glycoprotein g (gg) and distinguished antibodies to ehv1 from those of the antigenically related and widespread herpesvirus ehv4. sera were obtained from most of the mares on three occasions, three, 13 and 67 days after the first abort ... | 1995 | 7571249 |
| the equine herpesvirus 1 gene 63 ring finger protein partially complements vmw110, its herpes simplex virus type 1 counterpart. | all alpha herpesviruses of known dna sequence have been found to encode a protein with similarities to immediate early protein vmw110 (icp0) of herpes simplex virus type 1 (hsv-1). the conserved portion of this family of proteins is a characteristic zinc binding module, known as a ring finger or c3hc4 domain. examples of ring finger domains occur in many other proteins of diverse evolutionary origin and function. recently, the solution structure of the equine herpesvirus 1 (ehv-1) ring finger pr ... | 1995 | 7561779 |
| a mouse model for testing the pathogenicity of equine herpes virus-1 strains. | a mouse model was developed for testing the pathogenicity of equine herpes virus-1 (ehv-1) strains. the model was validated with ehv-1 strains that are known to be of a low or high pathogenicity in horses. from all parameters tested, the safety index, which was calculated from the body weights of the mice after infection, proved to be the best predictive parameter. when this parameter was used, good and reliable correlations were found with the pathogenicity of the ehv-1 strains in horses. this ... | 1995 | 7559856 |
| cellular and antibody responses to equine herpesviruses 1 and 4 following vaccination of horses with modified-live and inactivated viruses. | the ability of monovalent and bivalent equine herpesvirus (ehv) vaccines to stimulate cellular and antibody responses to ehv-1 and ehv-4 was compared in healthy horses. comparison of data from lymphocyte blastogenesis tests in which live viruses were used as antigens and that were conducted prior to vaccination and after 2 vaccinations revealed that horses given modified-live ehv-1 had significant increases in proliferative responses to ehv-1 (p = 0.03) and ehv-4 (p = 0.04). responses to ehv-1 a ... | 1995 | 7538990 |
| use of lambda gt11 to identify antigenic components of equine herpesvirus 4. | a library of the equine herpesvirus 4 (ehv-4) genome was constructed in the lambda gt11 expression vector. recombinant bacteriophage expressing ehv-4 antigens as beta-galactosidase fusion proteins were detected with rabbit antiserum raised against ehv-4 virions and convalescent horse serum. ehv-4 dna sequences contained in the immunopositive recombinants were used as hybridization probes for mapping the genes encoding the antigens on the viral genome. the dna sequence of the probes was determine ... | 1994 | 7521096 |
| the nucleotide sequence of asinine herpesvirus 3 glycoprotein g indicates that the donkey virus is closely related to equine herpesvirus 1. | the nucleotide sequence of the glycoprotein g (gg) homologue of asinine herpesvirus 3 (ahv3), a respiratory alphaherpesvirus of donkeys, was determined. the ahv3 gg gene consists of 1233 base pairs (bp) and codes for a predicted protein of 411 amino acids. this is identical in size to the equine herpesvirus 1 (ehv1) gg gene and 6 amino acids longer than the equine herpesvirus 4 (ehv4) gg gene. the predicted amino acid sequence of ahv3 gg has characteristics of a class 1 membrane protein. the ami ... | 1995 | 7487497 |
| gp13 (ehv-gc): a complement receptor induced by equine herpesviruses. | equine herpesviruses type 1 (ehv-1) and type 4 (ehv-4) induce a complement receptor protein on the surface of infected cells capable of binding to the third component of complement (c3). the protein mediating the binding to the c3 component of complement was identified as glycoprotein 13 (gp13, ehv-gc), as expression of the cloned viral gene under the control of a cmv promoter induced c3 binding activity at the transfected cell surface. comparable to glycoprotein c (gc) from herpes simplex virus ... | 1995 | 7483825 |
| characterization of dna binding properties of the immediate-early gene product of equine herpesvirus type 1. | the equine herpesvirus type 1 (ehv-1) immediate-early (ie) gene encodes a phosphoprotein that is essential for the activation of transcription from viral early and late promoters and that regulates the transcription from its own promoter. employment of ehv-1 ie promoter dna probes and glutathione s-transferase fusion proteins harboring truncated portions of the ie gene product in gel shift assays, super shift assays with anti-ie monoclonal antibodies, and dnase i footprinting analyses revealed: ... | 1995 | 7483278 |
| identification and transcriptional analysis of a 3'-coterminal gene cluster containing ul1, ul2, ul3, and ul3.5 open reading frames of bovine herpesvirus-1. | we have identified and sequenced 3113 nucleotides located at the right end of the hindiii l fragment of the bovine herpesvirus-1 genome from map units 0.712 to 0.734. analysis of the sequence identified four open reading frames (orfs) which are designated ul1, ul2, ul3, and ul3.5 based on their homology with proteins of herpes simplex virus-1 (hsv-1), pseudorabies virus (prv), equine herpesvirus-1, and varicella-zoster virus. the ul1 orf of 158 amino acids exhibits limited homology with ul1 (gly ... | 1995 | 7483276 |
| equine herpesvirus 1 gene 12, the functional homologue of herpes simplex virus vp16, transactivates via octamer sequences in the equine herpesvirus ie gene promoter. | the hsv-1 transactivator of immediate-early (ie) gene expression, vp16, has several functional homologues among the alphaherpesviruses which have not yet been extensively studied in relation to their modes of action. to date, nothing is known of the exact sites or mechanism of interaction of the equine herpesvirus type 1 (ehv-1) homologue, the gene 12 protein, with the ehv-1 ie promoter. we show that the gene 12 protein utilises the promoter proximal region of the ie gene to induce activation an ... | 1995 | 7483272 |
| the varicella-zoster virus (vzv) open reading frame 47 (orf47) protein kinase is dispensable for viral replication and is not required for phosphorylation of orf63 protein, the vzv homolog of herpes simplex virus icp22. | to investigate the role of varicella-zoster virus (vzv) open reading frame 47 (orf47) protein kinase during infection, a vzv mutant was generated in which two contiguous stop codons were introduced into orf47, thus eliminating expression of the orf47 kinase. orf47 kinase was not essential for the growth of vzv in cultured cells, and the growth rate of the vzv mutant lacking orf47 protein was indistinguishable from that of parental vzv. nuclear extracts from cells infected with parental vzv conta ... | 1995 | 7474171 |
| role of the virion host shutoff (vhs) of herpes simplex virus type 1 in latency and pathogenesis. | the herpes simplex virus type 1 (hsv-1) ul41 gene product, virion host shutoff (vhs), has homologs among five alphaherpesviruses (hsv-1, hsv-2, pseudorabies virus, varicella-zoster virus, and equine herpesvirus 1), suggesting a role for this protein in neurotropism. a mutant virus, termed ul41nhb, which carries a nonsense linker in the ul41 open reading frame at amino acid position 238 was generated. ul41nhb and a marker-rescued virus, ul41nhb-r, were characterized in vitro and tested for their ... | 1995 | 7474089 |
| humoral and cell-mediated immune response of foals vaccinated with attenuated equine herpesvirus type 1 (ehv-1). | 1980 | 7223181 | |
| observations on vascular accidents in the central nervous system of neonatal foals. | a technique for the subarachnoid perfusion-fixation of the central nervous system was developed to help identify various significant vascular accidents (svas) in the central nervous system (cns) of 24 neonatal foals submitted for necropsy. svas, comprising subarachnoid, parenchymal and nerve root haemorrhages, and oedema and necrosis, occurred in 17 foals, more frequently in the spinal cord than the brain. they occurred as frequently in premature foals as in those born at full term, in foals bor ... | 1982 | 6962896 |
| ulcerative duodenitis in foals. | seven foals aged 18 days to 3 1/2 months had either single or multiple full-circumference segments or long antimesenteric bands of necrotizing duodenitis, sharply delineated from adjacent viable duodenum. perforation of the necrotic wall had occurred in all foals, leading to acute fibrinous peritonitis. on the mucosal surface severe diffuse, acute inflammation and ulceration involved the anterior half of the duodenum. two further foals, aged 28 and 30 days, had lesions that are believed to be a ... | 1983 | 6649336 |