Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| some additional data to the occurrence, morphology and validity of myxobolus turpisrotundus zhang, 2009 (myxozoa: myxosporea). | myxobolus turpisrotundus zhang, 2009, infects allogynogenetic gibel carp carassius auratus gibelio (bloch) and is always regarded as synonymous with myxobolus rotundus nemeczek, 1911, since its first report in goldfish carassius auratus auratus (l.) in china in 1955. in this study, it was comprehensively examined by morphological and molecular biological methods. the round spores of m. turpisrotundus are similar to those of m. rotundus from common bream abramis brama (l.) in morphology; however, ... | 2010 | 20352450 |
| chromium-induced biochemical, genotoxic and histopathologic effects in liver and kidney of goldfish, carassius auratus. | fish constitute an excellent model to understand the mechanistic aspects of metal toxicity vis-à-vis oxidative stress in aquatic ecosystems. hexavalent chromium (cr (vi)), due to its redox potential can induce oxidative stress (os) in fish and impair their health. in the present investigation, we hypothesize that os plays a key role in chromium induced toxicity in goldfish; leading to the production of reactive oxygen species (ros) such as o(.)(2), h(2)o(2), oh(.), and subsequent modulation of t ... | 2010 | 20348018 |
| effects of monocrotophos on the reproductive axis in the female goldfish (carassius auratus). | monocrotophos (mcp) is a highly toxic organophosphorus pesticide. to elucidate the influence of mcp on female fish reproduction, plasma 17beta-estradiol, testosterone and gonadotropin levels and aromatase and gonadotropin beta subunit gene expression levels were examined in female goldfish (carassius auratus) following a 21-day exposure to 0.01, 0.10 and 1.00 mg l-(1) 40% mcp-based pesticide in a semi-static exposure system. the results indicated that mcp induced increases in plasma 17beta-estra ... | 2010 | 20298809 |
| influence of temperature on the asphyxiation of young goldfish (carassius auratus l.) under various tensions of oxygen and carbon dioxide. | 1947 | 20249526 | |
| [epidemiological survey on clonorchiasis sinensis in an endemic area of south hunan province]. | to make an epidemiological investigation on clonorchiasis sinensis and relevant factors in the south of hunan province. | 2009 | 20232625 |
| evidence for a distributed respiratory rhythm generating network in the goldfish (carsssius auratus). | central pattern generators located in the brainstem regulate ventilatory behaviors in vertebrates. the development of the isolated brainstem preparation has allowed these neural networks to be characterized in a number of aquatic species. the aim of this study was to explore the architecture of the respiratory rhythm-generating site in the goldfish (carassius auratus) and to determine the utility of a newly developed isolated brainstem preparation, the sheep dip. here we provide evidence for a d ... | 2010 | 20217310 |
| inhibition of goldfish mitochondrial metabolism by in vitro exposure to cd, cu and ni. | although impairment of aerobic capacities has been reported in metal-contaminated wild fish, little is known about the direct toxicity of the metals themselves at the low concentrations found in the field compared to indirect consequences mediated by metal effects on ecological variables such as prey type and abundance, predation and competition. this study examined the in vitro effects of cd, cu and ni on mitochondrial enzyme activity and maximal (state 3) mitochondrial oxygen consumption rate ... | 2010 | 20207426 |
| e-screen and vitellogenin assay for the detection of the estrogenic activity of alkylphenols and trace elements. | the estrogenic potential of 4-nonylphenol (4-np), 4-octylphenol (4-op), p-t-octylphenol (p-t-op) and three trace elements, lead (pb), copper (cu) and cadmium (cd(no(3))(2) and cdcl(2)), were compared in two different tests, a proliferation assay with estrogen receptor-positive human mcf-7 breast cancer cells (e-screen) and the induction of vitellogenin (vtg) in juvenile goldfish (carassius auratus). the results showed differences in the bioassays' sensitivity and potency with the following order ... | 2010 | 20197112 |
| in vivo anthelmintic activity of crude extracts of radix angelicae pubescentis, fructus bruceae, caulis spatholobi, semen aesculi, and semen pharbitidis against dactylogyrus intermedius (monogenea) in goldfish (carassius auratus). | in search of a natural antiparasitic, in vivo anthelmintic activity of petroleum ether, chloroform, ethyl acetate, methanol, and aqueous extracts of angelica pubescens roots (radix angelicae pubescentis), brucea javanica fruits (fructus bruceae), spatholobus suberectus stems (caulis spatholobi), aesculus chinensis bge. seeds (semen aesculi), and pharbitis purpurea (l.) voigt seeds (semen pharbitidis) were tested against dactylogyrus intermedius (monogenea) in goldfish (carassius auratus). among ... | 2010 | 20191290 |
| active biomonitoring of complex pollution in taihu lake with carassius auratus. | carassius auratus bred in clean water were selected to conduct active biomonitoring (abm) exposures in meiliang and gong bays in the northern polluted area of taihu lake. the biotransformation enzymes 7-ethoxyresorufin-o-deethylase (erod) and glutathione s-transferase (gst), antioxidant enzymes glutathione peroxidase (gp(x)), reduced glutathione (gsh) content and superoxide dismutase (sod) and lipoperoxidation (as tbars) in liver were determined as biomarkers during the field exposure period. at ... | 2010 | 20189215 |
| pharmacokinetics of morphine after intramuscular injection in common goldfish carassius auratus and atlantic salmon salmo salar. | teleost fish have a nociceptive system and likely perceive pain. this warrants the development of analgesic protocols both for experimental surgery and for various husbandry procedures. morphine is the standard analgesic against which the efficacy of other analgesics is assessed, and is the analgesic that has been most used in fish. the aims of this study were to describe the pharmacokinetics of morphine after an intramuscular (i.m.) injection in common goldfish and atlantic salmon, and to illus ... | 2009 | 20183965 |
| effect of chemotherapy, vaccines and immunostimulants on innate immunity of goldfish infected with aeromonas hydrophila. | we report the effect of various chemicals, vaccines and immunostimulants on innate immune mechanisms such as phagocytosis activity, superoxide anion production of blood leukocytes, complement activity and plasma lysozyme activity and disease resistance in goldfish carassius auratus (23 +/- 2 g) against aeromonas hydrophila on days 15 and 30 post-infection. in infected fish, the administration of diets supplemented with either probiotics, tri-herbal extract or azadirachtin for 30 d significantly ... | 2009 | 20183964 |
| evolution of gustatory reflex systems in the brainstems of fishes. | the great number of species of teleosts permits highly specialized forms to evolve to occupy particular niches. this diversity allows for extreme variations in brain structure according to particular sensory or motor adaptations. in the case of the taste system, goldfish (carassius auratus l., 1758) and some carps have evolved a specialized intraoral food-sorting apparatus along with corresponding specializations of gustatory centers in the brainstem. a comparison of circuitry within the complex ... | 2009 | 20160963 |
| phytotherapy of ulcerative dermatitis induced by aeromonas hydrophila infection in goldfish ( carassius auratus ). | goldfish ( carassius auratus ) weighing 13 +/- 2 g were administered intramuscularly a sublethal dose (1.8 x 10 3 cfu/ml) of aeromonas hydrophila to induce ulcerative dermatitis. on day 3 and day 6 after infection the fish were dip-treated (for 5 min/day) with a tri-herbal concoction of curcuma longa, ocimum sanctum and azadirachta indica (1%). the ld 50 value was recorded at a concentration of 2.3 x 10 4 between 30 and 36 days after infection. in the infected untreated group the cumulative mort ... | 2010 | 20159736 |
| effects of monocrotophos on the reproductive axis in the male goldfish (carassius auratus): potential mechanisms underlying vitellogenin induction. | monocrotophos (mcp) is a highly toxic organophosphorus pesticide that has been banned in many countries. both vitellogenin mrna expression and secretion were significantly induced in male goldfish by exposure to an mcp-based pesticide, suggesting that mcp has significant estrogenic properties. to elucidate the mechanisms of action of mcp on vitellogenin induction, we used radioimmunoassay to examine the effect of mcp treatment on plasma 17beta-estradiol and testosterone levels in male goldfish ( ... | 2010 | 20149464 |
| the zalpha domain of pkz from carassius auratus can bind to d(gc)(n) in negative supercoils. | pkz was the most recently discovered member of eif2alpha kinase family in fish. capkz, the first identified fish pkz, possessed a conserved eif2alpha kinase catalytic domain in c-terminal and two z-dna binding domains (zalpha) in n-terminal. the zalpha of capkz closely resembled that of other z-dna binding proteins: adar1, dlm-1, and e3l. in order to understand more about the function of capkz, we expressed and purified three constructed peptides of capkz (p(zalpha)): p(zalpha1zalpha2), p(zalpha ... | 2010 | 20139004 |
| toxic effects of crude-oil-contaminated soil in aquatic environment on carassius auratus and their hepatic antioxidant defense system. | under the indoor simulant conditions, toxic effects of crude-oil-contaminated soil which was put into aquatic environment on the young fishes carassius auratus and their hepatic antioxidant system after a 20-d exposure were investigated. results showed that the relationship between the mortality of c. auratus and the exposed doses could be divided into 3 phases: fishes exposed to the low dose groups (0.5-5.0 g/l) were dead due to the ingestion of crude-oil-contaminated soils in aquatic environme ... | 2009 | 20108662 |
| susceptibility of koi x crucian carp and koi x goldfish hybrids to koi herpesvirus (khv) and the development of khv disease (khvd). | hybrids of koi, cyprinus carpio x crucian carp, carassius carassius and koi x goldfish, carassius auratus, proved to be susceptible to koi herpesvirus (khv, syn. cyhv-3) and developed khv disease (khvd). while hybrids of koi x goldfish were partly resistant to mortality following infection by immersion, most koi x crucian carp hybrids died after bath infection. khv dna was detected in dead fish but also in all surviving animals by different polymerase chain reactions (pcrs). according to these r ... | 2010 | 20102462 |
| biogeography and evolution of the carassius auratus-complex in east asia. | carassius auratus is a primary freshwater fish with bisexual diploid and unisexual gynogenetic triploid lineages. it is distributed widely in eurasia and is especially common in east asia. although several genetic studies have been conducted on c. auratus, they have not provided clear phylogenetic and evolutionary descriptions of this fish, probably due to selection bias in sampling sites and the dna regions analysed. as the first step in clarifying the evolutionary entity of the world's carassi ... | 2010 | 20064277 |
| characterization of non-cytosolic hexokinase activity in white skeletal muscle from goldfish (carassius auratus l.) and the effect of cold acclimation. | hk (hexokinase) is an enzyme involved in the first step in the glucose metabolism pathway, converting glucose into g6p (glucose 6-phosphate). owing to the importance of skeletal muscle for fish swimming and acclimation processes, we used goldfish (carassius auratus l.) white muscle in order to investigate subcellular distribution and kinetics of hk. in this study, we report that hk activity is predominantly localized in the mitochondrial fraction [nc-hk (non-cytosolic hk)] in goldfish white musc ... | 2010 | 20055755 |
| evaluation of dairy-yeast prebiotic supplementation in the diet of juvenile goldfish in the presence or absence of phytoplankton and zooplankton. | prebiotics recently have been shown to increase immune responses and disease resistance in certain fish species; therefore, the current study was conducted to evaluate the commercially available dairy-yeast prebiotic, grobiotic-a, for use with juvenile goldfish carassius auratus. the study consisted of two 10-week feeding trials in which juvenile goldfish were fed practical diets that were either unsupplemented or supplemented with the dairy-yeast prebiotic at 2% by dry weight. juvenile fish wer ... | 2009 | 20043400 |
| olfactory sensitivity to bile fluid and bile salts in the european eel (anguilla anguilla), goldfish (carassius auratus) and mozambique tilapia (oreochromis mossambicus) suggests a 'broad range' sensitivity not confined to those produced by conspecifics alone. | teleosts have high olfactory sensitivity to bile salts. to assess whether this phenomenon is involved in intra-specific chemical communication alone, or is part of a more ;broad range' sensitivity to bile salts produced by heterospecifics, we investigated possible differences in the odour of bile between the sexes and among different species - the eel (anguilla anguilla), goldfish (carassius auratus) and mozambique tilapia (oreochromis mossambicus) - using the electro-olfactogram (eog). we also ... | 2010 | 20038666 |
| isolation, cloning, sequencing of brain type aromatase and its expression in male and female wrasse, pseudolabrus sieboldi. | pseudolabrus sieboldi, wrasse being a diurnal spawner provides a good opportunity to study the endocrine mechanism of estrogen formation in brain and gonads. moreover, an extremely large amount of e2 was produced in serum and testis of wrasse. it is assumed that the presence of e2 may play a major role in diurnal gametogenesis in male fish. in this study brain type aromatase have been isolated, cloned and sequenced from the brain of wrasse. further, the expression pattern of brain type aromatase ... | 2005 | 20035447 |
| immune response and expression analysis of cathepsin k in goldfish during aeromonas hydrophila infection. | the innate immunity and expression profiles of cathepsins d were determined in the goldfish (carassius auratus) tissues after challenge with a fish pathogen aeromonas hydrophila. the innate immunity of reactive oxygen species (ros) and reactive nitrogen species (rns) were determined by peripheral blood leucocytes. blood and tissue samples of the muscle, gills, liver, kidney, heart, spleen, and intestine were sampled at 1, 3, 6 and 12 h post-infection for cathepsin d expression by semi-quantitati ... | 2010 | 20025977 |
| genetic, biochemical, and individual responses of the teleost fish carassius auratus to uranium. | carassius auratus were exposed for 96 h to different concentrations of uranyl nitrate (corresponding to 0, 100, 450, and 2,025 microg u l(-1)) and killed after different postexposure periods (0, 48, and 96 h) to assess uranium bioaccumulation, peroxisome proliferation (catalase [cat]), lipid peroxidation (thiobarbituric acid reactive substances [tbars]), and dna integrity in erythrocytes (comet assay). in addition, feeding behaviour was recorded as a general response to toxicant exposure. result ... | 2010 | 20012274 |
| prevention of ichthyophthirius multifiliis infestation in goldfish (carassius auratus) by potassium ferrate(vi) treatment. | ichthyophthirius multifiliis is an important freshwater teleost pathogen that often leads to significant economic losses to the aquaculture industry. the purpose of this study was to assess the acute toxicity of potassium ferrate(vi) to i. multifiliis theront and the concentration needed to prevent i. multifiliis infestation in goldfish, carassius auratus. five hundred theronts were exposed to concentrations of potassium ferrate(vi) in each well of a 96-well microtiter plate and observed for 4h ... | 2010 | 20006444 |
| physiological dimensions of ototoxic responses in a model fish species. | pharmaceutical agents known to be toxic to the human auditory system also impair sensory hair cells of teleosts, and this supports the use of fish models for the screening of such compounds. however, previous investigations have focused almost exclusively on anatomical changes after drug administration without assessing macro-level physiological effects. using the goldfish (carassius auratus), we demonstrate that the acquisition of auditory evoked potentials offers a rapid and non-invasive means ... | 2010 | 20004583 |
| oxidative stress and apoptosis of carassius auratus lymphocytes induced by nonplanar (pcb153) and coplanar (pcb169) polychlorinated biphenyl congeners in vitro. | among all the 209 kinds of polychlorinated biphenyls (pcbs) congeners, nonplanar and coplanar pcb congeners have different levels of toxicity on mammal cells such as neuronal cells, but little is known about their toxicity on fish cells although pcb congeners usually have high bioaccumulation abilities in the detected fish bodies. this study showed that 2,2',4,4',5,5'-hexacholorbiphenyl (pcb153, nonplanar congener) and 3,3',4,4',5,5'-hexacholorbiphenyl (pcb 169, coplanar congener) caused apoptos ... | 2009 | 19999978 |
| methyltestosterone-induced changes in electro-olfactogram responses and courtship behaviors of cyprinids. | in the tinfoil barb (barbonymus schwanenfeldii; family cyprinidae), we previously found that increased olfactory sensitivity to a female prostaglandin pheromone could induce sexual behavior display in juvenile fish treated with androgens. here, we determined if this phenomenon is widespread among cyprinid fishes by adding 17alpha-methyltestosterone (mt) to aquaria containing juveniles of 4 cyprinid species (tinfoil barbs; redtail sharkminnows, epalzeorhynchos bicolor; goldfish, carassius auratus ... | 2010 | 19965901 |
| herbal supplementation diets on hematology and innate immunity in goldfish against aeromonas hydrophila. | goldfish, carassius auratus (47 + or - 3 g, n = 300) were inoculated intramuscularly (50 microl) with aeromonas hydrophila (1.8 x 10(6) cells ml(-1)). on the 6th day of post-infection the fishes were divided into i) control, without infection fed with normal diet (c), ii) infected fish, fed with normal diet (iu), and infected fishes treated with different doses of iii) 100 mg kg(-1), iv) 200 mg kg(-1), iv) 400 mg kg(-1) and vi) 800 mg kg(-1) mixed herbal extracts supplementation diets. hematolog ... | 2010 | 19941960 |
| serotonergic synapses modulate generation of spikes from retinal ganglion cells of teleosts. | serotonin [5-hydroxytryptamine (5-ht)] is a common neurotransmitter/neuromodulator found widely in the nervous system. cellular morphology and retinal distribution of serotonergic amacrine cells in the channel catfish (ictalurus punctatus) retina are identified using monoclonal anti-5ht antibody. these cells receive ribbon synapses from off-center (hyperpolarizing) bipolar cells as well as conventional synapses with other non-serotonergic amacrine cells. output synapses from the serotonergic cel ... | 2009 | 19938208 |
| effect of acute copper sulfate exposure on olfactory responses to amino acids and pheromones in goldfish (carassius auratus). | exposure of olfactory epithelium to environmentally relevant concentrations of copper disrupts olfaction in fish. to examine the dynamics of recovery at both functional and morphological levels after acute copper exposure, unilateral exposure of goldfish olfactory epithelia to 100 microm cuso(4) (10 min) was followed by electro-olfactogram (eog) recording and scanning electron microscopy. sensitivity to amino acids (l-arginine and l-serine), generally considered food-related odorants, recovered ... | 2009 | 19924975 |
| distribution of central melatonin binding sites in the goldfish (carassius auratus). | in fish, as well as in birds and mammals, the pineal hormone melatonin acts as a neuroendocrine transducer of photoperiod and as such has a profound influence over the timing of seasonal behavior, especially reproduction. with the aim of better understanding how melatonin may exert its influence in the fish, we have studied the distribution and characteristics of the melatonin binding sites in the brain and pituitary of the goldfish (carassius auratus) using the ligand [2-(125)i]iodomelatonin an ... | 1991 | 19912786 |
| utilization of tissue habitats by myxobolus wulii landsberg & lom, 1991 in different carp hosts and disease resistance in allogynogenetic gibel carp: redescription of m. wulii from china and japan. | myxobolus wulii (=myxosoma magna) was first described from the gills of goldfish, carassius auratus auratus, in china. subsequently, a myxosporean infecting the hepatopancreas of allogynogenetic gibel carp, c. auratus gibelio, was designated as a different species, myxobolus guanqiaoensis, although the morphological features were almost identical to those of m. wulii. in japan, an unidentified myxobolus sp. was found in the gills and hepatopancreas of goldfish. morphological and molecular analys ... | 2010 | 19912459 |
| cytosolic distribution of cd, cu and zn, and metallothionein levels in relation to physiological changes in gibel carp (carassius auratus gibelio) from metal-impacted habitats. | in the present study the impact of environmental metal contamination in gibel carp (carassius auratus gibelio) was investigated coupling disturbances in subcellular metal distribution to effect biomarkers. gibel carp were caught at six different sampling sites in flanders (belgium), characterized by different degrees in environmental metal contamination. tissue cd, cu and zn concentrations and total metallothioneon (mt) levels were determined in gills, liver and kidney. cytosolic metal distribut ... | 2010 | 19900706 |
| are all koi ulcer cases associated with infection by atypical aeromonas salmonicida? polymerase chain reaction assays of koi carp skin swabs submitted by hobbyists. | infection by atypical aeromonas salmonicida is regarded as the cause of ulcer disease (kud) in koi carp cyprinus carpio and goldfish carassius auratus. however, other causes--including parasites, viral infection, and fungi--have been proposed. in our diagnostic work, we often fail to isolate a. salmonicida even when clear clinical signs of kud are present. this failure may be because these fastidious and slow-growing bacteria are difficult to isolate in culture or because the bacteria are not ac ... | 2009 | 19873831 |
| bioassay-guided isolation and identification of active compounds from fructus arctii against dactylogyrus intermedius (monogenea) in goldfish (carassius auratus). | dactylogyrus intermedius is a significant monogenean parasite on the gills of cyprinid fishes and can cause serious problem in fish aquaculture. in the present study, bioassay-guided fractionation was employed to identify the active compounds from fructus arctii against d. intermedius. five solvents (petroleum ether, chloroform, ethyl acetate, ethanol, and water) were applied for the extraction of fructus arctii. among them, only the chloroform extract exhibited promising anthelmintic efficacy a ... | 2009 | 19859737 |
| [experimental establishment of life cycle of clonorchis sinensis]. | to establish and maintain the life cycle of clonorchis sinensis in laboratory. | 2009 | 19856506 |
| effect of oral immunization with aeromonas hydrophila ghosts on protection against experimental fish infection. | to investigate whether oral immunization with aeromonas hydrophila ghosts (ahg) vaccine can elicit mucosal and systemic immune responses of carp (carassius auratus gibelio) compared to conventional formalin-killed bacteria (fkc). | 2010 | 19843213 |
| the goldfish sg2na gene encodes two alpha-type regulatory subunits for pp-2a and displays distinct developmental expression pattern. | sg2na is a member of the striatin protein family. in human and mouse, the sg2na gene encodes two major protein isoforms: sg2na alpha and sg2na beta. the functions of these proteins, except for acting as the regulatory subunits for pp-2a, remain largely unknown. to explore the possible functions of sg2na in lower vertebrates, we have isolated two sg2na cdnas from goldfish, carassius auratus. our results reveal that the first cdna contains an orf of 2118 bp encoding a deduced protein with 705 amin ... | 2009 | 19838339 |
| characterization of a novel tc1-like transposon from bream (cyprinidae, megalobrama) and its genetic variation in the polyploidy progeny of bream-red crucian carp crosses. | crossing the cyprinids diploid blunt snout bream megalobrama amblycephala (bsb) and carassius auratus red var. (rcc) generated sterile triploid (3nrb) and fertile tetraploid (4nrb) hybrid offspring. utilizing inverted terminal repeats (itrs) of transposon tdr1 from danio rerio as pcr primer, the results showed that evident change in the number of tc1-like transposons in 4nrb relative to bsb occurred, whereas such change did not arise in 3nrb compared to bsb. no tc1-like transposon was found in r ... | 2009 | 19826746 |
| calcium oscillations in the olfactory nonsensory cells of the goldfish, carassius auratus. | the olfactory nonsensory cells contribute to the maintenance of normal functions of the olfactory epithelium (oe). specifically, the ciliated nonsensory cells of teleosts play important roles in the odorant detection by oe in aqueous environment. their cilia show strong beating activities and cause water flow at the oe surface, making the detection of odorants by oe more efficient. because intracellular ca2+ level has been reported to play an important role in ciliary beating, the ciliary beatin ... | 2009 | 19800938 |
| cell growth-promoting activity of fluid from eye sacs of the bubble-eye goldfish (carassius auratus). | the growth-promoting effects of fish body fluids, such as serum and embryonic extract, on fish cell cultures have been widely demonstrated. the bubble-eye variety of aquarium goldfish is characterized as having a large sac filled with fluid (sac fluid) under each eye. these sacs are believed to contain lymph, which is similar in composition to serum or blood plasma. in order to test whether the sac fluid can be used as an additive for fetal bovine serum (fbs) in growth factor supplements, we com ... | 2009 | 19798918 |
| modulatory effects of deltamethrin on antioxidant defense mechanisms and lipid peroxidation in carassius auratus gibelio liver and intestine. | pyrethroids, such as deltamethrin, are toxic substances that lead to generation of reactive oxygen species, which harm living organisms. we assessed the level and patterns of imbalance evolved by a single dose of 2 microg/l deltamethrin on the lipid peroxidation (lpo) and the antioxidant defense system of carassius auratus gibelio liver and intestine, and monitored the recovery dynamics of these parameters during a 14-day post-exposure period. lpo and antioxidative defense mechanisms displayed d ... | 2010 | 19789906 |
| development of larval contracaecum rudolphii hartwich, 1964 (ascaridida: anisakidae) in experimentally infected goldfish (carassius auratus l., 1758). | laboratory-bred goldfish were experimentally infected with contracaecum rudolphii, either directly (with nematode larvae) or indirectly via infected zooplankton. intensity and prevalence of infection were markedly higher in fish exposed to the infected zooplankton than in goldfish exposed to nematode larvae. when transmitted to fish via the zooplankton, larvae developed much faster and, after 8 weeks, became firmly encysted in the intestine wall. the digestive tract of larvae isolated from the i ... | 2010 | 19781129 |
| trichlorfon-induced apoptosis in hepatocyte primary cultures of carassius auratus gibelio. | trichlorfon, an organophosphorus pesticide, can disrupt metabolism, reproduction and immune functions of some aquatic animals. in the present study, the effect of trichlorfon on apoptosis and the underlying apoptotic mechanism were investigated in primary cultures of carassius auratus gibelio hepatocytes. analyses of cultures exposed to 0, 0.01, 0.1, and 1.0 mg l(-1) trichlorfon concentrations for 24h indicated that trichlorfon induced apoptosis and caused nuclear shrinkage, cell membrane ruptur ... | 2009 | 19775726 |
| influence of cu2+-loaded silicate on the growth performance and microflora of crucian carp carassius auratus. | we investigated the effects of cu2+-loaded silicate (cls) on the growth performance, microflora of skin, gill and intestine, and intestinal morphology of crucian carp carassius auratus. a total of 225 native wild crucian carp, with an average initial body weight of 20 g, were randomly divided into 5 treatment groups using 3 replicate tanks of 15 fish per group. the dietary treatments were (1) basal diet, (2) basal diet + cuso4, (3) basal diet + silicate, (4) basal diet + 0.5% cls and (5) basal d ... | 2009 | 19750812 |
| molecular cloning and differential expression patterns of the regulatory subunit b' gene of pp2a in goldfish, carassius auratus. | it is well established that the protein serine/threonine phosphatase 2a (pp2a) plays very important roles in many different cellular processes, including cell proliferation and differentiation, gene expression, neurotransmission, apoptosis, and aging. pp2a consists of three heterogenic subunits: the scaffold subunit a, the catalytic subunit c, and the regulatory subunit b. while both the scaffold and the catalytic subunits contain only two forms, at least four families of the regulatory subunits ... | 2009 | 19727590 |
| question of reference frames: visual direction-selective neurons in the accessory optic system of goldfish. | we investigated if visual direction-selective neurons in the pretectal area (apt) of goldfish (carassius auratus auratus) preferred visual stimuli resulting from rotations around axes corresponding to the best responsive axes of the semicircular canals [optic flow that is consistent to a maximal activation of the horizontal canal pair (yaw), to a maximal activation of the right anterior/left posterior semicircular canal pair (ralp), and to a maximal activation of the left anterior/right posterio ... | 2009 | 19710380 |
| epr detection of hydroxyl radical generation and its interaction with antioxidant system in carassius auratus exposed to pentachlorophenol. | in the present study, direct evidence of hydroxyl radical production in livers of carassius auratus exposed to pentachlorophenol (pcp) was provided using electron paramagnetic resonance (epr) with spin-trapping. a dose-effect relationship was obtained between hydroxyl radical intensities and pcp exposure. it was observed that hydroxyl radical was significantly induced by 0.001 mg l(-1) (below the criteria for chinese fishery water quality) of pcp exposure. a strong positive correlation (r=0.9581 ... | 2009 | 19683864 |
| vsx1 3' untranslated region-mediated translation difference at different developmental stages of goldfish embryos. | visual system homeobox-1 (vsx1) is important in retinal progenitor proliferation, differentiation, and function maintenance of bipolar cells in vertebrates. recent study in xenopus laevis has shown that vsx1 3' untranslated region (3' utr) can mediate cell-specific translation of vsx1 mrna in the bipolar cells of the retinal inner nuclear layer (inl). vsx1 is also transcribed at the early developmental stages prior to eye formation and its spatiotemporal expression patterns are conserved in all ... | 2009 | 19683671 |
| intraperitoneal injection of extracted microcystins results in hypovolemia and hypotension in crucian carp (carassius auratus). | circulatory responses of crucian carp injected intraperitoneally with extracted microcystins (mcs) were studied at sublethal and lethal doses (150 and 600 microg mc kg(-1) body mass, respectively). mean arterial blood pressure (map), heart rate, hematocrit (hct), red blood cell (rbc) counts, and circulating blood volume (bv) were assayed at 0, 1, 3, 12, 24, and 48 h post-toxin administration. map decreased significantly in a dose-dependent manner over time. within the 48-h test period, the letha ... | 2009 | 19673078 |
| identification of estrogen-like effects and biologically active compounds in river water using bioassays and chemical analysis. | the nackdong river is the longest river in south korea and passes through major cities that have several industrial complexes, including chemical, electric, and petrochemical complexes, and municipal characteristics such as apartment complexes. along the river, the gumi region has an electric industrial complex and an apartment complex that may be possible point sources of xenoestrogens such as phenolic compounds. to identify the causative chemicals for estrogenic activity in the river water of ... | 2009 | 19647290 |
| morphological and molecular phylogenetic analysis of two saprolegnia sp. (oomycetes) isolated from silver crucian carp and zebra fish. | two saprolegnia isolates, jy isolated from silver crucian carp (carassius auratus gibelio bloch) and bmy isolated from zebra fish (brachydanio rerio hamilton) came from infections occurring concurrently in different locations in china. to confirm whether the two isolates were from the same saprolegnia clone, comparative studies have been carried out based on their morphological, physiological and molecular characteristics. observations showed that morphologically (both asexual and sexual organs) ... | 2009 | 19640399 |
| molecular characterization and functional commonality of nucleophosmin/nucleoplasmin in two cyprinid fish. | nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. to clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cdna sequence from two cyprinid fish, carassius auratus gibelio and carassius auratus. molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. rt-pcr and western blot detected a specific expression in gonads, and immunofluorescence localization r ... | 2009 | 19634010 |
| effects of ecf-kraft pulp mill effluent treated with fungi (rhizopus oryzae) on reproductive steroids and liver cyp1a of exposed goldfish (carassius auratus). | the toxicity of bleached kraft pulp mill effluents (bkme) is usually attributed to chemical compounds which are produced and released throughout various stages of pulp and paper production. the main objective of the present work was to detect sub-lethal responses of goldfish (carassius auratus) to secondary treated bkme which was treated with rhizopus oryzae. a total of 96 carps (c. auratus; 11 +/- 3 g) were exposed to different concentrations of the post-treated effluent (0, 1, 10, 25, 50, and ... | 2009 | 19621256 |
| innate immune response and disease resistance in carassius auratus by triherbal solvent extracts. | this study reports the effect of aqueous, ethanol and methanol triherbal solvent extract from azadirachta indica, ocimum sanctum and curcuma longa on innate immune mechanisms such as phagocytosis activity, respiratory burst activity, alternative complement activity and lysozyme activity and disease resistance in goldfish (carassius auratus) against aeromonas hydrophila. fish were intraperitoneally injected with different doses of 0, 5, 50 and 100 mg kg(-1) body weight of each triherbal solvent e ... | 2009 | 19616631 |
| does speaker presentation affect auditory evoked potential thresholds in goldfish? | the auditory evoked potential (aep) recording technique has proved to be a very versatile and successful approach in studying auditory sensitivities in fishes. the aep protocol introduced by kenyon, ladich and yan in 1998 using an air speaker with the fish positioned at the water surface gave auditory thresholds in goldfish very close to behavioural values published before. this approach was subsequently modified by several laboratories, raising the question whether speaker choice (air vs. under ... | 2009 | 19602445 |
| molecular characterization of novel interferon gamma receptor 1 isoforms in zebrafish (danio rerio) and goldfish (carassius auratus l.). | interferon gamma (ifngamma) is a highly pleotropic pro-inflammatory and anti-viral cytokine that mediates its effects by binding to a receptor complex composed of interferon gamma receptors 1 and 2 (ifngr1 and ifngr2). using gene synteny analysis, we identified a distinct isoform of the zebrafish ifngr1. the two zebrafish ifngr1 called here ifngr1-1 and ifngr1-2 were used to identify the respective cdna sequences of the goldfish ifngr1-1 and ifngr1-2. analysis of protein sequences revealed that ... | 2009 | 19577303 |
| expression regulation and functional characterization of a novel interferon inducible gene gig2 and its promoter. | grass carp hemorrhagic virus (gchv)-induced gene 2 (gig2) is a novel gene previously identified from uv-inactivated gchv-treated carassius auratus blastulae embryonic (cab) cells, suggesting that it should play a pivotal role in the interferon (ifn) antiviral response. in this study, a polyclonal anti-gig2 antiserum was generated and used to study the inductive expression pattern by western blot analysis, showing no basal expression in normal cab cells but a significant up-regulation upon uv-ina ... | 2009 | 19573926 |
| the behavioural, digestive and metabolic characteristics of fishes with different foraging strategies. | to test the hypothesis that digestion has a more notable physiological effect on ambush foragers than on active foragers, we investigated the behavioural, digestive and metabolic characteristics, as well as the postprandial locomotory capacity, of four species of juvenile fish distributed along the yangtze river, china, with distinct foraging strategies. the ambush foraging southern catfish (silurus meridionlis) had the fewest movements per minute (mpm), lowest per cent time spent moving (ptm), ... | 2009 | 19561220 |
| genomic organization and expression analysis of toll-like receptor 3 in grass carp (ctenopharyngodon idella). | toll-like receptor 3 (tlr3) participates in the innate immune response by recognizing viral pathogens. to investigate grass carp immune system responding to gcrv (grass carp reovirus) infection, the full-length cdna sequence and genomic organization of grass carp tlr3 (citlr3) was identified and characterized. the full-length genome sequence of citlr3 is composed of 5668 nucleotides, including five exons and four introns. the full-length of citlr3 cdna is 3681 bp in length and encodes a polypept ... | 2009 | 19545638 |
| melatonin attenuates the acetylcholine-induced contraction in isolated intestine of a teleost fish. | the present study investigates the possible direct actions of melatonin (n-acetyl-5-methoxytryptamine) on intestinal motility in goldfish (carassius auratus) using an in vitro system of isolated intestine in an organ bath engaged to an isometric transducer. the longitudinal strips from goldfish intestine in the organ bath showed a resting spontaneous myogenic rhythmic activity which is not altered by melatonin. the addition of acetylcholine (1 nmol l(-1)-10 mmol l(-1)) to the organ bath induces ... | 2009 | 19543897 |
| molecular and functional characterization of kita and kitla of the goldfish (carassius auratus l.). | kit ligand and its type iii tyrosine kinase receptor kit promotes the survival, proliferation and differentiation of progenitor cells involved in mammalian myelopoiesis. in this study we report on the molecular and functional characterization of kit receptor a (kita) and kit ligand a (kitla) from the goldfish. both kita and kitla were ubiquitously expressed in goldfish tissues, with higher mrna levels observed in the kidney and spleen, the major hematopoietic organs of fish. furthermore, both ki ... | 2009 | 19527751 |
| trypanosoma carassii hsp70 increases expression of inflammatory cytokines and chemokines in macrophages of the goldfish (carassius auratus l.). | we report on the cloning and characterization of trypanosoma carassii 70 kda heat shock protein (hsp70). t. carassii hsp70 was secreted/excreted into culture medium in vitro and was recognized by sera from infected fish. recombinant hsp70 (rhsp70) activated goldfish macrophages and stimulated the production of pro-inflammatory cytokines including interferon gamma (ifngamma), tumor necrosis factor (tnf)-alpha, interleukin (il)-1beta, (il)-12 and chemokines ccl-1 and cxcl-8 (il-8). t. carassii hsp ... | 2009 | 19527750 |
| evolutionary conservation of dazl genomic organization and its continuous and dynamic distribution throughout germline development in gynogenetic gibel carp. | to investigate germline development and germ cell specification, we identified a dazl homolog (cagdazl) from gynogenetic gibel carp (carassius auratus gibelio). its cdna sequence and bac clone sequence analyses revealed the genomic organization conservation and conserved synteny of the dazl family members and their neighborhood genes among vertebrates, especially in fish. moreover, a polyclonal antibody specific to cagdazl was produced and used to examine its expression and distribution througho ... | 2009 | 19504540 |
| the role of telencephalic no and cgmp in avoidance conditioning in goldfish (carassius auratus). | our previous study with n-methyl-d-aspartate (nmda) receptor antagonist d-ap5 suggested that nmda receptors were involved in learning but not memory consolidation of avoidance conditioning. the present study investigated whether nitric oxide (no) and cyclic guanosine monophosphate (cgmp) were involved in memory consolidation but not learning of avoidance conditioning in goldfish. experiments 1 to 3 investigated amnestic and performance effects of no inhibitor l-name and cgmp inhibitor ly-83583. ... | 2009 | 19485568 |
| development of a polymerase chain reaction assay to detect cyprinid herpesvirus 2 in goldfish. | cyprinid herpesvirus 2 (cyhv2) has been associated with epidemic mortality among cultured populations of goldfish carassius auratus. as the principal target tissues are hematopoietic cells in the kidney and spleen, the disease is designated herpesviral hematopoietic necrosis (hvhn). originally described from japan, the virus is present in at least five other countries and probably has a global distribution in goldfish. preventing the further spread of the virus via control programs that exploit ... | 2009 | 19485127 |
| trematode centrocestus formosanus infection and distribution in ornamental fishes in mexico. | the aim of this study was to determine the ornamental fish species affected by the metacercariae of the digenean trematode centrocestus formosanus and its distribution in 48 fish farms in morelos, central mexico. the parasite was found to form various numbers of branchial cysts in 11 of the 25 species analyzed. goldfish carassius auratus was the most commonly affected species; 20 of 30 farms were positive for this parasite, the fish showing severe clinical signs and having a high mortality rate. ... | 2009 | 19485122 |
| glucose-6-phosphate isomerase is an endogenous inhibitor to myofibril-bound serine proteinase of crucian carp (carassius auratus). | glucose-6-phosphate isomerase (gpi) was purified to homogeneity from the skeletal muscle of crucian carp ( carassius auratus ) by ammonium sulfate fractionation, column chromatographies of q-sepharose, sp-sepharose, and superdex 200 with a yield of 8.0%, and purification folds of 468. the molecular mass of gpi was 120 kda as estimated by gel filtration, while on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), two subunits (55 and 65 kda) were identified, suggesting that it ... | 2009 | 19472999 |
| [effects of nonylphenol on proliferation and anti-oxidative functions of carassius auratus primary culture hepatecytes]. | the study on the proliferation and anti-oxidative functions of carassius auratus primary culture hepatecytes under effects of different concentration nonylphenol showed that at all test concentrations of nonylphenol, the proliferation of the hepatecytes was inhibited. high concentration (10(-3) mol x l(-1)) nonylphenol had significant inhibitory effect, and induced an apparent morphological alteration of the hepatecytes. nonylphenol upset the balance of hepatecytes anti-oxidative system through ... | 2009 | 19459375 |
| physiological consequences of gill remodeling in goldfish (carassius auratus) during exposure to long-term hypoxia. | goldfish (carassius auratus) acclimated to 7 degrees c and exposed to hypoxia ( approximately 10 mmhg) for 7 days exhibited a pronounced remodeling of the gill consisting of the removal of an interlamellar cell mass (ilcm). subsequent experiments were designed to assess the impact of gill remodeling and the associated increase in functional lamellar surface area on the distribution of branchial ionocytes and cl(-) flux across the gill. despite the increased functional lamellar surface area durin ... | 2009 | 19458280 |
| nitric oxide-cgmp signaling regulates axonal elongation during optic nerve regeneration in the goldfish in vitro and in vivo. | nitric oxide (no) signaling results in both neurotoxic and neuroprotective effects in cns and pns neurons, respectively, after nerve lesioning. we investigated the role of no signaling on optic nerve regeneration in the goldfish (carassius auratus). nadph diaphorase staining revealed that nitric oxide synthase (nos) activity was up-regulated primarily in the retinal ganglion cells (rgcs) 5-40 days after axotomy. levels of neuronal nos (nnos) mrna and protein also increased in the rgcs alone duri ... | 2009 | 19457064 |
| interplay of the endocannabinoid system with neuropeptide y and corticotropin-releasing factor in the goldfish forebrain. | based on pharmacological, behavioral, and neuroanatomical studies, the endocannabinoid system appears to be pivotal in some neuroendocrine mechanisms, such as modulation of vertebrate reproduction, stress, and food intake. the present study aimed to investigate the involvement of the endocannabinoid system in the control of the feeding response in the goldfish. by means of immunohistochemistry techniques, using anti-cb1 cannabinoid receptor, anti-corticotropin-releasing factor (crf), and anti-ne ... | 2009 | 19456363 |
| low toxic herbicide roundup induces mild oxidative stress in goldfish tissues. | the formulation of roundup consists of the herbicide glyphosate as the active ingredient with polyethoxylene amine added as a surfactant. the acute toxicity of roundup (particularly of glyphosate) to animals is considered to be low according to the world health organization, but the extensive use of roundup may still cause environmental problems with negative impact on wildlife, particularly in an aquatic environment where chemicals may persist for a long time. therefore, we studied the effects ... | 2009 | 19450865 |
| estrogenic effects of monocrotophos evaluated by vitellogenin mrna and protein induction in male goldfish (carassius auratus). | monocrotophos (mcp) is listed as a prior informed consent chemical, and it is still used extensively in developing countries. it has been seen that mcp shows high toxicity in fish and aquatic ecosystems. to confirm whether mcp shows estrogenic effects on fish, the induction of vitellogenin (vtg) in male goldfish (carassius auratus) following a 21 day exposure to 0.01, 0.10 and 1.00 mg x l(-1) 40% mcp pesticide in a semi-static exposure system was evaluated by hepatic mrna and plasma protein. rea ... | 2009 | 19439194 |
| molecular cloning and functional characterization of the first non-mammalian 26rfa/qrfp orthologue in goldfish, carassius auratus. | in this study, we used data mining approach to predict 26rfa/qrfp precursors from fish, amphibian, reptile and avian species and subsequently cloned a 26rfa/qrfp precursor cdna from goldfish brain based on the predicted sequences information. the goldfish 26rfa/qrfp precursor cdna encoded a propeptide of 168 amino acids (aa) with predicted signal peptide of 30 aa at n-terminal and putative mature peptides, including 26rfa (26 aa) and 7rfa (7 aa) located at the c-terminal. multiple sequence align ... | 2009 | 19428995 |
| in vitro generation of viral-antigen dependent cytotoxic t-cells from ginbuna crucian carp, carassius auratus langsdorfii. | little is known about antigen-specific t-cell responses to viruses in teleosts due to a lack of a suitable experimental system using inbred or clonal animals. in the present study we have successfully induced an in vitro generation of virus-specific cytotoxic t-cells (ctls) from isogeneic ginbuna crucian carp. responder cells (primarily lymphocytes) from crucian carp haematopoietic necrosis virus (chnv)-infected fish were capable of proliferating after stimulation in vitro with chnv-infected syn ... | 2009 | 19428040 |
| apelin in goldfish (carassius auratus): cloning, distribution and role in appetite regulation. | apelin is a recently discovered peptide produced by several tissues including brain and adipose tissue. in mammals and zebrafish, apelin regulates cardiovascular functions. recent evidence in mammals suggest that apelin might also regulate food intake. in this study, we cloned a cdna encoding apelin and examined apelin mrna distribution within the brain and in peripheral tissues. we also assessed the effects of fasting on apelin brain mrna abundance. apelin mrna was expressed throughout the brai ... | 2009 | 19427346 |
| evolutionary history of the somatostatin and somatostatin receptors. | somatostatin and its receptors have a critical role in mammalian growth through their control pattern of secretion of growth hormone, but the evolutionary history of somatostatin and somatostatin receptors are ill defined. we used comparative whole genome analysis of danio rerio, carassius auratus, xenopus tropicalis, gallus gallus, monodelphis domestica, homo sapiens, sus scrofa, bos taurus, mus musculus, rattus norvegicus, canis lupus familiaris, ovis aries, equus caballus, pan troglodytes and ... | 2009 | 19417543 |
| embryonic and developmental toxicity of the ionic liquid 1-methyl-3-octylimidazolium bromide on goldfish. | the embryonic developmental toxicity of the ionic liquid (il) 1-methyl-3-octylimidazolium bromide ([c(8)mim]br) on the goldfish carassius auratus was evaluated in this study. first, the 72 h 50% lethal concentrations (72 h-lc(50)) for [c(8)mim]br in goldfish embryos at the stages of cleavage, early gastrula, closure of blastopore, and heart beating were determined by preliminary acute toxicity tests. after that, fish embryos in different developmental stages (cleavage, early gastrula, closure of ... | 2010 | 19415752 |
| chlorpheniramine impairs spatial choice learning in telencephalon-ablated fish. | this work investigated the effect of the h1 receptor blockade in the forebrain of ablated carassius auratus in a simple stimulus-response learning task using a t-maze test with positive reinforcement. the goldfish were submitted to surgery for removal of both telencephalic lobes five days before beginning the experiment. a t-shaped glass aquarium was employed, with two feeders located at the extremities of the long arm. one of the two feeders was blocked. the experimental trials were performed i ... | 2008 | 19399346 |
| molecular cloning and expression of two melanin-concentrating hormone receptors in goldfish. | melanin-concentrating hormone (mch) is a neurohypophysial hormone and induces melanin aggregation in the skin in teleosts. mch also has multiple roles in the central regulation of food intake in teleosts and mammals. mch receptors (mch-r) are among type i g-protein-coupled receptors. here, we cloned two mch receptors from goldfish, carassius auratus. the amino acid sequence of goldfish mch-r1 had 57-88% homology with fish mch-r1 and 49-50% homology with mammalian mch-r1, while the amino acid seq ... | 2009 | 19397943 |
| the life cycle of myxobolus lentisuturalis (myxozoa: myxobolidae), from goldfish (carassius auratus auratus), involves a raabeia-type actinospore. | we studied a natural infection of the oligochaete branchiura sowerbyi beddard, 1892 with the raabeia-type actinosporean stage of myxobolus lentisuturalis dyková, fiala et nie, 2002 which infected goldfish carassius auratus auratus (l.) in italy, using molecular analysis of the ssu rrna gene. the existence of intraoligochaete development shows that this parasite follows the life-cycle pattern described by wolf and markiw (1984) for myxobolus cerebralis. histological examinations of the goldfish i ... | 2009 | 19391327 |
| co-culture of carp (cyprinus carpio) kidney haematopoietic cells with feeder cells resulting in long-term proliferation of t-cell lineages. | to characterise fish haematopoietic stem/progenitor cells, it is necessary to develop a culture system that supports proliferation and differentiation of these cells. in the present study, we established cell lines from various tissues of carp (cyprinus carpio) and ginbuna (carassius auratus langsdorfii). by using these cell lines, we developed a culture system in which carp haematopoietic cells proliferated and were successively passaged. cell lines from carp thymus (kot), carp fin (kof1) and g ... | 2009 | 19375173 |
| the detection of pressure fluctuations, sonic audition, is the dominant mode of dipole-source detection in goldfish (carassius auratus). | behavioral detection of a low-frequency (40 hz) vibratory dipole at source distances of 1.5-24 cm was measured by classically conditioned respiratory suppression in goldfish (carassius auratus). detection thresholds were compared across distances and before and after ablation of individual octavolateralis sensory channels. detection thresholds, expressed in units of pressure (spl), remained roughly constant as distance between the stimulus source and animal increased. lateral line inactivation, ... | 2009 | 19364230 |
| pulp and paper mill effluents contain neuroactive substances that potentially disrupt neuroendocrine control of fish reproduction. | here we show for the first time that components of pulp and paper mill effluents contain neuroactive substances that may impair fish reproduction. grab samples of primary and secondary effluent were obtained from a representative pulp and paper mill in eastern canada. effluents were fractionated using classic polarity and polyphenolic extraction methods into solvents of selected polarities (water, ethanol, ethyl acetate, and hexane). by means of in vitro, competitive assays on goldfish (carassiu ... | 2009 | 19350947 |
| circadian clock genes of goldfish, carassius auratus: cdna cloning and rhythmic expression of period and cryptochrome transcripts in retina, liver, and gut. | clock genes are known to be the molecular core of biological clocks of vertebrates. they are expressed not only in those tissues considered central pacemakers, but also in peripheral tissues. in the present study, partial cdnas for 6 of the principal clock genes (period 1-3 and cryptochrome 1-3) were cloned from a teleost fish, the goldfish (carassius auratus ). these genes showed high homology (approximately 90%) with the respective cdnas of zebrafish (danio rerio), the only other teleost from ... | 2009 | 19346448 |
| experimental infection of carassius auratus (l., 1758) with the second stage larvae of the nematode contracaecum rudolphii hartwich, 1964. | susceptibility of the goldfish to infection with the newly hatched second stage larvae of contracaecum rudolphii was investigated under laboratory condition. a week after the larvae had been placed in the fish tank, the autopsied goldfish showed the nematodes in their intestine lumen. the majority of the larvae were dead. in week 2 and 3 of exposure, the larvae were being found exclusively in the intestinal wall; in week 4 and 5, the larvae, in addition to being present in the intestinal wall, w ... | 2008 | 19338227 |
| chlorpheniramine impairs functional recovery in carassius auratus after telencephalic ablation. | we determined the effect of an h1 receptor antagonist on the functional recovery of carassius auratus submitted to telencephalic ablation. five days after surgery the fish underwent a spatial-choice learning paradigm test. the fish, weighing 6-12 g, were divided into four groups: telencephalic ablation (a) or sham lesion (s) and saline (sal) or chlorpheniramine (cpa, ip, 16 mg/kg). for eight consecutive days each animal was trained individually in sessions separated by 24 h (alternate days). tra ... | 2009 | 19330266 |
| distribution of ghrelin peptide in the gastrointestinal tract of stomachless and stomach-containing teleosts. | the occurrence and localization of ghrelin peptide in the gastrointestinal tract of carassius auratus and dicentrarchus labrax, two fish species that exhibit different feeding behavior, different habitats, and different anatomical organizations of the gastroenteric tract, were examined by immunohistochemical methods and western blotting analysis. all of the gastrointestinal segments studied displayed immunohistochemical localizations of ghrelin peptide. numerous single or clustered immunoreactiv ... | 2009 | 19322896 |
| first description of a novel weissella species as an opportunistic pathogen for rainbow trout oncorhynchus mykiss (walbaum) in china. | six strains of gram-positive, catalase-negative, non-motile, irregular short rod-shaped weissella bacteria, with width and length of 0.5-0.6 and 1.2-2.7microm were isolated from diseased rainbow trout oncorhynchus mykiss (walbaum) in winter of 2007 at a commercial fishery in jingmen, hubei province, china. the diseased rainbow trout exhibited hemorrhage in eyes, anal region, intestine and abdomen wall, petechia of liver, some fish with hydrocele in stomach. six isolates had identical biochemical ... | 2009 | 19321272 |
| herpesviral haematopoietic necrosis virus (cyhv-2) infection: case studies from commercial goldfish farms. | herpesviral haematopoietic necrosis is a disease of goldfish, carassius auratus, caused by cyprinid herpesvirus-2 (cyhv-2) infection. quantitative pcr was carried out on tissue homogenates from healthy goldfish fingerlings, broodfish, eggs and fry directly sampled from commercial farms, from moribund fish submitted to our laboratory for disease diagnosis, and on naturally-infected cyhv-2 carriers subjected to experimental stress treatments. healthy fish from 14 of 18 farms were positive with cop ... | 2009 | 19309419 |
| structural and functional multiplicity of the kisspeptin/gpr54 system in goldfish (carassius auratus). | to ascertain the neuroendocrine function of the kisspeptin/gpr54 system in non-mammalian species, full-length cdnas encoding for kiss1 and kiss2 as well as their putative cognate receptors gpr54a and gpr54b, were isolated from goldfish (carassius auratus). the deduced protein sequences between kiss1 and kiss2 in goldfish share very low similarity, but their putative mature peptides (kisspeptin-10) are relatively conserved. rt-pcr analysis demonstrated that the goldfish kiss1 gene (gfkiss1) is hi ... | 2009 | 19304758 |
| etiology and pathology of epidermal papillomas in allogynogenetic crucian carp carassius auratus gibelio (female) x cyprinus carpio var. singuonensis (male). | allogynogenetic crucian carp carassius auratus gibelio (female) x cyprinus carpio var. singuonensis (male) is one of the main freshwater aquaculture species in china. in recent years, epidermal papillomas have been observed on the scales, fins and opercula of adult fish in many fish farms in the chongming county of shanghai, china. the disease appears in the late autumn of the first year and becomes more severe in winter. it gradually regresses in the late spring or summer of the second year, as ... | 2009 | 19301639 |
| goldfish cb1 mrna expression is affected by fasting and anandamide administration. | the endocannabinoid system has a well-documented pivotal role in the control of mammalian feeding response; nevertheless, some evidence is available regarding a similar role in nonmammalian vertebrates and invertebrates. as in the bonyfish carassius auratus, cb1 cannabinoid receptors are abundant in brain regions involved in the control of food intake, and fasting affects endocannabinoid levels, in this study the effects of food deprivation and anandamide administration on cb1 expression were ev ... | 2009 | 19293731 |
| [study on the endocrine disrupting effect of dimethachlon]. | in order to study the endocrine disrupting effect of dimethachlon (ndps) (estrogenic and antiandrogenic activity). | 2009 | 19267069 |
| predominant expression and cellular distribution of fish agr2 in renal collecting system. | anterior gradient 2 (agr2) genes encode secretory proteins, and play significant roles in anterior-posterior patterning and tumor metastasis. agr2 transcripts were shown to display quite diverse tissue distribution in different species, and little was known about the cellular localization of agr2 proteins. in this study, we identified an agr2 homologue from gibel carp (carassius auratus gibelio), and revealed the expression patterns and cellular localization during embryogenesis and in adult tis ... | 2009 | 19266620 |
| influence of dietary feeding of low monomer content grape seed extract on vitellogenin production and cholesterol levels in goldfish, carassius auratus. | grape seed extract (gse) is a source of naturally occurring compounds known as proanthocyanidins and flavan-3-ols, which are recognized to exert a protective effect on human health, so gse is widely used mainly as a nutritional supplement. however, polyphenols may have, in some cases, estrogenic effects or may interfere with the endocrine system. for that reason, it was considered of interest to investigate the beneficial or detrimental effects induced by low monomer content grape seed extract ( ... | 2009 | 19256555 |
| crystallization and preliminary x-ray crystallographic studies of the z-dna-binding domain of a pkr-like kinase (pkz) in complex with z-dna. | pkz, a pkr-like eif2alpha kinase, consists of a z-dna-specific binding domain (zalpha) and an eif2alpha kinase domain. the kinase activity of pkz is modulated by the binding of zalpha to z-dna. the mechanisms underlying z-dna binding and the subsequent stimulation of pkz raise intriguing questions. interestingly, the z-dna-binding domain of pkz from goldfish (carassius auratus; cazalpha(pkz)) shows limited sequence homology to other canonical zalpha domains, suggesting that it may have a distinc ... | 2009 | 19255480 |