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age-dependent transcriptional and epigenomic responses to light exposure in the honey bee brain.light is a powerful environmental stimulus of special importance in social honey bees that undergo a behavioral transition from in-hive to outdoor foraging duties. our previous work has shown that light exposure induces structural neuronal plasticity in the mushroom bodies (mbs), a brain center implicated in processing inputs from sensory modalities. here, we extended these analyses to the molecular level to unravel light-induced transcriptomic and epigenomic changes in the honey bee brain. we h ...201627398303
parasites modulate within-colony activity and accelerate the temporal polyethism schedule of a social insect, the honey bee.task allocation in social insect colonies is generally organised into an age-related division of labour, termed the temporal polyethism schedule, which may in part have evolved to reduce infection of the colony's brood by pests and pathogens. the temporal polyethism schedule is sensitive to colony perturbations that may lead to adaptive changes in task allocation, maintaining colony homeostasis. though social insects can be infected by a range of parasites, little is known of how these parasites ...201727397965
palaearctic hoplitis bees of the subgenera formicapis and tkalcua (megachilidae, osmiini): biology, taxonomy and key to species.formicapis and tkalcua are related subgenera of the osmiine bee genus hoplitis (megachilidae) containing four and three species, respectively. h. (formicapis) species inhabit open forests and windfalls mainly in the boreal zone with one species distributed across the holarctic and three species occurring in eastern asia. h. (tkalcua) species live in deserts and semideserts from the canary island and northern africa eastwards to the arabian peninsula. analysis of female pollen loads, field observ ...201627395614
family bombyliidae.bombyliidae is one of the largest diptera families with more than 4,500 recognized species worldwide. their species vary from robust to thin, and may be small to large (2-20mm) and looks like bees or wasps. they also present great variation in color. adults can often be seen either resting and sunning themselves on trails, rocks or twigs or feeding on flowering plants as they are nectar feeders. all reared bee flies are predators or parasitoids of arthropods. the colombian fauna of bombyliids co ...201627395279
a new genus and species of micro bee fly from the earliest eocene french amber (diptera: mythicomyiidae: psiloderoidinae).mythicomyiidae, or micro bee flies, are tiny flies (0.5-5.0 mm) that are found throughout most parts of the world except the highest altitudes and latitudes (greathead & evenhuis 2001). including all extinct and extant taxa, the mythicomyiidae currently comprise more than 380 valid taxonomic species distributed among 30 genera. the subfamily psiloderoidinae is especially well represented among the fossil mythicomyiidae by seven cretaceous or cenozoic genera. we here describe a new genus and a ne ...201627395149
taxonomic revision of the afrotropical bee genus serapista cockerell (hymenoptera: apoidea: megachilidae: megachilinae: anthidiini).the sub-saharan bee genus serapista cockerell is revised. it comprises six species. serapista pernigra cockerell is restored as a valid species, s. aspernigra eardley and griswold, new species, is described from the eastern part of southern africa and serapista rhodesiana mavromoustakis is synonymized with s. pernigra. descriptions, keys for identification, distributional records and floral associations of the species of serapista are given.201627395094
hoplitis (hoplitis) galichicae spec. nov., a new osmiine bee species from macedonia with key to the european representatives of the hoplitis adunca species group (megachilidae, osmiini).hoplitis (hoplitis) galichicae spec. nov., a new european osmiine bee species belonging to the hoplitis adunca species group (osmiini) is described and diagnosed. it is currently known only from the galichica mountain range in southwestern macedonia. analysis of pollen contained in the metasomal scopae revealed that all females of the type series collected pollen on the flowers of sedum (crassulaceae), which is unexpected as most other members of the hoplitis adunca species group are oligolectic ...201627394906
biology of paenibacillus larvae, a deadly pathogen of honey bee larvae.the gram-positive bacterium paenibacillus larvae is the etiological agent of american foulbrood of honey bees, a notifiable disease in many countries. hence, p. larvae can be considered as an entomopathogen of considerable relevance in veterinary medicine. p. larvae is a highly specialized pathogen with only one established host, the honey bee larva. no other natural environment supporting germination and proliferation of p. larvae is known. over the last decade, tremendous progress in the under ...201627394713
fumagillin control of nosema ceranae (microsporidia:nosematidae) infection in honey bee (hymenoptera:apidae) colonies in argentina.information on the long‑term consequences of nosema ceranae to honey bee lifespan and effectiveness of nosema control with fumagillin is scarce and not always consistent. our objective in this study was to evaluate the effectiveness of the antibiotic fumagillin to control n. ceranae in hives in east‑central argentina. honey bee hives were assigned to 3 experimental treatments, a control group with un‑treated hives, a preventive strategy group with hives treated monthly, and a monitoring strategy ...201627393876
preparation of water and ethanolic extracts of propolis and evaluation of the preparations.propolis was extracted using water and various concentrations of ethanol as solvents. the extracts were investigated by measurement of absorption spectrum with a uv spectrophotometer, reversed phase-high pressure thin-layer chromatography and reversed phase-hplc. maximum absorption of all extracts was 290 nm, resembling flavonoid compounds, and the 80% ethanolic extract showed highest absorption at 290 nm. the most isosakuranetin, quercetin, and kaempferol were extracted from mixtures of propoli ...199827393593
species-specific diagnostics of apis mellifera trypanosomatids: a nine-year survey (2007-2015) for trypanosomatids and microsporidians in serbian honey bees.in this study, honey bees collected in serbia over 9 consecutive years (2007-2015) were retrospectively surveyed to determine the prevalence of eukaryotic gut parasites by molecular screening of archival dna samples. we developed species-specific primers for pcr to detect the two known honey bee trypanosomatid species, crithidia mellificae and the recently described lotmaria passim. these primers were validated for target specificity under single and mixed-species conditions as well as against t ...201627392956
expression analysis of egr-1 ortholog in metamorphic brain of honeybee (apis mellifera l.): possible evolutionary conservation of roles of egr in eye development in vertebrates and insects.specific genes quickly transcribed after extracellular stimuli without de novo protein synthesis are known as immediate early genes (iegs) and are thought to contribute to learning and memory processes in the mature nervous system of vertebrates. a recent study revealed that the homolog of early growth response protein-1 (egr-1), which is one of the best-characterized vertebrate iegs, shared similar properties as a neural activity-dependent gene in the adult brain of insects. with regard to the ...201627392711
contrasting pollinators and pollination in native and non-native regions of highbush blueberry production.highbush blueberry yields are dependent on pollination by bees, and introduction of managed honey bees is the primary strategy used for pollination of this crop. complementary pollination services are also provided by wild bees, yet highbush blueberry is increasingly grown in regions outside its native range where wild bee communities may be less adapted to the crop and growers may still be testing appropriate honey bee stocking densities. to contrast crop pollination in native and non-native pr ...201627391969
bee venom phospholipase a2 ameliorates motor dysfunction and modulates microglia activation in parkinson's disease alpha-synuclein transgenic mice.α-synuclein (α-syn) has a critical role in microglia-mediated neuroinflammation, which leads to the development of parkinson's disease (pd). recent studies have shown that bee venom (bv) has beneficial effects on pd symptoms in human patients or 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (mptp) toxin-induced pd mice. this study investigated whether treatment with bv-derived phospholipase a2 (bvpla2) would improve the motor dysfunction and pathological features of pd in human a53t α-syn mutant ...201627388550
history of research on pharmacopuncture in korea.this study introduces the history and types of korean pharmacopuncture and reports trends of research on korean pharmacopuncture.201627386142
a nicotinic acetylcholine receptor agonist affects honey bee sucrose responsiveness and decreases waggle dancing. 201627385759
virion structure of israeli acute bee paralysis virus.the pollination services provided by the western honeybee (apis mellifera) are critical for agricultural production and the diversity of wild flowering plants. however, honeybees suffer from environmental pollution, habitat loss, and pathogens, including viruses that can cause fatal diseases. israeli acute bee paralysis virus (iapv), from the family dicistroviridae, has been shown to cause colony collapse disorder in the united states. here, we present the iapv virion structure determined to a r ...201627384649
proteome analysis of the hemolymph, mushroom body, and antenna provides novel insight into honeybee resistance against varroa infestation.varroa destructor has been identified as a major culprit responsible for the losses of millions of honeybee colonies. varroa sensitive hygiene (vsh) is a suite of behaviors from adult bees to suppress mite reproduction by uncapping and/or removing mite infested pupae from a sealed brood. despite the efforts to elucidate the molecular underpinnings of vsh, they remain largely unknown. we investigated the proteome of mushroom bodies (mbs) and antennae of adult bees with and without vsh from a stoc ...201627384112
macelignan inhibits bee pathogenic fungi ascophaera apis growth through hog1 pathway.ascosphaera apis is a bee pathogen that causes bee larvae infection disease, to which treatment is not yet well investigated. the aim of this study was to investigate antifungal susceptibility in vitro against a. apis and to identify a new antifungal agent for this pathogen through minimal inhibitory concentration (mic) assay and western blot analysis. macelignan had 1.56 and 3.125 μg/ml mic against a. apis after 24 and 48 h, respectively, exhibiting the strongest growth inhibition against a. ap ...201627383123
an experimental approach for optimizing coating parameters of electroless ni-p-cu coating using artificial bee colony algorithm.this paper aims to present an experimental investigation for optimum tribological behavior (wear depth and coefficient of friction) of electroless ni-p-cu coatings based on four process parameters using artificial bee colony algorithm. experiments are carried out by utilizing the combination of three coating process parameters, namely, nickel sulphate, sodium hypophosphite, and copper sulphate, and the fourth parameter is postdeposition heat treatment temperature. the design of experiment is bas ...201427382630
flower detection and acuity of the australian native stingless bee tetragonula carbonaria sm.we tested the endemic australian tetragonula carbonaria bee as a model of how colour vision may allow these small bees to find flowers. in a y-maze apparatus, we presented stimuli that contained both chromatic- and green-receptor contrasts, or only had chromatic contrast to free flying bees. stimuli were detected at visual angles of 9.5° and 9.3°, respectively. we next made morphological measurements of the compound eye under high magnification using a digital microscope, and despite a relativel ...201627380933
fatty acid profiles of monofloral clover beebread and pollen and proteomics of red clover (trifolium pratense) pollen.fatty acids were identified in monofloral beebread (bb) and bee pollen (bp) loads collected from trifolium pratense l. a gas chromatography method was used to identify and quantify fatty acids: thirty-five fatty acids were identified in bb and 42 in bp. a high amount of the healthy n-3 fatty acids was found. the ratio of polyunsaturated fatty acids n-3 to n-6 reached a value of 8.42 and 3.35 in the latter products. the proteomic analysis also was performed on the manually collected t. pratense p ...201627380113
secondary biomarkers of insecticide-induced stress of honey bee colonies and their relevance for overwintering strength.the evaluation of pesticide side-effects on honeybees is hampered by a lack of colony-level bioassays that not only are sensitive to physiological changes, but also allow predictions about the consequences of exposure for longer-term colony productivity and survival. here we measured 28 biometrical, biochemical and behavioural indicators in a field study with 63 colonies and 3 apiaries. colonies were stressed in early summer by feeding them for five days with either the carbamate growth regulato ...201627376353
odor experiences during preimaginal stages cause behavioral and neural plasticity in adult honeybees.in eusocial insects, experiences acquired during the development have long-term consequences on mature behavior. in the honeybee that suffers profound changes associated with metamorphosis, the effect of odor experiences at larval instars on the subsequent physiological and behavioral response is still unclear. to address the impact of preimaginal experiences on the adult honeybee, colonies containing larvae were fed scented food. the effect of the preimaginal experiences with the food odor was ...201627375445
predominant api m 10 sensitization as risk factor for treatment failure in honey bee venom immunotherapy.component resolution recently identified distinct sensitization profiles in honey bee venom (hbv) allergy, some of which were dominated by specific ige to api m 3 and/or api m 10, which have been reported to be underrepresented in therapeutic hbv preparations.201627372568
first detection of apis mellifera filamentous virus in apis cerana cerana in china.although many honey bee rna viruses have been correlated with colony declines, little is known regarding the potential role of dna viruses. here, we examined seemingly healthy and crawling bee samples from china using pcr to identify whether apis mellifera filamentous virus (amfv) was present in a. cerana cerana. the highest amfv infection percentage among chinese provinces occurred in crawling bees from gansu province (85.48%), and the lowest was in bees from beijing (31.58%). a phylogenetic an ...201627369386
pollination by sexual deception-it takes chemistry to work.semiochemicals are of paramount importance in sexually deceptive plants. these plants sexually lure specific male insects as pollinators by chemical and physical mimicry of the female of the pollinator. the strategy has evolved repeatedly in orchids, with a wide diversity of insect groups exploited. chemical communication systems confirmed by field bioassays include: alkenes and alkanes in bee pollinated ophrys species, keto-acid and hydroxy-acids in scoliid wasp pollinated o. speculum, and cycl ...201627368084
the consumption of propolis and royal jelly in preventing upper respiratory tract infections and as dietary supplementation in children.propolis and royal jelly (rj), two important honeybee products, have been used commonly all over the world as traditional and ethnopharmacological nutrients since ancient times. both of them have a lot of active ingredients which are known to be effective for several medical conditions. in this article, medical databases were searched for the usage of rj and propolis in upper respiratory tract infections (urti) and as a dietary supplementation, together and separately. 10-hydroxy-2-decenoic acid ...201727366357
multiple bee stings resulting in st elevation myocardial infarction (the kounis syndrome).kounis syndrome consists of angina pectoris or myocardial infarction that is triggered by the release of inflammatory mediators in the setting of an allergic reaction. we present the case of a 61-year-old man who presented to the emergency department with anaphylaxis after being stung by >100 bees. during resuscitation, he subsequently developed st elevation myocardial infarction.201627365877
multiple locus variable number of tandem repeat analysis: a molecular genotyping tool for paenibacillus larvae.american foulbrood, caused by paenibacillus larvae, is the most severe bacterial disease of honey bees (apis mellifera). to perform genotyping of p. larvae in an epidemiological context, there is a need of a fast and cheap method with a high resolution. here, we propose multiple locus variable number of tandem repeat analysis (mlva). mlva has been used for typing a collection of 209 p. larvae strains from which 23 different mlva types could be identified. moreover, the developed methodology not ...201627365124
conservation in mammals of genes associated with aggression-related behavioral phenotypes in honey bees.the emerging field of sociogenomics explores the relations between social behavior and genome structure and function. an important question is the extent to which associations between social behavior and gene expression are conserved among the metazoa. prior experimental work in an invertebrate model of social behavior, the honey bee, revealed distinct brain gene expression patterns in african and european honey bees, and within european honey bees with different behavioral phenotypes. the prese ...201627359102
elevated virulence of an emerging viral genotype as a driver of honeybee loss.emerging infectious diseases (eids) have contributed significantly to the current biodiversity crisis, leading to widespread epidemics and population loss. owing to genetic variation in pathogen virulence, a complete understanding of species decline requires the accurate identification and characterization of eids. we explore this issue in the western honeybee, where increasing mortality of populations in the northern hemisphere has caused major concern. specifically, we investigate the importan ...201627358367
a possible role of dna methylation in functional divergence of a fast evolving duplicate gene encoding odorant binding protein 11 in the honeybee.although gene duplication is seen as the main path to evolution of new functions, molecular mechanisms by which selection favours the gain versus loss of newly duplicated genes and minimizes the fixation of pseudo-genes are not well understood. here, we investigate in detail a duplicate honeybee gene obp11 belonging to a fast evolving insect gene family encoding odorant binding proteins (obps). we report that obp11 is expressed only in female bees in rare antennal sensilla basiconica in contrast ...201627358363
macronutrient ratios in pollen shape bumble bee (bombus impatiens) foraging strategies and floral preferences.to fuel their activities and rear their offspring, foraging bees must obtain a sufficient quality and quantity of nutritional resources from a diverse plant community. pollen is the primary source of proteins and lipids for bees, and the concentrations of these nutrients in pollen can vary widely among host-plant species. therefore we hypothesized that foraging decisions of bumble bees are driven by both the protein and lipid content of pollen. by successively reducing environmental and floral c ...201627357683
infections with the sexually transmitted pathogen nosema apis trigger an immune response in the seminal fluid of honey bees (apis mellifera).honey bee (apis mellifera) males are highly susceptible to infections with the sexually transmitted fungal pathogen nosema apis. however, they are able to suppress this parasite in the ejaculate using immune molecules in the seminal fluid. we predicted that males respond to infections by altering the seminal fluid proteome to minimize the risk to sexually transmit the parasite to the queen and her colony. we used itraq isotopic labeling to compare seminal fluid proteins from infected and noninfe ...201727356667
analysis of the differentiation of kenyon cell subtypes using three mushroom body-preferential genes during metamorphosis in the honeybee (apis mellifera l.).the adult honeybee (apis mellifera l.) mushroom bodies (mbs, a higher center in the insect brain) comprise four subtypes of intrinsic neurons: the class-i large-, middle-, and small-type kenyon cells (lkcs, mkcs, and skcs, respectively), and class-ii kcs. analysis of the differentiation of kc subtypes during metamorphosis is important for the better understanding of the roles of kc subtypes related to the honeybee behaviors. in the present study, aiming at identifying marker genes for kc subtype ...201627351839
impact of managed honey bee viruses on wild bees.several viruses found in the western honey bee (apis mellifera) have recently been detected in other bee species, raising the possibility of spill-over from managed to wild bee species. alternatively, these viruses may be shared generalists across flower-visiting insects. here we explore the former hypothesis, pointing out weaknesses in the current evidence, particularly in relation to deformed wing virus (dwv), and highlighting research areas that may help test it. data so far suggest that dwv ...201627351468
absorption properties and effects of caffeic acid phenethyl ester and its p-nitro-derivative on p-glycoprotein in caco-2 cells and rats.caffeic acid phenethyl ester (cape), isolated from honeybee propolis, has pharmacological applications. a synthesized cape derivative, p-nitro-caffeic acid phenethyl ester (cape-no2), showed similar activities with cape. the pharmacological activities of cape and cape-no2 are related to their absorption properties.201627348457
ozone differentially affects perception of plant volatiles in western honey bees.floral scents play a key role in mediating plant-pollinator interactions. volatile organic compounds (vocs) emitted by flowers are used by flower visitors as olfactory cues to locate flowers, both from a distance and at close range. more recently it has been demonstrated that reactive molecules such as ozone can modify or degrade vocs, and this may impair the communication between plants and their pollinators. however, it is not known whether such reactive molecules also may affect the olfactory ...201627344162
plant acoustics: in the search of a sound mechanism for sound signaling in plants.being sessile, plants continuously deal with their dynamic and complex surroundings, identifying important cues and reacting with appropriate responses. consequently, the sensitivity of plants has evolved to perceive a myriad of external stimuli, which ultimately ensures their successful survival. research over past centuries has established that plants respond to environmental factors such as light, temperature, moisture, and mechanical perturbations (e.g. wind, rain, touch, etc.) by suitably m ...201627342223
reverse micellar extraction of papain with cationic detergent based system: an optimization approach.in this study, reverse micellar extraction of papain model system was performed using cetyltrimethylammonium bromide (ctab)/iso-octane/hexanol/butanol system to optimize the forward and back extraction efficiency (bee). a maximum forward extraction efficiency of 55.0, 61.0, and 54% was achieved with an aqueous phase ph of 11.0, 150 mm ctab/iso-octane and 0.1 m nacl, respectively. taguchi's orthogonal array was applied to optimize the ph of stripping phase, concentration of isopropyl alcohol (ipa ...201727341632
high-dose bee venom exposure induces similar tolerogenic b-cell responses in allergic patients and healthy beekeepers.the involvement of b cells in allergen tolerance induction remains largely unexplored. this study investigates the role of b cells in this process, by comparing b-cell responses in allergic patients before and during allergen immunotherapy (ait) and naturally exposed healthy beekeepers before and during the beekeeping season.201727341567
social polymorphism in the sweat bee lasioglossum (evylaeus) calceatum.temperate-zone socially polymorphic sweat bees (hymenoptera: halictidae) are ideal model systems for elucidating the origins of eusociality, a major evolutionary transition. bees express either social or solitary behaviour in different parts of their range, and social phenotype typically correlates with season length. despite their obvious utility, however, socially polymorphic sweat bees have received relatively little attention with respect to understanding the origins of eusociality. lasioglo ...201727340290
approaches and challenges to managing nosema (microspora: nosematidae) parasites in honey bee (hymenoptera: apidae) colonies.the microsporidia nosema apis (zander) and nosema ceranae (fries) are common intestinal parasites in honey bee (apis mellifera l.) colonies. though globally prevalent, there are mixed reports of nosema infection costs, with some regions reporting high parasite virulence and colony losses, while others201627340190
flight performance of actively foraging honey bees is reduced by a common pathogen.sudden and severe declines in honey bee (apis mellifera) colony health in the us and europe have been attributed, in part, to emergent microbial pathogens, however, the mechanisms behind the impact are unclear. using roundabout flight mills, we measured the flight distance and duration of actively foraging, healthy-looking honey bees sampled from standard colonies, before quantifying the level of infection by nosema ceranae and deformed wing virus complex (dwv) for each bee. neither the presence ...201627337097
crop domestication facilitated rapid geographical expansion of a specialist pollinator, the squash bee peponapis pruinosa.squash was first domesticated in mexico and is now found throughout north america (na) along with peponapis pruinosa, a pollen specialist bee species of the squash genus cucurbita the origin and spread of squash cultivation is well-studied archaeologically and phylogenetically; however, no study has documented how cultivation of this or any other crop has influenced species in mutualistic interactions. we used molecular markers to reconstruct the demographic range expansion and colonization rout ...201627335417
population structuring of the ubiquitous stingless bee tetragonisca angustula in southern brazil as revealed by microsatellite and mitochondrial markers.tetragonisca angustula is one of the most widespread stingless bees in the neotropics. this species swarms frequently and is extremely successful in urban environments. in addition, it is one of the most popular stingless bee species for beekeeping in latin america, so nest transportation and trading is common. nest transportation can change the genetic structure of the host population, reducing inbreeding and increasing homogenization. here, we evaluate the genetic structure of 17 geographic po ...201627334308
efficiency of quechers approach for determining 52 pesticide residues in honey and honey bees.a comparison between quechers and other pesticide extraction procedures for honey and honey bee matrices is discussed. honey bee matrix was extracted by solvent based procedure whereas solid phase extraction was the protocol for the honey matrix. the citrate buffered quechers method was used for both matrices. the methods were evaluated regarding cost (equipment and reagents), time, accuracy, precision, sensitivity and versatility. the results proved that the quechers protocol was the most effic ...201627331025
fat-free mass and calf circumference as body composition indices to determine non-exercise activity thermogenesis in patients with diabetes.to investigate the clinical and anthropometrical parameters that are associated with non-exercise activity thermogenesis that is composed of basal energy expenditure (bee) and diet-induced thermogenesis (dit) in patients with diabetes.201627330721
dichloromethane extracts of propolis protect cell from oxygen-glucose deprivation-induced oxidative stress via reducing apoptosis.bee propolis, a mixture of the secretion from bee tongue gland and wax gland, was collected from the tree bud and barked by bees. the components were rich in terpenes, phenolics, and flavonoids, and had anti-cancer, anti-bacterial, anti-inflammatory, hepatoprotective, and neuroprotection abilities. however, the potential anti-oxidative stress of propolis was not well documented. this study aimed to study the protective effect of propolis on high-incident nonfatal diseases, such as stroke and cer ...201627329777
diversity and human perceptions of bees (hymenoptera: apoidea) in southeast asian megacities.urbanization requires the conversion of natural land cover to cover with human-constructed elements and is considered a major threat to biodiversity. bee populations, globally, are under threat; however, the effect of rapid urban expansion in southeast asia on bee diversity has not been investigated. given the pressing issues of bee conservation and urbanization in southeast asia, coupled with complex factors surrounding human-bee coexistence, we investigated bee diversity and human perceptions ...201627327818
geographic consistency and variation in conflicting selection generated by pollinators and seed predators.floral traits that attract pollinators may also attract seed predators, which, in turn, may generate conflicting natural selection on such traits. although such selection trade-offs are expected to vary geographically, few studies have investigated selection mediated by pollinators and seed predators across a geographic mosaic of environments and floral variation.201627325896
engaging indigenous and academic knowledge on bees in the amazon: implications for environmental management and transdisciplinary research.this paper contributes to the development of theoretical and methodological approaches that aim to engage indigenous, technical and academic knowledge for environmental management. we present an exploratory analysis of a transdisciplinary project carried out to identify and contrast indigenous and academic perspectives on the relationship between the africanized honey bee and stingless bee species in the brazilian amazon. the project was developed by practitioners and researchers of the institut ...201627324644
development of multiple polymorphic microsatellite markers for ceratina calcarata (hymenoptera: apidae) using genome-wide analysis.the small carpenter bee, ceratina calcarata (robertson), is a widespread native pollinator across eastern north america. the behavioral ecology and nesting biology of c. calcarata has been relatively well-studied and the species is emerging as a model organism for both native pollinator and social evolution research. c. calcarata is subsocial: reproductively mature females provide extended maternal care to their brood. as such, studies of c. calcarata may also reveal patterns of relatedness and ...201627324584
fighting off wound pathogens in horses with honeybee lactic acid bacteria.in the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biofilm formation and sometimes to infection. therefore, antibiotics are often prescribed. recent studies have shown that honeybee-specific lactic acid bacteria (lab), involved in honey ...201627324340
activities of venom proteins and peptides with possible therapeutic applications from bees and wasps.the variety of proteins and peptides isolated from honey bee venom and wasp venom includes melittin, adiapin, apamine, bradykinin, cardiopep, mast cell degranulating peptide, mastoparan, phospholipase a2 and secapin. some of the activities they demonstrate may find therapeutic applications.201627323949
weighted global artificial bee colony algorithm makes gas sensor deployment efficient.this paper proposes an improved artificial bee colony algorithm named weighted global abc (wgabc) algorithm, which is designed to improve the convergence speed in the search stage of solution search equation. the new method not only considers the effect of global factors on the convergence speed in the search phase, but also provides the expression of global factor weights. experiment on benchmark functions proved that the algorithm can improve the convergence speed greatly. we arrive at the gas ...201627322262
nitenpyram degradation in finished drinking water.neonicotinoid pesticides and their metabolites have been indicated as contributing factors in the decline of honey bee colonies. a thorough understanding of neonicotinoid toxicity requires knowledge of their metabolites and environmental breakdown products. this work investigated the rapid degradation of the neonicotinoid nitenpyram in finished drinking water.201627321854
atmospheric solids analysis probe mass spectrometry for the rapid identification of pollens and semi-quantification of flavonoid fingerprints.from allergies to plant reproduction, pollens have important impacts on the health of human and plant populations, yet identification of pollen grains remains difficult and time-consuming. low-volatility flavonoids generated from pollens cannot be easily characterized and quantified with current analytical techniques.201627321852
queen pheromone regulates programmed cell death in the honey bee worker ovary.in social insect colonies the presence of a queen, secreting her pheromones, is a key environmental cue for regulating the reproductive state of workers. however, until recently the proximate molecular mechanisms underlying facultative worker sterility were unidentified. studies into worker oogenesis in the honey bee (apis mellifera) have indicated that programmed cell death is central to the regulation of oogenesis. here we investigate how queen pheromone, age of the worker and ovary state affe ...201627321063
[effect of bee venom injection on trka and trpv1 expression in the dorsal root ganglion of rats with collagen-induced arthritis].to investigate the therapeutic effect of acupoint injection of bee venom on collagen-induced arthritis (cia) in rats and explore the mechanism of bee venom therapy in the treatment of rheumatoid arthritis.201627320889
the hexagonal shape of the honeycomb cells depends on the construction behavior of bees.the hexagonal shape of the honey bee cells has attracted the attention of humans for centuries. it is now accepted that bees build cylindrical cells that later transform into hexagonal prisms through a process that it is still debated. the early explanations involving the geometers' skills of bees have been abandoned in favor of new hypotheses involving the action of physical forces, but recent data suggest that mechanical shaping by bees plays a role. however, the observed geometry can arise on ...201627320492
honeybee economics: optimisation of foraging in a variable world.in honeybees fast and efficient exploitation of nectar and pollen sources is achieved by persistent endothermy throughout the foraging cycle, which means extremely high energy costs. the need for food promotes maximisation of the intake rate, and the high costs call for energetic optimisation. experiments on how honeybees resolve this conflict have to consider that foraging takes place in a variable environment concerning microclimate and food quality and availability. here we report, in simulta ...201627320240
innate colour preferences of the australian native stingless bee tetragonula carbonaria sm.innate preferences promote the capacity of pollinators to find flowers. honeybees and bumblebees have strong preferences for 'blue' stimuli, and flowers of this colour typically present higher nectar rewards. interestingly, flowers from multiple different locations around the world independently have the same distribution in bee colour space. currently, however, there is a paucity of data on the innate colour preferences of stingless bees that are often implicated as being key pollinators in man ...201627316718
nectar profitability, not empty honey stores, stimulate recruitment and foraging in melipona scutellaris (apidae, meliponini).in stingless bees (meliponini) like in many other eusocial insect colonies food hoarding plays an important role in colony survival. however, very little is known on how meliponini, a taxon restricted to tropical and subtropical regions, respond to different store conditions. we studied the impact of honey removal on nectar foraging activity and recruitment behaviour in melipona scutellaris and compared our results with studies of the honey bee apis mellifera. as expected, foraging activity incr ...201627316717
pop star finds record of relative's midwife past.when bee gees legend robin gibb began investigating his family tree, he probably did not expect the search to involve a case of midwifery misconduct.201127315999
resources or landmarks: which factors drive homing success in tetragonula carbonaria foraging in natural and disturbed landscapes?to date, no study has investigated how landscape structural (visual) alterations affect navigation and thus homing success in stingless bees. we addressed this question in the australian stingless bee tetragonula carbonaria by performing marking, release and re-capture experiments in landscapes differing in habitat homogeneity (i.e., the proportion of elongated ground features typically considered prominent visual landmarks). we investigated how landscape affected the proportion of bees and nect ...201627311817
synergistic effect of honey and propolis on cutaneous wound healing in rats.accelerating wound healing is now considered as a principle clinical treatment and increasing the quality and speed of healing which has always been emphasized by the scientists. propolis and honey are natural bee products with wide range of biological and medicinal properties. this study was aimed to determine the synergistic effect of honey and propolis in wound healing of rat skin. a total of 75 wistar rats weighing 200-250 gr were placed under general anesthesia and sterile conditions. then ...201627309263
evidence of trapline foraging in honeybees.central-place foragers exploiting floral resources often use multi-destination routes (traplines) to maximise their foraging efficiency. recent studies on bumblebees have showed how solitary foragers can learn traplines, minimising travel costs between multiple replenishing feeding locations. here we demonstrate a similar routing strategy in the honeybee (apis mellifera), a major pollinator known to recruit nestmates to discovered food resources. individual honeybees trained to collect sucrose s ...201627307487
monoterpenoid-based preparations in beehives affect learning, memory, and gene expression in the bee brain.bees are exposed in their environment to contaminants that can weaken the colony and contribute to bee declines. monoterpenoid-based preparations can be introduced into hives to control the parasitic mite varroa destructor. the long-term effects of monoterpenoids are poorly investigated. olfactory conditioning of the proboscis extension reflex (per) has been used to evaluate the impact of stressors on cognitive functions of the honeybee such as learning and memory. the authors tested the per to ...201727306119
genetic variability in captive populations of the stingless bee tetragonisca angustula.low genetic variability has normally been considered a consequence of animal husbandry and a major contributing factor to declining bee populations. here, we performed a molecular analysis of captive and wild populations of the stingless bee tetragonisca angustula, one of the most commonly kept species across south america. microsatellite analyses showed similar genetic variability between wild and captive populations however, captive populations showed lower mitochondrial genetic variability. m ...201627305916
why do varroa mites prefer nurse bees?the varroa mite, varroa destructor, is an acarine ecto-parasite on apis mellifera. it is the worst pest of apis mellifera, yet its reproductive biology on the host is not well understood. in particular, the significance of the phoretic stage, when mites feed on adult bees for a few days, is not clear. in addition, it is not clear whether the preference of mites for nurses observed in the laboratory also happens inside real colonies. we show that varroa mites prefer nurses over both newly emerged ...201627302644
chemical reaction optimization for solving shortest common supersequence problem.shortest common supersequence (scs) is a classical np-hard problem, where a string to be constructed that is the supersequence of a given string set. the scs problem has an enormous application of data compression, query optimization in the database and different bioinformatics activities. due to np-hardness, the exact algorithms fail to compute scs for larger instances. many heuristics and meta-heuristics approaches were proposed to solve this problem. in this paper, we propose a meta-heuristic ...201627299980
royal jelly from apis cerana japonica and apis mellifera.the chemical composition and the properties of protein in royal jellies collected from apis cerana japonica and apis mellifera were respectively analyzed. a. cerana royal jelly (crj) contained more protein and less carbohydrate than a. mellifera royal jelly (mrj). the water-soluble proteins were analyzed by electrophoresis and immunologically. the major protein components of crj and mrj had different molecular weights, isoelectric points, and immunological characteristics.199627299558
pathogenesis of varroosis at the level of the honey bee (apis mellifera) colony.the parasitic mite varroa destructor, in interaction with different viruses, is the main cause of honey bee colony mortality in most parts of the world. here we studied how effects of individual-level parasitization are reflected by the bee colony as a whole. we measured disease progression in an apiary of 24 hives with differing degree of mite infestation, and investigated its relationship to 28 biometrical, physiological and biochemical indicators. in early summer, when the most heavily infest ...201627296894
a comparative genomic analysis of targets of hox protein ultrabithorax amongst distant insect species.in the fruitfly drosophila melanogaster, the differential development of wing and haltere is dependent on the function of the hox protein ultrabithorax (ubx). here we compare ubx-mediated regulation of wing patterning genes between the honeybee, apis mellifera, the silkmoth, bombyx mori and drosophila. orthologues of ubx are expressed in the third thoracic segment of apis and bombyx, although they make functional hindwings. when over-expressed in transgenic drosophila, ubx derived from apis or b ...201627296678
[bilateral radiofrequency anterior thalamotomy in intractable epilepsy patients].identification of the crucial role of the anterior thalamic nuclei (atn) in the generalization of seizures led to increased interest in surgical interventions in this particular area in intractable epilepsy patients. simulation of atn destruction in animals demonstrated its high efficacy for both preventing the seizure development and reducing the seizure rate. however, bilateral radiofrequency destruction of the anterior thalamic nuclei in humans has not yet bee described.201727296535
getting useful information from rna-seq contaminants: a case of study in the oil-collecting bee tetrapedia diversipes transcriptome. 201627295584
selective behaviour of honeybees in acquiring european propolis plant precursors.honey bees harvest resins from various plant species and use them in the hive as propolis. while there have been a number of studies concerning the chemical composition of this antimicrobial product, little is known about selective behavior and bee preference when different potential plant sources of resin are available. the main objective of this paper was to investigate some aspects of behavioral patterns of honeybees in the context of resin acquisition. samples of propolis originating from te ...201627294416
bee pollen as a promising agent in the burn wounds treatment.the aim of the present study was to visualize the benefits and advantages derived from preparations based on extracts of bee pollen as compared to pharmaceuticals commonly used in the treatment of burns. the bee pollen ointment was applied for the first time in topical burn treatment. experimental burn wounds were inflicted on two white, domestic pigs. clinical, histopathological, and microbiological assessment of specimens from burn wounds, inflicted on polish domestic pigs, treated with silver ...201627293466
a new modified artificial bee colony algorithm with exponential function adaptive steps.as one of the most recent popular swarm intelligence techniques, artificial bee colony algorithm is poor at exploitation and has some defects such as slow search speed, poor population diversity, the stagnation in the working process, and being trapped into the local optimal solution. the purpose of this paper is to develop a new modified artificial bee colony algorithm in view of the initial population structure, subpopulation groups, step updating, and population elimination. further, dependin ...201627293426
cloning and functional expression of intracellular loop variants of the honey bee (apis mellifera) rdl gaba receptor.the insect gaba receptor, rdl (resistance to dieldrin), plays central roles in neuronal signalling and is the target of several classes of insecticides. to study the gaba receptor from an important pollinator species, we cloned rdl cdna from the honey bee, apis mellifera. three rdl variants were identified, arising from differential use of splice acceptor sites in the large intracellular loop between transmembrane regions 3 and 4. these variants were renamed from previously, as amel_rdlvar1, ame ...201627288983
chemical characterization, antioxidant, anti-inflammatory and cytotoxic properties of bee venom collected in northeast portugal.bee venom (bv) or apitoxin is a complex mixture of substances with reported biological activity. in the present work, five bee venom samples obtained from apis mellifera iberiensis from the northeast portugal (two different apiaries) were chemically characterized and evaluated for their antioxidant, anti-inflammatory and cytotoxic properties. the lc/dad/esi-ms(n) analysis of the samples showed that melittin was the most abundant compound, followed by phospholipase a2 and apamin. all the samples ...201627288930
honeybees produce millimolar concentrations of non-neuronal acetylcholine for breeding: possible adverse effects of neonicotinoids.the worldwide use of neonicotinoid pesticides has caused concern on account of their involvement in the decline of bee populations, which are key pollinators in most ecosystems. here we describe a role of non-neuronal acetylcholine (ach) for breeding of apis mellifera carnica and a so far unknown effect of neonicotinoids on non-target insects. royal jelly or larval food are produced by the hypopharyngeal gland of nursing bees and contain unusually high ach concentrations (4-8 mm). ach is extreme ...201627285384
genome sequence of hafnia alvei bta3_1, a bacterium with antimicrobial properties isolated from honey bee gut.hafnia alvei bta3_1, a strain with antibacterial properties, was isolated from honey bee gut and cultured under aerobic and anaerobic conditions. to explore the potential genetic bases of its antibacterial and possible pathogenic properties, the complete genome of this organism was sequenced and analyzed.201627284146
multiclass method for pesticides quantification in honey by means of modified quechers and uhplc-ms/ms.bee products can be produced in an environment contaminated by pesticides that can be transported by honey bees to the hive and incorporated into the honey. therefore, rapid and modern methods to determine pesticide residues in honey samples are essential to guarantee consumers' health. in this study, a simple multiresidue method for the quantification of 116 pesticides in honey is proposed. it involves the use of a modified quechers procedure followed by uhplc-ms/ms analysis. the method was val ...201627283616
[new approach to the mitotype classification in black honeybee apis mellifera mellifera and iberian honeybee apis mellifera iberiensis].the black honeybee apis mellifera mellifera l. is today the only subspecies of honeybee which is suitable for commercial breeding in the climatic conditions of northern europe with long cold winters. the main problem of the black honeybee in russia and european countries is the preservation of the indigenous gene pool purity, which is lost as a result of hybridization with subspecies, a. m. caucasica, a. m. carnica, a. m. carpatica, and a. m. armeniaca, introduced from southern regions. genetic ...201627281852
specific synergist for neonicotinoid insecticides: ippa08, a cis-neonicotinoid compound with a unique oxabridged substructure.insecticide synergists are key components to increase the control efficacy and reduce active ingredient use. here, we describe a novel insecticide synergist with activity specific for insecticidal neonicotinoids. the synergist ippa08, a cis configuration neonicotinoid compound with a unique oxabridged substructure, could increase the toxicity of most neonicotinoid insecticides belonging to the insecticide resistance action committee (irac) 4a subgroup against a range of insect species, although ...201627281691
honey bee (apis mellifera) queen reproductive potential affects queen mandibular gland pheromone composition and worker retinue response.reproductive division of labor is one of the defining traits of honey bees (apis mellifera), with non-reproductive tasks being performed by workers while a single queen normally monopolizes reproduction. the decentralized organization of a honey bee colony is maintained in large part by a bouquet of queen-produced pheromones, the distribution of which is facilitated by contact among workers throughout the hive. previous studies have shown that the developmental fate of honey bee queens is highly ...201627281328
correction: honey bee inhibitory signaling is tuned to threat severity and can act as a colony alarm signal.[this corrects the article doi: 10.1371/journal.pbio.1002423.].201627280715
identification of multiple loci associated with social parasitism in honeybees.in colonies of the honeybee apis mellifera, the queen is usually the only reproductive female, which produces new females (queens and workers) by laying fertilized eggs. however, in one subspecies of a. mellifera, known as the cape bee (a. m. capensis), worker bees reproduce asexually by thelytoky, an abnormal form of meiosis where two daughter nucleii fuse to form single diploid eggs, which develop into females without being fertilized. the cape bee also exhibits a suite of phenotypes that faci ...201627280405
antifungal activity of bee venom and sweet bee venom against clinically isolated candida albicans.the purpose of this study was to investigate the antifungal effect of bee venom (bv) and sweet bee venom (sbv) against candida albicans (c. albicans) clinical isolates.201627280049
endosymbiotic bacteria in honey bees: arsenophonus spp. are not transmitted transovarially.intracellular endosymbiotic bacteria are common and can play a crucial role for insect pathology. therefore, such bacteria could be a potential key to our understanding of major losses of western honey bees (apis mellifera) colonies. however, the transmission and potential effects of endosymbiotic bacteria in a. mellifera and other apis spp. are poorly understood. here, we explore the prevalence and transmission of the genera arsenophonus, wolbachia, spiroplasma and rickettsia in apis spp. colon ...201627279628
virion structure of iflavirus slow bee paralysis virus at 2.6-angstrom resolution.the western honeybee (apis mellifera) is the most important commercial insect pollinator. however, bees are under pressure from habitat loss, environmental stress, and pathogens, including viruses that can cause lethal epidemics. slow bee paralysis virus (sbpv) belongs to the iflaviridae family of nonenveloped single-stranded rna viruses. here we present the structure of the sbpv virion determined from two crystal forms to resolutions of 3.4 å and 2.6 å. the overall structure of the virion resem ...201627279610
multiple functions of volatiles in flowers and leaves of elsholtzia rugulosa (lamiaceae) from southwestern china.although the roles of volatile compounds have been examined separately in plant-herbivore or plant-pollinator interactions, few studies have focused on how plant scents can attract effective pollinators, repel ineffective pollinators, and defend against attacks by insect herbivores. we explored the functional significance of volatile compounds that impart a strong odor to elsholtzia rugulosa, a shrub species in southwestern china. we monitored the pollinating honey bee apis cerana, as well as tw ...201627278713
deceived by orchids: sex, science, fiction and darwin.between 1916 and 1927, botanists in several countries independently resolved three problems that had mystified earlier naturalists - including charles darwin: how did the many species of orchid that did not produce nectar persuade insects to pollinate them? why did some orchid flowers seem to mimic insects? and why should a native british orchid suffer 'attacks' from a bee? half a century after darwin's death, these three mysteries were shown to be aspects of a phenomenon now known as pseudocopu ...201627278105
soldiers in a stingless bee.the differentiation of workers into morphological subcastes (e.g., soldiers) represents an important evolutionary transition and is thought to improve division of labor in social insects. soldiers occur in many ant and termite species, where they make up a small proportion of the workforce. a common assumption of worker caste evolution is that soldiers are behavioral specialists. here, we report the first test of the "rare specialist" hypothesis in a eusocial bee. colonies of the stingless bee t ...201627277408
sucrose sensitivity of honey bees is differently affected by dietary protein and a neonicotinoid pesticide.over a decade, declines in honey bee colonies have raised worldwide concerns. several potentially contributing factors have been investigated, e.g. parasites, diseases, and pesticides. neonicotinoid pesticides have received much attention due to their intensive use in crop protection, and their adverse effects on many levels of honey bee physiology led the european union to ban these compounds. due to their neuronal target, a receptor expressed throughout the insect nervous system, studies have ...201627272274
generalist behavior describes pollen foraging for perceived oligolectic and polylectic bees.native bees provide essential pollination services to cultivated and wild plants worldwide. despite the need to conserve pollinators, the foraging patterns of native bees are poorly understood. classic concepts of resource use have typically categorized bee species as specialists or generalists based on floral visitation patterns. while intraspecific variation in bee foraging likely depends on local land use, sex, and phenological period, among other factors, these potential drivers of floral vi ...201627271950
the effects of crop intensification on the diversity of native pollinator communities.increases in agricultural conversion are leading to declines in native grasslands and natural resources critical for beneficial insects. however, little is known regarding how these changes affect pollinator diversity. land use types were categorized within 300 m and 3 km radii of pollinator sampling locations in brookings county, sd. pollinator abundance and species richness were regressed on the proportion of the landscape dedicated to row crops, grass and pasture, forage crops, small grains, ...201627271948
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