Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| opisthorchis viverrini: relationships between egg counts, worms recovered and antibody levels within an endemic community in northeast thailand. | three techniques for estimating the intensity of opisthorchis viverrini infection in individuals from a northeast thai community are compared. egg counts were determined using a quantitative formalin/ethyl acetate technique, worm burdens were estimated by expulsion chemotherapy and antibody levels were measured by elisa. log-transformed worm and egg counts were closely correlated (r = 0.80), suggesting that both measurements provide good assessments of relative intensity of infection. however, n ... | 1991 | 1852494 |
| relationship between faecal egg count and worm burden of opisthorchis viverrini in human autopsy cases. | the relationship between faecal examination for egg output and worm burden of opisthorchis viverrini in man of 181 autopsy cases from northeast thailand is described. diagnosis of the parasite infection by stool examination for the presence of eggs was less sensitive than the worm recovery technique. using stoll's dilution and formalin-ether technique, no eggs were detected in the faeces of 20 cases harbouring low worm burdens (less than 20 worms). the quantitative faecal egg count by stoll's di ... | 1991 | 1852493 |
| current status of food-borne parasitic zoonoses in laos. | stool samples from a total of 1,008 persons were examined for intestinal parasites during a comprehensive study on the epidemiology of small fluke infections in laos. the prevalence of small fluke eggs in the stool was seen to peak at age 20 years, particularly in men from villages (90.4%). three quarters of infections belong to the category of light infections and only 0.6% to the category of very heavy according to eggs per gram of feces (epg). the highest epg was in the 11-15 year age group. ... | 1991 | 1822938 |
| preliminary estimates of economic impact of liver fluke infection in thailand and the feasibility of irradiation as a control measure. | liver fluke infection by opisthorchis viverrini is the leading cause of food-borne parasitic disease in thailand. approximately one third of the population in the northeastern region of the country, ie, 6-7 million, are infected by this parasite through the habit of consuming raw or insufficiently cooked freshwater fish, especially those of cyprinoid family. a recent survey showed that 60% of the work force in the northeast between the age of 15 and 60 is infected. the estimated wage loss of thi ... | 1991 | 1822933 |
| immunodiagnosis of opisthorchiasis. | monoclonal antibody-based enzyme-linked immunosorbent assay and dna dot blot hybridization techniques were developed and evaluated for their potential in the detection of opisthorchis viverrini. a mixture of igg monoclonal antibodies specific for the 89 kda metabolic product of o. viverrini was captured on a microtiter plate by rabbit anti-mouse igg and used in a sandwich elisa for the detection of soluble parasite antigen in the feces of patients with opisthorchiasis. as little as 0.1 ng of the ... | 1991 | 1822882 |
| serological differentiation of human small fluke infections using opisthorchis viverrini and haplorchis taichui antigens. | sera from 642 inhabitants of vientiane province (laos) were examined by enzyme-linked immunosorbent assay (elisa) using cytoplasmic and membranous antigens prepared from adult worms. worms of opisthorchis viverrini originated from liver of dissected cats, haplorchis taichui were obtained from a stool specimen of a laotian patient after praziquantel treatment. the sera were divided into five groups according to the intensity of infection expressed as egg count per gram of patients stool (epg). co ... | 1991 | 1822881 |
| differential diagnosis of opisthorchiid and heterophyid metacercariae (trematoda) infecting flesh of cyprinid fish from nam ngum dam lake in laos. | the examination of flesh of 313 cyprinid fish from the nam ngum water reservoir in vientiane province, laos, revealed the presence of four morphologically similar opisthorchiid and heterophyid metacercariae of medical importance. the following morphological characters, visible under dissecting microscope with low magnification, were typical of trematodes recorded in fish: (1) opisthorchis viverrini, the liver fluke, has oval cysts containing quickly moving c-shaped metacercariae provided with a ... | 1991 | 1822880 |
| eggshell morphology of the small eggs of human trematodes in thailand. | light and scanning electron micrographs of opisthorchis viverrini, haplorchis taichui, h. pumilio, a phaneropsolus bonnei, and prosthodendrium molenkampi eggs were studied. under light microscopy. o. viverrini eggs had rough eggshells and prominent shoulders. h. taichui, h. pumilio were similar in shape and had smooth eggshells and prominent shoulders. h. pumilio eggs were bigger than h. taichui eggs. p. bonnei and p. molenkampi eggs had smooth eggshells and indistinct shoulders. p. bonnei eggs ... | 1991 | 1820654 |
| comparative studies on the morphology of the eggs of opisthorchis viverrini and lecithodendriid trematodes. | iodine staining and scanning electron microscopy were used to determine the morphological features distinguishing opisthorchis viverrini from lecithodendriid eggs in human feces. the embryonated eggs of lecithodendriid trematodes differ from o. viverrini by the presence of an iodophilic body, a large mass at the posterior end of miracidium that stains brown in 0.2% iodine solution and the curved miracidium of phaneropsolus bonnei. all forms of lecithodendriid eggs can be differentiated from thos ... | 1991 | 1820653 |
| quantitative post-mortem study of opisthorchis viverrini in man in north-east thailand. | the results of an autopsy study of opisthorchis viverrini infection in 181 accident subjects in northeast thailand are presented. the age-prevalence profiles were similar whether determined by faecal examination for parasite eggs or by recovery of worms from the liver. the prevalence increased rapidly with age and reached a plateau at 70-80% in adults. the overall prevalence estimated by faecal examination was 69.2%, while that measured by worm recovery was 79.2%. the average intensity of infect ... | 1991 | 1801350 |
| specific monoclonal antibodies to opisthorchis viverrini. | a balb/c mouse was immunized with a crude soluble antigen of opisthorchis viverrini adult worms (ovaa) over a period of 7 months. spleen cells from the immune mouse were fused with sp2/0 myeloma cells. among the 264 tissue culture wells containing the fused cells, cells of 96 wells (36%) produced antibodies to the immunizing agent. antibodies produced by cells in several wells reacted with antigens from other species of parasite. cells of 17 wells produced antibodies specific only to ovaa, thus ... | 1991 | 1787041 |
| isolation and characterization of opisthorchis viverrini specific dna probe. | an opisthorchis viverrini specific repetitive dna fragment was isolated from the o. viverrini genome. the cloned fragment, herein designated pov-a6, contained tandemly repeated 334 bp o. viverrini dna and has the potential to be a highly specific and sensitive dna probe. radioactively labelled pov-a6 could detect as little as 25 pg of its own genomic dna and five purified eggs by dot-blot hybridization. the presence of 50 o. viverrini eggs could be readily detected in samples containing faecal m ... | 1991 | 1779978 |
| molecular analysis of t and b cell repertoires in mice immunized with opisthorchis viverrini antigens. | b10 mice were immunized with an opisthorchis viverrini somatic extract and then their responses were analyzed. the antigenic fractions of the extract were separated by sds-polyacrylamide gel electrophoresis, electroblotted to nitrocellulose membranes and solubilized for use in lymphocyte culture. antibody specificity was also visualized by immunoblotting using immunized mouse sera. the mr of the main immunogenic fractions for t cells ranged from 28 to 46 kda, whereas those recognized by antibodi ... | 1991 | 1757200 |
| human opisthorchis and haplorchis infections in laos. | a study of the epidemiology of trematode infections was carried out in vientiane province, laos, comprising examination of snails, fish, cats, and human stools. opisthorchis viverrini cercariae were found in the snail bithynia siamensis goniomphalus (bithyniidae) and haplorchis taichui cercariae in tarebia granifera (thiaridae). o. viverrini metacercariae were recorded in the flesh of 7 species of cyprinid fish; metacercariae of the heterophyid fluke haplorchis taichui were found in 4 species of ... | 1991 | 1755066 |
| repeated exposure to opisthorchis viverrini and treatment with the antihelminthic praziquantel lacks carcinogenic potential. | the effects of repeated praziquantel administration, subsequent to infection and reinfection with opisthorchis viverrini (ov), on lesion development in the syrian hamster liver were investigated. five applications of the antihelminthic drug were made (300 mg/kg body wt, i.g.), each time approximately 5 weeks after dosing with 60-80 ov metacercariae at weeks 0, 8, 16, 24 and 32. the animals were then maintained until week 40 when they were killed; histopathological investigation revealed no signi ... | 1992 | 1740024 |
| production and characterization of monoclonal antibodies against the excretory-secretory antigen of the liver fluke (opisthorchis viverrini). | monoclonal antibodies (moab) were produced against a major soluble metabolic product (excretory-secretory, es) of opisthorchis viverrini. the latter was obtained in a form of spent culture medium in which the adult flukes had been maintained in vitro. the moab produced were exclusively associated with either igg or igm isotypes. when screened against a panel of parasite antigens by indirect elisa, these moab exhibited three patterns of reactivity. approximately 50% of the moab were highly specif ... | 1991 | 1717391 |
| multistage carcinogenesis of liver-fluke-associated cholangiocarcinoma in thailand. | in northeast thailand, the traditional habit of eating ground, raw freshwater and salt-fermented fish on a daily basis results in a local population repeatedly exposed to both liver fluke (opisthorchis viverrini) infection and consuming nitrosamine-contaminated food from early in life. epidemiological studies have revealed a coincident high prevalence of cholangiocarcinoma in this region and we have demonstrated in animal models that dietary contamination with nitrosamines and opisthorchiasis ar ... | 1991 | 1668894 |
| a histopathologic study of 61 cases of peripheral intrahepatic cholangiocarcinoma. | the histopathological study of 61 cases of peripheral intrahepatic cholangiocarcinoma was reported. of the 5 autopsied livers and 13 surgically resected livers, 89 per cent showed massive gross appearance, 94 per cent contained opisthorchis viverrini or showed evidence of previous opisthorchiasis. gall stones were found in 7 cases (11.5%), common bile duct stones were found in one case. the tumors were classified into well differentiated, less differentiated and uncommon types (adenosquamous, an ... | 1991 | 1665850 |
| the role of infection by opisthorchis viverrini, hepatitis b virus, and aflatoxin exposure in the etiology of liver cancer in thailand. a correlation study. | the incidence of the two principle types of liver cancer (hepatocellular carcinoma and cholangiocarcinoma) in five different areas of thailand was compared with the prevalence of exposure to the main risk factors in samples of the population. cholangiocarcinoma showed striking variations in incidence, which correlated closely with markers of exposure to the liver fluke, opisthorchis viverrini. however, there was little geographic variation in incidence of hepatocellular carcinoma or in prevalenc ... | 1991 | 1657355 |
| opisthorchis viverrini infestation and endogenous nitrosamines as risk factors for cholangiocarcinoma in thailand. | cholangiocarcinoma (cca) is one of the most common cancers in north-east thailand and has been associated with infestation by the liver fluke opisthorchis viverrini (ov). two samples of 12-hr overnight urine (after dosing with proline and ascorbic acid or proline alone) were collected from 20 inhabitants from each of 5 contrasting incidence areas for cca. the incidence of cca was not correlated with either the amount of npro or other nitrosamino acids, endogenous nitrosation potential (differenc ... | 1991 | 1650329 |
| endogenous nitrosamines and liver fluke as risk factors for cholangiocarcinoma in thailand. | cholangiocarcinoma (cca) is one of the most prevalent cancers in north-east thailand and has been associated with infestation by the liver fluke opisthorchis viverrini (ov). two samples of 12-h overnight urine (after dosing with 500 mg proline and 200 mg ascorbic acid or 500 mg proline alone) were collected from about 100 inhabitants in five contrasting incidence areas for cca and hepatocellular carcinoma. the incidences of cca and hepatocellular carcinoma were not correlated with either the amo ... | 1991 | 1649794 |
| liver cancer in thailand. i. a case-control study of cholangiocarcinoma. | potential risk factors for cholangiocarcinoma were investigated in a case-control study among inhabitants of north-east thailand, which included 103 cases from 3 hospitals, with age- and sex-matched controls. a clear association with past or present infection with opisthorchis viverrini, as indicated by raised serum antibodies, was found (o.r. 5.0), and at least two-thirds of cases can be attributed to this cause. the results suggest that males may be at higher risk than females. there was no as ... | 1991 | 1645697 |
| detection of opisthorchis viverrini antigens in stools using specific monoclonal antibody. | detection of opisthorchis viverrini antigens in stools using specific monoclonal antibody. international journal for parasitology 22: 527-531. a sandwich enzyme-linked immunosorbent assay (elisa) was developed for detecting opisthorchis viverrini antigen in faecal extracts of four groups of individuals. these were 24 patients with o. viverrini infection only (group 1), 31 patients with o. viverrini and other parasitic infections (group 2), 141 patients with other parasitic infections (group 3) a ... | 1992 | 1644529 |
| comparative morphology of eggs of the haplorchiinae (trematoda: heterophyidae) and some other medically important heterophyid and opisthorchiid flukes. | the egg morphology of the following medically important small flukes from southeast asia and far east were studied: opisthorchis viverrini, clonorchis sinensis (opisthorchiidae), haplorchis taichui, h. pumilio, h. yokogawai, stellantchasmus falcatus and metagonimus sp. (heterophydiae). this study revealed a great intraspecific variability and interspecific similarity in size and shape of eggs. the eggs shape does not seem to be suitable for species identification. on the other hand, biometrical ... | 1992 | 1644359 |
| separation and characterization of adult worm proteins and glycoproteins from the liver fluke opisthorchis viverrini. | detailed studies of liver fluke proteins and antigens are necessary to facilitate further investigation of the human immune responses to these parasites. accordingly, opisthorchis viverrini antigens were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and immunoblotting. we initially encountered excessive background smearing, vertical streaking, and indistinct bands that were similar to problems previously described by investigators studying this and other tremat ... | 1992 | 1635026 |
| cloning and characterization of ribosomal rna genes from opisthorchis viverrini. | the ribosomal dna (rdna) unit of the liver fluke opisthorchis viverrini has been cloned and characterized. the results demonstrated that the total length of this unit is approximately 13 kb, containing 4.2 kb of large subunit (lsu) rdna, 2.0 kb of small subunit (ssu) rdna, 1.0 kb of transcribed spacer dna and 5.8 kb of non-transcribed + external transcribed spacer dna. examination of the non-transcribed spacer region between different rdna units showed variation in the restriction sites rather t ... | 1992 | 1594296 |
| control of opisthorchis viverrini cercariae using the copepod mesocyclops leuckarti. | 1992 | 1439992 | |
| malacological survey in the sirikit reservoir, the largest earthfilled dam in thailand. | ten species of freshwater molluscs were found in a malacological survey in the sirikit reservoir in 1985. among these species, tricula aperta or neotricula aperta, intermediate host of human schistosomes, bithynia (digoniostoma) siamensis goniomphalos and b. (d.) funiculata, the first intermediate host of opisthorchis viverrini were not found. it is revealed that most of the habitats in the sirikit reservoir are not suitable for the survival and colonization of molluscs. thus few species of edib ... | 1992 | 1439988 |
| development of immune-complex glomerulonephritis and amyloidosis in syrian golden hamsters infected with opisthorchis viverrini. | renal disease associated with opisthorchis viverrini infection was investigated in syrian golden hamsters. on the fourth week after infection with 100 viable metacercariae; anti-tegumental membrane antibodies were detected in the sera by immunofluorescence antibody technic and by enzyme-linked immunosorbent assay. six weeks after infection tegumental and anti-tegumental membrane immune-complex and amyloid fibrils were found in the glomeruli. amyloid was characterized to be aa protein. acute prol ... | 1992 | 1402485 |
| prevalence and intensity of opisthorchis viverrini in rural community near the mekong river on the thai-laos border in northeast thailand. | the prevalence and intensity of opisthorchis viverrini in fourteen villages in nakhon-phanom province, northeast, thailand have been investigated. overall prevalence of o. viverrini infection was 66.4 per cent in a total population of 2,412 individuals. the prevalence was 18.5 per cent in children under 5 years, 38.9 per cent in those aged 5-9 years, and ranged from 64.9 per cent to 82.2 per cent in the age group above 10 years. the intensity of o. viverrini infection increased with age. the mea ... | 1992 | 1402447 |
| cholangiocarcinomas in japanese and thai patients: difference in etiology and incidence of point mutation of the c-ki-ras proto-oncogene. | point-mutational activation of the c-ki-ras proto-oncogene has been shown to be rare in human hepatocellular carcinoma, the most common primary liver cancer and one usually associated with chronic viral infection. to reveal the association of c-ki-ras activation with cholangiocarcinogenesis under different etiological backgrounds, the incidence of point mutation at codons 12 and 13 of the c-ki-ras proto-oncogene was examined in three groups of human liver cancers with differentiation to biliary ... | 1992 | 1336666 |
| histomorphological characteristics of cholangiocellular carcinomas in northeast thailand, where a region infection with the liver fluke, opisthorchis viverrini is endemic. | northeast thailand has a very high incidence rate of intrahepatic biliary tumors which is believed to closely related to infestation with the liver fluke, opisthorchis viverrini. this study was conducted to ascertain whether there are any phenotypic differences in such tumors between northeast thailand and japan, a country free of liver flukes. forty one intrahepatic cholangiocarcinomas from patients in northeast thailand were histopathologically compared with 39 lesions collected in japan. the ... | 1992 | 1334615 |
| opisthorchis viverrini infection in northeast thailand and its relationship to cholangiocarcinoma. | 1992 | 1327263 | |
| relationship between intensity of opisthorchis viverrini infection and hepatobiliary disease detected by ultrasonography. | twenty-four locality-, age- and sex-matched groups of village residents with no light, moderate and heavy opisthorchis viverrini infection were examined by ultrasonography. highly significant differences were observed between the groups in the relative size of the left lobe of the liver and the fasting and post-meal size of the gall-bladder. in addition, indistinct gall-bladder wall, the presence of gall-bladder sludge and strongly enhanced portal vein radicle echoes were most frequently observe ... | 1992 | 1311966 |
| study on the surface morphology of the developmental stages of the liver fluke, opisthorchis viverrini (trematoda: opisthorchiidae). | the external morphology of some developmental stages of the fluke opisthorchis viverrini (trematoda: opisthorchiidae), parasitizing humans in southeast asia was studied for the first time using electron microscopy. the surface structure of the egg, as well as the rediae, cercaria, metacercaria, and adult found in naturally infected hosts from laos are described herein and their morphological characteristics discussed. | 1992 | 1290379 |
| larval stages of medically important flukes (trematoda) from vientiane province, laos. part ii. cercariae. | the cercariae of five flukes, which are potential human parasites, opisthorchis viverrini (family opisthorchiidae), haplorchis taichui (heterophyidae), schistosoma spindale (schistosomatidae), fasciolopsis buski, and fasciola gigantica (fasciolidae) were found in freshwater snails from laos. the cercariae of the above species, recorded in laos for the first time, are illustrated and their morphology is briefly described. the snail helicorbis umbilicalis represents a new intermediate host of f. b ... | 1992 | 1290378 |
| specific and cross-reactive monoclonal antibodies to the 89-kda antigen of opisthorchis viverrini. | 1992 | 1283231 | |
| socio-economic, health and nutritional status of the villagers in the nong wai irrigation area, khon kaen, northeast thailand. | studies were carried out from june 1974 to may 1975 on the socio-economic status, health and nutritional status of the people in 4 villages, in the irrigation area of the nong wai pioneer agricultural project of khon kaen province, northeast thailand. the result obtained were compared with those in 2 non-irrigated villages in the same province, in order to identify the health and nutritional problems which might arise during the water resource development in the irrigation area. it was found tha ... | 1976 | 1030856 |
| liver changes in hamsters infected with a liver fluke of man, opisthorchis viverrini. | thirty male syrian golden hamsters were each infected with 100 metacercariae of opisthorchis viverrini. the hamsters were killed at 3, 7, 15, 30, 154 days of infection. the early pathological changes consisted of an acute inflammatory reaction involving the bile ducts of the second order and the portal connective tissue, especially the large veins, as well as focal coagulation necrosis of the liver lobules. as the flukes developed into adults they induced hyperplasia and adenomatous formations o ... | 1978 | 686245 |
| effects of the injectable contraceptive depot medroxyprogesterone acetate in thai women with liver fluke infestation: results after six months. | the effect of the three-monthly injectable contraceptive depot medroxyprogesterone acetate (dmpa) on liver and lipid function was assessed in thai women with liver fluke (opisthorchis viverrini) infestation, dmpa administration being started in the immediate postpartum period. immediate postpartum iud and sterilization acceptors with fluke infestation were recruited as a comparison (control) group for the fluke-positiv dmpa acceptors. comparable groups of fluke-negative acceptors were recruited ... | 1977 | 302157 |
| effects of dimethylnitrosamine on induction of cholangiocarcinoma in opisthorchis viverrini-infected syrian golden hamsters. | 1978 | 214229 |