Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| candida arteritis occurring in a liver transplant recipient. | we report the first case, to our knowledge, of candida arteritis in a liver transplant recipient. the patient presented with hemorrhagic shock requiring emergency arterial repair. as candida albicans, candida tropicalis, and candida glabrata were growing in the arterial tissue, the patient received antifungal therapy for 5 months, but died because of chronic graft dysfunction. no evidence of fungal infection was found in the tissue on postmortem examination. | 2014 | 24750364 |
| evolution of bacterial consortia in spontaneously started rye sourdoughs during two months of daily propagation. | the evolution of bacterial consortia was studied in six semi-solid rye sourdoughs during long-term backslopping at different temperatures. each rye sourdough was started spontaneously in a laboratory (dough yield 200), propagated at either 20°c or 30°c, and renewed daily at an inoculation rate of 1∶10 for 56 days. the changes in bacterial diversity over time were followed by both dgge coupled with partial 16s rrna gene sequencing and pyrosequencing of bar-coded 16s rrna gene amplicons. four spec ... | 2014 | 24748058 |
| [place of vulvovaginal candidiasis in the lower genital tract infections and associated risk factors among women in benin]. | determine the place of vulvo-vaginal candidiasis (vvc) in the lower genital infections and seek risk factors among women in benin. | 2014 | 24746421 |
| in vitro antifungal susceptibility of candida glabrata to caspofungin and the presence of fks mutations correlate with treatment response in an immunocompromised murine model of invasive infection. | it has been argued that the in vitro activity of caspofungin (csp) is not a good predictor of the outcome of echinocandin treatment in vivo. we evaluated the in vitro activity of csp and the presence of fks mutations in the hot spot 1 (hs1) region of the fks1 and fks2 genes in 17 candida glabrata strains with a wide range of mics. the efficacy of csp against systemic infections from each of the 17 strains was evaluated in a murine model. no hs1 mutations were found in the eight strains showing m ... | 2014 | 24733474 |
| a seco-laurane sesquiterpene and related laurane derivatives from the red alga laurencia okamurai yamada. | a ring-cleaved sesquiterpene, named seco-laurokamurone, four laurane-type sesquiterpenes, laurepoxyene, 3β-hydroperoxyaplysin, 3α-hydroperoxy-3-epiaplysin, and 8,10-dibromoisoaplysin, one laurokamurane-type sesquiterpene, laurokamurene d, and one bisabolane-type sesquiterpene, (5s)-5-acetoxy-β-bisabolene, have been isolated from a re-collection of the red alga laurencia okamurai yamada, together with six other known sesquiterpenes. their structures, including relative configuration, were elucida ... | 2014 | 24731260 |
| histidine degradation via an aminotransferase increases the nutritional flexibility of candida glabrata. | the ability to acquire nutrients during infections is an important attribute in microbial pathogenesis. amino acids are a valuable source of nitrogen if they can be degraded by the infecting organism. in this work, we analyzed histidine utilization in the fungal pathogen of humans candida glabrata. hemiascomycete fungi, like c. glabrata or saccharomyces cerevisiae, possess no gene coding for a histidine ammonia-lyase, which catalyzes the first step of a major histidine degradation pathway in mos ... | 2014 | 24728193 |
| multi-drug resistant oral candida species isolated from hiv-positive patients in south africa and cameroon. | candida species are a common cause of infection in immune-compromised hiv-positive individuals, who are usually treated with the antifungal drug, fluconazole, in public hospitals in africa. however, information about the prevalence of drug resistance to fluconazole and other antifungal agents on candida species is very limited. this study examined 128 candida isolates from south africa and 126 cameroonian candida isolates for determination of species prevalence and antifungal drug susceptibility ... | 2014 | 24726686 |
| enhancement of acetoin production in candida glabrata by in silico-aided metabolic engineering. | acetoin is a promising chemical compound that can potentially serve as a high value-added platform for a broad range of applications. many industrial biotechnological processes are moving towards the use of yeast as a platform. the multi-auxotrophic yeast, candida glabrata, can accumulate a large amount of pyruvate, but produces only trace amounts of acetoin. here, we attempted to engineer c. glabrata to redirect the carbon flux of pyruvate to increase acetoin production. | 2014 | 24725668 |
| genome-wide survey of transcriptional initiation in the pathogenic fungus, candida glabrata. | dna sequencing of the 5'-flanking region of the transcriptome effectively identifies transcription initiation sites and also aids in identifying unknown genes. this study describes a comprehensive polling of transcription start sites and an analysis of full-length complementary dnas derived from the genome of the pathogenic fungus candida glabrata. a comparison of the sequence reads derived from a cdna library prepared from cells grown under different culture conditions against the reference gen ... | 2014 | 24725256 |
| isolation of cholesterol-dependent, multidrug-resistant candida glabrata strains from blood cultures of a candidemia patient in kuwait. | candida glabrata has emerged as an important human pathogen associated with systemic and mucosal infections. here, we describe isolation of two cholesterol-dependent candida glabrata strains from a candidemia patient which failed to grow on the media devoid of a cholesterol source. | 2014 | 24712485 |
| antioxidant and antifungal activities of smilax campestris griseb. (smilacaceae). | ethanol extract and fractions obtained from aerial parts of smilax campestris were examined in order to determine their phenolic composition, antioxidant capacity and antifungal activities. high-performance liquid chromatography coupled with dad analysis indicated that quercetin and rutin were the main phenolic compounds present in butanol fraction and ethanol extract, respectively. the antioxidant activity assessed by the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radical was significa ... | 2014 | 24707849 |
| enzymatic and hemolytic activity in different candida species. | candida species, in conditions of microbiota imbalance or decreased immune defenses, may be one of the main human fungal pathogens. virulence factors constitute the mechanisms used by the fungus to avoid host defenses. | 2015 | 24704439 |
| fatal case of candidemia due to candida glabrata. | 2014 | 24696185 | |
| candida glabrata and pre-term premature rupture of membrane complicating in vitro pregnancy: case report and confirmation of mother to neonate transmission. | we report a case of candida glabrata infection in an in vitro fertilization-assisted pregnancy complicated by pre-term pre-labor rupture of the membranes. we found c. glabrata in gastric fluid, amniotic fluid and maternal vaginal swab. neonatal and maternal isolates showed indistinguishable molecular patterns analyzed by fingerprint dna multilocus. | 2014 | 24691825 |
| severe systemic candidiasis following immunomodulation therapy in in vitro fertilisation-embryo transfer (ivf-et). | a 41-year-old nulliparous woman, with a medical history of unexplained infertility and multiple in vitro fertilisation (ivf) cycles with immunosuppressive therapy, was admitted to our tertiary obstetrics unit with sepsis at 18 weeks of pregnancy with dichorionic diamniotic twins. candida glabrata was grown from her blood cultures, then subsequently from the liquor and placentae. she was treated with intravenous ambisome (amphotericin), but unfortunately, the infection resulted in the rupture of ... | 2014 | 24686802 |
| in vitro evaluation of adherence of candida albicans, candida glabrata, and streptococcus mutans to an acrylic resin modified by experimental coatings. | this study evaluated the effect of experimental coatings, containing zwitterion or hydrophilic monomers, on the adherence of candida albicans, candida glabrata, and streptococcus mutans to an acrylic resin. acrylic samples (smooth or rough surfaces) were left untreated (control) or coated with one of the following experimental coatings: 3-hydroxypropylmethacrylate (hp) or sulfobetaine methacrylate (s), at concentrations of 25, 30, or 35%. half of the specimens were coated with saliva. the adhesi ... | 2014 | 24684564 |
| antifungal susceptibility of candida isolates at one institution. | species distribution and antifungal susceptibility of candida isolates at one institution were evaluated. detection rates of fungi were examined for 5 years between 2007 and 2011. sensitivities of fungi to amphotericin b, flucytosine, fluconazole, micafungin, itraconazole, and voriconazole were evaluated in blood culture-positive patients. a total of 3,832 fungal isolates were detected, including candida albicans 66.5%, candida glabrata 20.3%, candida parapsilosis 6.2%, candida tropicalis 5.5%, ... | 2014 | 24682093 |
| synthesis, structure-activity relationships, and in vitro antibacterial and antifungal activity evaluations of novel pyrazole carboxylic and dicarboxylic acid derivatives. | a series of pyrazole-3-carboxylic acid and pyrazole-3,4-dicarboxylic acid derivatives were synthesized, the structures were confirmed by their nmr ((1)h and (13)c) and ft-ir spectra, and elemental analyses. the antibacterial and antifungal activities of the compounds against five bacterial and five fungal pathogens were screened using modified agar well diffusion assay. most of the molecules have inhibitory effects on both standard and clinical candida albicans strains. however, only the molecul ... | 2014 | 24681068 |
| a novel echinocandin mig0310 with anticandida activity from newly isolated fusarium sp. strain ms-r1. | to isolate and identify an anticandida compound from newly isolated fusarium strain ms-r1 and characterization of its activity against standard and clinical strains of candida. | 2014 | 24674450 |
| species distribution and antifungal susceptibility profile of candida isolates from bloodstream infections in lima, peru. | yeast identification and in vitro susceptibility testing provide helpful information for appropriate administration of antifungal treatments; however, few reports from the latin american region have been published. the aim of this study was to identify the species present in isolates from bloodstream infections diagnosed in nine hospitals in lima, peru and to determine their in vitro susceptibility to four antifungal drugs. we tested and identified 153 isolates collected between october 2009 and ... | 2014 | 24667770 |
| candidemia surveillance in iowa: emergence of echinocandin resistance. | we performed prospective surveillance for candidemia at 14 iowa hospitals in 2011-2012. a total of 163 episodes were analyzed. candida albicans (n = 69 [42%]) and candida glabrata (n = 58 [36%]) were the most common species. antifungal resistance was uncommon; 9% of c. glabrata were fluconazole resistant, and 5% (3 isolates) were intermediate or resistant to 1 or more of the echinocandins. molecular analyses of the fks1 and fks2 hotspots of the c. glabrata revealed no mutations except in 2 of th ... | 2014 | 24666704 |
| phenotypic analysis of a family of transcriptional regulators, the zinc cluster proteins, in the human fungal pathogen candida glabrata. | candida glabrata is the second most important human fungal pathogen. despite its formal name, c. glabrata is in fact more closely related to the nonpathogenic budding yeast saccharomyces cerevisiae. however, less is known about the biology of this pathogen. zinc cluster proteins form a large family of transcriptional regulators involved in the regulation of numerous processes such as the control of the metabolism of sugars, amino acids, fatty acids, as well as drug resistance. the c. glabrata ge ... | 2014 | 24657903 |
| the impact of new epidemiological cutoff values on candida glabrata resistance rates and concordance between testing methods. | interpretive criteria for candida susceptibility testing were recently revised with the establishment of species-specific epidemiological cutoff values (ecv). to assess the effect of modified cutoff values on candida glabrata resistance rates and agreement between testing methods, we tested the susceptibility of 598 clinical isolates to fluconazole, itraconazole, voriconazole, posaconazole, caspofungin, and amphotericin b using clsi m27-a3 and e-test methods. the caspofungin mics clustered above ... | 2014 | 24657169 |
| in vitro differentiation of murine hematopoietic progenitor cells toward the myeloid lineage occurs in response to staphylococcus aureus and yeast species. | we have studied the effect of inactivated microbial stimuli (candida albicans, candida glabrata, saccharomyces boulardii, and staphylococcus aureus) on the in vitro differentiation of lineage negative (lin(-)) hematopoietic progenitor mouse cells. purified lin(-) progenitors were co-cultured for 7 days with the stimuli, and cell differentiation was determined by flow cytometry analysis. all the stimuli assayed caused differentiation toward the myeloid lineage. s. boulardii and particularly c. gl ... | 2014 | 24650426 |
| determination of candida species nestled in denture fissures. | with the growing number of elderly individuals, candida is occasionally recognised as a fungal cause of aspiration pneumonia. in addition, there are numerous investigational reports on oral candidiasis. however, there are currently no reports on candida contamination of denture base materials. this study was conducted to investigate candida species in the oral cavity, denture parts and oral lesions of older/elderly subjects with oral candidiasis. the candida strains were isolated and the species ... | 2013 | 24648980 |
| effects of fluconazole on candida glabrata biofilms and its relationship with abc transporter gene expression. | candida glabrata has emerged as the second most prevalent fungal pathogen and its ability to form biofilms has been considered one of the most important virulence factors, since biofilms present a high tolerance to antifungal agents used in fungal infection treatment. the mechanisms of biofilm tolerance to antifungal agents remain poorly understood. thus, the aim of this study was to evaluate the effects of fluconazole (flu) on the formation and control of c. glabrata biofilms and its relation w ... | 2014 | 24645630 |
| dual antifungal properties of cationic antimicrobial peptides polybia-mpi: membrane integrity disruption and inhibition of biofilm formation. | with the increasing emergence of resistant fungi, the discovery and development of novel antifungal therapeutics were urgently needed. compared with conventional antibiotics, the limited propensity of amps to induce resistance in pathogens has attracted great interest. in the present study, the antifungal activity and its mechanism-of-action of polybia-mpi, a cationic peptide from the venom of social wasp polybia paulista was investigated. we demonstrated that polybia-mpi could potently inhibit ... | 2014 | 24642357 |
| studies on the possible aetiological role of different candida species in pathogenesis of dentine caries by monitoring the calcium release from tooth particles. | an in vitro model has been developed for study of cariogenic potential of different candida species. slices were prepared from the root of extracted healthy teeth. these disks were covered with inert material, only the central hole, i.e. the root canal dentin surface remained uncovered. these preparates with free root dentin surfaces were incubated in sabouraud medium in the presence of six-six candida albicans, candida glabrata, candida tropicalis, candida krusei, candida inconspicua and candid ... | 2014 | 24631750 |
| the development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high-resolution melting curve analysis. | accurate, rapid and economical fungal species identification has been a major aim in mycology. in this study, our goal was to examine the feasibility of a high-resolution melting curve analysis (hrma) of internal transcribed regions its1 and its2 in ribosomal dna (rdna) for a rapid, simple and inexpensive differentiation of eight clinically relevant candida species (candida albicans, candida glabrata, candida parapsilosis, candida krusei, candida tropicalis, candida guilliermondii, candida dubli ... | 2014 | 24628973 |
| promising results of cranberry in the prevention of oral candida biofilms. | in the context of dental caries prevention by natural foodstuff sources, antifungal and antibiofilm activities of dry commercial extracts of cranberry fruit (vaccinium macrocarpon aiton) and two other red fruits (vaccinium myrtillus l. and malpighia punicifolia l.) were assessed on candida albicans and candida glabrata yeasts. when added to the culture medium, the cranberry extract displayed a significant anti-adhesion activity against candida spp. when used at low concentrations. in addition, t ... | 2014 | 24623607 |
| management of recurrent postoperative fungal endophthalmitis. | to report the management of recurrent postoperative fungal endophthalmitis (pofe) after failed pars plana vitrectomy (ppv) and antifungal therapy. | 2014 | 24618484 |
| metabolic engineering of candida glabrata for diacetyl production. | in this study, candida glabrata, an efficient pyruvate-producing strain, was metabolically engineered for the production of the food ingredient diacetyl. a diacetyl biosynthetic pathway was reconstructed based on genetic modifications and medium optimization. the former included (i) channeling carbon flux into the diacetyl biosynthetic pathway by amplification of acetolactate synthase, (ii) elimination of the branched pathway of α-acetolactate by deleting the ilv5 gene, and (iii) restriction of ... | 2014 | 24614328 |
| purification of g1 daughter cells from different saccharomycetes species through an optimized centrifugal elutriation procedure. | centrifugal elutriation discriminates cells according to their sedimentation coefficients, generating homogeneous samples well suited for genomic comparative approaches. it can, for instance, isolate g1 daughter cells from a saccharomyces cerevisiae unsynchronized population, alleviating ageing and cell-cycle biases when conducting genome-wide/single-cell studies. the present report describes a straightforward and robust procedure to determine whether a cell population of virtually any yeast spe ... | 2014 | 24604765 |
| isolation of antifungal compound from paeonia suffruticosa and its antifungal mechanism. | to isolate antifungal compound from paeonia suffruticosa, and to find the antifungal mechanisms by observing the ultrastructural modifications of yeasts in growth phase produced by 1,2,3,4,6-penta-o-galloyl-beta-d-glucose (pgg). | 2015 | 24577809 |
| candida glabrata drug:h+ antiporter cgtpo3 (orf cagl0i10384g): role in azole drug resistance and polyamine homeostasis. | the ability of opportunistic pathogenic candida species to persist and invade specific niches in the human host depends on their resistance to natural growth inhibitors and antifungal therapy. this work describes the role of the candida glabrata drug:h(+) antiporter cgtpo3 (orf cagl0i10384g) in this context. | 2014 | 24576949 |
| candida glabrata vertebral osteomyelitis in an immunosuppressed patient. | 2014 | 24576284 | |
| antifungal activity of phenolic compounds identified in flowers from north eastern portugal against candida species. | to evaluate the antifungal effect of gallic acid, catechin, luteolin and quercetin, phenolic compounds identified from flowers of north eastern portugal, against candida planktonic and biofilm cells. | 2014 | 24571069 |
| propargyl-linked antifolates are dual inhibitors of candida albicans and candida glabrata. | species of candida, primarily c. albicans and with increasing prevalence, c. glabrata, are responsible for the majority of fungal bloodstream infections that cause morbidity, especially among immune compromised patients. while the development of new antifungal agents that target the essential enzyme, dihydrofolate reductase (dhfr), in both candida species would be ideal, previous attempts have resulted in antifolates that exhibit inconsistencies between enzyme inhibition and antifungal propertie ... | 2014 | 24568657 |
| two non-exclusive strategies employed to protect torulopsis glabrata against hyperosmotic stress. | several recent reports described an apoptosis-like programmed cell death (pcd) process in yeast in response to different environmental challenges. in this study, hyperosmotic stress caused by high nacl concentration in culture medium induced cell death in the haploid yeast torulopsis glabrata. propidium iodide (pi) and pi/rhodamine-123 (rh123) dual staining with flow cytometry showed that high salinity decreased intact cells by 16.5 %, increased necrotic cells by nearly twofold, and altered ferm ... | 2014 | 24562390 |
| impact of therapeutic strategies on the prognosis of candidemia in the icu. | to determine the epidemiology of candida bloodstream infections, variables influencing mortality, and antifungal resistance rates in icus in spain. | 2014 | 24557426 |
| genome structure and dynamics of the yeast pathogen candida glabrata. | the yeast pathogen candida glabrata is the second most frequent cause of candida infections. however, from the phylogenetic point of view, c. glabrata is much closer to saccharomyces cerevisiae than to candida albicans. apparently, this yeast has relatively recently changed its life style and become a successful opportunistic pathogen. recently, several c. glabrata sister species, among them clinical and environmental isolates, have had their genomes characterized. also, hundreds of c. glabrata ... | 2014 | 24528571 |
| proteomic analysis of intracellular and membrane proteins from voriconazole-resistant candida glabrata. | the proteomic analysis of voriconazole resistant candida glabrata strain has not yet been investigated. in this study, differentially expressed proteins of intracellular and membrane fraction from voriconazole-susceptible, susceptible dose-dependent (s-dd), resistant c. glabrata strains were compared with each other and several proteins were identified. | 2013 | 24524017 |
| assessment of caspofungin susceptibility of candida glabrata by the etest®, clsi, and eucast methods, and detection of fks1 and fks2 mutations. | candida glabrata has emerged as a major pathogen in invasive candidiasis in recent years. currently, guidelines for invasive candidiasis treatment recommend fluconazole or an echinocandin as the first-line therapy. nevertheless, the resistance of candida glabrata to echinocandin is an emerging problem and has been partly associated with mutations in the fks1 and fks2 genes. the etest® is an appropriate method for determining antifungal susceptibility in emergency routine diagnosis. in this work, ... | 2014 | 24522619 |
| candida albicans isolates from a malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans candida isolates. | this study was aimed at determining the phospholipase and haemolysin activity of candida isolates in malaysia. a total of 37 candida clinical isolates representing seven species, candida albicans (12), candida tropicalis (8), candida glabrata (4), candida parapsilosis (1), candida krusei (4), candida orthopsilosis (1) and candida rugosa (7) were tested. in vitro phospholipase activity was determined by using egg yolk plate assay whereas in vitro haemolysin activity was tested by using blood plat ... | 2013 | 24522136 |
| micafungin pharmacokinetic/pharmacodynamic adequacy for the treatment of invasive candidiasis in critically ill patients on continuous venovenous haemofiltration. | to explore the pharmacokinetics (pk) and pharmacodynamics (pd) of micafungin in patients undergoing continuous venovenous haemofiltration (cvvh). | 2014 | 24505092 |
| formulation and in vitro evaluation of coconut oil-core cationic nanocapsules intended for vaginal delivery of clotrimazole. | the objective of this work was to propose coconut oil-core nanocapsules prepared from eudragit(®) rs100, a cationic polymer, and to evaluate their potential for vaginal delivery of clotrimazole in candidiasis. nanocapsule suspensions loaded with clotrimazole at 1.0 and 3.0mg/ml were prepared by interfacial deposition of eudragit(®) rs100. the physicochemical characterization showed average diameter lower than 200 nm, low polydispersity index, positive zeta potential (+10.94 to +14.57 mv), acid p ... | 2014 | 24503350 |
| recurrent catheter related bloodstream infections by candida glabrata: successful treatment with taurolidine. | 2014 | 24485770 | |
| [bilateral endogenous ophthalmitis due to candida glabrata after complicated bariatric surgery]. | a 43 year-old female presented with decreased visual acuity in the right eye. «snowball-like» retinal lesions were found in both eyes on examination. due to a lack of improvement with intravitreal antifungal empirical treatment, vitreous culture was performed and candida glabrata was isolated. the patient then received intravitreal amphotericin b, as well as systemic treatment with caspofungin and amphotericin b lipid complex. | 2014 | 24485524 |
| breakthrough candidemia due to multidrug-resistant candida glabrata during prophylaxis with a low dose of micafungin. | we identified a case of breakthrough candidemia in a 25-year-old patient receiving micafungin prophylaxis (50 mg/day). five candida glabrata isolates were obtained from blood cultures and were classified as multidrug-resistant isolates, since all of them exhibited high mics for echinocandin and azole drugs. a mutation (s663f) in hot spot 1 of the fks2 gene was found in all five isolates. this mutation yielded a 1,3-β-d-glucan synthase enzyme with highly reduced sensitivities to echinocandin drug ... | 2014 | 24468776 |
| in vitro effect of amphotericin b on candida albicans, candida glabrata and candida parapsilosis biofilm formation. | candida spp. biofilm is considered highly resistant to conventional antifungals. the aim of this study was to investigate the in vitro effect of amphotericin b on candida spp. biofilms at different stages of maturation. we investigated the activity of amphotericin b against 78 clinical isolates of candida spp., representing three species, growing as planktonic and sessile cells, by a widely accepted broth microdilution method. the in vitro effect on sessile cell viability was evaluated by mtt re ... | 2014 | 24436013 |
| the influence of chemical composition of commercial lemon essential oils on the growth of candida strains. | candida yeasts are saprophytes naturally present in the environment and forming colonies on human mucous membranes and skin. they are opportunistic fungi that cause severe and even fatal infections in immunocompromised individuals. several essential oils, including eucalyptus, pine, cinnamon and lemon, have been shown to be effective against candida strains. this study addresses the chemical composition of some commercial lemon essential oils and their antifungal potential against selected candi ... | 2014 | 24436010 |
| identifications of household's spores using mid infrared spectroscopy. | exposure to household fungi is very common both inside and outside the house and can cause health issues. the application of fourier transforms mid infrared spectroscopy (ftir) as a screening technique for the detection and identification of household fungi was investigated. early detection and identification of these household pathogens is very important and critical for their control. the current available methods for identification of fungi are time consuming, expensive and not very specific. ... | 2014 | 24434199 |
| candida nivariensis isolated from a renal transplant patient with persistent candiduria-molecular identification using its pcr and maldi-tof. | we report on the isolation of candida nivariensis from a renal transplant patient with persistent candiduria. biochemical profiling misidentified isolates as candida glabrata (3/5) and candida inconspicua (2/5). all isolates produced white colonies on chromagar(™) candida medium. internal transcribed spacer (its) ribosomal gene sequence analysis and maldi-tof-ms analysis (bruker biotyper(™) 2.0) identified all isolates as c. nivariensis, demonstrating the utility of maldi-tof as a rapid, accurat ... | 2013 | 24432244 |
| ten-year review of candidemia in a canadian tertiary care centre: predominance of non-albicans candida species. | to review the epidemiology and associated risk factors for candidemia at a tertiary care centre, in view of recent reports on the changing epidemiology of bloodstream infection due to candida species. | 2013 | 24421833 |
| impact of new antifungal breakpoints on antifungal resistance in candida species. | we reviewed our antifungal susceptibility data for micafungin, anidulafungin, fluconazole, and voriconazole against candida species and compared resistance rates determined by the previous and recently revised clsi antifungal breakpoints. with the new breakpoints, resistance was significantly increased for micafungin (from 0.8% to 7.6%), anidulafungin (from 0.9% to 7.3%), and voriconazole (from 6.1% to 18.4%) against candida glabrata. resistance was also increased for fluconazole against candida ... | 2014 | 24403302 |
| candida glabrata infection following total hip arthroplasty: a case report. | candida glabrata infection following total hip arthroplasty is rare and, due to the insufficiency of standardized clinical and evidence-based guidelines, there is no appropriate therapeutic schedule. the present study reports the case of a 44-year-old patient with candida glabrata infection following a total hip arthroplasty. the patient was successfully treated by administration of intravenous and oral voriconazole without removal of the prosthesis. this case illustrates the significance of pos ... | 2014 | 24396403 |
| a simple and inexpensive device for biofilm analysis. | the calgary biofilm device (cbd) has been described as a technology for the rapid and reproducible assay of biofilm susceptibilities to antibiotics. in this study a simple and inexpensive alternative to the cbd was developed from polypropylene (pp) microcentrifuge tubes and pipette tip boxes. the utility of the device was demonstrated using candida glabrata, a yeast that can develop antimicrobial-resistant biofilm communities. biofilms of c. glabrata were formed on the outside surface of microce ... | 2014 | 24389040 |
| establishment of an in vitro system to study intracellular behavior of candida glabrata in human thp-1 macrophages. | a cell culture model system, if a close mimic of host environmental conditions, can serve as an inexpensive, reproducible and easily manipulatable alternative to animal model systems for the study of a specific step of microbial pathogen infection. a human monocytic cell line thp-1 which, upon phorbol ester treatment, is differentiated into macrophages, has previously been used to study virulence strategies of many intracellular pathogens including mycobacterium tuberculosis. here, we discuss a ... | 2013 | 24378622 |
| update on antifungal resistance in aspergillus and candida. | antifungal resistance in candida and aspergillus may be either intrinsic or acquired and may be encountered in the antifungal drug exposed but also the antifungal drug-naïve patient. prior antifungal treatment confers a selection pressure and notoriously raises the awareness of possible resistance in patients failing therapy, thus calling for susceptibility testing. on the contrary, antifungal resistance in the drug-naïve patient is less expected and therefore more challenging. this is particula ... | 2014 | 24372701 |
| molecular identification and antifungal susceptibility of yeast isolates causing fungemia collected in a population-based study in spain in 2010 and 2011. | we report the molecular identifications and antifungal susceptibilities of the isolates causing fungemia collected in the candipop population-based study conducted in 29 spanish hospitals. a total of 781 isolates (from 767 patients, 14 of them having mixed fungemia) were collected. the species found most frequently were candida albicans (44.6%), candida parapsilosis (24.5%), candida glabrata (13.2%), candida tropicalis (7.6%), candida krusei (1.9%), candida guilliermondii (1.7%), and candida lus ... | 2014 | 24366741 |
| engineering of carboligase activity reaction in candida glabrata for acetoin production. | utilization of candida glabrata overproducing pyruvate is a promising strategy for high-level acetoin production. based on the known regulatory and metabolic information, acetaldehyde and thiamine were fed to identify the key nodes of carboligase activity reaction (car) pathway and provide a direction for engineering c. glabrata. accordingly, alcohol dehydrogenase, acetaldehyde dehydrogenase, pyruvate decarboxylase, and butanediol dehydrogenase were selected to be manipulated for strengthening t ... | 2014 | 24365210 |
| immune evasion, stress resistance, and efficient nutrient acquisition are crucial for intracellular survival of candida glabrata within macrophages. | candida glabrata is both a human fungal commensal and an opportunistic pathogen which can withstand activities of the immune system. for example, c. glabrata can survive phagocytosis and replicates within macrophages. however, the mechanisms underlying intracellular survival remain unclear. in this work, we used a functional genomic approach to identify c. glabrata determinants necessary for survival within human monocyte-derived macrophages by screening a set of 433 deletion mutants. we identif ... | 2014 | 24363366 |
| development of a luminex-based multiplex assay for detection of mutations conferring resistance to echinocandins in candida glabrata. | echinocandins are the recommended treatment for invasive candidiasis due to candida glabrata. resistance to echinocandins is known to be caused by nonsynonymous mutations in the hot spot-1 (hs1) regions of the fks1 and fks2 genes, which encode a subunit of the β-1,3-glucan synthase, the target of echinocandins. here, we describe the development of a microsphere-based assay using luminex magpix technology to identify mutations in the fks1 hs1 and fks2 hs1 domains, which confer in vitro echinocand ... | 2014 | 24353003 |
| role of dectin-2 for host defense against systemic infection with candida glabrata. | although candida glabrata is an important pathogenic candida species, relatively little is known about its innate immune recognition. here, we explore the potential role of dectin-2 for host defense against c. glabrata. dectin-2-deficient (dectin-2(-/-)) mice were found to be more susceptible to c. glabrata infections, showing a defective fungal clearance in kidneys but not in the liver. the increased susceptibility to infection was accompanied by lower production of t helper 1 (th1) and th17-de ... | 2014 | 24343653 |
| gpi (glycosylphosphatidylinositol)-linked aspartyl proteases regulate vacuole homoeostasis in candida glabrata. | a family of 11 gpi (glycosylphosphatidylinositol)-linked cell surface-associated aspartyl proteases (yapsins) in the human opportunistic fungal pathogen candida glabrata is required for cell wall remodelling, ph homoeostasis, survival in macrophages and virulence in a murine model of disseminated candidiasis. in the present paper, we report new roles for yapsins in c. glabrata physiology and implicate them for the first time in the regulation of vacuole homoeostasis. in the present study we show ... | 2014 | 24341558 |
| er stress response mechanisms in the pathogenic yeast candida glabrata and their roles in virulence. | the maintenance of endoplasmic reticulum (er) homeostasis is critical for numerous aspects of cell physiology. eukaryotic cells respond to the accumulation of misfolded proteins in the er (er stress) by activating the unfolded protein response (upr), an intracellular signaling pathway that adjusts the folding capacity of the er. recent studies of several pathogenic fungi have revealed that the upr is important for antifungal resistance and virulence; therefore, the pathway has attracted much att ... | 2014 | 24335436 |
| sorbic acid stress activates the candida glabrata high osmolarity glycerol map kinase pathway. | weak organic acids such as sorbic acid are important food preservatives and powerful fungistatic agents. these compounds accumulate in the cytosol and disturb the cellular ph and energy homeostasis. candida glabrata is in many aspects similar to saccharomyces cerevisiae. however, with regard to confrontation to sorbic acid, two of the principal response pathways behave differently in c. glabrata. in yeast, sorbic acid stress causes activation of many genes via the transcription factors msn2 and ... | 2013 | 24324463 |
| treatment of fungal disease in the setting of neutropenia. | invasive fungal infections are important causes of morbidity and attributable mortality in neutropenic patients with hematological malignancies, myelodysplasia, and aplastic anemia. successful risk-based strategies can be implemented for prophylaxis, empirical therapy, and preemptive therapy for the prevention and early treatment of invasive fungal infections in neutropenic hosts. the use of echinocandins for invasive candidiasis and voriconazole for invasive aspergillosis has significantly impr ... | 2013 | 24319214 |
| antimicrobial activity of extracts from in vivo and in vitro propagated lamium album l. plants. | the antimicrobial activity of 18 different extracts from in vivo and in vitro grown l. album l. plants was evaluated against clinical bacteria and yeasts using the well diffusion method. all the used extracts demonstrated antibacterial activity, whereas only the water extracts from leaves (in vivo) possessed antifungal activity against candida albicans nbimcc 72 and candida glabrata nbimcc 8673 (14 and 20 mm diameter of inhibition zones and mic 10 mg/ml, respectively). the methanol and ethanol e ... | 2013 | 24311888 |
| antimicrobial activities of some euphorbia species. | in this study, the antimicrobial activities of methanolic extracts and latex of some euphorbia species used for medical purposes in turkey were investigated. the extracts of euphorbia aleppica l., euphorbia szovitsii fisch.&mey. var. harputensis aznav. ex m. s. khan, euphorbia falcata l. sub. falcata var. falcata, euphorbia denticulata lam., euphorbia macroclada boiss., euphorbia cheiradenia boiss.&hohen, euphorbia virgata waldst.&kit., euphorbia petiolata banks&sol. were prepared with methanol. ... | 2013 | 24311840 |
| cell density impacts on candida glabrata survival in hypo-osmotic stress. | candida glabrata cells suspended in water are under hypo-osmotic stress and undergo cell death in 1-2 days, unless they are at a density of more than 10(5 ) cfu ml(-1) . the dying cells exhibit fitc-annexin v staining, indicative of programmed cell death (apoptosis). in a higher cell density, cells are protected and survive at least for 4 days. filtrates from cells at high density can protect those at lower density, indicating that cells release substances, amounting to c. 5 mg l(-1) of cell sus ... | 2014 | 24305439 |
| antifungal susceptibility and growth inhibitory response of oral candida species to brucea javanica linn. extract. | candida species have been associated with the emergence of resistant strains towards selected antifungal agents. plant products have been used traditionally as alternative medicine to ease candidal infections. the present study was undertaken to investigate the antifungal susceptibility patterns and growth inhibiting effect of brucea javanica seeds extract against candida species. | 2013 | 24305010 |
| repurposing as a means to increase the activity of amphotericin b and caspofungin against candida albicans biofilms. | biofilms of candida species, often formed on medical devices, are generally resistant to currently available antifungal drugs. the aim of this study was to identify compounds that increase the activity of amphotericin b and caspofungin, commonly used antifungal agents, against candida biofilms. | 2014 | 24284780 |
| antifungal application of nonantifungal drugs. | candida species are the cause of 60% of all mycoses in immunosuppressed individuals, leading to ∼150,000 deaths annually due to systemic infections, whereas the current antifungal therapies either have toxic side effects or are insufficiently efficient. we performed a screening of two compound libraries, the enzo and the institute for molecular medicine finland (fimm) oncology collection library, for anti-candida activity based on the european committee on antimicrobial susceptibility testing (e ... | 2014 | 24277040 |
| interactions between copy number and expression level of genes involved in fluconazole resistance in candida glabrata. | this study aimed to elucidate the relative involvement of drug resistance gene copy number and overexpression in fluconazole resistance in clinical c. glabrata isolates using a population-based approach. | 2013 | 24273749 |
| epidemiology of candidaemia and invasive candidiasis. a changing face. | invasive candidiasis is a leading cause of mortality. candidaemia is the most common clinical presentation of invasive candidiasis but more that 30% of these infections do not yield positive blood cultures. candida albicans remains the predominant aetiology, accounting for 50% of all cases. however, there has been an epidemiological shift in the last decades. some species of candida different to c. albicans have emerged as an important cause of severe candidaemia as they can exhibit resistance t ... | 2014 | 24270071 |
| the oxidative stress response of the opportunistic fungal pathogen candida glabrata. | organisms have evolved different strategies to respond to oxidative stress generated as a by-product of aerobic respiration and thus maintain the redox homeostasis within the cell. in particular, fungal pathogens are exposed to reactive oxygen species (ros) when they interact with the phagocytic cells of the host which are the first line of defense against fungal infections. these pathogens have co-opted the enzymatic (catalases, superoxide dismutases (sods), and peroxidases) and non-enzymatic ( ... | 2014 | 24270068 |
| biological activities of commercial bee pollens: antimicrobial, antimutagenic, antioxidant and anti-inflammatory. | bee pollen is considered, since memorable times, a good source of nourishing substances and energy. the present study aimed to evaluate the biological activities of eight commercial bee pollens purchased from the market. the origin of sample a was not specified in the labeling; samples b, c, d and g were from portugal and the remaining were from spain. the sample e presented the highest value of phenolics (32.15±2.12 mg/g) and the h the lowest (18.55±095 mg/g). sample c had the highest value of ... | 2014 | 24262487 |
| the mating type-like loci of candida glabrata. | candida glabrata, a haploid and opportunistic fungal pathogen that has not known sexual cycle, has conserved the majority of the genes required for mating and cell type identity. the c. glabrata genome contains three mating-type-like loci called mtl1, mtl2 and mtl3. the three loci encode putative transcription factors, a1, α1 and α2 that regulate cell type identity and sexual reproduction in other fungi like the closely related saccharomyces cerevisiae. mtl1 can contain either a or α information ... | 2014 | 24252826 |
| candida glabrata: a review of its features and resistance. | candida species belong to the normal microbiota of the oral cavity and gastrointestinal and vaginal tracts, and are responsible for several clinical manifestations, from mucocutaneous overgrowth to bloodstream infections. once believed to be non-pathogenic, candida glabrata was rapidly blamable for many human diseases. year after year, these pathological circumstances are more recurrent and problematic to treat, especially when patients reveal any level of immunosuppression. these difficulties a ... | 2014 | 24249283 |
| histatin 5-spermidine conjugates have enhanced fungicidal activity and efficacy as a topical therapeutic for oral candidiasis. | oropharyngeal candidiasis (opc) is caused by the opportunistic fungi candida albicans and is prevalent in immunocompromised patients, individuals with dry mouth, or patients with prolonged antibiotic therapies that reduce oral commensal bacteria. human salivary histatins, including histatin 5 (hst 5), are small cationic proteins that are the major source of fungicidal activity of saliva. however, hsts are rapidly degraded in vivo, limiting their usefulness as therapeutic agents despite their lac ... | 2014 | 24247141 |
| chemical composition and antimicrobial activity of the essential oil from the edible aromatic plant aristolochia delavayi. | the essential oil obtained by hydrodistillation from the aerial parts of aristolochia delavayi franch. (aristolochiaceae), a unique edible aromatic plant consumed by the nakhi (naxi) people in yunnan, china, was investigated using gc/ms analysis. in total, 95 components, representing more than 95% of the oil composition, were identified, and the main constituents found were (e)-dec-2-enal (52.0%), (e)-dodec-2-enal (6.8%), dodecanal (3.35%), heptanal (2.88%), and decanal (2.63%). the essential oi ... | 2013 | 24243612 |
| mapping of mitochondrial dna from torulopsis glabrata : location of ribosomal and transfer rna genes. | large and small rrnas have been isolated from mitochondria of the yeast torulopsis glabrata and have been shown to have lengths of 2,700 bases and 1,400 bases respectively. construction of a restriction endonuclease site map of mitochondria) dna has enabled us to position these rrnas by hybridization of labelled rna to dna fragments transferred to nitrocellulose. the large and small mt rrna genes are separated by a minimum of 1,820 by and a maximum of 2,765 by on the 18,870 by mitochondria1 geno ... | 1980 | 24189661 |
| segregation and transmission of mitochondrial markers in fusion products of the asporogenous yeast torulopsis glabrata. | using a protoplast fusion technique we have been able to locate to the mitochondrial genome of the asporogenous yeast torulopsis glabrata mutations conferring resistance to oligomycin, antimycin and diuron. when two strains differing in the size of their mtdnas were fused the mitochondrial markers from the parent with the larger mtdna (71-91) were transmitted predominantly among the fusion products. both genetical and physical evidence support the occurrence of recombination in t. glabrata mitoc ... | 1981 | 24185871 |
| nosocomial bloodstream infections due to candida spp. in the usa: species distribution, clinical features and antifungal susceptibilities. | candida spp. are among the most frequent nosocomial pathogens, contributing significantly to morbidity and mortality. longitudinal data on the epidemiology of candida bloodstream infections (bsis) are still limited. isolates and clinical data from 1218 episodes of candida bsi were prospectively collected from patients in 52 hospitals in the usa between 1998 and 2006. susceptibilities to amphotericin b, flucytosine, fluconazole, posaconazole, voriconazole, anidulafungin, caspofungin and micafungi ... | 2014 | 24182454 |
| prevalence and risk factors of onychomycosis in primary school children living in rural and urban areas in central anatolia of turkey. | onychomycosis is a world-wide public health concern in children, requiring epidemiological data for different regions for control and prevention. | 2013 | 24177609 |
| possible chromosomal location for the killer determinant in torulopsis glabrata. | we have used cytoduction and protoplast fusion to characterise the killer trait in torulopsis glabrata. killer trait could not be transferred by cytoduction to a non-killer strain of t. glabrata suggesting that the determinant(s) is/are not present in the cytoplasm. consistent with this interpretation is the observation that all fusion products, where chromosomes of both the parents are represented, are killers. non-killer segregants could be isolated from fusion products after treatment with be ... | 1984 | 24177585 |
| the yeast anaerobic response element ar1b regulates aerobic antifungal drug-dependent sterol gene expression. | saccharomyces cerevisiae ergosterol biosynthesis, like cholesterol biosynthesis in mammals, is regulated at the transcriptional level by a sterol feedback mechanism. yeast studies defined a 7-bp consensus sterol-response element (sre) common to genes involved in sterol biosynthesis and two transcription factors, upc2 and ecm22, which direct transcription of sterol biosynthetic genes. the 7-bp consensus sre is identical to the anaerobic response element, ar1c. data indicate that upc2 and ecm22 fu ... | 2013 | 24163365 |
| proteomic analysis of cellular and membrane proteins in fluconazole-resistant candida glabrata. | candida glabrata is one of the most common causes of candida bloodstream infections worldwide. some isolates of c glabrata may be intermediately resistant to azoles, with some strains developing resistance during therapy or prophylaxis with fluconazole. in this study, we used a proteomic approach to identify differentially expressed proteins between fluconazoleresistant and -susceptible strains. | 2012 | 24159494 |
| gene expression and identification related to fluconazole resistance of candida glabrata strains. | candida glabrata has become one of the most common causes of candida bloodstream infections worldwide. some strains of c. glabrata may be intermediately resistant to all azoles. the several possible mechanisms of azole resistance have been reported previously, but the exact resistant mechanism is not clear. in this study, we identified differentially expressed genes (degs) of fluconazole-resistant c. glabrata and compared the gene expression of fluconazole-resistant strains with that of fluconaz ... | 2010 | 24159438 |
| a patient with interstitial lung disease secondary to dermatomyositis: a case report and review of the literature. | we describe the case of a man with known dermatomyositis who presented with a dry cough and who early after admission rapidly evolved to respiratory insufficiency. based on pathological and radiological findings, the diagnosis of diffuse alveolar damage was made. postmortem examination also revealed infection with yeast, torulopsis glabrata. polymyositis (pm) and dermatomyositis (dm) are both auto-immune diseases, which are characterised by the presence of auto-antibodies and tissue-inflammation ... | 2016 | 24156232 |
| use of micafungin as a surrogate marker to predict susceptibility and resistance to caspofungin among 3,764 clinical isolates of candida by use of clsi methods and interpretive criteria. | due to unacceptably high interlaboratory variation in caspofungin mic values, we evaluated the use of micafungin as a surrogate marker to predict the susceptibility of candida spp. to caspofungin using reference methods and species-specific interpretive criteria. the mic results for 3,764 strains of candida (eight species), including 73 strains with fks mutations, were used. caspofungin mic values and species-specific interpretive criteria were compared with those of micafungin to determine the ... | 2014 | 24153129 |
| characterization of the recombinant candida albicans β-1,2-mannosyltransferase that initiates the β-mannosylation of cell wall phosphopeptidomannan. | the presence of β-mannosides in their cell walls confers specific features on the pathogenic yeasts candida albicans and candida glabrata compared with non-pathogenic yeasts. in the present study, we investigated the enzymatic properties of bmt1 (β-mannosyltransferase 1), a member of the recently identified β-mannosyltransferase family, from c. albicans. a recombinant soluble enzyme lacking the n-terminal region was expressed as a secreted protein from the methylotrophic yeast pichia pastoris. i ... | 2014 | 24138199 |
| comparison of the t2dx instrument with t2candida assay and automated blood culture in the detection of candida species using seeded blood samples. | as a delay in the diagnosis and treatment of candidemia is associated with increased mortality and healthcare costs, a more rapid method of detection is urgently needed. the t2candida assay is a new rapid diagnostic test, which uses t2 magnetic resonance technology to identify candida spp. directly from whole blood in approximately 3 hours. in this study, the performance of the bactec 9050 using aerobic plus/f blood culture bottles was compared to that of the t2candida assay run on the t2dx inst ... | 2013 | 24135411 |
| structural studies and investigation on the activity of imidazole-derived thiosemicarbazones and hydrazones against crop-related fungi. | new imidazole derived thiosemicarbazones and hydrazones were prepared by condensation of 4(5)-imidazole carboxaldehyde, 4-(1h-imidazole-1-yl)benzaldehyde and 4-(1h-imidazole-1-yl)acetophenone with a thiosemicarbazide or hydrazide. all compounds were characterized by quantitative elemental analysis, ir and nmr techniques. eight structures were determined by single crystal x-ray diffraction. the antifungal activities of the compounds were evaluated. none of the compounds exhibited significant acti ... | 2013 | 24129274 |
| frequency of fks mutations among candida glabrata isolates from a 10-year global collection of bloodstream infection isolates. | among 119 echinocandin non-wild-type (non-wt) candida glabrata strains from two global surveys, mutations in fks hot spots (hss) were detected in 28 (from 7 countries and 8 u.s. states): 24 strains (85.7%) had non-wt mics for micafungin, 22 (78.6%) for anidulafungin, and 25 (89.3%) for caspofungin. the most common fks substitutions among non-wt strains were at positions f659 (n = 7) and s663 (n = 7). three isolates displaying wt mic results had f625y, l630i, and d632y substitutions or non-hs mut ... | 2014 | 24126582 |
| epidemiology and predictive factors for early and late mortality in candida bloodstream infections: a population-based surveillance in spain. | a prospective, multicentre, population-based surveillance programme for candida bloodstream infections was implemented in five metropolitan areas of spain to determine its incidence and the prevalence of antifungal resistance, and to identify predictors of death. between may 2010 and april 2011, candida isolates were centralized to a reference laboratory for species identification by dna sequencing and for susceptibility testing by eucast reference procedure. prognostic factors associated with e ... | 2014 | 24125548 |
| urea enhances cell growth and pyruvate production in torulopsis glabrata. | torulopsis glabrata is a strain of yeast that is used for the industrial production of pyruvate. determination of the optimal nutrient environment is vital for obtaining the most efficient production system. in this study, the fermentation parameters, gene transcription levels, activities of key enzymes and metabolites levels were analyzed when either urea or ammonium chloride was used as the sole source of nitrogen. urea caused an increase in the dry cell weight (18%) and pyruvate productivity ... | 2014 | 24124177 |
| changing epidemiology of candidemia in a medical center in middle taiwan. | candidemia remains a major cause of morbidity and mortality in the health care setting, and the epidemiology of candida infection is changing. | 2015 | 24113067 |
| epidemiology, species distribution, antifungal susceptibility, and outcome of candidemia across five sites in italy and spain. | candidemia has become an important bloodstream infection that is frequently associated with high rates of mortality and morbidity, and its growing incidence is related to complex medical and surgical procedures. we conducted a multicenter study in five tertiary care teaching hospitals in italy and spain and evaluated the epidemiology, species distribution, antifungal susceptibilities, and outcomes of candidemia episodes. in the period of 2008 to 2010, 995 episodes of candidemia were identified i ... | 2013 | 24108614 |