Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| intracellular damage in yeast cells caused by photodynamic treatment with toluidine blue. | the positively charged photosensitizer toluidine blue (tb) can induce loss of clonogenicity in kluyveromyces marxianus. previous studies have revealed that, as a consequence of the localization of this dye at the cell surface, photodynamic action results in extensive damage at the level of the plasma membrane. in this paper, a study is reported on the effect of photodynamic treatment with tb on intracellular enzymes. it is shown that treatment with tb and light resulted in the inhibition of alco ... | 1995 | 7899497 |
| structure-activity relationships and interactions among trichothecene mycotoxins as assessed by yeast bioassay. | a yeast (kluyveromyces marxianus) bioassay was used to establish the relative toxicity of 16 trichothecenes and some of their interactions. removal of an isovaleryl group, one or two acetyl groups, or two acetyl plus an isovaleryl groups from t-2 toxin (t2) to form diacetoxyscirpenol, ht-2 toxin (ht2), t2-triol and t-2 tetraol (t2-4ol) decreased toxicity 7, 36, 276 and 558-fold, respectively. a combination of t2 and ht2, t2 and t2-4ol, deoxynivalenol (don) and nivalenol (niv), or don and t2 exhi ... | 1994 | 7801350 |
| the influence of pressure and temperature of compressed co2 on the survival of yeast cells. | in order to study the potential use of supercritical co2 extraction for the recovery of products from yeast cell cultures, the effects of co2 on different yeast strains over a range of pressures and temperatures have been examined. viability was shown to be dependent on temperature and dissolved co2 concentration, and can be described by a sigmoidal (s-shaped) curve. cell death was mainly due to an 'anaesthesia effect' rather than cell rupture. important differences in sensitivity were observed ... | 1995 | 7766403 |
| expression of an alpha-galactosidase gene under control of the homologous inulinase promoter in kluyveromyces marxianus. | for expression of the alpha-galactosidase gene from cyamopsis tetragonoloba in kluyveromyces marxianus cbs 6556 we have used the promoter of the homologous inulinase-encoding gene (inu1). the inu1 gene has been cloned and sequenced and the coding region shows an identity of 59% with the saccharomyces cerevisiae invertase gene (suc2). in the 5'-flanking region of inu1 we found a sequence (taaatccgggg) that perfectly matches to the mig1 binding consensus sequence (wwwwtsygggg) of the s. cerevisiae ... | 1993 | 7764385 |
| preparation and characterization of whey protein hydrolysates: applications in industrial whey bioconversion processes. | a whey protein hydrolysate was prepared by incubation of reconstituted whey or a whey protein concentrate with alcalase 0.6l. the proteolytic degradation of alpha-lactalbumin and beta-lactoglobulin initially resulted in 6-kda and, later, 2.5-kda degradation products, quickly followed by the appearance of multiple peptides of 1 kda or smaller. the hydrolysate showed a steady increase in solubility and a biphasic change in foaming characteristics with decreasing peptide size. at the highest degree ... | 1993 | 7763629 |
| two tryptophans at the active site of udp-glucose 4-epimerase from kluyveromyces fragilis. | efficient fluorescence energy transfer from aromatic residues to the pyridine moiety of the bound coenzyme (nad) of udp-glucose 4-epimerase from kluyveromyces fragilis had been reported earlier (mukherji, s., and bhaduri, a. (1992) j. biol. chem. 267, 11709-11713). we have employed n-bromosuccinimide (nbs) to identify tryptophan as the exclusive aromatic donor in the energy transfer. the characteristic uv absorption spectrum associated with trp oxidation is observed during nbs modification of tw ... | 1995 | 7744774 |
| comparison of high-performance liquid chromatography and bioassay for the determination of 5-fluorocytosine in serum. | an hplc method using a reverse phase system, an isocratic mobile phase and simple protein precipitation and a plate diffusion bioassay using an amphotericin b resistant strain of candida pseudotropicalis for the measurement of 5-fluorocytosine in serum were compared. both methods permit a determination of 5-fluorocytosine in sera also in the presence of amphothericin b. the correlation between bioassay and hplc runs was found to be r = 0.96. both methods are useful for monitoring the serum level ... | 1994 | 7727899 |
| kluyveromyces marxianus small dna fragments contain both autonomous replicative and centromeric elements that also function in kluyveromyces lactis. | two fragments containing both an autonomous replicating sequence (ars) and a centromere have been isolated and sequenced from the yeast kluyveromyces marxianus. the ars and centromeric core sequences are only 500 bp apart, but ars activity could be separated from the centromeric sequences. centromeric sequences are organized in a similar way to those of budding yeasts: two well-conserved elements: cdei (5' tcacgtg 3') and cdeiii (5' tnttccgaaagtwaaa 3'), are separated by a 165 bp at-rich (+/- 90 ... | 1994 | 7725797 |
| polymorphonuclear phagocytosis and killing in workers exposed to inorganic mercury. | the ability of neutrophils to phagocytose and kill candida species as well as the splenic phagocytic function were investigated in workers from a mercury-producing plant. in the neutrophil phagocytosis study, two species of candida were used since in individuals with myeloperoxidase deficiency neutrophils are unable to kill candida albicans, while candida pseudotropicalis can be effectively lysed. phagocytosis of both antigens and splenic phagocytic function were normal in all the workers studie ... | 1994 | 7705962 |
| adsorption of kluyveromyces marxianus pectinase on cm-sephadex gels. | new results are presented on the equilibria and kinetics of the ion exchange onto cm-sephadex of polygalacturonase (pectinase) produced by the fermentation of kluyveromyces marxianus. it is found that the equilibrium behavior follows the form of the langmuir isotherm; the equilibrium is strongly affected by ph. high partitioning onto the ion-exchange matrix, with good retention of enzyme activity, is achieved in the ph range 3.5-5.0, and this can be qualitatively explained in terms of simple mod ... | 1993 | 7694598 |
| the mig1 repressor from kluyveromyces lactis: cloning, sequencing and functional analysis in saccharomyces cerevisiae. | sequence comparisons between saccharomyces cerevisiae scmig1 and aspergillus nidulans crea proteins allowed us to design two sets of degenerate primers from the conserved zinc finger loops. pcr amplification on kluyveromyces marxianus and k. lactis genomic dna yielded single products with sequences closely related to each other and to the corresponding regions of scmig1 and crea. the kimig1 gene of k. lactis was cloned from a genomic library using the k. marxianus pcr fragment as probe. kimig1 e ... | 1995 | 7672126 |
| characterization of the glyceraldehyde-3-phosphate dehydrogenase gene family from kluyveromyces marxianus--polymerase chain reaction-single-strand conformation polymorphism as a tool for the study of multigenic families. | three glyceraldehyde-3-phosphate dehydrogenase (gapdh) genes from kluyveromyces marxianus were identified and characterized. the coding region of two of them (gap2 and gap3) is very similar (99.6% homology). the other gene (gap1) is only 86% homologous to gap2 or gap3 and is responsible for the expression of gap1p. this protein is extremely homologous to the k. marxianus cell wall protein p37, presumably involved in flocculation. however, no leader sequence could be detected in this gene. the id ... | 1995 | 7668042 |
| inducible expression of a gene encoding an l41 ribosomal protein responsible for the cycloheximide-resistant phenotype in the yeast candida maltosa. | in a previous paper (s. kawai, s. murao, m. mochizuki, i. shibuya, k. yano, and m. takagi, j. bacteriol. 174:254-262, 1992), we showed that in each genome of several yeast species, there is one of two types of l41 gene, one for an l41 (q-type) protein which confers cycloheximide (cyh) resistance or one for an l41 (p-type) protein which does not. these genes have been suggested to be responsible for the cyh response used in taxonomy. for example, saccharomyces cerevisiae, which is cyh sensitive, ... | 1995 | 7665534 |
| protective effect of amphotericin b against lethal photodynamic treatment in yeast. | the effect of polyenic antibiotic amphotericin b on photodynamically induced cell damage was investigated using kluyveromyces fragilis. the photosensitizers applied are known to act via cell membrane damage (rose bengal and toluidine blue) or via dna modification causing genotoxic effects (8-methoxypsoralen). methylene blue was shown to cause membrane damage comparable with the effect of rose bengal and toluidine blue. under conditions of photodynamic damage a pronounced protective effect of the ... | 1995 | 7630327 |
| synthesis and biological activities of some 3,6-disubstituted thiazolo[3,2-b][1,2,4]triazoles. | some new 2,3-dihydro-3-hydroxy-6-phenyl-3-(4-substituted- (phenylthiazolo[3,2-b][1,2,4]triazole derivatives were synthesized as antifungal agents. after their structures were confirmed by microanalysis and ir and nmr spectral analysis, their antifungal activities against candida albicans, candida parapsilosis, candida stellatoidea, and candida pseudotropicalis were investigated. contrary to our expectations, all proved to have poor antifungal activities. because 2,4-dihydro-3h-1,2,4-triazol-3-on ... | 1995 | 7629737 |
| effect of t-2 toxin and verrucarin a in combination on kluyveromyces marxianus. | the growth inhibitory effects of combinations of t-2 toxin and verrucarin a on the yeast kluyveromyces marxianus was studied. a combination index value was derived to indicate the type of interaction that existed between the binary mixture of these two toxins at various ratios and the target yeast cells. the type of interaction was dependent on the ratio of the toxins used to attain a particular level of growth inhibition. further, the least change in the type and intensity of interaction or the ... | 1995 | 7613734 |
| synthesis of 1,2,5(6)-trisubstituted benzimidazoles and evaluation of their antimicrobial activities. | a series of 22 benzimidazoles, having several substituents on the azole and benzene nuclei, were prepared and evaluated in vitro for antimicrobial activity. at first 2-chloro or 2-chloromethyl-5(6)-substituted-1h-benzimidazoles were synthesized, which were then substituted at c-2 with several piperazine or piperidine derivatives. the antibacterial activity of these compounds against staphylococcus aureus, bacillus subtilis, escherichia coli, and pseudomonas aeruginosa, and the antifungal activit ... | 1995 | 7611838 |
| selection of strain, culture conditions and extraction procedures for optimum production of beta-galactosidase from kluyveromyces fragilis. | among 47 tested yeast strains, belonging to different genera, only two cultures of kluyveromyces fragilis and fabospora fragilis showed beta-galactosidase activity in shaken flasks. three types of extraction were used to release the enzyme from k. fragilis cells: solvent and detergent extraction, freezing and thawing extraction, and mechanical disintegration prior to extraction, using triton x-100. the results indicate that the highest yield could be obtained by mechanical disintegration of cell ... | 1994 | 7526616 |
| inhibition of fungal growth by pseudomonas aeruginosa and pseudomonas cepacia isolated from patients with cystic fibrosis. | this study was undertaken because of the infrequency of infections due to candida species in patients with cystic fibrosis despite their extensive treatment with broad-spectrum antibiotics. in vitro susceptibility studies revealed significant inhibition of 11 strains of fungi known to infect human beings by 10 strains of pseudomonas aeruginosa and nine strains of pseudomonas cepacia isolated from the sputum of patients with cystic fibrosis. the fungi were candida krusei, candida keyfr, candida g ... | 1994 | 7522262 |
| studies on metal resistance system in kluyveromyces marxianus. | through preliminary plate tests, kluyveromyces marxianus was found to be much more resistant to toxic heavy metals compared to a cup1r strain of saccharomyces cerevisiae. specific growth rate and maximum dry weights affected by increasing metal concentrations were determined to obtain precise patterns of resistance. metal biosorption was also monitored during the course of growth in synthetic media containing respective metals at 0.5 mm final concentration. although zn- and co-binding was neglig ... | 1993 | 7508248 |
| identification of candida species by pcr and restriction fragment length polymorphism analysis of intergenic spacer regions of ribosomal dna. | the pcr was used to amplify a targeted region of the ribosomal dna from 84 candida isolates. unique product sizes were obtained for candida guilliermondii, candida (torulopsis) glabrata, and candida pseudotropicalis. isolates of candida albicans, candida tropicalis, candida stellatoidea, candida parapsilosis, and candida krusei could be identified following restriction digestion of the pcr products. | 1995 | 7494052 |
| the effects of human immunodeficiency virus infection on macrophage phagocytosis of candida. | the effects of infection by human immunodeficiency virus (hiv) on phagocytic function towards candida pseudotropicalis were tested using monocytotropic and nonmonocytotropic hiv strains on peripheral blood monocytes. there were no differences in either quantitative phagocytic activity or efficacy between the two infected and control mock-infected monocytes. immunofluorescence for cytoplasmic p24 antigen and in situ hybridization for detection of hiv-specific rna sequences revealed that very few ... | 1994 | 7478756 |
| ribosomal rna genes in kluyveromyces marxianus. | dna from the yeast kluyveromyces marxianus was studied for its heterogeneity and the multiplicity of rrna cistrons. these cistrons banded at a slightly different density from the bulk dna in preparative cscl or hg2+-cs2so4 equilibrium density gradients. the reassociation kinetics of the bulk dna showed that the repetitive fraction represented a small amount of the total cellular dna (10%) and that the single copy fraction had a complexity of 6.3 x 10(9) daltons. approximately 2.2% of the dna hyb ... | 1980 | 7420052 |
| the prevalence of yeasts in clinical specimens from cancer patients. | yeasts recovered from cancer patients during a 15-month period were speciated, and the prevalence of these isolates in various types of clinical specimens was determined. five species, including candida albicans, candida tropicalis, candida parapsilosis, candida krusei, and torulopsis glabrata, accounted for 97.1% of the isolates. eighteen different species were recovered. respiratory and urine specimens yielded 75% of the organisms. c. albicans, c. tropicalis, and c. parapsilosis were recovered ... | 1980 | 7369176 |
| catabolite repression-inactivation of malate dehydrogenase in candida kefyr. | 1981 | 7309169 | |
| selection of yeast strains for ethanol production from inulin. | of the many yeast species capable of fermenting inulin, some can produce sufficient amounts of ethanol from the substrate, in particular kluyveromyces fragilis and torulopsis colliculosa. the results indicate the feasibility of producing ethanol from inulin-rich plants, such as jerusalem artichoke. | 1981 | 7262714 |
| rate of arabinitol production by pathogenic yeast species. | d-arabinitol is a five-carbon polyol that is produced by many fungi. detection of the metabolite has been reported in serum from patients with invasive candidiasis. we studied the production and assimilation of arabinitol by 46 clinical isolates of yeast species. cultures of isolates of candida albicans (9 strains), candida tropicalis (12 strains), candida parapsilosis (13 strains), candida krusei (4 strains), candida pseudotropicalis (3 strains), torulopsis glabrata (3 strains), and cryptococcu ... | 1981 | 7024306 |
| kluyveromyces fragilis as an opportunistic fungal pathogen in man. | an immunosuppressed cardiac transplant patient with pulmonary infection due to the yeast kluyveromyces fragilis is described. isolation of this fungus from human sources is rarely reported, and previous reports of human infection are unavailable in the modern literature. the organism is poorly pathogenic even in immunocompromised hosts. in vitro susceptibility studies with several strains presented here indicate inhibition by 5-fluorocytosine and miconazole, and borderline susceptibility to amph ... | 1980 | 6988992 |
| effect of ph on the growth of kluyveromyces fragilis on deproteinized whey. | 1980 | 6894053 | |
| [production, extraction and partial characterization of beta-d-galactosidase of kluyveromyces fragilis grown in milk whey]. | 1980 | 6894052 | |
| purification and properties of the beta-fructofuranosidase from kluyveromyces fragilis. | the beta-fructofuranosidase from kluyveromyces fragilis was purified to one band on electrophoresis by 3 different methods. two of the preparations were found to be impure by isoelectric focusing. this demonstrates the need for more than one criteria of homogeneity when purifying this enzyme. the enzyme was found to be a glycoprotein, stable at 50 degrees c, with a ph optimum of 4.5. the cations hg2+, ag+, cu2+ and cd2+ exhibited a marked inhibition of the enzyme. competitive inhibition was obse ... | 1983 | 6884506 |
| amphotericin b tolerance: a characteristic of candida parapsilosis not shared by other candida species. | thirty yeast isolates from clinical specimens were tested for their susceptibility to amphotericin b at 30 c, 37 c, and 39 c. of the six candida albicans, five candida tropicalis, one candida guilliermondii, one candida krusei, one candida pseudotropicalis, two torulopsis glabrata, and four cryptococcus neoformans isolates tested, all were inhibited at amphotericin b concentrations of less than or equal to 0.4 micrograms/ml and killed by concentrations of amphotericin b that were less than or eq ... | 1983 | 6822746 |
| regulation of beta-d-galactosidase synthesis in candida pseudotropicalis. | regulation of lactose (beta-d-galactosidase) synthesis in the lactose-utilizing yeast candida pseudotropicalis was studied. the enzyme was inducible by lactose and galactose. when grown on these sugars the enzyme level of the yeast was 20 times or higher than when grown on glycerol. the km and optimal ph were similar for the lactase induced either by lactose or galactose. the hydrolysis of o-nitrophenyl-beta-d-galactopyranoside by the lactase was inhibited by galactose and several analogs and ga ... | 1982 | 6800304 |
| production of beta-galactosidase from kluyveromyces fragilis grown on whey. | optimum conditions for beta-galactosidase production by k. fragilis were studied. enzyme production has a maximum after 8-12 h of incubation. composition of whey (from different sources) did not affect enzyme production. different heart treatments also had no effect. whey reconstituted to 8-12% total solids and adjusted to ph 4.0 afforded maximum enzyme production. whereas inorganic nitrogen sources (specially ammonium salts) only slightly stimulated enzyme production, organic nitrogen sources ( ... | 1981 | 6797906 |
| impaired monocyte function in liver cirrhosis. | monocyte function in patients with cirrhosis of the liver was measured by phagocytosis and killing of candida pseudotropicalis and c albicans. both variables were significantly decreased in the patients compared with controls. control monocytes exposed for two hours to patients' serum showed a significant decrease in intracellular killing compared with control monocytes incubated in autologous serum. this suggests the presence of an inhibiting factor in the patients' serum. this inhibitory facto ... | 1981 | 6784806 |
| [phagocytosis and lysis of candida albicans and candida pseudotropicalis by polymorphonuclear neutrophils of hansen's disease patients]. | the phagocytosis and lysis of candida albicans and candida pseudotropicalis by human neutrophils were examined in 43 hanseniasis patients (19 quiescent virchowians, 12 reactional virchowians and 12 tuberculoid patients) and 15 healthy subjects. the purpose of this study was to determine whether neutrophils from hanseniasis patients were altered in their enzyme systems myeloperoxidase-dependent (tested by lysin of candida albicans) and myeloperoxidase-independent (tested by lysin of candida pseud ... | 1981 | 6759396 |
| alveolar macrophage dysfunction in human bone marrow transplant recipients. | we studied the functional characteristics of alveolar macrophages obtained by segmental pulmonary lavage from allogeneic marrow transplant recipients without evidence of ongoing pulmonary infection. the macrophages were mostly of donor marrow origin as judged by y body fluorescence and were morphologically normal, except for the intracellular accumulation of various amounts of heterogeneous foreign materials. macrophage function of patients studied within four months after transplantation was im ... | 1982 | 6756138 |
| candida pseudotropicalis fungemia and invasive disease in an immunocompromised patient. | a case of candida pseudotropicalis fungemia and invasive disease in an immunocompromised patient is reported. multiple blood cultures taken over a 2-week period were positive, and histopathological slides of postmortem spleen and kidney tissue showed tissue invasion by the organism. the source of the yeast infection was determined to be the urinary tract. this is the first report of c. pseudotropicalis fungemia documented by culture. | 1984 | 6511866 |
| development of hodgkin's disease in the course of liver cirrhosis and impaired monocyte function. | the monocyte function, as measured by phagocytosis and killing of candida albicans and candida pseudotropicalis by peripheral blood monocytes, was found impaired in a patient with cirrhosis of the liver on two separate determinations before the occurrence of bleeding from oesophageal varices. unexpectedly, hodgkin's disease was diagnosed in enlarged abdominal lymph nodes found on the occasion of an emergency portocaval shunt operation. | 1984 | 6463000 |
| the cell wall-associated inulinase of kluyveromyces fragilis. | the yeast kluyveromyces fragilis (atcc 12424) was grown on a 2% inulin-1% yeast extract medium for 36 h and subsequently fixed with 0.5% glutaraldehyde. the glutaraldehyde treatment did not affect the beta-fructofuranosidase (inulinase, ec 3.2.1.7) activity of the cells but it did make the cells resistant to chemical and physical treatments that normally release beta-fructofuranosidase from untreated cells. the enzyme in the treated cells exhibited km values for sucrose and raffinose identical t ... | 1984 | 6441514 |
| in vitro effect of mycobacterium leprae suspensions on the polymorphonuclear neutrophils function of hanseniasis patients to candida albicans and candida pseudotropicalis. | the in vitro effect of mycobacterium leprae suspensions on the pmn ability to phagocytizing and killing candida albicans and candida pseudotropicalis was studied in forty-five patients of hansen's disease and in fifteen healthy controls. our results show no significative differences between the different studied groups, both for the phagocytosis and for the lysis of yeasts. there was no significant changes in the mean values of these functions after previous or simultaneously incubation with myc ... | 1984 | 6400664 |
| survey of sensitivity of twelve yeast genera toward t-2 toxin. | a survey was made to detect the sensitivity of 12 yeast genera to t-2 toxin. seventy-five yeasts isolated from various sources were tested for their susceptibility to t-2 toxin. the mic of t-2 for these yeasts varied from 1.0 to greater than 8.0 micrograms/ml. of the yeasts studied, kluyveromyces fragilis showed the greatest sensitivity, which ranged between 0.5 and 2.5 micrograms of t-2 toxin per ml of culture medium. the roles of incubation temperature, size of the inoculum, and incubation tim ... | 1984 | 6385850 |
| adherence and penetration of vascular endothelium by candida yeasts. | metastatic infection after hematogenous dissemination of candida species is presumably dependent on the fungus traversing the vascular endothelium. an in vitro model of the earliest events of metastatic candida infection was developed with whole vascular strips. freshly obtained porcine blood vessels were secured in a perforated lucite template that allowed the application of yeasts directly to the endothelial surface. multiple wells allowed experimental and control observations on the same vasc ... | 1983 | 6352500 |
| transformation of kluyveromyces fragilis. | for the transformation of the yeast species kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (kmr) gene, the trp1 gene of saccharomyces cerevisiae, and an autonomously replicating sequence of kluyveromyces lactis called kars2 . by utilizing the method based on treatment by alkali cations and with the kmr gene as the selective marker, a wild-type strain of k. fragilis was transformed to resistance against the antibiotic g418 . in the transformed cel ... | 1984 | 6327630 |
| catabolite repression in yeasts is not associated with low levels of camp. | relationship between levels of camp and catabolite repression in yeasts has been investigated. strains of saccharomyces cerevisiae, schizosaccharomyces pombe and kluyveromyces fragilis were used. the yeasts were grown on different carbon sources to attain various degrees of repression. galactose repressed as much as glucose, while maltose was less effective. full derepression was achieved with ethanol. the enzymes tested were fructose-bisphosphatase, malate dehydrogenase, glutamate dehydrogenase ... | 1984 | 6327308 |
| sensitive bioassay for ketoconazole in serum and cerebrospinal fluid. | ketoconazole is a broad-spectrum antifungal agent which appears promising for treatment of a variety of systemic mycoses. pharmacokinetic studies are limited due to a lack of readily available methods for quantitation of ketoconazole in serum or cerebrospinal fluid. we developed a rapid, simple bioassay for measurement of ketoconazole alone or in the presence of therapeutic levels of amphotericin b, using an agar diffusion assay incorporating candida pseudotropicalis. pairs of 8-mm wells cut in ... | 1981 | 6269483 |
| cloning and expression of the structural gene for beta-glucosidase of kluyveromyces fragilis in escherichia coli and saccharomyces cerevisiae. | cellobiose, the last product in cellulose degradation, is converted into two molecules of glucose by a beta-glucosidase. s. cerevisiae does posses the structural gene for a beta-glucosidase, but it is very poorly expressed; we thus decided to isolate and characterize that of kluyveromyces fragilis. we constructed in e. coli hb101 strain a genomic library of the kluyveromyces fragilis y610 strain (atcc 12424), a yeast able to grow on cellobiose and which constitutively produces the beta-glucosida ... | 1984 | 6092839 |
| purification and phosphorylation of fructose-1,6-bisphosphatase from kluyveromyces fragilis. | fructose-1,6-bisphosphatase from the yeast kluyveromyces fragilis was found to have an apparent mr = 155,000 and to be composed of four mr = 35,000 subunits. the extent and rate of phosphorylation of fructose-1,6-bisphosphatase (fru-1,6-p2) by yeast camp-dependent protein kinase were dependent on fructose-1,6-bisphosphatase inhibitors, 5'-amp and fructose 2,6-bisphosphate (fru-2,6-p2). in the absence of inhibitor, the enzyme was slowly phosphorylated with a maximum incorporation of 1 mol of phos ... | 1984 | 6086609 |
| role of pectinolytic yeasts in the degradation of mucilage layer of coffea robusta cherries. | pectinolytic yeasts, saccharomyces marxianus, s. bayanus, s. cerevisiae var. ellipsoideus, and schizosaccharomyces sp., predominated in the natural fermentation of coffee cherries of coffea robusta variety grown in chikmagalur district of mysore state, india. these yeast species were found on the cherry surfaces, and evidence was adduced to show that the natural fermentation of coffee was the result of activity of microflora from the cherry surface itself rather than that of flora of air or wate ... | 1966 | 5959859 |
| [formation of chlamydospores of one variant of candida pseudotropicalis]. | 1966 | 5956709 | |
| [structure of the hapten from candida pseudotropicalis]. | 1965 | 5873327 | |
| biosynthesis of vitamin b6 by a yeast mutant. | the gradient-plate technique was employed to isolate mutants of saccharomyces marxianus (nrrl-y-1550) which, when grown in a synthetic culture medium, excreted about 2 mug/ml of vitamin b(6) as ascertained by microbiological assay. the major component that possessed vitamin b(6) activity was isolated by ion-exchange column chromatography and identified as pyridoxol by ultraviolet and fluorescence spectroscopy, as well as by paper chromatography and various chemical tests. pyridoxal was also iden ... | 1968 | 5732502 |
| [candida pseudotropicalis as microorganism of enzootic mastitis]. | 1967 | 5626241 | |
| cystitis due to candida pseudotropicalis. a persistent case secondary to bladder calculus. | 1969 | 5349204 | |
| kluyveromyces wikenii and kluyveromyces cicerisporus spp.n. | 1966 | 5297389 | |
| absence of photoreactivating enzyme in candida albicans. candida stellatoidea, and candida tropicalis. | in vitro assays demonstrate photoreactivating enzyme activity in extracts of candida pseudotropicalis but not in extracts of candida albicans, candida stellatoidea, or candida tropicalis. | 1974 | 4604052 |
| [respiratory metabolism of kluyveromyces fragilis van der walt]. | 1973 | 4584300 | |
| ergosterol is the only sterol in kluyveromyces fragilis. | 1974 | 4549469 | |
| spectral and biological changes induced in nicotinic acid and related compounds by ultraviolet light. | 1. irradiation of nicotinic acid, nicotinamide, nicotinamide n-oxide, n'-methyl-4-pyridone-3-carboxamide, reduced nicotinamide-adenine dinucleotide and pyridine with ultraviolet light at 253.7mmu leads to striking spectral changes. 2. nicotinic acid and nicotinamide are broken down to photosensitive intermediates which in turn undergo photodecomposition. 3. a major photoproduct of [7-(14)c]nicotinic acid is radioactive and absorbs ultraviolet light, but is inactive as a growth factor for candida ... | 1965 | 4285897 |
| [alcoholic fermentation of inulin by various strains of yeasts]. | strains of fourteen species of yeasts able to ferment inulin without previous chemical or physical hydrolysis were studied on semi-synthetic medium by evaluation of co2 production under anaerobic conditions. among them, kluyveromyces cicerisporus, candida macedoniensis and candida utilis showed the best kinetic characteristics of fermentation. experiments were carried out to specify the action of different parameters such as temperature, ph and exogenous ethanol concentration. the results obtain ... | 1985 | 4083830 |
| transfer of dna killer plasmids from kluyveromyces lactis to kluyveromyces fragilis and candida pseudotropicalis. | killer plasmids pgkl1 and pgkl2 of double-stranded linear dnas were transferred from kluyveromyces lactis to strains of kluyveromyces fragilis and candida pseudotropicalis. the resultant killer strains produced 17-fold and 6-fold larger amounts of killer toxin than k. lactis did, respectively. the killer toxin produced by each species appeared to be a glycoprotein. | 1985 | 4066615 |
| simplified bioassay method for measurement of flucytosine or ketoconazole. | a simple agar-well diffusion bioassay suitable for measurement of flucytosine or ketoconazole was developed by using candida pseudotropicalis atcc 46764 as the assay organism. a test medium composed of (per liter) 7 g of trypticase peptone, 7 g of ynb (yeast-nitrogen base), 15 g of glucose, and 15 g of agar was seeded with an inoculum which had been grown to no. 2 mcfarland turbidity after 4 to 6 h in ynb-glucose broth. determinations of flucytosine or ketoconazole were performed without necessi ... | 1985 | 4031030 |
| growth of bacteria on chitin, fungal cell walls and fungal biomass, and the effect of extracellular enzymes produced by these cultures on the antifungal activity of amphotericin b. | vibrio alginolyticus, streptomyces griseus, arthrobacter g12, bacillus sp. and cytophaga sp. were grown on solid and liquid media containing soluble and insoluble carbon sources. arthrobacter g12, bacillus sp. and cytophaga sp. grew well on media which contained fungal cell walls or fungal biomass as the main carbon source. all bacteria produced extracellular proteases and all bacteria except arthrobacter g12 produced extracellular chitinases. growth of cytophaga sp. on colloidal chitin was para ... | 1985 | 3929028 |
| normalization of monocyte candidacidal deficiency by cyclooxygenase inhibitors in hodgkin's disease. | in a previous work, the authors found that the peripheral blood monocytes from patients with hodgkin's disease (hd) had depressed lytic capability to kill candida pseudotropicalis and depressed phagocytic function. the aim of this study was to evaluate if cyclooxygenase inhibitors could correct the defective macrophage functions. fifteen untreated patients with hd and 10 normal subjects were studied. the incubation of the cells from the patients with hd with indomethacin (im) at 1, 3, and 10 mic ... | 1985 | 3922613 |
| effect of mycolase and amphotericin b on candida albicans and candida pseudotropicalis in vitro and in vivo. | a mixture of enzymes (mycolase) capable of lysing yeast cell walls was prepared from culture filtrates of physarum polycephalum. the enzymes present in mycolase included chitinase, beta-1,3-glucanases and exo-glycosidases. the ph optima of these enzymes were in the range 3.5-5.0 and they had low activities at ph 7.0. mycolase produced spheroplasts from candida pseudotropicalis and, unlike commercial enzyme preparations such as l1, chitinase, beta, 1,3-glucanase and beta-glucosidase, had some can ... | 1985 | 3895468 |
| [various characteristics of growth kinetics and exohydrolase synthesis in aspergillus foetidus and zygofabospora marxiana]. | the kinetics of growth and synthesis of exohydrolases (polygalacturonases and proteinases) were studied in aspergillus foetidus and zygofabospora marxiana. the processes of growth and synthesis of polygalacturonases were found to be shifted in time. endopolymethylgalacturonase of the micromycete and endopolygalacturonase of the yeast are not accumulated in the cells, but are mainly secreted into the growth medium. the maximal value of polygalacturonase bound to the cell coincides with the maxima ... | 1985 | 3892247 |
| evaluation of the new mycotube test-kit for yeast identification. | the performance of a modified mycotube test for the identification of yeasts was evaluated using standard biochemical tests as reference. one hundred and eighty strains belonging to 12 medically important species (15 strains each) were tested. the overall rate of identification was 72%, and rose to 87.8% when macroscopic and microscopic morphologic features were also considered; a 100% rate was obtained with candida albicans, candida krusei, candida parapsilosis, candida pseudotropicalis, candid ... | 1985 | 3886377 |
| [optimizing conditions for the discontinuous production of unicellular protein using whey]. | the cheese whey is one of the most important effluents which is being disposed off in our area. that is why the study for optimizing conditions in the process of recovering whey to produce protein biomass in a batch fermenter was undertaken. a yeast strain (kluyveromyces marxianus var. lactis) was propagated on a medium based on whey from cheese making plants, the following conditions for cell development being optimized: temperature, ph and initial concentration of lactose. a methodology based ... | 1987 | 3685391 |
| characterization of low- and high-affinity glucose transports in the yeast kluyveromyces marxianus. | glucose transport in the yeast kluyveromyces marxianus proceeds by two functionally and presumably structurally distinct transporters depending on the carbon source of the culture medium. in lactose-grown cells, glucose was taken up through a high-affinity h+-sugar symporter (km = 0.09 mm), whereas a low-affinity transporter (km = 3.5 mm) was utilized in glucose-grown cells. the two transporters exhibited different substrate specificities. galactose was demonstrated to be a selective substrate o ... | 1987 | 3663655 |
| kluyveromyces fragilis ss-437: an associatively-profiled thermotolerant yeast. | the lactose-utilizing kluyveromyces fragilis ss-437 was found to have an associative temperature profile, but a thermotolerant growth yield behaviour. cardinal growth temperatures were: 3 degrees c minimum for growth; 41.5 degrees c optimum; 44.5 degrees c final maximum (growth and death rates equalize); 46.1 degrees c initial maximum (maximum limit for growth). | 1987 | 3662480 |
| monocyte-macrophage (m-m) functions in asymptomatic hemophiliacs and supertransfused thalassemics. | patients suffering from acquired immune deficiency syndrome (aids) succumb to opportunistic infections due to a generalized failure of their cell-mediated immune defenses. the monocyte-macrophage (m-m) system plays an important role in host defense against viruses, protozoa, mycobacteria, and tumours, all potentially involved in the terminal stages of aids. we studied m-m functions in 55 asymptomatic hemophiliacs, 20 supertransfused thalassemics, and 9 von willebrand's syndrome patients over a p ... | 1987 | 3498605 |
| impairment of insulin assimilation and beta-fructosidase activity due to a petite mutation in kluyveromyces marxianus. | a respiratory deficient mutant of kluyveromyces fragilis was isolated using ethidium bromide mutagenesis. it was characterized by a loss of cytochromes a + a3 and deficiency in cytochrome b. this petite mutant has brought about modifications in the excretion pattern of beta-fructosidase active on saccharose and inulin. the mutant practically no longer excretes the enzyme, and is incapable of growth and fermentation in the presence of inulin. the study of the activities of different enzyme extrac ... | 1988 | 3395109 |
| regulation of sugar transport systems of kluyveromyces marxianus: the role of carbohydrates and their catabolism. | in kluyveromyces marxianus grown on a glucose-containing synthetic medium four different sugar transporters have been identified. in cells, harvested during the exponential phase, only the constitutive glucose/fructose carrier, probed with 6-deoxy-d-glucose or sorbose, appeared to be active. in cells from the stationary phase three proton symporters can be active, recognizing 6-deoxyglucose (a glucose/galactose carrier), sorbose (a fructose carrier) and galactosides (lactose carrier), respective ... | 1988 | 3355832 |
| [antibacterial and antifungal compounds. viii. synthesis and antifungal activity of pyrrol derivatives similar to trichostatin a]. | some p-methylbenzolpyrrole acrylic acids and related compounds were synthesized. the new pyrrole derivatives have structural features in common with trichostatin a, an antifungal antibiotic. the above acids and derivatives were tested against candida albicans and candida sp in comparison with miconazole, pyrrolnitrin and amphotericin b and showed very weak antifungal activities. occasionally some activity was found against a few strains of candida albicans and against candida pseudotropicalis. | 1987 | 3329612 |
| immunological homologies between ribosomal proteins amongst lower eukaryotes. | polyclonal antibodies were raised against the purified ribosomal proteins l1 and l2, the 5s rrna binding protein l3, all from saccharomyces cerevisiae, and against l1 and l2 from schizosaccharomyces pombe (numbering according to otaka and osawa 1981; otaka et al. 1983, respectively). for clarity prefixes sc and sp have been added to the numbering of proteins derived from s. cerevisiae and s. pombe, respectively. ribosomal proteins from these yeasts and from kluyveromyces marxianus, rhodotorula g ... | 1986 | 3327609 |
| predictive value of surveillance cultures for systemic infection due to candida species. | weekly fungal surveillance cultures (1,542 cultures) of urine (475), stool (520) and oropharyngeal (547) specimens from 111 patients on the bone marrow transplant and hematologic malignancy services were analyzed. forty-three percent of the patients were colonized by candida albicans and 10.8% by candida tropicalis. there were 22 proven systemic fungal infections, ten due to candida albicans, eight to candida tropicalis, one each to candida pseudotropicalis and torulopsis glabrata, and two to as ... | 1987 | 3326742 |
| comparison of the properties of the purified beta-glucosidase from the transformed strain of saccharomyces cerevisiae tykf2 with that of the donor strain kluyveromyces fragilis y610. | saccharomyces cerevisiae tykf2 was obtained by cloning in s. cerevisiae the gene coding for beta-glucosidase in kluyveromyces fragilis y610 (atcc 12424). the beta-glucosidases of both organisms were purified and their biochemical characteristics were determined. the two beta-glucosidases had the same enzymatic properties as those previously described in the literature. the strain s. cerevisiae tykf2 is able to produce enhanced amounts of enzyme. | 1987 | 3314909 |
| in situ assay of intracellular enzymes of yeast (kluyveromyces fragilis) by digitonin permeabilization of cell membrane. | the yeast, kluyveromyces fragilis was permeabilized to a number of low-molecular-weight substrates using digitonin. the activities of intracellular yeast enzymes, viz., alcohol dehydrogenase (adh), beta-galactosidase, glucose-6-phosphate dehydrogenase, aspartase, and hexokinase were found to be much higher in the permeabilized cells than the untreated cells. the optimum conditions for permeabilization with reference to adh were 0.1% digitonin at 37 degrees c for 15 min. the adh activity in perme ... | 1988 | 3149161 |
| the promoter of the beta-glucosidase gene from kluyveromyces fragilis contains sequences that act as upstream repressing sequences in saccharomyces cerevisiae. | the relationship between the promoter length of the kluyveromyces fragilis beta-glucosidase gene and the level of its expression in saccharomyces cerevisiae was studied by gene fusion between deleted promoter fragments of various lengths and the promoterless beta-galactosidase gene of escherichia coli. the removal of a region from position -425 to -232 led to a tenfold increase in the expression of the gene. the same results were obtained for the reconstructed beta-glucosidase gene with the same ... | 1988 | 3146687 |
| biosynthesis regulation of the beta-glucosidase produced by a yeast strain transformed by genetic engineering. | the biosynthesis of the beta-glucosidase enzyme was studied in a transformed yeast obtained by cloning in saccharomyces cerevisiae the structural gene coding for beta-glucosidase in kluyveromyces fragilis. the enzyme biosynthesis was found to be non-adaptative, and repressed by glucose. these features are similar to those observed in k. fragilis. beta-glucosidase activity in the transformed yeast was much higher than in k. fragilis. we attempted to ferment cellobiose with the transformed yeast: ... | 1986 | 3099720 |
| lysosomal enzyme release from macrophages: a model of food yeast toxicity evaluation. | the role of lysosomal enzyme released by macrophages was examined in relation to the toxic effect caused by food yeast. mouse peritoneal macrophages exposed to yeast in culture showed marked release of n-acetyl glucosaminidase, beta-galactosaminidase and beta-glucuronidase below the median lethal dose (ld50). ld50 was measured from the dose response curves of the cytoplasmic lactate dehydrogenase enzyme. saccharomyces cerevisiae showed the highest ld50 followed by kluyveromyces fragilis and cand ... | 1988 | 3076734 |
| ofage banding patterns of different yeast genera and of intergeneric hybrids. | electrophoretic karyotypes of yeasts belonging to the species saccharomyces cerevisiae, kluyveromyces marxianus and candida macedoniensis were established by means of ofage. hybrids between s. cerevisiae and k. marxianus as well as between k. marxianus and c. macedoniensis were analyzed by comparing their ofage-banding-pattern with the parental banding-patterns. thus, evidence for exchanges of intact chromosomes and for chromosomal rearrangements could be gained on a molecular level. | 1988 | 3057175 |
| the role of atp in the control of h+-galactoside symport in the yeast kluyveromyces marxianus. | transport of methyl beta-d-thiogalactoside and p-nitrophenyl beta-d-galactoside is shown to proceed through the h+-lactose symporter of kluyveromyces marxianus. uptake of these compounds is strongly reduced under anaerobic conditions or aerobically in the presence of antimycin. it is shown that antimycin treatment affects p-nitrophenyl beta-d-galactoside uptake in a similar way as it affects the cellular amount of atp, suggesting regulation of p-nitrophenyl beta-d-galactoside transport by atp. a ... | 1987 | 3036099 |
| molecular probe for identification of medically important candida species and torulopsis glabrata. | a cloned dna fragment from candida albicans containing the gene for the protein actin was used to probe the molecular structure of the actin gene of several medically important yeasts (c. albicans, candida stellatoidea, candida tropicalis, candida pseudotropicalis, candida krusei, candida parapsilosis, candida guilliermondii, and torulopsis glabrata). whole-cell dna from each species was digested with restriction endonucleases, electrophoresed on agarose gels, and transferred to nitrocellulose. ... | 1987 | 3033014 |
| mitochondrial introns as mobile genetic elements: the role of intron-encoded proteins. | introns of organelle genes share distinctive rna secondary structures that allow their classification into two known families. these structures are believed to play an essential role in splicing, and members of both structural classes have recently been shown to perform self-splicing reactions in vitro. in lower eukaryotes, many structured introns also contain long internal open reading frames (orfs), which are able to code for hydrophilic proteins. several properties of self-splicing structured ... | 1986 | 3032144 |
| early defect of phagocytic cell function in subjects at risk for acquired immunodeficiency syndrome. | we studied the functions of peripheral blood monocytes and polymorphonuclear cells in 15 apparently healthy homosexual men, eight homosexual or bisexual subjects with unexplained generalized lymphadenopathies (pre-aids), four homosexual men with acquired immunodeficiency syndrome (aids), and 15 heterosexual men. in comparison with normal controls, the homosexual groups studied presented a decreased monocyte candidacidal activity for candida pseudotropicalis that gradually deteriorates as the cli ... | 1986 | 3018916 |
| arrangement of genes trp1 and trp3 of saccharomyces cerevisiae strains. | the tryptophan biosynthetic genes trp1 and trp3 and partly also trp2 and trp4 have been compared by the technique of southern hybridization and enzyme measurements in twelve wild isolates of saccharomyces cerevisiae from natural sources of different continents, in the commonly used laboratory strain s. cerevisiae x2180-1a and in a kluyveromyces marxianus strain. we could classify these strains into four groups, which did not correlate with their geographical distribution. in no case are the trp3 ... | 1985 | 2998296 |
| mitochondrial dna of the yeast kluyveromyces: guanine-cytosine rich sequence clusters. | mitochondrial dna from the yeast kluyveromyces marxianus var. lactis (k.lactis) is a circular molecule of 39 kilobase-pairs. a genetic and physical map was constructed. we found that this genome contained a large number of guanine-cytosine (gc)-rich sequence clusters, many of which are characterized by the presence of sacii restriction sites (ccgcgg). the primary sequence of the gc clusters often showed a palindromic structure. these gc clusters were present in several varieties of k.marxianus, ... | 1988 | 2843818 |
| sequence and transcription of the beta-glucosidase gene of kluyveromyces fragilis cloned in saccharomyces cerevisiae. | the complete nucleotide sequence of the beta-glucosidase gene of kluyveromyces fragilis has been determined. this sequence contains an open reading frame of 2535 base pairs encoding a protein of 845 amino acids. analysis of the transcription products revealed only one transcript of about 3 kb identical in both kluyveromyces fragilis and in the expression host saccharomyces cerevisiae. the protein molecular weight of 93,811 kd deduced from the sequence is consistent with the 90,000 kd determined ... | 1987 | 2835179 |
| synthesis of some new mannich bases derived from 6-acyl-3-(3,5-dimethylpiperidinomethyl)-2(3h)-benzoxazolones+ ++ and their biological activities. | a number of 6-acyl-3-(3,5-dimethylpiperidinomethyl)-2(3h)-benzoxazolones have been synthesized by mannich reaction and their antibacterial and antifungal activities have been tested. their chemical structures have been proved by means of their ir, nmr, mass spectroscopic data and by elementary analysis. investigation of antimicrobial activity of compounds was done by tube dilution and paper disc techniques using bacteria (escherichia coli atcc 25922, staphylococcus aureus atcc 25923, pseudomonas ... | 1989 | 2818673 |
| ester formation from ethanol by candida pseudotropicalis. | the production of ethanol, acetate ion and ethyl acetate from glucose by the yeast candida pseudotropicalis ncyc 143 was investigated under aerobic and anaerobic growth conditions. acetate and ethyl acetate only accumulated under aerobic conditions, whereas production of the alcohol was favoured by anaerobic conditions. ester production during aerobic growth was enhanced substantially by growth in iron-deficient media. possible conditions for optimising ester production from ethanol in dilute pr ... | 1989 | 2802574 |
| evaluation of cilofungin, a lipopeptide antifungal agent, in vitro against fungi isolated from clinical specimens. | cilofungin (ly121019) is a new lipopeptide antifungal drug. we tested this drug against 141 pathogenic fungal isolates. all fungal species were tested by broth dilution at 35 degrees c. malassezia furfur was tested by agar dilution. the results demonstrate the specificity of cilofungin activity. candida albicans, candida tropicalis, and malassezia pachydermatis were highly susceptible, whereas candida parapsilosis, candida pseudotropicalis, candida krusei, torulopsis glabrata, blastomyces dermat ... | 1989 | 2802565 |
| induction of murine macrophage fungal killing by interleukin 3. | the effect of recombinant interleukin 3 (il-3) on the function of murine resident peritoneal macrophages was investigated. il-3 enhanced the phagocytosis of candida pseudotropicalis and candida albicans and enhanced killing of the former. the enhanced killing is inhibited by scavengers of oxygen radicals, suggesting that il-3 primes macrophages for enhanced oxidative metabolism in response to candida. | 1989 | 2788580 |
| restriction endonuclease analysis of mitochondrial dna from candida parapsilosis and other candida species. | mitochondrial dna has been studied in a number of eukaryotic organisms. differences in inter- and intraspecies mitochondrial dna restriction patterns have been shown to be due to differences in nucleotide sequences and have been used to study evolutionary relationships and the mode of inheritance of the mitochondrial genome. a relatively rapid and efficient method for the extraction of mitochondrial dna from candida parapsilosis and other candida species was developed. zymolyase was used to indu ... | 1989 | 2665367 |
| polyphosphate synthesis in yeast. | polyphosphate synthesis was studied in phosphate-starved cells of saccharomyces cerevisiae and kluyveromyces marxianus. incubation of these yeasts for a short time with phosphate and either glucose or ethanol resulted in the formation of polyphosphate with a short chain length. with increasing incubation times, polyphosphates with longer chain lengths were formed. polyphosphates were synthesized faster during incubation with glucose than with ethanol. antimycin did not affect the glucose-induced ... | 1989 | 2643440 |
| synthesis and biological activity of 6-acyl-3-substituted-2(3h)-benzoxazolones. | the preparation of a series of 6-acyl-3-substituted-2-(3h)-benzoxazolones is described. their structures have been elucidated by uv, ir, nmr, ms and elementary analysis. these compounds have been tested for their antimicrobial activities against to escherichia coli atcc 25922, staphylococcus aureus, atcc 25923, pseudomonas aeruginosa atcc 27853, staphylococcus faecalis rskk 10541, candida parapsilosis, candida albicans, candida pseudotropicalis, candida stellatoidea. | 1989 | 2621552 |
| ammonium assimilation by candida albicans and other yeasts: evidence for activity of glutamate synthase. | activities and properties of the ammonium assimilation enzymes nadp+-dependent glutamate dehydrogenase (gdh), glutamate synthase (gogat) and glutamine synthetase (gs) were determined in batch and continuous cultures of candida albicans. nadp+-dependent gdh activity showed allosteric kinetics, with an s0.5 for 2-oxoglutarate of 7.5 mm and an apparent km for ammonium of 5.0 mm. gogat activity was affected by the buffer used for extraction and assay, but in phosphate buffer, kinetics were hyperboli ... | 1989 | 2575653 |
| molecular probes for the detection of kluyveromyces marxianus chromosomal dna in electrophoretic karyotypes of intergeneric protoplast fusion products. | random genomic dna fragments from kluyveromyces marxianus were cloned in order to identify chromosomal bands in pulsed field electrophoresis patterns of intergneric hybrid strains which were obtained by protoplast fusion. molecular hybridization data indicated that the k. marxianus parental strain might be triploid, and it showed strong chromosome length polymorphism. we analyzed the karyotype of two saccharomyces cerevisiae/k. marxianus hybrid strains (st. 1.st.46) with our dna probes and with ... | 1989 | 2573327 |
| [in vitro activity of fluconazole, a novel bistriazole antifungal agent]. | fluconazole is a novel triazole antifungal agent with excellent activities against a broad range of medically important fungi. the in vitro antifungal activities of fluconazole especially against candida albicans were examined and the results summarized as follows: 1. fluconazole was proved to exhibit the highest antifungal activity in synthetic amino-acid medium, fungal (saamf), well buffered in a physiologically neutral range. 2. in a exposed time-killing test performed using saamf (ph 7.4), t ... | 1989 | 2540356 |
| [the search for microorganisms sensitive to deoxynivalenol, zearalenone and aflatoxin b1]. | sensitivity to t-2 toxin, deoxynivalenol (dov), zearalenon (zl) and aflatoxin (at) b1 was comparatively studied in yeast strains saccharomyces fragilis 25 d, candida pseudotropicalis 44 nk and saccharomyces lactis bkmy-459, sensitive to t-2 toxin, and strain bacillus megaterium bkmb-44, sensitive to toxic metabolites of aspergillus species of fungi. minimum amounts of t-2 toxin inducing on silufol plates sites of the yeast strain growth suppression, comprised 20-50 ng, maximum sensitivity was re ... | 1989 | 2534250 |