Publications

in vitro germination, protocorm formation and plantlet development of mature versus immature seeds from several ophrys species (orchidaceae).	we investigated the effect of genotype, seed maturity and culture medium on the in vitro germination and development of protocorms and plantlets from seeds of 13 different ophrys species (o. apifera, o. attica, o. cornuta, o. delfinensis, o. ferrum-equinum, o. lutea, o. mammosa, o. speculum, o. spruneri, o. umbilicata, o. argolica, o. irricolor and o. tenthredinifera) collected in greece, some of which are endemic to this country. mature seeds (10 months after collection) and immature seeds (2 m ...	2004	15517276
[most common allergens in allergic patients admitted into a third-level hospital].	since year 3000 bc, pollenology (science that studies allergens) was already known. in the year 49 bc, herodotus recognized the first case of hay fever, jacob constant (17th century) described the first case of allergic rhinitis and related it to "something" (allergens) that roses emitted. charles backley (1873) proved the ability of the skin to determine allergic reactions to allergens. today, it is well-known that allergens are able to react with environmental exposure, plants and pollens.	2004	15491079
physicochemical characterization of a new pineapple hybrid (flhoran41 cv.).	the physicochemical characteristics (ph, total and soluble solids, and titratable acidity), sugars, organic acids, carotenoids, anthocyanins, volatile compounds, and cell wall polysaccharides of a new pineapple hybrid (flhoran41 cultivar) were measured throughout maturation and compared with the smooth cayenne cv. at full maturity, the flhoran41 cv. has a higher titratable acidity and soluble solids content than the smooth cayenne cv. the golden yellow flesh and red-orange to scarlet shell of ri ...	2004	15453683
pineapple plantations' prudential plan.		1950	15428097
nutrient composition and antimicrobial activity of sorrel drinks (soborodo).	aqueous extracts (1,200 ml) of roselle calyx (40 g), fortified with either orange juice or pineapple juice as sweetener and lemon grass as flavorant (sorrel drink), were analyzed with regard to their mineral composition (na, fe, zn, cu, pb, mn, and ca), vitamin c content, and sensory evaluation. while the medicinal potentials were determined with respect to their inhibitory effect on the growth of bacillus sp., pseudomonas aeruginosa, lactobacillus sp., and corynebacterium sp. the results reveal ...	2004	15383229
evaluation of anthelmintic properties of some plants used as livestock dewormers against haemonchus contortus infections in sheep.	gastrointestinal helminth infections remain a major constraint to livestock production globally. this study evaluated anthelmintic efficacy of 7 plants used as dewormers by farmers and pastoralists in kenya. thus 3 commercial anthelmintics and 7 plant preparations were tested in lambs infected with 5000 or 3000 l3 haemonchus contortus in 4 experiments. in the first experiment, ivermectin, levamisole and albendazole were tested in 46 lambs. seven plant preparations of hagenia abyssinica, olea eur ...	2004	15376783
respiratory properties and malate metabolism in percoll-purified mitochondria isolated from pineapple, ananas comosus (l.) merr. cv. smooth cayenne.	an investigation was made of the respiratory properties and the role of the mitochondria isolated from one phosphoenolpyruvate carboxykinase (pck)-cam plant ananas comosus (pineapple) in malate metabolism during cam phase iii. pineapple mitochondria showed very high malate dehydrogenase (mdh), and low malic enzyme (me) and glutamate-oxaloacetate transaminase (got) activities. the mitochondria readily oxidized succinate and nadh with high rates and coupling, while they only oxidized nadph in the ...	2004	15361538
molecular cloning, expression, and functional characterization of a cystatin from pineapple stem.	a cdna fragment encoding the cysteine protease inhibitor, cystatin, was cloned from pineapple (ananas comosus) stem. this clone was constructed in a fusion vector and was easily over-expressed in escherichia coli; satisfactory over-expression of non-fusion cystatin was achieved after an additional start codon was inserted prior to its coding sequence. both recombinant cystatins were predominately found in the soluble fraction of the cell extract, and were demonstrated to be functionally active i ...	2004	15322351
[test for urokinase-type plasminogen activator inhibitor of edible plants in vitro].	to study the effect of anti-progression activity of edible plants using urokinase as the biomarker.	2004	15312585
sensitization to ficus benjamina: relationship to natural rubber latex allergy and identification of foods implicated in the ficus-fruit syndrome.	ornamental ficus benjamina (fb) has been recognized as a new indoor allergen. little is known about the prevalence in moderately exposed subjects and the proposed association with fruit and hevea latex hypersensitivity.	2004	15298566
determination of the 13c/12c ratio of ethanol derived from fruit juices and maple syrup by isotope ratio mass spectrometry: collaborative study.	a collaborative study of the carbon-13 isotope ratio mass spectrometry (13c-irms) method based on fermentation ethanol for detecting some sugar additions in fruit juices and maple syrup is reported. this method is complementary to the site-specific natural isotope fractionation by nuclear magnetic resonance (snif-nmr) method for detecting added beet sugar in the same products (aoac official methods 995.17 and 2000.19), and uses the same initial steps to recover pure ethanol. the fruit juices or ...	2004	15287660
day-night changes of energy-rich compounds in crassulacean acid metabolism (cam) species utilizing hexose and starch.	plants with crassulacean acid metabolism (cam) can be divided into two groups according to the major carbohydrates used for malic acid synthesis, either polysaccharide (starch) or monosaccharide (hexose). this is related to the mechanism and affects energy metabolism in the two groups. in kalanchoë pinnata and k. daigremontiana, which utilize starch, atp-dependent phosphofructokinase (tonoplast inorganic pyrophosphatase) activity is greater than inorganic pyrophosphate-dependent phosphofructokin ...	2004	15277250
identification of carbohydrate degrading bacteria in sub-tropical regions.	several bacteria from the islands of puerto rico, vieques and trinidad were isolated for their carbohydrate degrading activities. these terrestrial and marine bacterium were collected from pineapple agricultural lands, tropical rain forests, coastlands and mangrove swamps. organisms were screened for activity using chromogenic substrates (azcl megazyme international ltd., ireland). the media composition for the effective culturing of some of the marine organisms has also been standardized. gram- ...	2003	15264573
intra-oral acid production associated with eating whole or pulped raw fruits.	the hypotheses that raw fruits, whether whole or pulped, were cleared rapidly from the mouth and that the sugars in the whole and pulped fruits are fermented with equal efficiency to acids by the oral microflora were tested in this study. groups of 7-9 adult subjects chewed 10 g of raw, whole or pulped fruit (apple, banana, orange, pear and pineapple) for 1 min and whole, unstimulated saliva samples were collected during the following 60-min interval. each saliva sample was assayed for the conce ...	2016	15181333
bromelain partitioning in two-phase aqueous systems containing peo-ppo-peo block copolymers.	bromelain is an enzymatic complex obtained from pineapple (ananas comosus) fruits and stem. thermoseparation of bromelain by poly(ethylene oxide) (peo)- poly(propylene oxide) (ppo)- poly(ethylene oxide) (peo) block copolymers aqueous solutions was studied. triblock copolymers with different eo percentages and different molecular mass were evaluated. copolymer solutions at different ph values, buffer concentrations and copolymer concentrations were investigated. it was found that cloud point temp ...	2004	15177161
effect of carbohydrate source on post-prandial blood glucose in subjects with type 1 diabetes treated with insulin lispro.	our purpose was to determine if the glycemic index (gi) and proportion of carbohydrate absorbed as glucose (pg) affected glycemic responses and occurrence of post-prandial hypoglycemia in subjects with type 1 diabetes treated with insulin lispro. subjects (n=8) were studied on five separate occasions after 10-12 h overnight fasts following a standard dinner. after their morning insulin dose, subjects ate 50 g carbohydrate from a starchy food (pg=1; mashed potato gi=83, white bread gi=71, spaghet ...	2004	15163475
the effect of learning on experimental evolution of resource preference in drosophila melanogaster.	learning is thought to be adaptive in variable environments, whereas constant, predictable environments are supposed to favor unconditional, genetically fixed responses. a dichotomous view of behavior as either learned or innate ignores a potential evolutionary interaction between the learned and innate components of a behavioral response. we addressed this interaction in the context of oviposition substrate choice in drosophila melanogaster, asking two main questions. first, will learning also ...	2004	15154552
two low-cost food attractants for capturing toxotrypana curvicauda (diptera: tephritidae) in the field.	adults of the papaya fruit fly, toxotrypana curvicauda gerstaecker, living in wild vegetation in yautepec, morelos, mexico, were captured using mcphail traps baited with pineapple juice or brown sugar. maximum capture peaks occurred during august and november in the afternoon. baits were most effective when aged over a 4-d period. traps baited with brown sugar captured the highest number of adults, and both baits were more attractive to females than males. potential use of these baits for pest m ...	2004	15154449
effect of acid speciation on solid waste liquefaction in an anaerobic acid digester.	laboratory scale experiments were carried out to evaluate and determine the suitable working conditions for the enhancement of volatile fatty acids (vfas) production with the stabilization of solid pineapple waste (peel) simultaneously when operating two-phase anaerobic digestion. it was found that acid production, enhanced when the digester was operated at neutral ph. vfas, could be obtained at about 50 g/kg-waste within 14 days when operating at ph 6.5-7.5 compared to 33 g/kg-waste for digeste ...	2004	15142803
flavor analysis in a pharmaceutical oral solution formulation using an electronic-nose.	flavors are commonly used in pharmaceutical oral solutions and oral suspensions to mask drug bitterness and to make the formulation more palatable. flavor analysis during product development is typically performed by human organoleptic analysis, which is often expensive and less objective. a novel approach using a metal oxide sensor-based instrument (electronic-nose) for headspace analysis was explored to replace human sensory perception for consistent qualitative and quantitative analysis of fl ...	2004	15127800
ensilage of pineapple processing waste for methane generation.	pineapple peel wastes, which are seasonal, comprise of peels and rags. their disposal poses a serious environmental pollution problem. since pineapple peel is rich in cellulose, hemicellulose and other carbohydrates it was found to be a potential substrate for methane generation by anaerobic digestion. ensilaging of pineapple peel resulted in the conversion of 55% carbohydrates into volatile fatty acids. the ensilage of pineapple processing wastes reduced the biological oxygen demand by 91%. bio ...	2004	15120436
sulfur dioxide effects on plants exhibiting crassulacean acid metabolism.	the effects of so(2) on species exhibiting crassulacean acid metabolism (cam) were determined with short term-high concentration 'acute' greenhouse exposures (0.6 to 3.0 microl liter(-1) (ppm) so(2) for 2 and 8 h), and long term-low concentration 'chronic' field exposures (0.35 to 0.90 microl liter(-1) so(2) for 32 to 79 h periodically over 7 to 13 days). in the acute greenhouse exposures, visible injury was observed on opuntia basilaris engelm. & bigel., exposed to 2.0 microl liter(-1) so(2), b ...	1987	15092814
purification and characterization of a pineapple crown leaf thiol protease.	a thiol protease was isolated and purified from the crown leaf of pineapple, ananas comosus (l.) merr. cv. queen, by an immunoaffinity procedure. after the purification to electrophoretic homogeneity, the enzyme was characterized with respect to some of its physico-chemical and kinetic properties. the molecular weight of the protease (22.4-22.9 kda), km (97 microm) and kcat (8.8 s(-1)) for its esterolytic cleavage of the synthetic protease substrate n(alpha)-cbz-l-lysine p-nitrophenyl ester, the ...	2004	15046295
proteolytic activity and immunogenicity of oral bromelain within the gastrointestinal tract of mice.	bromelain is a mixture of proteinases derived from pineapple stem that is marketed by health food stores as a "digestive aid". a number of studies suggest that bromelain may also have anti-inflammatory activity in vivo, including an anecdotal report describing potential efficacy in inflammatory bowel disease. we and others have previously shown that proteolytically active bromelain removes certain cell surface molecules and affects leukocyte migration, activation, and production of cytokines and ...	2004	14996417
determination of ochratoxin a in currants, raisins, sultanas, mixed dried fruit, and dried figs by immunoaffinity column cleanup with liquid chromatography: interlaboratory study.	an interlaboratory study was performed on behalf of the food standards agency to evaluate the effectiveness of an affinity column cleanup liquid chromatographic (lc) method for the determination of ochratoxin a in a variety of dried fruit at european regulatory limits. to ensure homogeneity before analysis, laboratory samples are normally slurried with water in the ratio of 5 parts fruit to 4 parts water, and test materials in this form were used in the study. the test portion was extracted with ...	2012	14979698
composition of honeydew excreted by pineapple mealybugs.		1952	14913190
pineapple dermatosis.		1951	14895783
individual variation of the fruits of the pineapple (ananas comosus l. merr.) in regard to certain constituents of the juice.		2016	14840519
quantitative prediction of the bitterness suppression of elemental diets by various flavors using a taste sensor.	the purpose of the study was to develop a method for the quantitative prediction of the bitterness suppression of elemental diets by various flavors and to predict the optimum composition of such elemental diets for oral administration using a multichannel taste sensor.	2003	14725356
the pineapple eye gene is required for survival of drosophila imaginal disc cells.	each ommatidium of the drosophila eye is constructed by precisely 19 specified precursor cells, generated in part during a second mitotic wave of cell divisions that overlaps early stages of ommatidial cell specification. homozygotes for the pineapple eye mutation lack sufficient precursor cells due to apoptosis during the period of fate specification. in addition development is delayed by apoptosis during earlier imaginal disc growth. null alleles are recessive lethal and allelic to l(2)31ek; h ...	2003	14704172
on-line gas chromatography combustion/pyrolysis isotope ratio mass spectrometry (hrgc-c/p-irms) of pineapple (ananas comosus l. merr.) volatiles.	by use of extracts prepared by liquid-liquid separation of the volatiles from self-prepared juices of pineapple fruits (ananas comosus) (n = 14) as well as commercial pineapple recovery aromas/water phases (n = 3), on-line capillary gas chromatography-isotope ratio mass spectrometry was employed in the combustion (c) and the pyrolysis (p) modes (hrgc-c/p-irms) to determine the delta(13)c(vpdb) and delta(2)h(vsmow) values of selected pineapple flavor constituents. in addition to methyl 2-methylbu ...	2003	14690391
[protein hydrolysate as a dietetic resource]	objective: the central goal of this paper was to study the application of beef meat and poultry (turkey and chicken) hydrolysates to the preparations used in our brazilian current feeding practices. methods: the various kinds of meat were hydrolyzed with fresh pineapple under similar conditions to those daily used at home. the selection of three types of preparation was dependent on whether their contents included starch or gelatin and liquid, like soup, mousse and fruit-shake. hydrolysate were ...	1998	14685623
tropgene-db, a multi-tropical crop information system.	tropgene-db, is a crop information system created to store genetic, molecular and phenotypic data of the numerous yet poorly documented tropical crop species. the most common data stored in tropgene-db are information on genetic resources (agro-morphological data, parentages, allelic diversity), molecular markers, genetic maps, results of quantitative trait loci analyses, data from physical mapping, sequences, genes, as well as the corresponding references. tropgene-db is organized on a crop bas ...	2004	14681435
occupational exposure of brazilian neonatal intensive care workers to latex antigens.	frequent exposure to latex causes various reactions such as respiratory symptoms and anaphylactic shock. in these cases, proteins found in natural latex are responsible for the serious systemic antilatex-mediated immediate hypersensitive reactions.	2004	14674942
a sensitized genetic screen to identify novel regulators and components of the drosophila janus kinase/signal transducer and activator of transcription pathway.	the jak/stat pathway exerts pleiotropic effects on a wide range of developmental processes in drosophila. four key components have been identified: unpaired, a secreted ligand; domeless, a cytokine-like receptor; hopscotch, a jak kinase; and stat92e, a stat transcription factor. the identification of additional components and regulators of this pathway remains an important issue. to this end, we have generated a transgenic line where we misexpress the upd ligand in the developing drosophila eye. ...	2003	14668372
relationships in ananas and other related genera using chloroplast dna restriction site variation.	chloroplast dna (cpdna) diversity was examined using pcr-rflp to study phylogenetic relationships in ananas and related genera. one hundred fifteen accessions representing the seven ananas species and seven other bromelioideae including the neighboring monospecific genus pseudananas, two pitcairnioideae, and one tillandsioideae were included in the study. eight primers designed from cpdna were used for generating fragments. restriction by 18 endonucleases generated 255 variable fragments. dissim ...	2003	14663518
effect of ph, temperature and alcohols on the stability of glycosylated and deglycosylated stem bromelain.	the biological significance of the carbohydrate moiety of a glycoprotein has been a matter of much speculation. in the present work, we have chosen stem bromelain from ananas comosus as a model to investigate the role of glycosylation of proteins. stem bromelain is a thiol protease which contains a single hetero-oligosaccharide unit per molecule. here, the deglycosylated form of the enzyme was obtained by periodate oxidation. the differences in the glycosylated and deglycosylated forms of the gl ...	2003	14660870
tetrahydro-beta-carboline alkaloids occur in fruits and fruit juices. activity as antioxidants and radical scavengers.	tetrahydro-beta-carbolines are biologically active alkaloids that occur and accumulate in mammalian tissues, fluids, and brain, but their ultimate origin or biological role is still uncertain. four tetrahydro-beta-carboline alkaloids: 1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid, 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid, 1-methyl-1,2,3,4-tetrahydro-beta-carboline, and 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline, are found as naturally occurring substances in s ...	2003	14611187
the anthelmintic efficacy of five plant products against gastrointestinal trichostrongylids in artificially infected lambs.	forty-eight helminth-free lambs were divided into eight groups (a-h) of six animals. groups a-g were infected artificially with 10,000 third stage larvae of haemonchus contortus and 20,000 third stage larvae of trichostrongylus colubriformis, whereas group h remained uninfected. thirty days post-infection the lambs were treated orally with a single dosage of one of the following products: group a with 3 mg/kg body weight (bw) of an aqueous ethanol extract (70%, v/v) of the seeds of azadirachta i ...	2003	14597279
environmental, life-style, and physical precursors of clinical parkinson's disease: recent findings from the honolulu-asia aging study.	increased westernization with japanese migration to the u. s. in the early 20(th) century is thought to have altered the risk of cardiovascular disease. whether similar effects include changes in the risk of parkinson's disease (pd) is not clear. this report describes the relations between environmental, life-style, and physical attributes and the incidence of pd that have been observed in the honolulu-asia aging study.	2003	14579122
enzymatic browning and biochemical alterations in black spots of pineapple [ananas comosus (l.) merr.].	penicillium funiculosum thom. was consistently isolated from pineapple-infected fruitlet (black spots). polyphenol oxidase, peroxidase, and laccase activities were determined in extracts from contiguous and infected fruitlets. healthy fruitlets showed a rather high level of polyphenol oxidase (optimum ph 7.0), and this activity was tremendously increased (x 10) in contiguous infected fruitlets. furthermore, infected fruitlets also exhibited laccase activity (optimum ph 4.0), while peroxidase was ...	2003	14506857
"pineapple slice dressing" for correction of inverted nipples.		2003	14504562
survival of escherichia coli o157:h7, listeria monocytogenes, and salmonella in juice concentrates.	the survival of escherichia coli o157:h7, listeria monocytogenes, and salmonella was studied in apple, orange, pineapple, and white grape juice concentrates and banana puree. pouches of juice concentrate or puree were inoculated with pathogens at a level > or = 10(3) cfu/g and stored at -23 degrees c (-10 degrees f). pathogen survival was monitored at 6 and 24 h, once a week for four consecutive weeks, and biweekly thereafter until 12 weeks. when pathogens were not detectable by direct plating, ...	2003	14503711
[bromelin, a protease of pineapple with antiedema action (ananase)].		1965	14293137
tryptamine oxidation by extracts of pea seedlings: effect of growth retardant beta-hydroxyethylhydrazine.	beta-hydroxyethylhydrazine forces flowering of pineapple plants, possibly by reducing auxin concentration. a specific and sensitive assay showed that beta-hydroxyethylhydrazine (3.3 x 10(-7)m) inhibits noncompetitively and almost completely the oxidation of tryptamine to indoleacetaldehyde by extracts of pea-seedling (pisum sativum l. var. alaska). the relative rate of oxidation of putrescine to delta(1)-pyrroline compared to that of tryptamine by these extracts and the inhibition of both reacti ...	1965	14289611
orally induced fibrinolysis. a critical assessment of pineapple protease.		1964	14254697
pineapple proteases in the treatment of scleroderma; a case report.		1964	14143037
the coenzyme requirement and enzyme inhibitors of pineapple indoleacetic acid oxidase.		1958	13575446
[erythema multiforme caused by allergy to pineapple; case report].		1957	13432316
dermatological hazards of the pineapple industry.		2006	13428186
prevention of eye accidents in sugar and pineapple industries of hawaii.		1956	13285929
[treatment of cervical erosions with siberian pineapple oil].		2016	13282819
indoleacetic acid oxidase and an inhibitor in pineapple tissue.		1953	13117833
ascorbic acid and physiological breakdown in the fruits of the pineapple (ananas comosus l. merr).		1952	13015097
pineapple juice as an agent for the digestion of fish prior to the harvesting of metacercariae.	the efficacy of crude pineapple juice obtained from ananus comosus in digesting fish for the harvesting of trematode metacercariae was investigated. no significant difference was found between the total number of metacercariae detected from fish (cirrhina jullieni) digested by acid pepsin and those digested by freshly prepared pineapple juice that was kept for 15 days at a temperature of either -4 degrees c or -75 degrees c. however, fewer metacercariae were found when using juice that had been ...	2002	12971486
trace-level determination of pesticides in food using difficult matrix introduction-gas chromatography-time-of-flight mass spectrometry.	a procedure for the fully automated analysis of food samples by means of difficult matrix introduction-gas chromatography-time-of-flight mass spectrometry (dmi-gc-tof ms) is discussed. after extraction, samples require very little clean-up and are injected in a micro- or micro-vial which is held in a liner. next, the liner is placed in the injector and the contents of the vial are thermally desorbed and led directly to the capillary gc column. after gc-tof ms analysis, the data are processed aut ...	2003	12967188
the prevalence and diagnostic value of specific ige antibodies to inhalant, animal and plant food, and ficus allergens in patients with natural rubber latex allergy.	it is well recognised that natural rubber latex allergy can be associated with serological cross-reactivity to plant allergens, especially tropical fruits and ficus. in contrast, data on the frequency and clinical value of specific ige antibodies against these allergens remain rare. in addition, little is known about the prevalence and diagnostic value of specific ige antibodies to classical inhalant and animal allergens in nrl allergic patients.	2003	12945478
lactic acid production using two food processing wastes, canned pineapple syrup and grape invertase, as substrate and enzyme.	canned pineapple syrup, a food processing waste, was utilized as a substrate for lactic acid production by lactococcus lactis. to improve the utilization of sucrose from the syrup, grape invertase from grape juice derived from wine production was used for sucrose hydrolysis. the highest lactic acid concentrations achieved were 20 and 92 g l-1 from 20 and 100 g total sugars l-1, respectively, without a lag period for sucrose consumption.	2003	12882147
cross-reactivity between ficus benjamina latex and fig fruit in patients with clinical fig allergy.	anaphylactic reactions to fig fruits (ficus carica) have been reported from subjects sensitized to ficus benjamina (fb) latex allergens. figs may also be involved in the latex-fruit syndrome.	2003	12859455
cost-effective in vitro propagation methods for pineapple.	we have developed an efficient and cost-effective method for commercial micropropagation of smooth cayenne pineapple. in vitro shoots were used as starting materials, and either longitudinal sections of the shoots or leaf bases were used as the explants to regenerate shoots. when these explants were used, the axillary meristems, which usually remain quiescent during shoot multiplication, were able to form new shoots. subsequent to the regeneration step, additional multiplication was achieved ins ...	2003	12789501
[genetic variation of citrus calli revealed by the ploidy analyser].	the cell dna content of forty-eight citrus calli of different genotype were measured by using the ploidy analyser. the results showed that 93.8% out of forty-eight citrus calli had double dna content except that ruby. weizhang and kinnow had little varied cells and did not show second peak. moreover, pineapple, meiwa kumquat, changsha, russ navel, guoqing no. 4 and carter had dna varied cells including triploids and aneuploids based on the dpac software analyse. among the forty-eight citrus call ...	2003	12776606
determination of antioxidant properties of aromatic herbs, olives and fresh fruit using an enzymatic sensor.	the aim was to experimentally evaluate the antioxidant capacity of different fresh aromatic herbs (field balm, marjoram, parsley, rosemary, sage, sweet basil), several varieties of olives from central italy ('carboncello', 'rosciolo', 'olivastro', 'coratello', 'leccino', 'frantoio') and several types of fresh fruit (apple, apricot, banana, cherry, fig, grape, medlar, melon, peach, pear, pineapple, plum, water melon, yellow plum) using a superoxide dismutase (sod) biosensor developed by the prese ...	2003	12733012
identifying the pattern of olfactory deficits in parkinson disease using the brief smell identification test.	selective olfactory deficits occur in 70% to 90% of patients with parkinson disease, independent of disease severity and duration. olfactory testing may be a useful diagnostic aid for parkinson disease, but the types of odors most commonly affected need to be identified.	2003	12707068
analysis of the relationship between the structure and aromatic properties of chemical compounds.	this paper presents the results of research on the relationship between the structure and odour properties of a selection of chemical compounds. the research concerns five groups of esters, each with a different smell: almond, apricot, apple, pineapple and rose. the supposed relationship between the smell and certain selected attributes of each molecule was examined by various pattern recognition techniques using programs developed in the department of computer chemistry at rzeszów university of ...	2003	12677351
nuclear magnetic resonance studies on the pka values and interaction of ionizable groups in bromelain inhibitor vi from pineapple stem.	bromelain inhibitor vi (bi-vi), a cysteine proteinase inhibitor from pineapple stem, is a unique double-chain molecule composed of two distinct domains a and b. in order to clarify the molecular mechanism of the proteinase-inhibitor interaction, we investigated the electrostatic properties of this inhibitor. the inhibitory activity toward bromelain was revealed to be maximal at ph 3-4 and the gross conformation to be stable over a wide range of ph. based on these results, ph titration experiment ...	2003	12674503
molecular characterization and allergenic activity of lyc e 2 (beta-fructofuranosidase), a glycosylated allergen of tomato.	until now, only a small amount of information is available about tomato allergens. in the present study, a glycosylated allergen of tomato (lycopersicon esculentum), lyc e 2, was purified from tomato extract by a two-step fplc method. the cdna of two different isoforms of the protein, lyc e 2.01 and lyc e 2.02, was cloned into the bacterial expression vector pet100d. the recombinant proteins were purified by electroelution and refolded. the ige reactivity of both the recombinant and the natural ...	2003	12631291
cocoa-based media for culturing phytophthora palmivora (butl.) butl., causal agent of black pod disease of cocoa.	green cocoa pod husk agar (gcpa), ripe cocoa pod husk agar (rcpa), green cocoa bean agar (gcba), ripe cocoa bean agar (rcba), green cocoa mucilage agar (gcma) and ripe cocoa mucilage agar (rcma) were prepared and assessd for their clarity and for potential to support mycelial growth and sporulation of p. palmivora. oatmeal agar (oma), potato-dextrose agar (pda), vegetable 8 juice agar (v8ja) and pineapple crown agar (pca) were included for comparison. the highest radial growth rates of 8.3 and 7 ...	2002	12617500
[spherical phenyl-regenerated cellulose stationary phases used for hydrophobic chromatography to extract bramelain from crude stem bramelain].	the low-pressure preparative hydrophobic chromatography packing materials have been successfully synthesized by grafting hydrophobic phenyl group to the macro-pore spherical regenerated cellulose resin (diameter 0.7-1.3 mm, pore size 4.3-15.7 microns, specific surface area 88 m2/g). the optimum adsorption-desorption conditions have been got by using 10 ml of this hydrophobic resin to deal with 100 ml of the crude stem bramelain: the mobile phase ph is around 5.0, concentration of (nh4)2so4 is 1. ...	1999	12549169
orbital reconstruction after exenteration: use of a transorbital temporal muscle flap.	orbital exenteration is a disfiguring operation that involves the total removal of the orbital contents with partial or total excision of the eyelids. common methods of orbit reconstruction include pectoralis musculocutaneous pedicled flap and free tissue transfer. the purpose of this study is to illustrate that the entire temporalis muscle may be used by creating a large window in the lateral orbit, without resection of the lateral orbital rim. orbital exenteration was performed on four cadaver ...	2003	12545107
[rapid determination of ethephon residues in concentrated pineapple juice by head-space gas chromatography].	the method developed is based on the special property that ethephon can be easily decomposed into ethene in alkaline solution by heating. certain amount of concentrated koh solution was added to the sample and heated at 70 degrees c. then, 1 ml of the gas above the sample was injected into a gas chromatograph by head-space sampler for the determination of the target compound with external standard quantitation method. the detection limit was 0.025 mg/kg and the fortified recoveries of ethephon i ...	2001	12541822
selection for aluminium tolerance in embryogenic lines of citrus sinensis l. and c. aurantium l.	the citrus cultivars 'amargo' and 'pineapple' produce embryogenic cultures on two types of sugars, galactose and lactose. two percent lactose is optimal for both cultivars; embryogenic callus is produced 8-10 weeks after inoculation. whereas 2% galactose is optimal for 'amargo', it is 3% for 'pineapple'. three types of embryos are recognized: whitish, greenish and heart shaped. in 8 to 10 week old cultures we do also observe a black spotted type of embryos. a high percentage of plant conversion ...	2002	12491964
preliminary comparison of bromelain and ibuprofen for delayed onset muscle soreness management.	the purpose of this study was to determine whether a common bromelain regimen or common ibuprofen regimen are effective in resolving pain and muscle dysfunction associated with delayed onset muscle soreness of the elbow flexors.	2002	12466693
antioxidant and antiproliferative activities of common fruits.	consumption of fruits and vegetables has been associated with reduced risk of chronic diseases such as cardiovascular disease and cancer. phytochemicals, especially phenolics, in fruits and vegetables are suggested to be the major bioactive compounds for the health benefits. however, the phenolic contents and their antioxidant activities in fruits and vegetables were underestimated in the literature, because bound phenolics were not included. this study was designed to investigate the profiles o ...	2002	12452674
structure-function relationship of bromelain isoinhibitors from pineapple stem.	bromelain isoinhibitors from pineapple stem (bis) are unique double-chain inhibitors and inhibit the cysteine proteinase bromelain competitively. the three-dimensional structure was shown to be composed of two distinct domains, each of which is formed by a three-stranded anti-parallel beta-sheet. unexpectedly, bis were found to share similar folding and disulfide-bond connectivities not with the cystatin superfamily, but with bowman-birk trypsin/chymotrypsin inhibitor (bbi). the structural simil ...	2005	12437100
movement of bromacil and hexazinone in soils of hawaiian pineapple fields.	bromacil and hexazinone have been heavily used to control weeds in the pineapple fields in central oahu, hawaii, usa, since 1970s and 1980s, respectively. the last application prior to this study was at a rate of 0.6 kg active ingredient (a.i.) ha(-1) for hexazinone and 1.8 or 3.4 kg (a.i.) ha(-1) for bromacil in the six study fields during june-october 1998. soils were collected from 0 to 1860 cm below the surface in january-may 1999 to survey the residue profiles of the two herbicides. stratop ...	2002	12430654
comparison of the nutrient content of fresh fruit juices vs commercial fruit juices.	to compare the types and quantities of carbohydrate, electrolytes, ph and osmolarity of fresh fruit juices and commercial fruit juices.	2002	12403253
diversifying crops: the nicaraguan experiment.	over 1/2 of nicaragua's population lives in the pacific plains where cotton has been grown intensively for 40 years. this single-crop economy has led to massive deforestation, wind and water erosion has affected the soil, and extensive use of pesticides has deposited excessive amounts of ddt in the breast milk of nursing mothers. after the downfall of the somoza dictatorship the subsequent agrarian reform has been hampered by lack of information and training on sustainable methods of farming. th ...	1992	12317702
natural family planning works in a bukidnon town.	natural family planning is being actively promoted in a pineapple plantation of the philippine packing corporation (ppc) located in northern bukidnon province. prospective acceptors attend 4 seminars which cover sex education and instruction on natural family planning methods. the goal of the program is family life and marriage enrichment rather than family size limitation. thus, there is no target number of acceptors. early acceptors used the basal body temperature method, but this was subseque ...	1983	12265792
pollination increases gibberellin levels in developing ovaries of seeded varieties of citrus.	reproductive and vegetative tissues of the seeded pineapple cultivars of sweet orange (citrus sinensis l.) contained the following c-13 hydroxylated gibberellins (gas): ga53, ga17, ga19, ga20, ga1, ga29, and ga8, as well as ga97, 3-epi-ga1, and several uncharacterized gas. the inclusion of 3-epi-ga1 as an endogenous substance was based on measurements of the isomerization rates of previously added [2h2]ga1. pollination enhanced amounts of ga19, ga20, ga29, and ga8 in developing ovaries. levels o ...	1997	12223728
purification and structural and kinetic characterization of the pyrophosphate:fructose-6-phosphate 1-phosphotransferase from the crassulacean acid metabolism plant, pineapple.	pyrphosphate-dependent phosphofructokinase (pfp) was purified to electrophoretic homogeneity from illuminated pineapple (ananas comosus) leaves. the purified enzyme consists of a single subunit of 61.5 kd that is immunologically related to the potato tuber pfp [beta] subunit. the native form of pfp likely consists of a homodimer of 97.2 kd, as determined by gel filtration. pfp's glycolytic activity was strongly dependent on ph, displaying a maximum at ph 7.7 to 7.9. gluconeogenic activity was re ...	1997	12223643
3d modeling and virtual endoscopy of the small bowel based on magnetic resonance imaging in patients with inflammatory bowel disease.	small bowel mri (mr imaging) is a new imaging modality that excellently depicts small intestine pathology in patients with inflammatory bowel disease. virtual endoscopy based on sectional imaging is a recently introduced technique to create endoscopy like views. the aim of this study was to evaluate the feasibility of virtual small bowel endoscopy based on mr imaging in patients with crohn disease.	2002	12218449
opalescent and cloudy fruit juices: formation and particle stability.	cloudy fruit juices, particularly from tropical fruit, are becoming a fast-growing part of the fruit juice sector. the classification of cloud as coarse and fine clouds by centrifugation and composition of cloud from apple, pineapple, orange, guava, and lemon juice are described. fine particulate is shown to be the true stable cloud and to contain considerable protein, carbohydrate, and lipid components. often, tannin is present as well. the fine cloud probably arises from cell membranes and app ...	2002	12180775
swaziland.	swaziland is a country of 17,363 sq km with 860,000 inhabitants, of whom 64% are literate. independence was gained on september 6, 1968. the terrain consists of mountains and plateaus, with a climate variously near-temperature, subtropical, and semi-arid. english and siswati are spoken by swazi, zulu, and non-african ethnic groups, who variously hold christian and indigenous beliefs. life expectancy ranges between 53 and 60 years, gdp is $704 million, growing at a rate of 7%. per capita in ...	1993	12178057
asean.	asean is a group of counties encompassing 3 million sq. km with 333 million inhabitants, of whom approximately 65% are literate. the group was formed august 8, 1967, and includes brunei, indonesia, malaysia, the philippines, singapore, and thailand. malay, indonesian, filipino, thai, chinese, and english are spoken by malay, thai, chinese, indian, and other ethnic groups who variously are of the islamic, buddhist, christian, taoist, and hindu faiths. life expectancy is approximately 85 years, wi ...	1992	12178041
new sources of dietary myosmine uptake from cereals, fruits, vegetables, and milk.	myosmine has been regarded as a specific tobacco alkaloid until investigations pointed out that nuts and nut products constitute a significant source of myosmine. in the present study it is shown that the occurrence of myosmine is widespread throughout a large number of plant families. using a method for extraction practicable for all examined foods, quantitative analysis through internal standard addition showed nanograms per gram amounts. positively tested edibles were staple foods such as mai ...	2002	12166981
bromelain treatment alters leukocyte expression of cell surface molecules involved in cellular adhesion and activation.	bromelain is a natural proteinase preparation derived from pineapple stem that is marketed for oral use as a digestive aid and as an antiinflammatory agent. bromelain treatment in vitro has been previously shown to selectively remove certain cell surface molecules that may affect lymphocyte migration and activation. this study reports the effects of bromelain on a broad range of cell surface molecules and on lymphocytes, monocytes, and granulocytes under physiologically relevant conditions. in v ...	2002	12165279
identification and occurrence of the novel alkaloid pentahydroxypentyl-tetrahydro-beta-carboline-3-carboxylic acid as a tryptophan glycoconjugate in fruit juices and jams.	the novel carbohydrate-derived beta-carboline, 1-pentahydroxypentyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid, was identified in fruit- and vegetable-derived products such as juices, jams, and tomato sauces. this compound occurred as two diastereoisomers, a cis isomer (the major compound) and a trans isomer, ranging from undetectable amounts to 6.5 microg/g. grape, tomato, pineapple, and tropical juices exhibited the highest amount of this alkaloid (up to 3.8 mg/l), whereas apple, bana ...	2002	12137498
ige reactivity to profilin in pollen-sensitized subjects with adverse reactions to banana and pineapple.	the so-called 'latex-fruit syndrome' is a well-documented phenomenon in cross-reactive allergies. by contrast, there is a lack of information about allergy to exotic fruits in patients with a predominant pollen sensitization. since the ubiquitous protein profilin has been identified as an allergen in natural rubber latex as well as in pollen-related foods, the aim of this study was to investigate the role of profilin in allergy to certain exotic fruits.	2002	12065910
characterization of genomic sequence coding for bromelain inhibitors in pineapple and expression of its recombinant isoform.	bromelain inhibitor (bi) is a cysteine proteinase inhibitor isolated from pineapple stem (reddy, m. n., keim, p. s., heinrikson, r. l., and kézdy, f. j. (1975) j. biol. chem. 250, 1741-1750). it consists of eight isoinhibitors, and each isoinhibitor has a two-chain structure. in this study, the genomic dna has been cloned and found to encode a precursor protein with 246 amino acids (m(r) = approximately 27,500) containing three isoinhibitor domains (bi-iii, -vi, and -vii) that are 93% identical ...	2002	12016215
total antioxidant and ascorbic acid content of fresh fruits and vegetables: implications for dietary planning and food preservation.	epidemiological evidence links high intake of ascorbic acid (aa) and other antioxidant micronutrients to health promotion. it would be useful to know the overall, or 'total' antioxidant capacity of foods, to establish the contribution of aa to this, and to assess how this information may translate into dietary intakes to meet the new us daily reference intake for aa. in this study, the total antioxidant capacity, as the ferric reducing-antioxidant power (frap) value, and aa content of thirty-fou ...	2002	11898770
hydrogen isotopic profile in the characterization of sugars. influence of the metabolic pathway.	the site-specific natural hydrogen isotope ratios of plant metabolites determined by 2h nuclear magnetic resonance (snif-nmr method) can provide powerful criteria for inferring mechanistic and environmental effects on biosynthetic pathways. this work examines the potential of isotopic profiles for the main constituents of carbohydrates, glucose and fructose, to distinguish different photosynthetic pathways. an appropriate analytical strategy, involving three suitable isotopic probes, has been el ...	2002	11879039
high-performance liquid chromatographic analysis of free amino acids in fruit juices using derivatization with 9-fluorenylmethyl-chloroformate.	a simple, rapid, and reliable reversed-phase high-performance liquid chromatographic method for the analysis of 16 amino acids of main interest in commercial fruit juices (pear, orange, grapefruit, pineapple, peach, and apricot) is described. no sample cleanup is required. the ph of the fruit juices is adjusted to alkaline value (8.5) using 200 mm borate buffer, then amino acid is converted to stable derivatives using 9-fluorenylmethyl-chloroformate. the excess of derivatization reagent is remov ...	2002	11866381
a comparative study on diurnal changes in metabolite levels in the leaves of three crassulacean acid metabolism (cam) species, ananas comosus, kalanchoë daigremontiana and k. pinnata.	a comparative study on diurnal changes in metabolite levels associated with crassulacean acid metabolism (cam) in the leaves of three cam species, ananas comosus (pineapple), a hexose-utilizing species, and kalanchoë daigremontiana and k. pinnata, two starch-utilizing species, were made. all three cam species showed a typical feature of cam with nocturnal malate increase. in the two kalanchoë species, isocitrate levels were higher than citrate levels; the reverse was the case in pineapple. in th ...	2002	11807138
bromelain reversibly inhibits invasive properties of glioma cells.	bromelain is an aqueous extract from pineapple stem that contains proteinases and exhibits pleiotropic therapeutic effects, i.e., antiedematous, antiinflammatory, antimetastatic, antithrombotic, and fibrinolytic activities. in this study, we tested bromelain's effects on glioma cells to assess whether bromelain could be a potential contributor to new antiinvasive strategies for gliomas. several complementary assays demonstrated that bromelain significantly and reversibly reduced glioma cell adhe ...	2001	11774029
evaluation of the antioxidant properties of mediterranean and tropical fruits compared with common food additives.	several mediterranean and tropical fruits have been analyzed in order to assess their antioxidant activity compared with that of common food additives (butylated hydroxyanisole [bha], butylated hydroxytoluene [bht] and propyl gallate). among mediterranean fruits, red grape and plum were more effective (p < 0.05) scavengers of peroxyl radicals than bha, bht, and propyl gallate. of the tropical fruits, banana was the most effective scavenger of peroxyl radicals. mediterranean and tropical fruits s ...	2001	11770635
small bowel review: normal physiology part 1.	in the past year there have been many advances in the area of small bowel physiology and pathology and therapy. in preparation for this review, over 1500 papers were assessed. the focus is on presenting clinically useful information for the practising gastroenterologist. selected important clinical learning points include the following: (1) glucose absorption mediated by sglt1 is controlled by mrna abundance, as well as by posttranscriptional processes including protein trafficking; (2) inducers ...	2001	11768247
proteolytic inhibition of salmonella enterica serovar typhimurium-induced activation of the mitogen-activated protein kinases erk and jnk in cultured human intestinal cells.	bromelain, a mixture of cysteine proteases from pineapple stems, blocks signaling by the mitogen-activated protein (map) kinases extracellular regulated kinase 1 (erk-1) and erk-2, inhibits inflammation, and protects against enterotoxigenic escherichia coli infection. in this study, we examined the effect of bromelain on salmonella enterica serovar typhimurium infection, since an important feature of its pathogenesis is its ability to induce activation of erk-1 and erk-2, which leads to internal ...	2002	11748167
antibody binding to venom carbohydrates is a frequent cause for double positivity to honeybee and yellow jacket venom in patients with stinging-insect allergy.	up to 50% of patients with stinging-insect allergy have double-positive rast results to honeybee and yellow jacket (yj) venom. true double sensitization and crossreactivity through venom hyaluronidases are considered main reasons for this multiple reactivity.	2001	11742287
characterization of yeast strains for wine production: effect of fermentation variables on quality of wine produced.	sixteen yeast strains isolated from grapefruit (citrus paradis), orange (citrus sinensis) and pineapple (ananas comosus) were characterized using standard microbiological procedures. the species were identified as saccharomyces uvarum, s. cerevisiae, s. carlbergensis, and s. ellipsoideus. their abilities for wine production were tested by using sugar and ethanol tolerance tests. the best biochemically active strain, s. ellipsoideus, was used along with commercially available baker's yeast (s. ce ...	2001	11732717
water quality of a reservoir as affected by agriculture in the east of thailand: a preliminary study.	a preliminary study on the water quality of a reservoir, affected by agriculture, in the east of thailand was conducted during 1996-1997. monitoring water quality of a reservoir is important because the sloping lands surrounding the reservoirs are mainly utilized for cultivating cash crops (pineapple, cassava, etc). a lot of fertilizers and agrochemicals were applied to soil and crops which can polluted the water. the results from the preliminary studied will be applied for the monitoring of the ...	2001	11724479