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suspension cell culture as a tool for the characterization of class iii peroxidases in sugarcane.secreted class iii peroxidases (ec 1.11.1.7) are implicated in a broad range of physiological processes throughout the plant life cycle. however, the unambiguous determination of the precise biological role of an individual class iii peroxidase isoenzyme is still a difficult task due to genetic redundancy and broad substrate specificity in vitro. in addition, many difficulties are encountered during extraction and analysis of cell wall proteins. since class iii peroxidases are also secreted into ...201323159486
evaluation of corn hybrids expressing cry1f, cry1a.105, cry2ab2, cry34ab1/cry35ab1, and cry3bb1 against southern united states insect pests.studies were conducted across the southern united states to characterize the efficacy of multiple bacillus thuringiensis (bt) events in a field corn, zea mays l., hybrid for control of common lepidopteran and coleopteran pests. cry1f protein in event tc1507 and cry1a.105 + cry2ab2 proteins in event mon 89034 were evaluated against pests infesting corn on above-ground plant tissue including foliage, stalks, and ears. cry34ab1/cry35ab1 proteins in event das-59122-7 and cry3bb1 in event mon 88017 w ...201223156183
short-term usage of sewage sludge as organic fertilizer to sugarcane in a tropical soil bears little threat of heavy metal contamination.a field experiment was carried out to study the effect of application rates of sewage sludge and mineral nitrogen and phosphate fertilizers on as, ba, cd, cr, cu, ni, pb, se, and zn concentration in soil, cane plant, and first ratoon (residual effect) in a typic hapludult soil. to allow an analysis by means of response surface modeling, four rates of sewage sludge (0, 3.6, 7.2 and 10.8 t ha(-1), dry base), of n (0, 30, 60 and 90 kg ha(-1)) and of p(2)o(5) (0, 60, 120 and 180 kg ha(-1)) were appl ...201323137916
effects of drought on the microtranscriptome of field-grown sugarcane plants.sugarcane (saccharum spp.) is the most promising crop for renewable energy. among the diverse stresses that affect plant productivity, drought stress frequently causes losses in sugarcane fields. although several studies have addressed plant responses to drought using controlled environments, plant responses under field conditions are largely unknown. recently, microrna (mirna)-mediated post-transcriptional regulation has been described as an important and decisive component in vegetal developme ...201323129215
butanol production in a first-generation brazilian sugarcane biorefinery: technical aspects and economics of greenfield projects.the techno-economics of greenfield projects of a first-generation sugarcane biorefinery aimed to produce ethanol, sugar, power, and n-butanol was conducted taking into account different butanol fermentation technologies (regular microorganism and mutant strain with improved butanol yield) and market scenarios (chemicals and automotive fuel). the complete sugarcane biorefinery with the batch acetone-butanol-ethanol (abe) fermentation process was simulated using aspen plus®. the biorefinery was de ...201323127845
genetic diversity and population structure of sugarcane mosaic virus.sugarcane mosaic virus (scmv) is one of the causal pathogens of mosaic diseases on sugarcane, maize, sorghum and some other graminaceous species with worldwide distribution. the global genetic diversity and molecular evolution of scmv capsid protein (cp) gene were investigated with the nucleotide sequences available in the genbank database. phylogenetic analyses revealed that scmv isolates clustered in relation to their original hosts, and geographically distinct isolates from maize or sugarcane ...201323116592
rna interference of endochitinases in the sugarcane endophyte trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease.the sugarcane root endophyte trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including n-acetyl-ß-d-glucosaminidases, chitobiosidases and endochitinases. we used targeted gene deletion and rna-dependent gene silencing strategies to disrupt n-acetyl-ß-d-glucosaminidase and endochitinase activities of the ...201223110120
hantavirus pulmonary syndrome in santa cruz, bolivia: outbreak investigation and antibody prevalence study.we report the results of an investigation of a small outbreak of hantavirus pulmonary syndrome in 2002 in the department of santa cruz, bolivia, where the disease had not previously been reported. two cases were initially reported. the first case was a physician infected with laguna negra virus during a weekend visit to his ranch. four other persons living on the ranch were igm antibody-positive, two of whom were symptomatic for mild hantavirus pulmonary syndrome. the second case was a migrant s ...201223094116
in vitro generation of somaclonal variant plants of sugarcane for tolerance to fusarium sacchari.key message : a combination of in vitro culture and mutagenesis using ethyl methanesulfonate (ems) followed by culture filtrate-mediated selection produced variant sugarcane plants tolerant and resistant to fusarium sacchari. eldana saccharina is a destructive pest of the sugarcane crop in south africa. fusarium sacchari png40 (a fungal strain harmful to e. saccharina) has the potential to be an endophytic biological control agent of the stalk borer. however, the fungus causes fusarium stalk rot ...201323090725
unintended consequences of bioethanol feedstock choice in china.economic, energy, and environmental impacts of 11 types of bioethanol feedstock in china were evaluated using a mixed-unit input-output life cycle assessment model. corn grain and wheat grain had higher negative economic, energy, and environmental impacts. sweet sorghum, cassava, sugar beet, and sugarcane showed better economic performance but increasing negative energy and environmental impacts. cellulose-based feedstocks in general showed positive economic, energy, and environmental performanc ...201223070140
functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum.transposable elements (tes) account for a large portion of plant genomes, particularly in grasses, in which they correspond to 50%-80% of the genomic content. tes have recently been shown to be a source of new genes and new regulatory networks. the most striking contribution of tes is referred as "molecular domestication", by which the element coding sequence loses its movement capacity and acquires cellular function. recently, domesticated transposases known as mustang and derived from the muta ...201223055803
expression and characterization of a gh39 β-xylosidase ii from caulobacter crescentus.in the present work, the gene xynb2, encoding a β-xylosidase ii of the glycoside hydrolase 39 (gh39) family, of caulobacter crescentus was cloned and successfully overexpressed in escherichia coli dh10b. the recombinant protein (ccxynb2) was purified using nickel-sepharose affinity chromatography, with a recovery yield of 75.5 %. ccxynb2 appeared as a single band of 60 kda on a sodium dodecyl sulfate polyacrylamide gel and was recognized by a specific polyclonal antiserum. the predicted ccxynb2 ...201223054825
an alkaline thermostable recombinant humicola grisea var. thermoidea cellobiohydrolase presents bifunctional (endo/exoglucanase) activity on cellulosic substrates.humicola grisea var. thermoidea is a deuteromycete which secretes a large spectrum of hydrolytic enzymes when grown on lignocellulosic residues. this study focused on the heterologous expression and recombinant enzyme analysis of the major secreted cellulase when the fungus is grown on sugarcane bagasse as the sole carbon source. cellobiohydrolase 1.2 (cbh 1.2) cdna was cloned in pichia pastoris under control of the aox1 promoter. recombinant protein (rcbh1.2) was efficiently produced and secret ...201323054694
whole-genome sequence of enterobacter sp. strain sst3, an endophyte isolated from jamaican sugarcane (saccharum sp.) stalk tissue.enterobacter sp. strain sst3 is an endophytic bacterium isolated from saccharum spp. here we present its annotated draft genome that may shed light on its role as a bacterial endophyte of sugarcane. to our knowledge, this is the first genome announcement of a sugarcane-associated bacterium from the genus enterobacter.201223045495
high production of cellulose degrading endo-1,4-β-d-glucanase using bagasse as a substrate from bacillus subtilis kibge has.sugarcane bagasse is a cheap carbon source for endo-1,4-β-d-glucanase production as it is easily available as by-product from sugar industries. fermentation conditions for endo-1,4-β-d-glucanase production by bacillus subtilis kibge has were optimized by using un-treated sugarcane bagasse for induction of endo-1,4-β-d-glucanase and it was found that 2.0 g% bagasse in fermentation medium induced maximum endo-1,4-β-d-glucanase production. it was also found that when sugarcane bagasse was supplemen ...201323044136
recovery of purified lactonic sophorolipids by spontaneous crystallization during the fermentation of sugarcane molasses with candida albicans o-13-1.numerous studies have focused on how to obtain high yield of sophorolipids using low-cost materials as substrates, and there has been various work on the experimental methods for purifying lactonic sophorolipids. these studies have not yet obtained satisfied results in combining a low-cost fermentation process and the purification of lactonic sophorolipids. this study establishes a fed-batch fermentation process of purifying sophorolipids from candida albicans o-13-1 using low-cost sugarcane mol ...201223040390
a designed bifunctional laccase/β-1,3-1,4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse.a bifunctional enzyme has been created by fusing two bacillus subtilis enzymes: the β-1,3-1,4-glucanase (bgls, ec 3.2.1.73) that hydrolyzes plant cell wall β-glucans and the copper-dependent oxidase laccase (cota, ec 1.10.3.2) that catalyzes the oxidation of aromatic compounds with simultaneous reduction of oxygen to water. the chimeric laccase/β-1,3-1,4-glucanase was created by insertion fusion of the bgls and cota genes, and expressed in escherichia coli. the affinity-purified recombinant chim ...201323012443
molecular characterization of indian sugarcane streak mosaic virus isolate.sugarcane streak mosaic virus (scsmv), a member of the genus poacevirus, family potyviridae, is an important viral pathogen affecting sugarcane cultivation in india. the complete nucleotide sequence of a scsmv isolate from india (scsmv-ind) was determined. the linear, assembled, single-stranded positive-sense rna genome of scsmv-ind was 9,786 nucleotides in length (excluding the poly (a) tail) and encoded a polyprotein of 3,131 amino acid residues. the genome of scsmv-ind shared high degree of s ...201323011777
a review on soil carbon accumulation due to the management change of major brazilian agricultural activities.agricultural areas deal with enormous co2 intake fluxes offering an opportunity for greenhouse effect mitigation. in this work we studied the potential of soil carbon sequestration due to the management conversion in major agricultural activities in brazil. data from several studies indicate that in soybean/maize, and related rotation systems, a significant soil carbon sequestration was observed over the year of conversion from conventional to no-till practices, with a mean rate of 0.41 mg c ha( ...201223011303
succinate production by metabolically engineered escherichia coli using sugarcane bagasse hydrolysate as the carbon source.efficient biosynthesis of succinate from a renewable biomass resource by engineered escherichia coli is reported in this paper. fermentation of sugarcane bagasse hydrolysate by engineered e. coli ba204, a pflb, ldha, and ppc deletion strain overexpressing the atp-forming phosphoenolpyruvate carboxykinase from bacillus subtilis 168, produced a final succinate concentration of 15.85 g l(-1) with a high yield of 0.89 g l(-1) total sugar under anaerobic conditions. during dual-phase fermentations, i ...201323010211
beauveria brongniartii on white grubs attacking sugarcane in south africa.beauveria brongniartii (saccardo) petch fungal infections were observed on the melolonthid hypopholis sommeri burmeister (coleoptera: scarabaeidae) at two sites (harden heights and canema) in the sugarcane producing area of the northern kwazulu-natal midlands of south africa. to initially identify the disease-causing fungus, 17 different fluorescently-labelled microsatellite pcr primers were used to target 78 isolates of beauveria spp. dna. microsatellite data resolved two distinct clusters of b ...201222982079
using population genetic methods to identify the origin of an invasive population and to diagnose cryptic subspecies of telchin licus (lepidoptera: castniidae).telchin licus, the giant sugarcane borer, is an important pest species of sugarcane in northeast brazil. four subspecies of telchin licus are recognized in brazil based on their geographic distribution and subtle differences in wing colour pattern. some taxa are morphologically indistinguishable, and their accurate identification is key to their efficient control. mitochondrial genes sequences (cytochrome oxidase i and subunit 6 of the nicotinamide adenine dinucleotide dehydrogenase) were applie ...201322971459
utilization of sugarcane habitat by feral pig (sus scrofa) in northern tropical queensland: evidence from the stable isotope composition of hair.feral pigs (sus scrofa) are an invasive species that disrupt ecosystem functioning throughout their introduced range. in tropical environments, feral pigs are associated with predation and displacement of endangered species, modification of habitat, and act as a vector for the spread of exotic vegetation and disease. across many parts of their introduced range, the diet of feral pigs is poorly known. although the remote location and difficult terrain of far north queensland makes observing feral ...201222957029
moisture source and diet affect development and reproduction of orius thripoborus and orius naivashae, two predatory anthocorids from southern africa.the effect of moisture source and diet on the development and reproduction of the pirate bugs, orius thripoborus (hesse) and orius naivashae (poppius) (hemiptera: anthocoridae) was examined in the laboratory. both species had been collected in and around sugarcane fields in south africa. supplementing eggs of the flour moth ephestia kuehniella (zeller) (lepidoptera: pyralidae) with a green bean pod as a moisture source yielded better nymphal survival and faster development, as compared with free ...201222935002
fungal rock phosphate solubilization using sugarcane bagasse.the effects of different doses of rock phosphate (rp), sucrose, and (nh(4))(2)so(4) on the solubilization of rp from araxá and catalão (brazil) by aspergillus niger, penicillium canescens, eupenicillium ludwigii, and penicillium islandicum were evaluated in a solid-state fermentation (ssf) system with sugarcane bagasse. the factors evaluated were combined following a 2(3) + 1 factorial design to determine their optimum concentrations. the fitted response surfaces showed that higher doses of rp p ...201322927013
diversity and physiological characterization of d-xylose-fermenting yeasts isolated from the brazilian amazonian forest.this study is the first to investigate the brazilian amazonian forest to identify new d-xylose-fermenting yeasts that might potentially be used in the production of ethanol from sugarcane bagasse hemicellulosic hydrolysates.201222912807
reuteran and levan as carbohydrate sinks in transgenic sugarcane.the present study reports the effect of high molecular weight bacterial fructan (levan) and glucan (reuteran) on growth and carbohydrate partitioning in transgenic sugarcane plants. these biopolymers are products of bacterial glycosyltransferases, enzymes that catalyze the polymerization of glucose or fructose residues from sucrose. constructs, targeted to different subcellular compartments (cell wall and cytosol) and driven by the cauliflower mosaic virus-35s: maize-ubiquitin promoter, were int ...201222903192
genetic characterization and construction of an auxotrophic strain of saccharomyces cerevisiae jp1, a brazilian industrial yeast strain for bioethanol production.used for millennia to produce beverages and food, saccharomyces cerevisiae also became a workhorse in the production of biofuels, most notably bioethanol. yeast strains have acquired distinct characteristics that are the result of evolutionary adaptation to the stresses of industrial ethanol production. jp1 is a dominant industrial s. cerevisiae strain isolated from a sugarcane mill and is becoming increasingly popular for bioethanol production in brazil. in this work, we carried out the genetic ...201222892884
tissue-specific expression and functional role of dehydrins in heat tolerance of sugarcane (saccharum officinarum).studies on the functional roles of dehydrins (dhns) in heat tolerance of plants are scarce. this study was conducted to immunohistolocalize dhns in leaves of heat-tolerant (cp-4333) and heat-sensitive (hsf-240) sugarcane (saccharum officinarum l.) clones at three phenological stages in order to elucidate their putative roles under heat stress. cp-4333 indicated greater amounts of heat-stable proteins than hsf-240 under heat stress. western blotting revealed the expression of three dhns in cp-433 ...201322886313
acetobacter okinawensis sp. nov., acetobacter papayae sp. nov., and acetobacter persicus sp. nov.; novel acetic acid bacteria isolated from stems of sugarcane, fruits, and a flower in japan.eleven strains of acetic acid bacteria were isolated from stems of sugarcane, fruits, and a flower in japan. the isolates were separated into three groups, groups i, ii, and iii, in the genus acetobacter according to phylogenetic analysis based on 16s rrna sequences. the isolates had sequence similarities of 99.8-100% within the group, 99.3-99.6% to those of the type strains of each related acetobacter species, and less than 98.4% to those of the type strains of other acetobacter species. genomi ...201222878741
sugarcane growth promotion by the endophytic bacterium pantoea agglomerans 33.1.the promotion of sugarcane growth by the endophytic pantoea agglomerans strain 33.1 was studied under gnotobiotic and greenhouse conditions. the green fluorescent protein (gfp)-tagged strain p. agglomerans 33.1::pnkgfp was monitored in vitro in sugarcane plants by microscopy, reisolation, and quantitative pcr (qpcr). using qpcr and reisolation 4 and 15 days after inoculation, we observed that gfp-tagged strains reached similar density levels both in the rhizosphere and inside the roots and aeria ...201222865062
acid black 48 dye biosorption using saccharomyces cerevisiae immobilized with treated sugarcane bagasse.the textile industry consumes large quantities of water and chemicals, especially in dyeing and finishing processes. textile dye adsorption can be accomplished with natural or synthetic compounds. cell immobilization using biomaterials allows the reduction of toxicity and mechanical resistance and opens spaces within the matrix for cell growth. the use of natural materials, such as sugarcane bagasse, is promising due to the low costs involved. the aim of the present study was to evaluate the use ...201222864427
the consequences of lactobacillus vini and dekkera bruxellensis as contaminants of the sugarcane-based ethanol fermentation.this work describes the effects of the presence of the yeast dekkera bruxellensis and the bacterium lactobacillus vini on the industrial production of ethanol from sugarcane fermentation. both contaminants were quantified in industrial samples, and their presence was correlated to a decrease in ethanol concentration and accumulation of sugar. then, laboratory mixed-cell fermentations were carried out to evaluate the effects of these presumed contaminants on the viability of saccharomyces cerevis ...201222842986
first report of trypanosoma vivax outbreak in dairy cattle in são paulo state, brazil.this is the first description of a trypanosoma vivax outbreak in the state of são paulo (municipality of lins). fever, jaundice, decreased milk production, weight loss, profuse diarrhea, abortion, anemia, leukocytosis and hyperfibrinogenemia were observed in the affected animals. thirty-one cows and calves died out of a total of 1080 in the herd. three cows showed neurological symptoms like dysmetria, ataxia, muscle weakness, ptyalism, lymph node enlargement and submandibular edema. flagellated ...201322832751
high gravity fermentation of sugarcane molasses to produce ethanol: effect of nutrients.fermentation efficiency of more than 85% was obtained by high gravity fermentation of 33-34°bx (spec. gravity ≈1.134) molasses medium with certain nutrients, instead of generally employed medium containing ≈16% (w/v) total sugar (spec. gravity ≈1.090) for ethanol fermentation in distilleries to get maximum 80-85% conversion. the fermenting yeast, saccharomyces, has varied capabilities, depending on the species and nutrition for fermenting the high solids medium. the fermentation period was reduc ...201022815577
identification and validation of reference genes to study the gene expression in gluconacetobacter diazotrophicus grown in different carbon sources using rt-qpcr.gluconacetobacter diazotrophicus strain pal5 is a nitrogen-fixing endophytic bacterium originally isolated from sugarcane and later on was found to colonize other plants such as rice, elephant grass, sweet potato, coffee, and pineapple. currently, g. diazotrophicus has been considered a plant growth-promoting bacterium due to its characteristics of biological nitrogen fixation, phytohormone secretion, solubilization of mineral nutrients and antagonism to phytopathogens. reverse transcription fol ...201222814372
concentrations of cu, fe, mn, and zn in tropical soils amended with sewage sludge and composted sewage sludge.sewage sludge may be used as an agricultural fertilizer, but the practice has been criticized because sludge may contain trace elements and pathogens. the aim of this study was to compare the effectiveness of total and pseudototal extractants of cu, fe, mn, and zn, and to compare the results with the bioavailable concentrations of these elements to maize and sugarcane in a soil that was amended with sewage sludge for 13 consecutive years and in a separate soil that was amended a single time with ...201322810380
identification of amino acids important for substrate specificity in sucrose transporters using gene shuffling.plant sucrose transporters (suts) are h(+)-coupled uptake transporters. type i and ii (suts) are phylogenetically related but have different substrate specificities. type i suts transport sucrose, maltose, and a wide range of natural and synthetic α- and β-glucosides. type ii suts are more selective for sucrose and maltose. here, we investigated the structural basis for this difference in substrate specificity. we used a novel gene shuffling method called synthetic template shuffling to introduc ...201222807445
evaluation of in vitro and in vivo effects of semipurified proteinase inhibitors from theobroma seeds on midgut protease activity of lepidopteran pest insects.we have characterized in vitro and in vivo effects of trypsin inhibitors from theobroma seeds on the activity of trypsin- and chymotrypsin-like proteins from lepidopteran pest insects. the action of semipurified trypsin inhibitors from theobroma was evaluated by the inhibition of bovine trypsin and chymotrypsin activities determined by the hydrolysis of n-benzoyl-dl-arginine-p-nitroanilide (bapa) and n-succinyl-ala-ala-pho-phe p-nitroanilide (s-(ala)2prophe-pna). proteinase inhibitor activities ...201222806759
candidates for symbiotic control of sugarcane white leaf disease.the leafhopper matsumuratettix hiroglyphicus (matsumura) is the most important vector of a phytoplasma pathogen causing sugarcane white leaf (scwl) disease. the purpose of this study was to evaluate candidate bacterial symbionts for possible use as vehicles in the control of the disease. 16s rrna bacterial genes were amplified from whole bodies of m. hiroglyphicus leafhoppers and analyzed by cloning and sequencing. two dominant groups were found: one belonged to the betaproteobacteria that did n ...201222798373
metschnikowia saccharicola sp. nov. and metschnikowia lopburiensis sp. nov., two novel yeast species isolated from phylloplane in thailand.five strains representing two novel anamorphic yeast species were isolated from the external surface of sugarcane leaves (dmku-rk16, dmku-rk24, dmku-rk198 and dmku-rk500(t)) and rice leaf (dmku-rk277(t)) by an enrichment technique. on the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the d1/d2 domain of the large subunit (lsu) rrna gene and the internal transcribed spacer region, the five strains were assigned to be two novel spec ...201222791018
study of sugarcane pieces as yeast supports for ethanol production from sugarcane juice and molasses using newly isolated yeast from toddy sap.a repeated batch fermentation system was used to produce ethanol using saccharomyces cerevisiae strain (ncim 3640) immobilized on sugarcane (saccharum officinarum l.) pieces. for comparison free cells were also used to produce ethanol by repeated batch fermentation. scanning electron microscopy evidently showed that cell immobilization resulted in firm adsorption of the yeast cells within subsurface cavities, capillary flow through the vessels of the vascular bundle structure, and attachment of ...201222783132
health impact assessment of liquid biofuel production.bioethanol and biodiesel as potential substitutes for fossil fuels in the transportation sector have been analyzed for environmental suitability. however, there could be impacts on human health during the production, therefore adverse health effects have to be analyzed. the aim of this study is to analyze to what health risk factors humans are exposed to in the production of biofuels and what the size of the health effects is. a health impact assessment expressed as disability adjusted life year ...201322774773
the treatment of jaundice with medicinal plants in indigenous communities of the sub-himalayan region of uttarakhand, india.inspite of tremendous advances made in allopathic medical practices, herbs still play an important role in the management of various liver diseases. a large number of plants and formulations have been claimed to have hepatoprotective activity. jaundice is a symptom, indicative of the malfunctioning of the liver. this paper provides ethnomedicinal information on the plants used to treat jaundice by three important indigenous communities, i.e., nomadic gujjars, tharu and bhoxa of sub-himalayan reg ...201222759701
molecular characterization and phylogenetic analysis of sugarcane yellow leaf virus isolates from china.sugarcane yellow leaf virus (scylv) (genus polerovirus, family luteoviridae), the causal agent of sugarcane yellow leaf disease (yld), was first detected in china in 2006. to assess the distribution of scylv in the major sugarcane-growing chinese provinces, leaf samples from 22 sugarcane clones (saccharum spp. hybrid) showing yld symptoms were collected and analyzed for infection by the virus using reverse transcription pcr (rt-pcr), quantitative rt-pcr, and immunological assays. a complete geno ...201222752564
functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides.plant cell wall degrading enzymes are key technological components in biomass bioconversion platforms for lignocellulosic materials transformation. cost effective production of enzymes and identification of efficient degradation routes are two economic bottlenecks that currently limit the use of renewable feedstocks through an environmental friendly pathway. the present study describes the hypersecretion of an endo-xylanase (gh11) and an arabinofuranosidase (gh54) by a fungal expression system w ...201222750495
blue native-page analysis of trichoderma harzianum secretome reveals cellulases and hemicellulases working as multienzymatic complexes.plant cell wall-degrading enzymes produced by microorganisms possess important biotechnological applications, including biofuel production. some anaerobic bacteria are able to produce multienzymatic complexes called cellulosomes while filamentous fungi normally secrete individual hydrolytic enzymes that act synergistically for polysaccharide degradation. here, we present evidence that the fungus trichoderma harzianum, cultivated in medium containing the agricultural residue sugarcane bagasse, is ...201222744980
halopriming mediated salt and iso-osmotic peg stress tolerance and, gene expression profiling in sugarcane (saccharum officinarum l.).seed priming is a well known pre-germination strategy that improves seed performance. however, biochemical and molecular mechanisms underlying priming mediated stress tolerance are little understood. here, we report results of the study on growth, physiological characteristics and expression of stress responsive genes in salt primed sugarcane cv. co 86032 plants in response to salt (nacl, 150 mm) or iso-osmotic (-0.7 mpa) polyethylene glycol-peg 8000 (20 % w/v) stress exposure for 15 days. varia ...201222740137
oviposition behavior of diatraea saccharalis (lepidoptera: crambidae) on different rice cultivars in louisiana.the sugarcane borer, diatraea saccharalis (f.), is a pest of a variety of graminaceous crops in the southern united states, including sugarcane, maize, and rice in louisiana. this study examined several aspects of d. saccharalis oviposition behavior on rice (oryza sativa). the vertical distribution of egg masses on four phenological stages of rice in the field showed that d. saccharalis prefers to oviposit on the uppermost portions of rice plants, regardless of plant stage. in greenhouse choice ...201222732615
pretreatment of sugarcane bagasse with nh4oh-h2o2 and ionic liquid for efficient hydrolysis and bioethanol production.an efficient pretreatment method using nh(4)oh-h(2)o(2) and ionic liquid (il) was developed for the recovery of cellulose from sugarcane bagasse (scb). the regenerated scb from the combined pretreatment exhibited significantly enhanced enzymatic digestibility with an efficiency of 91.4% after 12h of hydrolysis, which was 64% higher than the efficiency observed for the regenerated scb after the individual nh(4)oh-h(2)o(2) pretreatment. 1-allyl-3-methylimidazolium chloride ([amim]cl) dissolved the ...201222728201
metabolic engineering of klebsiella oxytoca m5a1 to produce optically pure d-lactate in mineral salts medium.klebsiella oxytoca strains were constructed to produce optical pure d-lactate by ph-controlled batch fermentation in mineral salts medium. the alcohol dehydrogenase gene, adhe, and the phospho-transacetylase/acetate kinase a genes, pta-acka, were deleted from the wild type. kms002 (δadhe) and kms004 (δadhe δpta-acka) exhibited d-lactate production as a primary pathway for the regeneration of nad(+). both strains produced 11-13 g/l of d-lactate in medium containing 2% (w/v) glucose with yields of ...201222728200
recombinant expression and biochemical characterization of sugarcane legumain.plant legumains, also termed vacuolar processing enzymes (vpes), are cysteine peptidases that play key roles in plant development, senescence, programmed cell death and defense against pathogens. despite the increasing number of reports on plant cysteine peptidases, including vpes, the characterization of sugarcane vpes and their inhibition by endogenous cystatins have not yet been described. this is the first report of the biochemical characterization of a sugarcane cysteine peptidase. in this ...201222721948
decolorization of a recalcitrant organic compound (melanoidin) by a novel thermotolerant yeast, candida tropicalis rg-9.sugarcane distilleries use molasses for ethanol production and generate large volume of effluent containing high biological oxygen demand (bod) and chemical oxygen demand (cod) along with melanoidin pigment. melanoidin is a recalcitrant compound that causes several toxic effects on living system, therefore, may be treated before disposal. the aim of this study was to isolate a potential thermotolerant melanoidin decolorizing yeast from natural resources, and optimized different physico-chemical ...201222708874
occupational exposure to pesticides and parkinson's disease: a systematic review and meta-analysis of cohort studies.to systematically review available cohort studies and estimate quantitatively the association between occupational exposure to pesticides and parkinson's disease (pd).201222698719
a novel dirigent protein gene with highly stem-specific expression from sugarcane, response to drought, salt and oxidative stresses.dirigent and dirigent-like family proteins contain a number of proteins involved in lignification or in the response to pathogen infection and abiotic stress in plants. in the present study, a full-length cdna sequence of a dirigent-like gene designated scdir (genbank accession number jq622282) was obtained from sugarcane based on the stem full-length cdna library. the scdir gene was 819-bp long, including a 564-bp orf encoding 187 amino acid residues. the protein n-terminus contained signal pep ...201222696141
chemical shift assignments of the canecystatin-1 from saccharum officinarum.cystatins are cysteine proteases inhibitors that are widely distributed among insects, mammalians and plants. here we report the complete resonance assignment of canecystatin-1 from saccharum officinarum obtained by heteronuclear multidimensional high-resolution nuclear magnetic resonance spectroscopy. the consensus chemical shift index was calculated and showed the presence of one α-helix (residues 27-43) and three β-strands (residues 48-74, 78-89 and 94-104), a secondary structure pattern that ...201322696137
a novel multienzyme complex from a newly isolated facultative anaerobic bacterium, paenibacillus sp. tw1.a multienzyme complex from newly isolated paenibacillus sp. tw1 was purified from pellet-bound enzyme preparations by elution with 0.25% sucrose and 1.0% triethylamine (tea), ultrafiltration and sephacryl s-400 gel filtration chromatography. the purified multienzyme complex showed a single protein band on non-denaturing polyacrylamide gel electrophoresis (native-page). the high molecular mass of the purified multienzyme complex was approximately 1,950 kda. the complex consisted of xylanase and c ...201222695526
extended monitoring of resistance to bacillus thuringiensis cry1ab maize in diatraea saccharalis (lepidoptera: crambidae).the sugarcane borer, diatraea saccharalis (f.), is a major target of transgenic maize expressing bacillus thuringiensis (bt) proteins in south america and the mid-southern region of the united states. during 2007-2009, a total of 986 feral individuals of d. saccharalis were collected from maize fields in six locations of louisiana and mississippi and examined for resistance to cry1ab maize using f 1/f 2 screens. major resistance alleles to cry1ab maize in the populations sampled from non-bt maiz ...201422688686
the nearctic-caribbean species leptotrachelus dorsalis (fabricius, 1801): larval descriptions with a diagnosis of immature ctenodactylini and natural history notes on the genus and tribe (coleoptera, carabidae).adults and larvae of leptotrachelus dorsalis (fabricius), the sugarcane savior beetle, live in association with grasses, the larvae in the appressed leaf axils. both adult and larval leptotrachelus dorsalis eat larvae of the sugarcane borer, diatraea saccharalis (fabricius), and perhaps other insects living in the confines of the leaf sheaths of that and other grass-like species. the geographic range of leptotrachelus dorsalis extends from kansas in the west to the atlantic seaboard, north as fa ...201222679382
epicoccum nigrum p16, a sugarcane endophyte, produces antifungal compounds and induces root growth.sugarcane is one of the most important crops in brazil, mainly because of its use in biofuel production. recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potential. epicoccum nigrum is an important sugarcane endophytic fungus that has been associated with the biological control of phytopathogens, and the production of secondary metabolites. in spite of several studies carried out to define ...201222675473
the type iii secretion system is necessary for the development of a pathogenic and endophytic interaction between herbaspirillum rubrisubalbicans and poaceae.herbaspirillum rubrisubalbicans was first identified as a bacterial plant pathogen, causing the mottled stripe disease in sugarcane. h. rubrisubalbicans can also associate with various plants of economic interest in a non pathogenic manner.201222672506
single cell oil of oleaginous fungi from the tropical mangrove wetlands as a potential feedstock for biodiesel.single cell oils (scos) accumulated by oleaginous fungi have emerged as a potential alternative feedstock for biodiesel production. though fungi from mangrove ecosystem have been reported for production of several lignocellulolytic enzymes, they remain unexplored for their sco producing ability. thus, these oleaginous fungi from the mangrove ecosystem could be suitable candidates for production of scos from lignocellulosic biomass. the accumulation of lipids being species specific, strain select ...201222646719
measurement and analysis of the mannitol partition coefficient in sucrose crystallization under simulated industrial conditions.mannitol is a major deterioration product of leuconstoc mesenteroides bacterial metabolism of sucrose and fructose from both sugarcane and sugar beet. the effect of crystallization conditions on the mannitol partition coefficient (k(eff)) between impure sucrose syrup and crystal has been investigated in a batch laboratory crystallizer and a batch pilot plant-scale vacuum pan. laboratory crystallization was operated at 65.5°c (150°f), 60.0°c (140°f), and 51.7°c (125°f) with a 78.0 brix (% refract ...201222632769
enrichment of genomic dna for polymorphism detection in a non-model highly polyploid crop plant.large polyploid genomes of non-model species remain challenging targets for dna polymorphism discovery despite the increasing throughput and continued reductions in cost of sequencing with new technologies. for these species especially, there remains a requirement to enrich genomic dna to discover polymorphisms in regions of interest because of large genome size and to provide the sequence depth to enable estimation of copy number. various methods of enriching dna have been utilised, but some re ...201222624722
development of novel chloroplast microsatellite markers for miscanthus species (poaceae).a set of novel chloroplast microsatellite markers (cpssrs) was developed for the bioenergy crop miscanthus, and their utility in cross-species amplification was evaluated.201222615308
use of simple sequence repeat markers for dna fingerprinting and diversity analysis of sugarcane (saccharum spp) cultivars resistant and susceptible to red rot.red rod is an economically important disease of sugarcane caused by the fungus colletotrichum falcatum. we used a simple sequence repeat (ssr)-based marker system to identify and analyze genetic relationships of red rot resistant and susceptible sugarcane cultivars grown in pakistan. twenty-one highly polymorphic ssr markers were used for dna fingerprinting and genetic diversity analysis of 20 sugarcane cultivars. these ssr markers were found to be highly robust; we identified 144 alleles, with ...201222614346
identification of sense and antisense transcripts regulated by drought in sugarcane.sugarcane is an important sugar and energy crop that can be used efficiently for biofuels production. the development of sugarcane cultivars tolerant to drought could allow for the expansion of plantations to sub-prime regions. knowledge on the mechanisms underlying drought responses and its relationship with carbon partition would greatly help to define routes to increase yield. in this work we studied sugarcane responses to drought using a custom designed oligonucleotide array with 21,901 diff ...201222610347
vermicomposting of milk processing industry sludge spiked with plant wastes.this work illustrates the vermistabilization of wastewater sludge from a milk processing industry (mpis) unit spiked with cow dung (cd), sugarcane trash (st) and wheat straw (ws) employing earthworms eisenia fetida. a total of nine experimental vermibeds were established and changes in chemical parameters of waste material have been observed for 90 days. vermistabilization caused significant reduction in ph, organic carbon and c:n ratio and substantial increase in total n, available p and exchan ...201222609678
high-temperature ethanol fermentation by immobilized coculture of kluyveromyces marxianus and saccharomyces cerevisiae.suspended and immobilized cocultures of the thermotolerant yeast, kluyveromyces marxianus dmku 3-1042 and the mesophilic flocculent yeast, saccharomyces cerevisiae m30 were studied for their abilities to improve production and stability of ethanol fermentation. sugarcane juice and blackstrap molasses, at initial sugar concentrations of 220 g/l, were used as carbon sources. the results indicated that the coculture system could improve ethanol production from both sugarcane juice and blackstrap mo ...201222608995
high poly(β-hydroxybutyrate) production by pseudomonas fluorescens a2a5 from inexpensive substrates.a strain of poly(β-hydroxybutyrate) (phb)-accumulating bacterium was isolated from the soil in alaska of usa, identified as pseudomonas fluorescens, and designated as strain a2a5. the organism grew at temperatures below 30°c, and accumulated a large amount of granules in its cells when it was cultured in the sugarcane liquor medium. the purified sample from cells was determined as phb by gas chromatographic and nuclear magnetic resonance analysis of polyesters. the cell density of the culture in ...200822578867
influence of sugarcane burning on indoor/outdoor pah air pollution in brazil.this work presents the influence of sugarcane burning on pah levels and their profiles at a residence located in araraquara (sp, brazil), a city surrounded by sugarcane plantations. the average concentrations of total pahs (σpahs) associated with atmospheric particulate matter were higher during the burning period (σpahs 22.9 ng m(-3)) than in the non-burning period (σpah 2.35 ng m(-3)). a comparison of our results with previous studies regarding pah levels and their profiles in araraquara outdo ...201222578797
biodegradation of s-triazine herbicide atrazine by enterobacter cloacae and burkholderia cepacia sp. from long-term treated sugarcane-cultivated soils in kenya.in this study soils from sugarcane-cultivated fields were screened for bacterial species capable of atrazine (6-chloro-n²-ethyl-n⁴-isopropyl-1,3,5-triazine-2,4-diamine) degradation due to long exposure of the soils to this herbicide. to enrich for atrazine degraders, minimal salt medium containing atrazine as the sole n source and glucose as the c source was inoculated with soils impacted with this herbicide and incubated. bacterial growth was monitored by measuring optical density. the degradat ...201222575004
haplotype structure around bru1 reveals a narrow genetic basis for brown rust resistance in modern sugarcane cultivars.modern sugarcane cultivars (saccharum spp., 2n = 100-130) are high polyploid, aneuploid and of interspecific origin. a major gene (bru1) conferring resistance to brown rust, caused by the fungus puccinia melanocephala, has been identified in cultivar r570. we analyzed 380 modern cultivars and breeding materials covering the worldwide diversity with 22 molecular markers genetically linked to bru1 in r570 within a 8.2 cm segment. our results revealed a strong ld in the bru1 region and strong assoc ...201222572763
whole-genome sequencing of the efficient industrial fuel-ethanol fermentative saccharomyces cerevisiae strain cat-1.the saccharomyces cerevisiae strains widely used for industrial fuel-ethanol production have been developed by selection, but their underlying beneficial genetic polymorphisms remain unknown. here, we report the draft whole-genome sequence of the s. cerevisiae strain cat-1, which is a dominant fuel-ethanol fermentative strain from the sugarcane industry in brazil. our results indicate that strain cat-1 is a highly heterozygous diploid yeast strain, and the ~12-mb genome of cat-1, when compared w ...201222562254
enzymatic activity and proteomic profile of class iii peroxidases during sugarcane stem development.class iii peroxidases are present as large multigene families in all land plants. this large number of genes together with the diversity of processes catalyzed by peroxidases suggests possible functional specialization of each isoform. however, assigning a precise role for each individual peroxidase gene has continued to be a major bottleneck. here we investigated the enzyme activity and translational profile of class iii peroxidases during stem development of sugarcane as a first step in the es ...201222551762
putative recombination signature and significance of insertion/deletion events in the rna-dependent rna polymerase coding region of sugarcane yellow leaf virus.the 5898 nucleotide single-strand rna genome of sugarcane yellow leaf virus (scylv) contains one long open reading frame, which is translated into a 120.6 kda polyprotein. the sequences of scylv isolates from the two scylv-susceptible cultivars from hawaii had a deletion of 48-51 nt in orf1. scylv from 12 sugarcane hybrid cultivars from different origins were tested by rt-pcr using a specific set of primers, to investigate the genome segment for this deletion. only three cultivars were found not ...201222542996
establishment of an in vitro regeneration system for genetic transformation of selected sugarcane genotypes.a good culture system provides considerable quantities of highly regenerable target tissues. embryogenic callus cultures are ideal for micro-projectile-mediated transformation, because regenerable cells are not very stable. effective exploitation of genetic transformation requires good regeneration systems. we selected three sugarcane genotypes for the establishment and optimization of good in vitro regeneration systems, viz., s-2003-us-359, s-2006-sp-30, and s-2003-us-165. three callus inductio ...201222535387
elephant grass, sugarcane, and rice bran in diets for confined sheep.we aimed to evaluate the effects of diets, based on elephant grass or sugarcane as roughage and corn meal or rice bran as energy concentrate, on performance and body composition in terms of diet intake and digestibility. a total of 30 santa ines crossbreds (sic), castrated male sheep with 19.8 ± 2.0 kg initial body weight (bw) were used. six animals were slaughtered at the onset of the experiment to estimate the initial body composition for the other animals. the remaining 24 animals were distri ...201222528534
relations between phenotypic changes of spores and biofilm production by bacillus atrophaeus atcc 9372 growing in solid-state fermentation.bacillus spp. spores are usually obtained from strains cultivated in artificial media. however, in natural habitats, spores are predominantly formed from bacteria present in highly surface-associated communities of cells. solid-state fermentation (ssf) is the culture method that best mimetizes the natural environment of many microorganisms that grow attached to the surface of solid particles. this study aims to confirm that sporulation through ssf of bacillus atrophaeus occurs by biofilm formati ...201222526268
a bac library of the sp80-3280 sugarcane variety (saccharum sp.) and its inferred microsynteny with the sorghum genome.sugarcane breeding has significantly progressed in the last 30 years, but achieving additional yield gains has been difficult because of the constraints imposed by the complex ploidy of this crop. sugarcane cultivars are interspecific hybrids between saccharum officinarum and saccharum spontaneum. s. officinarum is an octoploid with 2n = 80 chromosomes while s. spontaneum has 2n = 40 to 128 chromosomes and ploidy varying from 5 to 16. the hybrid genome is composed of 70-80% s. officinaram and 5- ...201222524198
rapid and simple dna extraction method for the detection of enterotoxigenic staphylococcus aureus directly from food samples: comparison of pcr and lamp methods.the study describes the development of simple and rapid dna extraction method in combination with loop-mediated isothermal amplification (lamp) to detect enterotoxigenic staphylococcus aureus in food samples.201222519925
plant growth-promoting nitrogen-fixing enterobacteria are in association with sugarcane plants growing in guangxi, china.the current nitrogen fertilization for sugarcane production in guangxi, the major sugarcane-producing area in china, is very high. we aim to reduce nitrogen fertilization and improve sugarcane production in guangxi with the help of indigenous sugarcane-associated nitrogen-fixing bacteria. we initially obtained 196 fast-growing bacterial isolates associated with the main sugarcane cultivar roc22 plants in fields using a nitrogen-deficient minimal medium and screened out 43 nitrogen-fixing isolate ...201222510648
feasibility of using an alternative larval host and host plants to establish cotesia flavipes (hymenoptera: braconidae) in the temperate louisiana sugarcane ecosystem.attempts to establish cotesia flavipes cameron (hymenoptera: braconidae) in louisiana sugarcane fields to control the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae) have been unsuccessful. experiments were conducted to investigate the feasibility of using an alternative larval host and host plants to overcome barriers preventing establishment. in addition, we evaluated c. flavipes' ability to search for d. saccharalis in sugarcane without above-ground internodes. diatraea ev ...201222506999
production of cellulases from aspergillus niger ns-2 in solid state fermentation on agricultural and kitchen waste residues.various agricultural and kitchen waste residues were assessed for their ability to support the production of a complete cellulase system by aspergillus niger ns-2 in solid state fermentation. untreated as well as acid and base-pretreated substrates including corn cobs, carrot peelings, composite, grass, leaves, orange peelings, pineapple peelings, potato peelings, rice husk, sugarcane bagasse, saw dust, wheat bran, wheat straw, simply moistened with water, were found to be well suited for the or ...201222503148
characterization of laccase isoforms produced by pleurotus ostreatus in solid state fermentation of sugarcane bagasse.laccases are oxidative enzymes linked to biological degradation of lignin. the aim of this work was to evaluate the effect of inducers and different concentrations of nitrogen on production level of total laccase activity and pattern of laccase isoforms, produced in solid state fermentation of sugarcane bagasse by a selected strain of pleurotus ostreatus. the addition of yeast extract 5 g/l, copper sulfate 150 μm and ferulic acid 2 mm provided highest enzymatic activity (167 u/g) and zymograms i ...201222487128
genetic variability and potential recombination events in the hc-pro gene of sugarcane streak mosaic virus.sugarcane streak mosaic virus (scsmv), a member of the family potyviridae, is an important viral pathogen affecting sugarcane production in india. the variability in the nucleotide (nt) and amino acid (aa) sequences of helper component proteinase (hc-pro) of scsmv isolates from india was investigated and compared with those of previously published virus isolates from different asian countries. comparison of all of the sequenced virus isolates revealed a high level of diversity in the hc-pro gene ...201222481599
influence of different supplements and sugarcane (saccharum officinarum l.) cultivars on intake, digestible variables and methane production of dairy heifers under tropical conditions.the sulphur hexafluoride (sf(6)) gas tracer method was used to measure methane (ch(4)) production of crossbred (3/4 holstein x zebu) dairy heifers fed two types of sugarcane (saccharum officinarum l.; cultivar iac-862480 (cc1) or cultivar iac-873184 (cc2)) and supplemented with urea or concentrate. the study was performed at embrapa southeast cattle, são carlos, sp, brazil, using a completely randomised design. differences between treatments were significant for digestibility of dry matter, orga ...201222476735
larval habitat associations with human land uses, roads, rivers, and land cover for anopheles albimanus, a. pseudopunctipennis, and a. punctimacula (diptera: culicidae) in coastal and highland ecuador.larval habitat for three highland anopheles species: anopheles albimanus wiedemann, anopheles pseudopunctipennis theobald, and anopheles punctimacula dyar and knab was related to human land uses, rivers, roads, and remotely sensed land cover classifications in the western ecuadorian andes. of the five commonly observed human land uses, cattle pasture (n = 30) provided potentially suitable habitat for a. punctimacula and a. albimanus in less than 14% of sites, and was related in a principal compo ...201222454623
sequential solid-state and submerged cultivation of aspergillus niger on sugarcane bagasse for the production of cellulase.sequential solid-state and submerged cultivation with sugarcane bagasse as substrate for cellulase production by aspergillus niger a12 was assessed by measuring endoglucanase activity. an unconventional pre-culture with an initial fungal growth phase under solid-state cultivation was followed by a transition to submerged fermentation by adding the liquid culture medium to the mycelium grown on solid substrate. for comparison, control experiments were conducted using conventional submerged cultiv ...201222409979
mass balance of pilot-scale pretreatment of sugarcane bagasse by steam explosion followed by alkaline delignification.five pilot-scale steam explosion pretreatments of sugarcane bagasse followed by alkaline delignification were explored. the solubilised lignin was precipitated with 98% sulphuric acid. most of the pentosan (82.6%), and the acetyl group fractions were solubilised during pretreatment, while 90.2% of cellulose and 87.0% lignin were recovered in the solid fraction. approximately 91% of the lignin and 72.5% of the pentosans contained in the steam-exploded solids were solubilised by delignification, r ...201222391588
production of a his-tagged canecystatin in transgenic sugarcane.transgenic plants have been widely used as expression systems of recombinant proteins in recent years because it can be an efficient alternative for the large-scale production of proteins. this is an area with great potential but is still not much explored. indeed, this system can bring a breakthrough in the expression of any protein. the model used here as a protein factory was sugarcane, a crop of great global importance. this chapter describes the system that has been adopted in the routine p ...201222351027
lactobacillus reuteri crl 1101 highly produces mannitol from sugarcane molasses as carbon source.mannitol is a natural polyol extensively used in the food industry as low-calorie sugar being applicable for diabetic food products. we aimed to evaluate mannitol production by lactobacillus reuteri crl 1101 using sugarcane molasses as low-cost energy source. mannitol formation was studied in free-ph batch cultures using 3-10% (w/v) molasses concentrations at 37 °c and 30 °c under static and agitated conditions during 48 h. l. reuteri crl 1101 grew well in all assayed media and heterofermentativ ...201222350320
identification of n-acyl homoserine lactones produced by gluconacetobacter diazotrophicus pal5 cultured in complex and synthetic media.the endophytic diazotrophic gluconacetobacter diazotrophicus pal5 was originally isolated from sugarcane (saccharum officinarum). the biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. the recent genomic sequence of pal5 unveiled the presence of a quorum sensing (qs) system. qs are regulatory mechanisms that, through the production of signal molecules or autoinduce ...201222350020
possible involvement of maize rop1 in the defence responses of plants to viral infection.the expression of host genes can be altered during the process of viral infection. to investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with sugarcane mosaic virus (scmv), a suppression subtractive hybridization cdna library was constructed. a total of 454 cdna clones were identified to be viral infection-induced up-regulated genes. the influence of rop1 on the infection of maize by scmv was investigated. the result ...201222332840
understanding physiological responses to pre-treatment inhibitors in ethanologenic fermentations.alcohol-based liquid fuels feature significantly in the political and social agendas of many countries, seeking energy sustainability. it is certain that ethanol will be the entry point for many sustainable processes. conventional ethanol production using maize- and sugarcane-based carbohydrates with saccharomyces cerevisiae is well established, while lignocellulose-based processes are receiving growing interest despite posing greater technical and scientific challenges. a significant challenge ...201222331581
production of thermophilic endo-β-1,4-xylanases by aspergillus fumigatus fbspe-05 using agro-industrial by-products.in the present paper, endo-β-1,4-xylanase production by aspergillus fumigatus was evaluated in solid-state fermentation using low-cost substrates such as sugarcane bagasse (scb), brewer's spent grain (bsg), and wheat bran (wb). the partial characterization of the crude enzyme was also performed. in the experimental conditions, the highest levels of endo-β-1,4-xylanase production by a. fumigatus fbspe-05 occurred within 8 days incubation when using scb/liquid medium at 1:2 ratio (219.5 u g(-1)) a ...201222328248
study of postures in sugarcane cutters in the pontal of paranapanema-sp, brazil.the expansion of sugarcane monoculture in brazil in the last decades has pointed out to the necessity of considering the question of sugarcane cutters occupational health. in this work we present a cross-sectional study aiming to examine the occupational posture of a group of sugarcane cutters, which work in a cane field located in the region of pontal do paranapanema- sp, brazil. the study was made using the ergonomic analysis of work - eaw methodology and the postural analysis method by win-ow ...201222317560
impacts of introducing a new tool for the manual harvesting of sugar cane: the ergonomic analysis contribution.the purpose of this study was to evaluate the impact of introduction a new work tool on health and productivity of manual harvesting sugarcane workers. in this study it was performed aergonomic work analysis (ewa) to analyze the work tool changes.the study included sugarcane workers, drivers, fiscals, and technical's safety at work.during the analysis process, 134 questionnaires were applied,aimed to identify the constraints that such workers are submitted,to evaluate the tool introduced, in ord ...201222317488
protective gloves on manual sugar cane cutting are really effective?problems related to the use of personal protective equipment (ppe), specially the use of protective gloves for the manual sugar cane cutting, motivated this research, made possible by a tripartite negotiation involving the ministry of labor, the union of rural workers and the employer's association of sugarcane agribusiness. the main objective was to evaluate, from an ergonomics perspective, the impact of use of the gloves during the manual cane sugar cutting, raising questions on safety, effect ...201222317487
genotypic and phenotypic diversity of pgpr fluorescent pseudomonads isolated from the rhizosphere of sugarcane (saccharum officinarum l.).the genetic diversity of plant growth-promoting rhizobacterial (pgpr) fluorescent pseudomonads associated with the sugarcane (saccharum officinarum l.) rhizosphere was analyzed. selected isolates were screened for plant growthpromoting properties including production of indole acetic acid, phosphate solubilization, denitrification ability, and production of antifungal metabolites. furthermore, 16s rdna sequence analysis was performed to identify and differentiate these isolates. based on 16s rdn ...201222297215
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