Publications

comparative raman study of four plant metallothionein isoforms: insights into their zn(ii) clusters and protein conformations.	four metallothioneins (mts) from soybean (glycine max) were heterologously synthesized and comparatively analysed by raman spectroscopy. the participation of protein donor groups (s-thiol and n-imidazol) in zn(ii) chelation, as well as the presence of secondary structure elements was comparatively analysed. metal clusters with different geometry can be hypothesised for the four gmmts: a cubane-like or an adamantane-like metal cluster in zn-gmmt1, and dinuclear zn-s clusters in zn-gmmt2, zn-gmmt3 ...	2016	26775276
biocontrol potential of trichoderma harzianum isolate t-aloe against sclerotinia sclerotiorum in soybean.	sclerotinia stem rot, caused by sclerotinia sclerotiorum (lib.) de bary is a major disease of soybean (glycine max (l.) merr.). at present, we revealed the three-way interaction between trichoderma harzianum t-aloe, pathogen s. sclerotiorum and soybean plants in order to demonstrate biocontrol mechanism and evaluate biocontrol potential of t-aloe against s. sclerotiorum in soybean. in our experiments, t-aloe inhibited the growth of s. sclerotiorum with an efficiency of 56.3% in dual culture test ...	2016	26774866
enhanced single seed trait predictions in soybean (glycine max) and robust calibration model transfer with near-infrared reflectance spectroscopy.	single seed near-infrared reflectance (nir) spectroscopy predicts soybean (glycine max) seed quality traits of moisture, oil, and protein. we tested the accuracy of transferring calibrations between different single seed nir analyzers of the same design by collecting nir spectra and analytical trait data for globally diverse soybean germplasm. x-ray microcomputed tomography (μct) was used to collect seed density and shape traits to enhance the number of soybean traits that can be predicted from ...	2016	26771201
genome-wide transcriptome analysis of soybean primary root under varying water-deficit conditions.	soybean is a major crop that provides an important source of protein and oil to humans and animals, but its production can be dramatically decreased by the occurrence of drought stress. soybeans can survive drought stress if there is a robust and deep root system at the early vegetative growth stage. however, little is known about the genome-wide molecular mechanisms contributing to soybean root system architecture. this study was performed to gain knowledge on transcriptome changes and related ...	2016	26769043
loci and candidate gene identification for resistance to phytophthora sojae via association analysis in soybean [glycine max (l.) merr].	phytophthora sojae is an oomycete soil-borne plant pathogen that causes the serious disease phytophthora root rot in soybean, leading to great loss of soybean production every year. understanding the genetic basis of this plant-pathogen interaction is important to improve soybean disease resistance. to discover genes or qtls underlying naturally occurring variations in soybean p.sojae resistance, we performed a genome-wide association study using 59,845 single-nucleotide polymorphisms identified ...	2016	26758588
estimation of cotyledon isoflavone abundance by a grey luminance-based model in variously hilum-coloured soybean varieties.	the nutraceutical uses of soybean (glycine max l. merr.) have received increasing attention in recent years, due to the therapeutic effects of high seed isoflavone concentrations against heart disease, cancer and menopausal symptoms.	2016	26757482
oral challenge tests for soybean allergies in japan: a summary of 142 cases.	soybeans are one of causative foods for infantile onset allergies in japan. this study aimed to analyze the results of soybean challenge tests that were conducted over approximately 7 years at our institution. using the test data, we sought to identify the responses and clinical profiles of patients with soybean allergies, and to investigate the relationship between the responses and soybean sensitization status.	2016	26740299
toxicological assessment of nattokinase derived from bacillus subtilis var. natto.	subtilisin nat, commonly known as "nattokinase," is a fibrinolytic enzyme produced by the bacterial strain b. subtilis var. natto, which plays a central role in the fermentation of soybeans into the popular japanese food natto. recent studies have reported on the potential anticoagulatory and antihypertensive effects of nattokinase administration in humans, with no indication of adverse effects. to evaluate the safety of nattokinase in a more comprehensive manner, several glp-compliant studies i ...	2016	26740078
construction of high resolution genetic linkage maps to improve the soybean genome sequence assembly glyma1.01.	a landmark in soybean research, glyma1.01, the first whole genome sequence of variety williams 82 (glycine max l. merr.) was completed in 2010 and is widely used. however, because the assembly was primarily built based on the linkage maps constructed with a limited number of markers and recombinant inbred lines (rils), the assembled sequence, especially in some genomic regions with sparse numbers of anchoring markers, needs to be improved. molecular markers are being used by researchers in the s ...	2016	26739042
genome-wide identification of calcium-dependent protein kinases in soybean and analyses of their transcriptional responses to insect herbivory and drought stress.	calcium-dependent protein kinases (cdpks) are plant-specific calcium sensors that play important roles in various aspects of plant physiology. here, we investigated phylogenic relationships, chromosomal locations, gene structures, and tissue-specific, herbivory- and drought-induced expression profiles of soybean (glycine max) gmcdpks. fifty gmcdpk genes were identified, which phylogenetically grouped into 4 distinct clusters and distributed across 13 sub-clusters. individual classes of gmcdpks h ...	2016	26733237
fumonisins and fungi in dry soybeans (glycine max l.) for human consumption.	this survey reports the occurrence of fumonisins (fbs) and fungi in dry soybeans sold for human consumption. the variation levels were 138-1495 µg kg(-1) and 178-552 µg kg(-1) for fb1 and fb2, respectively. in addition, potentially toxigenic fungi as fusarium, aspergillus and penicillium genera were isolated in the samples. these can be considered as indicator-toxin and can produce considerable amounts of mycotoxins. despite fb presence in the soybeans for human consumption, there is no legal re ...	2016	26727078
pyramids of qtls enhance host-plant resistance and bt-mediated resistance to leaf-chewing insects in soybean.	qtl-m and qtl-e enhance soybean resistance to insects. pyramiding these qtls with cry1ac increases protection against bt-tolerant pests, presenting an opportunity to effectively deploy bt with host-plant resistance genes. plant resistance to leaf-chewing insects minimizes the need for insecticide applications, reducing crop production costs and pesticide concerns. in soybean [glycine max (l.) merr.], resistance to a broad range of leaf-chewing insects is found in pi 229358 and pi 227687. pi 2293 ...	2016	26724806
diversity of seed cesium accumulation in soybean mini-core collections.	radiocesium is an extremely harmful radionuclide because of its long half-life; it is important to reduce its transfer from contaminated soil into crops. here we surveyed genetic variation for seed cesium (cs) concentration in soybean mini-core collections representing large genetic diversity. the collections grown over 3 years in rotational paddy fields exhibited varying seed cs concentrations with significant year-to-year correlations, although the phenotypic stability of cs concentration was ...	2015	26719739
a 14-3-3 family protein from wild soybean (glycine soja) regulates aba sensitivity in arabidopsis.	it is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. by conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in aba responses. hence, in this study, based on the latest glycine max genome on phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, ...	2015	26717241
nutrients limiting soybean (glycine max l) growth in acrisols and ferralsols of western kenya.	low soybean yields in western kenya have been attributed to low soil fertility despite much work done on nitrogen (n) and phosphorus (p) nutrition leading to suspicion of other nutrient limitations. to investigate this, a nutrient omission trial was set up in the greenhouse at the university of eldoret-kenya to diagnose the nutrients limiting soybean production in acrisols from masaba central and butere sub-counties, and ferralsols from kakamega (shikhulu and khwisero sub-locations) and butula s ...	2015	26716825
global investigation of the co-evolution of mirna genes and microrna targets during soybean domestication.	although the selection of coding genes during plant domestication has been well studied, the evolution of mirna genes (mirs) and the interaction between micrornas (mirnas) and their targets in this process are poorly understood. here, we present a genome-wide survey of the selection of mirs and mirna targets during soybean domestication and improvement. our results suggest that, overall, mirs have higher evolutionary rates than mirna targets. nonetheless, they do demonstrate certain similar evol ...	2016	26714457
isolation and characterization of "gmscream" promoters that regulate highly expressing soybean (glycine max merr.) genes.	to increase our understanding of the regulatory components that control gene expression, it is important to identify, isolate and characterize new promoters. in this study, a group of highly expressed soybean (glycine max merr.) genes, which we have named "gmscream", were first identified from rna-seq data. the promoter regions were then identified, cloned and fused with the coding region of the green fluorescent protein (gfp) gene, for introduction and analysis in different tissues using 3 tool ...	2015	26706070
rat and poultry feeding studies with soybean meal produced from imidazolinone-tolerant (cv127) soybeans.	the safety and nutritional properties of cv127 soybeans were evaluated in rat and broiler feeding studies. some episodic differences were observed between rats fed cv127, conquista, and the standard diet for the endpoints examined. none of these differences were considered treatment related, adverse, or biologically meaningful. in general, birds fed diets containing cv127, conquista, or monsoy 8001 showed no significant differences in growth and performance response variables. chickens fed diets ...	2016	26699944
contrails: a tool for rapid identification of transgene integration sites in complex, repetitive genomes using low-coverage paired-end sequencing.	transgenic crops have become a staple in modern agriculture, and are typically characterized using a variety of molecular techniques involving proteomics and metabolomics. characterization of the transgene insertion site is of great interest, as disruptions, deletions, and genomic location can affect product selection and fitness, and identification of these regions and their integrity is required for regulatory agencies. here, we present contrails (characterization of transgene insertion locati ...	2015	26697366
identification of differentially expressed genes between developing seeds of different soybean cultivars.	soybean is a major source of protein and oil and a primary feedstock for biodiesel production. research on soybean seed composition and yield has revealed that protein, oil and yield are controlled quantitatively and quantitative trait loci (qtl) have been identified for each of these traits. however, very limited information is available regarding the genetic mechanisms controlling seed composition and yield. to help address this deficiency, we used affymetrix soybean genechips® to identify gen ...	2015	26697344
identification of protein-protein interactions of isoflavonoid biosynthetic enzymes with 2-hydroxyisoflavanone synthase in soybean (glycine max (l.) merr.).	metabolic enzymes, including those involved in flavonoid biosynthesis, are proposed to form weakly bound, ordered protein complexes, called "metabolons". some hypothetical models of flavonoid biosynthetic metabolons have been proposed, in which metabolic enzymes are believed to anchor to the cytoplasmic surface of the endoplasmic reticulum (er) via er-bound cytochrome p450 isozymes (p450s). however, no convincing evidence for the interaction of flavonoid biosynthetic enzymes with p450s has been ...	2016	26694697
fusarium paranaense sp. nov., a member of the fusarium solani species complex causes root rot on soybean in brazil.	isolates of fusarium obtained from soybean plants showing symptoms of root rot collected in subtropical southern and tropical central brazil were characterized based on phylogenetic analyses, sexual crossing, morphology, and pathogenicity tests. a novel species within the fusarium solani species complex (fssc) causing soybean root rot is formally described herein as fusarium paranaense. this species can be distinguished from the other soybean root rot pathogens in the fssc, which are commonly as ...	2016	26693684
the interaction of salicylic acid and ca(2+) alleviates aluminum toxicity in soybean (glycine max l.).	both calcium ion (ca(2+)) and salicylic acid (sa) influence various stress responses in plants. in acidic soils, aluminum (al) toxicity adversely affects crop yield. in this study, we determined the influences of ca(2+) and sa on root elongation, al accumulation, and citrate secretion in soybean plant. we also investigated the activity of antioxidative enzymes in al-exposed soybean roots. root elongation was severally inhibited when the roots were exposed to 30 μm al. the al-induced inhibition o ...	2016	26691059
ureases as multifunctional toxic proteins: a review.	ureases are metalloenzymes that hydrolyze urea into ammonia and carbon dioxide. they were the first enzymes to be crystallized and, with them, the notion that enzymes are proteins became accepted. novel toxic properties of ureases that are independent of their enzyme activity have been discovered in the last three decades. since our first description of the neurotoxic properties of canatoxin, an isoform of the jack bean urease, which appeared in toxicon in 1981, about one hundred articles have b ...	2016	26690979
preparation and application of a monoclonal antibody against the isoflavone glycoside daidzin using a mannich reaction-derived hapten conjugate.	daidzin and its aglycone daidzein are major pharmacologically active compounds of soybean (glycine max), kudzu (pueraria lobata), and kwao kruea khao (p. mirifica). pharmacological activities of daidzin are mediated by its more potent metabolites daidzein and equol; however, daidzin is the predominant compound found in these medicinal plants, and the efficacy and safety of equol depend on the amount of daidzin consumed.	2016	26689919
transcriptome profiling of soybean (glycine max) roots challenged with pathogenic and non-pathogenic isolates of fusarium oxysporum.	fusarium oxysporum is one of the most common fungal pathogens causing soybean root rot and seedling blight in u.s.a. in a recent study, significant variation in aggressiveness was observed among isolates of f. oxysporum collected from roots in iowa, ranging from highly pathogenic to weakly or non-pathogenic isolates.	2015	26689712
arsenic toxicity in soybean seedlings and their attenuation mechanisms.	even though vast areas contaminated with arsenic (as) are under soybean (glycine max) cultivation, little is known about the growth and intrinsic antioxidant metabolism of soybean in response to as exposure. thus, an evaluation was carried out of plant growth, root anatomy, antioxidant system and photosynthetic pigment content under arsenate (as(v)) and arsenite (as(iii)) treatment. soybean seedling growth was significantly affected at 25 μm or higher concentrations of as(v) or as(iii), and the ...	2016	26686284
soybean aphid infestation induces changes in fatty acid metabolism in soybean.	the soybean aphid (aphis glycines matsumura) is one of the most important insect pests of soybeans in the north-central region of the us. it has been hypothesized that aphids avoid effective defenses by inhibition of jasmonate-regulated plant responses. given the role fatty acids play in jasmonate-induced plant defenses, we analyzed the fatty acid profile of soybean leaves and seeds from aphid-infested plants. aphid infestation reduced levels of polyunsaturated fatty acids in leaves with a conco ...	2015	26684003
genetic analysis reveals the essential role of nitrogen phosphotransferase system components in sinorhizobium fredii ccbau 45436 symbioses with soybean and pigeonpea plants.	the nitrogen phosphotransferase system (pts(ntr)) consists of ei(ntr), npr, and eiia(ntr). the active phosphate moiety derived from phosphoenolpyruvate is transferred through ei(ntr) and npr to eiia(ntr). sinorhizobium fredii can establish a nitrogen-fixing symbiosis with the legume crops soybean (as determinate nodules) and pigeonpea (as indeterminate nodules). in this study, s. fredii strains with mutations in ptsp and ptso (encoding ei(ntr) and npr, respectively) formed ineffective nodules on ...	2016	26682851
genome-wide transcriptome analyses of developing seeds from low and normal phytic acid soybean lines.	low phytic acid (lpa) crops are potentially eco-friendly alternative to conventional normal phytic acid (pa) crops, improving mineral bioavailability in monogastric animals as well as decreasing phosphate pollution. the lpa crops developed to date carry mutations that are directly or indirectly associated with pa biosynthesis and accumulation during seed development. these lpa crops typically exhibit altered carbohydrate profiles, increased free phosphate, and lower seedling emergence, the latte ...	2015	26678836
the effect of application of micromycetes on plant growth, as well as soybean and barley yields.	the possibility of application of micromycetes (strains penicillium bilaiae pb14, aureobasidium pullulans ya05 and rhodotorula mucilaginosa yr07) to increase yields of soybean (glycine max cv almaty) and barley (hordeum vulgare cv arna) was estimated. it was shown that the most positive effect on germination energy and seed germination after seed treatment with liquid culture, supernatant and filtrate, is achieved at 1:5 dilution. in studying the influence of cell-associated and extracellular bi ...	2015	26677462
response of soybean genotypes challenged by a stink bug complex (hemiptera: pentatomidae).	pentatomids (stink bugs) are major pests of soybean, glycine max (l.) merril. these pests reach high levels of infestation, cause severe damage to seeds by feeding, are linked to leaf retention, and are difficult to control. host plant resistance is considered to be a valuable tool in integrated pest management and can assist in reducing the damage caused by stink bugs. this research evaluated the resistance of soybean genotypes in brazil to the stink bug complex, the neotropical brown stink bug ...	2016	26662735
gain of virulence by soybean mosaic virus on rsv4-genotype soybeans is associated with a relative fitness loss in a susceptible host.	'gene-for-gene' theory predicts that gain of virulence by an avirulent pathogen on plants expressing resistance (r) genes is associated with fitness loss in susceptible hosts. however, the validity of this prediction has been studied in only a few plant viral pathosystems. in this study, the soybean mosaic virus (smv)-rsv4 pathosystem was exploited to test this prediction. in rsv4-genotype soybeans, p3 of avirulent smv strains provokes an as yet uncharacterized resistance mechanism that restrict ...	2016	26662495
a transgenic, visual screenable marker for soybean seeds.	most soybean cultivars produce buff colored seeds due to a seed coat specific sirna mechanism. this phenomenon is specifically limited to the seed coat and produces a strong visual effect, thus, a strategy to evade the silencing was used to produce a maternal transgenic marker for soybeans. expression of a rice chalcone synthase transgene with little dna sequence homology to the soybean sirnas resulted in dark colored seed coats. this phenotype is the result of anthocyanin pigment production and ...	2016	26660729
identification and molecular mapping of rps11, a novel gene conferring resistance to phytophthora sojae in soybean.	rps11 confers excellent resistance to predominant phytophthora sojae isolates capable of defeating major rps genes deployed into soybean production, representing a novel source of resistance for soybean cultivar enhancement.	2016	26660465
total synthesis of (±)-glyceollin ii and a dihydro derivative.	stressed soybeans produce a group of phytoalexins that belong to the 6a-hydroxypterocarpan family of flavonoids. certain of the more prominent members, such as the glyceollins i, ii, and iii, have demonstrated potential antidiabetic properties and promising cytotoxicity in both human breast and prostate cancer cell cultures with preliminary studies in animals further demonstrating antitumor effects in estrogen-dependent, human breast cancer cell implants. although syntheses of glyceollin i have ...	2015	26654660
effect of a long chain n-3 pufa-enriched diet on production and reproduction variables in holstein dairy cows.	the objective of this study was to determine the effect of a rumen-protected fish oil supplement on the production and reproduction variables in postpartum dairy cows. holstein cows (n=46) were given a basal total mixed diet plus one pufa supplement: n-3 (n-3; protected fish oil; 1% dry matter intake (dmi); n=23) or control (n-6; toasted soybeans; 1.8% dmi; n=23), in a switchback design over two consecutive lactations. supplements were added to the diet between calving and 2 months after calving ...	2016	26651949
genome-wide analysis of the basic leucine zipper (bzip) transcription factor gene family in six legume genomes.	plant bzip proteins characteristically harbor a highly conserved bzip domain with two structural features: a dna-binding basic region and a leucine (leu) zipper dimerization region. they have been shown to be diverse transcriptional regulators, playing crucial roles in plant development, physiological processes, and biotic/abiotic stress responses. despite the availability of six completely sequenced legume genomes, a comprehensive investigation of bzip family members in legumes has yet to be pr ...	2015	26651343
identification of haplotypes at the rsv4 genomic region in soybean associated with durable resistance to soybean mosaic virus.	discovery of new germplasm sources and identification of haplotypes for the durable soybean mosaic virus resistance gene, rsv 4, provide novel resources for map-based cloning and genetic improvement efforts in soybean. the soybean mosaic virus (smv) resistance locus rsv4 is of interest because it provides a durable type of resistance in soybean [glycine max (l.) merr.]. to better understand its molecular basis, we used a population of 309 bc3f2 individuals to fine-map rsv4 to a ~120 kb interval ...	2016	26649868
fruit and vegetable consumption and the incidence of hypertension in three prospective cohort studies.	increased fruit and vegetable intake lowers blood pressure in short-term interventional studies. however, data on the association of long-term intake of fruits and vegetables with hypertension risk are scarce. we prospectively examined the independent association of whole fruit (excluding juices) and vegetable intake, as well as the change in consumption of whole fruits and vegetables, with incident hypertension in 3 large longitudinal cohort studies: nurses' health study (n=62 175), nurses' hea ...	2016	26644239
effect of steam-flaked corn and soybeans on muscle and intramuscular fatty acid composition in holstein calves.	this study aimed to evaluate the effects of steam-flaked corn grains and soybeans on muscle fatty acid composition. thirty holstein bull calves (21 ± 3 d) were divided into 3 groups according to birth date and bw and were randomly assigned to receive fresh milk and a commercial pelleted starter diet containing extruded corn and soybean (ecs), steam-flaked corn and soybean (sfcs), or ground corn and raw soybean (gcs). the calves were fed the designated diet from 3 to 13 wk of age, after which the ...	2015	26641191
directed evolution of tau class glutathione transferases reveals a site that regulates catalytic efficiency and masks co-operativity.	a library of tau class gsts (glutathione transferases) was constructed by dna shuffling using the dna encoding the glycine max gsts gmgstu2-2, gmgstu4-4 and gmgstu10-10. the parental gsts are >88% identical at the sequence level; however, their specificity varies towards different substrates. the dna library contained chimaeric structures of alternated segments of the parental sequences and point mutations. chimaeric gst sequences were expressed in escherichia coli and their enzymatic activities ...	2016	26637269
overexpression of soybean isoflavone reductase (gmifr) enhances resistance to phytophthora sojae in soybean.	isoflavone reductase (ifr) is an enzyme involved in the biosynthetic pathway of isoflavonoid phytoalexin in plants. ifrs are unique to the plant kingdom and are considered to have crucial roles in plant response to various biotic and abiotic environmental stresses. here, we report the characterization of a novel member of the soybean isoflavone reductase gene family gmifr. overexpression of gmifr transgenic soybean exhibited enhanced resistance to phytophthora sojae. following stress treatments, ...	2015	26635848
antioxidant activity and anti-inflammatory activity of ethanol extract and fractions of doenjang in lps-stimulated raw 264.7 macrophages.	fermentation can increase functional compounds in fermented soybean products, thereby improving antioxidant and/or anti-inflammatory activities. we investigated the changes in the contents of phenolics and isoflavones, antioxidant activity and anti-inflammatory activity of doenjang during fermentation and aging.	2015	26634044
nutritional value of raw soybeans, extruded soybeans, roasted soybeans and tallow as fat sources in early lactating dairy cows.	thirty multiparous holstein cows (29.8 ± 4.01days in milk; 671.6 ± 31.47 kg of body weight) were used in a completely randomized design to compare nutritional value of four fat sources including tallow, raw soybeans, extruded soybeans and roasted soybeans for 8 weeks. experimental diets were a control containing 27.4 % alfalfa silage, 22.5% corn silage, and 50.1% concentrate, and four diets with either tallow, raw soybean, extruded soybean, or roasted soybean added to provide 1.93% supplemental ...	2012	26623299
comparison of fluoride effects on germination and growth of zea mays, glycine max and sorghum vulgare.	fluorosis is a disease caused by over-exposure to fluoride (f). argentina's rural lands have higher fluorine content than urban lands. evidence confirms that plants grown in fluoridated areas could have higher f content. we compared f uptake and growth of crops grown in different f concentrations. the effect of 0-8 ppm f concentrations on maize, soybeans and sorghum germination and growth was compared. after 6 days seeding, the germination was determined, the roots and aerial parts lengths were ...	2016	26621612
real-time pcr-based quantitation method for the genetically modified soybean line gts 40-3-2.	this chapter describes a real-time pcr-based method for quantitation of the relative amount of genetically modified (gm) soybean line gts 40-3-2 [roundup ready(®) soybean (rrs)] contained in a batch. the method targets a taxon-specific soybean gene (lectin gene, le1) and the specific dna construct junction region between the petunia hybrida chloroplast transit peptide sequence and the agrobacterium 5-enolpyruvylshikimate-3-phosphate synthase gene (epsps) sequence present in gts 40-3-2. the metho ...	2016	26614294
effect of sequential bio-processing conditions on the content and composition of vitamin k2 and isoflavones in fermented soy food.	in the present research, effect of sequential addition of bifidobacterium bifidum, bacillus subtilis and rhizopus oligosporus on content and composition of vitamin k2 and isoflavones in fermented soy foods have been investigated. initially, soybeans were fermented with b. bifidum; then this fermented mass was re-fermented with co-culture of b. subtilis and r. oligosporus. the evolved sequence of microbes inoculation tended towards significantly (p < 0.5) higher enzymes levels (126.16 ± 2.23 iu/m ...	2015	26604398
modulatory role of jasmonic acid on photosynthetic pigments, antioxidants and stress markers of glycine max l. under nickel stress.	jasmonic acid (ja) is a very young candidate of plant growth regulators which is being explored for various antistress properties. present study deals with the hypothesis that ja can modulate antioxidant mechanism of higher plants with tight regulation of biomembrane peroxidation, making plants tolerant to toxic ni(2+). 2 mm nicl2 as a source of ni(2+) appeared as sub lethal dose for the growth of 15 days old glycine max seedlings. exogenous application of 1 μm and 1 nm ja prior to nicl2 exposur ...	2015	26600682
development of primer pairs from diverse chloroplast genomes for use in plant phylogenetic research.	variation in the chloroplast dna sequence is useful for plant phylogenetic studies. however, the number of variable sequences provided by chloroplast dna for suggested genes or genomic regions in plant phylogenetic analyses is often inadequate. to identify conserved regions that can be used to design primers and amplify variable sequences for use in plant phylogenetic studies, the complete chloroplast genomic sequences of six plant species (including oryza sativa, arabidopsis thaliana, glycine m ...	2015	26600547
a perspective of the research on food factors in human health: a return to the mechanistic approach.	food is a mixture of several nutrients and non-nutrients. the functions of food in human health can be categorized as its primary function in nutrition, its secondary function in palatability and its tertiary function in bioregulation. nutrients contribute mainly to the primary function, while many non-nutrients in foods and foodstuffs are strongly associated with the secondary and/or tertiary functions. individual food ingredients and ingredients as a whole are the so-called food factors. much ...	2015	26598906
cadmium affects microtubule organization and post-translational modifications of tubulin in seedlings of soybean (glycine max l.).	cadmium (cd) is a non-essential heavy metal, toxic to all living organisms. the microtubule (mt) cytoskeleton appears to be one of the main targets of cd action. in this study we present, with the use of various immunological approaches, the effect of cd at moderate (85 μm) and high (170 μm) concentrations on the structure and functioning of the mt cytoskeleton in the root cells of soybean seedlings. as the result of heavy metal action, root growth was significantly diminished and was accompanie ...	2015	26594217
comparative analysis of chemical compositions between non-transgenic soybean seeds and those from plants over-expressing atjmt, the gene for jasmonic acid carboxyl methyltransferase.	transgenic overexpression of the arabidopsis gene for jasmonic acid carboxyl methyltransferase (atjmt) is involved in regulating jasmonate-related plant responses. to examine its role in the compositional profile of soybean (glycine max), we compared the seeds from field-grown plants that over-express atjmt with those of the non-transgenic, wild-type (wt) counterpart. our analysis of chemical compositions included proximates, amino acids, fatty acids, isoflavones, and antinutrients. overexpressi ...	2016	26593488
early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack.	southern green stink bugs (nezara viridula l.) invade field-grown soybean crops, where they feed on developing seeds and inject phytotoxic saliva, which causes yield reduction. although leaf responses to herbivory are well studied, no information is available about the regulation of defences in seeds.	2016	26593446
shifts in dynamic regime of an invasive lady beetle are linked to the invasion and insecticidal management of its prey.	the spread and impact of invasive species may vary over time in relation to changes in the species itself, the biological community of which it is part, or external controls on the system. we investigate whether there have been changes in dynamic regimes over the last 20 years of two invasive species in the midwestern united states, the multicolored asian lady beetle harmonia axyridis and the soybean aphid aphis glycines. we show by model selection that after its 1993 invasion into the american ...	2015	26591447
rj4, a gene controlling nodulation specificity in soybeans, encodes a thaumatin-like protein but not the one previously reported.	rj4 is a dominant gene in soybeans (glycine max) that restricts nodulation by many strains of bradyrhizobium elkanii. the soybean-b. elkanii symbiosis has a low nitrogen-fixation efficiency, but b. elkanii strains are highly competitive for nodulation; thus, cultivars harboring an rj4 allele are considered favorable. cloning the rj4 gene is the first step in understanding the molecular basis of rj4-mediated nodulation restriction and facilitates the development of molecular tools for genetic imp ...	2015	26582727
effect of lipid sources with different fatty acid profiles on intake, nutrient digestion and ruminal fermentation of feedlot nellore steers.	the present study was conducted to determine the effect of lipid sources with different fatty acid profiles on nutrient digestion and ruminal fermentation. ten rumen and duodenal fistulated nellore steers (268 body weight±27 kg) were distributed in a duplicated 5×5 latin square. dietary treatments were as follows: without fat (wf), palm oil (po), linseed oil (lo), protected fat (pf; lactoplus), and whole soybeans (ws). the roughage feed was corn silage (600 g/kg on a dry matter [dm] basis) plus ...	0	26580282
impact of radio frequency, microwaving, and high hydrostatic pressure at elevated temperature on the nutritional and antinutritional components in black soybeans.	in this study, the effects of high hydrostatic pressure (hhp) at elevated temperature (60 °c) and 2 dielectric heating (dh) methods (radio frequency [rf], and microwaving [mw]) on the nutritional compositions and removal of antinutritional factors in black soybeans were studied. each treatment caused <2% reduction in protein, and 3.3% to 7.0% decline in total amino acid content. however, the proportion of essential amino acid slightly increased in dh treated samples. the treatment decreased fat ...	2015	26579996
bacteriocins from the rhizosphere microbiome - from an agriculture perspective.	bacteria produce and excrete a versatile and dynamic suit of compounds to defend against microbial competitors and mediate local population dynamics. these include a wide range of broad-spectrum non-ribosomally synthesized antibiotics, lytic enzymes, metabolic by-products, proteinaceous exotoxins, and ribosomally produced antimicrobial peptides (bacteriocins). most bacteria produce at least one bacteriocin. bacteriocins are of interest in the food industry as natural preservatives and in the pro ...	2015	26579159
effect of bacterial root symbiosis and urea as source of nitrogen on performance of soybean plants grown hydroponically for bioregenerative life support systems (blsss).	soybean is traditionally grown in soil, where root symbiosis with bradyrhizobium japonicum can supply nitrogen (n), by means of bacterial fixation of atmospheric n2. nitrogen fertilizers inhibit n-fixing bacteria. however, urea is profitably used in soybean cultivation in soil, where urease enzymes of telluric microbes catalyze the hydrolysis to ammonium, which has a lighter inhibitory effect compared to nitrate. previous researches demonstrated that soybean can be grown hydroponically with reci ...	2015	26579144
an analysis of ozone damage to historical maize and soybean yields in the united states.	numerous controlled experiments find that elevated ground-level ozone concentrations ([o3]) damage crops and reduce yield. there have been no estimates of the actual yield losses in the field in the united states from [o3], even though such estimates would be valuable for projections of future food production and for cost-benefit analyses of reducing ground-level [o3]. regression analysis of historical yield, climate, and [o3] data for the united states were used to determine the loss of product ...	2015	26578785
legumeip 2.0--a platform for the study of gene function and genome evolution in legumes.	the legumeip 2.0 database hosts large-scale genomics and transcriptomics data and provides integrative bioinformatics tools for the study of gene function and evolution in legumes. our recent updates in legumeip 2.0 include gene and protein sequences, gene models and annotations, syntenic regions, protein families and phylogenetic trees for six legume species: medicago truncatula, glycine max (soybean), lotus japonicus, phaseolus vulgaris (common bean), cicer arietinum (chickpea) and cajanus caj ...	2016	26578557
cadmium in soybeans and the relevance to human exposure.		2015	26574099
genome-wide analysis and expression profiling of the pin auxin transporter gene family in soybean (glycine max).	the plant phytohormone auxin controls many aspects of plant growth and development, which largely depends on its uneven distribution in plant tissues. transmembrane proteins of the pin family are auxin efflux facilitators. they play a key role in polar auxin transport and are associated with auxin asymmetrical distribution in plants. pin genes have been characterized in several plant species, while comprehensive analysis of this gene family in soybean has not been reported yet.	2015	26572792
[effects of exogenous α-naphthaleneacetic aid on the antioxidation system in soybean leaves subjected to long-term drought stress during flowering].	two different drought tolerance soybean ( glycine max) varieties (nannong 99-6 and kefeng 1) were used to study the effects of α-naphthaleneacetic acid (naa) on the antioxidation system under long-term drought stress after flowering with pot experiment, which lasted for 110 days at pailou experiment station, nanjing agricultural university, in 2012. the results showed that long-term stress decreased the shoot dry mass significantly, however, increased the level of reactive oxygen species (ros) a ...	2015	26572024
[influence of drought on leaf photosynthetic capacity and root growth of soybeans at grain filling stage].	a drought-resistant soybean cultivar jinda 70 and a drought-sensitive soybean cultivar jindou 26 were taken as test materials. at the grain filling stage, the cultivars were subject to three water treatments including sufficient water supply, light drought stress, and severe drought stress by using pot experiments for research on influence of drought on leaf photosynthetic capacity and root growth of soybeans. the results showed that as the degree of drought stress was aggravated, all of the ind ...	2015	26571660
nopc is a rhizobium-specific type 3 secretion system effector secreted by sinorhizobium (ensifer) fredii hh103.	sinorhizobium (ensifer) fredii hh103 is a broad host-range nitrogen-fixing bacterium able to nodulate many legumes, including soybean. in several rhizobia, root nodulation is influenced by proteins secreted through the type 3 secretion system (t3ss). this specialized secretion apparatus is a common virulence mechanism of many plant and animal pathogenic bacteria that delivers proteins, called effectors, directly into the eukaryotic host cells where they interfere with signal transduction pathway ...	2015	26569401
development of a new vector using soybean yellow common mosaic virus for gene function study or heterologous protein expression in soybeans.	a new vector using soybean yellow common mosaic virus (sycmv) was constructed for gene function study or heterologous protein expression in soybeans. the in vitro transcript with a 5' cap analog m7gpppg from an sycmv full-length infectious vector driven by a t7 promoter infected soybeans (psycmvt7-full). the symptoms observed in the soybeans infected with either the sap from sycmv-infected leaves or psycmvt7-full were indistinguishable, suggesting that the vector exhibits equivalent biological a ...	2016	26569351
has photosynthetic capacity increased with 80 years of soybean breeding? an examination of historical soybean cultivars.	crop biomass production is a function of the efficiencies with which sunlight can be intercepted by the canopy and then converted into biomass. conversion efficiency has been identified as a target for improvement to enhance crop biomass and yield. greater conversion efficiency in modern soybean [glycine max (l.) merr.] cultivars was documented in recent field trials, and this study explored the physiological basis for this observation. in replicated field trials conducted over three successive ...	2016	26565891
an updated review of dietary isoflavones: nutrition, processing, bioavailability and impacts on human health.	isoflavones (genistein, daidzein, and glycitein) are bioactive compounds with mildly estrogenic properties and often referred to as phytoestrogen. these are present in significant quantities (up to 4-5 mg·g(-1) on dry basis) in legumes mainly soybeans, green beans, mung beans. in grains (raw materials) they are present mostly as glycosides, which are poorly absorbed on consumption. thus, soybeans are processed into various food products for digestibility, taste and bioavailability of nutrients a ...	2017	26565435
an integrated biochemical system for nitrate assimilation and nitric oxide detoxification in bradyrhizobium japonicum.	rhizobia are recognized to establish n2-fixing symbiotic interactions with legume plants. bradyrhizobium japonicum, the symbiont of soybeans, can denitrify and grow under free-living conditions with nitrate (no3 (-)) or nitrite (no2 (-)) as sole nitrogen source. unlike related bacteria that assimilate no3 (-), genes encoding the assimilatory no3 (-) reductase (nasc) and no2 (-) reductase (nira) in b. japonicum are located at distinct chromosomal loci. the nasc gene is located with genes encoding ...	2016	26564204
genome-wide association and epistasis studies unravel the genetic architecture of sudden death syndrome resistance in soybean.	soybean [glycine max (l.) merr.] is an economically important crop that is grown worldwide. sudden death syndrome (sds), caused by fusarium virguliforme, is one of the top yield-limiting diseases in soybean. however, the genetic basis of sds resistance, especially with respect to epistatic interactions, is still unclear. to better understand the genetic architecture of soybean sds resistance, genome-wide association and epistasis studies were performed using a population of 214 germplasm accessi ...	2015	26561232
evolution, diversification, and expression of knox proteins in plants.	the knox (knotted1-like homeobox) transcription factors play a pivotal role in leaf and meristem development. the majority of these proteins are characterized by the knox1, knox2, elk, and homeobox domains whereas the proteins of the knatm family contain only the knox domains. we carried out an extensive inventory of these proteins and here report on a total of 394 knox proteins from 48 species. the land plant proteins fall into two classes (i and ii) as previously shown where the class i family ...	2015	26557129
the biomarkers of exposure and effect in agriculture (beea) study: rationale, design, methods, and participant characteristics.	agricultural exposures including pesticides, endotoxin, and allergens have been associated with risk of various cancers and other chronic diseases, although the biological mechanisms underlying these associations are generally unclear. to facilitate future molecular epidemiologic investigations, in 2010 the study of biomarkers of exposure and effect in agriculture (beea) was initiated within the agricultural health study, a large prospective cohort in iowa and north carolina. here the design and ...	2015	26555155
regulation of primary metabolism in response to low oxygen availability as revealed by carbon and nitrogen isotope redistribution.	based on enzyme activity assays and metabolic responses to waterlogging of the legume lotus japonicus, it was previously suggested that, during hypoxia, the tricarboxylic acid cycle switches to a noncyclic operation mode. hypotheses were postulated to explain the alternative metabolic pathways involved, but as yet, a direct analysis of the relative redistribution of label through the corresponding pathways was not made. here, we describe the use of stable isotope-labeling experiments for studyin ...	2016	26553649
rational phosphorus application facilitates the sustainability of the wheat/maize/soybean relay strip intercropping system.	wheat (triticum aestivum l.)/maize (zea mays l.)/soybean (glycine max l.) relay strip intercropping (w/m/s) system is commonly used by the smallholders in the southwest of china. however, little known is how to manage phosphorus (p) to enhance p use efficiency of the w/m/s system and to mitigate p leaching that is a major source of pollution. field experiments were carried out in 2011, 2012, and 2013 to test the impact of five p application rates on yield and p use efficiency of the w/m/s system ...	2015	26540207
the mycobiota of air inside and outside the meju fermentation room and the origin of meju fungi.	the fungi on meju are known to play an important role as degrader of macromolecule of soybeans. in order to elucidate the origin of fungi on traditional meju, mycobiota of the air both inside and outside traditional meju fermentation rooms was examined. from 11 samples of air collected from inside and outside of 7 meju fermentation rooms, 37 genera and 90 species of fungi were identified. in outside air of the fermentation room, cladosporium sp. and cladosporium cladosporioides were the dominant ...	2015	26539041
factors underlying genotypic differences in the induction of photosynthesis in soybean [glycine max (l.) merr].	crop leaves are subject to continually changing light levels in the field. photosynthetic efficiency of a crop canopy and productivity will depend significantly on how quickly a leaf can acclimate to a change. one measure of speed of response is the rate of photosynthesis increase toward its steady state on transition from low to high light. this rate was measured for seven genotypes of soybean [glycine max (l.) merr.]. after 10 min of illumination, cultivar 'ua4805' (ua) had achieved a leaf pho ...	2016	26538465
co-expression of g2-epsps and glyphosate acetyltransferase gat genes conferring high tolerance to glyphosate in soybean.	glyphosate is a widely used non-selective herbicide with broad spectrum of weed control around the world. at present, most of the commercial glyphosate tolerant soybeans utilize glyphosate tolerant gene cp4-epsps or glyphosate acetyltransferase gene gat separately. in this study, both glyphosate tolerant gene g2-epsps and glyphosate degraded gene gat were co-transferred into soybean and transgenic plants showed high tolerance to glyphosate. molecular analysis including pcr, sothern blot, qrt-pcr ...	2015	26528311
sinorhizobium fredii hh103 bacteroids are not terminally differentiated and show altered o-antigen in nodules of the inverted repeat-lacking clade legume glycyrrhiza uralensis.	in rhizobial species that nodulate inverted repeat-lacking clade (irlc) legumes, such as the interaction between sinorhizobium meliloti and medicago, bacteroid differentiation is driven by an endoreduplication event that is induced by host nodule-specific cysteine rich (ncr) antimicrobial peptides and requires the participation of the bacterial protein baca. we have studied bacteroid differentiation of sinorhizobium fredii hh103 in three host plants: glycine max, cajanus cajan and the irlc legum ...	2016	26521863
genome-wide association study, genomic prediction and marker-assisted selection for seed weight in soybean (glycine max).	twenty-two loci for soybean sw and candidate genes conditioning seed development were identified; and prediction accuracies of gs and mas were estimated through cross-validation and validation with unrelated populations. soybean (glycine max) is a major crop for plant protein and oil production, and seed weight (sw) is important for yield and quality in food/vegetable uses of soybean. however, our knowledge of genes controlling sw remains limited. to better understand the molecular mechanism und ...	2016	26518570
a novel cis-acting element in the gmerf3 promoter contributes to inducible gene expression in soybean and tobacco after wounding.	using in silico and functional analyses, we cloned and validated the expression profile of an inducible soybean promoter (gmerf3) along with its novel wound-induced and delayed expression (wide) element. promoters and their contributing promoter elements are the main regulators of gene expression at the transcriptional level. although the ethylene response factor (erf) gene family is one of the most well-studied stress-responsive gene families in plants, their promoter regions have received litt ...	2016	26518427
association of processed meat intake with hypertension risk in hemodialysis patients: a cross-sectional study.	in this cross-sectional study, we hypothesized that hemodialysis patients consuming greater processed meat is associated with hypertension risk, which can be partly explained by the high sodium content in processed meat. from september 2013 to may 2014, one hundred and four patients requiring chronic hemodialysis treatment were recruited from hemodialysis centers. data on systolic blood pressure and diastolic blood pressure before receiving dialysis, and 3-day dietary records of the recruited pa ...	2015	26517837
proteomic analysis of the relationship between metabolism and nonhost resistance in soybean exposed to bipolaris maydis.	nonhost resistance (nhr) pertains to the most common form of plant resistance against pathogenic microorganisms of other species. bipolaris maydis is a non-adapted pathogen affecting soybeans, particularly of maize/soybean intercropping systems. however, no experimental evidence has described the immune response of soybeans against b. maydis. to elucidate the molecular mechanism underlying nhr in soybeans, proteomics analysis based on two-dimensional polyacrylamide gel electrophoresis (2-de) was ...	2015	26513657
soybean fruit development and set at the node level under combined photoperiod and radiation conditions.	in soybean, long days during post-flowering increase seed number. this positive photoperiodic effect on seed number has been previously associated with increments in the amount of radiation accumulated during the crop cycle because long days extend the duration of the crop cycle. however, evidence of intra-nodal processes independent of the availability of assimilates suggests that photoperiodic effects at the node level might also contribute to pod set. this work aims to identify the main mecha ...	2016	26512057
effect of planting date and maturity group on soybean yield response to injury by megacopta cribraria (hemiptera: plataspidae).	the kudzu bug, megacopta cribraria (f.), is an invasive member of the family plataspidae originating from asia. since its discovery in georgia in 2009, its distribution has increased to 13 southern and eastern states. in the united states, m. cribraria is bivoltine and has two primary developmental hosts, kudzu and soybean. here, we evaluated the yield response of soybean to m. cribraria feeding injury in relation to planting date and soybean maturity group. the study contained four replicated t ...	2016	26511984
gmcol1a and gmcol1b function as flowering repressors in soybean under long-day conditions.	constans (co) has a central role in the photoperiod response mechanism in arabidopsis. however, the functions of legume co genes in controlling flowering remain unknown. here, we analyze the expression patterns of e1, e2 and gmcol1a/1b using near-isogenic lines (nils), and we further analyze flowering-related genes in gmcol1b mutants and gmcol1a-overexpressing plants. our data showed that both e3 and e4 up-regulate e1 expression, with the effect of e3 on e1 being greater than the effect of e4 on ...	2015	26508522
performance of hybrid progeny formed between genetically modified herbicide-tolerant soybean and its wild ancestor.	gene flow from genetically modified (gm) crops to wild relatives might affect the evolutionary dynamics of weedy populations and result in the persistence of escaped genes. to examine the effects of this gene flow, the growth of f1 hybrids that were formed by pollinating wild soybean (glycine soja) with glyphosate-tolerant gm soybean (g. max) or its non-gm counterpart was examined in a greenhouse. the wild soybean was collected from two geographical populations in china. the performance of the w ...	2015	26507568
whole-genome sequencing and comparative genome analysis of bacillus subtilis strains isolated from non-salted fermented soybean foods.	bacillus subtilis is the main component in the fermentation of soybeans. to investigate the genetics of the soybean-fermenting b. subtilis strains and its relationship with the productivity of extracellular poly-γ-glutamic acid (γpga), we sequenced the whole genome of eight b. subtilis stains isolated from non-salted fermented soybean foods in southeast asia. assembled nucleotide sequences were compared with those of a natto (fermented soybean food) starter strain b. subtilis best195 and the lab ...	2015	26505996
analysis of the community compositions of rhizosphere fungi in soybeans continuous cropping fields.	we used rhizosphere soil sampled from one field during zero year and two years of continuous cropping of high-protein soybean to analyze the taxonomic community compositions of fungi during periods of high-incidence of root rot. our objectives were to identify the dominant pathogens in order to provide a theoretical basis for the study of pathogenesis as well as control tactics for soybean root rot induced by continuous cropping. a total of 17,801 modified internal transcribed spacer (its) seque ...	2015	26505311
medicago truncatula and glycine max: different drought tolerance and similar local response of the root nodule proteome.	legume crops present important agronomical and environmental advantages mainly due to their capacity to reduce atmospheric n2 to ammonium via symbiotic nitrogen fixation (snf). this process is very sensitive to abiotic stresses such as drought, but the mechanism underlying this response is not fully understood. the goal of the current work is to compare the drought response of two legumes with high economic impact and research importance, medicago truncatula and glycine max, by characterizing th ...	2015	26503705
phosphorylation-dependent regulation of g-protein cycle during nodule formation in soybean.	signaling pathways mediated by heterotrimeric g-protein complexes comprising gα, gβ, and gγ subunits and their regulatory rgs (regulator of g-protein signaling) protein are conserved in all eukaryotes. we have shown that the specific gβ and gγ proteins of a soybean (glycine max) heterotrimeric g-protein complex are involved in regulation of nodulation. we now demonstrate the role of nod factor receptor 1 (nfr1)-mediated phosphorylation in regulation of the g-protein cycle during nodulation in so ...	2015	26498905
chromohalobacter is a causing agent for the production of organic acids and putrescine during fermentation of ganjang, a korean traditional soy sauce.	ganjang, a korean traditional fermented soy sauce, is prepared by soaking doenjang-meju (fermented soybeans) in approximately 20% (w/v) solar salt solution. the metabolites and bacterial communities during ganjang fermentation were simultaneously investigated to gain a better understanding of the roles of the microbial population. the bacterial community analysis based on denaturing gradient gel electrophoresis of 16s rrna gene sequences showed that initially, the genus cobetia was predominant ( ...	2015	26495904
draft genome sequence of bradyrhizobium japonicum is-1, which is incompatible with rj2 genotype soybeans.	we report the draft genome sequence of bradyrhizobium japonicum is-1, which is incompatible with rj2 genotype soybeans. the estimated genome size of this strain is 8.9 mb. genome sequence information of this strain will help to identify a causal gene for this incompatibility.	2015	26494666
qtl analysis of seed-flooding tolerance in soybean (glycine max [l.] merr.).	in soybean (glycine max [l.] merr.), varieties with seed-flooding tolerance at the geminating stage are desirable for breeding in countries with much rainfall at sowing time. our study revealed great intervarietal variation in seed-flooding tolerance as evaluated by germination rate (gr) and normal seedling rate (ns). pigmented seed coat and small seed weight tended to give a positive effect on seed-flooding tolerance. subsequently, qtl analysis of gr and ns were performed and a total of four qt ...	2009	26493141
assessment of fatty acid profile and seed mineral nutrients of two soybean (glycine max l.) cultivars under elevated ultraviolet-b: role of ros, pigments and antioxidants.	current scenarios under global climate change envisage a considerable increase in ultraviolet b (uv-b) radiation in near future which may affect the productivity and yield quality of major agricultural crops. present investigation was conducted to examine various defense strategies adopted against elevated uv-b (ambient + 7.2 kj m-(2) day-(1) ) and their impact on seed nutrients, content and quality of oil including fatty acid profile of two soybean cultivars (js-335 and ps-1042). elevated uv-b ...	2016	26489397
metabolite profiling of soybean seed extracts from near-isogenic low and normal phytate lines using orthogonal separation strategies.	untargeted metabolomic profiling using liquid chromatography-mass spectrometry (lc-ms) was applied to lipid-depleted methanolic extracts of soybean seeds utilizing orthogonal chromatographic separations (reversed-phase and hydrophilic interaction) in both positive and negative ionization modes. four near-isogenic lines (nils) differing in mutations for two genes encoding highly homologous multidrug resistant proteins (mrps) were evaluated. the double mutant exhibited a low phytate phenotype, whe ...	2015	26487475
additivity of the stabilization effect of single amino acid substitutions in triple mutants of recombinant formate dehydrogenase from the soybean glycine max.	recently, we demonstrated that the amino acid substitutions ala267met and ala267met/ile272val (alekseeva et al., biochemistry, 2012), phe290asp, phe290asn and phe290ser (alekseeva et al., prot. eng. des. select, 2012) in recombinant formate dehydrogenase from soya glycine max (soyfdh) lead to a significant (up to 30-100 times) increase in the thermal stability of the enzyme. the substitutions phe290asp, phe290asn and phe290ser were introduced into double mutant soyfdh ala267met/ile272val by site ...	2015	26483960
development of indel markers for genetic mapping based on whole genome resequencing in soybean.	soybean [glycine max (l.) merrill] is an important crop worldwide. in this study, a chinese local soybean cultivar, hedou 12, was resequenced by next generation sequencing technology to develop insertion/deletion (indel) markers for genetic mapping. 49,276 indel polymorphisms and 242,059 single nucleotide polymorphisms were detected between hedou 12 and the williams 82 reference sequence. of these, 243 candidate indel markers ranging from 5-50 bp in length were chosen for validation, and 165 (68 ...	2015	26483012
soybeans or soybean products consumption and depressive symptoms in older residents in rural northeast china: a cross-sectional study.	depression is one of the most common mental disorders among elderly subjects. the purpose of this study is to estimate the prevalence of depressive symptoms and the association between soybeans consumption and depressive symptoms among older residents in rural northeast china.	2015	26482689