Publications

expression of the plum pox coat protein gene in transgenic nicotiana tabacum plants.	plant expression vector pbi 121 containing the gene encoding coat protein of plum pox virus of the skierniewice isolate (cp ppv-s) was prepared (clone pcm1). the construct was used for transformation of nicotiana tabacum plants using an agrobacterium tumefaciens based system. about 82% of kanamycin resistant plant lines contained a transgene (the sequence of cp ppv-s) but only 81% of them actively expressed the ppv-s coat protein gene as measured by rt-pcr.	1995	7653168
randomly amplified polymorphic dna analysis of xylella fastidiosa pierce's disease and oak leaf scorch pathotypes.	randomly amplified polymorphic dna analysis was conducted with 14 primers to 17 strains of xylella fastidiosa. there was a high degree of similarity among the seven pierce's disease (pd) strains (sxy > 0.93) and the seven oak leaf scorch (ols) strains (sxy > 0.96). however, the two groups were different, with a similarity index of 0.67, confirming the presence of a pd dna cluster and suggesting the presence of a new ols cluster. the control plum leaf scald strains (two strains) together with the ...	1995	7646005
genetic relationships among strains of xylella fastidiosa from rapd-pcr data.	genetic relationships among 11 xylella fastidiosa strains isolated from mulberry, almond, ragweed, grape, plum, elm, and citrus were determined by random amplified polymorphic dna (rapd). twenty-two 10-base primers amplified a total of 77 discrete polymorphic bands. phenetic analysis based on a similarity matrix corresponded well with previous reports on x. fastidiosa rflp-based similarity relationships, indicating that rapd-pcr amplification products can be used as a reliable indicator of genet ...	1995	7606188
transmission by aphids of a naturally non-transmissible plum pox virus isolate with the aid of potato virus y helper component.	two spanish plum pox virus (ppv) isolates, 5.15 and 3.3, were used in transmission experiments involving the aphid vector myzus persicae, with woody and herbaceous host plants. these isolates differ in the size of their coat protein (cp) and sequence analysis revealed that isolate 3.3 has a 15 amino acid deletion near the n terminus of the cp, affecting the same positions as in a previously reported non-aphid-transmissible ppv isolate from germany. aphid transmission experiments showed that isol ...	1995	7561767
rna helicase activity of the plum pox potyvirus ci protein expressed in escherichia coli. mapping of an rna binding domain.	the plum pox potyvirus (ppv) cylindrical inclusion (ci) protein fused to the maltose binding protein (mbp) has been synthesized in escherichia coli and purified by affinity chromatography in amylose resin. in the absence of any other viral factors, the fusion product had ntpase, rna binding and rna helicase activities. these in vitro activities were not affected by removal of the last 103 amino acids of the ci protein. however, other deletions in the c-terminal part of the protein, although leav ...	1995	7538661
production and characterization of monoclonal antibodies to plum pox virus and their use in differentiation of mediterranean isolates.	monoclonal antibodies (mabs) specific to plum pox virus (ppv) were prepared by fusing myeloma cell lines to spleen cells of mice immunized with purified virus, including virus prepared with protease inhibitors to preserve the integrity of the coat protein (cp). the characterized mabs could be used in elisa to differentiate several mediterranean ppv isolates differing in their geographical origin and cp size. at least seven antigenic sites could be established based on the recognition pattern and ...	1994	7526822
properties of the active plum pox potyvirus rna polymerase complex in defined glycerol gradient fractions.	as a first step in the study of the replication of plum pox virus (ppv) rna, an in vitro virus-specific rna polymerase activity was characterized in a crude membrane extract (martin and garcia, 1991). in this study, we report the fractionation of the crude membrane extract by centrifugation in glycerol gradients. the sedimentation properties after different treatments of the crude extract and its insensitivity to micrococcal nuclease treatment suggest that the rna polymerase activity was localiz ...	1995	7483826
[clinical aspects of the prognosis of skull and brain injuries. a study of 108 cases].	an assessment is made of the extent to which age and the degree an seriousness of the initial neurological picture influenced prognosis in a series of 108 subjects with brain injuries treated in the siena hospital intensive care unit between 1974 and 1977. neurological disease was classified according to posner and plum. mortality was 46.2%. in most cases, age tended to exacerbate the neurological syndrome from the outset. it also facilitated the appearance of respiratory sepsis, which aggravate ...	1980	7465086
[two cases of intraventricular meningiomas in children (author's transl)].	for several months a 14-year-old girl had suffered from increased intracranial pressure which in spite of extensive calcification in the fourth ventricle had not been recognized. during the operation a plum-sized tumour was found free moving in the fourth ventricle. being hard as bone it had to be extracted in toto. the histological examination showed a highly calcified endotheliomatous meningioma. recovery without complications. for seven years a 12-year-old boy had a tumour in the third ventri ...	1981	7343847
free sugars and sorbitol in fruits--a complication from the literature.	the glucose, fructose, sucrose, and sorbitol content of apple, pear, plum, cherry, grape, strawberry, raspberry, blackberry, and peak fruit was compiled from the literature; their range, mean, standard deviation, and percent coefficient of variance were calculated. the individual fruits have characteristic patterns relating to their sorbitol content, glucose: fructose ratio, and sucrose content which are influenced to only a small degree by variety, season, or geographic origin. processing in ma ...	1981	7204315
[survey on antibodies against human influenza viruses in the sera of "plum-flower" deer].		1982	7185425
detection of adulteration in blackberry juice concentrates and wines.	adulteration of blackberry juice concentrates and wines with juice of sorbitol-containing fruits was detected by determining carbohydrates by high performance liquid chromatography, gas-liquid chromatography, and thin layer chromatography. sorbitol is not fermented by yeast and can be detected in wines made from blackberry juice concentrates that contain sorbitol. high levels of sorbitol and quinic and malic acids suggest that an imported blackberry concentrate may have been adulterated with plu ...	1982	7174584
[forme fruste of progressive lipodystrophy or multiple symmetrical surface lipomatosis?].	it is reported on a 44-year-old patient who has a peculiar form of the disturbance of the distribution of fatty tissue. apart from clear superficial hypertrophies at upper arms, shoulders, thorax and hypogastrium as well as plum-sized nodes at the left femor and retroauricularly discrete atrophies are found in the face, at forearms and lower legs. in very inconspicuous paraclinical findings special forms of the progressive lipodystrophy and of the indolent symmetrical lipomatosis are discussed.	1982	7080563
some considerations on the central regulation of respiration and its disturbances.	the effect of increased intracranial pressure on the respiration pattern was studied in cats. it appeared that the different patterns described by plum and brown blend into each other under certain conditions.	1982	7072554
intestinal metaplasia in japan: association with diet.	of 1,749 volunteers in a screening program for gastric cancer in japan, 206 men and 181 women received a systematic gastroscopic examination and responded to a dietary questionnaire estimating their quantitative intake of 33 food items during the past weeks. when diet was correlated with intestinal metaplasia (a precursor lesion of gastric carcinoma) found in gastroscopic biopsies, it was observed that more dried fish consumption and less vitamin a intake increased the extent of intestinal metap ...	1982	6950167
regional cerebral glucose utilization measured with the 2--[ 14c] deoxyglucose technique: its use in mapping functional activity in the nervous system.	the results of studies with the [ 14c] deoxyglucose technique unequivocally establish that functional activity in specific components of the central nervous system is, as in other tissues, closely coupled to the local rate of energy metabolism. stimulation of functional activity increases the local rate of glucose utilization; reduced functional activity depresses it. these changes are so profound that they can be visualized directly in autoradiographic representations of local tissue concentrat ...	1980	6945030
[4 simultaneous cases of methanol poisoning caused by home-made plum brandy].		1983	6845480
the estimation of epistasis in components of fitness in experimental populations of drosophila melanogaster ii. assessment of meiotic drive, viability, fecundity and sexual selection.	an analysis of fitness components was performed on laboratory populations of drosophila melanogaster bearing curly and plum marked second chromosome inversions with two genetic backgrounds. selective differences between curly, plum, curly-plum, and wild phenotypes were partitioned into meiotic drive, viability, fecundity and mating success. although no significant meiotic drive was detected, there were significant differences between genotypes in the other three components. interlocus interactio ...	1981	6792163
the estimation of epistasis in components of fitness in experimental populations of drosophila melanogaster i. a two-stage maximum likelihood model.	laboratory populations of drosophila melanogaster bearing the curly and plum marked second chromosome inversions were observed in selection experiments for ten discrete generations. maximum likelihood estimates of the relative fitnesses of curly, plum, curly-plum, and wild phenotypes were obtained from selection trajectories. using these estimates, measures of multiplicative and additive epistasis were calculated. these were partitioned into pre-sampling and post-sampling components, and both we ...	1981	6792162
[a gouty tophus in the temporomandibular joint and on the eustachian tube].	a 76-year old female patient was suffering from a right-sided conduction-type hearing loss due to a very painful tumour in the anterior parotid region. x-ray films showed a partial destruction of the right temporomandibular joint. since the patient had undergone a left-sided mastectomy for breast carcinoma two years previously, an operation was performed under the suspicion of a metastatic lesion. a plum-sized gouty tophus was found adjacent to the temporomandibular joint. the head of the mandib ...	1983	6672499
nutrient composition of stone fruit (prunus spp.) cultivars: apricot, cherry, nectarine, peach and plum.		1983	6664077
neurodermatitis treated by plum-blossom needle.		1984	6570497
observation on 52 cases of paralysis of common peroneal nerve treated by acupuncture and plum-blossom needling.		1984	6567734
plum-blossom needle therapy in concomitant strabismus. a report of 103 cases.		1984	6565887
[classifications and therapeutic indications in spontaneous intracerebral hematomas in the absence of any vascular malformation. preliminary study apropos of 65 cases].	ct scan and i.c.p. monitoring help us in management of spontaneous intracerebral haemorrhage to decide if surgical evacuation is necessary or not. the various opinions expressed in literature seem to depend on the lack of correlations between clinical state, ct scan and i.c.p. monitoring. three groups of patients were studied: group i: patients with ingravescent coma during the first three days. their natural history is death. group 2: patients with disturbances of consciousness which appear, pe ...	1984	6462319
rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs.	virus particles morphologically similar to caliciviruses and rotaviruses were detected by electron microscopy (em) in the intestinal contents of a 27-day-old diarrheic nursing pig. a third small spherical 23-nm virus-like particle was also observed. calicivirus-like particles averaged 33 nm in diameter. similar to rotaviruses, rotavirus-like particles were present as single-capsid 55-nm forms or double-capsid 70-nm particles. most gnotobiotic pigs orally exposed to samples containing these three ...	1980	6252238
immunogenic variation among the so-called lc strains of mycoplasma mycoides subspecies mycoides.	much evidence of immunogenic heterogeneity among the lc strains of mycoplasma mycoides ssp. mycoides emerged from cross-immunization and -hyper-immunization experiments in mice in which three lc strains (vom/plum island, 74/2488, and mankefår 2833) were used for challenge purposes. all heterologous lc-strain vaccines cross-immunized against the three challenge strains, but protection was usually only 'partial', i.e. significantly less than that given by homologous vaccine. cross-hyperimmunizatio ...	1983	6190898
the method of pollination with a pollen mixture to obtain interspecific hybrids of plum and cherry.		1965	5883035
laboratory mating behavior of the plum curculio.		1969	5778308
[localization and migration of the sarka virus (plum pox virus)].		1969	5396079
[properties and serology of the sarka virus of the plum].		1969	5396074
tannins in black-plum (syzygium cumini l.) seeds.		1972	5085637
presence and persistence of foot-and-mouth disease virus in bovine skin.	gailiunas, peter (plum island animal disease laboratory, greenport, n. y.), and george e. cottral. presence and persistence of foot-and-mouth disease virus in bovine skin. j. bacteriol. 91:2333-2338. 1966.-this study established that the seven known antigenic types of foot-and-mouth disease virus (fmdv) have consistent affinity to all areas of bovine skin, even though gross cutaneous lesions usually are found only in the pedal area. considerable amounts of fmdv were present in skin of 13 differe ...	1966	4287587
beware of the 'plum'--ureterocele. case report.		1985	4041404
[contamination of food products with patulin].	based on the method for patulin determination designed by the authors the rate and level of contamination of fruit, vegetables and foods manufactured from them (juices, purees, jams) with this mycotoxin were studied. patulin was found in 8 out of 160 samples of fruit and vegetables, the highest concentration of patulin was identified in the berries of sea buckthorn (up to 54 000 micrograms/kg). patulin was found in 28 out of 185 samples of foods manufactured from fruit. the concentration of patu ...	1985	3984287
coupled instability of two x-linked genes in drosophila mauritiana: germinal and somatic mutability.	a highly unstable allele has been isolated at the white locus of drosophila mauritiana, a sibling species of d. melanogaster. this allele, white-peach (wpch), mutates spontaneously in males and females to give both wild-type and bleached-white derivatives. the mutation frequency is about 10(-3) mutations/generation. there is no evidence for clustering among mutant progeny, and phenotypically wpch flies with mosaic patches of wild-type tissue in the eyes are frequently recovered. another x-linked ...	1985	3928432
cutaneous xanthomas associated with chronic myelomonocytic leukemia.	monocytic leukemia may be associated with specific cutaneous changes in about a third of all cases, most characteristically the plum-colored nodule. nonspecific cutaneous xanthomas have occasionally been reported with a variety of leukemias, mostly in the pediatric literature, but the first report (to our knowledge) of acute myelomonocytic leukemia presenting with specific cutaneous xanthomatous lesions appeared in 1982. herein is reported a case of chronic myelomonocytic leukemia presenting wit ...	1985	3862364
neuropsychological sequelae after head injury with mesencephalic coma in childhood.	eleven children aging between 4 years 11 months and 12 years 6 months were examined periodically over five years following closed head injury with mesencephalic coma (plum and posner's definition). it was found that although transient sequelae (neurological and cognitive) on coma resolution were severe, long-term recovery was generally good. the subjects had drug, neurological and psychological treatment during the 2-5-years period and showed apparently better recovery than reported in literatur ...	1985	3841915
a proposed method for using a reimbursement moratorium to encourage recruitment for a randomized study of carotid endarterectomy.	as indicated by barnett, plum and walton, a definitive clinical trial to resolve the issue of the effectiveness of carotid endarterectomy is clearly needed. a proposal to foster such a trial, through a moratorium on third-party reimbursement other than for randomized patients, has herein been presented.	1986	3810740
[recurrent cerebral emboli in nonbacterial thrombotic endocarditis].	a 28-year-old woman developed thrombophlebitis migrans and right hemiparesis with motor aphasia. chest x-ray revealed a plum-sized central infiltrate in the right lower lobe of the lung. transbronchial lung biopsy showed bronchoalveolar carcinoma. subsequently recurrent cerebral infarcts developed from which the patient died. autopsy revealed nonbacterial thrombotic endocarditis as the cause of the cerebral infarcts. this form of endocarditis is characterized by the parallel occurrence of arteri ...	1987	3678075
[a new criminologic procedure: study of l-cystine-aminopeptidase activity for the detection of seminal stains].	l-cystine-aminopeptidase (cap) enzyme cleaves l-cystine-di-beta-naphthylamide substrate to beta-naphthylamine. the final product of this process is a violet azostain. cap enzyme determination was developed for criminological procedures. cap shows almost 100 times greater activity in human semen than in other body fluids. samples taken at different time intervals (1 week, 1-3-6 months, 1 year, and 5-10 years) were investigated; they were dried on cloth and filter paper and then stored at room tem ...	1987	3660956
coma.	the preceding discussion is an attempt to stress anatomy in the diagnosis of coma. by localizing the offending lesion, the physician can apply the appropriate diagnostic and therapeutic measures more adeptly. because physicians are also frequently asked to prognosticate, i have tried to summarize the work of plum and posner in their study of the outcome of patients presenting in coma. the primary care physician is encouraged to plan at least for the basics in case he or she is faced with a comat ...	1986	3633597
lead poisoning in canada geese on plum island, massachusetts.	during december 1983 and early january 1984, about 200 canada geese (branta canadensis) died of lead poisoning at parker river national wildlife refuge on plum island, massachusetts. in an effort to determine the source of lead, 100 bottom samples were taken from a refuge impoundment where much of the mortality/morbidity occurred. an average of 157,150 pellets/ha was found with a range of 64,582 to 322,910 pellets/ha. water levels in this impoundment were low when canada geese arrived, making sh ...	1987	3625903
a simple gas chromatography method for the determination of ethylcarbamate in spirits.	ethylcarbamate is a toxic component of certain spirits. for its determination, the spirit is concentrated to one-fifth of its volume, extracted with ethylacetate and analysed by capillary gas chromatography (gc) with flame ionization detection (fid). the method saves time and material and has proved to be a reliable test in different laboratories. the ethylcarbamate concentrations in 170 samples of swiss origin are presented. the mean values were 1.0 mg/l in cherry brandies, 1.5 mg/l in plum bra ...	1987	3617935
[plum pits in the terminal ileum].	we report on a case of an inflammatory conglomerate tumour proximal to the ileocaecal valve which resulted from ingested plum stones and after years led to abundant intestinal bleeding.	1987	3616428
a basis for estimation of consumption: literature values for selected food volatiles. part ii.	in this paper we present a compilation of quantitative literature data on volatile compounds in 15 food items including some brandies, meats, oils as well as vegetables, vinegar and potatoes. levels of the volatile compounds identified (approximately 900) in this group of food items are generally in the ppm range. carboxylic acids were present in much higher levels in plum brandy, vinegar, lamb and mutton (heated), whereas alcohols, esters and carbonyls aldehydes are particularly abundant in bra ...	1987	3595923
the effects of fruit juices and fruits on the absorption of iron from a rice meal.	the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ...	1987	3593665
[results of skin tests with pollen extracts of fruit trees in patients with pollen allergy].	as there have recently been increasing publications on the correlation between tree pollen allergy and fruit allergy, we asked ourselves wether this might be caused by the parallel pollen times of fagales trees and rosales trees in our central european climate. accordingly, we tested 92 patients positively suffering from fagales tree pollen allergy with the following rosales tree pollen extracts: apple, cherry, plum, apricote, almande. 16 out of these patients showed reactions to fagales pollen ...	1987	3577285
imps (intact months of patient survival): an analysis of the results of carotid endarterectomy.	the literature on carotid surgery for lesions appropriate to prior episodes of ischemia has been reviewed. only one randomized study and six non-controlled reports give useful data (this despite more than thirty years of surgical activity in this field). when analyzed by the imps (intact months of patient survival) criterion, the randomized study failed to show benefit from surgery. this failure can be attributed to a high (35%) operative stroke and death rate. that sufficiently low operative st ...	1986	3544350
efficacy of human chorionic gonadotrophin and gonadotrophin releasing hormone for hastening ovulation in thoroughbred mares.	plasma progesterone levels were measured daily to determine the accuracy of diagnosing ovulation by rectal palpation carried out every other day; 81.5 per cent mares injected with human chorionic gonadotrophin showed increases of progesterone more than 1 ng/ml by 72 h after injection compared with 65 per cent of mares injected with gonadotrophin releasing hormone (gnrh) or saline. mating at ovulation achieved a 74 per cent pregnancy rate in mares given hcg compared with 50 per cent given gnrh an ...	1986	3542528
radiation preservation of foods of plant origin. part v. temperate fruits: pome fruits, stone fruits, and berries.	the current status of research on the application of ionizing radiation for improving the storage of temperate fruits, i.e., apple, pear, peach, nectarine, apricot, cherry, plum, strawberry, bilberry, cranberry, raspberry, and black currant, is reviewed. changes in fruit metabolism, chemical composition, texture, and organoleptic quality attributes are discussed with reference to the irradiation dose. the feasibility of using radiation either alone or in conjunction with heat treatment, refriger ...	1986	3536313
synthetic oligonucleotide hybridization probes to diagnose hop stunt viroid strains and citrus exocortis viroid.	four species of synthetic oligonucleotide probes for the diagnosis of hop stunt viroid (hsv) and citrus exocortis viroid (cev) were devised. probe hsv-1 detected all the members of hsv group, such as hsv-hop, hsv-grapevine, hsv-cucumber, hsv-citrus and a viroid-like rna isolated from plum trees affected by plum dapple fruit disease. probe hsv-2 discriminated hsv-grapevine from the other members of hsv group. hsv-hop and hsv-grapevine consist of the same numbers of nucleotides, with only one nucl ...	1988	3366851
angiolymphoid hyperplasia of the head and neck.	angiolymphoid hyperplasia is a rare benign condition affecting the skin and subcutaneous tissue of the head and neck. superficial (intradermal) lesions frequently affect the external ear and such a case presenting to an otolaryngologist is described. clinically the disease is characterized by single or multiple plum-coloured nodules or plaques which are often itchy and may bleed with mild trauma. histologically the lesions are composed of proliferating capillary vessels associated with a heavy i ...	1988	3343552
[sonography in the early detection of non-palpable second testicular tumors: a prospective study].	as part of the tumor aftercare service afforded by our policlinic, 91 patients were examined one to six times in 18 months following radical orchiectomy for testicular germ cell tumors. the period of observation averaged 35 months (3-147) after the contralateral removal of the testicle. in addition to the usual routine checks, sonography of the residual testicle also formed part of the established followup protocol. among these patients sonography identified in residual testicles 3 testicular tu ...	1987	3324447
inheritance of phosphogluconate and xanthine dehydrogenases in aedes (finlaya) togoi.	phosphogluconate dehydrogenase (pgd) and xanthine dehydrogenase (xdh) were genetically studied by agar gel electrophoresis in the mosquito aedes togoi. adult homogenates displayed banding patterns with one zone of activity of either enzyme. eight backcrosses were conducted to map the two loci, pgd and xdh, to linkage group iii with the following arrangement: pgd--(ca. 15 map units)--bl (bleached pupa)--(ca.5)--y (yellow larva)--(38.0 +/- 1.7)--pm (plum eye)--(6.1 +/- 0.9)--xdh. all pgd and xdh l ...	1988	3225566
case of the month. a geriatric plum.		1988	3208015
incarcerated richter's hernia after laparoscopy: a case report.	an unusual case of richter's hernia on the 5th day after laparoscopy is presented. the complaints simulated a postoperative hematoma, but they became so intense that the expansion was surgically explored: a conservative procedure on the loop of small bowel was possible. to prevent this complication after laparoscopy careful shaking of the abdominal wall at removal of the instruments is necessary.	1988	2968930
[cultivation and production technics of the dark plum (prunus mume)].		1985	2938794
the effect of ethanol upon early development in mice and rats. viii. the effect of chronic consumption of some beverages upon preimplantation development in rats.	the effects of chronic consumption of some beverages (plum-brandy 24% and cognac 20%) upon preimplantation development in rats were studied. the control of possible effects was performed on day 5 by usual flushing, examination and photographying of oviductal and uterine embryos. in order to evaluate the effect of the beverage applied, the following criteria were used: mean litter size, migration of the embryos from the oviduct to the uterus, the developmental stage attained by the pre-implantati ...	1985	2934620
chronic vegetative state after severe head injury: clinical study; electrophysiological investigations and ct scan in 15 cases.	fifteen cases of chronic vegetative state (cvs), following severe head injury and lasting for two years or more, are reported. vegetative state, in most instances after a period of coma, consists of a return of wakefulness accompanied by an apparent total lack of higher mental activity. a protracted period of vegetative state has been chosen to ensure that the possibility of further recovery could virtually be excluded. the term of cvs could therefore be reasonably used to designate these cases. ...	1989	2812420
the genome-linked protein and 5' end rna sequence of plum pox potyvirus.	the infectivity of plum pox potyvirus (ppv) rna was decreased by treatment with proteases. ribonuclease digestion of iodinated ppv rna yielded material which had an electrophoretic mobility corresponding to mr 22,000. this protein presumably corresponds to the protease-sensitive structure needed for infectivity. a protein-linked rnase t1-resistant oligonucleotide, 38 nucleotides long, was sequenced and shown to correspond to the 5' terminus of the rna by sequence comparison to the rnas of two ot ...	1989	2794981
the complete nucleotide sequence of plum pox potyvirus rna.	the complete nucleotide sequence of the plum pox virus (ppv) rna genome has been determined. the rna sequence is 9786 nucleotides in length, excluding the 3'-terminal poly(a) tail. an aug triplet at position 147-149 was assigned as the initiation codon for the translation of the genome size viral polyprotein which would consist of 3140 amino acid residues. the nucleotide sequence of the non-coding regions and the predicted amino acid sequence of the polyprotein of ppv were compared with those pr ...	1989	2773595
plum-colored nodules in a patient with chronic myelogenous leukemia. leukemia cutis in blast phase of cml.		1989	2757412
hop stunt viroid strains from dapple fruit disease of plum and peach in japan.	viroids have been isolated from plum trees (prunus salicina lindley) affected with plum dapple fruit disease and from peach trees (prunus persica batsch) showing dapple fruit symptoms. the viroids were inoculated mechanically to cucurbitaceous plants, in which symptoms typical of hop stunt viroid (hsv) infection appeared. the complete nucleotide sequences of an isolate from plum and an isolate from peach (af isolate) were shown to be identical, consisting of 297 nucleotides with a 93.6% sequence ...	1989	2732717
the complete nucleotide sequence of plum pox virus rna.	the complete nucleotide sequence of the rna of an aphid non-transmissible plum pox virus (ppv-nat) isolate has been determined from five overlapping cdna clones. cdna prepared by primer extension was used to determine the 5' terminus. the assembled rna is 9741 nucleotides in length, excluding a 3' terminal poly(a) sequence. one large open reading frame starts at nucleotide positions 36 to 38 and is terminated with an uag codon at positions 9522 to 9524. the putative start codon is located at pos ...	1989	2732699
nucleotide sequence of the 3'-terminal region of potato virus yn rna.	the sequence of the 3'-terminal 1611 nucleotides of the genome of the tobacco veinal necrosis strain of potato virus y (pvyn) was determined. the sequence revealed an open reading frame of 1285 nucleotides, of which the start was not identified, and an untranslated region of 316 nucleotides upstream of a poly(a) tract. comparison of the open reading frame with the amino-terminal sequence of the viral coat protein enabled mapping of the start of the coat protein at amino acid -267, and indicated ...	1989	2732687
diet and nasopharyngeal carcinoma: a case-control study in guangzhou, china.	we conducted interviews on 306 histologically confirmed incident cases of nasopharyngeal carcinoma (npc) occurring in residents of guangzhou city, china, who were under the age of 50 years, and an equal number of age-, sex-, and neighborhood-matched controls. we also interviewed 110 mothers of patients under age 45 years and 139 mothers of controls who were matched to patients under age 45 years, to obtain information on childhood exposures of study subjects. exposure to salted fish, whether in ...	1989	2732001
proteolytic activity of the plum pox potyvirus nia-protein on excess of natural and artificial substrates in escherichia coli.	the plum pox potyvirus (ppv) nia protease expressed from a medium copy number plasmid was able to process an excess of substrate expressed from a high copy number plasmid, in a binary escherichia coli expression system. the delta b7 nia protease mutant only partially processed the nib-cp junction but its efficiency was independent of the amount of substrate. the delta b7 mutant essentially did not recognize an artificial cleavage site which was quite efficiently recognized by the wild-type prote ...	1989	2684687
proteolytic activity of the plum pox potyvirus nia-like protein in escherichia coli.	the nucleotide sequence of the small nuclear inclusion protein (nia)-like cistron of plum pox potyvirus (ppv) has been determined. viral proteolytic activity was expressed in escherichia coli cells harboring plasmids with a ppv cdna insert approximately 7000 nt long. free ppv capsid protein was detected in these cells, but it was not produced when a mutation was introduced in the ppv cdna insert which induced a gln to pro substitution at the large nuclear inclusion protein (nib)-capsid protein j ...	1989	2658302
artificial cleavage site recognized by plum pox potyvirus protease in escherichia coli.	a synthetic plum pox virus (ppv) nib-cp cleavage site was recognized by a ppv protease in an in vivo escherichia coli expression system. the presence of the natural nib-cp cleavage site did not affect processing at the artificial one. however, although both the proteases and the cleavage sites of ppv and tobacco etch virus show high sequence homology, a similar cassette from the tobacco etch virus nib-cp junction was not efficiently recognized by the ppv protease.	1989	2657098
expression of the plum pox virus coat protein region in escherichia coli.	a cdna complementary to the 3' end of plum pox virus (ppv) rna was sequenced. the sequence was investigated for the presumable coat protein cistron by computer-aided translation. a fragment containing the stop codon of the polyprotein gene and a putative virus-specific protease cleavage site was subcloned into an e. coli expression vector. it is shown by immunological analysis that the coat protein cistron is located within the subcloned region.	1989	2655276
[occurrence of nitrates and nitrites in certain frozen fruits, jams, stewed fruit and fruit-vegetable juices for children and in certain types of bee honey].	nitrates and nitrites were evaluated spectrophotometrically by the method of griess reaction, with previous reduction of nitrates to nitrites in a column filled with cadmium dust. the content of nitrates in frozen fruit (strawberries, black and red currant and plums) ranged from 2.50 to 57.38 mg kno3/kg, with the highest content in garden strawberries. in cherry, strawberry, black and red currant jams the concentrations were from 6.30 to 97.38 mg kno3/kg, the highest content was in cherry jam. i ...	1989	2637478
[atypical course of histiocytosis x].	a 48-year-old female patient was admitted because of increasing in size xanthomas of the eyelids lasting since 9 years (despite surgical treatment) and narrowing considerably the palpebral fissures. five years earlier the diagnosis of type ii b hyperlipoproteinaemia was made. presently, besides palpebral lesions, a painless plum-sized tumour was found in the right frontoparietal area and two hard tumours of similar size were situated bilaterally in the submandibular area. in the biopsy specimens ...	1989	2623863
the complete nucleotide sequence of plum pox virus rna (strain d).		1989	2602121
early screening for anti-plum pox virus monoclonal antibodies with different epitope specificities by means of gold-labelled immunosorbent electron microscopy.	the technique of gold-labelled immunosorbent electron microscopy for the initial screening of monoclonal antibodies 10 days after cell fusion from 96-well culture plates is described. the technique is used to identify clones that secrete antibodies binding on the surface of the virion or to viral subunits, and compared to elisa and western blotting. high sensitivity was demonstrated.	1988	2464610
infectious in vitro transcripts from a plum pox potyvirus cdna clone.	a full-length cdna clone of the 9786 nt plum pox virus (ppv) rna genome has been cloned downstream from a phage t7 rna polymerase promoter. the rnas synthesized by in vitro run-off transcription in the presence of the 5' cap analog m7gpppg were infectious in nicotiana clevelandii plants. no infectivity was detected when the transcriptions were carried out in the absence of the cap analog. inoculations of the local lesion host chenopodium foetidum indicated that the infectivity of the synthetic t ...	1990	2371774
mutational analysis of plum pox potyvirus polyprotein processing by the nia protease in escherichia coli.	a binary escherichia coli expression system has been used to study the pathway for proteolytic processing of the plum pox potyvirus (ppv) polyprotein. trans cleavage at the carboxyl end of the cylindrical inclusion protein occurred, although with lower efficiency than that at the large nuclear inclusion protein-capsid protein junction. no trans cleavage at the carboxyl end of the small nuclear inclusion protein (nia) was detected. the proteolytic activities at different cleavage sites of several ...	1990	2273380
[central neurogenic hyperventilation in an awake patient with a primary cerebral lymphoma].	we described clinical and neuropathological findings of a case of primary cerebral lymphoma with central neurogenic hyperventilation (cnh). a 54-year-old awake woman with a primary cerebral lymphoma presented hyperventilation for two weeks. arterial blood gas showed severe respiratory alkalosis; ph 7.603, paco2, 10.5 mmhg, pao2 129.8 mmhg, hco2 10.4 mmol/l, be -8.0, o2sat 98.9%. rebreathing from a paper bag, and intravenous administration of diazepam and sodium bicarbonate failed to alter the re ...	1990	2265512
rna helicase: a novel activity associated with a protein encoded by a positive strand rna virus.	most positive strand rna viruses infecting plants and animals encode proteins containing the so-called nucleotide binding motif (ntbm) (1) in their amino acid sequences (2). as suggested from the high level of sequence similarity of these viral proteins with the recently described superfamilies of helicase-like proteins (3-5), the ntbm-containing cylindrical inclusion (ci) protein from plum pox virus (ppv), which belongs to the potyvirus group of positive strand rna viruses, is shown to be able ...	1990	2263459
detection of the trans activity of the plum pox virus nia-like protease in infected plants.	the nia-like protein of plum pox virus is a protease with high sequence specificity that is autocatalytically released from the viral polyprotein. in order to determine whether the protease is active in trans we constructed a fusion protein consisting of the c-terminal region of the plum pox virus polyprotein and the staphylococcal protein a. the authentic protease recognition sequence asn-val-val-val-his-gln-ala occurs in the centre of this protein fusion. this protein was cleaved specifically ...	1990	2197372
dot hybridization detection of plum pox virus using 32p-labeled rna probes representing non-structural viral protein genes.	a cdna library covering the complete genome of plum pox virus strain d (ppv d) has been obtained, and an endonuclease restriction map derived from it. this map was superposed on the ppv genomic organisation map, established for a nonaphid transmissible strain of ppv (maiss et al., 1989). this allowed us to select seven probes, corresponding to different regions on the ppv genome. these probes were tested in a dot-blot hybridization assay for the detection of ppv. probes of various lengths (0.25 ...	1990	2148174
determination of polyprotein processing sites by amino terminal sequencing of nonstructural proteins encoded by plum pox potyvirus.	nonstructural proteins of plum pox potyvirus were partially purified following a procedure described for the isolation of tobacco etch virus nuclear inclusion proteins. plum pox virus proteins with electrophoretic mobilities corresponding to 49, 59 and 68 kda reacted with antibodies against the 49 kda and 54 kda components of the nuclear inclusions and the 70 kda component of the cylindrical inclusions of tobacco etch virus, respectively. further purification by size exclusion high performance l ...	1990	2138835
[40 cases of dengue (serotype 3) occurring in a military camp during an epidemic in new caledonia (1989). the value of vector control].	an epidemic of dengue occurred at the beginning of 1989 in new caledonia. about 18 p.c. of the population was stricken (25,000 to 30,000 estimated clinical cases). the military camp of plum was stricken too, but a prompt vector control was established. 8.6 p.c. of the strength was affected by classical dengue without severe hemorrhagic manifestations. such prevalence is lower than the one rated in the civil population, demonstrating the major importance of vector control to limit spreading of su ...	1990	2077321
plum pox potyvirus rna replication in a crude membrane fraction from infected nicotiana clevelandii leaves.	in vitro synthesis of plum pox potyvirus (ppv)-specific nucleic acid has been measured in a crude fraction prepared from leaves of ppv-infected nicotiana clevelandii plants. using alkali and dnase treatments, the synthesized nucleic acid was shown to be rna. the electrophoretic mobility and the differing sensitivity to rnase at high and low salt concentrations allowed the identification of in vitro products probably corresponding to replicative form and replicative intermediate rna, as well as t ...	1991	2016593
proteolytic activity of plum pox virus-tobacco etch virus chimeric nia proteases.	plasmids encoding chimeric nia-type proteases made of sequences from the potyviruses plum pox virus (ppv) and tobacco etch virus (tev) have been constructed. their proteolytic activity on the large nuclear inclusion protein (nib)-capsid protein (cp) junction of each virus was assayed in escherichia coli cells. the amino half of the protease seemed to be involved neither in the enzymatic catalysis nor in substrate recognition. in spite of the large homology among the ppv and tev nia-type protease ...	1991	2015911
identification of the initiation codon of plum pox potyvirus genomic rna.	the expression of plum pox potyvirus (ppv) genomic rna takes place through translation of its unique long and functional open reading frame (orf) into a large polyprotein that undergoes extensive proteolytic processing. in this paper we show that the aug recognized as the initiation codon of the ppv orf by in vitro translation systems is the one found at nucleotide position 147, in spite of the presence at position 36 of an in-phase aug that marks the start of the orf. deletion of a substantial ...	1991	1962436
rat preference for food-related odors.	preferences for food-related odors and the effects of fasting on those preferences were investigated during rat bar pressing for brief odor presentation. a rat was housed in an equilateral octagonal cage and had free access to food and water, except during fasting. among 8 food-related odor substances (black pepper, cheese, coffee, milk, nut, peppermint, plum and orange), black pepper, milk and coffee were most preferred, and cheese was least preferred, but even the bar pressing rate for cheese ...	1991	1959035
plum pit ileus: a case report.		1991	1944114
the complete nucleotide sequence of pea seed-borne mosaic virus rna.	the complete nucleotide sequence of the rna genome of pea seed-borne mosaic virus (psbmv) was determined from cloned cdna and by direct sequencing of viral rna. the psbmv genomic sequence was determined to be 9924 nucleotides in length excluding the poly(a) tract. the rna contained an open reading frame (orf) of 9618 nucleotides with the potential to encode a polyprotein with a calculated mr of 364000 (364k). the orf was flanked by a 5' untranslated leader sequence of 143 nucleotides and a 3' un ...	1991	1940858
a pcr membrane spot assay for the detection of plum pox virus rna in bark of infected trees.	a procedure for sensitive detection of plum pox virus rna in infected bark of trees is described. the method is based on the extraction of bark material with buffer containing proteinase k followed by partial purification of rna using quiagen anion exchange resin. the rna is then reverse transcribed, the single stranded cdna is amplified by the polymerase chain reaction using biotinylated deoxynucleotides as label. the amplified cdna can subsequently be detected by spotting the reaction mixture ...	1991	1864904
nucleotide sequence of the 3'-terminal region of the rna of the el amar strain of plum pox potyvirus.	the nucleotide sequence of the 3'-terminal 4773 nucleotides of the rna of a widely divergent, aphid-transmissible strain of plum pox potyvirus isolated from egypt (ppv-el amar) was determined. the sequenced region covers the carboxy terminus of the cylindrical inclusion (ci) gene, and the putative 6k protein, the nia protease, the nib rna polymerase and the coat protein genes, linked together as one large open reading frame (orf) in a fashion similar to the canonical genomic organization of othe ...	1991	1856701
novel catalytic activity associated with positive-strand rna virus infection: nucleic acid-stimulated atpase activity of the plum pox potyvirus helicaselike protein.	the cylindrical inclusion protein of potyviruses contains the so-called nucleoside triphosphate binding motif, an amino acid sequence motif present in proteins encoded by most positive-strand rna viruses, some double-strand rna viruses, apparently all groups of double-strand dna viruses, and also several single-strand dna viruses. further sequence analysis has allowed to include the cylindrical inclusion protein of potyviruses as a member of a superfamily of helicaselike proteins. in this paper ...	1991	1845877
a polymerase chain reaction assay adapted to plum pox potyvirus detection.	a sensitive, polyvalent assay based on the polymerase chain reaction (pcr) was developed for plum pox potyvirus (ppv) detection. this technique was adapted for a single tube, the chemical denaturation and reverse transcription of the viral rna followed by the pcr reaction yielding a 243-base-pair product. as few as 10 fg of purified viral rna, corresponding to approximately 2000 viral particles, were detected in plant extracts. all ppv isolates tested were amplified, and the amplified fragments ...	1991	1783677
shoulder dystocia: when to use suprapubic or fundal pressure.	"the delivery of the head with or without forceps may have been quite easy, but more commonly there has been a little difficulty in completing the extension of the head. the hairy scalp slides out with reluctance. when the forehead has appeared it is necessary to press back the perineum to deliver the face ... time passes. the child's head becomes suffused. it endeavors unsuccessfully to breathe. abdominal efforts by the mother or by her attendants produce no advance; gentle head traction is equ ...	1992	1738307
use of monoclonal antibodies for the identification of different antigenic domains in apple chlorotic leaf spot virus.	thirteen monoclonal antibodies (mabs) specific for apple chlorotic leaf spot virus (aclsv), produced by the somatic cell hybridization technique, were used to investigate the antigenic structure of the virus. epitope specificity studies showed that these mabs defined in aclsv particles seven independent antigenic domains, representing at least eight distinct epitopes. one of them was present only in virions and not in dissociated subunits. it appeared that the interaction between a mab and the v ...	1990	1700692
phylogenetic relationships in the stone fruit group of prunus as revealed by restriction fragment analysis of chloroplast dna.	in order to clarify the genetic relationships among stone fruits, a restriction fragment analysis of chloroplast dnas (cpdnas) was applied to cultivated prunus species, whose genetic diagnoses are difficult because of their heterogeneity and long life span. chloroplast dnas (cpdnas) were extracted from leaves of nine stone fruit accessions covering six species of prunus. a restriction fragment analysis was conducted by gel electrophoresis after digestion with these endonucleases. the genome size ...	1991	1676591
catalytic antisense rnas produced by incorporating ribozyme cassettes into cdna.	a simple strategy is described for the generation of catalytic hammerhead-type ribozymes (rz) that can be used as highly specific endoribonucleases to cleave a particular target rna. the technique requires that a cloned cdna fragment is available which encodes at least a part of the target rna. about 25 different restriction recognition sequences can be utilized to incorporate specifically designed dna cassettes into the cdna. besides some nucleotides which are specific for a certain restriction ...	1991	1660835
[struma carcinoid tumor within a dermoid cyst as an incidental finding in cesarean section].	a 25-year-old primigravida with breech presentation was admitted near term. during caesarean section, a dermoid cyst of the right ovary was detected. the dermoid cyst was enucleated, preserving a plum-sized ovary. histology revealed a strumal carcinoid within the excised dermoid cyst. from the histological point of view it was classified as a tumour of low malignancy. the clinical study provided no evidence of any metastases. on the 14th postoperative day the patient could be discharged from hos ...	1992	1607120
nucleotide sequence of the 3'-terminal region of potato virus a rna.	the sequence of the 3'-terminal region of the genome of the potato virus a (pva) was obtained from two independent cdna clones. this sequence is 1383 nucleotides long and contains an open reading frame of 1178 nucleotides, ending with the translation termination codon taa and followed by untranslated region of 205 nucleotides. since the n-terminal amino acid of the coat protein of pva was blocked, the position of the putative coat protein cleavage site has been deduced by searching for consensus ...	1992	1604933
proteolytic processing of the plum pox potyvirus polyprotein by the nia protease at a novel cleavage site.	the expression of potyvirus genomic rna takes place through translation of its unique long and functional open reading frame into a large polyprotein that undergoes extensive proteolytic processing. most of the cleavages are performed by the virus-encoded nia protease, which cuts the polyprotein at defined sites that are characterized by conserved heptapeptide sequences. we have demonstrated in vitro cleavage activity by the plum pox potyvirus (ppv) nia protease at a novel site, previously ident ...	1992	1585641