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rnai-mediated ultra-low gossypol cottonseed trait: performance of transgenic lines under field conditions.cottonseed remains a low-value by-product of lint production mainly due to the presence of toxic gossypol that makes it unfit for monogastrics. ultra-low gossypol cottonseed (ulgcs) lines were developed using rnai knockdown of δ-cadinene synthase gene(s) in gossypium hirsutum. the purpose of the current study was to assess the stability and specificity of the ulgcs trait and evaluate the agronomic performance of the transgenic lines. trials conducted over a period of 3 years show that the ulgcs ...201323078138
mapping quantitative trait loci for lint yield and fiber quality across environments in a gossypium hirsutum × gossypium barbadense backcross inbred line population.identification of stable quantitative trait loci (qtls) across different environments and mapping populations is a prerequisite for marker-assisted selection (mas) for cotton yield and fiber quality. to construct a genetic linkage map and to identify qtls for fiber quality and yield traits, a backcross inbred line (bil) population of 146 lines was developed from a cross between upland cotton (gossypium hirsutum) and egyptian cotton (gossypium barbadense) through two generations of backcrossing u ...201323064252
extraction and characterization of nanocellulose structures from raw cotton linter.this study aimed to characterize nanocellulose extracted from cotton (gossypium hirsutum) linters. the nanocellulose was subjected to electronic microscopy, thermal analysis, x-ray diffractometry, light scattering, and contact angle. the properties of the nanocellulose are considerably different from the linter. the acidic hydrolyses applied to extract the nanocrystals increased the crystallinity index and the hydrophilicity and decreased the thermal stability. on average, the nanocrystals were ...201323044127
molecular typing of mealybug phenacoccus solenopsis populations from different hosts and locations in punjab, india.true identity and existence of genetic variability in mealy bug from different regions holds immense significance for adopting appropriate control measures along with predicting the development of any biotypes. mealy bug, phenacoccus solenopsis adults were collected from four host plants i.e., gossypium hirsutum--cotton (c), abelmoschus esculentus--okra (o), pennisetum glaucum--napier bajra (b) and a weed--parthenium hysterophorus (p) in five cotton growing districts i.e., abohar, bathinda, mans ...201223029900
combining ability analysis for within-boll yield components in upland cotton (gossypium hirsutum l.).cotton is an important cash crop worldwide, accounting for a large percentage of world agricultural exports; however, yield per acre is still poor in many countries, including pakistan. diallel mating system was used to identify parents for improving within-boll yield and fiber quality parameters. combining ability analysis was employed to obtain suitable parents for this purpose. the parental genotypes cp-15/2, niab krishma, cim-482, ms-39, and s-12 were crossed in complete diallel mating under ...201223007974
global alteration of micrornas and transposon-derived small rnas in cotton (gossypium hirsutum) during cotton leafroll dwarf polerovirus (clrdv) infection.small rnas (srnas) are a class of non-coding rnas ranging from 20- to 40-nucleotides (nts) that are present in most eukaryotic organisms. in plants, srnas are involved in the regulation of development, the maintenance of genome stability and the antiviral response. viruses, however, can interfere with and exploit the silencing-based regulatory networks, causing the deregulation of srnas, including small interfering rnas (sirnas) and micrornas (mirnas). to understand the impact of viral infection ...201222987114
efficient decolorization and deproteinization using uniform polymer microspheres in the succinic acid biorefinery from bio-waste cotton (gossypium hirsutum l.) stalks.bio-waste cotton (gossypium hirsutum l.) stalks were converted into succinic acid by simultaneous saccharification and fermentation (ssf) using actinobacillus succinogenes 130z. after 54 h ssf at 40 °c and ph 7.0, the production of succinic acid was 63 g/l, with 1.17 g/l/h productivity and 64% conversion yield. after ssf, a simple method for the decolorization and deproteinization of crude ssf broth was developed through adsorption tests of polystyrene (pst) microspheres. under optimized conditi ...201322985822
efficient delivery of small interfering rna to plant cells by a nanosecond pulsed laser-induced stress wave for posttranscriptional gene silencing.small interfering rna (sirna) induced posttranscriptional gene silencing (ptgs) has been an efficient method for genetic and molecular analysis of certain developmental and physiological processes and represented a potential strategy for both controlling virus replication and developing therapeutic products. however, there are limitations for the methods currently used to deliver sirna into cells. we report here, to our knowledge, the first efficient delivery of sirna to plant cells by a nanosec ...200622980207
mechanism of phytohormone involvement in feedback regulation of cotton leaf senescence induced by potassium deficiency.to elucidate the phytohormonal basis of the feedback regulation of leaf senescence induced by potassium (k) deficiency in cotton (gossypium hirsutum l.), two cultivars contrasting in sensitivity to k deficiency were self- and reciprocally grafted hypocotyl-to-hypocotyl, using standard grafting (one scion grafted onto one rootstock), y grafting (two scions grafted onto one rootstock), and inverted y grafting (one scion grafted onto two rootstocks) at the seedling stage. k deficiency (0.03mm for s ...201222962680
interspecific chromosomal effects on agronomic traits in gossypium hirsutum by ad analysis using intermated g. barbadense chromosome substitution lines.the untapped potential of the beneficial alleles from gossypium barbadense l. has not been well utilized in g. hirsutum l. (often referred to as upland cotton) breeding programs. this is primarily due to genomic incompatibility and technical challenges associated with conventional methods of interspecific introgression. in this study, we used a hypoaneuploid-based chromosome substitution line as a means for systematically introgressing g. barbadense doubled-haploid line '3-79' germplasm into a c ...201322945267
lack of fitness costs associated with acetamiprid resistance in bemisia tabaci (hemiptera: aleyrodidae).sweetpotato whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae), is a devastating pest that can cause severe damage to a range of crops by direct feeding and by plant virus transmission. because of indiscriminate use of insecticides, this whitefly has developed resistance to several insecticides, including neonicotinoids. our objectives were to determine fitness components affected by acetamiprid resistance in b. tabaci. assay results showed that selection with acetamiprid had removed ...201222928322
the draft genome of a diploid cotton gossypium raimondii.we have sequenced and assembled a draft genome of g. raimondii, whose progenitor is the putative contributor of the d subgenome to the economically important fiber-producing cotton species gossypium hirsutum and gossypium barbadense. over 73% of the assembled sequences were anchored on 13 g. raimondii chromosomes. the genome contains 40,976 protein-coding genes, with 92.2% of these further confirmed by transcriptome data. evidence of the hexaploidization event shared by the eudicots as well as o ...201222922876
targeted capture of homoeologous coding and noncoding sequence in polyploid cotton.targeted sequence capture is a promising technology in many areas in biology. these methods enable efficient and relatively inexpensive sequencing of hundreds to thousands of genes or genomic regions from many more individuals than is practical using whole-genome sequencing approaches. here, we demonstrate the feasibility of target enrichment using sequence capture in polyploid cotton. to capture and sequence both members of each gene pair (homeologs) of wild and domesticated gossypium hirsutum, ...201222908041
arsenophonus groel interacts with clcuv and is localized in midgut and salivary gland of whitefly b. tabaci.cotton leaf curl virus (clcuv) (gemininiviridae: begomovirus) is the causative agent of leaf curl disease in cotton plants (gossypium hirsutum). clcuv is exclusively transmitted by the whitefly species b. tabaci (gennadius) (hemiptera: alerodidae). b. tabaci contains several biotypes which harbor dissimilar bacterial endo-symbiotic community. it is reported that these bacterial endosymbionts produce a 63 kda chaperon groel protein which binds to geminivirus particles and protects them from rapid ...201222900008
molecular markers associated with the immature fiber (im) gene affecting the degree of fiber cell wall thickening in cotton (gossypium hirsutum l.).cotton fiber fineness and maturity measured indirectly as micronaire (mic) are important properties of determining fiber grades in the textile market. to understand the genetic control and molecular mechanisms of fiber fineness and maturity, we studied two near isogenic lines, gossypium hirsutum, texas marker-1 wild type (tm-1) and immature fiber (im) mutant showing a significant difference in mic values. the fibers from im mutant plants were finer and less mature with lower mic values than thos ...201322890806
ghwrky15, a member of the wrky transcription factor family identified from cotton (gossypium hirsutum l.), is involved in disease resistance and plant development.as a large family of regulatory proteins, wrky transcription factors play essential roles in the processes of adaptation to diverse environmental stresses and plant growth and development. although several studies have investigated the role of wrky transcription factors during these processes, the mechanisms underlying the function of wrky members need to be further explored, and research focusing on the wrky family in cotton crops is extremely limited.201222883108
new insights into roles of cell wall invertase in early seed development revealed by comprehensive spatial and temporal expression patterns of ghcwin1 in cotton.despite substantial evidence on the essential roles of cell wall invertase (cwin) in seed filling, it remains largely unknown how cwin exerts its regulation early in seed development, a critical stage that sets yield potential. to fill this knowledge gap, we systematically examined the spatial and temporal expression patterns of a major cwin gene, ghcwin1, in cotton (gossypium hirsutum) seeds from prefertilization to prestorage phase. ghcwin1 messenger rna was abundant at the innermost seed coat ...201222864582
a chimeric arabinogalactan protein promotes somatic embryogenesis in cotton cell culture.arabinogalactan proteins (agps) are a family of extracellular plant proteoglycans implicated in many aspects of plant growth and development, including in vitro somatic embryogenesis (se). we found that specific agps were produced by cotton (gossypium hirsutum) calli undergoing se and that when these agps were isolated and incorporated into tissue culture medium, cotton se was promoted. when the agps were partly or fully deglycosylated, se-promoting activity was not diminished. testing of agps s ...201222858635
identification and analyses of mirna genes in allotetraploid gossypium hirsutum fiber cells based on the sequenced diploid g. raimondii genome.the plant genome possesses a large number of micrornas (mirnas) mainly 21-24 nucleotides in length. they play a vital role in regulation of target gene expression at various stages throughout the whole plant life cycle. here we sequenced and analyzed ≈ 10 million non-coding rnas (ncrnas) derived from fiber tissue of the allotetraploid cotton (gossypium hirsutum) 7 days post-anthesis using ncrna-seq technology. in terms of distinct reads, 24 nt ncrna is by far the dominant species, followed by 21 ...201222835981
the removal of reactive black 5 from aqueous solutions by cotton seed shell.this study investigated the removal of reactive black 5 (rb5) textile dye from aqueous solutions using cotton (gossypium hirsutum) seed shell (css) as low cost adsorbents. the data were described according to the freundlich and langmuir isotherm models. of these, the langmuir model provided the best fit for the experimental data. the highest measured adsorption density was 12.19 mg/g at ph 2. an equilibrium adsorption rate of rb5 by css (q(e) = 11.879 mg/g) was observed at 30 minutes. in order t ...201222834220
effects of entomopathogenic nematodes on evolution of pink bollworm resistance to bacillus thuringiensis toxin cry1ac.the evolution of resistance by pests can reduce the efficacy of transgenic crops that produce insecticidal toxins from bacillus thuringiensis (bt). however, fitness costs may act to delay pest resistance to bt toxins. meta-analysis of results from four previous studies revealed that the entomopathogenic nematode steinernema riobrave (rhabditida: steinernematidae) imposed a 20% fitness cost for larvae of pink bollworm, pectinophora gossypiella (saunders) (lepidoptera: gelechiidae), that were homo ...201222812141
sampling strategies for square and boll-feeding plant bugs (hemiptera: miridae) occurring on cotton.sampling methods for square and boll-feeding plant bugs (hemiptera: miridae) occurring on cotton, gossypium hirsutum l., were compared with the intent to assess if one approach was viable for two species occurring from early-season squaring to late bloom in 25 fields located along the coastal cotton growing region of south texas. cotton fleaphopper, pseudatomoscelis seriatus (reuter), damages squares early-season and dominated collections using five sampling methods (approximately 99% of insects ...201222812128
characterization of rhizobacterial strain rs-2 with acc deaminase activity and its performance in promoting cotton growth under salinity stress.a plant growth-promoting rhizobacterial strain rs-2 with 1-aminocyclopropane-1-carboxylate (acc) deaminase activity was isolated from salinized soils using acc as the sole nitrogen source. based on its physiological and biochemical properties and 16s rdna sequence analysis, this strain was identified as raoultella planticola. the maximum value of nitrogen fixation, dissolved phosphorus and dissolved potassium of rs-2 were 148.8 μg/ml, 205.0 and 4.31 mg/l, respectively within 192 h liquid culture ...201222806112
within-plant distribution of cotton aphid (hemiptera: aphididae) in cotton cultivars with colored fibers.we describe the vertical and horizontal distribution of the cotton aphid aphis gossypii glover within a cotton plant in two cotton (gossypium hirsutum linnaeus) cultivars (brs safira and brs rubí) with colored fiber over the time. measurements of aphid population dynamics and distribution in the cotton plants were recorded in intervals of seven days. the number of apterous or alate aphids and their specific locations were recorded, using as a reference point the location of nodes on the mainstem ...201222782536
cell suspension culture-mediated incorporation of the rice bel gene into transgenic cotton.cotton plants engineered for resistance to the herbicides, glyphosate or glufosinate have made a considerable impact on the production of the crop worldwide. in this work, embryogenic cell cultures derived from gossypium hirsutum l. cv coker 312 hypocotyl callus were transformed via agrobacterium tumefaciens with the rice cytochrome p450 gene, cyp81a6 (bel). in rice, bel has been shown to confer resistance to both bentazon and sulfanylurea herbicides. transformed cells were selected on a liquid ...201222768325
inheritance of resistance to meloidoygne incognita in primitive cotton accessions from mexico.few sources of resistance to root-knot nematodes (meloidogyne incognita) in upland cotton (gossypium hirsutum) have been utilized to develop resistant cultivars, making this resistance vulnerable to virulence in the pathogen population. the objectives of this study were to determine the inheritance of resistance in five primitive accessions of g. hirsutum (tx1174, tx1440, tx2076, tx2079, and tx2107) and to determine allelic relations with the genes for resistance in the genotypes clevewilt-6 (cw ...201022736869
impact of rotylenchulus reniformis on cotton yield as affected by soil texture and irrigation.the effects of soil type, irrigation, and population density of rotylenchulus reniformis on cotton were evaluated in a two-year microplot experiment. six soil types, fuquay sand, norfolk sandy loam, portsmouth loamy sand, muck, cecil sandy loam, and cecil sandy clay, were arranged in randomized complete blocks with five replications. each block had numerous plots previously inoculated with r. reniformis and two or more noninoculated microplots per soil type, one half of which were irrigated in e ...201022736865
natural migration of rotylenchulus reniformis in a no-till cotton system.rotylenchulus reniformis is the most damaging nematode pathogen of cotton in alabama. it is easily introduced into cotton fields via contaminated equipment and, when present, is difficult and costly to control. a trial to monitor the natural migration of r. reniformis from an initial point of origin was established in 2007 and studied over two growing seasons in both irrigated and non-irrigated no-till cotton production systems. vermiform females, juveniles and males reached a horizontal distanc ...201022736863
molecular and morphological characterization and biological control capabilities of a pasteuria ssp. parasitizing rotylenchulus reniformis, the reniform nematode.rotylenchulus reniformis is one of 10 described species of reniform nematodes and is considered the most economically significant pest within the genus, parasitizing a variety of important agricultural crops. rotylenchulus reniformis collected from cotton fields in the southeastern us were observed to have the nematode parasitic bacterium pasteuria attached to their cuticles. challenge with a pasteuria-specific monoclonal antibody in live immuno-fluorescent assay (ifa) confirmed the discovery of ...201022736858
classification of rotylenchulus reniformis numbers in cotton using remotely sensed hyperspectral data on self-organizing maps.rotylenchulus reniformis is one of the major nematode pests capable of reducing cotton yields by more than 60%, causing estimated losses that may exceed millions of dollars u.s. therefore, early detection of nematode numbers is necessary to reduce these losses. this study investigates the feasibility of using remotely sensed hyperspectral data (reflectances) of cotton plants affected with different nematode population numbers with self-organizing maps (som) in correlating and classifying nematod ...201022736855
effect of mowing cotton stalks and preventing plant re-growth on post-harvest reproduction of meloidogyne incognita.the southern root-knot nematode (meloidogyne incognita) is a major parasite of cotton in the u.s., and management tactics for this nematode attempt to minimize population levels. we compared three post-harvest practices for their ability to reduce nematode population levels in the field, thereby reducing initial nematode population for the next year's crop. the three practices tested were: 1) chemical defoliation before harvest plus cutting cotton stalks after harvest, 2) chemical defoliation pl ...201022736845
population dynamics of meloidogyne arenaria and pasteuria penetrans in a long-term crop rotation study.the endospore-forming bacterium pasteuria penetrans is an obligate parasite of root-knot nematodes (meloidogyne spp.). the primary objective of this study was to determine the effect of crop sequence on abundance of p. penetrans. the experiment was conducted from 2000 to 2008 at a field site naturally infested with both the bacterium and its host meloidogyne arenaria and included the following crop sequences: continuous peanut (arachis hypogaea) (p-p-p) and peanut rotated with either 2 years of ...200922736828
cotton gene expression profiles in resistant gossypium hirsutum cv. zhongzhimian kv1 responding to verticillium dahliae strain v991 infection.verticillium wilt of cotton (gossypium hirsutum) is a widespread and destructive disease that is caused by the soil-borne fungus pathogen verticillium dahliae (v. dahliae). to study the molecular mechanism in wilt tolerance, suppression subtractive hybridization (ssh) and dot blot techniques were used to identify the specifically expressed genes in a superior wilt-resistant cotton cultivar (g. hirsutum cv. zhongzhimian kv1) after inoculation with pathogen. cdnas from the root tissues of zhongzhi ...201222733494
molecular diversity, genomic constitution, and qtl mapping of fiber quality by mapped ssrs in introgression lines derived from gossypium hirsutum × g. darwinii watt.because the genetic basis of current upland cotton cultivars is narrow, exploring new germplasm resources and discovering novel alleles relevant to important agronomic traits have become two of the most important themes in the field of cotton research. in this study, g. darwinii watt, a wild cotton species, was crossed with four upland cotton cultivars with desirable traits. a total of 105 introgression lines (ils) were successfully obtained. by using 310 mapped ssrs evenly distributed across th ...201222733444
[monitoring and simulation of soil electrical conductivity based on the hyperspectral parameters of cotton (gossypium hirsutum) functional leaves].taking the salt-tolerant cotton variety ccri-44 and salt-sensitive cotton variety sumian 12 as test materials, a two-year pot experiment was conducted at the pailou experimental station of nanjing agricultural university in 2008 and 2009 to study the relationships of soil electrical conductivity (ec) with the spectral reflectance and hyperspectral indices of cotton functional leaves at different growth stages under five simulated salinity levels (0, 0.35%, 0.60%, 0.85%, and 1.00%) of coastal sal ...201222720615
molecular characterization of an elicitor-responsive armadillo repeat gene (gharm) from cotton (gossypium hirsutum).only a few armadillo (arm) repeat proteins have been characterized in plants where they appear to have diverse functions, including the regulation of defence responses. in this study, the identification, cloning and characterization of a gene, encoding an arm repeat protein (gharm), is described. gharm exists as multiple copies in cotton, with an 1713 bp orf encoding 570 amino acids. the predicted protein contains three consecutive arm repeats within an armadillo-type fold, with no other disting ...201222714909
genome-wide identification of differentially expressed genes under water deficit stress in upland cotton (gossypium hirsutum l.).cotton is the world's primary fiber crop and is a major agricultural commodity in over 30 countries. like many other global commodities, sustainable cotton production is challenged by restricted natural resources. in response to the anticipated increase of agricultural water demand, a major research direction involves developing crops that use less water or that use water more efficiently. in this study, our objective was to identify differentially expressed genes in response to water deficit st ...201222703539
the multi-year effects of repeatedly growing cotton with moderate resistance to meloidogyne incognita.meloidogyne incognita causes more damage to cotton in the us than any other pathogen. the objective of this study was to document the cumulative effect of moderate resistance on m. incognita population density, root galling, and yield suppression in the southern united states on a moderately resistant cotton genotype grown continuously for three years. cotton genotypes were phytogen ph98-3196 (77% suppression of m. incognita), acala nemx (85% suppression of m. incognita), and delta and pine land ...200922661787
reproduction of meloidogyne marylandi and m. incognita on several poaceae.the susceptibility of 22 plant species to meloidogyne marylandi and m. incognita was examined in three greenhouse experiments. inoculum of m. marylandi was eggs from cultures maintained on zoysia matrella "cavalier" or cynodon dactylon x c. trasvaalensis "tifdwarf". inoculum of m. incognita was eggs from cultures maintained on solanum lycopersicum 'rutgers'. in each host test the inoculum density was 2,000 nematode eggs/pot. none of the three dicot species tested (gossypium hirsutum, arachis hyp ...200922661770
expression of atsap5 in cotton up-regulates putative stress-responsive genes and improves the tolerance to rapidly developing water deficit and moderate heat stress.the regulation of gene expression is a key factor in plant acclimation to stress, and it is thought that manipulation of the expression of critical stress-responsive genes should ultimately provide increased protection against abiotic stress. the aim of this study was to test the hypothesis that the ectopic expression of the atsap5 (at3g12630) gene in transgenic cotton (gossypium hirsutum, cv. coker 312) will improve tolerance to drought and heat stress by up-regulating the expression of endogen ...201222633820
geminivirus-mediated delivery of florigen promotes determinate growth in aerial organs and uncouples flowering from photoperiod in cotton.plant architecture and the timing and distribution of reproductive structures are fundamental agronomic traits shaped by patterns of determinate and indeterminate growth. florigen, encoded by flowering locus t (ft) in arabidopsis and single flower truss (sft) in tomato, acts as a general growth hormone, advancing determinate growth. domestication of upland cotton (gossypium hirsutum) converted it from a lanky photoperiodic perennial to a highly inbred, compact day-neutral plant that is managed a ...201222615805
overexpression of cotton (gossypium hirsutum) dirigent1 gene enhances lignification that blocks the spread of verticillium dahliae.dirigent super-family abounds throughout the plant kingdom, especially vascular plants. to elucidate the function of cotton (gossypium hirsutum) dir genes in lignification, two cdnas (designated ghdir1 and ghdir2) encoding putative dirigent proteins were isolated from cotton cdna libraries. real-time quantitative reverse transcription-polymerase chain reaction analysis revealed that ghdir1 transcript was preferentially accumulated in cotton hypocotyls, whereas ghdir2 was predominantly expressed ...201222595512
effects of corn processing method and dietary inclusion of wet distillers grains with solubles on energy metabolism, carbon-nitrogen balance, and methane emissions of cattle.the growing ethanol industry in the southern great plains has increased the use of wet distillers grains with solubles (wdgs) in beef cattle (bos taurus) finishing diets. few studies have used steam-flaked corn (zea mays l.; sfc)-based diets to evaluate the effects of wdgs in finishing cattle diets, and a reliable estimate of the net energy value of wdgs has yet to be determined. effects of corn processing method and wdgs on energy metabolism, c and n balance, and enteric methane (ch(4)) product ...201222585790
identification of rna editing sites in cotton (gossypium hirsutum) chloroplasts and editing events that affect secondary and three-dimensional protein structures.rna editing can alter individual nucleotides in primary transcripts, which can cause the amino acids encoded by edited rna to deviate from the ones predicted from the dna template. we investigated rna editing sites of protein-coding genes from the chloroplast genome of cotton. fifty-four editing sites were identified in 27 transcripts, which is the highest editing frequency found until now in angiosperms. all these editing sites were c-to-u conversion, biased toward ndh genes and u_a context. ex ...201222576925
cloning and expression analysis of novel aux/iaa family genes in gossypium hirsutum.members of the aux/iaa gene family encode proteins that mediate the responses of auxin-regulated gene expression and regulate various aspects of plant morphological development. here, we provide the first identification and characterization of nine cdnas encoding the complete open reading frame (orf) of the aux/iaa family in cotton. these were designated ghaux1 to ghaux9 (gossypiumhirsutum aux/iaa). the proteins encoded by these nine genes had either whole or partially conserved domains of the a ...201222575728
dominant gene cpls(r)1 corresponding to premature leaf senescence resistance in cotton (gossypium hirsutum l.).cotton (gossypium hirsutum l.) premature leaf senescence-resistant inbred xlz33 and senescence-susceptible inbred lines xlz13 were selected and crossed to produce f(1), f(1)-reciprocal, f(2) and bc(1) generations for evaluation of leaf senescence process and inheritance. the results showed that leaf senescence processes for xlz13 and xlz33 were obviously different and leaf senescence traits could be distinguished between the two parents at particular periods of cotton growth. inheritance anlysis ...201222574717
formation of tetrahydrocurcumin by reduction of curcumin with cultured plant cells of marchantia polymorpha.cultured plant cells of marchantia polymorpha, nicotiana tabacum, phytolacca americana, catharanthus roseus, and gossypium hirsutum were examined for their ability to reduce curcumin. only m. polymorpha cells converted curcumin into tetrahydrocurcumin in 90% yield in one day. time-course experiment revealed a two-step formation of tetrahydrocurcumin via dihydrocurcumin.201222574459
chilling stress--the key predisposing factor for causing alternaria alternata infection and leading to cotton (gossypium hirsutum l.) leaf senescence.leaf senescence plays a vital role in nutrient recycling and overall capacity to assimilate carbon dioxide. cotton premature leaf senescence, often accompanied with unexpected short-term low temperature, has been occurring with an increasing frequency in many cotton-growing areas and causes serious reduction in yield and quality of cotton. the key factors for causing and promoting cotton premature leaf senescence are still unclear. in this case, the relationship between the pre-chilling stress a ...201222558354
genome-wide profiling of mirnas and other small non-coding rnas in the verticillium dahliae-inoculated cotton roots.micrornas (mirnas) and small interfering rnas (sirnas) are short (19-25 nucleotides) non-coding rna molecules that have large-scale regulatory effects on development and stress responses in plants. verticillium wilt is a vascular disease in plants caused by the fungal pathogen verticillium dahliae. the objective of this study is to investigate the transcriptional profile of mirnas and other small non-coding rnas in verticillium-inoculated cotton roots. four small rna libraries were constructed f ...201222558219
a novel mesh processing based technique for 3d plant analysis.in recent years, imaging based, automated, non-invasive, and non-destructive high-throughput plant phenotyping platforms have become popular tools for plant biology, underpinning the field of plant phenomics. such platforms acquire and record large amounts of raw data that must be accurately and robustly calibrated, reconstructed, and analysed, requiring the development of sophisticated image understanding and quantification algorithms. the raw data can be processed in different ways, and the pa ...201222553969
the cotton centromere contains a ty3-gypsy-like ltr retroelement.the centromere is a repeat-rich structure essential for chromosome segregation; with the long-term aim of understanding centromere structure and function, we set out to identify cotton centromere sequences. to isolate centromere-associated sequences from cotton, (gossypium hirsutum) we surveyed tandem and dispersed repetitive dna in the genus. centromere-associated elements in other plants include tandem repeats and, in some cases, centromere-specific retroelements. examination of cotton genomic ...201222536361
host preference of cotton fleahopper, pseudatomoscelis seriatus (reuter) is not labile to geographic origin and prior experience.several phytophagous insects exhibit distinct preference for their host plants. in widely distributed generalist insects, host preference can be influenced by geographic variation in host plant distribution and abundance as well as by prior experience. we have studied host preference of the cotton fleahopper, pseudatomoscelis seriatus (reuter), a pest of cotton in texas and other neighboring states, by measuring olfactory orientation to horsemint (monarda punctata l.) and cotton (gossypium hirsu ...201222525067
aphid antixenosis in cotton is activated by the natural plant defence elicitor cis-jasmone.upon insect herbivory, plants can release blends of volatile organic compounds (vocs) that modify herbivore and natural enemy behaviour. we have shown recently that cotton, gossypium hirsutum, emits a blend of defence vocs that repels the cotton aphid, aphis gossypii, upon herbivory by this notorious crop pest, including (z)-3-hexenyl acetate, (e)-4,8-dimethyl-1,3,7-nonatriene (dmnt), methyl salicylate and (e,e)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (tmtt). in this study, we investigated cha ...201222516741
epidermal cell differentiation in cotton mediated by the homeodomain leucine zipper gene, ghhd-1.gossypium hirsutum l. (cotton) fibres are specialized trichomes a few centimetres in length that grow from the seed coat. few genes directly involved in the differentiation of these epidermal cells have been identified. these include ghmyb25-like and ghmyb25, two related myb transcription factors that regulate fibre cell initiation and expansion. we have also identified a putative homeodomain leucine zipper (hd-zip) transcription factor, ghhd-1, expressed in trichomes and early fibres that might ...201222443311
cotton ghmkk5 affects disease resistance, induces hr-like cell death, and reduces the tolerance to salt and drought stress in transgenic nicotiana benthamiana.mitogen-activated protein kinase (mapk) cascades are involved in various processes from plant growth and development to biotic and abiotic stress responses. mapk kinases (mapkks), which link mapks and mapkk kinases (mapkkks), play crucial roles in mapk cascades to mediate a variety of stress responses in plants. however, few mapkks have been functionally characterized in cotton (gossypium hirsutum). in this study, a novel gene, ghmkk5, from cotton belonging to the group c mapkks was isolated and ...201222442420
genome physical mapping of polyploids: a bibac physical map of cultivated tetraploid cotton, gossypium hirsutum l.polyploids account for approximately 70% of flowering plants, including many field, horticulture and forage crops. cottons are a world-leading fiber and important oilseed crop, and a model species for study of plant polyploidization, cellulose biosynthesis and cell wall biogenesis. this study has addressed the concerns of physical mapping of polyploids with bacs and/or bibacs by constructing a physical map of the tetraploid cotton, gossypium hirsutum l. the physical map consists of 3,450 bibac c ...201222438974
overexpression of the atlos5 gene increased abscisic acid level and drought tolerance in transgenic cotton.drought is the major environmental stress that limits cotton (gossypium hirsutum l.) production worldwide. los5/aba3 (los5) encodes a molybdenum co-factor and is essential for activating aldehyde oxidase, which is involved in abscisic acid (aba) biosynthesis. in this study, a los5 cdna of arabidopsis thaliana was overexpressed in cotton cultivar zhongmiansuo35 (z35) by agrobacterium tumefaciens-mediated transformation. the transformation and overexpression of atlos5 were assessed by pcr and rt-p ...201222412184
genotypic variations in photosynthetic and physiological adjustment to potassium deficiency in cotton (gossypium hirsutum).a hydroponic culture experiment was conducted to determine genotypic variation in photosynthetic rate and the associated physiological changes in response to potassium (k) deficiency in cotton (gossypium hirsutum l.) seedlings with contrasting two cotton cultivars in k efficiency. the k-efficient liaomian18 produced 66.7% more biomass than the k-inefficient nucotn99(b) under k deficiency, despite their similar biomass under k sufficiency. compared with nucotn99(b), liaomian18 showed 19.4% higher ...201222387141
a high-density simple sequence repeat and single nucleotide polymorphism genetic map of the tetraploid cotton genome.genetic linkage maps play fundamental roles in understanding genome structure, explaining genome formation events during evolution, and discovering the genetic bases of important traits. a high-density cotton (gossypium spp.) genetic map was developed using representative sets of simple sequence repeat (ssr) and the first public set of single nucleotide polymorphism (snp) markers to genotype 186 recombinant inbred lines (rils) derived from an interspecific cross between gossypium hirsutum l. (tm ...201222384381
molecular evolution and phylogenetic analysis of genes related to cotton fibers development from wild and domesticated cotton species in gossypium.the domestication of both diploid and tetraploid cotton species was carried out for fiber utilization. to understand the origin and domestication of fibers, 18 genes related to fiber development were individually cloned and sequenced from 22 different cotton species. their structures, phylogenetic relationship and molecular evolution were further studied. in the orthologous and homeologous loci of the 18 genes, the sequence and structure of 72.22% were conserved and 27.78% were diverse. tree top ...201222381639
hemigossypol, a constituent in developing glanded cottonseed (gossypium hirsutum).gossypol is a dimeric sesquiterpenoid first identified in cottonseed, but found in various tissues in the cotton plant including the seed. from its first discovery, it was assumed that hemigossypol was the biosynthetic precursor of gossypol. previous studies established that peroxidase (either from horseradish or from cottonseed) converts hemigossypol to gossypol. however, hemigossypol has never been identified in healthy cottonseed. in a temporal study using hplc and lc-ms, hemigossypol was ide ...201222369216
microsatellite loci for gossypium davidsonii (malvaceae) and other d-genome, sonoran desert endemic cotton species.microsatellite primers previously developed for domesticated cotton (gossypium hirsutum; tetraploid) were screened for their utility in investigating genetic structure and gene flow within g. davidsonii and five other wild, mexican, d-genome cotton species (all diploid).201222358044
spatial mapping of lipids at cellular resolution in embryos of cotton.advances in mass spectrometry (ms) have made comprehensive lipidomics analysis of complex tissues relatively commonplace. these compositional analyses, although able to resolve hundreds of molecular species of lipids in single extracts, lose the original cellular context from which these lipids are derived. recently, high-resolution ms of individual lipid droplets from seed tissues indicated organelle-to-organelle variation in lipid composition, suggesting that heterogeneity of lipid distributio ...201222337917
floral to green: mating switches moth olfactory coding and preference.mating induces profound physiological changes in a wide range of insects, leading to behavioural adjustments to match the internal state of the animal. here, we show for the first time, to our knowledge, that a noctuid moth switches its olfactory response from food to egg-laying cues following mating. unmated females of the cotton leafworm (spodoptera littoralis) are strongly attracted to lilac flowers (syringa vulgaris). after mating, attraction to floral odour is abolished and the females fly ...201222319127
phytosanitary irradiation of liriomyza trifolii (diptera: agromyzidae).agromyzid leafminers are economic and quarantine pests of a variety of vegetables, flowers, and ornamental foliage. methyl bromide fumigation is often used as a phytosanitary treatment when quarantined agromyzids are found in shipped commodities; alternative treatments are sought. ionizing radiation is a viable alternative that is increasing in use worldwide. a dose of 400 gy is accepted by usda-aphis for all insects (except lepidoptera pupae and adults) on all commodities. efforts to lower this ...201122299344
inheritance of long staple fiber quality traits of gossypium barbadense in g. hirsutum background using csils.gossypium hirsutum is a high yield cotton species that exhibits only moderate performance in fiber qualities. a promising but challenging approach to improving its phenotypes is interspecific introgression, the transfer of valuable traits or genes from the germplasm of another species such as g. barbadense, an important cultivated extra long staple cotton species. one set of chromosome segment introgression lines (csils) was developed, where tm-1, the genetic standard in g. hirsutum, was used as ...201222297564
development and mapping of snp assays in allotetraploid cotton.a narrow germplasm base and a complex allotetraploid genome have made the discovery of single nucleotide polymorphism (snp) markers difficult in cotton (gossypium hirsutum). to generate sequence for snp discovery, we conducted a genome reduction experiment (ecori, bafi double digest, followed by adapter ligation, biotin-streptavidin purification, and agarose gel separation) on two accessions of g. hirsutum and two accessions of g. barbadense. from the genome reduction experiment, a total of 2.04 ...201222252442
influence of corn on stink bugs (heteroptera: pentatomidae) in subsequent crops.in southeastern united states farmscapes, corn, zea mays l., is often closely associated with peanut, (arachis hypogaea l.), cotton, (gossypium hirsutum l.), or both. the objective of this 3-yr on-farm study was to examine the influence of corn on stink bugs (heteroptera: pentatomidae), nezara viridula (l.), and euschistus servus (say), in subsequent crops in these farmscapes. adults of both stink bug species entered corn first, and seasonal occurrence of stink bug eggs, nymphs, and adults indic ...201122251727
protein expression changes during cotton fiber elongation in response to low temperature stress.low temperature stress is one of the major abiotic stresses limiting the formation of cotton (gossypium hirsutum l.) fiber qualities, especially fiber length. to investigate the molecular adaptation mechanisms of cotton fiber elongation to low temperature stress, two cotton cultivars, kemian 1 (low temperature-tolerant) and sumian 15 (low temperature-sensitive), were planted in the field at two sowing dates (25 april and 10 june). the two sowing dates resulted in different growing conditions and ...201222244703
ghagp31, a cotton non-classical arabinogalactan protein, is involved in response to cold stress during early seedling development.arabinogalactan proteins (agps), a superfamily of highly glycosylated hydroxyproline-rich glycoproteins, are widely implicated in plant growth and development. a gene (including its cdna), designated ghagp31, encoding a non-classical agp protein was isolated from cotton (gossypium hirsutum). the deduced ghagp31 protein contains the conserved features of non-classical agps: a putative signal peptide, n-terminal histidine-rich stretch, middle repetitive proline-rich domain and a cysteine-containin ...201222222112
[gene mapping of an ultra-dwarf mutant in upland cotton].a ultra-dwarf mutant was newly found in upland cotton (gossypium hirsutum l.). this mutant was controlled by single recessive gene du. seventy pairs of polymorphic primers were selected from 1350 primers, which covered all the identified chromosomes by screening in parents and near-isogenic lines. gene du was mapped using an f2 population derived from intraspecific crosses between "ultra-dwarf 1" and "xinluzao 16". thirty-six primers were distributed in eight linkage groups, and du was linked to ...201122207386
cloning and characterization of homeologous cellulose synthase catalytic subunit 2 genes from allotetraploid cotton (gossypium hirsutum l.).cellulose synthase catalytic subunits (cesas) are the catalytic sites within a multisubunit complex for cellulose biosynthesis in plants. cesas have been extensively studied in diploid plants, but are not well characterized in polyploid plants. gossypium hirsutum is an allotetraploid cotton species producing over 90% of the world's cotton fibers. although g. hirsutum cesas (ghcesas) are responsible for cellulose production in cotton fiber, very limited numbers of ghcesa genes have been identifie ...201222200568
effect of reproductive mode on host plant utilization of melon aphid (hemiptera: aphididae).variation in the reproductive mode of melon aphid aphis gossypii glover occurred on the large geographic scale, but the performance of different reproductive modes to use host plant is poorly understood. life tables of melon aphid population that undergo the anholocyclic, androcyclic, and intermediate reproductive mode were conducted on different host plants. the results showed that the anholocyclic and androcyclic strains could become adults and produce offspring on cotton gossypium hirsutum l. ...201022182553
generation of ests for flowering gene discovery and ssr marker development in upland cotton.upland cotton, gossypium hirsutum l., is one of the world's most important economic crops. in the absence of the entire genomic sequence, a large number of expressed sequence tag (est) resources of upland cotton have been generated and used in several studies. however, information about the flower development of this species is rare.201122163052
difference in mirna expression profiles between two cotton cultivars with distinct salt sensitivity.micrornas (mirnas) are a class of endogenous, non-coding small rnas that play important roles in many developmental processes and stress responses in plants and animals. cotton (gossypium hirsutum l.) is considered a relatively salt-tolerant non-halophytic plant species. to study the role of mirnas in salt adaptation, a salt-tolerant cotton cultivar sn-011 and a salt-sensitive cultivar lm-6 were used to detect differentially expressed mirnas. using mirna microarray analysis and a computational a ...201222160515
cotton shoot plays a major role in mediating senescence induced by potassium deficiency.the objective of this study was to determine the roles of shoot and root in the regulation of premature leaf senescence induced by potassium (k) deficiency in cotton (gossypium hirsutum l.). two contrasting cultivars (ccri41, more sensitive to k deficiency; and scrc22, a less sensitive cultivar) were selected for self- and reciprocal-grafting, using standard grafting (one scion/one rootstock), y grafting (two scions/one rootstock) and inverted y grafting (one scion/two rootstocks) at the seedlin ...201222154601
remotely sensing arthropod and nutrient stressed plants: a case study with nitrogen and cotton aphid (hemiptera: aphididae).remote sensing can be used in combination with ground sampling to detect aphid- (aphis gossypii glover) infested cotton (gossypium hirsutum l.). changes in wavelengths in the near-infrared (nir) have proven useful for such detection, but these changes can be confused with other factors stressing plants, such as water deficiency and nutrient status. this study was designed to test the utility of this technology to distinguish between two factors stressing plants: nitrogen deficiency and aphids. f ...201022127176
oviposition and development of the tarnished plant bug (heteroptera: miridae) on field maize.reduced insecticide use in cotton, gossypium hirsutum l., as a consequence of the boll weevil eradication program and the broad adoption of bt cotton, have helped make the tarnished plant bug, lygus lineolaris (palisot de beauvois), a consistent pest of cotton each year in the mid-south. maize, zea mays l., has been implicated as having a role in the season-long dynamics of tarnished plant bug infestations in cotton. to date, no published information exists describing the quality of maize as a ...201022127158
[salt tolerance evaluation of cotton (gossypium hirsutum) at its germinating and seedling stages and selection of related indices].a sand culture experiment was conducted to study the salt tolerance of 13 cotton cultivars at their germinating and seedling stages under the stress of different concentration nacl, and a cluster analysis was made on the salt tolerance, according to the subjection values of salt toxicity coefficients of multi-indices and the sum subjection value. it was observed that the appropriate concentration of nacl for the evaluation of salt tolerance was 150 mmol x l(-1). the salt tolerance differed with ...201122097366
cdna-aflp-based genetical genomics in cotton fibers.genetical genomics, or genetic analysis applied to gene expression data, has not been widely used in plants. we used quantitative cdna-aflp to monitor the variation in the expression level of cotton fiber transcripts among a population of inter-specific gossypium hirsutum × g. barbadense recombinant inbred lines (rils). two key fiber developmental stages, elongation (10 days post anthesis, dpa), and secondary cell wall thickening (22 dpa), were studied. normalized intensity ratios of 3,263 and 1 ...201222080217
susceptibility of spodoptera frugiperda (lepidoptera: noctuidae) strains from central colombia to two insecticides, methomyl and lambda-cyhalothrin: a study of the genetic basis of resistance.spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae) is an important pest of several crops in the western hemisphere. this insect has genetically differentiated into two host-associated populations: the corn (zea mays l.) and the rice (oryza sativa l.) strains. the corn strain also is found in cotton (gossypium hirsutum l.) and sorghum and the rice strain in bermuda grass [cynodon dactylon (l.) pers.] and millet. in the united states and brazil, lines from corn, rice, bermuda grass, and ...201122066201
overexpression of ghsusa1 increases plant biomass and improves cotton fiber yield and quality.cotton (gossypium spp.) is an important economic crop and the largest source of textile fiber in the world. however, to date, only a few genes have been identified that exhibit critical roles in fiber development, and few has shown positive effects on fiber yield and quality in transgenic cotton. here, we report the characterization of a novel sucrose synthase (susa1) gene from a superior quality fiber germplasm line 7235 in gossypium hirsutum. by association analysis, ghsusa1 was highly correla ...201222044435
phylogeny and pathogenicity of fusarium oxysporum isolates from cottonseed imported from australia into california for dairy cattle feed.a unique biotype of the fusarium wilt pathogen, fusarium oxysporum schlecht. f.sp. vasinfectum (atk) sny. & hans., found in australia in 1993 is favored by neutral or alkaline heavy soils and does not require plant parasitic nematodes to cause disease. this makes it a threat to 4-6 million acres of usa upland cotton ( gossypium hirsutum l.) that is grown on heavy alkaline soil and currently is not affected by fusarium wilt. in 2001-2002, several shiploads of live cottonseed were imported into ca ...201122004096
Efficient free fatty acid production in Escherichia coli using plant acyl-ACP thioesterases.Microbial biosynthesis of fatty acid-like chemicals from renewable carbon sources has attracted significant attention in recent years. Free fatty acids can be used as precursors for the production of fuels or chemicals. Free fatty acids can be produced by introducing an acyl-acyl carrier protein thioesterase gene into Escherichia coli. The presence of the acyl-ACP thioesterase will break the fatty acid elongation cycle and release free fatty acid. Depending on their sequence similarity and subst ...201122001432
Overexpression of a cotton cyclophilin gene (GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pv. tabaci infection.The full-length cDNA of a cyclophilin-like gene was cloned from Gossypium hirsutum using rapid amplification of cDNA ends and was designated as GhCyp1, a member of the immunophilin protein family. GhCyp1 expression level was higher in roots and stems than in other tissues of cotton, as determined by real-time reverse transcription polymerase chain reaction (RT-PCR). To characterize the GhCyp1 gene, tobacco (Nicotiana tabacum) was transformed via Agrobacterium tumefaciens with a vector to express ...201122000049
genetic and epigenetic status of triple exotic consanguinity cotton introgression lines.introgression lines are some of the most important germplasm for breeding applications and other research conducted on cotton crops. the dna methylation level among 10 introgression lines of cotton (gossypium hirsutum) and three exotic parental species (g. arboreum, g. thurberi and g. barbadense) were assessed by methylation-sensitive amplified polymorphism (msap) technology. the methylation level in the introgression lines ranged from 33.3 to 51.5%. however, the lines pd0111 and pd0113 had the ...201121968805
a combined functional and structural genomics approach identified an est-ssr marker with complete linkage to the ligon lintless-2 genetic locus in cotton (gossypium hirsutum l.).cotton fiber length is an important quality attribute to the textile industry and longer fibers can be more efficiently spun into yarns to produce superior fabrics. there is typically a negative correlation between yield and fiber quality traits such as length. an understanding of the regulatory mechanisms controlling fiber length can potentially provide a valuable tool for cotton breeders to improve fiber length while maintaining high yields. the cotton (gossypium hirsutum l.) fiber mutation li ...201121902843
genomically biased accumulation of seed storage proteins in allopolyploid cotton.allopolyploidy is an important process during plant evolution that results in the reunion of two divergent genomes into a common nucleus. many of the immediate as well as longer-term genomic and epigenetic responses to polyploidy have become appreciated. to investigate the modifications of gene expression at the proteome level caused by allopolyploid formation, we conducted a comparative analysis of cotton seed proteomes from the allopolyploid gossypium hirsutum (ad genome) and its model a-genom ...201121900265
recent long-distance transgene flow into wild populations conforms to historical patterns of gene flow in cotton (gossypium hirsutum) at its centre of origin.over 95% of the currently cultivated cotton was domesticated from gossypium hirsutum, which originated and diversified in mexico. demographic and genetic studies of this species at its centre of origin and diversification are lacking, although they are critical for cotton conservation and breeding. we investigated the actual and potential distribution of wild cotton populations, as well as the contribution of historical and recent gene flow in shaping cotton genetic diversity and structure. we e ...201121899621
indigenous perception and characterization of yanyanku and ikpiru: two functional additives for the fermentation of african locust bean.indigenous perception, processing methods, and physicochemical and microbiological characteristics of yanyanku and ikpiru, two additives used to produce fermented african locust bean condiments, sonru and iru, were evaluated. according to producers, these additives accelerate the fermentation and soften the texture of the condiments. yanyanku is produced by spontaneous fermentation with either hibiscus sabdariffa or gossypium hirsutum or adansonia digitata seeds, whereas only hibiscus sabdariffa ...201121888591
evidence of female-produced sex pheromone of adelphocoris suturalis (hemiptera: miridae): effect of age and time of day.after the reduction in the use of broad-spectrum insecticides, adelphocoris suturalis (hemiptera: miridae) has become an important pest of transgenic cotton, gossypium hirsutum l., in china. a. suturalis is a highly mobile insect, moving rapidly in and out of crop fields, and traps baited with sex pheromones may be useful in monitoring and controlling its numbers. however, little is currently known regarding the chemical communication based on sex pheromones by this species. to use the synthesiz ...201121882682
Agrobacterium-mediated virus-induced gene silencing assay in cotton.Cotton (Gossypium hirsutum) is one of the most important crops worldwide. Considerable efforts have been made on molecular breeding of new varieties. The large-scale gene functional analysis in cotton has been lagged behind most of the modern plant species, likely due to its large size of genome, gene duplication and polyploidy, long growth cycle and recalcitrance to genetic transformation(1). To facilitate high throughput functional genetic/genomic study in cotton, we attempt to develop rapid a ...201121876527
transcriptome-wide identification and stress properties of the 14-3-3 gene family in cotton (gossypium hirsutum l.).14-3-3s are a class of conserved proteins in eukaryotes involved in almost all cellular processes. published studies have shown that this family plays a role in response to stress conditions. in this study, comparative genomics identified thirty-one 14-3-3 cdnas encoding 25 unique proteins with transcripts that were sufficiently divergent in the coding regions to define different 14-3-3 members. phylogenetic analysis indicated that the cotton 14-3-3 family was similar to the family in the plant ...201121805362
activation of arabidopsis seed hair development by cotton fiber-related genes.each cotton fiber is a single-celled seed trichome or hair, and over 20,000 fibers may develop semi-synchronously on each seed. the molecular basis for seed hair development is unknown but is likely to share many similarities with leaf trichome development in arabidopsis. leaf trichome initiation in arabidopsis thaliana is activated by glabrous1 (gl1) that is negatively regulated by triptychon (try). using laser capture microdissection and microarray analysis, we found that many putative myb tra ...201121779324
a novel isoform of sucrose synthase is targeted to the cell wall during secondary cell wall synthesis in cotton fiber.sucrose (suc) synthase (sus) is the major enzyme of suc breakdown for cellulose biosynthesis in cotton (gossypium hirsutum) fiber, an important source of fiber for the textile industry. this study examines the tissue-specific expression, relative abundance, and temporal expression of various sus transcripts and proteins present in cotton. a novel isoform of sus (susc) is identified that is expressed at high levels during secondary cell wall synthesis in fiber and is present in the cell wall frac ...201121757635
qtl alleles for improved fiber quality from a wild hawaiian cotton, gossypium tomentosum.seventeen backcross-self families from crosses between two gossypium hirsutum recurrent parent lines (ca3084, ca3093) and g. tomentosum were used to identify quantitative trait loci (qtls) controlling fiber quality traits. a total of 28 qtls for fiber quality traits were identified (p < 0.001), including four for fiber elongation, eight for fiber fineness, four for fiber length, four for fiber strength, six for fiber uniformity, one for boll weight, and one for boll number. three statistically s ...201121735234
characterization of profilin genes from allotetraploid (gossypium hirsutum) cotton and its diploid progenitors and expression analysis in cotton genotypes differing in fiber characteristics.the actin-binding protein profilin (prf) plays an important role in cell growth and expansion by regulating the organization of the actin filaments. recent studies have reported association between fiber elongation in cultivated cotton (gossypium hirsutum) and prf expression. in the present study, we cloned four genomic clones from allotetraploid cotton (g. hirsutum) and its putative diploid progenitors (g. arboreum and g. raimondii) designated ghprf1_a, ghprf1_d, gaprf1, and grprf1 encoding cot ...201221725637
elucidation of thermotolerance diversity in cotton (gossypium hirsutum l.) using physio-molecular approaches.cotton (gossypium hirsutum) is an important cash crop, but high temperature during its growing season is one of the major factors that limit its productivity. this problem compels plant breeders to breed for heat tolerance, which can help to overcome this challenge. it is very important to make a comprehensive screening of heat-tolerant genotypes so that only the best are chosen. here we report the combined use of several techniques that can help breeders to screen their germplasm. twelve cultiv ...201121710467
cotton fibers can undergo cell division.ovular culture was used to determine the cell cycle aspects of cotton fiber cells. each ovule (gossypium hirsutum, cultivar, md51 ne) grown under the conditions used has ~10 000 fiber cells at 4 d postanthesis. about 25% of these cells divide when ovules are cultured at 34c. mitosis occurs after fiber cells differentiate, producing multicelled fibers. the basal and tip cells of multicelled fibers have the same characteristics as the polar ends of single-celled fibers. most cell division occurs i ...199721708678
growth and mitotic potential of multicelled fibers of cotton (malvaceae).we tested the hypothesis that the growth of multicelled cotton fibers of gossypium hirsutum, cultivar md51 ne, occurs exclusively within the tip cell. direct cellular measurements proved the hypothesis incorrect. the results show that all cells within a fiber grow and that the relative growth of the tip cell is reduced as the number of cells per fiber increases. also, measurements of two- and three-celled fibers show that the two daughter nuclei in two-celled fibers differ. the ability to divide ...199821684876
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