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[genital microbial flora in a population of high obstetrical risk].there is increasing evidence showing the association between maternal genital infection, prematurity and neonatal or maternal morbidity. to know the genital flora of women attending a high risk pregnancy service, 73 consecutive pregnant women attending the high risk pregnancy service at the hospital san borja-arriarán from may to august 1992 were included in this study, 75.3% of the vaginal discharges corresponded to fluids of normal appearance and 24.7% were altered. the microorganisms most fre ...19947809430
[gardnerella vaginalis biotypes: modification of a proposed system].a modified scheme is proposed for biotyping gardnerella vaginalis based on detection of hippurate hydrolysis, beta-galactosidase (onpg) and lipase, and fermentation of arabinose, galactose and xylose. thirty three biotypes were found among 140 strains from women with and without bacterial vaginosis (non-specific vaginitis). the distribution of biotypes were found to be significantly different, being more predominant the biotypes 1a; 5g; 7a; 7d and 7g in women with vaginosis and the biotypes 5g a ...19957784728
metronidazole during pregnancy. 19957781879
[bacterial vaginosis during chemotherapy for gynecological neoplasms. a therapeutic proposal].bacterial vaginosis (bv) is a well characterized disease, defined amsel's criteria, whose incidence is significantly increased during chemotherapy for gynecologic malignancies, as previously reported by the authors. nine patients out of 14 (64%) who were receiving adjuvant chemotherapy for gynecologic tumors showed a clinical and laboratory evidence of bacterial vaginosis and were treated with cetyltrimethilammonium naproxenate vaginal douches. results were analyzed up to the completion of oncol ...19957770150
the genital flora of women with intraamniotic infection. vaginal infection and prematurity study group.the relationship of genital flora assessed at the end of the second trimester of pregnancy and intraamniotic infection diagnosed by clinical signs and symptoms during labor was evaluated. women were enrolled at 23-26 weeks of gestation and followed through delivery in the multi-center vaginal infections and prematurity study (1984-1989). among the cohort of 11,989 followed through delivery, 286 (2.4%) developed intraamniotic infection. the recovery of gardnerella vaginalis (relative risk [rr] = ...19957769281
[gardnerella vaginalis infection in heterosexual couples. ultrastructural study of desquamation cells of the stratified epithelium].bacterial vaginosis is one of the most frequent infections during the woman's reproductive age. lactobacilli normal flora is substituted by relatively elevated gardnerella vaginalis (gv), anaerobic bacteroids, mobiluncus and mycoplasma. the purpose of this study is to perform a morphological analysis of possible mechanisms for adhesion and penetration of gv in the heterosexual couple, in squamous epithelium at vaginal wall, as well as in seminal fluid. ten couples with positive gv culture with t ...19957768469
studies on the anaerobic (bacterial) vaginosis among the bangladeshi women of reproductive age group.vaginal swab from 190 patients aged between 15 to 45 years with the complaint of excessive and foul smelling vaginal discharge and 50 healthy controls of comparable age were studied. different diagnostic techniques used for the detection of g.vaginalis were compared. antibiogram of isolated g.vaginalis was also performed to determine the drug sensitivity pattern. g. vaginalis were isolated from 74 patients (38.9%) and from 6 controls (12%). the rate of isolation of g. vaginalis in the vaginal sw ...19947748151
[a new zoonosis--investigation of gardnerella vaginalis disease of fox. ii. identification of pathogenic bacteria].16 strains isolated from aborted foetus and vaginal exeretions of foxes were used for examination of cell morphology including fine structure of the cell wall and septa, test for homolysis, biochemical reactions and analysis of g+c content of the bacterial dna. the cells are pleomorphic bacilli and coccobacilli, gram stain reaction appear positive to variable, catalse and oxidase-negative, with a fermentative type of glucose metabolism, giving acetic and lactic acid as the major end products of ...19957725680
[a new zoonosis--investigation of gardnerella vaginalis disease of fox. i. causative agent isolation and artificial infection].it is the first time that this paper reports on using improved rabbit blood agar culture medium to isolate 145 strains gardnerella vaginalis from the foxes and their abortion fortus organs of main fox farms. among the strains, 26 strains were isolated from the abortion foetus organs, isolation rate was 92.86%; 118 strains were isolated from the vaginal exeretion of the abortion and empty foxes, isolation rate was 34.01%; 1 strain was isolated from inguinal lymph node of the pelted positive fox, ...19957725679
[the isolation of streptococcus morbillorum from vaginal exudates].we have tested 195 vaginal secretions sent by gynecology service of this hospital between the years 1988-1990. we achieved positive culture for streptococci in 58 (30%) of these cultures, 26 (44.8%) corresponding to streptococcus morbillorum 9 (15.5%), to gardnerella vaginalis 5 (8.6%), to enterococcus faecalis-durans and to streptococcus agalactiae, 3 (5.1%) to streptococcus mitis and milleri 2 (3.4%), to streptococcus bovis and cremoris, and 1 (1.7%) to streptococcus salivarius, equinus and sa ...19957716323
lea's shield, a new barrier contraceptive preliminary clinical evaluations three-day tolerance study.in this study the lea's shield was evaluated for its tolerance by women who wore the device for three consecutive days. ten women who wore the lea's shield for 72 hours completed the tolerance study without adverse effects. examination of the cervix and vagina revealed that the device did not provoke any significant cellular or microbial changes among the wearers. no major changes in the appearance or prevalence of vaginal flora occurred in the women after three days of wearing the device. a gra ...19947705097
evaluation of the etest for antimicrobial spectrum and potency determinations of anaerobes associated with bacterial vaginosis and peritonitis.one hundred ninety-seven anaerobic organisms (24 gardnerella vaginalis, 16 mobiluncus spp., 19 peptostreptococcus spp., 20 lactobacillus spp., 20 prevotella bivia/disiens, 81 bacteroides fragilis group, 12 clostridium spp., and five fusobacterium spp.) were processed by the etest (ab biodisk, solna, sweden) and a reference (brucella blood agar) method against 10 antimicrobial agents. for the bacterial vaginosis-associated pathogens, the etest was more reproducible and correlated acceptably with ...19947705035
[a new zoonosis--investigation of gardnerella vaginalis disease of fox. iii. epidemiological investigation].by epidemiological investigation to gardnerella vaginalis disease of fox of civil main farms raising foxes, this disease was showed to be susceptible to silver foxes, arctic foxes, red foxes and color foxes, the disease was mostly transmitted by copulation, infected foxes played the leading role in epidemic of the disease. investigative results of all farms raising foxes showed infection rate of fox groups was 0.9-21.9%, resulting in abortion rate was 1.5-14.7%, empty rate was 3.2-47.5%. serious ...19957631502
comparative in-vitro activities of the new quinolone, bay y 3118, and ciprofloxacin, sparfloxacin, tosufloxacin, ci-960 and ci-990.the in-vitro activity of the new quinolone, bay y 3118, was compared with that of ciprofloxacin, tosufloxacin, sparfloxacin, ci-960 and ci-990 against 1640 isolates belonging to 117 bacterial species. against members of the enterobacteriaceae, bay y 3118 was as active as ci-960 and ci-990, up to four-fold more active than ciprofloxacin and tosufloxacin and up to 16-fold more active than sparfloxacin. the majority of enterobacteriaceae which were resistant to ciprofloxacin (mics > or = 2 mg/l) we ...19937605398
[perinatal infections. infection as etiological factor of preterm labor].on the ground of contemporary medical literature and our own observations, we have tried to estimate the participation of microorganism infection in preterm labor. in this article, we present mechanism, currently recognized as the most probable, that are leading to preterm labor under condition of perinatal infections. one of them seems to be the production of enzymes by pathological strains. those enzymes are considered to stimulate decidual and amniotic cells to arachidonic acid metabolism and ...19947597173
treatment of bacterial vaginosis: a comparison of oral metronidazole, metronidazole vaginal gel, and clindamycin vaginal cream.treatment options for bacterial vaginosis are numerous. the purpose of this study was to compare the efficacy of oral metronidazole, metronidazole vaginal gel, and clindamycin vaginal cream for the treatment of bacterial vaginosis using traditional clinical and laboratory methods, as well as a new dna probe test. we also determined the percentage of patients receiving each treatment who developed posttreatment vaginal candidiasis, a potential complication of treating bacterial vaginosis.19957595261
determination of gardnerella vaginalis genome size by pulsed-field gel electrophoresis.the chromosomal dna of four strains of gardnerella vaginalis were digested with rare cutting restriction enzymes and analyzed by pulsed-field gel electrophoresis (pfge). the four strains studied were two clinical isolates (gvp 004 & gvp 007) and two american type culture collection strains (atcc 14018 & atcc 14019). the restriction enzyme sfii generated two dna fragments of about 0.6 mb and 1.1 mb in all four strains giving a g. vaginalis genome size of about 1.7 mb. a similar genome size was ca ...19947584037
[bacteriological findings in patients with cervical intra-epithelial neoplasia].in order to evaluate the cervical flora in patients with histologically confirmed cervical intraepithelial neoplasia (grades i to iii) the microbiologic results of 216 patients with cin were compared with those of 100 symptom free controls. gardnerella vaginalis combined with mykoplasma spp. and bacteroides spp. were found in 22% of the patients with dysplasia and in 5% of the control group. the difference was significant (p < 0.004). microorganisms causing sexually transmitted diseases like nei ...19957571906
pcr primers and probes for the 16s rrna gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid.a set of broad-range pcr primers for the 16s rrna gene in bacteria were tested, along with three series of oligonucleotide probes to detect the pcr product. the first series of probes is broad in range and consists of a universal bacterial probe, a gram-positive probe, a bacteroides-flavobacterium probe, and two probes for other gram-negative species. the second series was designed to detect pcr products from seven major bacterial species or groups frequently causing meningitis: neisseria mening ...19947512093
bacterial vaginosis. 19957500074
office laboratory diagnosis of vaginitis. clinician-performed tests compared with a rapid nucleic acid hybridization test.the traditional diagnosis of vaginitis incorporates patient symptoms, clinical findings observed during vaginal examination, and laboratory analysis of vaginal fluid. the purpose of this study was to evaluate routine clinician-performed office laboratory diagnostic techniques for women with abnormal vaginal symptoms, and to compare these results with those obtained by a dna hybridization test for trichomonas vaginalis, gardnerella vaginalis, and candida species.19957500067
[a new zoonosis--investigation of gardnerella vaginalis disease of fox. iv. study of dot-elisa for the detection of serum antibody of gardnerella vaginalis disease of fox].192 gardnerella vaginalis antibody positive sera from artifically infected foxes, immunized foxes and foxes that the bacterial examination were positive were analysed by the established dot-elisa. the results showed that 187/192 were positive, the positive rate was 97.3%. block and cross tests showed that the method was specific. the method was 28.1 times sensitive as pat and 5.5 times sensitive as mat respectively. the repeatability of the test was good and the positive coincidence rate among t ...19957483585
[diagnosis and treatment of bacterial vaginosis by general practitioners].in order to evaluate which diagnostic procedures and treatments general practitioners (gp) used for bacterial vaginosis, and to describe their knowledge of the disease, all the gps in two counties were asked to fill in a questionnaire. two hundred and sixty-six (59%) answered. the gp used the criteria of amsel in 9%, the wet smear criteria in 12%, clue cells in 6%, the amine test in 30%, culture of gardnerella vaginalis in 29%, 8% only used clinical examination and 6% did not use any criteria. m ...19957483026
development of a species-specific polymerase chain reaction assay for gardnerella vaginalis.the nucleotide sequence of the region between the 16s and 23s rrna genes of the facultative anaerobic bacterium gardnerella vaginalis has been determined, together with the 5' proximal 500 nucleotides of the 23s rrna gene. regions suited for the development of specific, probe-confirmable polymerase chain reaction (pcr) assays were selected. pcr assays were evaluated with respect to sensitivity and specificity, the latter in comparison with a number of g. vaginalis reference strains and closely r ...19957477009
[on the taxonomy of actinobacillus, haemophilus, and pasteurella: dna base composition, respiratory quinones, and biochemical reactions of representative collection cultures (author's transl)].in a comparative study, 63 collection cultures representing 38 nomenspecies of, or assigned to, the genera actinobacillus, haemophilus, or pasteurella were characterized by phenotypical features and deoxyribonucleic acid base composition. the latter was calculated from the thermal denaturation point. biochemical reactions were tested in differential media commonly used for enterobacteriaceae, and two test procedures were compared: (i) pure cultures with haematin and nicotine adenine dinucleotide ...19807424243
current therapy of vulvovaginitis.trichomoniasis is reliably treated with a single 2-g dose of metronidazole; however, with this regimen simultaneous treatment of sexual partners is particularly important. trichomoniasis in pregnant women, who should not receive metronidazole, might be treated initially with clotrimazole vaginal suppositories, which appear to cure about 50% of cases. topical antifungal agents of the imidazole class are superior to polyenes in treating vulvovaginal candidiasis. boric acid powder applied intravagi ...19817330756
anaerobic and other fastidious microorganisms in asymptomatic bacteriuria in pregnant women.urine from 44 pregnant women with known or suspected renal disease (group 1) and from 50 healthy pregnant women (group 2) with negative routine cultures of midstream urine was obtained by suprapubic aspiration. aerobic and anaerobic cultures showed bacteriuria in 70% of the women in group 1 and in 26% of those in group 2. gardnerella vaginalis was the most frequently isolated organism. ureaplasma urealyticum was isolated from 14(48%) of 29 women in group 1 and from two (12.5%) of 16 women in gro ...19817276624
comparison of miconazole-coated tampons with clotrimazole vaginal tablets in the treatment of vaginal candidosis.the effectiveness and acceptability of miconazole-coated tampons were compared with those clotrimazole vaginal tablets in the treatment of vaginal candidosis in 100 women. both treatments were highly effective in reducing the signs and symptoms of infection; 95% of the group treated with miconazole had negative culture results for candida species immediately after treatment compared with 86% of those treated with clotrimazole. a 17.6% recurrence rate of positive culture results was found four we ...19817272707
[studies into bacterial gynecological infections, with particular reference to anaerobic and micro-aerophile germs (author's transl)].reported in this paper are 50 pyogenic infections, with anaerobic germs being cultured in 40,7 per cent of the cases, as well as 105 examinations of patients with flour, with haemophilus vaginalis having been isolated in 20 cases. widespread occurrence of such pathogens and severity of infections caused by such bacteria call for close cooperation between clinical practitioners and microbiologists, as no safe diagnosis is practicable without involvement of the latter. reference is made to possibl ...19817269879
corynebacterium vaginale in non-purulent vaginitis.the diagnostic importance of a cytological evaluation of a gram-stained smear of the discharge from a case of non-purulent vaginitis (nvp) due to corynebacterium vaginale is emphasized. a battery of rapid laboratory tests designed to identify the organism on the second day of incubation with an accuracy of at least 97% is proposed. in a prospective study of 1402 patients, 310 (22%) were found to be asymptomatic carriers of c. vaginale and 42 (3%) had vaginal discharge attributable only to c. vag ...19817254108
chlamydia trachomatis infections in women with urogenital symptoms.chlamydia trachomatis was isolated from 30 to 100 women attending a family physician's office with dysuria, frequency or vaginal discharge, compared with 2 of 30 asymptomatic women. multiple infections were common: c. trachomatis coexisted with gardnerella vaginalis, candida albicans, trichomonas vaginalis or a bacterial cause of urinary tract infection in 15 patients. c. trachomatis was isolated alone from 15 symptomatic women. the source of the positive culture was not always the site of sympt ...19827139448
[gardnerella vaginalis. a common cause of leukorrhea and colpitis?]. 19827123520
rapid microbiochemical method for identification of gardnerella (haemophilus) vaginalis.a rapid biochemical method for the identification of gardnerella vaginalis has been developed. the method is based on the fermentation of starch and raffinose and on the hydrolysis of hippurate. with this new procedure, identification was confirmed for 390 of 396 g. vaginalis isolates within 1 h after their inoculation into the three substrates.19827050164
aetiology and management of non-specific vaginitis.in a study of 100 women with non-specific vaginitis, characterised by a vaginal discharge which was malodorous and pruritic in most cases and caused dyspareunia and dysuria in some, gardnerella vaginalis was isolated in 46% of patients. when present, g vaginalis was significantly associated with mycoplasma hominis and bacteroides species. isolation of g vaginalis was unrelated to the presenting symptoms. treatment with povidone-iodine pessaries for two weeks produced no pronounced benefit, eithe ...19827034858
vaginitis associated with vaginal malodour.in two general practices in perth, western australia, the most common microbiological causes of vaginal discomfort in 368 patients were candida albicans. gardnerella (haemophilus) vaginalis, trichomonas vaginalis and bacteroides fragilis. amongst patients with abnormal vaginal odour, with or without vaginitis, the most common cause of odour was g. vaginalis. the writers advocate that heavy growths of group b streptococci, escherichia coli, and enterococci should be considered to be the possible ...19817029228
ureaplasma urealyticum in the upper urinary tracts of renal allograft recipients.urine samples from 123 renal transplant recipients were cultured for the presence of ureaplasma urealyticum and other fastidious microorganisms. ureaplasmas were recovered alone or in association with other microbial species from the bladder urine of 13 (11%) of the 123 patients, and evidence of involvement of the upper urinary tract was present in nine patients. colonization of the upper urinary tract by u. urealyticum was not associated with a decline in function of the grafted kidney and was ...19817024429
rapid identification of corynebacterium vaginale in non-purulent vaginitis.a simple set of tests is proposed to give excellent probability for the identification of corynebacterium vaginale from clinical material. using these tests, 380 c vaginale were isolated from genital tract specimens from 1402 patients. of these isolates 70 were from symptomatic patients. these 70 isolates were subjected to a further set of tests to confirm their identity. the advantage of these primary tests is that they can be completed on the day of isolation of the organism. of these 70 isola ...19817024317
ambulatory management of vaginal infections, infestations, and anomalies. 19817022512
[characterisation of corynebacterium vaginale by the fluorescent antibody technic (author's transl)].when using the serum of rabbits immunized against the reference vaginal strain 594 of gardner and dukes, the authors tried to find an antigenic identity between this strain and the usual strains that can ordinarily be detected in vaginal samples. the immunizing process that has been used is indirect immunofluorescence. through these studies, it is possible to conclude that, besides usual strains identical to the reference strain, some specific strains can be detected that have the same microscop ...19817018327
[possibilities for cultural diagnosis of vaginal flora (author's transl)].reported in this paper are some possible approaches to culturing of vaginal flora. plastic slides and various selective culturing media are described. the results so far achieved are likely to suggest that the above approach is applicable in practice.19817015753
gardnerella vaginalis perinephric abscess in a transplanted kidney. 19817010159
puerperal infections.this comprehensive review on puerperal infections covers risk factors, causative bacteria, pathophysiology, diagnosis, therapy of specific entities, and prevention. puerperal infection is problematic to define especially with antibiotics that change the course of fever. i may present as endometritis (most common), myometritis, parametritis, pelvic abscess, salpingitis, septic pelvic thrombophlebitis or septicemia, and also includes infections of the urinary tract, episiotomy, surgical wounds, ...19807004691
use of the rapid fermentation test in determining carbohydrate reactions of fastidious bacteria in clinical laboratories.the rapid fermentation test was used to determine the carbohydrate reactions of some of the fastidious bacteria encountered in clinical laboratories, such as: haemophilus species, including haemophilus vaginalis; actinobacillus actinomycetemcomitans; cardiobacterium hominis; kingella species; corynebacterium species; propionibacterium species; and erysipelothrix rhusiopathiae. results were usually obtained within 4 h by using inocula from 24- or 48-h blood or chocolate agar media.19806999028
treatment of vaginitis.the physiology and flora of the normal vagina and the more common causes and associated treatments of vaginitis are reviewed. vaginitis encompasses a group of diseases causing inflammatory changes in the vagina and vulva. treatment of the various vaginitides requires accurate diagnosis, which is hampered by difficulties in culturing pathogens and in resolving the pathogenicity of various organisms. the ability of the body to withstand vaginitis is affected by physiologic changes within the vagin ...19806996478
haemophilus vaginalis vaginitis after twenty-five years.this paper provides an abbreviated review of developments related to haemophilus vaginalis vaginitis since its original description a quarter of a century ago. my intervening years of interest and research in the field of vulvovaginal infections have served to further confirm the originally published concepts regarding this highly prevalent, precisely defined, readily recognizable, and aesthetically objectionable vaginal disease. an occasional investigator still questions the pathogenicity of th ...19806990764
cellular fatty acid composition of haemophilus species, pasteurella multocida, actinobacillus actinomycetemcomitans and haemophilus vaginalis (corynebacterium vaginale).the fatty acid composition of 35 haemophilus influenzae strains was found to be grossly similar and characterized by relatively large amounts of 14:0, 3-oh-14:0, 16:1 and 16:0. the three c18 fatty acids 18:2, 18:1 and 18:0 were also present, but in much lower concentrations. this general pattern was also found for most of the other species of haemophilus examined (h. aegyptius, h. aphrophilus, h. canis, h. gallinarum, h. haemolyticus, and h. parainfluenzae). small but distinct quantitative discr ...19806990689
the vaginal microbial flora in non-specific vaginitis.the facultative and strictly anaerobic vaginal microbial flora was investigated in 40 women with non-specific vaginitis and in 40 control women seen in private gynaecological practice. gardnerella vaginalis, anaerobic gram-negative bacilli, anaerobic gram-negative and gram-positive cocci were all associated with non-specific vaginitis (p less than 0.001), whereas lactobacilli occurred less frequently in non-specific vaginitis than in controls (p less than 0.01). the most common anaerobes were ve ...19826985215
comparison of microscopic and cultural findings in the diagnosis of gardnerella vaginalis infection.the diagnosis of gardnerella vaginalis infection on the basis of microscopic and cultural findings was compared. a total of 340 specimens of vaginal secretion were gram stained and plated on a medium selective for gardnerella vaginalis. positive culture was obtained in 165 cases. microscopy was unequivocally positive in 95, doubtful in 58 and negative in 187. positive microscopy was confirmed by culture in 99%. on the other hand, 21% of the negative microscopy results gave a false negative diagn ...19826985214
gardnerella vaginalis and non-specific vaginitis. introductory remarks. 19826985213
gardnerella vaginalis (hemophilus vaginalis), an unusual cause of peritonitis in c.a.p.d.gardnerella vaginalis is usually associated with nonspecific bacterial vaginitis. this paper presents a capd patiënt with peritonitis due to g. vaginalis. the infection route remained speculative. treatment with gentamicin was not successful while the clinical signs of peritonitis disappeared within 24 hours after starting cefazolin.19826985031
[treatment of gardnerella-vaginalis-associated colpitis with metronidazole]. 19826984793
neonatal cellulitis due to gardnerella vaginalis. 19826984180
gardnerella vaginalis and non-specific vaginitis. 19826983437
gardnerella vaginalis.this case commentary describes a common cause of vaginitis which is unfamiliar to many practising doctors because its status has been determined only in recent months. the commentary is based on a patient who presented to a melbourne general practice to which the student was assigned as part of the 'infections in the community' programme.19826983344
ureaplasma urealyticum in patients with acute symptoms of urinary tract infection. 19826981712
in vitro susceptibility of gardnerella vaginalis and bacteroides organisms, associated with nonspecific vaginitis, to sulfonamide preparations.recent reports suggest that anaerobic bacteroides organisms are frequently found with gardnerella vaginalis in nonspecific vaginitis. specimens taken from 96 women with vaginal discharge were tested simultaneously for these organisms. g. vaginalis was found in 73% of the specimens, bacteroides was found in 53%, and both organisms were found in 47%. sulfonamides have been widely used in the successful treatment of vaginitis. paradoxically, g. vaginalis is reported to be resistant, and it has been ...19826981374
[incidence and clinical importance of haemophilus vaginalis (corynebacterium vaginalis) (author's transl)].in 250 women aged between 16 and 55 years who came to a gynaecologist we looked for haemophilus vaginalis. in 9.6% it was possible to culture haemophilus vaginalis. the comparison of the differential vaginalis (v-)medium of greenwood with the starch medium of smith showed the much better resolution of the greenwood medium. in one-half of the patients with haemophilus vaginalis there was no clinical evidence of pathogenicity of the bacterium, whereas in the other half, there was only a slight sym ...19826980805
pharmacokinetics of metronidazole and its principal metabolites and their activity against gardnerella vaginalis.the hydroxy metabolite of metronidazole was found to be more active against 21 strains of gardnerella vaginalis than the parent compound and less affected by culture in carbon dioxide. after 400 mg oral metronidazole (flagyl) plasma concentrations of the two agents were below the minimum inhibitory concentrations (mics) for most g vaginalis strains tested. with 2 g metronidazole the plasma concentrations exceeded the mics of the more sensitive strains. even with the lower dose of metronidazole c ...19826980684
male carriage of gardnerella vaginalis. 19826980683
comparison of culture and microscopy in the diagnosis of gardnerella vaginalis infection.a comparison was made between human blood agar containing amphotericin b, nalidixic acid and either gentamicin or colistin for the isolation of gardnerella vaginalis from cases of non-specific vaginitis seen in a clinic for sexually transmitted diseases. the medium containing gentamicin was more inhibitory for non-gardnerella species, but not sufficiently inhibitory to allow direct plating in the clinic without spreading for single colonies. the diffuse beta haemolysis produced by g vaginalis on ...19826979558
method for isolation of gardnerella vaginalis (haemophilus vaginalis). characterization of isolates by gas chromatography.a method for the isolation of gardnerella vaginalis (haemophilus vaginalis) is presented. bacteria isolated from 48-hour cultures grown on human blood agar were identified by means of beta-hemolysis, colony morphology, sensitivity to antimicrobial agents, oxydase and catalase reactions. thirty-eight clinical isolates and one test strain were examined for fatty acid composition. hexadecanoic (16:0), octadecenoic (18:1) and octadecanoic (18:0) were the major fatty acids. also present, but in minor ...19826979160
treatment of hemophilus vaginalis vaginitis. 19826979017
balanoposthitis associated with gardnerella vaginalis infection in men.fourteen of 194 (7.2%) consecutive unselected men had positive culture results from genital swabs for gardnerella vaginalis. a higher yield of isolates was obtained from preputial (93%) than from urethral swabs (64%). of the 14 men, two had no detectable genital abnormality, eight non-gonococcal urethritis, and nine balanoposthitis. the urethral isolation rates for g vaginalis in men with and without non-gonococcal urethritis were not significantly different, but preputial isolation rates were s ...19826978164
controversies in the management of vaginitis. 19816976584
haemophilus vaginalis vaginitis (corynebacterium vaginale). 19816976323
gardnerella vaginalis vaginitis. 19816976122
the role of gardnerella vaginalis in nonspecific vaginitis. 19816975685
rapid presumptive identification of gardnerella vaginalis (haemophilus vaginalis) from human blood agar media.presumptive identification of gardnerella vaginalis from 48-h human blood agar cultures by using a gram stain, hemolysis, and colonial morphology was highly accurate.19816973571
non-specific vaginitis: its diagnosis and treatment. 19816973563
clinical features of vaginal infections. 19816973061
in vitro susceptibility of gardnerella vaginalis to high concentrations of sulfonamide compounds.the excipients of triple sulfa vaginal tablets were ineffective against gardnerella vaginalis in vitro. the three sulfonamides, however, were inhibitory, and minimal inhibitory concentration tests showed many strains to be susceptible to 25,000 mug of sulfacetamide per ml.19816972733
scanning electron microscopic examination of bacteroides fragilis and gardnerella vaginalis after exposure to concentration gradients of metronidazole and tinidazole.agar diffusion tests with metronidazole and tinidazole were performed with one strain each of bacteroides fragilis and gardnerella vaginalis (haemophilus vaginalis, corynebacterium vaginalis). their cell morphology was studied 'in situ' on agar surfaces by means of scanning electron microscopy (sem). a sharp growth end-point was found for b. fragilis, whereas with g. vaginalis there was a gradual decrease in the number and size of the colonies close to the agar well. the sem study also revealed ...19816972572
treatment of hemophilus vaginalis vaginitis.four antimicrobial agents (triple sulfa cream, doxycycline, ampicillin, and metronidazole) were studied by double-blind techniques to determine their effectiveness in the treatment of hemophilus vaginalis vaginitis, documented by vaginal culture in 96 patients. cure was confirmed by negative vaginal cultures 7 weeks after the start of therapy. metronidazole proved to be effective in 20 of 22 couples (90.9%) treated. sulfa cream, doxycycline, and ampicillin were effective in 47.8 to 63.6% of pati ...19816972018
a taxonomic study of gardnerella vaginalis (haemophilus vaginalis) gardner and dukes 1955.fifty-five strains received as haemophilus vaginalis or as catalase-negative coryneform bacteria from the vagina together with 61 marker cultures were subjected to numerical phenetic analyses using 149 unit characters. the data were examined using the simple matching (ssm), jaccard (sj) and pattern (dp) coefficients and clustering was achieved using the average linkage algorithm. cluster composition was not markedly affected by the coefficient used or by test error, estimated at 6 . 5%. the h. v ...19806971916
haemophilus vaginalis (corynebacterium vaginale, gardnerella vaginalis) in a family planning clinic population.vaginal specimens were obtained at 902 attendances from 522 women requiring vaginal examination at a family planning clinic. haemophilus vaginalis was found in 8% of specimens either by culture or by at least two out of three microscopical tests; lactobacilli were seldom found in the presence of the organism. increased signs and symptoms, especially an offensive odour, were found only when h vaginalis and staphylococcus were isolated together. the organism was found less often in patients using ...19816970605
hemophilus vaginalis vaginitis in children--two cases. 19806969784
metronidazole in treatment against haemophilus vaginalis (corynebacterium vaginale).the rate of bactericidal activity and inactivation of metronidazole was studied in time-kill curves with haemophilus vaginalis (corynebacterium vaginale). the minimum inhibitory concentrations of metronidazole for the eight strains tested ranged from 4 to 16 micrograms/ml. at a concentration of 20 micrograms/ml, metronidazole demonstrated a slow cidal effect against exponential-phase organisms, requiring 24 to 48 h for completion. inactivation of metronidazole during the time-kill curve was quit ...19806968175
the origin and diagnosis of "nonspecific vaginitis". 19806967563
anaerobic bacteria in nonspecific vaginitis.to study the cause of nonspecific vaginitis, we analyzed vaginal fluid from normal women and from 53 women with nonspecific vaginitis, using quantitative anaerobic cultures and gas-liquid chromatography for short-chained organic-acid metabolites of the microbial flora. in normal vaginal fluid, lactate was the predominant acid, and the predominant organisms were lactobacillus and streptococcus species (lactate producers). in nonspecific vaginitis, lactate was decreased, whereas succinate, acetate ...19806967562
hemophilus vaginalis bacteremia.during 1975--77 hemophilus vaginalis bacteremia occurred post partum in eight previously healthy women. seven had been admitted for delivery at term and one because of threatened abortion. six underwent cesarean section. post-partum pyrexia and neutrophilia were the main features. all the patients recovered uneventfully while receiving antibiotics. h. vaginalis is an infrequent agent of bacteremia; it affects predominantly women after obstetric trauma.19806966179
nonspecific vaginosis.nonspecific vaginosis (nsv) is a very common clinical syndrome with characteristic clinical, biochemical, and microbiologic features. there is a thin, malodorous homogeneous, grey, nonpurulent vaginal discharge. the discharge usually has a ph greater than 4.5, contains "clue cells" on wet mount examination, and produces a "fishy" odor when mixed with 10% potassium hydroxide. the discharge contains an increased concentration of at least seven amines which are presumably produced by bacterial deca ...19816941446
anaerobes in genitourinary infections in men.urethral and sub-preputial swabs from 150 men were examined. there was a strong association between the isolation of anaerobic bacteria, particularly bacteroides spp, and a clinical diagnosis of balanoposthitis, non-specific urethritis (nsu), or both. aerobic bacteria formed the predominant flora in 28 healthy controls whereas anaerobes were predominant in specimens from 79 patients with balanoposthitis, from 24 with nsu, and from 19 with both. bacteroides spp were the commonest isolates in all ...19836871653
identification of gardnerella (haemophilus) vaginalis.different tests for the identification of gardnerella (haemophilus) vaginalis and for its differentiation from catalase-negative unclassified coryneforms from the vagina were evaluated on over 200 bacterial strains, with special emphasis on optimal test conditions. a presumptive identification of g. vaginalis in the clinical laboratory can be made on the basis of colonial morphology, clear beta-hemolysis with diffuse edges on human blood bilayer-tween agar, a negative catalase test, and typical ...19826821205
the effect of a contraceptive vaginal ring and oral contraceptives on the vaginal flora.premenopausal women seeking a steroid contraceptive method were allowed to choose between a contraceptive vaginal ring (cvr) containing levonorgestrel and estradiol used in a 3-week in, 1-week out regimen (n=20) and an oral contraceptive (oc) containing levonorgestrel and ethinyl estradiol in a 28-day regimen (n = 10). cultures from the posterior vaginal fornix were obtained before therapy in both groups and monthly for 6 months for the cvr group and after 1, 3, and 6 months for the oc group. th ...19816797788
vaginitis revisited. 19816794847
vaginitis revisited. 19816794790
vaginitis revisited. 19816791731
adherence of bacteria to vaginal epithelial cells at various times in the menstrual cycle.adherence of vaginal isolates of escherichia coli, lactobacillus species, group b streptococci, gardnerella vaginalis and neisseria gonorrhoeae to exfoliated vaginal epithelial cells was studied in 10 healthy, sexually active medical students. studies were done pre- and postmenstrually and at midcycle for two consecutive menstrual cycles. the mean number of adherent bacteria per vaginal epithelial cell (range) was 3.4 (0 to 14) for e. coli, 60.5 (12 to 152) for lactobacillus species, 54.8 (21 to ...19816783548
sexually transmitted conditions among women college students.we studied 500 unselected young women who consulted a gynecologist in a student health service. most participants were symptom-free and had normal physical examinations. few sexually transmitted infections were encountered. neisseria gonorrhoeae was recovered from two and trichomonas vaginalis was obtained from 14 of 500 women. chlamydia trachomatis was recovered from 20 (4.6%) of 439 participants. genital warts, genital herpes, and molluscum contagiosum, respectively, were noted in seven, four, ...19816779634
relative susceptibilities of gardnerella vaginalis (haemophilus vaginalis), neisseria gonorrhoeae, and bacteroides fragilis to metronidazole and its two major metabolites.the susceptibilities of strains of gardnerella vaginalis (haemophilus vaginalis), neisseria gonorrhoeae, and bacteroides fragilis to metronidazole and its principal oxidative metabolites (1-[2-hydroxyethyl]-2-hydroxymethyl-5-nitroimidazole) ("hydroxy" metabolite) and 1-acetic acid-2-methyl-5-nitroimidazole ("acid" metabolite), were compared by determinations of the minimal inhibitory concentrations (mics) of these compounds. against ten strains of g. vaginalis, the hydroxy metabolite was the mos ...19806779387
inhibition of some normal and pathogenic vaginal bacteria by oral and vaginal streptococci.hemolytic and nonhemolytic strains of streptococcus faecalis, together with some strains of oral and vaginal alpha-hemolytic streptococci, produced one or more factors that inhibited the growth of gardnerella vaginalis, neisseria gonorrhoeae, and a variety of other vaginal bacteria. some streptococci were inhibitory to indicator organisms only in the presence of catalase, whereas catalase either enhanced or prevented inhibitory action for still other streptococci. the inhibitors produced by cert ...19806777883
vaginal discharge in the black pregnant patient: an evaluation of the relationship between symptomatology and diagnosis.three hundred and thirty pregnant black antenatal patients with symptomatic vaginal discharge were assessed. there appeared to be no correlation between the symptoms (pruritus, burning, amount of discharge and appearance) and the expected diagnosis. the common organisms trichomonas vaginalis, haemophilus vaginalis, candida albicans and neisseria gonorrhoeae were evaluated. it was also noted that a large number of patients (54%) had negative cultures although their complaints were similar to thos ...19806773152
selective differential human blood bilayer media for isolation of gardnerella (haemophilus) vaginalis.new selective and differential human blood bilayer agar media with tween 80 (hbt medium) or without tween 80 (hb medium), developed for the isolation of gardnerella (haemophilus) vaginalis, permitted significantly higher g. vaginalis isolation rates than have been obtained for other media used for this purpose. hb medium consists of a basal layer of columbia agar base containing colistin and naladixic acid with added amphotericin b and an overlayer of the same composition plus 5% human blood. hb ...19826764766
an overview of the diagnosis and treatment of gardnerella vaginalis and bacteroides associated vaginitis. 19826764201
the odour of non-specific vaginitis: a review. 19826764200
gardnerella vaginalis and its clinical syndrome.the gardnerella vaginalis syndrome is a well defined but benign condition characterized by a smelly vaginal discharge of ph greater than 5.0. it is not associated with inflammation. it often occurs in association with cervical infections. the microscopic appearance of the discharge is typical and diagnostic. the wet mount shows "clue cells" and "rafts" (floating clumps of small bacilli) and the gram stain has the "pepper on salt" pattern of masses of small gram-variable bacilli. the amine test i ...19826764199
identification of gardnerella vaginalis by a fluorescent antibody test. 19826762799
classic illustration. 19826761190
gardnerella vaginalis: laboratory isolation and clinical significance. 19826756626
mycoplasma hominis in nonspecific vaginitis.the prevalence of nonspecific vaginitis was 24% among 98 randomly selected women examined at a clinic for sexually transmitted diseases and 32% among 135 women with suspected cervicitis at the same clinic. mycoplasma hominis was isolated more often and serum antibody levels to m. hominis were higher in women with nonspecific vaginitis than in women without nonspecific vaginitis. similarly, the rate of isolation of gardnerella vaginalis and the quantitative growth of g. vaginalis were higher for ...19836689379
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