Publications

scanning electron microscopy of trachea and bronchi from gnotobiotic pigs inoculated with mycoplasma hyopneumoniae.	areas of trachea and right apical and right dorsal diaphragmatic bronchi from gnotobiotic neonatal pigs were examined by scanning electron and transmission electron microscopy at 7, 13, and 21 days after pigs were given intranasal inoculation of mycoplasma hyopneumoniae. similar areas were examined by scanning electron microscopy from 2 noninoculated neonatal pigs. tracheal and bronchial lesions were observed in all inoculated pigs. severe bronchial lesions were in grossly affected lobes. early ...	1977	911092
a fluorescent antibody technique for identification of mycoplasma hyopneumoniae colonies.	fluorescent antibody staining of mycoplasma hyopneumoniae colonies is difficult because the colonies wash from the agar. airdrying of the colonies grown on solid medium-overlayed glass microscope slides fixed the colonies in place, so that fluorescent antibody stain could be readily accomplished. apparent loss or alteration of antigenicity did not result from the air-drying process. the technique is useful for the identification of m hyopneumoniae isolates.	1976	773225
attempts to demonstrate cell-mediated immune response during mycoplasma suipneumoniae infection of pigs.	measurements of lymphocyte transformation and skin sensitivity were used to investigate whether pigs previously inoculated with mycoplasma suipneumoniae would show a t-lymphocyte-mediated immune response. in the lymphocyte transformation test blood lymphocytes, obtained at various intervals after intranasal inoculation of m suipneumoniae cultures, were incubated with specific antigen and the degree of transformation was assessed by 14c-thymidine uptake. reaction to intradermal antigen was assess ...	1978	749085
in vitro susceptibility of mycoplasma hyopneumoniae and mycoplasma hyorhinis to fifty-one antimicrobial agents.	by a tube dilution assay technique, 51 antimicrobial agents were singly tested against 9 strains of mycoplasma hyopneumoniae and 1 strain of m. hyorhinis for the purpose of obtaining information useful for selecting an agent for testing in vivo against porcine mycoplasmal pneumonia. based on determining minimal inhibitory concentrations and chemically grouping the agents into nine classes, all m. hyopneumoniae strains were found resistant to penicillins and peptides and susceptible to sulfonamid ...	1978	697348
cytopathogenicity of mycoplasma hyopneumoniae in porcine tracheal ring and lung explant organ cultures alone and in combination with monolayer cultures of fetal lung fibroblasts.	porcine tracheal rings and lung explants alone and in combination with monolayer cultures of porcine lung fibroblasts (plf) were separately inoculated with virulent strains of mycoplasma hyopneumoniae and incubated at various times. the preparations were observed by bright-field, phase-contrast, and scanning electron microscopy. in plf cultures, the strains at initial concentrations of 10(1.3) colony-forming units/ml increased within 3 days to 10(6) colony-forming units/ml, showed progressive cl ...	1978	669807
sensitivity in vitro to macrolide antibiotics and tetracyclines of mycoplasma hyopneumoniae isolated from pneumonic porcine lungs.		1978	652044
the immunological response of pig bronchial lymph node to mycoplasma suipneumoniae.		1978	621308
apparent re-infection of enzootic-pneumonia-free pig herds: specificity of diagnosis.	in a control scheme for enzootic-pneumonia-free herds, 43 herds developed enzootic pneumonia, as judged by non-specific clinical and pathological criteria over 10 years. material for cultural examination was obtained from 32 of these outbreaks, and mycoplasma suipneumoniae was identified in 28 of them. the four failures occurred before 1971 at a time when the cultural techniques were clearly inferior. since 1971 m suipneumoniae has been isolated from all the 17 outbreaks sampled for this purpose ...	1977	595285
isolation of mycoplasma hyopneumoniae from lesions in experimentally infected pigs.	pigs aged 6 to 9 weeks from enzootic pneumonia-free herds were inoculated intranasally with a suspension of pneumonic lung containing mycoplasma hyopneumoniae or were placed in contact with such inoculated pigs. all the inoculated pigs had gross lesions of enzootic pneumonia when killed 27 to 42 days after inoculation. the culture methods described enabled m. hyopneumoniae to be isolated from all 29 inoculated pigs. of 45 pigs in contact with inoculated pigs 35 had gross lesions of enzootic pneu ...	1979	533486
activity of tiamulin against mycoplasma suipneumoniae and enzootic pneumonia of pigs.		1979	452356
evaluation of diagnostic procedures for detection of mycoplasmal pneumonia of swine.	the complement-fixation test (cft), a latex-agglutination test (lat), and mycoplasma hyopneumoniae isolation procedures were compared with gross and microscopic lung evaluations for the routine diagnosis of mycoplasmal pneumonia (mp) of swine. the difficulty and variability of m hyopneumoniae isolation rendered this technique unacceptable. in market-weight swine, the finding of typical gross lesions was correlated with cft results (p less than 0.005). the mp status of a herd could not be determi ...	1979	447548
resistance of mycoplasma hyopneumoniae to chlortetracycline.		1979	435212
[studies on erythrocyte-rosette-forming peripheral blood cells in pigs infected with mycoplasma hyopneumoniae].		1979	420696
utilisation of glucose by mycoplasma suipneumoniae and mycoplasma flocculare.		1978	358810
quantitative detection of antibodies to mycoplasma suipneumoniae in pigs' sera by an ezyme-linked immunosorbent assay.	a new method for quantitative detection of antibodies to mycoplasma suipneumoniae in pigs' sera has been developed by the application of an enzyme-linked immunosorbent assay (elisa). the results show that the elisa is specific and highly sensitive. in experimentally infected pigs antibodies could be detected several weeks before the clinical manifestation of enzootic pneumonia.	1977	333714
mycoplasma suipneumoniae and mycoplasma flocculare in the growth precipitation test.		1977	327769
[immunological technics for the determination of mycoplasma suipneumoniae in frozen sections and bronchial smears].		1977	323968
influence of a mycoplasma respiratory tract infection on sheep erythrocyte and complement receptor-bearing cell composition in the respiratory tract of swine.	pulmonic and peripheral blood lymphocyte subpopulations in healthy and mycoplasma suipneumoniae-infected pigs were compared. the t- and the b-lymphocyte populations were counted, the b cells by the complement receptor (zymosan-complement rosettes) technique and the t cells by the ed-rosette technique (sheep rbc-dextran rosettes). the t cells were found to predominate among pulmonic, as well as blood, lymphocytes. pulmonic b-cell and blood t-cell percentages were increased after mycoplasma respir ...	1979	316662
microflora of pneumonic lungs in a pig herd established by hysterectomy.	mycoplasmas and other micro-organisms are recorded from a pig herd originally derived by hysterectomy. complement fixation tests were positive for mycoplasma hyopneumoniae 20 months after the first hysterectomy. following a marked increase in pneumonic lesions found at slaughter, and after modifying the culture medium m hyopneumoniae was isolated. the principal micro-organism associated with this mycoplasma was pasteurella multocida.	1976	130669