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[aquariums and mycobacterioses].mycobacterium infections are increasingly reported in fish fanciers who keep an aquarium. inoculation occurs on abraded skin after an incubation period of 2 to 3 weeks. clinically papulo-nodules, ulcers or verrucous plaques develop. these may progress into sporotrichoid lesions or into deeper infections involving tendons and bone. cultural identification is obtained by biopsy specimen at low temperature (30-33 degrees c). faster identification may be performed by polymerase chain reaction. histo ...200011068476
a case report of mycobacterium marinum infection of the hand.we report a case of mycobacterium marinum infection of the hand presenting initially as triggering of the digits. we like to highlight the unusual source of the infection and difficulty of diagnosis in this case as well as the various treatment modalities.200011063172
comparative genetic analysis of mycobacterium ulcerans and mycobacterium marinum reveals evidence of recent divergence.previous studies of the 16s rrna genes from mycobacterium ulcerans and mycobacterium marinum have suggested a very close genetic relationship between these species (99.6% identity). however, these organisms are phenotypically distinct and cause diseases with very different pathologies. to investigate this apparent paradox, we compared 3,306 nucleotides from the partial sequences of eight housekeeping and structural genes derived from 18 m. ulcerans strains and 22 m. marinum strains. this analysi ...200011053375
skin disease and nontuberculous atypical mycobacteria. 200011044189
applications of gene fusions to green fluorescent protein and flow cytometry to the study of bacterial gene expression in host cells. 200011036634
antibiotic susceptibility pattern of mycobacterium marinum.in vitro activities of 17 antibiotics against 53 clinical strains of mycobacterium marinum, an atypical mycobacterium responsible for cutaneous infections, were determined using the reference agar dilution method. rifampin and rifabutin were the most active drugs (mics at which 90% of the isolates tested were inhibited [mic(90)s], 0.5 and 0.6 microgram/ml, respectively). mics of minocycline (mic(90), 4 microgram/ml), doxycycline (mic(90), 16 microgram/ml), clarithromycin (mic(90), 4 microgram/ml ...200011036036
[cutaneous nodular infection with hemopathy].a 36-year-old man with treated hairy cell leukemia developed a skin infection due to mycobacterium marinum. a spectrum of atypical cutaneous mycobacteriosis presentations with immunosuppression can be found. the recognition of the disease needs cutaneous biopsies for histopathology and its identification by specific laboratory methods to adapt treatment.200011015652
incubation period and sources of exposure for cutaneous mycobacterium marinum infection: case report and review of the literature.the diagnosis of cutaneous mycobacterium marinum infection is often delayed for months after presentation, perhaps because important clinical clues in the patient's history are frequently overlooked. knowledge of the incubation period allows the clinician to target questions about the patient's history. prompted by a case with a prolonged incubation period, we sought to determine more precisely the incubation period of m. marinum infection. the medline database for the period 1966-1996 was searc ...200010987702
mycobacterium marinum hand infection: case reports and review of literature.three patients with mycobacterium marinum infection are described. key elements in the diagnosis of this infection are a high index of suspicion, taking a history with an emphasis on exposure to tropical fish or other potential sources of m. marinum infection, and tissue biopsy for culture and histology. the microbiologist should be informed about the suspicion of m. marinum infection so that appropriate cultures can be performed. as m. marinum does not grow under routine culture conditions, the ...200010878841
[sporotrichoid infection with mycobacterium marinum: successful therapy with oral tetracycline administration].atypical mycobacterial infections of the skin present a diagnostic and therapeutic challenge to dermatologists in many instances. we report on a patient who was diagnosed with atypical mycobacteriosis in its rarer, sporotrichoid form. possible differential diagnoses are discussed. efficient therapy using minocycline is demonstrated.200010875074
retrospective study of mycobacterium marinum skin infections.although infection by mycobacterium marinum is well reported in the literature, there have been few epidemiologic studies. the purpose of this study was to review the epidemiology of patients with cutaneous m. marinum infection over a 3-year period at the national skin centre in singapore.200010849123
new clues to how the tb bacillus persists. 200010847835
granuloma-specific expression of mycobacterium virulence proteins from the glycine-rich pe-pgrs family.pathogenic mycobacteria, including the agent of tuberculosis, mycobacterium tuberculosis, must replicate in macrophages for long-term persistence within their niche during chronic infection: organized collections of macrophages and lymphocytes called granulomas. we identified several genes preferentially expressed when mycobacterium marinum, the cause of fish and amphibian tuberculosis, resides in host granulomas and/or macrophages. two were homologs of m. tuberculosis pe/pe-pgrs genes, a family ...200010827956
mycobacteriosis in wild rabbitfish siganus rivulatus associated with cage farming in the gulf of eilat, red sea.infection patterns of mycobacterium marinum were studied over a period of 3 yr in wild rabbitfish siganus nivulatus populations associated with commercial mariculture cages and inhabiting various sites along the israeli red sea coastline. mycobacteriosis was first recorded from the red sea in 1990 in farmed sea bass dicentrarchus labrax and is absent from records of studies on parasites and diseases of wild rabbitfish carried out in the 1970s and 1980s. a sharp increase in the prevalence of the ...200010768289
cutaneous infections due to nontuberculous mycobacteria: histopathological review of 28 cases. comparative study between lesions observed in immunosuppressed patients and normal hosts.to evaluate the histopathological features observed in patients with cutaneous infections due to nontuberculous mycobacteria (ntm) and to compare the histopathological patterns observed in immunosuppressed patients and normal hosts. twenty-eight biopsy specimens corresponding to 27 patients with cutaneous infections due to ntm were reviewed. eighteen biopsies corresponded to normal hosts (14 mycobacterium marinum, 2 mycobacterium chelonae, 1 mycobacterium terrae and 1 mycobacterium gordonae) and ...200010728814
[cutaneous sporotricoid infection by mycobacterium marinum]. 200010721568
[long-standing skin nodular lesions in a 14-year-old male]. 199910687420
comparative severity of experimentally induced mycobacteriosis in striped bass morone saxatilis and hybrid tilapia oreochromis spp.twenty striped bass morone saxatilis and 20 hybrid tilapia oreochromis niloticus x o. mossambicus x o. aureus each received a single intramuscular injection of 1.6 x 10(6) colony forming units per gram body weight of mycobacterium marinum. striped bass manifested significantly greater clinical and microscopic disease compared to tilapia. whereas all the striped bass had died or were clinically ill by day 8 post-infection, there was no apparent disruption of normal behaviour, physical appearance, ...199910686670
transformation and transposition of the genome of mycobacterium marinum.to develop and evaluate protocols for genetic manipulations (transformation and transposition) of the fish pathogen, mycobacterium marinum.200010685681
analysis by enzyme-linked immunosorbent assay and immunoblot of the antibody responses of patients infected with mycobacterium marinum.the serum antibody responses of a total of 14 patients with active or recently cured mycobacterium marinum infections were analysed via a combination of enzyme-linked immunosorbent assay (elisa) and the immunodevelopment of western blots of m. marinum antigen. normal human sera and sera from patients with active pulmonary tuberculosis were also analysed as controls. the detectable igg response of m. marinum patients, as demonstrated by elisa, was highly variable and did not differ significantly ...200010683462
atypical mycobacterium infection with sporotrichoid spread in a patient with human immunodeficiency virus.a case of mycobacterium marinum infection presenting with a sporotrichoid spread in a hiv positive chinese male is presented. the patient responded to oral cotrimoxazole treatment. a brief review of the literature concerning atypical mycobacterial infection presenting in such a fashion is discussed.199910672400
[clinical presentation and therapy of mycobacterium marinum infection as seen in 12 cases].mycobacterium marinum (m.m.) is the causative pathogen of skin infections that have been called "swimming pool granulomas". an increasing number of reports that deep structures are involved in these infections was the reason for studying the clinical presentation and response of the infection to different therapeutic regimens.200010650818
mycobacterium marinum keratitis: pigmentation a clue to diagnosis. 199910624441
[sporotrichoid mycobacteriosis caused by mycobacterium marinum]. 199910572519
identification and semiquantitation of mycobacterium avium using a competitive pcr method.a competitive pcr method with standard dna (mimic) was developed for the rapid detection and semiquantitation of mycobacterium avium (m. avium) using primers specific for the alpha antigen sequence of the bacteria. dna from both m. avium and mycobacterium marinum was amplified by polymerase chain reaction (pcr), but only m. avium could be detected by subsequent blotting confirmation with a probe specific for the bacteria. with the pcr and subsequent dot blot hybridization, as little as 10 fg of ...199910553674
comparative antimycobacterial activities of ofloxacin, ciprofloxacin and grepafloxacin.infections caused by non-tuberculous mycobacteria and multidrug-resistant mycobacterium tuberculosis are difficult to treat. new compounds potentially active against these bacteria are therefore constantly being sought. among them is grepafloxacin, a new c5 fluoroquinolone. a panel of 130 isolates of mycobacteria including 33 m. tuberculosis isolates and 97 isolates of different species of atypical mycobacteria were analysed for susceptibility to grepafloxacin, ofloxacin and ciprofloxacin. the m ...199910552981
activation of human neutrophils by mycobacterial phenolic glycolipids.the interaction between mycobacterial phenolic glycolipids (pgls) and phagocytes was studied. human neutrophils were allowed to interact with each of four purified mycobacterial pgls and the neutrophil production of reactive oxygen metabolites was followed kinetically by luminol-/isoluminol-amplified chemiluminescence. the pgls from mycobacterium tuberculosis and mycobacterium kansasii, respectively, were shown to stimulate the production of oxygen metabolites, while pgls from mycobacterium mari ...199910540187
non-tuberculous mycobacterial tenosynovitis: a review.the clinical characteristics, outcome and treatment of non-tuberculous mycobacterial tenosynovitis are reviewed. from lesions localized in the hand, 10 different species of non-tuberculous mycobacteria have been reported. the most common are mycobacterium marinum and mycobacterium kansasii. other less frequent organisms are mycobacterium avium complex, mycobacterium szulgai, mycobacterium terrae, mycobacterium fortuitum, mycobacterium chelonae, mycobacterium abscessus, mycobacterium malmoense an ...199910482048
identification of mycobacterial dna in cutaneous lesions of sarcoidosis.sarcoidosis is a multisystemic granulomatous disease of uncertain etiology. recently, mycobacterial dna especially mycobacterium tuberculosis and mycobacterium avium complex were detected in lung tissue and bronchial lavage fluid from patients with sarcoidosis by polymerase chain reaction (pcr) assays in 30% to 50% cases. moreover, cell wall-defective form (cwdf) acid-fast bacteria have been isolated from skin lesions of patients with sarcoidosis which were later confirmed as m. avium complex by ...199910472755
recurrent, disseminated mycobacterium marinum infection caused by the same genotypically defined strain in an immunocompromised patient.an 81-year-old male with myasthenia gravis developed a cutaneous infection with mycobacterium marinum, which apparently resolved following local heat therapy. five months later, the patient developed new skin lesions and pancytopenia. m. marinum was isolated from his bone marrow. pulsed-field gel electrophoresis was performed to determine if the skin and bone marrow isolates were clonally related. digestion of the genomic dna with the restriction enzymes spei and asei yielded indistinguishable b ...199910449508
sporotrichoid cutaneous infection caused by mycobacterium marinum. 199910440450
[nontuberculous mycobacteria in the hospital environment].mycobacteria other than tubercle (mott) are ubiquitous. mycobacterial disease is most common at the extremes of age and at the patients with malignancy or hiv infection. in an attempt to establish effective measures to prevent nosocomial disease which has become increasingly important we investigated the presence of mott in hospital environment of oncological and pediatric units. between 1994-1997, 21 strains of mott were isolated from 500 environmental specimens (4.2% positive samples). the sou ...199810422317
a finger infection in a pet store employee. 199910414641
detection of mycobacteria in joint samples from patients with arthritis using a genus-specific polymerase chain reaction and sequence analysis.mycobacteria have been implicated in the pathogenesis of various forms of arthritis. the aim of this study was to examine the diagnostic potential of molecular biological techniques as well as to investigate the pathogenetic role of mycobacteria in chronic arthritis.199910402076
mycobacterium marinum infection in a renal transplant recipient.infections with atypical mycobacteria occur more frequently in patients with solid organ transplants than in the normal host.199910385095
superoxide production in phagocytes obtained from mycobacterium marinum-stimulated goldfish (carassius auratus) that were exposed to copper.to investigate the effects of copper exposure and recovery from copper toxicosis on the nonspecific immune response in mycobacterium marinum-inoculated goldfish.199910376889
mycobacterium marinum with associated bursitis.mycobacterium marinum infections have been reported for over 50 years, mostly in association with trauma in the setting of water exposure.199910366400
the mycobacterium marinum g13 promoter is a strong sigma 70-like promoter that is expressed in escherichia coli and mycobacteria species.a mycobacterium marinum promoter, designated g13, was isolated from a promoter-trap library as a constitutive producer of the mutant green fluorescent protein. sequence analysis, primer extension analysis, and computer promoter prediction analysis indicate that the g13 promoter is very similar to escherichia coli consensus sigma 70 promoters. expression of the green fluorescent protein from the g13 promoter in m. marinum is, however, up to 40 times higher than that seen from the mycobacterial hs ...199910361711
atypical mycobacterial cutaneous infection. 199910330598
health and safety concerns in fisheries and aquaculture.only 10% of all foodborne illnesses in the united states are attributed to seafood, making seafood a relatively safe food commodity. the implementation of hazard analysis and critical control points guidelines should make seafood consumption even safer. concerns include closed-loop, indoor, water-recirculating production systems; harmful algal blooms in marine environments, which can cause paralytic, neurologic, amnesic, and diarrhetic shellfish poisonings and ciguatera fish poisoning; bacteria ...199910329911
picture of the month. mycobacterium marinum facial abscess. 199910323638
pain and swelling along the nail fold of a 51-year-old man. 199810217741
fish tank granuloma: response to treatment with clarithromycin. 199910188167
polymerase chain reaction-reverse cross-blot hybridization assay in the diagnosis of sporotrichoid mycobacterium marinum infection.in this paper, we report a patient in whom mycobacterium marinum sporotrichoid infection was diagnosed using polymerase chain reaction (pcr) amplification of the 16s rrna gene and subsequent analysis of the amplified product in a reverse cross-blot hybridization assay with mycobacterial species-specific probes. this molecular method allowed us rapidly to detect and identify this organism directly in the patient's lesional skin biopsy rather than in cultures in conventional media. the identificat ...19989892957
atypical hand infections.the diagnosis and treatment of an atypical hand infection present a distinctive challenge for the hand surgeon. infections caused by these uncommon organisms occur more often in immunocompromised patients. this article emphasizes the salient features of mycobacterial, nocardial, and fungal infections of the hand. with an accurate and timely diagnosis, appropriate surgical and pharmacologic treatment may eradicate these unusual infections.19989884899
septic arthritis of the hand and wrist.septic arthritis of the hand and wrist is relatively uncommon. the most common cause is penetrating trauma such as a human or animal bite. the most common causative organism is staphylococcus aureus. septic arthritis caused by streptococcal species. haemophilus influenzae, pseudomonas aeruginosa, serratia species, neisseria gonorrhoeae, pasteurella multocida, eikenella corrodens, and mycobacterium marinum also may occur in specific clinical settings. the best clinical results occur following an ...19989884896
an unusual case of flexor sheath tenosynovitis.we report a case of a flexor sheath tenosynovitis caused by mycobacterium marinum.19989821624
[painful nodular lesions]. 19989793156
[mycobacterium marinum skin infections, apropos of a case]. 19989780437
the identification of mycobacterium marinum genes differentially expressed in macrophage phagosomes using promoter fusions to green fluorescent protein.mycobacterium marinum, like mycobacterium tuberculosis, is a slow-growing pathogenic mycobacteria that is able to survive and replicate in macrophages. using the promoter-capture vector pfpv27, we have constructed a library of 200-1000 bp fragments of m. marinum genomic dna inserted upstream of a promoterless green fluorescent protein (gfp) gene. only those plasmids that contain an active promoter will express gfp. macrophages were infected with this fusion library, and phagosomes containing flu ...19989767585
mycobacterium marinum: chronic and extensive infections of the lower limbs in south pacific islanders.we report three adult cases of very chronic, extensive infection of the lower limbs due to mycobacterium marinum. the patients were from south pacific islands and, clinically, the widespread warty plaques resembled chromomycosis. one was associated with severe lymphoedema. all three patients gave a history of at least 20 years duration. the patients were otherwise well and not immunologically compromised. in all cases, the organism was identified on tissue cultures and was not seen on histopatho ...19989737045
oxidative stress response and characterization of the oxyr-ahpc and fura-katg loci in mycobacterium marinum.oxidative stress response in pathogenic mycobacteria is believed to be of significance for host-pathogen interactions at various stages of infection. it also plays a role in determining the intrinsic susceptibility to isoniazid in mycobacterial species. in this work, we characterized the oxyr-ahpc and fura-katg loci in the nontuberculous pathogen mycobacterium marinum. in contrast to mycobacterium smegmatis and like mycobacterium tuberculosis and mycobacterium leprae, m. marinum was shown to pos ...19989733688
electron microscope studies of the in vitro phagocytosis of mycobacterium spp. by rainbow trout oncorhynchus mykiss head kidney macrophages.the cytological response of rainbow trout oncorhynchus mykiss head kidney macrophages to ingested mycobacterium spp. was examined in vitro. mycobacterium marinum or mycobacterium sp. tb267 isolated from snakehead fish channa striata bloch were opsonised with either fresh rainbow trout serum, serum which had been heat-inactivated, or rainbow trout antiserum against the extracellular products (ecp) of the 2 mycobacterium spp. a monoclonal antibody against the ecp was also used as an opsonin. suspe ...19989676251
mycobacterium marinum wrist arthritis: local and systematic dissemination caused by concomitant immunosuppressive therapy.the diagnostic difficulties and the potential serious course of mycobacterium marinum synovitis are illustrated in a patient in whom treatment with local steroid injections and immunosuppressive therapy resulted in local destruction of the wrist and systemic reactions. healing was achieved after correct medical and surgical treatment was initiated.19989670365
concurrent infection due to shewanella putrefaciens and mycobacterium marinum acquired at the beach.infection with marine bacteria is uncommon. a patient with systemic lupus erythematosus who developed concurrent infection with shewanella putrefaciens and mycobacterium marinum (m. marinum) is described. after bathing leg ulcers in sea water, severe cellulitis of the left leg with necrotic areas and extensive bullae developed. infection due to s. putrefaciens was confirmed and a long course of hospitalization, oral ciprofloxacin and skin grafting was required. during hospitalization subcutaneou ...19989611378
goldfish, carassius auratus, a novel animal model for the study of mycobacterium marinum pathogenesis.we have developed an animal model for studying mycobacterial pathogenesis using mycobacterium marinum and the goldfish, carassius auratus. goldfish are injected intraperitoneally with doses between 10(2) and 10(9) cfu of m. marinum organisms. depending on the dose of m. marinum organisms administered, an acute or chronic disease is produced. the acute disease is characterized by systemic mycobacterial infection, severe peritonitis, tissue necrosis, and a short median survival time. the chronic d ...19989596770
differentiation of mycobacterium ulcerans, m. marinum, and m. haemophilum: mapping of their relationships to m. tuberculosis by fatty acid profile analysis, dna-dna hybridization, and 16s rrna gene sequence analysis.although mycobacterium ulcerans, m. marinum, and m. haemophilum are closely related, their exact taxonomic placements have not been determined. we performed gas chromatography of fatty acids and alcohols, as well as dna-dna hybridization and 16s rrna gene sequence analysis, to clarify their relationships to each other and to m. tuberculosis. m. ulcerans and m. marinum were most closely related to one another, and each displayed very strong genetic affinities to m. tuberculosis; they are actually ...19989542909
mycobacterial phagosome maturation, rab proteins, and intracellular trafficking.one of the most prominent features of pathogenic mycobacteria, which include the potent human pathogens mycobacterium tuberculosis and mycobacterium leprae and their opportunistic relatives mycobacterium avium and mycobacterium marinum, is their ability to survive and multiply in phagosomes of mononuclear phagocytic cells. the phagocytosed mycobacteria reside in a vacuolar compartment which is exempted from maturation into the phagolysosome. recently, the arrest of the maturation of phagosomes c ...19979527483
verrucous nodule of the finger. 19989521036
inteins in mycobacterial gyra are a taxonomic character.the a subunit of dna gyrase in mycobacteria is frequently subjected to splicing events as its gene, gyra, harbours an insertion encoding an intein. investigation of a number of different isolates of mycobacterium kansasii, mycobacterium malmoense, mycobacterium marinum, mycobacterium ulcerans and mycobacterium xenopi demonstrated that the presence of gyra inteins is not random but a taxonomic character specific for a given taxon at a species or subspecies level.19989493395
identification of mycobacteria infecting fish to the species level using polymerase chain reaction and restriction enzyme analysis.an assay is described utilizing pcr technology for a rapid diagnostic test to identify fish infection with mycobacterium marinum, m. fortuitum and m. chelonae. a 924 bp dna fragment from a highly conserved area of the mycobacterial 16s rrna gene was amplified using mycobacteria genus-specific primers and digested with restriction enzymes (bani and apai). this examination yielded unique restriction patterns for each mycobacterial specie enabling identification of mycobacteria infecting fish to th ...19979453133
primary intravascular lymphomatosis associated with mycobacterium marinum.intravascular lymphomatosis (i.v.l.) is a rare condition in which neoplastic cells preferentially infiltrate blood vessels of the central nervous system. nonspecific symptoms associated with i.v.l. include dementia, seizures, and multifocal cerebrovascular events. i.v.l. was discovered at autopsy of a patient whose neurological deficits were predated by a particularly aggressive form of mycobacterium marinum soft-tissue infection. it is speculated that i.v.l. may have had an occult effect on the ...19989442593
fish tank granuloma--an unusual cause of skin infection. 19979440925
fish tank granuloma--a frequently misdiagnosed infection of the upper limb.five patients attended the accident and emergency (a&e) department with fish tank granuloma caused by an infection with mycobacterium marinum. all patients had forearm symptoms which were initially misdiagnosed. they were later recognised by the presence of superficial cutaneous lesions in a sporotrichotic distribution. definitive diagnosis was confirmed by the histological appearances of a biopsy and or culture of the organism. all patients responded to oral minocycline and had uncomplicated re ...19979413782
[persistent tuberous plaque in a finger]. 19979411574
osteomyelitis and tenosynovitis due to mycobacterium marinum in a fish dealer.osteomyelitis caused by nontuberculous mycobacteria is rarely reported. we describe a case of tenosynovitis and osteomyelitis of the right middle finger and metacarpal bone caused by mycobacterium marinum in a fish dealer. this 52-year-old woman suffered progressive pain, numbness, tenderness, and erythematous swelling of the right middle finger over a 2-month period. a radiograph of the right hand disclosed osteolytic lesions at the third metacarpal bone and the third proximal phalanx. she was ...19979409126
mycobacterium marinum infection causing septic arthritis and osteomyelitis.a 48-yr-old female on immunosuppressive therapy for fibrosing alveolitis and polymyositis developed a septic arthritis of the left middle finger proximal interphalangeal joint, tenosynovitis of the left palm and osteomyelitis of the right hindfoot due to infection with mycobacterium marinum. such widespread and severe bone and joint involvement has not been described previously with this organism.19979402866
direct detection of mycobacterium tuberculosis complex in clinical samples from patients in norway by ligase chain reaction.our aim was to investigate the use of dna amplification with the ligase chain reaction (lcr) for detection of the mycobacterium tuberculosis complex directly in human clinical specimens. the lcr assay employed was the abbott lcx mtb assay, which uses the gene encoding protein antigen b as the target template. four hundred eighty-two samples from 457 patients in one clinical microbiology laboratory in norway were processed by routine culture analysis (bactec culture), direct microscopy (ziehl-nee ...19979399528
[mycobacterium marinum. a rare cause of infection of the skin and joints].mycobacterium marinum is a rare cause of disseminated infection in man. the case report describes an 80-year-old woman, who had been treated with oral corticosteroids for bronchial asthma for 40 years, and in the same period had been swimming daily in swimming pools. at the first admission, the symptoms and clinical findings were interpreted as seronegative rheumatoid arthritis. after eight years of disease with recurrent infections of the skin, periarticular tissues and joints in the hands and ...19979304271
a crtb homolog essential for photochromogenicity in mycobacterium marinum: isolation, characterization, and gene disruption via homologous recombination.a gene essential for light-induced pigment production was isolated from the photochromogen mycobacterium marinum by heterologous complementation of an m. marinum cosmid library in the nonchromogen mycobacterium smegmatis. this gene is part of an operon and homologous to the streptomyces griseus and myxococcus xanthus crtb genes encoding phytoene synthase. gene replacement at this locus was achieved via homologous recombination, demonstrating that its expression is essential for photochromogenici ...19979294446
images in clinical medicine. mycobacterium marinum infection of the hand. 19979271484
mycobacterium marinum skin infection successfully treated with levofloxacin. 19979267238
[mycobacterium marinum as the rare cause of a chronic skin and soft-tissue infection]. 19979264926
application of the etest to the antimicrobial susceptibility testing of mycobacterium marinum clinical isolates.mycobacterium marinum, a well-recognized cutaneous pathogen, is usually treated by chemotherapy without available standardized in vitro susceptibility testing information. in this study, we have attempted to apply the stable-gradient method (etest; ab biodisk, solna, sweden) to susceptibility testing of m. marinum in order to assess the activities of eight antimicrobial agents against 60 recent clinical strains of m. marinum collected from 10 geographic sites within the united states. two plated ...19979230386
fish tank granuloma in a 14-month-old girl.a 14-month-old girl developed a persistent ulcerated nodule on her right lower leg associated with further nodules along the thigh. a clinical diagnosis of fish tank granuloma was suspected because of tropical fish tanks at home. the diagnosis was confirmed when mycobacterium marinum was isolated from low-temperature culture of skin tissue. the child made a complete recovery following treatment with rifampicin for 6 months despite in vitro sensitivity tests reporting resistance. m. marinum infec ...19979192414
differential trafficking of live and dead mycobacterium marinum organisms in macrophages.we characterized the mycobacterium marinum phagosome by using a variety of endocytic markers to follow the path of the bacteria through a mouse macrophage cell line. using a laser confocal microscope, we found that the majority of viable m. marinum cells were in nonacidic vacuoles that did not colocalize with the vacuolar proton atpase (v-atpase), the calcium-independent mannose-6-phosphate receptor (ci-m6pr), or cathepsin d. in contrast, heat-killed organisms and latex beads were in acidic vacu ...19979119492
direct detection and identification of mycobacterium ulcerans in clinical specimens by pcr and oligonucleotide-specific capture plate hybridization.we compared various diagnostic tests for their abilities to detect mycobacterium ulcerans infection in specimens from patients with clinically active disease. specimens from 10 patients from the area of zangnanado (department of zou, benin) with advanced, ulcerated active m. ulcerans infections were studied by direct smear, histopathology, culture, pcr, and oligonucleotide-specific capture plate hybridization (oscph). a total of 27 specimens, including 12 swabs of exudate collected before debrid ...19979114387
[cutaneous infections by mycobacterium marinum. description of 3 cases and review of the literature].human infection by mycobacterium marinum is infrequent and is currently reported in association with the management of aquariums. the epidemiologic, clinical and microbiologic characteristics of three cases in our hospital are herein reported.19979101751
antimicrobial treatment of fish tank granuloma.three patients with fish tank granuloma of the hand and forearm are reported. each patient was treated with antimicrobial regimes which have rarely or never been previously used in this condition. two patients responded well to treatment, one who received ciprofloxacin plus clarithromycin and another who was given clarithromycin plus ethambutol. the third patient received six different antimicrobial regimes before responding to a combination of rifabutin and ciprofloxacin. our experience suggest ...19979061551
facial sporotrichoid infection with mycobacterium marinum.we report a case of mycobacterium marinum facial sporotrichoid infection in an otherwise healthy 2-year-old child, probably acquired through contact with pets in an aquarium. the m. marinum isolate was susceptible to clarithromycin, and the child was successfully treated with oral antibiotic therapy. this unusual case emphasizes the importance of a thorough history in the evaluation of a patient with chronic sporotrichoid skin lesions.19979042142
[a case of arthritis of the wrist caused by mycobacterium marinum. review of the literature].the authors report the case of a woman aquarium keeper who developed septic arthritis of her right wrist due to mycobacterium marinum. clinical status and history, histopathologic and microbiologic characteristics are reviewed and discussed. the authors emphasize the importance of urgent and aggressive surgery combined with antimicrobial chemotherapy.19969026056
mycobacterium marinum causes both long-term subclinical infection and acute disease in the leopard frog (rana pipiens).mycobacterium marinum grows at an optimal temperature of 33 degrees c, far lower than that for m. tuberculosis. consequently, m. marinum infection of mammals is restricted largely to the cooler surfaces of the body, such as the extremities, but it causes a systemic infection in a large number of poikilothermic animals. here, we describe a laboratory animal model for m. marinum disease in the leopard frog (rana pipiens), a natural host species. m. marinum causes a chronic granulomatous, nonlethal ...19979009340
in vitro & in vivo experimental susceptibility of mycobacterium marinum to augmentin.the effect of augmentin alone and in combination with various beta-lactam antibiotics was studied against a pathogenic mycobacterium, m. marinum. the in vitro studies did not reveal any additional advantage over that found with augmentin alone and this antibiotic seemed considerably inhibitory to m. marinum at < 1 microgram/ml concentration. in vivo, the effects of augmentin on experimentally produced lesions in the mouse foot pads (mfps) showed a significant regression of the lesions, which was ...19968979517
[mycobacterium marinum skin infection in spain]. 19968948935
comparative activity of azithromycin against clinical isolates of mycobacteria.azithromycin exhibited in-vitro activity against 20 clinical isolates of mycobacterium avium complex for which the mic90 was 32 mg/l and 22 clinical isolates of other mycobacteria but showed no activity against 20 isolates of mycobacterium tuberculosis (mic90 > 128 mg/l) nor against the single isolate of mycobacterium marinum tested (mic 128 mg/l). these results suggest that the drug may prove useful for the prophylaxis and treatment of infections due to non-tuberculous mycobacteria, including m ...19968889727
infection of the upper extremity by mycobacterium marinum in a 3-year-old boy--diagnosis by 16s-rdna analysis.a 3-year-old boy developed several subcutaneous nodular lesions on his right arm. based on the histological examination of one of these nodules furunculosis was suspected and cefuroxime was tentatively given. however, acid-fast bacilli were then detected in the tissue specimen and a few colonies of acid fast, gram-positive rods grew on blood agar. definitive species diagnosis (mycobacterium marinum) was rapidly achieved by automated sequencing of amplified 16s-rdna and antimicrobial therapy was ...19968852464
mycobacterium marinum infection of the finger.a case of mycobacterium marinum infection of a finger is described. this is a rare condition in the uk. the relevant literature is reviewed.19958770745
applications for green fluorescent protein (gfp) in the study of host-pathogen interactions.the green fluorescent protein (gfp) from aequorea victoria is a novel fluorescent marker that has potential use in the study of bacterial pathogenicity. to explore some of the potential applications of gfp to the study of host-parasite interactions, we constructed two gfp expression vectors suitable for different facultative intracellular bacterial pathogens. the first expression vector was tested in the enteric pathogens, salmonella typhimurium and yersinia pseudotuberculosis, and the second ve ...19968707055
[abscessed skin lesions caused by mycobacterium marinum]. 19968695693
disseminated mycobacterium marinum infection and bacteremia in a child with severe combined immunodeficiency.we report an unusual case of disseminated dermatitis, osteomyelitis, and bacteremia in an immunocompromised host. an infant presented with a pustular skin rash resembling chicken pox, but culture of a skin lesion yielded mycobacterium marinum. upon further evaluation, severe combined immunodeficiency was diagnosed. radiographs of the hands and feet showed evidence of osteomyelitis. m. marinum was isolated from blood and synovial fluid. we recommend a high level of suspicion for this organism in ...19958589169
mycobacterium marinum disease in anne arundel county: 1995 update.a retrospective survey of 41 cases of culture-positive mycobacterium marinum disease in anne arundel county, maryland, showed that most infection was related to recreational exposure to the chesapeake bay and its tributaries. three quarters of cases consisted of skin or joint/tendon infection of the upper extremity, particularly the hand. an empiric drug regimen for a granulomatous soft tissue infection in this context should include rifampin and ethambutol or cotrimoxazole (trimethoprim/sulfame ...19958544670
right hand and arm rash mycobacterium marinum infection. 19958542494
mycobacterium marinum. 19958521369
nodular lymphangitis: a distinctive but often unrecognized syndrome.to describe nodular lymphangitis by reviewing the clinical and epidemiologic features of this disease with an emphasis on distinguishing specific etiologic agents.19938480962
mycobacterium marinum skin infections: two case reports.most infections of soft tissue injury sites are caused by staphylococcus or streptococcus and respond to beta-lactam antibiotics. occasionally, a patient does not respond to routine antibiotics, and other possibilities must be considered. a detailed history of the event that caused the soft tissue injury can be important in the diagnosis. two cases of mycobacterium marinum soft tissue infection (ie, fish tank granuloma) are presented.19938454982
mycobacterium marinum cutaneous infections acquired from occupations and hobbies.non-tuberculous mycobacterial (ntb) infections are not commonly diagnosed in thailand. the dissertation of reported cases among 10 published reports of 44 cases within 20 years revealed only six cases of cutaneous infections in which m. marinum was not included.19938340185
[aquarium-borne mycobacterium marinum infection].three cases of cutaneous mycobacterium marinum infection are described. all these patients had most probably been infected from home aquariums. in one of the patients, m. marinum was isolated by culturing a biopsy specimen from a lesion. clinical remission was achieved by means of antituberculous triple therapy, trimethoprim-sulfonamide, and no treatment, respectively.19938337642
[two cases of acquired immunodeficiency syndrome with disseminated non-tuberculous mycobacterial infection].two cases of acquired immune deficiency syndrome (aids) with disseminated non-tuberculous mycobacterial infection are reported. both patients had hemophilia and were infected with human immunodeficiency virus type 1 (hiv) by antihemophilic factor infusion. in case 1, a 44-year-old male, mycobacterium marinum, which ordinarily causes cutaneous infection, was isolated from sputum before death and from the lung, spleen, bone marrow, liver and lymph node at autopsy. this is the first report of disse ...19938294774
detection and identification of a pathogenic marine mycobacterium from the european seabass dicentrarchus labrax using polymerase chain reaction and direct sequencing of 16s rdna sequences.mycobacteriosis has become a major concern for the commercial mariculture of the european sea bass dicentrachus labrax in israel. the disease remains asymptomatic for a long time, is virtually impossible to eradicate with antibiotics, stunts the growth of the fish and renders the fish unmarketable. the pathogen was identified as mycobacterium marinum by direct sequencing and analysis of approximately 600 bp of the pathogen ribosomal encoding dna (rdna). the polymerase chain reaction technique wa ...19938293073
in-vitro activities of quinolones against mycobacteria.from the results of recently-published in-vitro studies, we have reviewed the activities of novel quinolones which are currently available, either commercially or for investigative purposes, against mycobacterium tuberculosis, the atypical mycobacteria (principally the mycobacterium avium complex, mycobacterium chelonae, mycobacterium xenopi, mycobacterium marinum and mycobacterium fortuitum) and mycobacterium leprae. we have also evaluated the effects of the various methods for determining the ...19938144420
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