Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [identification of hexavalent chromium reducing bacteria cr4-1 and optimization of its reduction conditions]. | to identify the hexavalent chromium reduction cr4-1, and to study the better conditions of the bacterial growth and its cr(vi) reduction. | 2015 | 25997219 |
| diversity of food-borne bacillus volatile compounds and influence on fungal growth. | to evaluate the antifungal activity of the volatile organic compounds (vocs) produced by 75 different food-borne bacillus species against aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus clavatus, fusarium oxysporum f. sp. lactucae and moniliophthora perniciosa and to determine the vocs responsible for the inhibition. | 2015 | 25989039 |
| involvement of the cask/r two-component system in optimal unsaturation of the bacillus cereus fatty acids during low-temperature growth. | bacillus cereus sensu lato is composed of a set of ubiquitous strains including human pathogens that can survive a range of food processing conditions, grow in refrigerated food, and sometimes cause food poisoning. we previously identified the two-component system cask/r that plays a key role in cold adaptation. to better understand the cask/r-controlled mechanisms that support low-temperature adaptation, we performed a transcriptomic analysis on the atcc 14579 strain and its isogenic ∆cask/r mu ... | 2015 | 25987542 |
| chromosome-directed pcr-based detection and quantification of bacillus cereus group members with focus on b. thuringiensis serovar israelensis active against nematoceran larvae. | bacillus thuringiensis serovar israelensis is a wide-spread soil bacterium affiliated with the b. cereus group (bcg) and is widely used in biocontrol products applied against mosquito and black fly larvae. for monitoring and quantification of applied b. thuringiensis serovar israelensis and its effect on indigenous b. thuringiensis serovar israelensis and bcg assemblages, efficient and reliable tools are essential. the abundance and properties of b. thuringiensis serovar israelensis strains in t ... | 2015 | 25979887 |
| a novel antimicrobial flavonoid from the stem bark of commiphora pedunculata (kotschy & peyr.) engl. | a new flavonoid, 2-(3,5-dihydroxy-4-methoxy-phenyl)-3,5-dihydroxy-8,8-dimethyl-2,3-dihydro-8h-pyrano[3,2]chromen-4-one, together with previously reported epicatechin was isolated from the ethyl acetate soluble fraction of the methanol extract of the stem bark of commiphora pedunculata. the structures of these compounds were elucidated based on extensive analysis of their spectral data, including 1 and 2d nmr. the compounds were active against 9 out of 12 tested microorganisms including a resista ... | 2016 | 25978097 |
| in vitro antibacterial activities and mechanism of sugar fatty acid esters against five food-related bacteria. | the objective of this study was to evaluate the antibacterial activities of sugar fatty acid esters, with different fatty acid and saccharide moieties, against five food-related bacteria including bacillus cereus, bacillus subtilis, staphylococcus aureus, escherichia coli and salmonella typhimurium. sucrose monocaprate showed the strongest antibacterial activity against all tested bacteria, especially gram-positive bacteria. the minimum inhibitory concentrations (mics) for gram-positive bacteria ... | 2015 | 25977039 |
| assessment of the microbial load of some medicinal plants commonly used in romania. | this study aims to assess the microbial load of some medicinal plants (matricaria chamomilla, achillea millefolium, ocimum basilicum, calendula officinalis, tilia cordata, hypericum perforatum) commonly used as medicinal teas. | 2015 | 25970977 |
| effect of uv-c light on the microbial and sensory quality of seasoned dried seafood. | this study investigated the effects of different doses of uv-c light at 253.7 nm (0-18 kj/m(2)) on the reduction of escherichia coli,staphylococcus aureus and bacillus cereus in contaminated seasoned dried filefish (thamnaconus modestus) and sliced squid (todarodes pacificus) surfaces and sensory quality. the counts of all three bacteria were significantly (p < 0.05) reduced by the increase of uv-c dosage.e. coli,s. aureus and b. cereus on filefish with 18 kj/m(2)of uv-c maximally reduced by 2.7 ... | 2016 | 25967394 |
| antiplatelet and antithrombotic activity of a fibrin(ogen)olytic protease from bacillus cereus strain ff01. | fibrin(ogen)olytic enzymes offer great promise for the treatment of thrombosis associated disorders. the present study describes the characterization of an extracellular fibrin(ogen)olytic serine protease (named bacethrombase) purified from the bacillus cereus strain ff01. the molecular mass of the bacethrombase was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and matrix assisted laser desorption/ionization-time-of-flight-mass spectroscopy analyses at 39.5 kda and 38,4 ... | 2015 | 25964180 |
| time dynamics of the bacillus cereus exoproteome are shaped by cellular oxidation. | at low density, bacillus cereus cells release a large variety of proteins into the extracellular medium when cultivated in ph-regulated, glucose-containing minimal medium, either in the presence or absence of oxygen. the majority of these exoproteins are putative virulence factors, including toxin-related proteins. here, b. cereus exoproteome time courses were monitored by nanolc-ms/ms under low-oxidoreduction potential (orp) anaerobiosis, high-orp anaerobiosis, and aerobiosis, with a specific f ... | 2015 | 25954265 |
| synthesis and antibacterial properties of 2,3-dideoxyglucosides of terpene alcohols and phenols. | essential oils and their oxygenated terpene constituents possess potent antimicrobial properties. in the present study, a facile synthetic route to the 2,3-dideoxy 1-o-glucosides of important phenols and terpene alcohols in excellent yields (85-96%) has been delineated. studies on their antimicrobial action against four food-borne pathogens--bacillus cereus, staphylococcus aureus, escherichia coli and yersinia enterocolitica--demonstrated that the zone of inhibition, in general, was higher for t ... | 2015 | 25952857 |
| isolation of lactic acid bacteria showing antioxidative and probiotic activities from kimchi and infant feces. | the purpose of this study was to investigate lactic acid bacteria with antioxidative and probiotic activities isolated from korean healthy infant feces and kimchi. isolates a1, a2, s1, s2, and s3 were assigned to lactobacillus sp. and isolates a3, a4, e1, e2, e3, and e4 were assigned to leuconostoc sp. on the basis of their physiological properties and 16s ribosomal dna sequence analysis. most strains were confirmed as safe bioresources through nonhemolytic activities and non-production of harmf ... | 2015 | 25951843 |
| decontamination of mesquite pod flour naturally contaminated with bacillus cereus and formation of furan by ionizing irradiation. | mesquite pod flour produced from nitrogen-fixing trees of the prosopis species has a unique aroma and flavor that is preferred by some consumers. due to the presence of wildlife, grazing domestic animals, and insects, the pods have a high potential of being contaminated with human pathogenic bacteria, such as bacillus cereus. nonthermal processing technologies are helpful to reduce the population of microorganisms in the flour because heating deteriorates the characteristic flavor. a study was c ... | 2015 | 25951390 |
| incidence, antibiotic susceptibility, and toxin profiles of bacillus cereus sensu lato isolated from korean fermented soybean products. | korean fermented soybean products, such as doenjang, kochujang, ssamjang, and cho-kochujang, can harbor foodborne pathogens such as bacillus cereus sensu lato (b. cereus sensu lato). the aim of this study was to characterize the toxin gene profiles, biochemical characteristics, and antibiotic resistance patterns of b. cereus sensu lato strains isolated from korean fermented soybean products. eighty-eight samples of korean fermented soybean products purchased from retails in seoul were tested. th ... | 2015 | 25950845 |
| assessment of microbial contamination within working environments of different types of composting plants. | the objective of the study was to determine the degree of microbiological contamination, type of microflora, bioaerosol particle size distribution, and concentration of endotoxins in dust in different types of composting plants. in addition, this study provides a list of indicator microorganisms that pose a biological threat in composting facilities, based on their prevalence within the workplace, source of isolation, and health hazards. we undertook microbiological analysis of the air, work sur ... | 2015 | 25947216 |
| effect of high hydrostatic pressure combined with moderate heat to inactivate pressure-resistant bacteria in water-boiled salted duck. | the objective of this work was to study the effect of high hydrostatic pressure combined with moderate heat to inactivate pressure-resistant bacteria in water-boiled salted duck meat (wbsdm), and to establish suitable procedures to improve the quality of wbsdm. the conditions (300 mpa/60 °c, 400 mpa/60 °c, and 500 mpa/50 °c) effectively inactivated the pressure-resistant bacteria (bacillus cereus and staphylococcus warneri) in wbsdm. although more pressure-resistant than s. warneri, the above tr ... | 2015 | 25943207 |
| bactericidal mechanism of bio-oil obtained from fast pyrolysis of pinus densiflora against two foodborne pathogens, bacillus cereus and listeria monocytogenes. | foodborne bacteria are the leading cause of food spoilage and other related diseases. in the present study, the antibacterial activity of bio-oil (bo) manufactured by fast pyrolysis of pinewood sawdust (pinus densiflora siebold and zucc.) against two disease-causing foodborne pathogens (bacillus cereus and listeria monocytogenes) was evaluated. bo at a concentration of 1000 μg/disc was highly active against both b. cereus (10.0-10.6 mm-inhibition zone) and l. monocytogenes (10.6-12.0-mm inhibiti ... | 2015 | 25928035 |
| effects of ripening duration and rosemary powder addition on salchichon modified sausage quality. | the ripening durations and ingredients for the salchichon sausages were modified to increase pork rear leg consumption by korean consumers. the salchichon, a ripened pork sausage, was produced to evaluate the efficacy of two different ripening durations with and without rosemary powder on salchichon sausage quality, and the treatments were: i) 45 days of ripening without rosemary, ii) 60 days of ripening without rosemary, iii) 45 days of ripening with 0.05% rosemary, and iv) 60 days of ripening ... | 2015 | 25924959 |
| the water cycle, a potential source of the bacterial pathogen bacillus cereus. | the behaviour of the sporulating soil-dwelling bacillus cereus sensu lato (b. cereus sl) which includes foodborne pathogenic strains has been extensively studied in relation to its various animal hosts. the aim of this environmental study was to investigate the water compartments (rain and soil water, as well as groundwater) closely linked to the primary b. cereus sl reservoir, for which available data are limited. b. cereus sl was present, primarily as spores, in all of the tested compartments ... | 2015 | 25918712 |
| chitin extraction from crab shells by bacillus bacteria. biological activities of fermented crab supernatants. | crab shells waste were fermented using six protease-producing bacillus species (bacillus subtilis a26, bacillus mojavensis a21, bacillus pumilus a1, bacillus amyloliquefaciens an6, bacillus licheniformis nh1 and bacillus cereus bg1) for the production of chitin and fermented-crab supernatants (fcss). in medium containing only crab shells, the highest demineralization dm was obtained with b. licheniformis nh1 (83±0.5%) and b. pumilus a1 (80±0.6%), while the highest deproteinization (dp) was achie ... | 2015 | 25910648 |
| melanin-like pigment synthesis by soil bacillus weihenstephanensis isolates from northeastern poland. | although melanin is known for protecting living organisms from harmful physical and chemical factors, its synthesis is rarely observed among endospore-forming bacillus cereus sensu lato. here, for the first time, we reported that psychrotolerant bacillus weihenstephanensis from northeastern poland can produce melanin-like pigment. we assessed physicochemical properties of the pigment and the mechanism of its synthesis in relation to b. weihenstephanensis genotypic and phenotypic characteristics. ... | 2015 | 25909751 |
| correction: capsules, toxins and atxa as virulence factors of emerging bacillus cereus biovar anthracis. | [this corrects the article doi: 10.1371/journal.pntd.0003455.]. | 2015 | 25902046 |
| production, optimization and characterization of polyhydroxybutyrate, a biodegradable plastic by bacillus spp. | poly-β-hydroxybutyrate (phb) is the intracellular lipid reserve accumulated by many bacteria. the most potent terrestrial bacterium bacillus cereus se-1 showed more phb accumulating cells (22.1 and 40% after 48 and 72 h) than that of the marine bacillus sp. cs-605 (5 and 33% after 48 and 72 h). both the isolates harbored phbb gene and the characteristics c=o peak was observed in the extracted phb by fourier transformed infrared spectroscopy analysis. maltose was found to be the most suitable car ... | 2015 | 25900381 |
| challenge study of the pathogen reduction capacity of the theraflex mb-plasma technology. | although most pathogen reduction systems for plasma primarily target viruses, bacterial contamination may also occur. this study aimed to investigate the bacterial reduction capacity of a methylene blue (mb) treatment process and its virus inactivation capacity in lipaemic plasma. | 2015 | 25899343 |
| design, synthesis and biological evaluation of 4-benzoyl-1-dichlorobenzoylthiosemicarbazides as potent gram-positive antibacterial agents. | twelve 4-benzoyl-1-dichlorobenzoylthiosemicarbazides have been tested as potential antibacterials. all the compounds had mics between 0.49 and 15.63 µg/ml toward micrococcus luteus, bacillus cereus, bacillus subtilis and staphylococcus epidermidis indicating, in most cases, equipotent or even more effective action than cefuroxime. in order to clarify if the observed antibacterial effects are universal, further research were undertaken to test inhibitory potency of two most potent compounds 3 and ... | 2016 | 25897586 |
| chemical constituents and biological activities of albizia myriophylla wood. | albizia myriophylla benth (leguminosae) is a medicinal plant widely used in thailand and other asian countries as a folk medicine remedy for many ailments. | 2016 | 25894212 |
| ieodoglucomide c and ieodoglycolipid, new glycolipids from a marine-derived bacterium bacillus licheniformis 09idym23. | chemical examination of the ethyl acetate extract from the fermentation broth of the marine-derived bacterium bacillus licheniformis resulted in the isolation of two new glycolipids, ieodoglucomide c (1) and ieodoglycolipid (2). the structural characterization of 1 and 2 was achieved by extensive spectroscopic evidence, including 2d nmr experiments. a combination of chemical derivatization techniques followed by nmr studies, lc-ms data analysis and a literature review was deployed for the establ ... | 2015 | 25893812 |
| forensic differentiation of bacillus cereus spores grown using different culture media using raman spectroscopy. | some microorganisms have been shown to retain a chemical signature indicative of the medium used for culturing. however, the repeatability of medium-specific chemical signatures has not been demonstrated from samples of microorganisms produced in the same batch or in different batches by the same sporulation protocol. here, the variation in raman spectra of bacterial endospores repeatedly prepared by the same procedure is compared to the variation between raman spectra of spores prepared using d ... | 2015 | 25893804 |
| bacillus cereus food poisoning: international and indian perspective. | food borne illnesses result from eating food or drinking beverages that are contaminated with chemical matter, heavy metals, parasites, fungi, viruses and bacteria. bacillus cereus is one of the food-borne disease causing bacteria. species of bacillus and related genera have long been troublesome to food producers on account of their resistant endospores. their spores may be present on various types of raw and cooked foods, and their ability to survive high cooking temperatures requires that coo ... | 2015 | 25892750 |
| unistatus - a cross-sectional study on the contamination of uniforms in the danish ambulance service. | patients are at risk of contracting infections due to the presence of disease-causing microorganisms that can be transmitted from the medical staff's uniforms to the patient. the dual purpose of this study was to examine the contamination level of the uniforms worn by ambulance staff after a shift and to test the effect of washing of the uniform with and without a detergent containing acetic peroxide. | 2015 | 25889860 |
| development of intestinal microflora and occurrence of diarrhoea in sucking foals: effects of bacillus cereus var. toyoi supplementation. | almost all foals develop transient diarrhoea within the first weeks of life. studies indicated different viral, bacterial, and parasitic causes, such as rotavirus, clostridium perfringens, escherichia coli, and cryptosporidium are discussed. but little is known about the development of intestinal microflora in foals. the present study investigated whether the supplementation with bacillus cereus var. toyoi would modify the developing intestinal microflora and consequently reduce diarrhoea in foa ... | 2015 | 25889817 |
| antimicrobial and immunomodulatory efficacy of extracellularly synthesized silver and gold nanoparticles by a novel phosphate solubilizing fungus bipolaris tetramera. | particulates of nanometers size have occupied a significant area in the field of medicinal and agricultural purposes due to their large surface-to-volume ratio and exceptional physicochemical, electronic and mechanical properties. myconanotechnology, an interface between mycology and nanotechnology is budding nowadays for nanoparticle-fabrication using fungus or its metabolites. in the present study, we have isolated and characterized a novel phosphate solubilizing fungus b. tetramera kf934408 f ... | 2015 | 25881309 |
| characterization of the spore-forming bacillus cereus sensu lato group and clostridium perfringens bacteria isolated from the australian dairy farm environment. | the bacillus cereus sensu lato group and clostridium perfringens are spore-forming bacteria often associated with food spoilage and which can cause emetic and diarrheal syndromes in humans and ruminants. this study characterised the phenotypes and genotypes of 50 bacillus cereus s. l. isolates and 26 clostridium perfringens isolates from dairy farms environments in victoria, australia. | 2015 | 25881096 |
| antimicrobial evaluation of plants used for the treatment of diarrhoea in a rural community in northern maputaland, kwazulu-natal, south africa. | zulu people living in the rural area of maputaland (kwazulu-natal, south africa) rely heavily on medicinal plants for the treatment of diarrhoea. abundant availability of medicinal plants in the study area offers low cost health care, but scientific validation is needed in order to lend credibility to the traditional use against many ailments including diarrhoeal infections. with this in mind a study was designed to test the in vitro antimicrobial efficacy of 23 plant species which are used for ... | 2015 | 25881066 |
| endophytic bacteria from pinellia ternata, a new source of purine alkaloids and bacterial manure. | pinellia ternata (thunb.) berit., a perennial herb belonging to araceae, is one of the few medicinal plants to produce purine alkaloids. it is speculated that endophytic bacteria from p. ternata may produce guanosine or inosine. however, there is no report about endophytic bacteria in p. ternata. | 2015 | 25868618 |
| secondary metabolites of seseli rigidum: chemical composition plus antioxidant, antimicrobial and cholinesterase inhibition activity. | extracts of different polarity obtained from various plant parts (root, leaf, flower and fruit) of seseli rigidum were studied by different antioxidant assays: dpph and abts radical scavenging activity, by total reducing power method as well as via total content of flavonoids and polyphenols. essential oils of all plant parts showed weak antioxidant characteristics. the inhibitory concentration range of the tested extracts, against bacteria escherichia coli, pseudomonas aeruginosa, staphylococcu ... | 2015 | 25863020 |
| characterization of cell-free extracts from fenpropathrin-degrading strain bacillus cereus zh-3 and its potential for bioremediation of pyrethroid-contaminated soils. | synthetic pyrethroid fenpropathrin has received increasing attention because of its environmental contamination and toxic effects on non-target organisms including human beings. here we report the degradation characteristics of cell-free extracts from fenpropathrin-degrading strain bacillus cereus zh-3 and its potential for pyrethroid bioremediation in soils. 50mg·l(-1) of fenpropathrin was decreased to 20.6mg·l(-1) by the enzymatic extracts (869.4mg·l(-1)) within 30min. kinetic constants km and ... | 2015 | 25862990 |
| purification and characterization of a novel plantaricin, kl-1y, from lactobacillus plantarum kl-1. | three bacteriocins from lactobacillus plantarum kl-1 were successfully purified using ammonium sulfate precipitation, cation-exchange chromatography and reverse-phase hplc. the bacteriocin peptides kl-1x, -1y and -1z had molecular masses of 3053.82, 3498.16 and 3533.16 da, respectively. all three peptides were stable at ph 2-12 and 25 °c and at high temperatures of 80 and 100 °c for 30 min and 121 °c for 15 min. however, they differed in their susceptibility to proteolytic enzymes and their inhi ... | 2015 | 25862353 |
| pulsed-field gel electrophoresis of bacillus cereus group strains. | pulsed-field gel electrophoresis (pfge) subtyping has been used extensively to characterize various bacterial species and to facilitate comparative analysis of geographically diverse populations. to this end, standardized protocols for many different genera and species have been developed, particularly through the pulsenet platform. the bacillus cereus group of bacteria includes a diverse species set, which are of particular importance in food safety as both human pathogens and spoilage organism ... | 2015 | 25862049 |
| spontaneous bacterial peritonitis (sbp) caused by bacillus cereus in an alcoholic patient: case report and review of literature. | spontaneous bacterial peritonitis (sbp) is infection of peritoneal covering of the abdomen caused by bacteria, without any known etiology. common known predisposing factors are cirrhosis of liver and old age among others. bacillus cereus is an uncommon cause of sbp and often wrongly interpreted as a contaminant. we hereby report a case of peritonitis in chronic alcoholic, elderly male patient presenting in the outpatient department. bacillus cereus is often regarded as contaminant but must be ca ... | 2015 | 25859458 |
| draft genome sequences of supercritical co2-tolerant bacteria bacillus subterraneus mitot1 and bacillus cereus mit0214. | we report draft genome sequences of bacillus subterraneus mitot1 and bacillus cereus mit0214 isolated through enrichment of samples from geologic sequestration sites in pressurized bioreactors containing a supercritical (sc) co2 headspace. their genome sequences expand the phylogenetic range of sequenced bacilli and allow characterization of molecular mechanisms of scco2 tolerance. | 2015 | 25858826 |
| antibiotic-resistant bacteria: prevalence in food and inactivation by food-compatible compounds and plant extracts. | foodborne antibiotic-resistant pathogenic bacteria such as campylobacter jejuni, bacillus cereus, clostridium perfringens, escherichia coli, salmonella enterica, staphylococcus aureus, vibrio cholerae, and vibrio parahemolyticus can adversely affect animal and human health, but a better understanding of the factors involved in their pathogenesis is needed. to help meet this need, this overview surveys and interprets much of our current knowledge of antibiotic (multidrug)-resistant bacteria in th ... | 2015 | 25856120 |
| fatty acid profiles for differentiating growth medium formulations used to culture bacillus cereus t-strain spores. | microbial biomarkers that indicate aspects of an organism's growth conditions are important targets of forensic research. in this study, we examined fatty acid composition as a signature for the types of complex nutrients in the culturing medium. bacillus cereus t-strain spores were grown in medium formulations supplemented with one of the following: peptone (meat protein), tryptone (casein protein), soy protein, and brain-heart infusion. cellular biomass was profiled with fatty acid methyl este ... | 2015 | 25854710 |
| temperature dependence of the proteome profile of the psychrotolerant pathogenic food spoiler bacillus weihenstephanensis type strain wsbc 10204. | bacillus weihenstephanensis is a subspecies of the bacillus cereus sensu lato group of spore-forming bacteria known to cause food spoilage or food poisoning. the key distinguishing phenotype of b. weihenstephanensis is its ability to grow below 7 °c or, from a food safety perspective, to grow and potentially produce toxins in a refrigerated environment. comparison of the proteome profile of b. weihenstephanensis upon its exposure to different culturing conditions can reveal clues to the mechanis ... | 2015 | 25853650 |
| organic-inorganic hybrid nanoparticles for bacterial inhibition: synthesis and characterization of doped and undoped onps with ag/au nps. | organic nanoparticles (onps) of lipoic acid and its doped derivatives onps/ag and onps/au were prepared and characterized by uv-visible, eds, and tem analysis. the antibacterial properties of the onps onps/ag and onps/au were tested against bacterial strains (staphylococcus aureus, bacillus cereus, escherichia coli and salmonella typhi). minimal inhibitory concentration (mic) and bacterial growth inhibition tests show that onps/ag are more effective in limiting bacterial growth than other nps, p ... | 2015 | 25853317 |
| protein composition of the outermost exosporium-like layer of clostridium difficile 630 spores. | clostridium difficile spores are considered the morphotype of infection, transmission and persistence of c. difficile infections. there is a lack of information on the composition of the outermost exosporium layer of c. difficile spores. using recently developed exosporium removal methods combined with ms/ms, we have established a gel-free approach to analyze the proteome of the exosporium of c. difficile spores of strain 630. a total of 184 proteins were found in the exosporium layer of c. diff ... | 2015 | 25849250 |
| biosynthesis, characterization, and antimicrobial applications of silver nanoparticles. | in the present study, the strain brevibacterium frigoritolerans dc2 was explored for the efficient and extracellular synthesis of silver nanoparticles. these biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. in addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. the dynamic l ... | 2015 | 25848272 |
| synthesis and characterization of nanosilver with antibacterial properties using pinus densiflora young cone extract. | this study describes an eco-friendly, rapid method for green synthesis of silver nanoparticles (ag nps) from an aqueous solution of silver nitrate using pinus densiflora for. multicaulis uyeki young cone extract in a single-pot process. color changes, ultraviolet-visible spectra (444.5 nm), x-ray diffraction peaks (2θ=39.68, 46.92, 68.12, and 79.10), and fourier transform infrared spectroscopy (ft-ir) confirmed the presence of ag nps and phytochemicals. transmission electron microscopy showed th ... | 2015 | 25846578 |
| biodegradation of carbofuran in soils within nzoia river basin, kenya. | carbofuran (2,3-dihydro-2,2-dimethylbenzofuran-7-yl methylcarbamate) has been used within the nzoia river basin (nrb), especially in bunyala rice irrigation schemes, in kenya for the control of pests. in this study, the capacity of native bacteria to degrade carbofuran in soils from nrb was investigated. a gram positive, rod-shaped bacteria capable of degrading carbofuran was isolated through liquid cultures with carbofuran as the only carbon and nitrogen source. the isolate degraded 98% of 100- ... | 2015 | 25844859 |
| a rapid and miniaturized method for the selection of microbial phenol degraders using colourimetric microtitration. | a high-throughput method is described, consisting of a colourimetric microtitration for screening phenol-degrading microorganisms, using a mixture of 4-aminoantipyrine and potassium ferricyanide as the colour indicator. this contemporary study summarizes a new method to determine phenol-degrading bacteria isolated from different areas. the method was used for testing a total of 72 bacteria collected from the natural environment and five known strains obtained from diagnostic and research laborat ... | 2015 | 25842173 |
| characterization of films based on chitosan lactate and its blends with oxidized starch and gelatin. | minimal inhibitory concentration (mic) of chitosan lactate (chl) was tested against bacteria and phytopathogenic fungi. then, the structural, physicochemical and antimicrobial properties of films based on chl, oxidized potato starch (ops), and gelatin (gel) were investigated. with the exception of rhizopus nigricans, chl was effective against the target organisms. gram-positive bacteria (staphylococcus aureus and bacillus cereus) were more sensitive to chl than gram-negative bacteria (pectobacte ... | 2015 | 25841370 |
| microbiological diversity and prevalence of spoilage and pathogenic bacteria in commercial fermented alcoholic beverages (beer, fruit wine, refined rice wine, and yakju). | the present study examined 469 commercially available fermented alcoholic beverages (fabs), including beer (draft, microbrewed, and pasteurized), fruit wine (grape and others), refined rice wine, and yakju (raw and pasteurized). samples were screened for escherichia coli and eight foodborne pathogens (bacillus cereus, campylobacter jejuni, clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella spp., staphylococcus aureus, and yersinia enterocolitica), and the aerob ... | 2015 | 25836410 |
| expert elicitation as a means to attribute 28 enteric pathogens to foodborne, waterborne, animal contact, and person-to-person transmission routes in canada. | enteric illness contributes to a significant burden of illness in canada and globally. understanding its sources is a critical step in identifying and preventing health risks. expert elicitation is a powerful tool, used previously, to obtain information about enteric illness source attribution where information is difficult or expensive to obtain. thirty-one experts estimated transmission of 28 pathogens via major transmission routes (foodborne, waterborne, animal contact, person-to-person, and ... | 2015 | 25835810 |
| capsules, toxins and atxa as virulence factors of emerging bacillus cereus biovar anthracis. | emerging b. cereus strains that cause anthrax-like disease have been isolated in cameroon (ca strain) and côte d'ivoire (ci strain). these strains are unusual, because their genomic characterisation shows that they belong to the b. cereus species, although they harbour two plasmids, pbcxo1 and pbcxo2, that are highly similar to the pxo1 and pxo2 plasmids of b. anthracis that encode the toxins and the polyglutamate capsule respectively. the virulence factors implicated in the pathogenicity of the ... | 2015 | 25830379 |
| antimicrobial activities of active component isolated from lawsonia inermis leaves and structure-activity relationships of its analogues against food-borne bacteria. | the antimicrobial activities of lawsonia inermis leaf extract and 2-hydroxy-1,4-naphthoquinone analogues against food-borne bacteria. the antimicrobial activities of five fractions derived from the methanol extract of lawsonia inermis leaves were evaluated against 7 food-borne bacteria. 2-hydroxy-1,4-naphthoquinone was isolated by chromatographic analyses. 2-hydroxy-1,4-naphthoquinone showed the strong activities against bacillus cereus, listeria monocytogenes, salmonella enterica, shigella sonn ... | 2015 | 25829631 |
| alternative modes of biofilm formation by plant-associated bacillus cereus. | the ability to form multicellular communities known as biofilms is a widespread adaptive behavior of bacteria. members of the bacillus group of bacteria have been found to form biofilms on plant roots, where they protect against pathogens and promote growth. in the case of the model bacterium bacillus subtilis the genetic pathway controlling biofilm formation and the production of an extracellular matrix is relatively well understood. however, it is unclear whether other members of this genus ut ... | 2015 | 25828975 |
| gcogsa-ba: a global core genome snp analysis for bacillus anthracis. | as an issue of biosecurity, it is important to identify the origin of a suspected sample to distinguish whether it originated from the release of a bioterrorism agent or from environmental contamination with a virulent agent. here we have developed an analytical pipeline that can infer the phylogenetic position of bacillus cereus group species, including b. anthracis, from next-generation sequencing reads without extensive genomics skills. gcogsa-ba can also detect the existence of anthrax plasm ... | 2016 | 25812430 |
| structure, activity and stereoselectivity of nadph-dependent oxidoreductases catalysing the s-selective reduction of the imine substrate 2-methylpyrroline. | oxidoreductases from streptomyces sp. gf3546 [3546-ired], bacillus cereus bag3x2 (bcired) and nocardiopsis halophila (nhired) each reduce prochiral 2-methylpyrroline (2mpn) to (s)-2-methylpyrrolidine with >95 % ee and also a number of other imine substrates with good selectivity. structures of bcired and nhired have helped to identify conserved active site residues within this subgroup of imine reductases that have s selectivity towards 2mpn, including a tyrosine residue that has a possible role ... | 2015 | 25809902 |
| antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia). | antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ... | 2015 | 25803299 |
| maldi-tof ms and cd spectral analysis for identification and structure prediction of a purified, novel, organic solvent stable, fibrinolytic metalloprotease from bacillus cereus b80. | the ability to predict protein function from structure is becoming increasingly important; hence, elucidation and determination of protein structure become the major steps in proteomics. the present study was undertaken for identification of metalloprotease produced by bacillus cereus b80 and recognition of characteristics that can be industrially exploited. the enzyme was purified in three steps combining precipitation and chromatographic methods resulting in 33.5% recovery with 13.1-fold purif ... | 2015 | 25802851 |
| bacillus cereus from the environment is genetically related to the highly pathogenic b. cereus in zambia. | to follow-up anthrax in zambia since the outbreak in 2011, we have collected samples from the environment and the carcasses of anthrax-suspected animals, and have tried to isolate bacillus anthracis. in the process of identification of b. anthracis, we collected two isolates, of which colonies were similar to b. anthracis; however, from the results of identification using the molecular-based methods, two isolates were genetically related to the highly pathogenic b. cereus, of which clinical mani ... | 2015 | 25797134 |
| the effect of selected factors on the survival of bacillus cereus in the human gastrointestinal tract. | bacillus cereus is a gram-positive bacterium widely distributed in soil and vegetation. this bacterial species can also contaminate raw or processed foods. pathogenic b. cereus strains can cause a range of infections in humans, as well as food poisoning of an emetic (intoxication) or diarrheal type (toxico-infection). toxico-infections are due to the action of the hbl toxin, nhe toxin, and cytotoxin k produced by the microorganism in the gastrointestinal tract. this occurs once the spores or veg ... | 2015 | 25794697 |
| determination and quantification of the emetic toxin cereulide from bacillus cereus in pasta, rice and cream with liquid chromatography-tandem mass spectrometry. | a rapid and sensitive method has been developed for determination and quantification of cereulide in cream, rice and pasta. samples are homogenised after addition of amylase to cooked rice and pasta, and cereulide is extracted with methanol. after the removal of water with methyl-tert butyl ether/hexane and evaporation until dryness, no further purification was required before analysis with liquid chromatography-tandem mass spectrometry (lc-ms/ms). recently, both cereulide and (13)c6-cereulide h ... | 2015 | 25794364 |
| various enterotoxin and other virulence factor genes widespread among bacillus cereus and bacillus thuringiensis strains. | many strains of bacillus cereus cause gastrointestinal diseases, and the closely related insect pathogen bacillus thuringiensis has also been involved in outbreaks of diarrhea. the diarrheal diseases are attributed to enterotoxins. sixteen reference strains of b. cereus and nine commercial and 12 reference strains of b. thuringiensis were screened by pcr for the presence of 10 enterotoxigenic genes (hbla, hblc, hbld, nhea, nheb, nhec, cytk, bcet, entfm, and ents), one emetogenic gene (ces), seve ... | 2015 | 25791850 |
| effect of thymol in heating and recovery media on the isothermal and non-isothermal heat resistance of bacillus spores. | members of the genus bacillus include important food-borne pathogen and spoilage microorganisms for food industry. essential oils are natural products extracted from herbs and spices, which can be used as natural preservatives in many foods because of their antibacterial, antifungal, antioxidant and anti-carcinogenic properties. the aim of this research was to explore the effect of the addition of different concentrations of thymol to the heating and recovery media on the thermal resistance of s ... | 2015 | 25790989 |
| grafted α-hydroxyphosphonic acids onto polymeric supports: preparation, characterization, and antimicrobial effect. | the paper deals with the preparation and characterization of compounds with antimicrobial activity: α-hydroxyphosphonic acids grafted onto styrene-12%-(15%)-divinylbenzene copolymer. these products proved to have antimicrobial effect against two species of gram-negative bacteria (escherichia coli and pseudomonas aeruginosa) and two species of gram-positive bacteria (staphylococcus aureus and bacillus cereus) and a species of yeast (candida albicans). tests showed the reduction of bacterial load ... | 2015 | 25789417 |
| a novel multiplex pcr discriminates bacillus anthracis and its genetically related strains from other bacillus cereus group species. | anthrax is an important zoonotic disease worldwide that is caused by bacillus anthracis, a spore-forming pathogenic bacterium. a rapid and sensitive method to detect b. anthracis is important for anthrax risk management and control in animal cases to address public health issues. however, it has recently become difficult to identify b. anthracis by using previously reported molecular-based methods because of the emergence of b. cereus, which causes severe extra-intestinal infection, as well as t ... | 2015 | 25774512 |
| antimicrobial and antiquorum-sensing studies. part 3: synthesis and biological evaluation of new series of [1,3,4]thiadiazoles and fused [1,3,4]thiadiazoles. | new series of [1,3,4]thiadiazoles and fused [1,3,4]thiadiazoles were synthesized. the newly synthesized compounds were screened for their antibacterial activity against staphylococcus aureus, bacillus cereus, and escherichia coli. compounds 3b and 10a displayed the highest activity against e. coli with mic value of 78.125 μg/ml. in addition, compound 10a exhibited the highest activity against b. cereus with mic value of 156.25 μg/ml. the antifungal activity of these compounds was also tested aga ... | 2015 | 25773477 |
| the development of a green approach for the biosynthesis of silver and gold nanoparticles by using panax ginseng root extract, and their biological applications. | the biosynthesis of nanoparticles has received attention because of the development of economic and environmentally friendly technology for the synthesis of nanoparticles. the study develops a convenient method for the green synthesis of silver and gold nanoparticles by utilizing fresh root extract of the four-year old panax ginseng plant, and evaluated the antimicrobial applications of silver nanoparticles against pathogenic microorganisms. p. ginseng is a well-known herbal medicinal plant, and ... | 2016 | 25771716 |
| genetic characterization, nickel tolerance, biosorption, kinetics, and uptake mechanism of a bacterium isolated from electroplating industrial effluent. | electroplating industries in madurai city produce approximately 49,000 l of wastewater and 1200 l of sludge every day revealing 687-5569 ppm of nickel (ni) with other contaminants. seventeen ni-tolerant bacterial strains were isolated from nutrient-enriched effluents. among them one hyper ni accumulating strain was scored and identified as bacillus cereus vp17 on the basis of morphology, biochemical tests, 16s rdna gene sequencing, and phylogenetic analysis. equilibrium data of ni(ii) ions using ... | 2015 | 25768053 |
| plant compounds enhance the assay sensitivity for detection of active bacillus cereus toxin. | bacillus cereus is an important food pathogen, producing emetic and diarrheal syndromes, the latter mediated by enterotoxins. the ability to sensitively trace and identify this active toxin is important for food safety. this study evaluated a nonradioactive, sensitive, in vitro cell-based assay, based on b. cereus toxin inhibition of green fluorescent protein (gfp) synthesis in transduced monkey kidney vero cells, combined with plant extracts or plant compounds that reduce viable count of b. cer ... | 2015 | 25767986 |
| lactic acid bacteria: promising supplements for enhancing the biological activities of kombucha. | kombucha is sweetened black tea that is fermented by a symbiosis of bacteria and yeast embedded within a cellulose membrane. it is considered a health drink in many countries because it is a rich source of vitamins and may have other health benefits. it has previously been reported that adding lactic acid bacteria (lactobacillus) strains to kombucha can enhance its biological functions, but in that study only lactic acid bacteria isolated from kefir grains were tested. there are many other natur ... | 2015 | 25763303 |
| estimation of the growth kinetic parameters of bacillus cereus spores as affected by pulsed light treatment. | quantitative microbial risk assessment requires the knowledge of the effect of food preservation technologies on the growth parameters of the survivors of the treatment. this is of special interest in the case of the new non-thermal technologies that are being investigated for minimal processing of foods. this is a study on the effect of pulsed light technology (pl) on the lag phase of bacillus cereus spores surviving the treatment and the maximum growth rate (μmax) of the survivors after germin ... | 2015 | 25755081 |
| bacillus halodurans strain c125 encodes and synthesizes enzymes from both known pathways to form dump directly from cytosine deoxyribonucleotides. | analysis of the genome of bacillus halodurans strain c125 indicated that two pathways leading from a cytosine deoxyribonucleotide to dump, used for dtmp synthesis, were encoded by the genome of the bacterium. the genes that were responsible, the comeb gene and the dcdb gene, encoding dcmp deaminase and the bifunctional dctp deaminase:dutpase (dcd:dut), respectively, were both shown to be expressed in b. halodurans, and both genes were subject to repression by the nucleosides thymidine and deoxyc ... | 2015 | 25746996 |
| characterization and in vitro biological activities of thai traditional fermented shrimp pastes. | in this work, chemical and biological characteristics of two types of thai fermented shrimp paste, kapi ta dam and kapi ta deang, at different fermentation periods and their raw materials were investigated. kapi had low water activity and high proteins with high glutamic acid and lysine. both kapis, which had different sources, showed similar characteristics. the number of lactic acid bacteria in the products increased during the early stages of fermentation. free α-amino acid contents in the pr ... | 2015 | 25745266 |
| incidence and enterotoxigenic profile of bacillus cereus in meat and meat products of uttarakhand, india. | the present investigation was undertaken to study the incidence and enterotoxigenicity of bacillus cereus in raw meat and meat products. b. cereus was isolated from 29 (30.9 %) of the 94 samples analyzed. recorded incidences of b. cereus from raw meat and meat products samples were 27.8 and 35 %, respectively. a high level of organism was found in cooked-meat (35 %) than raw meat samples (27.78 %) from 40 cooked-meat products and 54 raw meat samples analyzed. screening of isolates by multiplex p ... | 2015 | 25745259 |
| changes in physicochemical characteristics of ozone-treated raw white rice. | ozone dose from 0.1 to 0.4 ppm has been proven to be effective in lowering bacillus cereus count in uncooked and cooked rice. however, it induces physicochemical changes in raw white rice. physicochemical tests were done to see the effect of ozone treatment towards moisture content, ph, color, hardness of uncooked rice, adhesiveness and hardness of cooked rice, cooking quality and total solids. results have shown that moisture content, adhesiveness and hardness of cooked rice and uncooked rice h ... | 2015 | 25745221 |
| chemoenzymatic synthesis of thiazolyl peptide natural products featuring an enzyme-catalyzed formal [4 + 2] cycloaddition. | thiocillins from bacillus cereus atcc 14579 are members of the well-known thiazolyl peptide class of natural product antibiotics, the biosynthesis of which has recently been shown to proceed via post-translational modification of ribosomally encoded precursor peptides. it has long been hypothesized that the final step of thiazolyl peptide biosynthesis involves a formal [4 + 2] cycloaddition between two dehydroalanines, a unique transformation that had eluded enzymatic characterization. here we d ... | 2015 | 25742119 |
| characterization of deep sea fish gut bacteria with antagonistic potential, from centroscyllium fabricii (deep sea shark). | the bacterial isolates from centroscyllium fabricii (deep sea shark) gut were screened for antagonistic activity by cross-streak method and disc diffusion assay. this study focuses on strain btss-3, which showed antimicrobial activity against pathogenic bacteria including salmonella typhimurium, proteus vulgaris, clostridium perfringens, staphylococcus aureus, bacillus cereus, bacillus circulans, bacillus macerans and bacillus pumilus. btss3 was subjected to phenotypic characterization using bio ... | 2015 | 25740801 |
| adaptive responses of bacillus cereus atcc14579 cells upon exposure to acid conditions involve atpase activity to maintain their internal ph. | this study examined the involvement of atpase activity in the acid tolerance response (atr) of bacillus cereus atcc14579 strain. in the current work, b. cereus cells were grown in anaerobic chemostat culture at external ph (phe ) 7.0 or 5.5 and at a growth rate of 0.2 h(-1) . population reduction and internal ph (phi ) after acid shock at ph 4.0 was examined either with or without atpase inhibitor n,n'-dicyclohexylcarbodiimide (dccd) and ionophores valinomycin and nigericin. population reduction ... | 2015 | 25740257 |
| differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells. | silver nanoparticles (agnps) possess unique physical, chemical, and biological properties. agnps have been increasingly used as anticancer, antiangiogenic, and antibacterial agents for the treatment of bacterial infections in open wounds as well as in ointments, bandages, and wound dressings. the present study aimed to investigate the effects of two different sizes of agnps (10 nm and 20 nm) in male somatic leydig (tm3) and sertoli (tm4) cells and spermatogonial stem cells (sscs). | 2015 | 25733828 |
| the effect of polyphenols isolated from cynanchi wilfordii radix with anti-inflammatory, antioxidant, and anti-bacterial activity. | recently, cynanchi wilfordii radix has gained wide use in asian countries as a functional food effective for relieving fatigue, osteoporosis, and constipation, particularly in menopausal disorders. however, its anti-inflammatory and anti-microbial activities have not been explored in detail to date. the anti-inflammatory, antioxidant, and anti-bacterial properties of the cynanchi wilfordii radix extracts obtained with water, methanol, ethanol, and acetone were compared. all 4 polyphenol-containi ... | 2015 | 25729277 |
| influence of food matrix on outgrowth heterogeneity of heat damaged bacillus cereus spores. | spoilage of heat treated foods can be caused by the presence of surviving spore-formers. it is virtually impossible to prevent contamination at the primary production level as spores are ubiquitous present in the environment and can contaminate raw products. as a result spore inactivation treatments are widely used by food producing industries to reduce the microbial spore loads. however consumers prefer mildly processed products that have less impact on its quality and this trend steers industr ... | 2015 | 25727186 |
| distinguishing the dominant species of pathogen in maxillary sinusitis by sequencing dna dataset analysis. | this study determined the microbial composition in maxillary sinusitis and determined the predominant fungal and bacterial species. samples were obtained from 11 patients with fungal sinusitis and 2 healthy patients. the 13 samples were sequenced using an illumina hi-seq 2000, and mapping to the human, bacterial, and the fungal genomes were based on several steps. bioinformatics and statistical analyses were then performed for bacterial and fungal expressions in the samples. all samples were cul ... | 2015 | 25724392 |
| [specific bacterial diversity in bats of different food guilds in southern sierra oaxaca, mexico]. | specific bacterial diversity in bats of different food guilds in southern sierra oaxaca, mexico. bats have different ecologic roles in variable ecosystems that have been already described. they have been linked to several zoonoses, however little is known about the relationship between bat microbiota and their diet, and studies on the bacterial ecology of the microbiota in bats are limited. to contribute with the description of this important interaction between microbiota and host, the aim of t ... | 2014 | 25720196 |
| spore prevalence and toxigenicity of bacillus cereus and bacillus thuringiensis isolates from u.s. retail spices. | recent incidents of foodborne illness associated with spices as the vehicle of transmission prompted this examination of u.s. retail spices with regard to bacillus cereus. this study focused on the levels of aerobic-mesophilic spore-forming bacteria and b cereus spores associated with 247 retail spices purchased from five states in the united states. samples contained a wide range of aerobic-mesophilic bacterial spore counts (< 200 to 8.3 × 10(7) cfu/g), with 19.1% of samples at levels above 10( ... | 2015 | 25719886 |
| thiopeptide antibiotics stimulate biofilm formation in bacillus subtilis. | bacteria have evolved the ability to produce a wide range of structurally complex natural products historically called "secondary" metabolites. although some of these compounds have been identified as bacterial communication cues, more frequently natural products are scrutinized for antibiotic activities that are relevant to human health. however, there has been little regard for how these compounds might otherwise impact the physiology of neighboring microbes present in complex communities. bac ... | 2015 | 25713360 |
| catalytic and thermodynamic properties of glycosylated bacillus cereus cyclodextrin glycosyltransferase. | cyclodextrin glycosyltransferase (cgtase) was covalently coupled to five oxidized polysaccharides differing in structure and chemical nature. the conjugates were evaluated for the retained activity, kinetic and thermodynamic stability. the conjugated cgtase with oxidized dextran (mw 47000) had the highest retained specific activity (70.05%) and the highest half-life (t1/2) at 80°c. compared to the native enzyme, the conjugated preparation exhibited higher optimum temperature, lower activation en ... | 2015 | 25709024 |
| mechanical and antibacterial properties of novel high performance chitosan/nanocomposite films. | zinc oxide (zno) nanoparticle was successfully synthesized using hydrothermal method as well as, silver (ag) nanoparticle was direct prepared during chitosan nanocomposites preparation. chitosan films were investigated by recognized the effect of kinds of acids (formic or acetic acid). correspondingly, using of concentrated naoh was checking for facilitating de-casting of the films. utilization of formic acid arisen higher quality films than those films produced using acetic acid as solvent. opt ... | 2015 | 25709016 |
| antibacterial, antifungal and in vitro antileukaemia activity of metal complexes with thiosemicarbazones. | 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone 4-ethyl-thiosemicarbazone (hl) and its copper(ii), vanadium(v) and nickel(ii) complexes: [cu(l)(cl)]·c₂h₅oh·(1), [cu(l)₂]·h₂o (2), [cu(l)(br)]·h₂o·ch₃oh (3), [cu(l)(no₃)]·2c₂h₅oh (4), [vo₂(l)]·2h₂o (5), [ni(l)₂]·h₂o (6), were synthesized and characterized. the ligand has been characterized by elemental analyses, ir, (1) h nmr and (13) c nmr spectroscopy. the tridentate nature of the ligand is evident from the ir spectra. the copper(ii), vanadium(v) and ni ... | 2015 | 25708540 |
| biogenic silver and gold nanoparticles synthesized using red ginseng root extract, and their applications. | in the present study, we report a green methodology for the synthesis of silver and gold nanoparticles, using the root extract of the herbal medicinal plant korean red ginseng. the silver and gold nanoparticles were synthesized within 1 h and 10 min respectively. the nanoparticles generated were not aggregated, and remained stable for a long time, which suggests the nature of nanoparticles. the phytochemicals and ginsenosides present in the root extract assist in reducing and stabilizing the syn ... | 2016 | 25706249 |
| improved in vitro assay for determining the mucin adherence of bacteria sensitive to triton x-100 treatment. | mucin-associated microbiota are in relatively close contact with the intestinal epithelium and may thus have a more pronounced effect on host health. we have previously developed a simple mucin agar assay to simulate initial mucus colonization by intestinal microbial communities. adherence of microbiota was estimated using flow cytometry after detachment with triton x-100. in this study, the effect of this detergent on the cultivability of both virulent and commensal strains was investigated. mu ... | 2015 | 25702162 |
| comparative analysis of biofilm formation by bacillus cereus reference strains and undomesticated food isolates and the effect of free iron. | biofilm formation of bacillus cereus reference strains atcc 14579 and atcc 10987 and 21 undomesticated food isolates was studied on polystyrene and stainless steel as contact surfaces. for all strains, the biofilm forming capacity was significantly enhanced when in contact with stainless steel (ss) as a surface as compared to polystyrene (ps). for a selection of strains, the total cfu and spore counts in biofilms were determined and showed a good correlation between cfu counts and total biomass ... | 2015 | 25700364 |
| polydiacetylene nanovesicles as carriers of natural phenylpropanoids for creating antimicrobial food-contact surfaces. | the ultimate goal of this study was developing antimicrobial food-contact materials based on natural phenolic compounds using nanotechnological approaches. among the methyl-β-cyclodextrin-encapsulated phenolics tested, curcumin showed by far the highest activity toward escherichia coli with a minimum inhibitory concentration of 0.4 mm. curcumin was enclosed in liposome-type polydiacetylene/phosholipid nanovesicles supplemented with n-hydroxysuccinimide and glucose. the fluorescence spectrum of t ... | 2015 | 25697369 |
| the length of a lantibiotic hinge region has profound influence on antimicrobial activity and host specificity. | lantibiotics are ribosomally synthesized (methyl)lanthionine containing peptides which can efficiently inhibit the growth of gram-positive bacteria. as lantibiotics kill bacteria efficiently and resistance to them is difficult to be obtained, they have the potential to be used in many applications, e.g., in pharmaceutical industry or food industry. nisin can inhibit the growth of gram-positive bacteria by binding to lipid ii and by making pores in their membrane. the c-terminal part of nisin is ... | 2015 | 25688235 |
| structure analysis of bacillus cereus mepr-like transcription regulator, bc0657, in complex with pseudo-ligand molecules. | the marr family of transcriptional regulatory proteins in bacteria and archaea respond to environmental changes and regulate transcriptional processes by ligand binding or cysteine oxidation. mepr belongs to the marr family, and its mutations are associated with the development of multidrug resistances, causing a growing health problem. therefore, it has been of great interest to locate the ligand binding site of mepr and reveal the ligand-mediated transcriptional regulation mechanism. here, we ... | 2015 | 25684184 |
| microbial growth under supercritical co2. | growth of microorganisms in environments containing co2 above its critical point is unexpected due to a combination of deleterious effects, including cytoplasmic acidification and membrane destabilization. thus, supercritical co2 (scco2) is generally regarded as a sterilizing agent. we report isolation of bacteria from three sites targeted for geologic carbon dioxide sequestration (gcs) that are capable of growth in pressurized bioreactors containing scco2. analysis of 16s rrna genes from scco2 ... | 2015 | 25681188 |
| isolation and characterization of glacier vmy22, a novel lytic cold-active bacteriophage of bacillus cereus. | as a unique ecological system with low temperature and low nutrient levels, glaciers are considered a "living fossil" for the research of evolution. in this work, a lytic cold-active bacteriophage designated vmy22 against bacillus cereus myb41-22 was isolated from mingyong glacier in china, and its characteristics were studied. electron microscopy revealed that vmy22 has an icosahedral head (59.2 nm in length, 31.9 nm in width) and a tail (43.2 nm in length). bacteriophage vmy22 was classified a ... | 2015 | 25680445 |
| pyrosequencing analysis of microbial community and food-borne bacteria on restaurant cutting boards collected in seri kembangan, malaysia, and their correlation with grades of food premises. | this study adopts the pyrosequencing technique to identify bacteria present on 26 kitchen cutting boards collected from different grades of food premises around seri kembangan, a city in malaysia. pyrosequencing generated 452,401 of total reads of otus with an average of 1.4×10(7) bacterial cells/cm(2). proteobacteria, firmicutes and bacteroides were identified as the most abundant phyla in the samples. taxonomic richness was generally high with >1000 operational taxonomic units (otus) observed ... | 2015 | 25679309 |
| developing novel bacterial based bioformulation having pgpr properties for enhanced production of agricultural crops. | plant growth promoting rhizobacteria (pgpr) are beneficial rhizobacteria which enhance plant growth as well as the productivity by a variety of mechanisms pgpr were isolated from the rhizosphere region of som plants (machilus bombycina king) maintained at the central muga eri research and training institute, lahdoigarh, jorhat. a bacterial based bioformulation was prepared and sprayed over the experimental crops including tomato (solanum lycopersicum), cauliflower (brassica oleracea var botrytis ... | 2015 | 25675713 |