Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| uranium complexes formed at hematite surfaces colonized by sulfate-reducing bacteria. | modeling uranium (u) transport in subsurface environments requires a thorough knowledge of mechanisms likely to restrict its mobility, such as surface complexation, precipitation, and colloid formation. in closed systems, sulfate-reducing bacteria (srb) such as desulfovibrio spp. demonstrably affect u immobilization by enzymatic reduction of u(vi) species (primarily the uranyl ion, uo2(2+), and its complexes) to u(iv). however, our understanding of such interactions under chronic u(vi) exposure ... | 2004 | 15224730 |
| structural stability of adenylate kinase from the sulfate-reducing bacteria desulfovibrio gigas. | a novel adenylate kinase (ak) has recently been purified from desulfovibrio gigas and characterized as a co(2+)/zn(2+)-containing enzyme: this is an unusual characteristic for aks from gram-negative bacteria, in which these enzymes are normally devoid of metals. here, we studied the conformational stability of holo- and apo-ak as a function of temperature by differential scanning calorimetry (dsc), circular dichroism (cd), and intrinsic fluorescence spectroscopy. the thermal unfolding of ak is a ... | 2004 | 15223146 |
| reduction of cr(vi) by "palladized" biomass of desulfovibrio desulfuricans atcc 29577. | a novel catalytic activity of palladium [pd(0)]-coated cells of desulfovibrio desulfuricans atcc 29577 ["bio-pd(0)"] is demonstrated. reduction of 700 microm cr(vi) occurred within 24 h using formate (25 mm) or hydrogen (1 atm) as the electron donor, under conditions whereby cells lacking bound pd(0), or palladium metal manufactured via chemical reduction of soluble pd(ii), did not reduce cr(vi). the biomass-bound pd(0) also functioned in the continuous removal of 400 microm cr(vi) from a 1 mm s ... | 2004 | 15211494 |
| dynamics of lead immobilization in sulfate reducing biofilms. | we have evaluated the effects of selected minerals present in subsoil environment on the efficiency of lead removal from contaminated groundwaters using biofilms composed of sulfate-reducing microorganisms, and examined the stability of metal deposits after the biofilms had been temporarily exposed to the air. to quantify the studied effects, lead was immobilized in biofilms of desulfovibrio desulfuricans grown anaerobically in two flat-plate flow reactors, one filled with hematite and the other ... | 2004 | 15207603 |
| overexpression, purification and immunodetection of dsrd from desulfovibrio vulgaris hildenborough. | dissimilatory sulfite reductase (dsrab) of the sulfate-reducing bacterium desulfovibrio vulgaris hildenborough is an alpha2beta2 tetramer of 180 kda, encoded by the dsr operon. in addition to the dsra and dsrb genes, this operon contains a gene (dsrd) encoding a protein of only 78 amino acids. although, the function of dsrd is currently unknown, the presence of a dsrd gene has been demonstrated in a variety of sulfate-reducing bacteria and archaea. dsrd was expressed in escherichia coli at a ver ... | 2000 | 15188893 |
| characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time pcr and effects of antibiotic treatment on the fecal microbiota. | fecal bacteria were studied in healthy elderly volunteers (age, 63 to 90 years; n = 35) living in the local community, elderly hospitalized patients (age, 66 to 103; n = 38), and elderly hospitalized patients receiving antibiotic treatment (age, 65 to 100; n = 21). group- and species-specific primer sets targeting 16s rrna genes were used to quantitate intestinal bacteria by using dna extracted from feces and real-time pcr. the principal difference between healthy elderly volunteers and both pat ... | 2004 | 15184159 |
| ftir spectroelectrochemical study of the activation and inactivation processes of [nife] hydrogenases: effects of solvent isotope replacement and site-directed mutagenesis. | the kinetics of the activation and anaerobic inactivation processes of desulfovibrio gigas hydrogenase have been measured in d(2)o by ftir spectroelectrochemistry. a primary kinetic solvent isotope effect was observed for the inactivation process but not for the activation step. the kinetics of these processes have been also measured after replacement of a glutamic residue placed near the active site of an analogous [nife] hydrogenase from desulfovibrio fructosovorans. its replacement by a gluta ... | 2004 | 15175937 |
| susceptibility of desulfovibrio desulfuricans intestinal strains to sulfasalazine and its biotransformation products. | desulfovibrio desulfuricans intestinal bacteria may contribute to toxic hydrogen sulfide production in the human gut. our objective was to examine whether the d. desulfuricans strains isolated from the human body are susceptible to sulfasalazine (sas) and the products of its biotransformation, i.e. 5-aminosalicylic acid (5-asa) and sulfapyridine (sp), in order to determine the relationship between the strains' susceptibility to sas and their ability to reduce the azo bond within this drug. | 2004 | 15173665 |
| 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head with a bifunctional enzyme that controls glycolysis. | fru-2,6-p2 (fructose 2,6-bisphosphate) is a signal molecule that controls glycolysis. since its discovery more than 20 years ago, inroads have been made towards the understanding of the structure-function relationships in pfk-2 (6-phosphofructo-2-kinase)/fbpase-2 (fructose-2,6-bisphosphatase), the homodimeric bifunctional enzyme that catalyses the synthesis and degradation of fru-2,6-p2. the fbpase-2 domain of the enzyme subunit bears sequence, mechanistic and structural similarity to the histid ... | 2004 | 15170386 |
| relativistic dft calculation of the reaction cycle intermediates of [nife] hydrogenase: a contribution to understanding the enzymatic mechanism. | structures and spectroscopic observables of the paramagnetic intermediates of the enzymatic reaction cycle of the metalloenzyme [nife] hydrogenase were calculated using relativistic density functional theory (dft) within the zero-order regular approximation (zora). by comparing experimental and calculated magnetic resonance parameters (g- and hyperfine tensors) for the states ni-a, ni-b, ni-c, ni-l, and ni-co the details of the atomic composition of these paramagnetic intermediates could be eluc ... | 2004 | 15134933 |
| antagonists mo and cu in a heterometallic cluster present on a novel protein (orange protein) isolated from desulfovibrio gigas. | an orange-coloured protein (orp) isolated from desulfovibrio gigas, a sulphate reducer, has been previously shown by extended x-ray absorption fine structure (exafs) to contain a novel mixed-metal sulphide cluster of the type [s(2)mos(2)cus(2)mos(2)] [j. am. chem. soc. 122 (2000) 8321]. we report here the purification and the biochemical/spectroscopic characterisation of this novel protein. orp is a soluble monomeric protein (11.8 kda). the cluster is non-covalently bound to the polypeptide chai ... | 2004 | 15134929 |
| displacement of iron by zinc at the diiron site of desulfovibrio vulgaris rubrerythrin: x-ray crystal structure and anomalous scattering analysis. | x-ray crystal structures of recombinant desulfovibrio (d.) vulgaris rubrerythrin (rbr) have shown a diiron site, whereas the crystal structure of rbr "as-isolated" from d. vulgaris was reported to contain a mixed zn,fe binuclear site. to investigate the possibility that zinc had displaced iron during isolation or crystallization of the "as-isolated" d. vulgaris rbr, the x-ray crystal structure of recombinant d. vulgaris all-iron rbr that had been incubated with excess zinc sulfate prior to cryst ... | 2004 | 15134924 |
| automated purification and suspension array detection of 16s rrna from soil and sediment extracts by using tunable surface microparticles. | autonomous, field-deployable molecular detection systems require seamless integration of complex biochemical solutions and physical or mechanical processing steps. in an attempt to simplify the fluidic requirements for integrated biodetection systems, we used tunable surface microparticles both as an rrna affinity purification resin in a renewable microcolumn sample preparation system and as the sensor surface in a flow cytometer detector. the tunable surface detection limits in both low- and hi ... | 2004 | 15128511 |
| carbon monoxide and oxidative stress in desulfovibrio desulfuricans b-1388. | it has been shown that carbon monoxide (co) in low concentration may be an active biochemical and physiological regulator of cell function. the bases of co toxicity and cell protection are not clearly understood. to provide insights into these mechanisms, we measured superoxide production by d. desulfuricans b-1388 incubated anaerobically in postgate medium with or without 5% co. d. desulfuricans b-1388 growing with co in the gas phase produced more superoxide radicals then control cells growing ... | 2004 | 15122650 |
| interaction between uranium and the cytochrome c3 of desulfovibrio desulfuricans strain g20. | cytochrome c(3) of desulfovibrio desulfuricans strain g20 is an electron carrier for uranium (vi) reduction. when d. desulfuricans g20 was grown in medium containing a non-lethal concentration of uranyl acetate (1 mm), the rate at which the cells reduced u(vi) was decreased compared to cells grown in the absence of uranium. western analysis did not detect cytochrome c(3) in periplasmic extracts from cells grown in the presence of uranium. the expression of this predominant tetraheme cytochrome w ... | 2004 | 15114437 |
| uranium immobilization by sulfate-reducing biofilms. | hexavalent uranium [u(vi)] was immobilized using biofilms of the sulfate-reducing bacterium (srb) desulfovibrio desulfuricans g20. the biofilms were grown in flat-plate continuous-flow reactors using lactate as the electron donor and sulfate as the electron acceptor. u(vi)was continuously fed into the reactor for 32 weeks at a concentration of 126 microm. during this time, the soluble u(vi) was removed (between 88 and 96% of feed) from solution and immobilized in the biofilms. the dynamics of u ... | 2004 | 15112808 |
| modeling electron transfer thermodynamics in protein complexes: interaction between two cytochromes c(3). | redox protein complexes between type i and type ii tetraheme cytochromes c(3) from desulfovibrio vulgaris hildenborough are here analyzed using theoretical methodologies. various complexes were generated using rigid-body docking techniques, and the two lowest energy complexes (1 and 2) were relaxed using molecular dynamics simulations with explicit solvent and subjected to further characterization. complex 1 corresponds to an interaction between hemes i from both cytochromes c(3). complex 2 corr ... | 2004 | 15111396 |
| cyclic voltammetry and voltabsorptometry studies of redox proteins immobilised on nanocrystalline tin dioxide electrodes. | protein film cyclic voltammetry is a well-established technique for the study of redox proteins immobilised on electrode surfaces. in this paper, we use nanostructured sno(2) electrodes to demonstrate that cyclic voltabsorptometry is an effective, complimentary approach to such studies of protein redox function. we exemplify this approach using two different redox systems: microperoxidase-11 (mp-11) and flavodoxin desulfovibrio vulgaris hildenborough (fld). both systems were immobilised on nanoc ... | 2004 | 15110248 |
| genomic insights into gene regulation of desulfovibrio vulgaris hildenborough. | traditional laboratory studies of the sulfate-reducing bacteria have focused primarily on the biochemistry of the organisms. as genomic sequences of sulfate-reducing species have become available, insights have been gained into the metabolic and regulatory networks of these organisms. a computational analysis is reported of the transcriptional regulatory networks of desulfovibrio vulgaris hildenborough, the first mesophilic gram-negative sulfate-reducing bacterium for which a genome sequence is ... | 2004 | 15107236 |
| crystallization and preliminary x-ray diffraction analysis of the 16-haem cytochrome of desulfovibrio gigas. | high-molecular-weight cytochromes (hmcs) belong to a large family of multihaem cytochromes in sulfate-reducing bacteria. hmca is the first cytochrome reported to have 16 c-type haems arranged in its polypeptide chain. the function of this cytochrome is still unknown, although it is clear that it belongs to a membrane-bound complex involved in electron transfer from the periplasm to the membrane. hmca from desulfovibrio gigas has been purified and successfully crystallized using the hanging-drop ... | 2004 | 15103155 |
| crystal structure of chorismate synthase: a novel fmn-binding protein fold and functional insights. | chorismate synthase catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate to chorismate in the shikimate pathway, which represents an attractive target for discovering antimicrobial agents and herbicides. chorismate serves as a common precursor for the synthesis of aromatic amino acids and many aromatic compounds in microorganisms and plants. chorismate synthase requires reduced fmn as a cofactor but the catalyzed reaction involves no net redox change. here, we have determined the cryst ... | 2004 | 15095868 |
| downcore sulphur isotope ratios and diatom inferred ph in an artificially acidified canadian shield lake. | three gravity cores were removed from near the deepest point in lake 223 on 9 june 1984, eight years after the experimental lakes area (ela) staff began the artificial acidification of the lake with sulphuric acid. the first of these cores was analysed for diatoms and pollen stratigraphy while the second and third were analysed for downcore sulphur isotope ratios (h. thode) and downcore changes in sulphur reducing bacterial densities (s. rao). sediment core chronologies were based on lead-210 an ... | 1988 | 15092659 |
| the genome sequence of the anaerobic, sulfate-reducing bacterium desulfovibrio vulgaris hildenborough. | desulfovibrio vulgaris hildenborough is a model organism for studying the energy metabolism of sulfate-reducing bacteria (srb) and for understanding the economic impacts of srb, including biocorrosion of metal infrastructure and bioremediation of toxic metal ions. the 3,570,858 base pair (bp) genome sequence reveals a network of novel c-type cytochromes, connecting multiple periplasmic hydrogenases and formate dehydrogenases, as a key feature of its energy metabolism. the relative arrangement of ... | 2004 | 15077118 |
| quantification of gram-negative sulphate-reducing bacteria in rice field soil by 16s rrna gene-targeted real-time pcr. | for the quantification of gram-negative sulphate reducers in rice fields, 11 real-time pcr assays were established targeting 16s rrna genes combined with sybrgreen detection. three of these assays were specific for the "main" groups, i.e. the desulfovibrionaceae, the desulfobacteraceae and desulfobulbus sp., whereas eight assays were developed for subgroups within the first two main groups. the detection limits of the assays were between 2 x 10(5) and 4 x 10(3) targets g(-1) (wet weight) or less ... | 2004 | 15063062 |
| time-resolved laser fluorescence spectroscopy study on the interaction of curium(iii) with desulfovibrio äspöensis dsm 10631t. | the influence of microorganisms on migration processes of actinides has to be taken into account for the risk assessment of potential high-level nuclear waste disposal sites. therefore it is necessary to characterize the actinide-bacteria species formed and to elucidate the reaction mechanisms involved. this work is focused on the sulfate-reducing bacterial (srb) strain desulfovibrio äspöensis (d. äspöensis) dsm 10631t which frequently occurs in the deep granitic rock aquifers at the aspö hard r ... | 2004 | 15046347 |
| direct electrochemistry of the desulfovibrio gigas aldehyde oxidoreductase. | this work reports on the direct electrochemistry of the desulfovibrio gigas aldehyde oxidoreductase (dgaor), a molybdenum enzyme of the xanthine oxidase family that contains three redox-active cofactors: two [2fe-2s] centers and a molybdopterin cytosine dinucleotide cofactor. the voltammetric behavior of the enzyme was analyzed at gold and carbon (pyrolytic graphite and glassy carbon) electrodes. two different strategies were used: one with the molecules confined to the electrode surface and a s ... | 2004 | 15030483 |
| x-ray crystal structure of desulfovibrio vulgaris rubrerythrin with zinc substituted into the [fe(scys)4] site and alternative diiron site structures. | the x-ray crystal structure of recombinant desulfovibrio vulgaris rubrerythrin (rbr) that was subjected to metal constitution first with zinc and then iron, yielding zns(4)rbr, is reported. a [zn(scys)(4)] site with no iron and a diiron site with no appreciable zinc in zns(4)rbr were confirmed by analysis of the anomalous scattering data. partial reduction of the diiron site occurred during the synchrotron x-ray irradiation at 95 k, resulting in two different diiron site structures in the zns(4) ... | 2004 | 15023070 |
| identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction. | desulfovibrios produce sulphide, which is toxic to colonic epithelial cells. these bacteria have previously been linked to ulcerative colitis. traditional methods of culturing these organisms are slow, and often unreliable, while molecular approaches are either non-quantitative or lack sensitivity. | 2004 | 15016746 |
| differential expression of desulfovibrio vulgaris genes in response to cu(ii) and hg(ii) toxicity. | the response of desulfovibrio vulgaris to cu(ii) and hg(ii) was characterized. both metals increased the lag phase, and cu(ii) reduced cell yield at concentrations as low as 50 microm. mrna expression was analyzed using random arbitrarily primed pcr, differential display, and quantitative pcr. both cu(ii) and hg(ii) (50 micro m) caused upregulation of mrna expression for an atp binding protein (orf2004) and an atpase (orf856) with four- to sixfold increases for hg(ii) and 1.4- to 3-fold increase ... | 2004 | 15006815 |
| iron corrosion by novel anaerobic microorganisms. | corrosion of iron presents a serious economic problem. whereas aerobic corrosion is a chemical process, anaerobic corrosion is frequently linked to the activity of sulphate-reducing bacteria (srb). srb are supposed to act upon iron primarily by produced hydrogen sulphide as a corrosive agent and by consumption of 'cathodic hydrogen' formed on iron in contact with water. among srb, desulfovibrio species--with their capacity to consume hydrogen effectively--are conventionally regarded as the main ... | 2004 | 14985759 |
| crystallization and mad data collection of high-molecular weight cytochrome c from desulfovibrio vulgaris miyazaki f. | hexadecaheme high molecular weight cytochrome c from a sulfate-reducing bacterium, desulfovibrio vulgaris miyazaki f has been successfully purified and crystallized. x-ray diffraction data have been collected by the multiple wavelength anomalous dispersion method. the crystal belongs to the space group p2(1)2(1)2(1) with unit-cell parameters a=60.42, b=84.29 and c=144.16 a and contains one molecule per asymmetric unit. | 2004 | 14965285 |
| microbial communities and exopolysaccharides from polynesian mats. | microbial mats present in two shallow atolls of french polynesia were characterized by high amounts of exopolysaccharides associated with cyanobacteria as the predominating species. cyanobacteria were found in the first centimeters of the gelatinous mats, whereas deeper layers showing the occurrence of the sulfate reducers desulfovibrio and desulfobacter species as determined by the presence of specific biomarkers. exopolysaccharides were extracted from these mats and partially characterized. al ... | 2001 | 14961381 |
| sulphate-reducing and hydrocarbon-producing bacteria in groundwater. | 1951 | 14933033 | |
| competitive and noncompetitive inhibitors of bacterial sulphate reduction. | 1952 | 14927859 | |
| the reduction of sulphur compounds by desulphovibrio desulphuricans. | 1951 | 14908011 | |
| on the nutrition of desulphovibrio desulphuricans. | 1951 | 14908010 | |
| the role of hydrogenase in the autotrophy of desulphovibrio. | 1951 | 14844833 | |
| [comparative investigation on the growth of sporovibrio desulfuricans on pyruvate and on sodium lactate]. | 1951 | 14829932 | |
| contribution of the [feii(scys)4] site to the thermostability of rubredoxins. | the thermostabilities of fe(2+) ligation in rubredoxins (rds) from the hyperthermophile pyrococcus furiosus (pf) and the mesophiles clostridium pasteurianum (cp) and desulfovibrio vulgaris (dv) were compared. residue 44 forms an nh.s(cys) hydrogen bond to one of the cysteine ligands to the [fe(scys)(4)] site, and substitutions at this location affect the redox properties of the [fe(scys)(4)] site. both pf rd and dv rd have an alanine residue at position 44, whereas cp fd has a valine residue. wi ... | 2004 | 14770302 |
| [analysis of the anaerobic microbial community capable of degrading p-toluene sulphonate]. | three strains of clostridium sp., 14 (vkm b-2201), 42 (vkm b-2202), and 21 (vkm b-2279), two methanogens, methanobacterium formicicum mh (vkm b-2198) and methanosarcina mazei mm (vkm b-2199), and one sulfate-reducing bacterium, desulfovibrio sp. sr1 (vkm b-2200), were isolated in pure cultures from an anaerobic microbial community capable of degrading p-toluene sulfonate. strain 14 was able to degrade p-toluene sulfonate in the presence of yeast extract and bactotryptone and, like strain 42, to ... | 2003 | 14768540 |
| detection and characterization of a dehalogenating microorganism by terminal restriction fragment length polymorphism fingerprinting of 16s rrna in a sulfidogenic, 2-bromophenol-utilizing enrichment. | terminal restriction fragment length polymorphism analysis of reverse-transcribed 16s rrna during periods of community flux was used as a tool to delineate the roles of the members of a 2-bromophenol-degrading, sulfate-reducing consortium. starved, washed cultures were amended with 2-bromophenol plus sulfate, 2-bromophenol plus hydrogen, phenol plus sulfate, or phenol with no electron acceptor and were monitored for substrate use. in the presence of sulfate, 2-bromophenol and phenol were complet ... | 2004 | 14766602 |
| stable carbon isotope ratios of lipid biomarkers of sulfate-reducing bacteria. | we examined the potential use of natural-abundance stable carbon isotope ratios of lipids for determining substrate usage by sulfate-reducing bacteria (srb). four srb were grown under autotrophic, mixotrophic, or heterotrophic growth conditions, and the delta13c values of their individual fatty acids (fa) were determined. the fa were usually 13c depleted in relation to biomass, with deltadelta13c(fa - biomass) of -4 to -17 per thousand; the greatest depletion occurred during heterotrophic growth ... | 2004 | 14766550 |
| a novel approach to investigate biofilm accumulation and bacterial transport in porous matrices. | knowledge of bacterial transport through, and biofilm growth in, porous media is vitally important in numerous natural and engineered environments. despite this, porous media systems are generally oversimplified and the local complexity of cell transport, biofilm formation and the effect of biofilm accumulation on flow patterns is lost. in this study, cells of the sulphate-reducing bacterium, desulfovibrio sp. ex265, accumulated primarily on the leading faces of obstructions and developed into b ... | 2004 | 14756882 |
| enzymatic mechanism of fe-only hydrogenase: density functional study on h-h making/breaking at the diiron cluster with concerted proton and electron transfers. | the mechanism of the enzymatic hydrogen bond forming/breaking (2h(+) + 2e<==>h(2)) and the plausible charge and spin states of the catalytic diiron subcluster [fefe](h) of the h cluster in fe-only hydrogenases are probed computationally by the density functional theory. it is found that the active center [fefe](h) can be rationally simulated as [[h](ch(3)s)(co)(cn(-))fe(p)(co(b))(mu-srs)fe(d)(co)(cn(-))l], where the monovalence [h] stands for the [4fe4s](h)(2+) subcluster bridged to the [fefe](h ... | 2004 | 14753812 |
| cloning and expression of the enolase gene from desulfovibrio vulgaris (miyazaki f). | the gene encoding an enolase from desulfovibrio vulgaris (miyazaki f) was cloned and overexpressed in escherichia coli. a 2.1-kb dna fragment, isolated from d. vulgaris (miyazaki f) by double digestion with psti and bamhi, contained an enolase gene (eno) and part of the methylenetetrahydrofolate dehydrogenase gene (fold). the nucleotide sequence of eno indicates that the protein monomer is composed of 434 amino acids. an expression system for eno under control of the t7 promoter was constructed ... | 2004 | 14746912 |
| thermodesulfatator indicus gen. nov., sp. nov., a novel thermophilic chemolithoautotrophic sulfate-reducing bacterium isolated from the central indian ridge. | a thermophilic, marine, anaerobic, chemolithoautotrophic, sulfate-reducing bacterium, strain cir29812t, was isolated from a deep-sea hydrothermal vent site at the kairei vent field on the central indian ridge. cells were gram-negative motile rods that did not form spores. the temperature range for growth was 55-80 degrees c, with an optimum at 70 degrees c. the nacl concentration range for growth was 10-35 g l(-1), with an optimum at 25 g l(-1). the ph range for growth was 6-6.7, with an optimum ... | 2004 | 14742485 |
| superoxide reductase from desulfoarculus baarsii: identification of protonation steps in the enzymatic mechanism. | superoxide reductase (sor) is a metalloenzyme that catalyzes the reduction of o2*- to h2o2 and provides an antioxidant mechanism in some anaerobic and microaerophilic bacteria. its active site contains an unusual mononuclear ferrous center (center ii). protonation processes are essential for the reaction catalyzed by sor, since two protons are required for the formation of h2o2. we have investigated the acido-basic and ph dependence of the redox properties of the active site of sor from desulfoa ... | 2004 | 14730986 |
| [methods for estimation of microbe resistance of protective coatings]. | methods are proposed which permit estimating bioresistance of protective materials against denitrifying hydrocarbon-oxidizing and sulphate-reducing bacteria-destructors of coatings in laboratory conditions. the methods are based on the quantitative determination of bacteria growth in presence of coatings as the only source of hydrocarbon. besides, attention is given to changes of certain indices of physico-mechanical properties of coatings: breaking strength and adhesion strength for coatings fr ... | 2003 | 14723162 |
| periplasmic cytochrome c3 of desulfovibrio vulgaris is directly involved in h2-mediated metal but not sulfate reduction. | kinetic parameters and the role of cytochrome c(3) in sulfate, fe(iii), and u(vi) reduction were investigated in desulfovibrio vulgaris hildenborough. while sulfate reduction followed michaelis-menten kinetics (k(m) = 220 micro m), loss of fe(iii) and u(vi) was first-order at all concentrations tested. initial reduction rates of all electron acceptors were similar for cells grown with h(2) and sulfate, while cultures grown using lactate and sulfate had similar rates of metal loss but lower sulfa ... | 2004 | 14711670 |
| molecular basis for redox-bohr and cooperative effects in cytochrome c3 from desulfovibrio desulfuricans atcc 27774: crystallographic and modeling studies of oxidized and reduced high-resolution structures at ph 7.6. | the tetraheme cytochrome c3 is a small metalloprotein with ca. 13,000 da found in sulfate-reducing bacteria, which is believed to act as a partner of hydrogenase. the three-dimensional structure of the oxidized and reduced forms of cytochrome c3 from desulfovibrio desulfuricans atcc 27774 at ph 7.6 were determined using high-resolution x-ray crystallography and were compared with the previously determined oxidized form at ph 4.0. theoretical calculations were performed with both structures, usin ... | 2004 | 14705030 |
| improvement of comparative modeling by the application of conserved motifs amongst distantly related proteins as additional restraints. | protein comparative modeling has useful applications in large-scale structural initiatives and in rational design of drug targets in medicinal chemistry. the reliability of a homology model is dependent on the sequence identity between the query and the structural homologue used as a template for modeling. here, we present a method for the utilization and conservation of important structural features of template structures by providing additional spatial restraints in comparative modeling progra ... | 2004 | 14691673 |
| a glutamate is the essential proton transfer gate during the catalytic cycle of the [nife] hydrogenase. | kinetic, epr, and fourier transform infrared spectroscopic analysis of desulfovibrio fructosovorans [nife] hydrogenase mutants targeted to glu-25 indicated that this amino acid participates in proton transfer between the active site and the protein surface during the catalytic cycle. replacement of that glutamic residue by a glutamine did not modify the spectroscopic properties of the enzyme but cancelled the catalytic activity except the para-h(2)/ortho-h(2) conversion. this mutation impaired t ... | 2004 | 14688251 |
| incorporation of either molybdenum or tungsten into formate dehydrogenase from desulfovibrio alaskensis ncimb 13491; epr assignment of the proximal iron-sulfur cluster to the pterin cofactor in formate dehydrogenases from sulfate-reducing bacteria. | we report the characterization of the molecular properties and epr studies of a new formate dehydrogenase (fdh) from the sulfate-reducing organism desulfovibrio alaskensis ncimb 13491. fdhs are enzymes that catalyze the two-electron oxidation of formate to carbon dioxide in several aerobic and anaerobic organisms. d. alaskensis fdh is a heterodimeric protein with a molecular weight of 126+/-2 kda composed of two subunits, alpha=93+/-3 kda and beta=32+/-2 kda, which contains 6+/-1 fe/molecule, 0. ... | 2004 | 14669076 |
| desulfovibrio desulfuricans lipopolysaccharides induce endothelial cell il-6 and il-8 secretion and e-selectin and vcam-1 expression. | the aim of this study was to determine whether desulfovibrio desulfuricans-derived lps stimulate the release of il-6 and il-8 from ecs and the expression of their adhesion molecules at the transcriptional level. confluent monolayers of huvec were incubated in the absence or presence of 20 microg/ml and 60 microg/ml lpss derived from the ddt and dda bacterial strains. also, the simultaneous stimulation of cells with lpss and il-1beta was evaluated. the levels of cytokines released were measured u ... | 2003 | 14668922 |
| x-ray induced reduction of the crystal of high-molecular-weight cytochrome c revealed by microspectrophotometry. | the crystal structures of high-molecular-weight cytochrome c (hmc) from desulfovibrio vulgaris hildenborough in the transient and reduced states have been determined at 2.8 a resolution. an absorption spectrum measured with microspectrophotometer indicated that about 86% of the hemes were reduced after 45 min irradiation of the x-ray beam. further exposure for 90 min did not significantly change the spectrum. these results suggest that hmc in the crystalline state is easily reduced by illuminati ... | 2004 | 14646149 |
| crystallization and preliminary neutron analysis of the dissimilatory sulfite reductase d (dsrd) protein from the sulfate-reducing bacterium desulfovibrio vulgaris. | dissimilatory sulfite reductase d (dsrd) from desulfovibrio vulgaris has been crystallized for a neutron diffraction study. the initial crystals obtained were too small for the neutron experiment. in order to obtain a larger crystal (>1 mm3), a combination of two techniques was developed to determine the optimum crystallization conditions: a crystallization phase diagram was obtained, followed by crystal-quality assessment via x-ray diffraction. using conditions determined in this manner, a larg ... | 2003 | 14646103 |
| structure of the hybrid cluster protein (hcp) from desulfovibrio desulfuricans atcc 27774 containing molecules in the oxidized and reduced states. | the hybrid cluster protein (hcp) from the sulfate-reducing bacteria desulfovibrio desulfuricans atcc 27774 has been isolated and crystallized anaerobically. the protein sample used in the crystallization studies was several months old, having been stored at 193 k, and initial crystal structure studies were unable to fully resolve details of the hybrid cluster despite the use of high-resolution data to 1.25 a collected at the esrf, grenoble, france. full elucidation of the structure has only beco ... | 2003 | 14646063 |
| protein stabilization by compatible solutes. effect of diglycerol phosphate on the dynamics of desulfovibrio gigas rubredoxin studied by nmr. | heteronuclear nmr relaxation measurements and hydrogen exchange data have been used to characterize protein dynamics in the presence or absence of stabilizing solutes from hyperthermophiles. rubredoxin from desulfovibrio gigas was selected as a model protein and the effect of diglycerol phosphate on its dynamic behaviour was studied. the presence of 100 mm diglycerol phosphate induces a fourfold increase in the half-life for thermal denaturation of d. gigas rubredoxin. a model-free analysis of t ... | 2003 | 14622247 |
| mutual influences of pseudomonas aeruginosa and desulfovibrio desulfuricans on their adhesion to stainless steel. | the mutual influences of pseudomonas aeruginosa pao1 and desulfovibrio desulfuricans subsp. desulfuricans (atcc 29577) on their adhesion to stainless steel were investigated in batch and column experiments. it was found that p. aeruginosa promoted the adhesion of d. desulfuricans under conditions of turbulence, but not under quiescent conditions. the enhancement involved the alignment of most d. desulfuricans along p. aeruginosa cells and was attributed to the additional interaction surface area ... | 2003 | 14618696 |
| adhesion of anaerobic microorganisms to solid surfaces and the effect of sequential attachment on adhesion characteristics. | the attachment of three anaerobic microorganisms, desulfomonile tiedjei, syntrophomonas wolfei, and desulfovibrio sp. strain g11, was investigated to determine if the presence of one species could influence the adhesion of another species to glass surfaces. the results indicated that the numbers and distribution of attached cells of one species could be influenced considerably by the presence of another species and the order in which the test species were exposed to the surface. d. tiedjei was f ... | 2003 | 14618685 |
| can cofactor-binding sites in proteins be flexible? desulfovibrio desulfuricans flavodoxin binds fmn dimer. | flavodoxins catalyze redox reactions using the isoalloxazine moiety of the flavin mononucleotide (fmn) cofactor stacked between two aromatic residues located in two peptide loops. at high fmn concentrations that favor stacked fmn dimers in solution, isothermal titration calorimetric studies show that these dimers bind strongly to apo-flavodoxin from desulfovibrio desulfuricans (30 degrees c, 20 mm hepes, ph 7, k(d) = 5.8 microm). upon increasing the temperature so the fmn dimers dissociate (as s ... | 2003 | 14596623 |
| a new function of the desulfovibrio vulgaris hildenborough [fe] hydrogenase in the protection against oxidative stress. | sulfate-reducing bacteria, like desulfovibrio vulgaris hildenborough, have developed a set of reactions allowing them to survive in oxic environments and even to reduce molecular oxygen to water. d. vulgaris contains a cytoplasmic superoxide reductase (sor) and a periplasmic superoxide dismutase (sod) involved in the elimination of superoxide anions. to assign the function of sod, the periplasmic [fe] hydrogenase activity was followed in both wild-type and sod deletant strains. this activity was ... | 2004 | 14594815 |
| role of the aromatic ring of tyr43 in tetraheme cytochrome c(3) from desulfovibrio vulgaris miyazaki f. | tyrosine 43 is positioned parallel to the fifth heme axial ligand, his34, of heme 1 in the tetraheme cytochrome c(3). the replacement of tyrosine with leucine increased the redox potential of heme 1 by 44 and 35 mv at the first and last reduction steps, respectively; its effects on the other hemes are small. in contrast, the y43f mutation hardly changed the potentials. it shows that the aromatic ring at this position contributes to lowering the redox potential of heme 1 locally, although this ca ... | 2003 | 14581238 |
| direct detection of a hydrogen ligand in the [nife] center of the regulatory h2-sensing hydrogenase from ralstonia eutropha in its reduced state by hyscore and endor spectroscopy. | the regulatory h2-sensing [nife] hydrogenase of the beta-proteobacterium ralstonia eutropha displays an ni-c "active" state after reduction with h2 that is very similar to the reduced ni-c state of standard [nife] hydrogenases. pulse electron nuclear double resonance (endor) and four-pulse eseem (hyperfine sublevel correlation, hyscore) spectroscopy are applied to obtain structural information on this state via detection of the electron-nuclear hyperfine coupling constants. two proton hyperfine ... | 2003 | 14570480 |
| crystal structure of sulerythrin, a rubrerythrin-like protein from a strictly aerobic archaeon, sulfolobus tokodaii strain 7, shows unexpected domain swapping. | sulerythrin is the first rubrerythrin-like protein to be isolated from an aerobic organism, sulfolobus tokodaii strain 7, and it lacks a c-terminal rubredoxin-like fes(4) domain. the protein purified from sulfolobus cells was crystallized, and the crystal structure was determined at 1.7 a resolution. the dimer of sulerythrin exhibited "domain-swapping" at the loop connecting alphab and alphac, hybrid four-helix bundles consisting of alphaa/b and alphac/d being formed. the structure and atomic id ... | 2003 | 14529281 |
| mutagenesis study on the role of a lysine residue highly conserved in formate dehydrogenases and periplasmic nitrate reductases. | lysine 85 (k85) in the primary structure of the catalytic subunit of the periplasmic nitrate reductase (nap-a) of ralstonia eutropha h16 is highly conserved in periplasmic nitrate reductases and in the structurally related catalytic subunit of the formate dehydrogenases of various bacterial species. it is located between an [4fe-4s] center and one of the molybdopterin-guanine dinucleotides mediating the through bonds electron flow to convert the specific substrate of the respective enzymes. to e ... | 2003 | 14511645 |
| the isolation and characterization of cytochrome c nitrite reductase subunits (nrfa and nrfh) from desulfovibrio desulfuricans atcc 27774. re-evaluation of the spectroscopic data and redox properties. | the cytochrome c nitrite reductase is isolated from the membranes of the sulfate-reducing bacterium desulfovibrio desulfuricans atcc 27774 as a heterooligomeric complex composed by two subunits (61 kda and 19 kda) containing c-type hemes, encoded by the genes nrfa and nrfh, respectively. the extracted complex has in average a 2nrfa:1nrfh composition. the separation of ccnir subunits from one another is accomplished by gel filtration chromatography in the presence of sds. the amino-acid sequence ... | 2003 | 14511372 |
| sediment microbial community composition and methylmercury pollution at four mercury mine-impacted sites. | mercury pollution presents a globally significant threat to human and ecosystem health. an important transformation in the mercury cycle is the conversion of inorganic mercury to methylmercury, a toxic substance that negatively affects neurological function and bioaccumulates in food chains. this transformation is primarily bacterially mediated, and sulfate-reducing bacteria (srb) have been specifically implicated as key mercury methylators in lake and estuarine sediments. this study used phosph ... | 2003 | 14502415 |
| the role of adenosine-5'-phosphosulfate in the reduction of sulfate to sulfite by desulfovibrio desulfuricans. | 1962 | 14484820 | |
| enzymatic basis for assimilatory and dissimilatory sulfate reduction. | peck, h. d., jr. (oak ridge national laboratory, oak ridge, tenn.). enzymatic basis for assimilatory and dissimilatory sulfate reduction. j. bacteriol. 82: 933-939. 1961.-two pathways for the reduction of sulfate to sulfite in bacteria have been previously described. the substrate for sulfate reduction by extracts of yeast is 3'-phosphoadenosine-5'-phosphosulfate (paps) and, in contrast, the substrate for sulfate reduction in extracts of desulfovibrio desulfuricans is adenosine-5'-phosphosulfate ... | 1961 | 14484818 |
| purification & propertis of sulfurylase from desulovibrio desulfuricans. | 1961 | 14448443 | |
| a search for the rubentschikii group of desulphovibrio. | 1959 | 14444778 | |
| the purification of hydrogenase of desulfovibrio desulfuricans. | 1959 | 14440671 | |
| the economic activities of sulphate-reducing bacteria. | 1960 | 14434409 | |
| evidence for oxidative phosphorylation during the reduction of sulfate with hydrogen by desulfovibrio desulfuricans. | 1960 | 14431287 | |
| [influence of nitrogen fixation on the growth of desulfovibrio desulfuricans]. | 1960 | 14414884 | |
| the purification and properties of the hydrogenase of desulfovibrio desulfuricans. | 1960 | 14411716 | |
| [microbiological problems in the etiology of endemic goiter]. | 1955 | 14396356 | |
| the metabolism of malate and certain other compounds by desulphovibrio desulphuricans. | 1955 | 14392298 | |
| [chemistry of hydrogen reduction of sulfates]. | 1954 | 14373902 | |
| [new methods of isolation of sulfate-reducing bacteria]. | 1952 | 14373895 | |
| [morphologic characteristics of sulfur-reducing bacteria found in oil-bearing sites]. | 1952 | 14373885 | |
| [sulfite as an intermediate in the reduction of sulfate by desulfovibrio desulfuricans]. | 1955 | 14352481 | |
| the production of surface active compounds by micro-organisms and its possible significance in oil recovery ii. on the release of oil from oil-sand mixtures with the aid of sulphate reducing bacteria. | 1955 | 14350597 | |
| the control of sulphate activation in bacteria. | 1. atp-sulphate adenylyltransferase (ec 2.7.7.4) and atp-adenylyl sulphate 3'-phosphotransferase (ec 2.7.1.25) of escherichia coli 9723, e. coli k(12) and bacillus subtilis 1379 are each repressed by growth in the presence of cystine. repression of the two enzymes in e. coli 9723 may be co-ordinate. 2. atp-sulphate adenylyltransferase of desulphovibrio desulphuricans, in which sulphate reduction is linked to the energy supply of the organism, is not repressed by growth in the presence of inorgan ... | 1965 | 14343144 |
| [cytochome c3 of desulfovibrio gigas]. | 1965 | 14336080 | |
| studies on adenosine 5'-phosphosulfate reductase from desulfovibrio desulfuricans and thiobacillus thioparus. i. the assay and purification. | 1965 | 14314382 | |
| [isolation in pure culture of a marine clostridium, agent of anaerobic symbiotic alginolysis]. | 1965 | 14308779 | |
| a sulphate-reducing bacterium containing cytochrome c3 but lacking desulfoviridin. | 1964 | 14250805 | |
| iron requirement for the hydrogenase of desulfovibrio desulfuricans. | 1964 | 14240576 | |
| [on a sulfate reducing bacteria isolated from sheep rumen]. | 1964 | 14221473 | |
| differences in the resistance of sulphate-reducing bacteria to inhibitors. | 1964 | 14215434 | |
| carbon isotope fractionation during metabolism of lactate by desulfovibrio desulfuricans. | 1964 | 14140997 | |
| microbiological fractionation of sulphur isotopes. | 1964 | 14135528 | |
| hydrogen sulphide hazard in saline waters. | 1964 | 14127929 | |
| [research on anaerobic bacterial alginolysis]. | 1963 | 14102050 | |
| a strain of desulfovibrio able to use oxamate. | 1963 | 14092426 | |
| [measurement of bacterial reductase activity]. | 1963 | 14089649 | |
| some observations on the enzyme hydrogenase of desulfovibrio desulfuricans. | 1963 | 14082355 | |
| a new species of desulfovibrio. | 1963 | 14080783 |