Publications

microbiological aspects of vulvovaginitis in prepubertal girls.	this study aimed to establish the vaginal introitus microbial flora in girls with and without symptoms of vulvovaginitis, and to present the distribution of isolated microorganisms by age groups in girls with vulvovaginitis. we enrolled 500 girls with vulvovaginitis symptoms, aged 2-12 years, referred by their pediatricians for microbiological examination of the vaginal introitus swabs, and 30 age-matched asymptomatic girls. similar microbial flora was isolated in both groups, but the symptomati ...	2012	22383074
extended-spectrum β-lactamase (esbl) in danish clinical isolates of escherichia coli and klebsiella pneumoniae: prevalence, β-lactamase distribution, phylogroups, and co-resistance.	most gram-negative community-acquired and nosocomial infections are caused by escherichia coli and klebsiella pneumoniae, among which increasing resistance due to extended-spectrum β-lactamase (esbl) is a major problem. we present data from the first danish nationwide prevalence study on esbl-producing e. coli, k. pneumoniae, and proteus mirabilis in blood and urine cultures from hospitals and the community.	2012	22364227
current studies on bacterospermia the leading cause of male infertility: a protégé and potential threat towards mans extinction.	the current rise of male infertility associated with bacterospermia and urogenital infection has been on the increase amongst adult married males in benin metropolis and a major cause of concern to male fertility and reproduction in nigeria.	2011	22363079
antimicrobial activities against biofilm formed by proteus mirabilis isolates from wound and urinary tract infections.	bacterial species are capable of living as biofilm and/or planktonic forms. there is increasing evidence for the role of bacterial biofilm in various wound and urinary tract infections (utis). the aim of the present study was to evaluate the ability of the bacteria, isolated from urinary tract infections (utis) and wound infections, to form biofilm and correlate the role of biofilm with their antimicrobial resistance.	2012	22361765
antiadhesive activity of the biosurfactant pseudofactin ii secreted by the arctic bacterium pseudomonas fluorescens bd5.	pseudofactin ii is a recently identified biosurfactant secreted by pseudomonas fluorescens bd5, the strain obtained from freshwater from the arctic archipelago of svalbard. pseudofactin ii is a novel compound identified as cyclic lipopeptide with a palmitic acid connected to the terminal amino group of eighth amino acid in peptide moiety. the c-terminal carboxylic group of the last amino acid forms a lactone with the hydroxyl of thr3. adhesion is the first stage of biofilm formation and the best ...	2012	22360895
emergence of proteus mirabilis harboring blakpc-2 and qnrd in a chinese hospital.	nineteen carbapenem-nonsusceptible proteus mirabilis isolates were recovered from intensive care units in the second affiliated hospital of zhejiang university during a 3-month period. the isolates showed a high level of resistance against ciprofloxacin, in addition to their resistance against the carbapenems. pulsed-field gel electrophoresis (pfge) analysis showed that these isolates belonged to three clonal strains. pcrs and dna sequence analysis of the carbapenemase and other β-lactamase gene ...	2012	22354308
bacteremia in a long term care facility.	episodes of bacteremia identified in a long term care facility over a seven and a half-year period from july 1984 to december 1991 were reviewed. twenty-nine episodes of bacteremia were identified, a rate of 4.35/100,000 patient-days. the most common infecting organisms were escherichia coli (11 episodes), streptococcus pneumoniae (four), proteus mirabilis (three), staphylococcus aureus (three) and bacteroides species (two). the source of bacteremia was urinary in 45% of patients, gastrointestin ...	1994	22346488
concurrent neonatal proteus mirabilis infection in dizygotic twins.	a case of concomitant infection with proteus mirabilis in dizygotic twin neonates is presented. the first twin presented with meningitis and septic shock at eight days of age and subsequently died. an investigation of the asymptomatic second twin revealed a urinary tract infection that resolved with antimicrobial therapy. it is recommend that when infection with this virulent organism is diagnosed in one twin, the second twin should be fully evaluated for sepsis and empirical antimicrobial thera ...	1999	22346381
extensive, non-healing scalp ulcer associated with trauma-induced chronic osteomyelitis.	a 77-year-old woman presented with a trauma to the scalp caused from the blade of a windmill. the condition was persistent from the past 50 years. at the initial examination, a deep, foul-smelling and well-circumscribed ulcer was apparent on the head region, involving the majority of the cranium. skin biopsy specimens of the lesion were nonspecific. the bone biopsy showed extensive necrotic areas of bone and soft tissues, with lymphocytic exudate foci. a computed tomography scan of the head reve ...	2011	22346279
ion mobility spectrometry for microbial volatile organic compounds: a new identification tool for human pathogenic bacteria.	presently, 2 to 4 days elapse between sampling at infection suspicion and result of microbial diagnostics. this delay for the identification of pathogens causes quite often a late and/or inappropriate initiation of therapy for patients suffering from infections. bad outcome and high hospitalization costs are the consequences of these currently existing limited pathogen identification possibilities. for this reason, we aimed to apply the innovative method multi-capillary column-ion mobility spect ...	2012	22327321
using nucleic acid microarrays to perform molecular epidemiology and detect novel β-lactamases: a snapshot of extended-spectrum β-lactamases throughout the world.	the worldwide dissemination of extended-spectrum-β-lactamase (esbl)- and carbapenemase-producing enterobacteriaceae is a major concern in both hospital and community settings. rapid identification of these resistant pathogens and the genetic determinants they possess is needed to assist in clinical practice and epidemiological studies. a collection of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, and proteus mirabilis isolates, including phenotypically esbl-positive (n = 1,093) an ...	2012	22322349
rapid detection of urinary tract pathogens using microcalorimetry: principle, technique and first results.	what's known on the subject? and what does the study add? microcalorimetry has been applied in several microbiological studies, but never in a clinical urological context. in addition, basic knowledge on the growth of urinary pathogens in urine is still scarce and data regarding the growth rate of many urinary pathogens in urine are still not available. the study demonstrates that this innovative application of microcalorimetry is useful in (i) investigating the growth of urinary pathogens in st ...	2012	22313675
biodegradation of organic compounds of molasses melanoidin (mm) from biomethanated distillery spent wash (bmds) during the decolourisation by a potential bacterial consortium.	molasses melanoidin (mm) is a major pollutant in biomethanated distillery spent wash (bmds) due to its recalcitrant properties. the 75% colour and 71% cod of mm (1,000 ppm) were reduced with developed bacterial consortium comprising proteus mirabilis (iitrm5; fj581028), bacillus sp. (iitrm7; fj581030), raoultella planticola (iitrm15; gu329705) and enterobacter sakazakii (iitrm16, fj581031) in the ratio of 4:3:2:1 within 10 days at optimized nutrient. bacterial consortium showed manganese peroxid ...	2012	22311589
broad identification of bacterial type in urinary tract infection using (1)h nmr spectroscopy.	to address the shortcomings of urine culture for the rapid identification of urinary tract infection (uti), we applied (1)h-nuclear magnetic resonance (nmr) spectroscopy as a surrogate method for fast screening of microorganisms. study includes 682 urine samples from suspected uti patients, 50 healthy volunteers, and commercially available standard strains of gram negative bacilli (gnb) (escherichia coli, pseudomonas aeruginosa, klebsiella pneumonia, enterobacter, acinetobacter, proteus mirabili ...	2012	22292465
vaginal bacterial flora and cytology in proestrous bitches: role on fertility.	the study of canine vaginal cytology underwent limited evolution over the years. presence and significance of inflammatory cells in vaginal smears are little considered aspects in the bitch. moreover, occurrence of vaginal bacteria in breeding bitches during follicular phase of the reproductive cycle, in absence of clinical signs of infection, involves the difficult question of antibiotics administration. the aim of this study was to relate findings in vaginal cytology (presence of neutrophils, ...	2012	22289216
[epidemiology and clinical features in diabetic patients with invasive infections treated at the clinical hospital of infectious diseases of iaşi, romania, between 2008-2010].	infections in the diabetic population can be severe and life threatning at least for two reasons: clinical signs are often torpid, masqued by chronical complications of diabetes leading to late recognition and medical adresability and also because of the inability to control established infection due to complex cell-mediated and humoral immunity deffects. the aim of the study was to evaluate the etiology, clinical features and outcome in diabetic patients with invazive disease.	2011	22276442
post-operative wound infection in salvage laryngectomy: does antibiotic prophylaxis have an impact?	salvage laryngectomy carries a high risk of post-operative infection with reported rates of 40-61%. the purpose of this study was to analyse infections in our own patients and review the potential impact of our current antibiotic prophylaxis (ap). a retrospective analysis of infection in 26 consecutive patients between 2000 and 2010 undergoing salvage total laryngectomy (sl) following recurrent laryngeal cancer after failed radiotherapy or chemo-radiation was undertaken. the antibiotic prophylax ...	2012	22274693
multi-centre evaluation of a phenotypic extended spectrum β-lactamase detection guideline in the routine setting.	this study aimed to evaluate the routine setting performance of a guideline for phenotypic detection of extended spectrum β-lactamases (esbls) in enterobacteriaceae, recommending esbl confirmation with etest or combination disc for isolates with a positive esbl screen test (i.e. cefotaxime and/or ceftazidime mic >1 mg/l or an automated system esbl warning). twenty laboratories submitted 443 enterobacteriaceae with a positive esbl screen test and their confirmation test result (74%escherichia col ...	2013	22268620
french results of the aresc study: clinical aspects and epidemiology of antimicrobial resistance in female patients with cystitis. implications for empiric therapy.	objective: uncomplicated cystitis is one of the most frequent community infections. we report the french results of the international aresc study on the clinical aspects, epidemiology, and antimicrobial susceptibility of uropathogens. patient and methods: female patients between 18 and 65 years of age, with symptoms of uncomplicated cystitis, were investigated clinically with urinalysis and urine culture. uropathogens were identified and their susceptibility was tested with nine antimicrobials. ...	2012	22264668
prevalence of 16s rrna methylase genes in enterobacteriaceae isolates from a greek university hospital.	16s ribosomal rna methylase-mediated high-level resistance to 4-,6-aminoglycosides has been reported in clinical isolates of gram-negative bacilli from several countries. three of 1534 (0.2%) isolates of klebsiella pneumoniae and three of 734 (0.4%) proteus mirabilis isolates from a university hospital in athens, greece, were positive for rmtb and highly resistant to all aminoglycosides tested (mics ≥256 mg/l). two of the k. pneumoniae rmtb-bearing isolates, were kpc-2 and oxa-10 producers and t ...	2012	22264302
proteus mirabilis interkingdom swarming signals attract blow flies.	flies transport specific bacteria with their larvae that provide a wider range of nutrients for those bacteria. our hypothesis was that this symbiotic interaction may depend on interkingdom signaling. we obtained proteus mirabilis from the salivary glands of the blow fly lucilia sericata; this strain swarmed significantly and produced a strong odor that attracts blow flies. to identify the putative interkingdom signals for the bacterium and flies, we reasoned that as swarming is used by this bac ...	2012	22237540
antiquorum sensing and antibiofilm potential of capparis spinosa.	emergence of antibiotic resistance among bacterial pathogens often leads to the failure of existing antibiotics to treat bacterial infections; thus, there is a need to seek alternative treatment measures. the aim of this study was to evaluate the anti-quorum sensing (anti-qs) and antibiofilm potential of capparis spinosa to prevent the onset of bacterial infections as an alternate to antibiotics.	2011	22222491
antimicrobial susceptibility of gram-negative pathogens isolated from patients with complicated intra-abdominal infections in south african hospitals (smart study 2004-2009): impact of the new carbapenem breakpoints.	abstract background: the study for monitoring antimicrobial resistance trends (smart) follows trends in resistance among aerobic and facultative anaerobic gram-negative bacilli (gnb) isolated from complicated intra-abdominal infections (ciais) in patients around the world. methods: during 2004-2009, three centralized clinical microbiology laboratories serving 59 private hospitals in three large south african cities collected 1,218 gnb from complicated intra-abdominal infections (ciais) and t ...	2012	22220506
radial and spiral stream formation in proteus mirabilis colonies.	the enteric bacterium proteus mirabilis, which is a pathogen that forms biofilms in vivo, can swarm over hard surfaces and form a variety of spatial patterns in colonies. colony formation involves two distinct cell types: swarmer cells that dominate near the surface and the leading edge, and swimmer cells that prefer a less viscous medium, but the mechanisms underlying pattern formation are not understood. new experimental investigations reported here show that swimmer cells in the center of the ...	2011	22219724
influence of quorum sensing signal molecules on biofilm formation in proteus mirabilis o18.	the influence of basis of quorum sensing molecules on proteus strains is much less known as compared to pseudomonas or escherichia. we have previously shown that a series of acylated homoserine lactones (acyl-hsl) does not influence the ureolytic, proteolytic, or hemolytic abilities, and that the swarming motility of proteus mirabilis rods is strain specific. the aim of the presented study was to find out if the presence of a series of acyl-hsl influences biofilm formation of p. mirabilis labora ...	2011	22198843
Gram stain microbiological pattern of upper extremities suppuration at Baptist Medical Centre, Ogbomoso Nigeria: a fifteen month review.	Sixty-eight (68) patients with serious upper extremity suppurative infections, presenting within a period of fifteen (15) months, were prospectively studied clinically, Gram stain of aspirates/pus were performed, specimen cultured, planted, and where indicated glucose levels and haemoglobin genotype determined. Half of the patients had hand infections. Staphylococcus aureus was isolated from thirty-nine (39) patients. Gram Negative bacilli, including Salmonella were more isolated from patients w ...	2011	22195385
a self-loading microfluidic device for determining the minimum inhibitory concentration of antibiotics.	this article describes a portable microfluidic technology for determining the minimum inhibitory concentration (mic) of antibiotics against bacteria. the microfluidic platform consists of a set of chambers molded in poly(dimethylsiloxane) (pdms) that are preloaded with antibiotic, dried, and reversibly sealed to a second layer of pdms containing channels that connect the chambers. the assembled device is degassed via vacuum prior to its use, and the absorption of gas by pdms provides the mechani ...	2011	22193301
description and plasmid characterization of qnrd determinants in proteus mirabilis and morganella morganii.	clin microbiol infect abstract: we investigated the presence of qnrc and qnrd among 756 non-replicate enterobacteriaceae isolated in italy, selected for being non-susceptible to fluoroquinolones and/or resistant to third-generation cephalosporins. four proteus mirabilis and one morganella morganii (0.66% of the total) presented a qnrd gene, located in a 2687-base-pair plasmid that was entirely sequenced. the plasmid is un-typable, and contains no known coding region other than qnrd. that the qnr ...	2011	22192340
[the susceptibility of proteus mirabilis strains isolated from white stork (ciconia ciconia)].	proteus sp. rods are ubiquitous bacteria, widespread in the environment and classified also as opportunistic human pathogens. the aim of our study was to evaluate susceptibility of proteus mirabilis strains isolated from white stork (ciconia ciconia) regarding as his natural bacterial flora, compare and discuss their results with data obtained from scientific literature for clinical strains of the same species. susceptibility of 59 p. mirabilis strains was estimated for 27 antimicrobials using d ...	2011	22184908
[evaluation of biofilm formation by proteus mirabilis strains on the surface of different biomaterials by two methods].	proteus sp. rods are opportunistic human pathogens. these microorganisms are mainly isolated from patients with urinary tract infections, particularly associated with using of biomaterials, on which surface they can form biofilm. the aim of our study was the estimation of proteus mirabilis rods ability to form biofilm on the surface of 5 biomaterials (polychloride vinyl, silicone latex, polypropylene, polybutylen teraftalan and polyamide) using richards' and quantitative method and comparison re ...	2011	22184907
Anti-bacterial studies on Hemigraphis colorata (Blume) H.G. Hallier and Elephantopus scaber L.	To examine the ethanol, aqueous, chloroform, benzene, acetone and petroleum ether extracts of, Hemigraphis colorata (H. colorata) leaves and stem and Elephantopus scaber (E. scaber) leaves, root and flower for the presence of phyto-constituents and screened the anti-bacterial activity against the selected pathogens.	2012	22182644
the antimicrobial efficacy of silver on antibiotic-resistant bacteria isolated from burn wounds.	the antibiotic-resistant bacteria are a major concern to wound care because of their ability to resist many of the antibiotics used today to treat infections. consequently, other antimicrobials, in particular ionic silver, are considered ideal topical agents for effectively helping to manage and prevent local infections. little is known about the antimicrobial efficacy of ionic silver on antibiotic-resistant bacteria at different ph values. consequently, in this study our aim was to evaluate the ...	2011	22182219
Antimicrobial susceptibility of Gram-negative organisms from intraabdominal infections and evolution of isolates with extended spectrum ß-lactamases in the SMART study in Spain (2002-2010).	Introduction. The SMART (Study for Monitoring Antimicrobial Resistance Trends) surveillance study records the antimicrobial susceptibility of Gram-negative bacilli obtain from intraabdominal infections with special focus in isolates with extended spectrum ß-lactamases (ESBLs). Material and Methods. The antimicrobial susceptibility of 8,869 isolates was analyzed by microdilution during the SMART study performed in Spain from 2002 to 2010. Isolates were recovered in 16 centres. Results. Escherichi ...	2011	22173194
activities of jatropha curcas phorbol esters in various bioassays.	jatropha curcas seeds contain 30-35% oil, which can be converted to high quality biodiesel. however, jatropha oil is toxic, ascribed to the presence of phorbol esters (pes). in this study, isolated phorbol ester rich fraction (peef) was used to evaluate the activity of pes using three aquatic species based bioassays (snail (physa fontinalis), brine shrimp (artemeia salina), daphnia (daphnia magna)) and microorganisms. in all the bioassays tested, increase in concentration of pes increased mortal ...	2011	22172520
frequency of extended spectrum beta lactamase producing enterobacteriaceae among urinary pathogen isolates.	objective: to determine the frequency of extended-spectrum beta lactamase (esbl) producing enterobacteriaceae in urinary isolates. study design: observational study. place and duration of study: ziauddin university hospital, karachi, from february to october 2008. methodology: all members of enterobacteriaceae isolated from urinary samples of in-patients were included and identified using standard biochemical tests. urinary samples from out-patients were excluded. detection of esbl was carried o ...	2011	22166694
Preventing catheter-related urinary tract infection: focus on Proteus mirabilis.	Urinary tract infectious caused by Proteus are uncommon, and account for only 1-2% of community-acquired infectious. However, it is important that staff are able to recognise the features of a Proteus infection. Proteus infections, if unrecognised, can become established and cause major health problems. Proteus is more common in people who have or have had a urinary catheter. Minimizing the incidence and duration of urinary catheterization is an important part of preventing infection. Community ...	2011	22145192
role of the umo proteins and the rcs phosphorelay in the swarming motility of the wild type and an o-antigen (waal) mutant of proteus mirabilis.	proteus mirabilis is a gram-negative bacterium that exists as a short rod when grown in liquid medium, but during growth on surfaces it undergoes a distinct physical and biochemical change that culminates in the formation of a swarmer cell. how p. mirabilis senses a surface is not fully understood; however, the inhibition of flagellar rotation and accumulation of putrescine have been proposed to be sensory mechanisms. our lab recently isolated a transposon insertion in waal, encoding o-antigen l ...	2011	22139504
colonisation and infection due to enterobacteriaceae producing plasmid-mediated ampc β-lactamases.	to investigate the epidemiology and clinical features of infections caused by enterobacteria producing plasmid-mediated ampc β-lactamases (pampc), which are emerging as a cause of resistance to extended-spectrum cephalosporins.	2011	22138600
macroscopic behavior of systems with an axial dynamic preferred direction.	we present the derivation of the macroscopic equations for systems with an axial dynamic preferred direction. in addition to the usual hydrodynamic variables, we introduce the time derivative of the local preferred direction as a new variable and discuss its macroscopic consequences including new cross-coupling terms. such an approach is expected to be useful for a number of systems for which orientational degrees of freedom are important including, for example, the formation of dynamic macrosco ...	2011	22120542
Acinetobacter baumannii is Able to Gain and Maintain a Plasmid Harbouring In35 Found in Enterobacteriaceae Isolates From Argentina.	The aim of this study was to determine the presence of bla (CTX-M-2) in our A. baumannii population and their putative role as an alternative mechanism of resistance to third-generation cephalosporins in this species. The bla (CTX-M-2) gene is widespread among the Enterobacteriaceae isolates from our country; however, it was not found in 76 isolates A. baumannii non-epidemiologically related clinical isolates resistant to third-generation cephalosporins ...	2011	22119898
Cerebral Abscess Complicating Proteus mirabilis Meningitis in a Newborn Infant.	Neonatal bacterial meningitis is associated with high morbidity and mortality, especially when complicated by intracranial abscesses. The authors report the case of an 11-day-old female with Proteus mirabilis meningitis complicated by an asymptomatic brain abscess. With this report, the authors illustrate the importance of surveillance neuroradiologic imaging in patients with Proteus meningitis.	2011	22114212
mutually facilitated dispersal between the nonmotile fungus aspergillus fumigatus and the swarming bacterium paenibacillus vortex.	in the heterogeneous environment surrounding plant roots (the rhizosphere), microorganisms both compete and cooperate. here, we show that two very different inhabitants of the rhizosphere, the nonmotile fungus aspergillus fumigatus and the swarming bacterium paenibacillus vortex, can facilitate each other's dispersal. a. fumigatus conidia (nonmotile asexual fungal spores) can be transported by p. vortex swarms over distances of at least 30 cm and at rates of up to 10.8 mm h(-1). moreover, conidi ...	2011	22106274
chromosomal integration and location on inct plasmids of the blactx-m-2 gene in proteus mirabilis clinical isolates.	analysis of five ctx-m-2-producing proteus mirabilis isolates in japan demonstrated that bla(ctx-m-2) was located on the chromosome in four isolates and on inct plasmids in three isolates, including two isolates that also carried the gene on the chromosome. in all four isolates with chromosomal bla(ctx-m-2), isecp1 was responsible for the integration of the gene into the chromosome. three different sites in the p. mirabilis genomic sequence were utilized as integration sites.	2011	22106217
sublethal ciprofloxacin treatment leads to resistance via antioxidant systems in proteus mirabilis.	this study investigates new aspects of the possible role of antioxidant defenses in the mechanisms of resistance to ciprofloxacin in proteus mirabilis. four ciprofloxacin-resistant variants (crvs), selected in vitro by repeated cultures in a sub-minimum inhibitory concentration (mic) concentration of ciprofloxacin, attained different levels of antibiotic resistance and high ferric reducing antioxidant power, with 10(-6) frequencies. however, no mutations occurred in positions 83 or 87 of gyra, ...	2011	22092852
Plasmid-mediated transfer of the bla(NDM-1) gene in Gram-negative rods.	The latest threat of multidrug-resistant Gram-negative bacteria corresponds to the emergence of carbapenemase NDM-1 (New Delhi metallo-ß-lactamase) producers, mostly in Enterobacteriacae. Five bla(NDM) (-1) -positive plasmids of different incompatibility groups (IncL/M, FII, A/C and two untypeable plasmids) from clinical Enterobacteriaceae were evaluated for conjugation properties and host specificity. Successful conjugative transfers were obtained using all tested enterobacterial species as rec ...	2011	22092811
control of clinical pathogens by the haemolymph of paratelphusa hydrodromous, a freshwater crab.	in the present study, effort has been made to find the antimicrobial activity of haemolymph collected from freshwater crab, paratelphusa hydrodromous. the haemolymph collected was tested for antimicrobial assay by disc diffusion method against clinical pathogens. five bacterial species, namely, escherichia coli, klebsiella pneumonia, proteus mirabilis, pseudomonas aeruginosa, staphylococcus aureus, and five fungal strains, namely and aspergillus flavus, aspergillus fumigatus, aspergillus niger, ...	2011	22084719
Perturbation of FliL Interferes with Proteus mirabilis Swarmer Cell Gene Expression and Differentiation.	Proteus mirabilis is a dimorphic, motile bacterium often associated with urinary tract infections. Colonization of urinary tract surfaces is aided by swarmer cell differentiation, which is initiated by inhibition of flagellar rotation when the bacteria first contact a surface. Mutations in fliL, encoding a flagellar structural protein with an enigmatic function, result in the inappropriate production of differentiated swarmer cells, called pseudoswarmer cells, under noninducing conditions, indic ...	2012	22081397
Development of an antibiogram in a long-term care facility.	To create an antibiogram-a profile of an organism's susceptibility/resistance to a panel of antibiotics- for a long-term care facility to assess the prevalence of resistance of bacteria present at the facility.	2011	22079792
proteomic analysis of proteus mirabilis outer membrane proteins reveals differential expression in vivo vs. in vitro conditions.	proteus mirabilis is an opportunistic pathogen that frequently causes complicated urinary tract infections. among a wide spectrum of potential virulence factors, outer membrane proteins (omps) are critical for bacterial interactions and survival in different environments. in this work, we used a proteomic approach to assess p. mirabilis in vivo omps expression compared to in vitro, including iron replete and iron-restricted conditions. three putative iron receptors, irea, pmi0842, and pmi2596, w ...	2011	22077220
biogenic amines produced by enterobacteriaceae isolated from meat products.	biogenic amines in ground meat and processed meat products are one of the indicators to determine the poor quality raw material. major histamine forming bacterium was escherichia coli (strain ec04 with 65.88 mg/100 ml in brain heart infusion medium), followed by the microorganisms morganella morganii (strain mm4 with 8.40 mg/100 ml and strain mm7 with 8.28 mg/100 ml) and proteus mirabilis (strain pm02 with 8.76 mg/100 ml), respectively. the highest putrescine production level was found in citrob ...	2001	22062111
[virulence factors in proteus spp. bacteria isolated from urinary tract infections: their detection and importance].	nosocomial infections associated with biofilm formation have been a serious problem in recent years. up to 32 % of them are urinary tract infections in patients with long-dwelling catheters. catheters represent an ideal surface for bacterial adhesion, facilitating easier colonization of the urinary tract. important pathogens causing these infections are bacteria of the genus proteus that colonize catheters not only by biofilm formation but also using other virulence factors. those were developed ...	2011	22052099
clinical characteristics and outcomes of patients with extended-spectrum β-lactamase-producing bacteremias in the emergency department.	extended-spectrum β-lactamase (esbl)-producing bacteria have been spreading from hospitals to communities. despite this, there are limited emergency department (ed) patient-based studies about these bacteremias. a retrospective matched case-control study with a ratio of 1:3 was conducted at a university hospital. the case group consisted of patients aged >16 years with esbl-producing bacteremias in the ed. patients matched for age and sex with non-esbl-producing bacteremias were sampled as the c ...	2011	22033790
[antimicrobial resistance pattern for gram-negative uropathogens isolated from hospitalised patients and outpatients in cartagena, 2005-2008].	determining the microbial aetiology spectrum and antibiotic resistance pattern of uropathogens causing urinary tract infections in hospitalised patients and outpatients.	2010	22030688
isolation and identification of microbes associated with mobile phones in dammam in eastern saudi arabia.	this study was conducted to determine microbial contamination of mobile phones in the city of dammam, in the eastern region of saudi arabia, and identify the most important microbial species associated with these phones in order to take the necessary remedial measures.	2010	22022665
Molecular mimicry between spermatozoa and bacteria.	Sperm immobilization factor isolated from Staphylococcus aureus immobilizes human spermatozoa as well as motile bacteria, showing that sperm immobilization factor receptor might be shared by bacteria and human spermatozoa. Thus, we sought to identify a common sperm immobilization factor binding receptor on spermatozoa and bacteria.	2011	22019166
Isolation and identification of antibacterial compound from the leaves of Cassia auriculata.	Antimicrobial properties of medicinal plants and plant parts such as flowers, roots, fruits, seeds and oils are being used to cure some chronic and acute diseases throughout the world. In the present study, an attempt has been made to isolate and identify the antibacterial compound present in the leaves of the Cassia auriculata.	2011	22013726
[evaluation of chromogenic medium uriselect4 in urine culture].	we evaluated the performance and the cost of chromogenic medium uriselect4 agar with regard to the standard medium for the detection and identification of urinary tract pathogens. a total of 503 clinical urine specimens containing leucocytes greater or equal to 104/ml were analysed prospectively, in parallel by two different persons on blood agar (gs) and uriselect4 according to the manufacturers' instructions. of the 503 urine specimens tested, 210 gave a positive culture on uriselect4 versus 1 ...	2011	22008133
Pulmonary Zygomycosis with Cunninghamella bertholletiae in a Killer Whale (Orcinus orca).	An adult female killer whale (Orcinus orca) was transported to the Port of Nagoya public aquarium in June 2010. While the animal was being maintained in the aquarium there was a gradual decrease in body weight. On October 1st, 2010 the whale exhibited signs of gastrointestinal disease and died on January 14th, 2011. At necropsy examination the gastric compartments were filled with a large number of variably-sized rocks (total weight 81.4 kg) and there was marked ulceration in the third compartme ...	2011	22000035
serotyping of proteus mirabilis clinical strains based on lipopolysaccharide o-polysaccharide and core oligosaccharide structures.	the aim of this work was to serotype proteus mirabilis urinary tract infection (uti) strains based on chemically defined o-antigens with the use of two clinical collections from sweden and poland consisting of 99 and 24 uti strains, respectively. a simple two-step serotyping scheme was proposed using enzyme immunoassay with heat-stable surface antigens of proteus cells and immunoblotting with isolated lipopolysaccharides (lpss). using polyclonal anti-p. mirabilis rabbit antisera, 50 swedish and ...	2011	21999547
Structure of the core part of the lipopolysaccharide from Proteus mirabilis genomic strain HI4320.	The structure of the core part of the lipopolysaccharide from Proteus mirabilis genomic strain HI4320 was studied. Core oligosaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide and analyzed by NMR spectroscopy and mass spectrometry as well as chemical methods. The structure of the oligosaccharide was established.	2011	21999542
phytochemical constituents and antibacterial efficacy of the flowers of peltophorum pterocarpum (dc.) baker ex heyne.	to investigate the preliminary phytochemistry and antibacterial activity of the flower extract of peltophorum pterocarpum .	2011	21967698
Use of a DNA microarray for detection and identification of bacterial pathogens associated with fishery products.	We established a microarray for the simultaneous detection and identification of diverse putative pathogens often associated with fishery products by targeting specific genes of Listeria monocytogenes, Salmonella, Shigella, Staphylococcus aureus, Streptococcus pyogenes, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, and Yersinia enterocolitica and the 16S-23S rRNA gene internal transcribed spacer (ITS) region of Proteus mirabilis and Proteus vulgaris. The microarray contained 26 sp ...	2011	21965411
Inhibition of escherichia coli and proteus mirabilis adhesion and biofilm formation on medical grade silicone surface.	Silicone has been utilized extensively for biomedical devices due to its excellent biocompatibility and biodurability properties. However, its surface is easily colonized by bacteria which will increase the probability of nosocomial infection. In the present work, a hydrophilic antimicrobial carboxymethyl chitosan (CMCS) layer has been grafted on medical grade silicone surface pre-treated with polydopamine (PDA). The increase in hydrophilicity was confirmed from contact angle measurement. Bacter ...	2012	21956834
development of an "early warning" sensor for encrustation of urinary catheters following proteus infection.	biofilm formation in long-term urinary catheterized patients can lead to encrustation and blockage of urinary catheters with serious clinical complication. catheter encrustation stems from infection with urease-producing bacteria, particularly proteus mirabilis. urease generates ammonia from urea, and the elevated ph of the urine results in crystallization of calcium and magnesium phosphates, which block the flow of urine. the aim of this research is to develop an "early warning" silicone sensor ...	2012	21954120
Effective cohorting and "superisolation" in a single intensive care unit in response to an outbreak of diverse multi-drug-resistant organisms.	Cohorting patients in dedicated hospital wards or wings during infection outbreaks reduces transmission of organisms, yet frequently, this may not be feasible because of inadequate capacity, especially in the intensive care unit (ICU). We hypothesized that cohorting isolation patients in one geographic location in a single ICU and using enhanced isolation procedures ("superisolation") can prevent the further spread of highly multi-drug-resistant organisms (MDRO).	2011	21936667
proteus mirabilis biofilms and catheter-associated urinary tract infections.	proteus mirabilis inhabits the environment and causes a number of infections including those of the skin, respiratory tract, wounds and urinary tract. these organisms express virulence factors associated with adhesion, motility, immunoavoidance, nutrient acquisition, host damage, as well as biofilm formation. p. mirabilis produces biofilms in diverse habitats with those formed in the human host playing a key role in indwelling device infections. the most studied p. mirabilis biofilms are those f ...	2011	21921687
urinary pathogens and drug susceptibility patterns of urinary tract infections among antenatal clinic attendees in ibadan, nigeria.	aim: to determine the bacterial agents involved in urinary tract infections in pregnant women and their antibiotic susceptibility patterns in ibadan, nigeria. methods: all consenting subjects who attended antenatal clinics of the university college hospital and adeoyo maternity hospital, ibadan, nigeria, from 1 april 2007 and 30 march 2009 were interviewed to obtain demographic and pregnancy health data. mid-stream urine samples obtained were processed by standard methods. confirmed bacterial ...	2011	21917070
vim and imp metallo-β-lactamases and other extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae from environmental samples in a tunisian hospital.	an extremely drug-resistant enterobacteriaceae species emerged in kasserine hospital, tunisia between 2009 and 2010 causing a local outbreak. we aimed to characterize extended-spectrum β-lactamase (esbl) and metallo-β-lactamase (mbl)-producing enterobacteriaceae from the hospital environment. swabs were collected from ten different wards from kasserine hospital, tunisia. a total of 46 isolates were cultured onto macconkey agar supplemented with ceftazidime to select for esbl-producing enterobact ...	2011	21917010
[Uncomplicated lower urinary tract infections in females--clinical aspects, aetiology and antimicrobial resistance epidemiology. Results of the ARESC (Antimicrobial Resistance Epidemiological Survey on Cystitis) study in Poland and their implications for empiric therapy].	The Polish results of the international ARESC study are reported concerning clinical aspects, epidemiology, and antimicrobial susceptibility of uropathogens.	2011	21913487
Impact of adopting minimum inhibitory concentration as the determinant of susceptibility to cephalosporins and carbapenems in multi-drug resistant Enterobacteriaceae.	The Clinical and Laboratory Standards Institute has recommended that Enterobacteriaceae susceptibility to most cephalosporins and carbapenems be reported according to minimum inhibitory concentration (MIC) alone. We analyzed our record of multi-drug resistant Enterobacteriaceae to assess the impact of these changes. We compared susceptibilities of ceftriaxone-resistant Enterobacteriaceae when using the 2009 and new 2010 MIC standards. Vitek2® (BioMerieux), was used to assess the changes in susce ...	2011	21912922
in vitro susceptibility of nosocomial isolates against a new aminoglycoside isepamicin.	isepamicin is a new aminoglycoside with a spectrum of activity similar to amikacin with an advantage of possessing a high level of stability against different aminoglycoside modifying enzymes. the in vitro susceptibility of nosocomial isolates obtained from patients admitted to the all india institute of medical sciences, new delhi during october-december, 1999 was tested against isepamicin and compared with other aminoglycosides.	2001	21910285
antibiotic sensitivity profile of bacterial pathogens in postoperative wound infections at a tertiary care hospital in gujarat, india.	to find out the most common bacterial pathogens responsible for post-operative wound infection and their antibiotic sensitivity profile.	2011	21897707
photoletter to the editor: oral ulceration in pyoderma gangrenosum.	a 65-year-old woman presented with widespread necrotising cutaneous ulceration and oral involvement. past history included rheumatoid arthritis, and a left nephrectomy.examination revealed multiple violaceous undermined ulcers. blood investigations showed an acute inflammatory response. skin histopathology showed epidermal ulceration with acute and chronic inflammation. direct immunofluorescence was negative. a diagnosis of pyoderma gangrenosum with oral involvement was made. mycophenolate mofet ...	2011	21894254
outbreak caused by proteus mirabilis isolates producing weakly expressed tem-derived extended-spectrum β-lactamase in spinal cord injury patients with recurrent bacteriuria.	we performed a retrospective extended-spectrum β-lactamase (esbl) molecular characterization of proteus mirabilis isolates recovered from urine of spinal cord injury patients. a incorrectly detected tem-24-producing clone and a new weakly expressed tem-derived esbl were discovered. in such patients, esbl detection in daily practice should be improved by systematic use of a synergy test in strains of p. mirabilis resistant to penicillins.	2011	21888562
phenolic compounds and biological activity of kitaibelia vitifolia.	this study was aimed at evaluating the antioxidant activity and efficacy of the ethanolic extract of the endemic plant species kitaibelia vitifolia in inhibiting the growth of selected fungi and bacteria. antimicrobial activity was tested using the broth dilution procedure for determination of minimum inhibitory concentration (mic). mics were determined for eight selected indicator strains. the highest susceptibility to k. vitifolia ethanolic extract among the bacteria tested was exhibited by ba ...	2011	21877950
antimicrobial and toxicological activities of five medicinal plant species from cameroon traditional medicine.	infectious diseases caused by multiresistant microbial strains are on the increase. fighting these diseases with natural products may be more efficacious. the aim of this study was to investigate the in vitro antimicrobial activity of methanolic, ethylacetate (etoac) and hexanic fractions of five cameroonian medicinal plants (piptadeniastum africana, cissus aralioides, hileria latifolia, phyllanthus muellerianus and gladiolus gregasius) against 10 pathogenic microorganisms of the urogenital and ...	2011	21867554
development of 3d architecture of uropathogenic proteus mirabilis batch culture biofilms-a quantitative confocal microscopy approach.	this work studies the development of the 3d architecture of batch culture p. mirabilis biofilms on the basis of morpho-topological descriptors calculated from confocal laser scanning microscopy (clsm) stacks with image processing routines. a precise architectonical understanding of biofilm organization on a morpho-topological level is necessary to understand emergent interactions with the environment and the appearance of functionally different progeny swarmer cells. p. mirabilis biofilms were g ...	2011	21864585
nosocomial infection and characterization of extended-spectrum β-lactamases-producing enterobacteriaceae in northeast brazil.	extended spectrum β-lactamases (esbls) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide.	2011	21860889
genetic features of blandm-1-positive enterobacteriaceae.	genetic features associated with the bla(ndm-1) gene were investigated in 6 escherichia coli, 7 klebsiella pneumoniae, 1 citrobacter freundii, 1 proteus mirabilis, and 1 providencia stuartii isolate of worldwide origin. clonal diversity was observed for both e. coli and k. pneumoniae. the bla(ndm-1) gene was carried by different plasmid types (inca/c, incf, incl/m, or untypeable) and was likely chromosome borne in two isolates. the bla(ndm-1) plasmids coharbored a variety of resistance determina ...	2011	21859933
dissemination of the rmtb gene carried on incf and incn plasmids among enterobacteriaceae in a pig farm and its environment.	to investigate the prevalence and characterization of 16s rrna methylase-producing bacteria in a pig farm and its environment in east china.	2011	21852287
the new variant of salmonella genomic island 1 (sgi1-v) from a proteus mirabilis french clinical isolate harbours blaveb-6 and qnra1 in the multiple antibiotic resistance region.	the clinical strain of proteus mirabilis vb1248 isolated from a blood culture in august 2009 was multiresistant (i.e. resistant to β-lactams, fluoroquinolones, aminoglycosides and sulphonamides). we searched for the presence of a salmonella genomic island 1 (sgi1).	2011	21846670
investigation of the antimicrobial activity of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.).	the aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. material and methods. ethanol extract from the leaves of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.) was produced at the department of food technology, kaunas university of technology. the antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria s ...	2011	21822040
[russian results of the international epidemiological study of clinical aspects and resistance to antimicrobial medicines of cystitis uropathogens in females (aresc): significance of empiric therapy].	a total of 656 female patients from russia aged between 18 to 65 years with uncomplicated cystitis entered the international aresc trial, of them 647 were eligible for final analysis. positive cultural urine tests ( > 10(4) cfu/ml) were in 419 (64.7%) patients, 393 (93.8%) patients had monoinfection. the central laboratory of genoa (to which the isolated samples were sent) has tested 416 uropathogens from 399 patients. the following pathogens were isolated: e. coli (72.6%), enterococcus (7.0%), ...	2011	21815455
[urinary tract infection in patient after brain injury].	intruduction: one of the most important problems in treating patients after head injury is urinary complication. the first place take urinary infections, which disturb rehabilitation process. clean intermittent catherization, bladder training and good care do not preserve infection. the purpose of our study was to present the frequency of urinary infections and main pathogens involved in these infections among patients treated in the department of rehabilitation after head injury.	2011	21812229
effect of curcumin against proteus mirabilis during crystallization of struvite from artificial urine.	we investigated the activity of curcumin against proteus mirabilis and the struvite crystallization in relation to urinary stones formation. in order to evaluate an activity of curcumin we performed an in vitro experiment of struvite growth from artificial urine. the crystallization process was induced by proteus mirabilis to mimic the real urinary tract infection, which usually leads to urinary stone formation. the results demonstrate that curcumin exhibits the effect against proteus mirabilis ...	2012	21808656
new delhi metallo-ss-lactamase-1: detection and prevention.		2011	21788418
[clinical and economic expedience of ertapenem therapy of complicated urinary tract infection].	clinical and economic investigation of various antibiotics use in the treatment of complicated urinary tract infection (cuti) was performed under the russian economic environment. the drugs of comparison were ertapenem, ceftriaxone and levofloxacin. direct costs and their structure were shown, and the cost efficiency was calculated. alternative analysis and one-side susceptibility analysis were performed. in complicated urinary tract infections when the major pathogens were escherichia coli, kle ...	2011	21780670
[proteus mirabilis isolated from urine, resistance to antibiotics and biofilm formation].	in the rather rare urinary pathogen proteus mirabilis resistance to antibiotics and ability to form biofilm were studied.	2011	21780025
antibacterial activity of selected ethnomedicinal plants from south india.	to screen the antimicrobial potential of three ethnomedicinal plants chassalia curviflora thw. (c. curviflora), cyclea peltata hook. f. & thomson (c. peltata) and euphorbia hirta l (e. hirta) used in folk medicines in aarukani hills kani tribe, tamil nadu, india against human bacterial pathogens.	2011	21771680
comprehensive inhibitor profiling of the proteus mirabilis metalloprotease virulence factor zapa (mirabilysin).	in this study we report for the first time the comprehensive inhibitor profiling of the proteus mirabilis metalloprotease virulence factor zapa (mirabilysin) using a 160 compound focused library of n-alpha mercaptoamide dipeptides, in order to map the s(1)(') and s(2)(') binding site preferences of this important enzyme. this study has revealed a preference for the aromatic residues tyrosine and tryptophan in p(1)(') and aliphatic residues in p(2)('). from this library, six compounds were identi ...	2011	21762758
susceptibility of esbl-producing enterobacteriaceae with the new clsi breakpoints.	in 2010 clsi lowered the susceptibility breakpoints of some cephalosporins and aztreonam for enterobacteriaceae and eliminated the need to perform esbl screening and confirmatory tests. the aim of this study was to determine how many esbl-producing strains will test susceptible for three common species of enterobacteriaceae using the new breakpoints. as determined with the clsi screening and confirmatory tests, 382 consecutive esbl-producing strains were collected at our hospital between 2007 an ...	2011	21752977
prevalence of sxt/r391-like integrative and conjugative elements carrying blacmy-2 in proteus mirabilis.	objectives to characterize the vectors involved in the dissemination of bla(cmy-2) genes in clinical isolates of proteus mirabilis collected between 1999 and 2007. methods plasmid analysis of 19 p. mirabilis carrying ampc genes was performed by pcr-based replicon typing, s1-pfge and southern hybridization with ampc and replicon probes. isolates that could not be characterized were examined for the presence of sxt/r391-like elements. to demonstrate the involvement of these elements in the dissemi ...	2011	21752830
antimicrobial activity of two essential oils.	the aim of the study was to evaluate the antimicrobial activity of essential oils in vitro for possible application to reduce the content of microorganisms in the air of animal houses. the essential oils of cymbopogon citrarus l. and malaleuca alternifolia l. were screened against bacteria staphylococcus aureus, enterococcus faecium, pseudomonas aeruginosa, escherichia coli, proteus mirabilis and yeast candida albicans. the minimal inhibitory concentration of the active essential oils was tested ...	2011	21739934
purple urine.		2011	21738253
microbiological profile with antibiotic sensitivity pattern of cholesteatomatous chronic suppurative otitis media among children.	chronic suppurative otitis media (csom) is the most common cause of childhood hearing impairment in the developing countries and atticoantral type is associated with increased incidence of intracranial and extracranial complications. this study was undertaken to define the microbiology of atticoantral type of chronic otitis media and the antibiotic sensitivity pattern, thereby reducing the potential risks of complications.	2011	21715027
acidic environments induce differentiation of proteus mirabilis into swarmer morphotypes.	although swarmer morphotypes of proteus mirabilis have long been considered to result from surfaced-induced differentiation, the present findings show that, in broth medium containing urea, acidic conditions transform some swimmer cells into elongated swarmer cells. this study has also demonstrates that p. mirabilis cells grown in acidic broth medium containing urea enhance virulence factors such as flagella production and cytotoxicity to human bladder carcinoma cell line t24, though no signific ...	2011	21707738
studies of antimicrobial activities of some 4-thiazolidinone fused pyrimidines, [1,5]-benzodiazepines and their oxygen substituted hydroxylamine derivatives.	thiazolidin-4-one fused pyrimidines, [1,5]-benzodiazepines and their oxygen substituted hydroxylamine derivatives have been screened for antibacterial, antifungal and antimalarial activity. bacillus subtilis, escherichia coli, proteus mirabilis and salmonella typhi were used for antibacterial screening. aspergillus fumigatus and candida albicans were used for antifungal screening and plasmodium species were used for antimalarial screening. the antibacterial and antifungal activities are expresse ...	2010	21694993
anti-proliferative activity of meroditerpenoids isolated from the brown alga stypopodium flabelliforme against several cancer cell lines.	the sea constitutes one of the most promising sources of novel compounds with potential application in human therapeutics. in particular, algae have proved to be an interesting source of new bioactive compounds. in this work, six meroditerpenoids (epitaondiol, epitaondiol diacetate, epitaondiol monoacetate, stypotriol triacetate, 14-ketostypodiol diacetate and stypodiol) isolated from the brown alga stypopodium flabelliforme were tested for their cell proliferation inhibitory activity in five ce ...	2011	21673894
self-transmissibility of the integrative and conjugative element icepm1 between clinical isolates requires a functional integrase, relaxase, and type iv secretion system.	integrative and conjugative elements (ices), which are chromosomal mobile elements, can conjugatively transfer between bacteria. recently, we identified a genomic island of proteus mirabilis, a common agent of catheter-associated urinary tract infection (uti), that possesses all the properties consistent with an ice. this element, designated icepm1, is highly conserved in other causative agents of uti, suggesting its mobility. we demonstrate that icepm1 can actively excise from the chromosome in ...	2011	21665966
jacaranda cuspidifolia mart. (bignoniaceae) as antibacterial agent.	abstract this study evaluated, in vitro, the antimicrobial activity of the hexane extract (jche), methanol extract (jcme), and chloroform fraction (jccf) of bark from jacaranda cuspidifolia mart. (family bignoniaceae), a brazilian medicinal plant, traditionally used as anti-syphilis and anti-gonorrhea treatment. the antimicrobial activity was evaluated using the disc diffusion method followed by the determination of minimum inhibitory concentration (mic) values. jche was not active against the b ...	2011	21663482