Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| transformation of a methylotrophic bacterium, methylobacterium extorquens, with a broad-host-range plasmid by electroporation. | electroporation was used to transform the methylotrophic bacterium methylobacterium extorquens with broad-host-range plasmid pla2917, which contains a gene specifying resistance to kanamycin. plasmid dna was introduced into m. extorquens in the presence of an electric pulse, and kanamycin-resistant transformants were obtained. these transformants harbored plasmid dna that was identical to plasmid pla2917. we examined several factors independently and found up to 8 x 10(3) transformants per micro ... | 1991 | 1809210 |
| nucleotide sequence of the amicyanin gene from methylobacterium extorquens am1. | the gene for amicyanin from the methylotrophic bacterium, methylobacterium extorquens am1 was identified. it encodes a protein consisting of 119 amino acids with a molecular weight of 12,609 kda. the amino acid sequence shows the presence of a typical leader sequence and signal peptidase recognition site. two putative hairpin structures were found, one located directly behind the amicyanin gene and another located 50 bp upstream. the same sequence aaaatccc was found near the start codons for the ... | 1991 | 1802036 |
| cloning, mutagenesis, and physiological effect of a hydroxypyruvate reductase gene from methylobacterium extorquens am1. | the gene encoding the serine cycle hydroxypyruvate reductase of methylobacterium extorquens am1 was isolated by using a synthetic oligonucleotide with a sequence based on a known n-terminal amino acid sequence. the cloned gene was inactivated by insertion of a kanamycin resistance gene, and recombination of this insertion derivative with the wild-type gene produced a serine cycle hydroxypyruvate reductase null mutant. this mutant had lost its ability to grow on c-1 compounds but retained the abi ... | 1992 | 1729225 |
| 16s ribosomal rna sequence analysis for determination of phylogenetic relationship among methylotrophs. | 16s ribosomal rnas (rrna) of 12 methylotrophic bacteria have been almost completely sequenced to establish their phylogenetic relationships. methylotrophs that are physiologically related are phylogenetically diverse and are scattered among the purple eubacteria (class proteobacteria). group i methylotrophs can be classified in the beta- and the gamma-subdivisions and group ii methylotrophs in the alpha-subdivision of the purple eubacteria, respectively. pink-pigmented facultative and non-pigmen ... | 1990 | 1693657 |
| purification and characterization of hydroxypyruvate reductase from the facultative methylotroph methylobacterium extorquens am1. | hydroxypyruvate reductase was purified to homogeneity from the facultative methylotroph methylobacterium extorquens am1. it has a molecular mass of about 71 kda, and it consists of two identical subunits with a molecular mass of about 37 kda. this enzyme uses both nadh (km = 0.04 mm) and nadph (km = 0.06 mm) as cofactors, uses hydroxypyruvate (km = 0.1 mm) and glyoxylate (km = 1.5 mm) as the only substrates for the forward reaction, and carries out the reverse reaction with glycerate (km = 2.6 m ... | 1991 | 1657886 |
| isolation, sequencing, and mutagenesis of the gene encoding cytochrome c553i of paracoccus denitrificans and characterization of the mutant strain. | the periplasmically located cytochrome c553i of paracoccus denitrificans was purified from cells grown aerobically on choline as the carbon source. the purified protein was digested with trypsin to obtain several protein fragments. the n-terminal regions of these fragments were sequenced. on the basis of one of these sequences, a mix of 17-mer oligonucleotides was synthesized. by using this mix as a probe, the structural gene encoding cytochrome c553i (cycb) was isolated. the nucleotide sequence ... | 1991 | 1657873 |
| isolation and characterization of the moxj, moxg, moxi, and moxr genes of paracoccus denitrificans: inactivation of moxj, moxg, and moxr and the resultant effect on methylotrophic growth. | by using the moxf gene encoding the large fragment of methanol dehydrogenase as a probe, a downstream linked chromosomal fragment was isolated from a genomic bank of paracoccus denitrificans. the nucleotide sequence of the fragment was determined and revealed the 3' part of moxf, four additional open reading frames, and the 5' part of a sixth one. the organization and deduced amino acid sequences of the first three frames downstream from moxf were found to be largely homologous to the moxj, moxg ... | 1991 | 1657871 |
| genetic organization of methylamine utilization genes from methylobacterium extorquens am1. | an isolated 5.2-kb fragment of methylobacterium extorquens am1 dna was found to contain a gene cluster involved in methylamine utilization. analysis of polypeptides synthesized in an escherichia coli t7 expression system showed that five genes were present. two of the genes encoded the large and small subunits of methylamine dehydrogenase, and a third encoded amicyanin, the presumed electron acceptor for methylamine dehydrogenase, but the function of the other two genes is not known. the order o ... | 1991 | 1653226 |
| cloning of a methanol-inducible moxf promoter and its analysis in moxb mutants of methylobacterium extorquens am1rif. | in methylobacterium extorquens am1, gene encoding methanol dehydrogenase polypeptides are transcriptionally regulated in response to c1 compounds, including methanol (m. e. lidstrom and d. i. stirling, annu. rev. microbiol. 44:27-57, 1990). in order to study this regulation, a transcriptional fusion has been constructed between a beta-galactosidase reporter gene and a 1.55-kb xhoi-sali fragment of m. extorquens am1rif dna encoding the n terminus of the methanol dehydrogenase large subunit (moxf) ... | 1992 | 1624436 |
| catheter infection caused by methylobacterium in immunocompromised hosts: report of three cases and review of the literature. | three cases of catheter infection due to methylobacterium extorquens are reported. each patient had a history of acute leukemia and was immunocompromised; two had undergone bone marrow transplantation, and the third was receiving consolidation chemotherapy. all three patients survived after removal of the central venous catheter and antibiotic treatment. the clinical features of these cases are compared with those of the 12 previously reported cases of infection due to methylobacterium species. | 1992 | 1600002 |
| molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria. | the current knowledge on the structure and on the organization of polyhydroxyalkanoic acid (pha)-biosynthetic genes from a wide range of different bacteria, which rely on different pathways for biosynthesis of this storage polyesters, is provided. molecular data will be shown for genes of alcaligenes eutrophus, purple non-sulfur bacteria, such as rhodospirillum rubrum, purple sulfur bacteria, such as chromatium vinosum, pseudomonads belonging to rrna homology group i, such as pseudomonas aerugin ... | 1992 | 1476773 |
| crystallization and preliminary crystallographic investigation of methanol dehydrogenase from methylobacterium extorquens am1. | single crystals of methanol dehydrogenase (mdh) from methylobacterium extorquens am1 have been grown by the vapour diffusion method. these crystals diffract to beyond 2 a resolution and are suitable for x-ray crystallography. they belong to the orthorhombic space group p2(1)2(1)2(1) and have the following unit cell parameters: a = 66.79 a, b = 108.9 a, c = 188.9 a. one asymmetric unit contains an alpha 2 beta 2 tetramer of mdh and the location of the non-crystallographic 2-fold symmetry axis of ... | 1992 | 1447790 |
| characterization of mutant forms of the quinoprotein methanol dehydrogenase lacking an essential calcium ion. | methanol dehydrogenase (mdh) from methylobacterium extorquens, methylophilus methylotrophus, paracoccus denitrificans and hyphomicrobium x all contained a single atom of ca2+ per alpha 2 beta 2 tetramer. the role of ca2+ was investigated using the mdh from methylobacterium extorquens. this was shown to be similar to the mdh from hyphomicrobium x in having 2 mol of prosthetic group (pyrroloquinoline quinine; pqq) per mol of tetramer, the pqq being predominantly in the semiquinone form. mdh isolat ... | 1992 | 1332681 |
| the three-dimensional structures of methanol dehydrogenase from two methylotrophic bacteria at 2.6-a resolution. | the structures of methanol dehydrogenase (medh) from two closely related methylotrophic bacteria, methylophilus methylotrophus and w3a1, have been determined at 2.6-a resolution. the molecule, a quinoprotein of molecular mass of about 138 kda, contains two heavy (h) and two light (l) subunits of unknown sequence and two molecules of noncovalently associated pyrroloquinoline quinone. the two enzymes crystallize isomorphously in space group p2(1) with one h2l2 heterotetramer in the asymmetric unit ... | 1992 | 1331050 |
| the interaction of methanol dehydrogenase and its electron acceptor, cytochrome cl in methylotrophic bacteria. | the interactions of methanol dehydrogenase (mdh, ec1.1.99.8) with its specific electron acceptor cytochrome cl has been investigated in methylobacterium extorquens and methylophilus methylotrophus. the mdhs of these two very different methylotrophs have the same alpha 2 beta 2 structure; the interaction of these mdhs with their specific electron acceptor, cytochrome cl, has been studied using a novel assay system. electrostatic reactions are involved in 'docking' of the two proteins. edta inhibi ... | 1992 | 1311606 |
| the microbial metabolism of c1 compounds. the electron-transport chain of pseudomonas am1. | pseudomonas am1, hyphomicrobium x and pseudomonas ms all contain cytochrome a/a(3) and a b-type cytochrome able to react with co. pseudomonas am1 and hyphomicrobium x also have a co-binding cytochrome c. the purified cytochrome c (redox potential 0.26v) of pseudomonas am1 was not susceptible to oxidation by molecular oxygen. co reacted slowly with the reduced form giving a co difference spectrum with a peak at 412nm and troughs at 420nm and 550nm. similar results were obtained with the cytochrom ... | 1975 | 1220689 |
| bacterial flora of the schistosome vector snail biomphalaria glabrata. | the aerobic heterotrophic bacterial flora in over 200 individuals from 10 wild populations and 3 laboratory colonies of the schistosome vector snail biomphalaria glabrata was examined. internal bacterial densities were inversely proportional to snail size and were higher in stressed and laboratory-reared snails. the numerically predominant bacterial genera in individual snails included pseudomonas, acinetobacter, aeromonas, vibrio, and several members of the enterobacteriaceae. enterobacteriacea ... | 1979 | 539821 |
| fructose metabolism in four pseudomonas species. | 1. atp-dependent phosphorylation of fructose could not be detected in extracts of fructose-grown cells of pseudomonas extorquens strain 16, pseudomonas 3a2, pseudomonas acidovorans and pseudomonas fluorescens. instead, phosphorylation of fructose to fructose-1-phosphate was found to occur when cell-free extracts were incubated with fructose and phosphoenolpyruvate. such an activity could not be detected in cell-free extracts of succinate-grown cells. 2. high levels of 1-phosphofructokinase were ... | 1977 | 143919 |
| purification and properties of the methane mono-oxygenase enzyme system from methylosinus trichosporium ob3b. | 1. a three-component enzyme system that catalyses the oxidation of methane to methanol has been highly purified from methylosinus trichosporium. 2. the components are (i) a soluble co-binding cytochrome c, (ii) a copper-containing protein and (iii) a small protein; the mol. wts. are 13 000, 47 000 and 9400 respectively. the cytochrome component cannot be replaced by similar cytochrome purified from pseudomonas extorquens or by horse heart cytochrome c. 3. the stoicheiometry suggests a mono-oxyge ... | 1977 | 15544 |