Publications

resistance to hygromycin b : a new marker for plant transformation studies.	a bacterial gene encoding hygromycin phosphotransferase has been modified for expression in tobacco cells. the aphiv gene from escherichia coli was inserted between the 5' sequence of an octopine synthase gene and the 3' sequence from a nopaline synthase gene. the new gene was incorporated between t-dna border fragments in the broad-host-range vector pkt210 to form a micro-ti plasmid. agrobacterium tumefaciens containing this plasmid and a ti plasmid as helper was used to incite crown gall tumor ...	1985	24306569
chromosomal nodulation genes: sym-plasmid containing agrobacterium strains need chromosomal virulence genes (chva and chvb) for nodulation.	the chromosomal genes chva and chvb of agrobacterium tumefaciens, which mediate attachment to plant cells, were found to be essential not only for tumour induction but also for the formation of root nodules on plants.	1987	24302529
functional analysis of the agrobacterium tumefaciens octopine ti-plasmid left and right t-region border fragments.	border fragments of the octopine ti-plasmid were tested for their ability to restore tumorigenicity of an avirulent mutant carrying a deleted right border. it was found that neither introduction of left border fragments nor that of small right border fragments at the position of the deletion resulted in a complete restoration of oncogenicity. however, insertion of a larger right border fragment in the deletion mutant gave fully oncogenic strains. in the latter case sequences to the right side of ...	1987	24302528
conservation of is66 homologue of octopine ti plasmid dna in rhizobium fredii plasmid dna.	dna sequences homologous to the t-dna region of the octopine ti plasmid from agrobacterium tumefaciens are found in various fast-growing rhizobium fredii strains. the largest fragment (bamhi fragment 2) at the right-boundary region of the 'core' t-dna hybridizes to more than one plasmid present in r. fredii. however, one smaller fragment (ecori fragment 19a) adjacent to the 'core' t-dna shows homology only with the plasmid carrying the symbiotic nitrogen-fixation genes (psym). hybridization data ...	1986	24302303
bleomycin resistance: a new dominant selectable marker for plant cell transformation.	plant cells are sensitive to the antibiotic bleomycin, a dna damaging glycopeptide. a bleomycin resistance determinant, located on transposon tn5 and functional in bacteria, has been cloned in a plant expression vector and introduced into nicotiana plumbaginifolia using agrobacterium tumefaciens. the expression of this determinant in plant cells confers resistance to bleomycin and allows selection of transformed plant cells.	1986	24302302
the transformation of zea mays seedlings with agrobacterium tumefaciens.	virulent strains of the soil bacterium agrobacterium tumefaciens infect dicotyledonous plants and elicit a profound neoplastic response which results in crown gall formation (18). the inciting agent has been shown to be a high molecular weight plasmid (ti) a section of which, the t-dna, integrates into the host plant's genome (4, 28, 30). although transformation of this kind was presumed to be limited to dicots, the detection of enzyme activities linked to the expression of t-dna has been demons ...	1986	24302156
segregation of genes transferred to one plant cell from two separate agrobacterium strains.	agrobacterium tumefaciens and agrobacterium rhizogenes are soil bacteria which transfer dna (t-dna) to plant cells. two agrobacterium strains, each with a different t-dna, can infect plants and give rise to transformed tissue which has markers from both t-dnas. although marker genes from both t-dnas are in the tissue, definitive proof that the tissue is a cellular clone and that both t-dnas are in a single cell is necessary to demonstrate cotransformation. we have transferred two distinguishable ...	1987	24301306
acetosyringone promotes high efficiency transformation of arabidopsis thaliana explants by agrobacterium tumefaciens.	high frequency transformation of arabidopsis thaliana leaf explants has been obtained using a disarmed ti plasmid containing the coding region of a neomycin phosphotransferase gene (npt ii) as a selectable marker. the rate of transformation ranged from 55 to 63 percent when acetosyringone (as), a natural wound response molecule, was added to an agrobacterium tumefaciens culture prior to incubation with leaf segments. without acetosyringone, the transformation rate was approximately 2 to 3 percen ...	1987	24301191
direct visualization of agrobacterium-delivered vire2 in recipient cells.	agrobacterium tumefaciens is a natural genetic engineer widely used to deliver dna into various recipients, including plant, yeast and fungal cells. the bacterium can transfer single-stranded dna molecules (t-dnas) and bacterial virulence proteins, including vire2. however, neither the dna nor the protein molecules have ever been directly visualized after the delivery. in this report, we adopted a split-gfp approach: the small gfp fragment (gfp11) was inserted into vire2 at a permissive site to ...	2014	24299048
genome engineering of agrobacterium tumefaciens using the lambda red recombination system.	agrobacterium tumefaciens has been widely used as a tool for transgenesis in plants. the availability of its genome sequence should facilitate the directed engineering of improved properties; however, the current genome engineering options are laborious. here, we investigated whether the lambda red operon can be applied for recombineering of the a. tumefaciens genome. first, we built an expression plasmid for a. tumefaciens employing a tetracycline-inducible promoter to regulate the red operon. ...	2014	24297480
detection of quorum sensing signal molecules in edwardsiella ictaluri ei-151.	edwardsiella ictaluri is a gram-negative pathogenic bacterium in the family enterobacteriaceae that causes enteric septicemia of catfish, which has become a significant problem in the aquaculture of striped catfish (pangasianodon hypophthalmus) in vietnam. in this study, a bacterium designated as ei-151 was isolated from diseased striped catfish and proved to be virulent. based on 16s rdna sequencing and phenotypic tests, the pathogenic bacterium was identified as edw. ictaluri. the presence of ...	2012	24293714
global analysis of differentially expressed genes and proteins in the wheat callus infected by agrobacterium tumefaciens.	agrobacterium-mediated plant transformation is an extremely complex and evolved process involving genetic determinants of both the bacteria and the host plant cells. however, the mechanism of the determinants remains obscure, especially in some cereal crops such as wheat, which is recalcitrant for agrobacterium-mediated transformation. in this study, differentially expressed genes (degs) and differentially expressed proteins (deps) were analyzed in wheat callus cells co-cultured with agrobacteri ...	2013	24278131
the 5' flanking region of a barley b hordein gene controls tissue and developmental specific cat expression in tobacco plants.	the 549 base pairs of the 5' flanking region of a barley seed storage protein (b1 hordein) gene were linked to the reporter gene encoding chloramphenicol acetyl transferase (cat). the chimaeric gene was transferred into tobacco plants using agrobacterium tumefaciens. cat enzyme activity was detected in the seeds, but not in the leaves, of the transgenic plants. furthermore, enzyme activity was found only in the endosperm, and only from fifteen days after pollination. in contrast, the constitutiv ...	1988	24277567
tissue-specific expression of a pea legumin gene in seeds of nicotiana plumbaginifolia.	a 3.4-kilobase genomic dna fragment from pisum sativum l. containing the lega gene, which encodes a major legumin storage protein, was transferred to nicotiana plumbaginifolia using an agrobacterium tumefaciens strain containing the bin 19 binary vector system. northern hybridisation analysis of lega-transformed plants demonstrated that legumin-specific rna was present in developing seeds but not in developing leaves. legumin protein was immunologically detected in the mature seeds of lega-trans ...	1988	24277514
transformation of cotton (gossypium hirsutum l.) by agrobacterium tumefaciens and regeneration of transgenic plants.	cotton (gossypium hirsutum l.) cotyledon tissues have been efficiently transformed and plants have been regenerated. cotyledon pieces from 12-day-old aseptically germinated seedlings were inoculated with agrobacterium tumefaciens strains containing avirulent ti (tumor-inducing) plasmids with a chimeric gene encoding kanamycin resistance. after three days cocultivation, the cotyledon pieces were placed on a callus initiation medium containing kanamycin for selection. high frequencies of transform ...	1987	24277496
molecular characterization of the virulence gene vira of the agrobacterium tumefaciens octopine ti plasmid.	the virulence loci play an essential role in tumor formation by agrobacterium tumefaciens. induction of vir gene expression by plant signal molecules is solely dependent on the virulence loci vira and virg. this study focused on the vira locus of the octopine type ti plasmid pti15955. the nucleic acid sequence of a 5.7-kilobase fragment encompassing vira was determined. genetic analysis of this region revealed that vira contains one open reading frame coding for a protein of 91 639 daltons. immu ...	1987	24277199
assessment of the efficiency of cotransformation of the t-dna of disarmed binary vectors derived from agrobacterium tumefaciens and the t-dna of a. rhizogenes.	co-transfer of agrobacterium rhizogenes t-dna and t-dna from the a. tumefaciens binary vector pbin19 (bevan, 1984) was studied in detail using nicotiana rustica. high frequencies of co-transfer of t-dna's were observed, even when no selection pressure was exerted. increased levels of pbin19 t-dna were found in hairy root cultures with selection at higher levels of kanamycin sulphate (50-200 μg ml(-1)). several other species were also transformed by a. rhizogenes carrying pbin19 and a. rhizogenes ...	1987	24277194
expression of a soybean β-conclycinin gene under the control of the cauliflower mosaic virus 35s and 19s promoters in transformed petunia tissues.	a gene encoding the α'-subunit of β-conglycinin was ligated to the 19s and 35s promoters of cauliflower mosaic virus and introduced into petunia plants on a disarmed ti-plasmid using agrobacterium tumefaciens. transformed cells were regenerated into whole plants and ummunoreactive polypeptides and hybridizable, polyadenylated mrna were detected in transformed tissues. expression from the 35s promoter was 10 to 50 times greater than expression from the 19s promoter. the level of immunodetectable ...	1987	24277084
phytohormones in the formation of crown gall tumors.	crown gall tumors were initiated in a variety of plant species by infection with agrobacterium tumefaciens strain b6 and the concomitant changes in the tissue levels of phytohormones, mainly indole-3-acetic acid (iaa) and cytokinins, were analyzed. a comparison was made of these hormones with those produced by virulent and avirulent strains of the bacterium in liquid culture and with those of bacteria-free crown gall callus cultures. specific radioimmunoassays were employed for hormone determina ...	1981	24276937
transformation of soybean protoplasts from permanent suspension cultures by cocultivation with cells of agrobacterium tumefaciens.	cell wall regenerating protoplasts from soybean cells kept in suspension culture were cocultivated with bacteria which were derived from the nopaline strain c58 of agrobacterium tumefaciens. when the bacteria carried an oncogenic ti-plasmid, about 5% of the surviving protoplasts were able to form calli on hormone-free agar in contrast to controls, where bacteria without ti-plasmid were applied, and where no calli were formed. after isolation of dna from hormone-independently growing cells furthe ...	1987	24276903
t-dna rearrangements due to tissue culture: somaclonal variation in crown gall tissues.	after three years of apparent stability in tissue culture, the single cell derived shooty crown gall line snt1.013 produced a revertant shoot which had switched from non-rooting (rod(+)) and octopine synthesizing (ocs(+)) to rod(-) ocs(-), indicating that in this revertant tl-dna genes 4 (causing the rod(+) trait) and gene 3 (causing the ocs(+) trait) had been inactivated. southern blots revealed that the inactivation of these t-dna genes was the result of a considerable rearrangement of dna seq ...	1987	24276797
β-1,3-glucanase class iii promotes spread of pvy(ntn) and improves in planta protein production.	glucanases are enzymes regulating the size exclusion limit and permeability of plasmodesmata and play a role in biotic stress. in plant genomes, they are encoded as relatively large gene families divided into four classes. most studies of plant virus interactions have focused on glucanases from classes i and ii. in our study, we have evaluated the role of the β-1,3-glucanase class iii (glu-iii) gene in the potato-potato virus y(ntn) (pvy(ntn)) interaction and implemented the findings to plant bi ...	2013	24273610
agrobacterium-mediated genetic transformation using cotyledons in japanese pear (pyrus pyrifolia).	genetic transformation was successfully established producing both transformed adventitious shoots and calli in japanese pear (pyrus pyrifolia nakai) by using cotyledons as explants. cotyledons of five cultivars were co-cultivated with agrobacterium tumefaciens strain lba4404 carrying the pbin19-sgfp, which contained a green fluorescent protein gene and the neomycin phosphotransferase gene. in order to increase transformation efficiency, sonication and ethylenedioxybis (ethylamine)-n,n,n',n'-tet ...	2013	24273422
transformation of homozygous diploid potato with an agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments.	transformed potato (solanum tuberosum) plants were obtained from homozygous diploid potato by using a transformation procedure in combination with an adventitious shoot regeneration method. leaf and stem explants were inoculated with an agrobacterium tumefaciens strain which contained a binary vector (pvu 1011) carrying the neomycin phosphotransferase gene. shoot regeneration most effectively on stem explants, occurred within six weeks directly from the explants without introducing a callus phas ...	1989	24272868
isolation and functional analysis of a set of auxin genes with low root-inducing activity from an agrobacterium tumefaciens biotype iii strain.	a new type of root-inducing iaa gene set was cloned from the ti plasmid of the biotype iii agrobacterium tumefaciens strain tm-4. these iaa genes are characterized by a very low dna homology with the well-characterized iaa gene set, iaam and iaah, of the "common dna" region of the biotype i strain ach5 and by a low root-inducing activity.the biological activities of both iaa gene sets were compared by transferring each into a disarmed ti vector and by testing the resulting strains on nicotiana r ...	1989	24272862
analysis of a chimeric class-i patatin-gus gene in transgenic potato plants: high-level expression in tubers and sucrose-inducible expression in cultured leaf and stem explants.	patatin is a family of lipid acyl hydrolases that accounts for 30 to 40% of the total soluble protein in potato (solanum tuberosum l.) tubers. to examine the regulation of the patatin genes, we constructed a chimeric gene containing 2.5 kb of 5' flanking sequence from the class i patatin genomic clone ps20 transcriptionally fused to β-glucuronidase (gus) and introduced it into potato plants using an agrobacterium tumefaciens tiplasmid vector. while the chimeric gene was expressed at high levels ...	1989	24272716
a stable and efficient agrobacterium tumefaciens-mediated genetic transformation of the medicinal plant digitalis purpurea l.	in this study, we developed a rapid and efficient method for in vitro propagation and agrobacterium tumefaciens-mediated transformation of digitalis purpurea l. (syn. foxglove), an important medicinal plant. mature leaf explants of d. purpurea were used for 100 % adventitious shoot regeneration on murashige and skoog (ms) medium supplemented with 1 mg l(-1) thidiazuron (tdz) (a cytokine) and 0.1 mg l(-1) 1-naphthaleneacetic acid (naa) (an auxin). transformation was achieved by inoculating leaf e ...	2014	24272685
cassava latent virus infections mediated by the ti plasmid of agrobacterium tumefaciens containing either monomeric or dimeric viral dna.	plant infections with cassava latent virus (clv) were mediated by the ti plasmid of agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. the clv dnas caused typical symptoms when they were inoculated in agrobacterium strains c58, lba4404 and a vire mutant a1026, but not other agrobacterium strains with mutations in other vir loci or an e. coli pola strain. virus-specific dna forms characteristic of normal clv infections were found after such infection. cha ...	1988	24272630
the agrobacterium tumefaciens t-dna gene 6(b) is an onc gene.	in this article it is shown that the t-dna of agrobacterium tumefaciens contains besides the well-known cyt and aux genes another gene with an oncogenic effect in plants. the gene in question is called 6(b) and causes the formation of small tumors in plant species such as nicotiana glauca and kalanchoe tubiflora.	1988	24272629
function of heterologous and pseudo border repeats in t region transfer via the octopine virulence system of agrobacterium tumefaciens.	the successful transfer of the ti plasmid t region to the plant cell is mediated by its 24 bp border repeats. processing of the t-region prior to transfer to the plant cell is started at the right border repeat and is stimulated by a transfer enhancer sequence called "overdrive". left and right border repeats differ somewhat in nucleotide sequence; moreover, the repeats of different ti and ri plasmids are slightly different. our data indicate that these differences do not have a significant infl ...	1988	24272627
common evolutionary origin of the central portions of the ri tl-dna of agrobacterium rhizogenes and the ti t-dnas of agrobacterium tumefaciens.	analysis of published sequences for ri tl-dna (root-inducing left-hand transferred dna) of agrobacterium rhizogenes revealed several unsuspected structural features. first, ri tl-dna genes are redundant. using redundancy as a criterion, three regions (left, middle and right) were discerned. the left one, orfs (open reading frames) 1-7, contains no detectable redundancy. in the middle region a highly diverged gene family was detected in orfs 8, 11, 12, 13 and 14. the right region contains an appa ...	1988	24272624
a modified storage protein is synthesized, processed, and degraded in the seeds of transgenic plants.	in vitro mutagenesis was used to supplement the sulfur amino acid codon content of a gene encoding β-phaseolin, a phaseolus vulgaris storage protein. the number of methionine codons in the phaseolin gene was increased from three to nine by insertion of a 45 base pair (bp) synthetic duplex. either modified or normal phaseolin genes were integrated into the genome of tobacco plants through agrobacterium tumefaciens-mediated transformation. although similar levels of phaseolin rna are detected in s ...	1988	24272623
influence of a crown-gall tumor initiation enhancer on bacterial attachment to the host plant cell wall.	the competitive activities of different plant cell walls upon agrobacterium tumefaciens attachment have been studied in vitro by means of two crown-gall tumor initiation assays. the low or high susceptibility of different plant species is independent of their capacity to cause bacterial cells to adhere to specific sites on the plant cell walls. however, the attachment properties of cell wall fragments derived from helianthus cotyledons seem to be age-dependent. it is found that a tumor initiatio ...	1982	24272582
evidence for the existence of a universal crown-gall tumor initiation enhancer.	tumor initiation of different dicotyledonous plant species inoculated with agrobacterium tumefaciens b6 has been studied in vivo and in vitro. the tumor formation in weakly susceptible plants can be strongly enhanced by exogenously applied active extract fractions derived from highly susceptible helianthus cotyledons. it is found that highly susceptible plants (kalanchoë, lycopersicon and pinto beans) contain an active tumor initiation enhancer which is clearly similar to the compound(s) found i ...	1982	24272581
the sequence of a pea vicilin gene and its expression in transgenic tobacco plants.	a 5.5 kb eco ri fragment containing a vicilin gene was selected from a pisum sativum genomic library, and the protein-coding region and adjacent 5' and 3' regions were sequenced. a dna construction comprising this 5.5 kb fragment together with a gene for neomycin phosphotransferase ii was stably introduced into tobacco using an agrobacterium tumefaciens binary vector, and the fidelity of expression of the pea vicilin gene in its new host was studied. the seeds of eight transgenic tobacco plants ...	1988	24272502
changes in the tissue-specific prevalence of translatable mrnas in transgenic tobacco shoots containing the t-dna cytokinin gene.	two-dimensional gel electrophoresis of in vitro translation products was used to examine differences between the steady state rna populations of an untransformed tobacco plant line and a non-rooting tobacco shoot line transformed with a t l -dna segment from agrobacterium tumefaciens carrying the cytokinin gene (t-cyt). the analysis comprised about 240 translation products representing the more abundant mrnas. approximately 8% of the translation products were found to have significantly differen ...	1988	24272496
regeneration of flax plants transformed by agrobacterium rhizogenes.	regeneration of flax (linum usitatissimum) following transformation by either agrobacterium tumefaciens carrying a disarmed ti-plasmid vector, or agrobacterium rhizogenes carrying an unmodified ri plasmid, was examined. hypocotyl and cotyledon explants inoculated with a. tumefaciens formed transformed callus, but did not regenerate transformed shoots either directly or via callus. however, cotyledon explants inoculated with a. rhizogenes formed transformed roots which did regenerate transformed ...	1988	24272489
right-border sequences enable the left border of an agrobacterium tumefaciens nopaline ti-plasmid to produce single-stranded dna.	the t-region of nopaline-type ti-plasmids (the portion of the plasmid that is transferred to plant cells) of agrobacterium tumefaciens is delimited by 23-25 bp direct repeats. they are nicked by the products of the vird locus and the presence of these nicked sites is correlated with the synthesis of single-stranded t-region copies. despite previous indications to the contrary, we show that the ptit37 t-region left border is capable of producing single-stranded dna with high efficiency and that i ...	1988	24272395
expression and inheritance of kanamycin resistance in a large number of transgenic petunias generated by agrobacterium-mediated transformation.	one hundred and four kanamycin-resistant petunia "mitchell" plants were regenerated from leaf discs cocultivated with agrobacterium tumefaciens strain lba4404 containing a binary vector pcgn200. selection for kanamycin resistance was applied during plant regeneration at the initiation of both shoots and roots. the regenerated plants were analysed for expression and inheritance of their kanamycin resistance phenotype. approximately half of the plants showed normal mendelian inheritance for one or ...	1988	24272348
molecular characterization of the virulence gene vira of the agrobacterium tumefaciens octopine ti plasmid.	the virulence loci play an essential role in tumor formation by agrobacterium tumefaciens. induction of vir gene expression by plant signal molecules is solely dependent on the virulence loci vira and virg. this study focused on the vira locus of the octopine type ti plasmid pti15955. the nucleic acid sequence of a 5.7-kilobase fragment encompassing vira was determined. genetic analysis of this region revealed that vira contains one open reading frame coding for a protein of 91 639 daltons. immu ...	1988	24272265
differential expression of conglycinin α' and β subunit genes in transgenic plants.	β-conglycinin or 7s protein, one of the two major storage proteins of soybean (glycine max), is comprised of three subunits, α', α and β. genomic clones encoding the α' and β subunit genes were ligated together in intermediate plasmids, either in direct orientation (← ←) or in divergent orientation (← →). in the latter instance the transcriptional promoters of the two genes were approximately 2.0 kb apart. the dnas were introduced into leaf disks of petunia hybrida on a disarmed ti plasmid in ag ...	1988	24272254
detection of a non-structural protein of m r 11 000 encoded by the virion dna of maize streak virus.	a polypeptide of approximately 11 000 daltons (11 kda protein) encoded by an open reading frame (10.9 orf) from the virion sense of maize streak virus (msv) dna has been detected among the products of in vitro translation reactions programmed with rna from infected maize plants and also in total protein extracts from infected leaves. the 11 kda protein has not been detected in virions and is therefore proposed to have a nonstructural role.viral dna with an additional in-frame translation stop co ...	1988	24272158
agroinfection and nucleotide sequence of cloned wheat dwarf virus dna.	cloned dna of the geminivirus wheat dwarf virus (wdv) was successfully used to infect seedling wheat plants. the clone was derived from circular double-stranded viral dna isolated from naturally infected tissue. the initiation of infection was mediated by agrobacterium tumefaciens using cloned dimeric wdv genomes in a binary agrobacterium vector. the wdv dna which comprised the infectious clone was sequenced and is compared with the published sequence of a swedish isolate of the same virus. the ...	1988	24272156
detection of genetically modified tomato using pcr coupled with muparaflo microfluidics microarrays.	genetically modified (gm) tomatoes have been approved for commercialization in many countries since the first gm tomato flavr savr was permitted for planting in 1994. to meet the requirement of the gm tomatoes labeling policy, in this study we firstly set up the conventional pcr and multiplex pcr detection system for screening the universal elements transformed into tomato, such as cauliflower mosaic virus 35s (camv 35s) promoter, nopaline synthase (nos) terminator of agrobacterium tumefaciens, ...	2013	24266223
from tumour to tuber; tumour cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (solanum tuberosum cv. 'maris bard').	agrobacterium tumefaciens strains, known to induce tobacco crown galls that spontaneously develop shoots, were used to induce galls on cultured shoots of a tetraploid potato cultivar (solanum tuberosum cv. 'maris bard'). shoots also appeared spontaneously from the induced potato galls, although only after 2-4 months. the shoots were excised and cultured separately. some of these frequently developed side-shoots from their axillary buds. they did not form roots and they produced opines, a strong ...	1983	24263772
generation of doubled haploid transgenic wheat lines by microspore transformation.	microspores can be induced to develop homozygous doubled haploid plants in a single generation. in the present experiments androgenic microspores of wheat have been genetically transformed and developed into mature homozygous transgenic plants. two different transformation techniques were investigated, one employing electroporation and the other co-cultivation with agrobacterium tumefaciens. different tissue culture and transfection conditions were tested on nine different wheat cultivars using ...	2013	24260351
overexpression of cytokinin dehydrogenase genes in barley (hordeum vulgare cv. golden promise) fundamentally affects morphology and fertility.	barley is one of the most important cereal crops grown worldwide. it has numerous applications, but its utility could potentially be extended by genetically manipulating its hormonal balances. to explore some of this potential we identified gene families of cytokinin dehydrogenases (ckx) and isopentenyl transferases, enzymes that respectively irreversibly degrade and synthesize cytokinin (ck) plant hormones, in the raw sequenced barley genome. we then examined their spatial and temporal expressi ...	2013	24260147
agartrap: a simplified agrobacterium-mediated transformation method for sporelings of the liverwort marchantia polymorpha l.	the liverwort marchantia polymorpha l. is being developed as an emerging model plant, and several transformation techniques were recently reported. examples are biolistic- and agrobacterium-mediated transformation methods. here, we report a simplified method for agrobacterium-mediated transformation of sporelings, and it is termed agar-utilized transformation with pouring solutions (agartrap). the procedure of the agartrap was carried out by simply exchanging appropriate solutions in a petri dis ...	2014	24259681
treatment of rhizobium radiobacter bacteremia in a critically ill trauma patient.	to report the first case of rhizobium radiobacter bacteremia in a critically ill trauma patient.	2013	24259594
induction and in vitro culture of soybean crown gall tumors.	induction of crown galls on 4-6 week old soybean (glycine max (l.) merr.) plants cultured in growth chambers was obtained with agrobacterium tumefaciens strains c58, t37 and ach5. the crown galls were isolated and cultured in vitro as sterile callus and liquid suspension cultures. transformation was tested by opine tests and by molecular hybridization of restricted cell dna with t-dna fragments. protoplasts were isolated from suspension cultures. transformed protoplasts regenerate cell walls, di ...	1983	24258052
cytokinins secreted by agrobacterium promote transformation by repressing a plant myb transcription factor.	agrobacterium-mediated transformation is the most widely used technique for generating transgenic plants. however, many crops remain recalcitrant. we found that an arabidopsis myb family transcription factor (mtf1) inhibited plant transformation susceptibility. mutating mtf1 increased attachment of several agrobacterium strains to roots and increased both stable and transient transformation in both susceptible and transformation-resistant arabidopsis ecotypes. cytokinins from agrobacterium tumef ...	2013	24255177
electric field mediated stable transformation of carrot protoplasts with naked dna.	we have developed an electroporation procedure for the transformation of carrot protoplasts with ti-plasmid dna from agrobacterium tumefaciens. the uptake of ptic58 into carrot protoplasts was mediated by high voltage electrical pulses at field strengths from 0.5 to 3.8 kv/cm. protoplast regeneration, somatic embryogenesis and plantlet regeneration were unaffected by the electroporation conditions selected for dna uptake. uptake of plasmid ptic58 resulted in hormone independent regeneration of c ...	1985	24254082
brassica grown gall tumourigenesis and in vitro of transformed tissue.	a number of brassica species and cultivars were tested and found to be highly susceptible to crown gall induction by both nopaline and octopine strains of agrobacterium tumefaciens. only b. napus did not form tumours when inoculated with octopine strains. seedlings of very young plants were poor hosts but efficient infection occurred after 8-10 weeks of growth. teratomas arising on tumours in planta were relatively frequent on induction with nopaline strains. axenically cultured tumour calli of ...	1985	24254075
transformation of protoplast-derived cell colonies and suspension cultures by agrobacterium tumefaciens.	in this paper we describe procedures for transforming micro colonies derived from mesophyll protoplasts of petunia hybrida with agrobacterium tumefaciens. the method is efficient, up to 70% of the colonies were transformed, and we used a similar method to transform cells from a suspension culture of haploid nicotiana plumbaginifolia.	1985	24253882
ethylene production by shoot-forming and unorganized crown-gall tumor tissues of nicotiana and lycopersicon cultured in vitro.	we have studied ethylene biosynthesis in cloned crown-gall cell lines of nicotiana tabacum l., n. glutinosa l., and lycopersicon esculentum (l.) mill. transformed by the a6 strain of agrobacterium tumefaciens (smith and townsend) conn. or a tms (shooty) mutant strain, a66. both the synthesis of the ethylene precursor 1-aminocyclo-propane-1-carboxylic acid (acc) and the conversion of acc to ethylene were affected by crown-gall transformation. all a6-transformed cell lines contained about 50 times ...	1984	24253841
fusion of agrobacterium and e. coli spheroplasts with nicotiana tabacum protoplasts - direct gene transfer from microorganism to higher plant.	spheroplasts of agrobacterium tumefaciens strains and e. coli were fused with protoplasts of nicotiana tabacum. fusion products were cultured in the presence of antibiotics to eliminate remaining bacterial spheroplasts. on hormone free medium, tobacco protoplasts treated with wild type agrobacterium-strains formed colonies with an average frequency of 10(-4). opine synthesis was detected in the tissues. some calli derived from protoplasts treated with a. tumefaciens c58c1pri15834 formed typical ...	1984	24253394
transformation of lettuce (lactuca sativa) mediated by agrobacterium tumefaciens.	lactuca sativa can be routinely transformed using ti plasmids of agrobacterium tumefaciens containing a chimeric kanamycin resistance gene (nos.nptii.nos). critical experimental variables were plant genotype, bacterial concentration, presence of a nurse culture and timing of transfers between tissue culture media. transformation was confirmed by the ability to callus and root in the presence of kanamycin, nopaline production, and by hybridization in southern blots. transformation has been achiev ...	1987	24248927
genetic transformation of flax (linum usitatissimum) by agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase.	genetic transformation of flax (linum usitatissimum) has been achieved using an a. tumefaciens strain carrying a non-oncogenic ti plasmid-derived vector containing a chimaeric npt-ii gene and a wild type nopaline synthase gene. fertile, transformed shoots were most easily obtained from km(r) callus developing on hypocotyl sections. the totipotency of the km(r) callus was dependent upon its origin. t-dna was visualised by southern blotting in all km(r) tissues. efficient expression of nopaline sy ...	1987	24248856
transformation of brassica napus with agrobacterium tumefaciens based vectors.	a reproducible system to produce transgenic brassica napus plants has been developed using stem segments. stem segments from 6-7 week old plants were inoculated with an agrobacterium tumefaciens strain containing a disarmed tumor-inducing plasmid ptit37-se carrying a chimeric bacterial gene encoding kanamycin resistance (pmon200). stem explants were cocultured for 2 days before transfer to kanamycin selection medium. shoots regenerated directly from the explant in 3-6 weeks and were excised, dip ...	1987	24248835
correlation between binding of agrobacterium tumefaciens by root cap cells and susceptibility of plants to crown gall.	we compared the binding of agrobacterium tumefaciens by freshly isolated root cap cells with susceptibility of plants to crown gall tumorigenesis. a high binding reaction was strongly correlated with susceptibility to tumorigenesis in a survey of the binding of strain b6 to cells from 48 species in 17 families. in reciprocal experiments with nine virulent a. tumefaciens strains, tumors developed in plant-bacteria combinations that gave a high binding response in the root cap cell assay. binding ...	1987	24248761
transformation of stylosanthes spp. using agrobacterium tumefaciens.	tumours were incited on leaf sections of stylosanthes humilis, s. hamata, s. guianensis and s. scalra following infection by agrobacterium tumefaciens. the suitability of 2 binary vectors (pga472, bin6) for gene transfer in s. humilis was tested and kanamycin-resistant tumour tissue was obtained from infected leaf pieces. the presence and expression of the neomycin phosphotransferase (npt ii) gene in the plant cells was demonstrated by hybridization of the coding region of the npt ii gene of the ...	1987	24248653
transformation of cultivated tomato by a binary vector in agrobacterium rhizogenes: transgenic plants with normal phenotypes harbor binary vector t-dna, but no ri-plasmid t-dna.	cultivated tomato was genetically transformed using two procedures. in the first procedure, punctured cotyledons were infected with "disarmed" agrobacterium tumefaciens strain lba4404 or with a. rhizogenes strain a4, each containing the binary vector parc8. the chimeric neomycin phosphotransferase (npt ii) gene on parc8 conferred on transformed plant cells the ability to grow on medium containing kanamycin. transformation reproducible yielded kanamycin-resistant transformants in different tomato ...	1986	24248198
transformation of medicago by agrobacterium mediated gene transfer.	shoot segments of medicago varia genotype a2 were co-cultivated with agrobacterium tumefaciens strain bo42 carrying pga471, a plasmid coding for the kanamycin resistant determinant as transferable positive selection marker in plant cells (an et al., 1985). resistant plants were regenerated at high frequency from green calli developed on inoculated stem cuttings under kanamycin selection. dna-dna hybridization analysis showed the presence of the structural gene of the kanamycin resistant determin ...	1986	24248043
leaf disc transformation of cultivated tomato (l. esculentum) using agrobacterium tumefaciens.	the leaf disc transformation/regeneration system was modified for tomato (l. esculentum). both leaf explants and cotyledon/hypocotyl sections can be used to regenerate transformed plants. we have obtained over 300 transgenic plants from eight tomato cultivars. we have evidence for both single and multi-copy insertions of the t-dna, and have demonstrated inheritance of the t-dna insert in the expected mendelian ratios. several heterologous promoters function in tomato. a reduced efficiency of tra ...	1986	24248039
isozyme gene expression in potato tumors incited by agrobacterium.	two plant tumors (crown galls and hairy roots) were experimentally provoked on potato cv. 'désirée' by oncogenic strains of agrobacterium tumefaciens and a. rhizogenes. a marked shift in the expression of some organ-specific genes occurred in crown galls derived from the central zone of tubers: two novel isozyme genes (est-b and pox-e) were expressed, two others (est-c and pox-f) were suppressed and the remaining ones were maintained in the original state. when the starting tissue was the stem s ...	1986	24247945
shoot regeneration of mesophyll protoplasts transformed by agrobacterium tumefaciens, not achievable with untransformed protoplasts.	alternative methods for shoot regeneration in protoplast derived cultures were developed in nicotiana paniculata and physalis minima. in both species protoplast derived callus is not regeneratable to shoots by conventional methods, e.g. hormone treatment. leaf discs and stem segments of n. paniculata and p. minima were incubated with agrobacterium tumefaciens "shooter" strains harbouring pgv 2215 or pgv 2298 or wildtype strain b6s3. after 36 h of co-incubation protoplasts were prepared. (leaf di ...	1986	24247786
transformation of forage legumes using agrobacterium tumefaciens.	galls were induced in six species of forage legumes following inoculation with wild-type strains of a. tumefaciens. the plant species was more influential than the bacterial strain in determining the type of tumour produced. inoculation of medicago sativa resulted in small, disorganised tumours. the three trifolium species, t. repens, t. hybridum and t. pratense, formed galls which tended to produce roots and both onobrychis viciifolia and lotus corniculatus produced teratomatous galls. the shoo ...	1986	24247771
genetic manipulation in cultivars of oilseed rape (brassica napus) using agrobacterium.	the response of oilseed rape cultivars to infection with agrobacterium tumefaciens and a. rhizogenes and the possibility of regenerating genetically transformed oilseed rape plants were examined. the frequency at which agrobacterium induced galls or hairy-roots on in vitro cultured plants ranged from 10% to 70%, depending on the cultivar. from galls induced by the tumorigenic strain t37, known to be strongly shoot inducing on tobacco, roots developed frequently. occasionally, shoots formed and s ...	1985	24247402
agrobacterium-mediated transformation of maize (zea mays) immature embryos.	agrobacterium tumefaciens-mediated transformation is one of the most efficient and simple gene delivery systems for genetic improvement and biology studies in maize. this system has become more widely used by both public and private laboratories. however, transformation efficiencies vary greatly from laboratory to laboratory for the same genotype. here, we illustrate our advanced agrobacterium-mediated transformation method in hi-ii maize using simple binary vectors. the protocol utilizes immatu ...	2014	24243211
agrobacterium-mediated transformation: rice transformation.	agrobacterium is a common soil bacterium with natural capacity for trans-kingdom transfer of genetic information by transferring its t-dna into the eukaryotic genome. in agricultural plant biotechnology, combination of non-phytopathogenic strain of agrobacterium tumefaciens with modified t-dna and vir-genes in a binary vector system is the most widely utilized system for genetic improvement in diverse plant species and for gene function validation. here we have described a highly efficient a. tu ...	2014	24243210
genetic transformation of wheat via agrobacterium-mediated dna delivery.	the method described involves an initial incubation of wheat immature embryos in a liquid culture of agrobacterium tumefaciens. the agrobacterium strain is engineered to contain a binary vector with a gene of interest and a selectable marker gene placed between the t-dna borders; the t-dna is the region transferred to the plant cells, thus harnessing the bacterium's natural ability to deliver specific dna into host cells. following the initial inoculation with the agrobacterium, the embryos are ...	2014	24243208
transformed callus does not necessarily regenerate transformed shoots.	agrobacterium tumefaciens carrying a disarmed ti plasmid vector was used for the transformation of flax tissue. transformed callus was obtained from inoculated hypocotyl segments and healthy green shoots were regenerated from this callus. nopaline assays on shoot tissue were positive for nopaline content if carried out soon after removal of the shoot from the callus but negative if carried out 2-3 weeks after removal. all of these shoots gave rise to kanaymcin sensitive progeny and were most lik ...	1988	24241768
glyphosate tolerant flax plants from agrobacterium mediated gene transfer.	agrobacterium tumefaciens carrying a disarmed ti-plasmid vector containing a chimeric npt-ii gene and a glyphosate resistance plant-derived 5-enolpyruvylshikimate-3-phosphate synthase gene was used to transform flax hypocotyl tissues. transformed shoots could be regenerated from the inoculated tissue and were proven to be transgenic by the combination of leaf callus assays, nopaline assays and progeny tests. co-segregation was observed in the progeny for kanamycin and glyphosate resistance.	1988	24241767
genetic transformation of 9 in vitro clones of alnus and betula by agrobacterium tumefaciens.	crown gall tumorigenesis, integration and expression of t-dna encoded genes from agrobacterium tumefaciens were investigated in 9 clones of alnus glutinosa, a. incana and betula papyrifera. tumor formation on in vitro shoots was frequent in all clones with strain ach5 and present in 8 clones with strain c58. tumors excised from shoots were selected for autotrophic growth in vitro and axenic cultures were established. octopine or nopaline, respective of the strain type used for inoculation, was d ...	1988	24241754
celery transformation by agrobacterium tumefaciens: cytological and genetic analysis of transgenic plants.	transgenic celery plants were obtained following co-cultivation of petiole explants with agrobacterlum tumefaciens containing pmon200, a cointegrate vector carrying genes for kanamycin resistance and nopaline synthase. transformants were selected by ability of callus to grow in the presence of 50mg/l kanamycin. transformation was confirmed either by the presence of nopaline or by southern blots. cytological analysis of 14 transformed plants revealed chromosomal aberrations, both in structure and ...	1988	24241542
the role of bacterial attachment in the transformation of cell-wall-regenerating tobacco protoplasts by agrobacterium tumefaciens.	the presence of a newly formed primary cell wall was shown to be required for attachment and subsequent transformation of tobacco leaf protoplasts by agrobacterium tumefaciens in cocultivation experiments. in these experiments both protoplasts at different stages after their isolation and cell-wall inhibitors were used. the specificity of agrobacterium attachment was shown by using other kinds of bacteria that did not attach. by diminishing the concentration of divalent cations using ethylenedia ...	1985	24241510
callus induction from protoplasts of v. unguiculata, v. sublobata and v. mungo.	protoplasts were isolated from hypocotyl of v. mungo (l.) hepper or hypocotyl-derived callus of v. sublobata (phaseolus sublobata roxb.) and v. unguiculata (l.) walp (syn. v. sinensis (l.) saviex hassk) using an enzyme solution comprising cellulase 2.5%, macerozyme, hemicellulase and driselase each at a 0.5% level in 0.5 m sorbitol. isolated protoplasts were cultured in murashige and skoog's (1962) basal liquid medium supplemented with ba, naa, 2,4-d (1 mg/l each) and sucrose (14%). after four w ...	1987	24241464
introduction of foreign genes into potato cultivars bintje and désirée using an agrobacterium tumefaciens binary vector.	tuber discs of solanum tuberosum cv bintje and désirée were cocultivated with an agrobacterium tumefaciens binary vector, carrying both the neomycine phosphotransferase and the e. coli β-glucuronidase gene fused to resp. the nopaline synthase and cauliflower mosaic virus 35s promotor.inoculated tuber discs produce transgenic shoots in selective media containing kanamycin. the transgenic plants are phenotypically normal and contain the euploid number of chromosomes. both the neomycin phosphotrans ...	1988	24241414
a rapid transformation method for solanum tuberosum using binary agrobacterium tumefaciens vectors.	a tuber disc transformation and regeneration system was devised for potato (solanum tuberosum). tuber discs were found to be the most morphogenetic organ on a medium previously optimised for tomato regeneration. shoot regeneration from tuber discs was rapid and transformed as shown by nopaline assays and southern blot analysis. the ease and speed of the tuber disc method will allow for the increased use of this commercially important plant in transformation studies.	1988	24241405
genetic transformation in two potato cultivars with t-dna from disarmed agrobacterium.	derivatives of potato (solanum tuberosum cv.'s 'maris bard' and 'desiree') transformed with disarmed t-dna from genetically engineered agrobacterium tumefaciens strains were isolated. the transformed plants were recovered from shoot-forming tumours induced by infection of wounds with mixedcultures of shoot-inducing a. tumefaciens strains t37 and either agrobacterium strain lba1834(pral1834), (hille et al. 1983) or lba4404(pbin6; pral4404), (bevan 1984). two small-scale feasibility experiments ga ...	1987	24241200
genetic transformation of apple (malus pumila mill.) using a disarmed ti-binary vector.	the disamed ti-binary vector pbin 6 in agrobacterium tumefaciens has been used in leaf disc transfomations to produce transgenic apple (malus pumila mill.) plants with a nomal phenotype except for a somewhat reduced capacity to root. the presence of the genes for nopaline synthase and neomycin phosphotrans ferase (conferring kanamycin resistance), inserted into the host genome by the vector, was confirmed by southern blot analysis, the detection of nopaline synthase activity and rooting in the p ...	1989	24240455
biotransformation of nicotine alkaloids by tobacco shooty teratomas induced by a ti plasmid mutant.	tobacco shooty or rooty teratomas and hairy roots were induced by agrobacterium tumefaciens (pgv 3845), a. tumefaciens (pgv 3304) and a. rhizogenes (pri 8196), respectively. the tobacco alkaloids, nicotine, nornicotine and anatabine, were produced in hairy roots and in rooty teratomas but not in shooty teratomas. however, the shooty teratomas have the ability to accumulate the alkaloids and to biotransform nicotine to nornicotine. these were established by co-culture experiments incubating hairy ...	1989	24240441
high efficiency agrobacterium tumefaciens-mediated transformation of arabidopsis thaliana leaf and cotyledon explants.	a highly efficient and fast agrobacterium-mediated leaf disc transformation system for the arabidopsis thaliana l. genotype c24 was developed. this protocol is also amenable to other ecotypes - as could be shown for landsberg erecta and wassllewskija. besides the hygromycin selection also the g418 and kanamycin selection were established. furthermore the described procedure is appliable not only to leaf explants but also to expanded cotyledons which proved to be an excellent alternative as expla ...	1988	24240423
parameters affecting the frequency of kanamycin resistant alfalfa obtained by agrobacterium tumefaciens mediated transformation.	kanamycin resistant plants of medicago varia a2 were obtained by an optimized procedure for high frequency transformation using agrobacterium tumefaciens infection of leaf and petiole tissue. parameters which affected the frequency were explant type, the agrobacterium strain used and the time allowed for cocultivation. under optimum conditions, i.e., using the agrobacterium strain a281 and a 4 day cocultivation period, the frequency of transformed leaflets obtained was greater than 70%.	1988	24240404
transformation and regeneration of brassica oleracea mediated by an oncogenic agrobacterium tumefaciens.	a chimaeric neomycin phosphotransferase ii (npt ii) gene was introduced in brassica oleracea using an oncogenic strain of agrobacterium tumefaciens harbouring ti plasmid which contains nos/nptii in its t-dna. the transformation of b. oleracea with the oncogenic ti plasmid, resulted in regeneration of shoots and roots without any exogenous requirement of phytohormones. the presence of npt ii gene was determined by hybridization of tn5 encoded npt ii gene with dna of kanamycin resistant regenerate ...	1988	24240402
variability in tissue cultures of choisya ternata. iii comparing alkaloid production in cell lines obtained by various strategies.	callus cultures of choisya ternata have been prepared by different strategies: aggregate clones, subclones and protoclones obtained from well-established strains; protoclones obtained from mesophyll tissue; cultures transformed by agrobacterium tumefaciens. all of them show high variability in their dihydrofuroquinoline alkaloid production. as compared to the alkaloid content of the whole plant, one alkaloid (platydesminium) could be obtained in higher amounts in some lines, but it was impossibl ...	1988	24240269
agrobacterium mediated transfer of chlorsulfuron resistance to commercial flax cultivars.	agrobacterium tumefaciens carrying a disarmed ti-plasmid vector containing a chimeric npt-ll gene and a mutant acetolactate synthase gene (conferring resistance to the herbicide chlorsulfuron) from arabidopsis was used to transform flax (linum usitatissimum) hypocotyl tissue. transgenic regenerants were recovered from the inoculated tissue and were tested for expression of the foreign genes by leaf callus assays on kanamycin and on chlorsulfuron. transgenic plants were grown to maturity; selfed ...	1989	24233525
successful cocultivation of brassica napus microspores and proembryos with agrobacterium.	brassica napus microspores and microspore-derived proembryos were cocultivated with agrobacterium tumefaciens harbouring a binary vector. the vector contained selectable genes for kanamycin and hygromycin antibiotic resistance. microspores and proembryos survived the cocultivation procedure and subsequent antibiotic selection. thousands of plantlets can be regenerated from a single experiment. biochemical analysis indicated up to 7.3% of plants exhibited neomycin phosphotransferase ii enzyme act ...	1989	24233359
virulence of agrobacterium tumefaciens strains with brassica napus and brassica juncea.	brassica napus and brassica juncea were infected with a number of agrobacterium tumefaciens strains. tumourigenesis was very rapid and extremely efficient on b. juncea with all but one of the strains. tumourigenesis on b. napus varied widely. it was very efficient with the nopaline strains, was reduced with the succinamopine strain a281 and was very weak with the octopine strains. the latter observation was confirmed with six different b. napus rapeseed cultivars. the selectivity was due to diff ...	1989	24233232
specificity of strain and genotype in the susceptibility of pea to agrobacterium tumefaciens.	to determine the best combination for potential use in transformation of pisum sativum l., 13 genotypes were inoculated with wild-type agrobacterium tumefaciens strains a281, c58 and ach5. a281 appeared to be the most virulent strain, as determined by size and number of tumours, followed by c58 and ach5. genotypes differed considerably in their response to inoculation and genotype x strain interaction was evident. genotypes also responded differently to in vivo or in vitro inoculation. axenic ca ...	1989	24233224
detection of bacterial chitinase activity in transformed plant tumour cells using a specific exochitinase substrate.	methods for the detection of bacterial chitinase activity were compared. the soluble substrate p-nitrophenyl-ß-d-n,n diacetyl chitobiose (ndc) was more sensitive in detecting purified chitinase of serratia marcescens than assays measuring degradation of a solid chitin substrate by either radiochemical or colorimetric means. a chimaeric gene containing a s. marcescens chitinase gene under control of a cauliflower mosaic virus 35s promoter and nopaline synthase terminator sequences was constructed ...	1990	24232928
genetic transformation of medicago species by agrobacterium tumefaciens and electroporation of protoplasts.	shoot and leaf segments of a non-regenerable medicago sativa l. genotype were cocultivated with the "shooty" mutant of agrobacterium tumefaciens carrying the pgv 2206 plasmid. transformed callus lines were selected and regenerated on the hormone free b5 medium. southern blot analysis demonstrated integration of t-dna in to the genome of the regenerated plants.transgenic plants resistant to kanamycin were obtained by electroporation of medicago borealis protoplasts with the pga 472 plasmid dna.	1990	24232780
production of terpenes by differentiated shoot cultures of mentha citrata transformed with agrobacterium tumefaciens t37.	crown gall initiation on mentha × piperita var. citrata (ehrh.) briq. (mint) was investigated using a range of wild type and mutant strains of agrobacterium tumefaciens. axenic transformed shoot cultures of mentha 'citrata' were established on plant stems inoculated with the nopaline strain t37 of agrobacterium tumefaciens. the presence of t-dna in the transformed tissues and the absence of bacterial contamination was established by southern blot hybridisation, using (32)p labelled fragments of ...	1990	24232681
neoplastic progression in crown gall in tobacco without elevated auxin levels.	we have isolated two stable variants from a crown-gall teratoma tissue of tobacco (nicotiana tabacum l.) transformed by agrobacterium tumefaciens strain a66, a mutant of the virulent a6 strain containing an insertion sequence in the tumor-inducing (ti) plasmid at the locus coding for auxin biosynthesis. normally tobacco cells transformed by strain a66 spontaneously form shoots in culture and will not grow on hormone-free medium unless shoots develop. the variant tissue lines, isolated from the t ...	1986	24232660
genotype-independent leaf disc transformation of potato (solanum tuberosum) using agrobacterium tumefaciens.	leaves of the in vitro grown potato cultivars 'bintje', 'berolina', 'desiree', and 'russet burbank' were wounded and co-cultivated with agrobacterium strains having chimeric bar and nptii genes on a disarmed t-dna. each leaf from these cultivars formed numerous calli on kanamycin-containing medium, and almost all calli regenerated shoots. for 'russet burbank', it was necessary to include agno3 in the medium to obtain efficient shoot regeneration. the transformed plants have one to a few copies o ...	1988	24232356
endogenous indoles and the biosynthesis and metabolism of indole-3-acetic acid in cultures of rhizobium phaseoli.	gas chromatography-mass spectrometric analyses of purified extracts from cultures of rhizobium phaseoli wild-type strain 8002, grown in a non-tryptophan-supplemented liquid medium, demonstrated the presence of indole-3-acetic acid (iaa), indole-3-ethanol (iet), indole-3-aldehyde and indole-3-methanol (im). in metabolism studies with (3)h-, (14)c- and (2)h-labelled substrates the bacterium was shown to convert tryptophan to iet, iaa and im; iet to iaa and im; and iaa to im. indole-3-acetamide (ia ...	1987	24227443
structure, organization and expression of transferred dna in nicotiana plumbaginifolia crown-gall tissues.	data are provided which show that transferred dna (t-dna) present in nicotiana plumbaginifolia crown-gall lines in most cases was scrambled and not intact. both wild-type, and 'rooter'- and 'shooter'-type mutants of octopine-type agrobacterium tumefaciens were used to infect n. plumbaginifolia plantlets, cultured in vitro. resulting tumors were excised from the plantlets and cultured for more than three years. during subculturing the tumor lines were scored for the following phenotypic traits: p ...	1987	24227439
incorporation of hygromycin resistance in brassica nigra and its transfer to b. napus through asymmetric protoplast fusion.	with the idea to develop a selection system for asymmetric somatic hybrids between oilseed rape (brassica napus) and black mustard (b. nigra), the marker gene hygromycin resistance was introduced in this last species by protoplast transformation with the disarmed agrobacterium tumefaciens strain c58 pgv 3850 hpt. the b. nigra lines used for transformation had been previously selected for resistance to two important rape pathogens (phoma lingam, plasmodiophora brassicae). asymmetric somatic hybri ...	1989	24227144
heavy metal tolerant transgenic brassica napus l. and nicotiana tabacum l. plants.	a chimeric gene containing a cloned human metallothionein-ii (mt-ii) processed gene was introduced into brassica napus and nicotiana tabacum cells on a disarmed ti-plasmid of agrobacterium tumefaciens. transformants expressed mt protein as a mendelian trait and in a constitutive manner. seeds from self-fertilized transgenic plants were germinated on media containing toxic levels of cadmium and scored for tolerance/ susceptibility to this heavy metal. the growth of root and shoot of transformed s ...	1989	24227139
transformation of cultured cells of chenopodium quinoa by binary vectors that carry a fragment of dna from the virulence region of ptibo542.	a 15.2-kb kpni fragment from the virulence region of ptibo542, the ti plasmid harbored by agrobacterium tumefaciens strain a281, was introduced into binary vectors. the fragment contained the virb, virc and virg genes, and it is known to have the ability to increase the virulence of strains of a. tumefaciens. the strains of a. tumefaciens that carried the resulting plasmids were able to transform cells in a suspension culture of chenopodium quinoa willd cells which were not transformable by comm ...	1990	24226938