Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| an examination of co-infection in acute gastroenteritis and histo-blood group antigens leading to viral infection susceptibility. | the aim of the present study was to evaluate co-infection in the gastrointestinal tract in terms of viruses, bacteria and the abo blood group. we hypothesized that a combination of norovirus (nv) and bacteria in the gastrointestinal tract could affect the likelihood of an individual to contracting nv. histo-blood group antigens (hbgas) are considered to act as receptors that can lead to nv susceptibility. in addition to genetics, co-infection in the gastrointestinal tract may be associated with ... | 2016 | 26998270 |
| discriminative power of campylobacter phenotypic and genotypic typing methods. | the aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of campylobacter in food. campylobacter jejuni (n=94) and campylobacter coli (n=52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (mlst), flagellin gene a restriction fragment length polymorphism typing (flaa-rflp), antimicrobial resistance profiling (amrp), the presence/absence of 5 putative virulence genes; and, exclusively for ... | 2016 | 26996762 |
| detection of campylobacter in human and animal field samples in cambodia. | campylobacter are zoonotic bacteria and a leading cause of human gastroenteritis worldwide with campylobacter jejuni and c. coli being the most commonly detected species. the aim of this study was to detect campylobacter in humans and livestock (chickens, ducks, pigs, cattle, water buffalo, quail, pigeons and geese) in rural households by routine culturing and multiplex pcr in faecal samples frozen before analysis. of 681 human samples, 82 (12%) tested positive by pcr (c. jejuni in 66 samples an ... | 2016 | 26991032 |
| whole-genome sequences of two campylobacter coli isolates from the antimicrobial resistance monitoring program in colombia. | campylobacter coli, along with campylobacter jejuni, is a major agent of gastroenteritis and acute enterocolitis in humans. we report the whole-genome sequences of two multidrug-resistance c. coli strains, isolated from the colombian poultry chain. the isolates contain a variety of antimicrobial resistance genes for aminoglycosides, lincosamides, fluoroquinolones, and tetracycline. | 2016 | 26988048 |
| a bacterial glycoengineered antigen for improved serodiagnosis of porcine brucellosis. | brucellosis is a highly zoonotic disease that affects animals and human beings. brucella suis is the etiological agent of porcine brucellosis and one of the major human brucellosis pathogens. laboratory diagnosis of porcine brucellosis mainly relies on serological tests, and it has been widely demonstrated that serological assays based on the detection of anti o-polysaccharide antibodies are the most sensitive tests. here, we validate a recombinant glycoprotein antigen, an n-formylperosamine o-p ... | 2016 | 26984975 |
| identification of e-cadherin signature motifs functioning as cleavage sites for helicobacter pylori htra. | the cell adhesion protein and tumour suppressor e-cadherin exhibits important functions in the prevention of gastric cancer. as a class-i carcinogen, helicobacter pylori (h. pylori) has developed a unique strategy to interfere with e-cadherin functions. in previous studies, we have demonstrated that h. pylori secretes the protease high temperature requirement a (htra) which cleaves off the e-cadherin ectodomain (ntf) on epithelial cells. this opens cell-to-cell junctions, allowing bacterial tran ... | 2016 | 26983597 |
| de novo asymmetric synthesis of a 6-o-methyl-d-glycero-l-gluco-heptopyranose-derived thioglycoside for the preparation of campylobacter jejuni nctc11168 capsular polysaccharide fragments. | an enantioselective de novo synthesis of a thioglycoside derivative of the 6-o-methyl-d-glycero-l-gluco-heptopyranose residue found in the campylobacter jejuni nctc11168 (hs:2) capsular polysaccharide is reported. the compound is obtained from a furfural-derived chiral diol in 11 steps. notably, compared to the only previous synthesis of this molecule, this approach significantly reduces the number of purification steps required to obtain the target. | 2016 | 26982173 |
| oral phage therapy of acute bacterial diarrhea with two coliphage preparations: a randomized trial in children from bangladesh. | antibiotic resistance is rising in important bacterial pathogens. phage therapy (pt), the use of bacterial viruses infecting the pathogen in a species-specific way, is a potential alternative. | 2016 | 26981577 |
| biofilm morphotypes and population structure among staphylococcus epidermidis from commensal and clinical samples. | bacterial species comprise related genotypes that can display divergent phenotypes with important clinical implications. staphylococcus epidermidis is a common cause of nosocomial infections and, critical to its pathogenesis, is its ability to adhere and form biofilms on surfaces, thereby moderating the effect of the host's immune response and antibiotics. commensal s. epidermidis populations are thought to differ from those associated with disease in factors involved in adhesion and biofilm acc ... | 2016 | 26978068 |
| influence of commercial laying hen housing systems on the incidence and identification of salmonella and campylobacter. | the housing of laying hens is important for social, industrial, and regulatory aspects. many studies have compared hen housing systems on the research farm, but few have fully examined commercial housing systems and management strategies. the current study compared hens housed in commercial cage-free aviary, conventional cage, and enriched colony cage systems. environmental and eggshell pool samples were collected from selected cages/segments of the housing systems throughout the production cycl ... | 2016 | 26976901 |
| diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold. | although it is known that diverse bacterial flagellar motors produce different torques, the mechanism underlying torque variation is unknown. to understand this difference better, we combined genetic analyses with electron cryo-tomography subtomogram averaging to determine in situ structures of flagellar motors that produce different torques, from campylobacter and vibrio species. for the first time, to our knowledge, our results unambiguously locate the torque-generating stator complexes and sh ... | 2016 | 26976588 |
| bacterial antisense rnas are mainly the product of transcriptional noise. | cis-encoded antisense rnas (asrnas) are widespread along bacterial transcriptomes. however, the role of most of these rnas remains unknown, and there is an ongoing discussion as to what extent these transcripts are the result of transcriptional noise. we show, by comparative transcriptomics of 20 bacterial species and one chloroplast, that the number of asrnas is exponentially dependent on the genomic at content and that expression of asrna at low levels exerts little impact in terms of energy c ... | 2016 | 26973873 |
| polynucleotide phosphorylase regulates multiple virulence factors and the stabilities of small rnas rsmy/z in pseudomonas aeruginosa. | post-transcriptional regulation enables bacteria to quickly response to environmental stresses. polynucleotide phosphorylase (pnpase), which contains an n-terminal catalytic core and c-terminal rna binding kh-s1 domains, is involved in rna processing. here we demonstrate that in pseudomonas aeruginosa the kh-s1 domains of pnpase are required for the type iii secretion system (t3ss) and bacterial virulence. transcriptome analysis revealed a pleiotropic role of pnpase in gene regulation. particula ... | 2016 | 26973625 |
| status of vaccine research and development for campylobacter jejuni. | campylobacter jejuni is one of the leading causes of bacterial diarrhea worldwide and is associated with a number of sequelae, including guillain-barre syndrome, reactive arthritis, irritable bowel syndrome and growth stunting/malnutrition. vaccine development against c. jejuni is complicated by its antigenic diversity, a lack of small animal models, and a poor understanding of the bacterium's pathogenesis. vaccine approaches have been limited to recombinant proteins, none of which have advanced ... | 2016 | 26973064 |
| expanding the role of fura as essential global regulator in cyanobacteria. | in the nitrogen-fixing heterocyst-forming cyanobacterium anabaena sp. pcc 7120, the ferric uptake regulator fura plays a global regulatory role. failures to eliminate wild-type copies of fura gene from the polyploid genome suggest essential functions. in the present study, we developed a selectively regulated fura expression system by the replacement of fura promoter in the anabaena sp. chromosomes with the co2+/zn2+ inducible coat promoter from synechocystis sp. pcc 6803. by removing co2+ and z ... | 2016 | 26967347 |
| interactions between intestinal pathogens, enteropathy and malnutrition in developing countries. | this review focuses on recent data highlighting the interactions between intestinal pathogens, enteropathy and malnutrition in developing countries, which drive morbidity and mortality and hinder the long-term developmental potential of children. | 2016 | 26967147 |
| multicenter evaluation of clinical diagnostic methods for detection and isolation of campylobacter spp. from stool. | the use of culture-independent diagnostic tests (cidts), such as stool antigen tests, as standalone tests for the detection of campylobacter in stool is increasing. we conducted a prospective, multicenter study to evaluate the performance of stool antigen cidts compared to culture and pcr for campylobacter detection. between july and october 2010, we tested 2,767 stool specimens from patients with gastrointestinal illness with the following methods: four types of campylobacter selective media, f ... | 2016 | 26962088 |
| atypical sensors for direct and rapid neuronal detection of bacterial pathogens. | bacterial infection can threaten the normal biological functions of a host, often leading to a disease. hosts have developed complex immune systems to cope with the danger. preceding the elimination of pathogens, selective recognition of the non-self invaders is necessary. at the forefront of the body's defenses are the innate immune cells, which are equipped with particular sensor molecules that can detect common exterior patterns of invading pathogens and their secreting toxins as well as with ... | 2016 | 26960533 |
| proton pump inhibitors and gastroenteritis. | an association between proton pump inhibitor (ppi) therapy and bacterial gastroenteritis has been suggested as well as contradicted. the aim of this study was to examine the association between the use of ppis and occurrence of bacterial gastroenteritis in the prospective rotterdam study. the rotterdam study is a population-based cohort study among 14,926 subjects aged 45 years and older with up to 24 years of follow-up. analyses were performed with a generalized estimating equations method in p ... | 2016 | 26960438 |
| discovery and dissection of metabolic oscillations in the microaerobic nitric oxide response network of escherichia coli. | the virulence of many pathogens depends upon their ability to cope with immune-generated nitric oxide (no·). in escherichia coli, the major no· detoxification systems are hmp, an no· dioxygenase (nod), and norv, an no· reductase (nor). it is well established that hmp is the dominant system under aerobic conditions, whereas norv dominates anaerobic conditions; however, the quantitative contributions of these systems under the physiologically relevant microaerobic regime remain ill defined. here, ... | 2016 | 26951670 |
| gastrointestinal disorders associated with common variable immune deficiency (cvid) and chronic granulomatous disease (cgd). | common variable immune deficiency (cvid) and chronic granulomatous disease (cgd) are two of the well-characterized primary immune deficiencies with distinct pathologic defects. while cvid is predominantly a disorder of the adaptive immune system, in cgd, innate immunity is impaired. in both syndromes, the clinical manifestations include an increased susceptibility to infections and a number of non-infectious, inflammatory conditions including systemic autoimmunity, as well as organ-specific path ... | 2016 | 26951230 |
| correction: updated campylobacter jejuni capsule pcr multiplex typing system and its application to clinical isolates from south and southeast asia. | 2016 | 26950938 | |
| cheese microbial risk assessments - a review. | cheese is generally considered a safe and nutritious food, but foodborne illnesses linked to cheese consumption have occurred in many countries. several microbial risk assessments related to listeria monocytogenes, staphylococcus aureus, and escherichia coli infections, causing cheese-related foodborne illnesses, have been conducted. although the assessments of microbial risk in soft and low moisture cheeses such as semi-hard and hard cheeses have been accomplished, it has been more focused on t ... | 2016 | 26950859 |
| transcriptome landscape of lactococcus lactis reveals many novel rnas including a small regulatory rna involved in carbon uptake and metabolism. | rna sequencing has revolutionized genome-wide transcriptome analyses, and the identification of non-coding regulatory rnas in bacteria has thus increased concurrently. here we reveal the transcriptome map of the lactic acid bacterial paradigm lactococcus lactis mg1363 by employing differential rna sequencing (drna-seq) and a combination of manual and automated transcriptome mining. this resulted in a high-resolution genome annotation of l. lactis and the identification of 60 cis-encoded antisens ... | 2016 | 26950529 |
| validation of a high-throughput immunobead array technique for multiplex detection of three foodborne pathogens in chicken products. | this study rigorously evaluated a previously developed immunobead array method to simultaneously detect three important foodborne pathogens, campylobacter jejuni, listeria monocytogenes, and salmonella spp., for its actual application in routine food testing. due to the limitation of the detection limit of the developed method, an enrichment step was included in this study by using campylobacter enrichment broth for c. jejuni and universal pre-enrichment broth for l. monocytogenes and salmonella ... | 2016 | 26950032 |
| the efficacy of a commercial competitive exclusion product on campylobacter colonization in broiler chickens in a 5-week pilot-scale study. | the efficacy of the commercial competitive exclusion product broilact against campylobacter jejuni was evaluated in broiler chickens in a 5-week pilot-scale study. newly-hatched broiler chicks were brought from a commercial hatchery. after arrival 50 seeder chicks were challenged orally with approximately 10(3) cfu of c. jejuni, wing marked, and placed back in a delivery box and moved to a separate room. the rest of the chicks (contact chicks) were placed in floor pens, 100 chicks per pen. birds ... | 2016 | 26944963 |
| sialic acid-modified antigens impose tolerance via inhibition of t-cell proliferation and de novo induction of regulatory t cells. | sialic acids are negatively charged nine-carbon carboxylated monosaccharides that often cap glycans on glycosylated proteins and lipids. because of their strategic location at the cell surface, sialic acids contribute to interactions that are critical for immune homeostasis via interactions with sialic acid-binding ig-type lectins (siglecs). in particular, these interactions may be of importance in cases where sialic acids may be overexpressed, such as on certain pathogens and tumors. we now dem ... | 2016 | 26941238 |
| the effects of 405-nm visible light on the survival of campylobacter on chicken skin and stainless steel. | campylobacter spp. are foodborne pathogens responsible for a significant portion of human cases of bacterial-mediated gastrointestinal disease. a primary method for the introduction of campylobacter into the food supply is through poultry products. reducing the number of campylobacter on poultry products may reduce the incidence of human disease. research has been conducted on the use of light to inactivate campylobacter on poultry products and processing environments. more recently, the use of ... | 2016 | 26938455 |
| c1q-targeted inhibition of the classical complement pathway prevents injury in a novel mouse model of acute motor axonal neuropathy. | guillain-barré syndrome (gbs) is an autoimmune disease that results in acute paralysis through inflammatory attack on peripheral nerves, and currently has limited, non-specific treatment options. the pathogenesis of the acute motor axonal neuropathy (aman) variant is mediated by complement-fixing anti-ganglioside antibodies that directly bind and injure the axon at sites of vulnerability such as nodes of ranvier and nerve terminals. consequently, the complement cascade is an attractive target to ... | 2016 | 26936605 |
| application of alternative nucleic acid extraction protocols to progastro sscs assay for detection of bacterial enteric pathogens. | as an alternative to automated extraction, fecal specimens were processed by investigational lysis/heating (i.e., manual) and by chromatography/centrifugation (i.e., column) methods. progastro ssc and shiga toxin-producing escherichia coli (i.e., stec) indeterminate rates for 101 specimens were 1.0% to 3.0% for automated, 11.9% for manual, and 24.8% to 37.6% for column methods. following freeze-thaw of 247 specimens, indeterminate rates were 1.6% to 2.4% for manual and 0.8 to 5.3% for column met ... | 2016 | 26935731 |
| structural model of feob, the iron transporter from pseudomonas aeruginosa, predicts a cysteine lined, gtp-gated pore. | iron is essential for the survival and virulence of pathogenic bacteria. the feob transporter allows the bacterial cell to acquire ferrous iron from its environment, making it an excellent drug target in intractable pathogens. the protein consists of an n-terminal gtp-binding domain and a c-terminal membrane domain. despite the availability of x-ray crystal structures of the n-terminal domain, many aspects of the structure and function of feob remain unclear, such as the structure of the membran ... | 2016 | 26934982 |
| horizontal dna transfer mechanisms of bacteria as weapons of intragenomic conflict. | horizontal dna transfer (hdt) is a pervasive mechanism of diversification in many microbial species, but its primary evolutionary role remains controversial. much recent research has emphasised the adaptive benefit of acquiring novel dna, but here we argue instead that intragenomic conflict provides a coherent framework for understanding the evolutionary origins of hdt. to test this hypothesis, we developed a mathematical model of a clonally descended bacterial population undergoing hdt through ... | 2016 | 26934590 |
| assessment of yeast saccharomyces cerevisiae component binding to mycobacterium avium subspecies paratuberculosis using bovine epithelial cells. | since yeast saccharomyces cerevisiae and its components are being used for the prevention and treatment of enteric diseases in different species, they may also be useful for preventing johne's disease, a chronic inflammatory bowel disease of ruminants caused by mycobacterium avium spp. paratuberculosis (map). this study aimed to identify potential yeast derivatives that may be used to help prevent map infection. the adherence of mcherry-labeled map to bovine mammary epithelial cell line (mac-t c ... | 2016 | 26932223 |
| deletion of the entire interferon-γ receptor 1 gene causing complete deficiency in three related patients. | complete interferon-γ receptor 1 (ifn-γr1) deficiency is a primary immunodeficiency causing predisposition to severe infection due to intracellular pathogens. only 36 cases have been reported worldwide. the purpose of this article is to describe a large novel deletion found in 3 related cases, which resulted in the complete removal of the ifngr1 gene. | 2016 | 26931784 |
| neisseria meningitidis polynucleotide phosphorylase affects aggregation, adhesion, and virulence. | neisseria meningitidis autoaggregation is an important step during attachment to human cells. aggregation is mediated by type iv pili and can be modulated by accessory pilus proteins, such as pilx, and posttranslational modifications of the major pilus subunit pile. the mechanisms underlying the regulation of aggregation remain poorly characterized. polynucleotide phosphorylase (pnpase) is a 3'-5' exonuclease that is involved in rna turnover and the regulation of small rnas. in this study, we bi ... | 2016 | 26930706 |
| cytotoxic and inflammatory responses induced by outer membrane vesicle-associated biologically active proteases from vibrio cholerae. | proteases in vibrio cholerae have been shown to play a role in its pathogenesis. v. cholerae secretes zn-dependent hemagglutinin protease (hap) and calcium-dependent trypsin-like serine protease (vesc) by using the type ii secretion system (tiiss). our present studies demonstrated that these proteases are also secreted in association with outer membrane vesicles (omvs) and transported to human intestinal epithelial cells in an active form. omv-associated hap induces dose-dependent apoptosis in i ... | 2016 | 26930702 |
| use of h-index and other bibliometric indicators to evaluate research productivity outcome on swine diseases. | h-index is the most commonly applied tool to evaluate scientific productivity. in this study, the use of the h-index to evaluate scientific production in swine veterinary medicine was explored. a database of 137 pig infectious agents was constructed, including its taxonomic division, zoonotic potential, status as emerging pathogen and whether it was oie-listed. the h-index and the total number of citations were calculated for those pathogens, the location of the affiliation of the first author o ... | 2016 | 26930283 |
| comparative genomic analysis of mannheimia haemolytica from bovine sources. | bovine respiratory disease is a common health problem in beef production. the primary bacterial agent involved, mannheimia haemolytica, is a target for antimicrobial therapy and at risk for associated antimicrobial resistance development. the role of m. haemolytica in pathogenesis is linked to serotype with serotypes 1 (s1) and 6 (s6) isolated from pneumonic lesions and serotype 2 (s2) found in the upper respiratory tract of healthy animals. here, we sequenced the genomes of 11 strains of m. hae ... | 2016 | 26926339 |
| fluoroquinolone and macrolide resistance in campylobacter jejuni isolated from broiler slaughterhouses in southern brazil. | campylobacter jejuni is recognized as a leading cause of acute bacterial gastroenteritis in humans. the over-use of antimicrobials in the human population and in animal husbandry has led to an increase in antimicrobial-resistant infections, particularly with fluoroquinolones and macrolides. the aim of the present study was to provide information of the current status of antimicrobial resistance patterns in campylobacter jejuni from poultry sources. fifty strains were recovered from broiler slaug ... | 2016 | 26925976 |
| ebola or not? evaluating the ill traveler from ebola-affected countries in west africa. | background. the 2014-2015 ebola epidemic in west africa had global impact beyond the primarily affected countries of guinea, liberia, and sierra leone. other countries, including the united states, encountered numerous patients who arrived from highly affected countries with fever or other signs or symptoms consistent with ebola virus disease (evd). methods. we describe our experience evaluating 25 travelers who met the us centers for disease control and prevention case definition for a person ... | 2016 | 26925428 |
| structural modeling of the flagellum ms ring protein flif reveals similarities to the type iii secretion system and sporulation complex. | the flagellum is a large proteinaceous organelle found at the surface of many bacteria, whose primary role is to allow motility through the rotation of a long extracellular filament. it is an essential virulence factor in many pathogenic species, and is also a priming component in the formation of antibiotic-resistant biofilms. the flagellum consists of the export apparatus on the cytosolic side; the basal body and rotor, spanning the bacterial membrane(s) and periplasm; and the hook-filament, t ... | 2016 | 26925337 |
| cell wall anchoring of the campylobacter antigens to lactococcus lactis. | campylobacter jejuni is the most frequent cause of human food-borne gastroenteritis and chicken meat is the main source of infection. recent studies showed that broiler chicken immunization against campylobacter should be the most efficient way to lower the number of human infections by this pathogen. induction of the mucosal immune system after oral antigen administration should provide protective immunity to chickens. in this work we tested the usefulness of lactococcus lactis, the most extens ... | 2016 | 26925040 |
| anti-gq1b antibody syndrome presenting as acute isolated bilateral ophthalmoplegia: report on two patients and review of the literature. | miller fisher syndrome (mfs) is an acute polyradiculoneuritis regarded as an uncommon clinical variant of guillain-barré syndrome (gbs). mfs is characterized by the acute onset of the clinical triad of ophthalmoplegia, cereballar ataxia and areflexia. atypical forms of mfs presenting as isolated ophthalmoplegia without ataxia have been rarely described, mostly in adults. | 2016 | 26924168 |
| the crystal structure of the tandem-pas sensing domain of campylobacter jejuni chemoreceptor tlp1 suggests indirect mechanism of ligand recognition. | chemotaxis and motility play an important role in the colonisation of avian and human hosts by campylobacter jejuni. chemotactic recognition of extracellular signals is mediated by the periplasmic sensing domain of methyl-accepting chemotactic proteins (membrane-embedded receptors). in this work, we report a high-resolution structure of the periplasmic sensing domain of transducer-like protein 1 (tlp1), an aspartate receptor of c. jejuni. crystallographic analysis revealed that it contains two p ... | 2016 | 26923153 |
| isothiocyanates as effective agents against enterohemorrhagic escherichia coli: insight to the mode of action. | production of shiga toxins by enterohemorrhagic escherichia coli (ehec) which is responsible for the pathogenicity of these strains, is strictly correlated with induction of lambdoid bacteriophages present in the host's genome, replication of phage dna and expression of stx genes. antibiotic treatment of ehec infection may lead to induction of prophage into a lytic development, thus increasing the risk of severe complications. this, together with the spread of multi-drug resistance, increases th ... | 2016 | 26922906 |
| evaluation of flagellum-related proteins flid and fspa as subunit vaccines against campylobacter jejuni colonisation in chickens. | campylobacter is the leading cause of food-borne diarrhoea in humans in the developed world and consumption of contaminated poultry meat is the main source of infection. vaccination of broilers could reduce carcass contamination and zoonotic infections. towards this aim, we evaluated recombinant anti-campylobacter subunit vaccines based on the flagellum-capping protein flid and the flagellum-secreted protein fspa as they are immunogenic in chickens and the flagellum is vital for colonisation. in ... | 2016 | 26921781 |
| packaging of campylobacter jejuni into multilamellar bodies by the ciliate tetrahymena pyriformis. | campylobacter jejuniis the leading cause of bacterial gastroenteritis worldwide. transmission to humans occurs through consumption of contaminated food or water. the conditions affecting the persistence of c. jejuniin the environment are poorly understood. some protozoa package and excrete bacteria into multilamellar bodies (mlbs). packaged bacteria are protected from deleterious conditions, which increases their survival. we hypothesized that c. jejuni could be packaged under aerobic conditions ... | 2016 | 26921427 |
| probing polytopic membrane protein-substrate interactions by luminescence resonance energy transfer. | integral membrane proteins play essential roles in all living systems; however, major technical hurdles challenge analyses of this class of proteins. biophysical approaches that provide structural information to complement and leverage experimentally determined and computationally predicted structures are urgently needed. herein we present the application of luminescence resonance energy transfer (lret) for investigating the interactions of the polytopic membrane-bound oligosaccharyl transferase ... | 2016 | 26918528 |
| successful conservative management of campylobacter cholecystitis occurring post chemotherapy and rituximab: a rare disease entity. | campylobacter jejuni is commonly associated with gastroenteritis, but extremely few reports worldwide link it acute cholecystitis. these infectious complications can assume menacing proportions in the immunocompromised and need careful management. we present a report of such a case from australia, successfully managed conservatively, without surgery. | 2015 | 26913915 |
| revisiting the roles of culture and culture-independent detection tests for campylobacter. | culture-independent detection tests (cidts) for campylobacter have become an area of intense controversy and confusion among laboratorians in the field of clinical microbiology. to date, the true analytical and clinical performance of stool antigen cidts versus truly optimized culture conditions is unknown. in this issue of the journal of clinical microbiology, fitzgerald and colleagues (c. fitzgerald et al., j clin microbiol 54:1209-1215, 2016, http://dx.doi.org/10.1128/jcm.01925-15) report com ... | 2016 | 26912756 |
| role of clinicogenomics in infectious disease diagnostics and public health microbiology. | clinicogenomics is the exploitation of genome sequence data for diagnostic, therapeutic, and public health purposes. central to this field is the high-throughput dna sequencing of genomes and metagenomes. the role of clinicogenomics in infectious disease diagnostics and public health microbiology was the topic of discussion during a recent symposium (session 161) presented at the 115th general meeting of the american society for microbiology that was held in new orleans, la. what follows is a co ... | 2016 | 26912755 |
| outer membrane vesicles as platform vaccine technology. | outer membrane vesicles (omvs) are released spontaneously during growth by many gram-negative bacteria. they present a range of surface antigens in a native conformation and have natural properties like immunogenicity, self-adjuvation and uptake by immune cells which make them attractive for application as vaccines against pathogenic bacteria. in particular with neisseria meningitidis, they have been investigated extensively and an omv-containing meningococcal vaccine has recently been approved ... | 2015 | 26912077 |
| long-term clinical course of post-infectious irritable bowel syndrome after shigellosis: a 10-year follow-up study. | a limited number of studies are available regarding the long-term natural history of post-infectious irritable bowel syndrome (pi-ibs). we aimed to investigate the long-term clinical course of pi-ibs. | 2016 | 26908484 |
| isolation and antimicrobial susceptibility patterns of campylobacter species among diarrheic children at jimma, ethiopia. | introduction. campylobacter is one of the leading bacterial causes of food-borne disease. the prevalence of campylobacter species resistant to antimicrobial agents is increasing. this study is intended to determine prevalence and antimicrobial susceptibility patterns of campylobacter species among under-five children with diarrhea. methodology. a cross-sectional study was conducted among 227 under-five children with diarrhea from july to october 2012 at jimma town. isolation and identification o ... | 2014 | 26904735 |
| rapid pcr detection of mycoplasma hominis, ureaplasma urealyticum, and ureaplasma parvum. | objective. we compared laboratory developed real-time pcr assays for detection of mycoplasma hominis and for detection and differentiation of ureaplasma urealyticum and parvum to culture using genitourinary specimens submitted for m. hominis and ureaplasma culture. methods. 283 genitourinary specimens received in the clinical bacteriology laboratory for m. hominis and ureaplasma species culture were evaluated. nucleic acids were extracted using the total nucleic acid kit on the magna pure 2.0. 5 ... | 2013 | 26904723 |
| hib vaccines: past, present, and future perspectives. | haemophilus influenzae type b (hib) causes many severe diseases, including epiglottitis, pneumonia, sepsis, and meningitis. in developed countries, the annual incidence of meningitis caused by bacteria is approximately 5-10 cases per population of 100,000. the hib conjugate vaccine is considered protective and safe. adjuvants, molecules that can enhance and/or regulate the fundamental immunogenicity of an antigen, comprise a wide range of diverse compounds. while earlier developments of adjuvant ... | 2016 | 26904695 |
| the two-component system cpxra negatively regulates the locus of enterocyte effacement of enterohemorrhagic escherichia coli involving σ(32) and lon protease. | enterohemorrhagic escherichia coli (ehec) is a significant cause of serious human gastrointestinal disease worldwide. ehec strains contain a pathogenicity island called the locus of enterocyte effacement (lee), which encodes virulence factors responsible for damaging the gut mucosa. the cpx envelope stress response of e. coli is controlled by a two-component system (tcs) consisting of a sensor histidine kinase (cpxa) and a cytoplasmic response regulator (cpxr). in this study, we investigated the ... | 2016 | 26904510 |
| molecular mechanisms and potential clinical applications of campylobacter jejuni cytolethal distending toxin. | cytolethal distending toxin (cdt), a genotoxin produced by campylobacter jejuni, is composed of three subunits: cdta, cdtb, and cdtc. cdtb is a dnase that causes dna double-strand breaks (dsb) in the nucleus resulting in cell cycle arrest at the g2/m stage and apoptosis. cdta and cdtc bind to cholesterol-rich microdomains on the cytoplasmic membrane, a process required for the delivery of cdtb to cells. although a unique motif associated with cholesterol-binding activity has been identified in o ... | 2016 | 26904508 |
| immunoproliferative small intestinal disease associated with overwhelming polymicrobial gastrointestinal infection with transformation to diffuse large b-cell lymphoma. | immunoproliferative small intestinal disease (ipsid) is an extra-nodal b-cell lymphoma most commonly described in the mediterranean, africa, and asia. it is associated with poverty and poor sanitation, and is rarely encountered in developed countries. a 26-year-old previously healthy, marshallese male was transferred to our facility with a 6-month history of watery diarrhea, weakness, and cachexia refractory to multiple short courses of oral antibiotics. stool cultures grew campylobacter jejuni ... | 2016 | 26903604 |
| maternal weaning modulates emotional behavior and regulates the gut-brain axis. | evidence shows that nutritional and environmental stress stimuli during postnatal period influence brain development and interactions between gut and brain. in this study we show that in rats, prevention of weaning from maternal milk results in depressive-like behavior, which is accompanied by changes in the gut bacteria and host metabolism. depressive-like behavior was studied using the forced-swim test on postnatal day (pnd) 25 in rats either weaned on pnd 21, or left with their mother until p ... | 2016 | 26903212 |
| a rapid and simple real-time pcr assay for detecting foodborne pathogenic bacteria in human feces. | a rapid, simple method for detecting foodborne pathogenic bacteria in human feces is greatly needed. here, we examined the efficacy of a method that employs a combination of a commercial pcr master mix, which is insensitive to pcr inhibitors, and a dna extraction method which used sodium dodecyl benzene sulfonate (sdbs), and tween 20 to counteract the inhibitory effects of sdbs on the pcr assay. this method could detect the target genes (stx1 and stx2 of enterohemorrhagic escherichia coli, inva ... | 2016 | 26902211 |
| reduced soluble rage is associated with disease severity of axonal guillain-barré syndrome. | soluble receptor for advanced glycation end products (srage) is an anti-inflammatory factor that mitigates the proinflammatory effects of high mobility group box 1 (hmgb1). the aim of this study was to investigate whether guillain-barré syndrome (gbs)-related inflammation are mediated by srage and hmgb1. we measured serum srage, hmgb1, il-6, and tnf-α levels in 86 patients with gbs and analysed associations between srage or hmgb1 and clinical variables in these subjects. in addition, we determin ... | 2016 | 26902096 |
| inflammation in irritable bowel syndrome: myth or new treatment target? | low-grade intestinal inflammation plays a key role in the pathophysiology of irritable bowel syndrome (ibs), and this role is likely to be multifactorial. the aim of this review was to summarize the evidence on the spectrum of mucosal inflammation in ibs, highlighting the relationship of this inflammation to the pathophysiology of ibs and its connection to clinical practice. we carried out a bibliographic search in medline and the cochrane library for the period of january 1966 to december 2014, ... | 2016 | 26900287 |
| bayesian techniques for comparison of the test performance of pcr and culture for the identification of campylobacter in enriched comminuted chicken samples. | using bayesian methods that do not require the definition of a gold standard, the diagnostic sensitivity and specificity of a real-time polymerase chain reaction (pcr) assay are compared to those of an enriched culture assay for detection of campylobacter in enriched comminuted chicken samples. | 2016 | 26895234 |
| distribution and genetic profiles of campylobacter in commercial broiler production from breeder to slaughter in thailand. | poultry and poultry products are commonly considered as the major vehicle of campylobacter infection in humans worldwide. to reduce the number of human cases, the epidemiology of campylobacter in poultry must be better understood. therefore, the objective of the present study was to determine the distribution and genetic relatedness of campylobacter in the thai chicken production industry. during june to october 2012, entire broiler production processes (i.e., breeder flock, hatchery, broiler fa ... | 2016 | 26886590 |
| spotyping: fast and accurate in silico mycobacterium spoligotyping from sequence reads. | spotyping is a fast and accurate program for in silico spoligotyping of mycobacterium tuberculosis isolates from next-generation sequencing reads. this novel method achieves high accuracy for reads of both uniform and varying lengths, and is about 20 to 40 times faster than spolpred. spotyping also integrates the function of producing a report summarizing associated epidemiological data from a global database of all isolates having the same spoligotype. spotyping is freely available at: https:// ... | 2016 | 26883915 |
| contributions of sinorhizobium meliloti transcriptional regulator dksa to bacterial growth and efficient symbiosis with medicago sativa. | the stringent response, mediated by the (p)ppgpp synthetase rela and the rna polymerase-binding protein dksa, is triggered by limiting nutrient conditions. for some bacteria, it is involved in regulation of virulence. we investigated the role of two dksa-like proteins from the gram-negative nitrogen-fixing symbiont sinorhizobium meliloti in free-living culture and in interaction with its host plant medicago sativa the two paralogs, encoded by the genes smc00469 and smc00049, differ in the consti ... | 2016 | 26883825 |
| the campylobacter jejuni ferric uptake regulator promotes acid survival and cross-protection against oxidative stress. | campylobacter jejuni is a prevalent cause of bacterial gastroenteritis in humans worldwide. the mechanisms by which c. jejuni survives stomach acidity remain undefined. in the present study, we demonstrated that the c. jejuni ferric uptake regulator (fur) plays an important role in c. jejuni acid survival and acid-induced cross-protection against oxidative stress. a c. jejuni δfur mutant was more sensitive to acid than the wild-type strain. profiling of the acid stimulon of the c. jejuni δfur mu ... | 2016 | 26883589 |
| [multilocus sequence typing of campylobacter jejuni isolates from guizhou province]. | 2015 | 26882572 | |
| mechanisms of resistance to aminoglycoside antibiotics: overview and perspectives. | aminoglycoside (ag) antibiotics are used to treat many gram-negative and some gram-positive infections and, importantly, multidrug-resistant tuberculosis. among various bacterial species, resistance to ags arises through a variety of intrinsic and acquired mechanisms. the bacterial cell wall serves as a natural barrier for small molecules such as ags and may be further fortified via acquired mutations. efflux pumps work to expel ags from bacterial cells, and modifications here too may cause furt ... | 2015 | 26877861 |
| mechanisms of resistance to aminoglycoside antibiotics: overview and perspectives. | aminoglycoside (ag) antibiotics are used to treat many gram-negative and some gram-positive infections and, importantly, multidrug-resistant tuberculosis. among various bacterial species, resistance to ags arises through a variety of intrinsic and acquired mechanisms. the bacterial cell wall serves as a natural barrier for small molecules such as ags and may be further fortified via acquired mutations. efflux pumps work to expel ags from bacterial cells, and modifications here too may cause furt ... | 2015 | 26877861 |
| genetic diversity of campylobacter jejuni and campylobacter coli isolates from conventional broiler flocks and the impacts of sampling strategy and laboratory method. | the genetic diversity of campylobacter jejuni and campylobacter coliisolates from commercial broiler farms was examined by multilocus sequence typing (mlst), with an assessment of the impact of the sample type and laboratory method on the genotypes of campylobacter isolated. a total of 645c. jejuniand 106c. coli isolates were obtained from 32 flocks and 17 farms, with 47 sequence types (sts) identified. the campylobacter jejuniisolates obtained by different sampling approaches and laboratory met ... | 2016 | 26873321 |
| the epigenomic landscape of prokaryotes. | dna methylation acts in concert with restriction enzymes to protect the integrity of prokaryotic genomes. studies in a limited number of organisms suggest that methylation also contributes to prokaryotic genome regulation, but the prevalence and properties of such non-restriction-associated methylation systems remain poorly understood. here, we used single molecule, real-time sequencing to map dna modifications including m6a, m4c, and m5c across the genomes of 230 diverse bacterial and archaeal ... | 2016 | 26870957 |
| multilocus sequence typing and antimicrobial resistance of campylobacter jejuni isolated from dairy calves in austria. | human campylobacteriosis is primarily associated with poultry but also cattle. in this study, 55 campylobacter jejuni strains isolated from 382 dairy calves' feces were differentiated by multilocus sequence typing and tested for antimicrobial resistance. the most prevalent sequence type (st) was st883 (20.0%), followed by st48 (14.5%), and st50 (9.1%). in contrast to st48 and st50, st883 has rarely been described in cattle previously. furthermore, risk factor analysis was performed for the prese ... | 2016 | 26870027 |
| a biotin biosynthesis gene restricted to helicobacter. | in most bacteria the last step in synthesis of the pimelate moiety of biotin is cleavage of the ester bond of pimeloyl-acyl carrier protein (acp) methyl ester. the paradigm cleavage enzyme is escherichia coli bioh which together with the bioc methyltransferase allows synthesis of the pimelate moiety by a modified fatty acid biosynthetic pathway. analyses of the extant bacterial genomes showed that bioh is absent from many bioc-containing bacteria and is replaced by other genes. helicobacter pylo ... | 2016 | 26868423 |
| isph-rps1 and isph-ubia: "rosetta stone" proteins. | the protein isph, (e)-1-hydroxy-2-methyl-but-2-enyl 4-diphosphate (hmppp) reductase, is an essential 4fe-4s cluster-containing protein in the methylerythritol phosphate pathway for isoprenoid biosynthesis. using a sequence similarity network we found that there are >400 isph proteins that are about twice as large as most of the isphs studied to date since their isph domains are fused to either the ribosomal protein s1 (rps1), or to a ubia (4-hydroxybenzoate octaprenyltransferase)-like protein. m ... | 2015 | 26865948 |
| evaluation of luminex xtag gastrointestinal pathogen panel assay for detection of multiple diarrheal pathogens in fecal samples in vietnam. | diarrheal disease is a complex syndrome that remains a leading cause of global childhood morbidity and mortality. the diagnosis of enteric pathogens in a timely and precise manner is important for making treatment decisions and informing public health policy, but accurate diagnosis is a major challenge in industrializing countries. multiplex molecular diagnostic techniques may represent a significant improvement over classical approaches. we evaluated the luminex xtag gastrointestinal pathogen p ... | 2016 | 26865681 |
| human campylobacteriosis in luxembourg, 2010-2013: a case-control study combined with multilocus sequence typing for source attribution and risk factor analysis. | campylobacteriosis has increased markedly in luxembourg during recent years. we sought to determine which campylobacter genotypes infect humans, where they may originate from, and how they may infect humans. multilocus sequence typing was performed on 1153 campylobacter jejuni and 136 c. coli human strains to be attributed to three putative animal reservoirs (poultry, ruminants, pigs) and to environmental water using the asymmetric island model. a nationwide case-control study (2010-2013) for do ... | 2016 | 26860258 |
| gold nanoparticles-coated su-8 for sensitive fluorescence-based detections of dna. | su-8 epoxy-based negative photoresist has been extensively employed as a structural material for fabrication of numerous biological microelectro-mechanical systems (bio-mems) or lab-on-a-chip (loc) devices. however, su-8 has a high autofluorescence level that limits sensitivity of microdevices that use fluorescence as the predominant detection workhorse. here, we show that deposition of a thin gold nanoparticles layer onto the su-8 surface significantly reduces the autofluorescence of the coated ... | 2012 | 26859400 |
| tuning of hemes b equilibrium redox potential is not required for cross-membrane electron transfer. | in biological energy conversion, cross-membrane electron transfer often involves an assembly of two hemesb the hemes display a large difference in redox midpoint potentials (δem_b), which in several proteins is assumed to facilitate cross-membrane electron transfer and overcome a barrier of membrane potential. here we challenge this assumption reporting on hemebligand mutants of cytochromebc1in which, for the first time in transmembrane cytochrome, one natural histidine has been replaced by lysi ... | 2016 | 26858251 |
| impact of mutations in dna gyrase genes on quinolone resistance in campylobacter jejuni. | amino acid substitutions providing quinolone resistance to campyloabcter jejuni have been found in the quinolone resistance-determining region of protein dna gyrase subunit a (gyra), with the highest frequency at position 86 followed by position 90. in this study, wild-type and mutant recombinant dna gyrase subunits were expressed in escherichia coli and purified using ni-nta agarose column chromatography. soluble 97 kda gyra and 87 kda dna gyrase subunit b were shown to reconstitute atp-depende ... | 2016 | 26857529 |
| morphological and bactericidal effects of amikacin, meropenem and imipenem on pseudomonas aeruginosa. | pseudomonas aeruginosa might be converted to coccoid bacteria under antibiotic stress. bacterial conversion would increase resistance to antibiotics due to changes in cell wall crosslink or decreased metabolic activity. morphology of p. aeruginosa under stress conditions (presence of antibiotics) can be changed to elongated bacilli, u shape and finally coccoid bacteria. results of several researches showed that coccoid bacteria are one of the most important aspects of drug resistance. it would b ... | 2015 | 26855743 |
| a modified bioautographic method for antibacterial component screening against anaerobic and microaerophilic bacteria. | direct bioautography is a useful method to identify antimicrobial compounds with potential therapeutic importance. because of technical limitations till now, it has been applied only for aerobic bacteria. in this work we present the modification of the original method by which antimicrobial screening of bacteria requiring modified atmosphere became feasible by direct bioautography. here we demonstrate its applicability by testing three anaerobic clostridium perfringens and three microaerophilic ... | 2016 | 26853123 |
| immunomodulatory and anti-inflammatory activities of chicken cathelicidin-2 derived peptides. | host defence peptides and derived peptides are promising classes of antimicrobial and immunomodulatory lead compounds. for this purpose we examined whether chicken cathelicidin-2 (cath-2)-derived peptides modulate the function and inflammatory response of avian immune cells. using a chicken macrophage cell line (hd11) we found that full-length cath-2 dose-dependently induced transcription of chemokines cxcli2/il-8, mcp-3 and ccli4/rantes, but not of pro-inflammatory cytokine il-1β. in addition, ... | 2016 | 26848845 |
| evolutionary genomics suggests that chev is an additional adaptor for accommodating specific chemoreceptors within the chemotaxis signaling complex. | escherichia coli and salmonella enterica are models for many experiments in molecular biology including chemotaxis, and most of the results obtained with one organism have been generalized to another. while most components of the chemotaxis pathway are strongly conserved between the two species, salmonella genomes contain some chemoreceptors and an additional protein, chev, that are not found in e. coli. the role of chev was examined in distantly related species bacillus subtilis and helicobacte ... | 2016 | 26844549 |
| to what extent is faims beneficial in the analysis of proteins? | high field asymmetric waveform ion mobility spectrometry (faims), also known as differential ion mobility spectrometry, is emerging as a tool for biomolecular analysis. in this article, the benefits and limitations of faims for protein analysis are discussed. the principles and mechanisms of faims separation of ions are described, and the differences between faims and conventional ion mobility spectrometry are detailed. protein analysis is considered from both the top-down (intact proteins) and ... | 2016 | 26843211 |
| antimicrobial activity of the manganese photoactivated carbon monoxide-releasing molecule [mn(co)3(tpa-κ(3)n)](+) against a pathogenic escherichia coli that causes urinary infections. | we set out to investigate the antibacterial activity of a new mn-based photoactivated carbon monoxide-releasing molecule (photocorm, [mn(co)3(tpa-κ(3)n)](+)) against an antibiotic-resistant uropathogenic strain (ec958) of escherichia coli. | 2016 | 26842766 |
| campylobacter jejuni and campylobacter coli in wild birds on danish livestock farms. | reducing the occurrence of campylobacteriosis is a food safety issue of high priority, as in recent years it has been the most commonly reported zoonosis in the eu. livestock farms are of particular interest, since cattle, swine and poultry are common reservoirs of campylobacter spp. the farm environment provides attractive foraging and breeding habitats for some bird species reported to carry thermophilic campylobacter spp. we investigated the campylobacter spp. carriage rates in 52 wild bird s ... | 2016 | 26842400 |
| campylobacter jejuni and campylobacter coli in wild birds on danish livestock farms. | reducing the occurrence of campylobacteriosis is a food safety issue of high priority, as in recent years it has been the most commonly reported zoonosis in the eu. livestock farms are of particular interest, since cattle, swine and poultry are common reservoirs of campylobacter spp. the farm environment provides attractive foraging and breeding habitats for some bird species reported to carry thermophilic campylobacter spp. we investigated the campylobacter spp. carriage rates in 52 wild bird s ... | 2016 | 26842400 |
| isolation, identification and antibiotic resistance of campylobacter strains isolated from domestic and free-living pigeons. | 1. the aim of this study was to evaluate the occurrence of campylobacter spp. in domestic and free-living pigeons and to evaluate the antibiotic resistance profiles. 2. the material consisted of cloacal swabs obtained from 108 homing pigeons and fresh faeces from 72 wild birds from lublin and its vicinity. the identification of strains isolated on differential/selective media for campylobacter spp. was carried out by maldi-tof and pcr. the susceptibility to antibiotics was evaluated by minimum i ... | 2016 | 26841300 |
| disease recurrence after early discontinuation of eculizumab in a patient with atypical hemolytic uremic syndrome with complement c3 i1157t mutation. | eculizumab, terminal complement inhibitor, has become the frontline treatment for atypical hemolytic uremic syndrome (ahus). however, the optimal treatment schedule has not yet been established. we describe here an ahus patient with a mutation of c3 i1157t who achieved remission with eculizumab and suffered a recurrence after eculizumab discontinuation, a clinical situation that has not been previously described in patients with c3 mutation. a 9-year-old male experienced an onset of ahus after v ... | 2016 | 26840081 |
| mgl receptor and immunity: when the ligand can make the difference. | c-type lectin receptors (clrs) on antigen-presenting cells (apcs) facilitate uptake of carbohydrate antigens for antigen presentation, modulating the immune response in infection, homeostasis, autoimmunity, allergy, and cancer. in this review, we focus on the role of the macrophage galactose type c-type lectin (mgl) in the immune response against self-antigens, pathogens, and tumor associated antigens (taa). mgl is a clr exclusively expressed by dendritic cells (dcs) and activated macrophages (m ... | 2015 | 26839900 |
| estimation of concentration ratio of indicator to pathogen-related gene in environmental water based on left-censored data. | a stochastic model for estimating the ratio between a fecal indicator and a pathogen based on left-censored data, which includes a substantially high number of non-detects, was constructed. river water samples were taken for 16 months at six points in a river watershed, and conventional fecal indicators (total coliforms and general escherichia coli), genetic markers (bacteroides spp.), and virulence genes (eaea of enteropathogenic e. coli and ciab of campylobacter jejuni) were quantified. the qu ... | 2016 | 26837826 |
| biomimetic design results in a potent allosteric inhibitor of dihydrodipicolinate synthase from campylobacter jejuni. | dihydrodipicolinate synthase (dhdps), an enzyme required for bacterial peptidoglycan biosynthesis, catalyzes the condensation of pyruvate and β-aspartate semialdehyde (asa) to form a cyclic product which dehydrates to form dihydrodipicolinate. dhdps has, for several years, been considered a putative target for novel antibiotics. we have designed the first potent inhibitor of this enzyme by mimicking its natural allosteric regulation by lysine, and obtained a crystal structure of the protein-inhi ... | 2016 | 26836694 |
| magnetic resonance imaging differential diagnosis of brainstem lesions in children. | differential diagnosis of brainstem lesions, either isolated or in association with cerebellar and supra-tentorial lesions, can be challenging. knowledge of the structural organization is crucial for the differential diagnosis and establishment of prognosis of pathologies with involvement of the brainstem. familiarity with the location of the lesions in the brainstem is essential, especially in the pediatric population. magnetic resonance imaging (mri) is the most sensitive and specific imaging ... | 2016 | 26834941 |
| the sos response master regulator lexa regulates the gene transfer agent of rhodobacter capsulatus and represses transcription of the signal transduction protein ccka. | the gene transfer agent of rhodobacter capsulatus (rcgta) is a genetic exchange element that combines central aspects of bacteriophage-mediated transduction and natural transformation. rcgta particles resemble a small double-stranded dna bacteriophage, package random ∼4-kb fragments of the producing cell genome, and are released from a subpopulation (<1%) of cells in a stationary-phase culture. rcgta particles deliver this dna to surrounding r. capsulatus cells, and the dna is integrated into th ... | 2016 | 26833411 |
| helicobacter pylori colonization in nepal; assessment of prevalence and potential risk factors in a hospital-based patient cohort. | helicobacter pylori, a gram-negative bacterium, can cause gastritis, peptic and duodenal ulcers. it is considered an important public health problem for both developed and developing world. this bacterium is classified as the class 1 carcinogen because it can cause cancer. | 2016 | 26833400 |
| colonization pattern of c. jejuni isolates of human and avian origin and differences in the induction of immune responses in chicken. | campylobacter jejuni (c. jejuni) is the most frequently reported bacterial food-borne pathogen. poultry is regarded to be the main reservoir for human campylobacteriosis. by some authors c. jejuni is considered as a commensal of the chicken gut, but clinical signs may be observed indicating host-pathogen interaction. little is known about c. jejuni strain dependent differences in stimulation of the immune response in chicken. therefore we investigated the colonization pattern as well as humoral ... | 2016 | 26827832 |
| onset of ulcerative colitis during a low-carbohydrate weight-loss diet and treatment with a plant-based diet: a case report. | overweight and obesity are global health concerns. various effective weight-loss diets have been developed, including the atkins diet. the atkins diet is known as an extreme low-carbohydrate diet. this diet reduces body weight and has gained widespread popularity. however, the metabolite profiles of such a diet have been shown to be detrimental to colonic health. therefore, a concern for the long-term health effects of this diet exists. we encountered a case in which ulcerative colitis developed ... | 2016 | 26824967 |
| nitazoxanide inhibits pilus biogenesis by interfering with folding of the usher protein in the outer membrane. | many bacterial pathogens assemble surface fibers termed pili or fimbriae that facilitate attachment to host cells and colonization of host tissues. the chaperone/usher (cu) pathway is a conserved secretion system that is responsible for the assembly of virulence-associated pili by many different gram-negative bacteria. pilus biogenesis by the cu pathway requires a dedicated periplasmic chaperone and an integral outer membrane (om) assembly and secretion platform termed the usher. nitazoxanide (n ... | 2016 | 26824945 |